JPS56142449A - Engyme electrode - Google Patents
Engyme electrodeInfo
- Publication number
- JPS56142449A JPS56142449A JP4708080A JP4708080A JPS56142449A JP S56142449 A JPS56142449 A JP S56142449A JP 4708080 A JP4708080 A JP 4708080A JP 4708080 A JP4708080 A JP 4708080A JP S56142449 A JPS56142449 A JP S56142449A
- Authority
- JP
- Japan
- Prior art keywords
- fixing
- membrane
- catalase
- enzyme
- hydrogen peroxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To improve response rectilinear characteristics, by fixing enzyme, which reacts an objective substrate to form H2O2, on hydrogen peroxide detecting electrode side of porous body membrane and also fixing catalase on the other side. CONSTITUTION:A hydrogen peroxide detecting electrode 4 is prepared by coating platinum on surface of a negative glass by thermal decomposition process and providing electric connection by silver paste. Polycarbonate porous membrane is used as an enzyme fixing carrier membrane 2, a mixture solution of albumin-added catalase and ascorbic acid oxidase is applied onto one side of this membrane, it is made to react in vapor of glutaraldehyde for fixing of bridge and formation of a catalase fixing layer 1, and a glucose oxidase enzyme fixing layer 3 is formed on the surface on the other side. Glucose oxidase side of thus obtained enzyme fixing membrane is tightly fixed to the hydrogen peroxide detecting electrode 4. By doing so, H2O2 is decomposed to oxygen by the catalase layer, and therefore, rectilinear characteristic in high-density region can be improved.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4708080A JPS56142449A (en) | 1980-04-09 | 1980-04-09 | Engyme electrode |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4708080A JPS56142449A (en) | 1980-04-09 | 1980-04-09 | Engyme electrode |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS56142449A true JPS56142449A (en) | 1981-11-06 |
JPS6319818B2 JPS6319818B2 (en) | 1988-04-25 |
Family
ID=12765189
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4708080A Granted JPS56142449A (en) | 1980-04-09 | 1980-04-09 | Engyme electrode |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS56142449A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61128152A (en) * | 1984-11-27 | 1986-06-16 | Hitachi Ltd | Biochemical sensor |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5481177A (en) * | 1977-12-13 | 1979-06-28 | Omron Tateisi Electronics Co | Electrode having double fixed enzyme layers |
-
1980
- 1980-04-09 JP JP4708080A patent/JPS56142449A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5481177A (en) * | 1977-12-13 | 1979-06-28 | Omron Tateisi Electronics Co | Electrode having double fixed enzyme layers |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61128152A (en) * | 1984-11-27 | 1986-06-16 | Hitachi Ltd | Biochemical sensor |
Also Published As
Publication number | Publication date |
---|---|
JPS6319818B2 (en) | 1988-04-25 |
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