JPS5574796A - Separation of acidic polysaccharides - Google Patents

Separation of acidic polysaccharides

Info

Publication number
JPS5574796A
JPS5574796A JP14650878A JP14650878A JPS5574796A JP S5574796 A JPS5574796 A JP S5574796A JP 14650878 A JP14650878 A JP 14650878A JP 14650878 A JP14650878 A JP 14650878A JP S5574796 A JPS5574796 A JP S5574796A
Authority
JP
Japan
Prior art keywords
solution
hyaluronic acid
chondroitin sulfate
treated
animal tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14650878A
Other languages
Japanese (ja)
Other versions
JPS6121241B2 (en
Inventor
Akio Hamai
Katsuyuki Horie
Toshijiro Yamaguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Priority to JP14650878A priority Critical patent/JPS5574796A/en
Publication of JPS5574796A publication Critical patent/JPS5574796A/en
Publication of JPS6121241B2 publication Critical patent/JPS6121241B2/ja
Granted legal-status Critical Current

Links

Abstract

PURPOSE: To separate hyaluronic acid and chondroitin sulfate from animal tissue, easily, in high yield, by heating the animal tissue in a solution of a quaternary ammonium salt, and treating with protease to separate hyaluronic acid, and then treating the residue with a salt solution to obtain chondroitin sulfate.
CONSTITUTION: Animal tissue such as cockscombs, naval cords, skin, etc. is heated in an aqueous solution of a quaternary ammonium slat, cut to pieces, added with water, and treated with protease. The treated solution is filtered to concentrate hyaluronic acid in the filtrate (solution A). The residue is treated with a solution of a salt such as NaCl or of an alkali such as NaOH, to collect chondroitin sulfate in the filtrate (solution B). The hyaluronic acid and the chondroitin sulfate are precipitated by adding an alcohol to the solutions A and B.
COPYRIGHT: (C)1980,JPO&Japio
JP14650878A 1978-11-29 1978-11-29 Separation of acidic polysaccharides Granted JPS5574796A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14650878A JPS5574796A (en) 1978-11-29 1978-11-29 Separation of acidic polysaccharides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14650878A JPS5574796A (en) 1978-11-29 1978-11-29 Separation of acidic polysaccharides

Publications (2)

Publication Number Publication Date
JPS5574796A true JPS5574796A (en) 1980-06-05
JPS6121241B2 JPS6121241B2 (en) 1986-05-26

Family

ID=15409207

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14650878A Granted JPS5574796A (en) 1978-11-29 1978-11-29 Separation of acidic polysaccharides

Country Status (1)

Country Link
JP (1) JPS5574796A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000035959A1 (en) * 1998-12-17 2000-06-22 Fujii Suisan Co., Ltd. Method for processing fish heads and apparatus for separating processed fish heads
JP2010124811A (en) * 2008-12-01 2010-06-10 Daimatsu:Kk Volume reduction extraction method of glycosaminoglycan and generation method of proteoglycan-containing precipitation

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6371829U (en) * 1986-10-30 1988-05-13

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000035959A1 (en) * 1998-12-17 2000-06-22 Fujii Suisan Co., Ltd. Method for processing fish heads and apparatus for separating processed fish heads
US6347986B1 (en) 1998-12-17 2002-02-19 Fujii Suisan Co., Ltd. Method for processing fish heads and apparatus for separating processed fish heads
JP2010124811A (en) * 2008-12-01 2010-06-10 Daimatsu:Kk Volume reduction extraction method of glycosaminoglycan and generation method of proteoglycan-containing precipitation

Also Published As

Publication number Publication date
JPS6121241B2 (en) 1986-05-26

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