JPS5530655A - Latex sensitive to human igg antibody for quantitizing human igg and its particle composite - Google Patents
Latex sensitive to human igg antibody for quantitizing human igg and its particle compositeInfo
- Publication number
- JPS5530655A JPS5530655A JP10389378A JP10389378A JPS5530655A JP S5530655 A JPS5530655 A JP S5530655A JP 10389378 A JP10389378 A JP 10389378A JP 10389378 A JP10389378 A JP 10389378A JP S5530655 A JPS5530655 A JP S5530655A
- Authority
- JP
- Japan
- Prior art keywords
- human igg
- latex
- igg
- buffer liquid
- quantitizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To make it possible to simply and highly sensitively quantitize the human IgG without being required to condense urine or the like by sensitizing special latex particles to human immunity globulinG(IgG) and by making the same contains special stabilizing agent.
CONSTITUTION: Human IgG is injected into rabbit or the like, and blood is sampled to obtain antiserum, from which a purified human IgG. The purified human IgG diluted with the mixture of a phosphoric salt buffer liquid and the physiological solution of sodium chloride is reacted with in a buffer liquid of pH 6.5 to 8 or polystyrene latex, which has a narrow diameter distribution of 0.01 to 10 (or 0.1 to 1.0)μ ranging from 0.2 to 0.005μ and having specific weight of 0.9 to 1.4 while having a latex particle concentration of 0.05 to 5%. After this reaction, the centrifugal separation and rinsing are performed. Finally, the latex is suspended in a dilution which is prepared by adding arbumin of cow serum and NaN3 to the aqueous solution of for buffering the phophoric acid or glycine with a view to preventing coaggulation.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10389378A JPS5530655A (en) | 1978-08-28 | 1978-08-28 | Latex sensitive to human igg antibody for quantitizing human igg and its particle composite |
CH776779A CH645726A5 (en) | 1978-08-28 | 1979-08-27 | PREPARATION FOR DETERMINING HUMAN IMMUNOGLOBULIN G, METHOD FOR THE PRODUCTION THEREOF AND ITS USE. |
GB7929796A GB2030293B (en) | 1978-08-28 | 1979-08-28 | Composition for determination of human immunoglobulin g process for its preparation and its use |
FR7921529A FR2435040A1 (en) | 1978-08-28 | 1979-08-28 | COMPOSITION FOR DETERMINING HUMAN IMMUNOGLOBULIN G, METHOD FOR ITS PREPARATION AND ITS USE |
DE19792934757 DE2934757C2 (en) | 1978-08-28 | 1979-08-28 | Composition and method for the determination of human immunoglobulin-G |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10389378A JPS5530655A (en) | 1978-08-28 | 1978-08-28 | Latex sensitive to human igg antibody for quantitizing human igg and its particle composite |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5530655A true JPS5530655A (en) | 1980-03-04 |
Family
ID=14366092
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10389378A Pending JPS5530655A (en) | 1978-08-28 | 1978-08-28 | Latex sensitive to human igg antibody for quantitizing human igg and its particle composite |
Country Status (5)
Country | Link |
---|---|
JP (1) | JPS5530655A (en) |
CH (1) | CH645726A5 (en) |
DE (1) | DE2934757C2 (en) |
FR (1) | FR2435040A1 (en) |
GB (1) | GB2030293B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56140255A (en) * | 1980-04-03 | 1981-11-02 | Terumo Corp | Detecting reagent of antibody for antiacetylcholine receptor, and its using method |
JPS59193356A (en) * | 1983-03-17 | 1984-11-01 | バイオウィッテッカー・インコーポレーテッド | Fluorescent luminous intensity analysis for total ige concentration and reagent therefor |
CN105929171A (en) * | 2016-04-13 | 2016-09-07 | 柏荣诊断产品(上海)有限公司 | High performance human urine immune globulin G detection kit |
JP2021170021A (en) * | 2016-10-03 | 2021-10-28 | ヒュービットジェノミクス株式会社 | Test method enabling diagnosis specific to early pathology of diabetic nephropathy |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4331649A (en) | 1978-10-10 | 1982-05-25 | Burroughs Wellcome Co. | Immune complex assay |
FI792576A (en) * | 1979-08-20 | 1981-02-21 | Orion Yhtymae Oy | METHOD OF ENZYME IMMUNOLOGICAL ASSESSMENT |
CA1206876A (en) * | 1982-09-13 | 1986-07-02 | Ernest C. Adams | Method for measuring igg in a neonatal foal or calf and in colostrum |
JPS59100863A (en) * | 1982-12-01 | 1984-06-11 | Fuji Seiyaku Kogyo Kk | Test slide for diagnosis and its production |
DE3303083A1 (en) * | 1983-01-31 | 1984-08-02 | Behringwerke Ag, 3550 Marburg | IMMUNOLOGICAL LATEX AGGLUTINATION METHOD AND AGENT |
EP0194156B1 (en) * | 1985-03-08 | 1990-02-21 | Sanko Junyaku Co., Ltd. | Method of measuring the amount of immune antibody in serum |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3088875A (en) * | 1959-10-27 | 1963-05-07 | Hyland Lab | Immunological diagnostics utilizing polystyrene latex particles of 0.15 to 0.25 micron |
NL125235C (en) * | 1965-04-15 | |||
GB1384399A (en) * | 1971-02-01 | 1975-02-19 | Hoffmann La Roche | Automated method of obtaining serological data |
-
1978
- 1978-08-28 JP JP10389378A patent/JPS5530655A/en active Pending
-
1979
- 1979-08-27 CH CH776779A patent/CH645726A5/en not_active IP Right Cessation
- 1979-08-28 GB GB7929796A patent/GB2030293B/en not_active Expired
- 1979-08-28 DE DE19792934757 patent/DE2934757C2/en not_active Expired
- 1979-08-28 FR FR7921529A patent/FR2435040A1/en active Granted
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56140255A (en) * | 1980-04-03 | 1981-11-02 | Terumo Corp | Detecting reagent of antibody for antiacetylcholine receptor, and its using method |
JPS59193356A (en) * | 1983-03-17 | 1984-11-01 | バイオウィッテッカー・インコーポレーテッド | Fluorescent luminous intensity analysis for total ige concentration and reagent therefor |
CN105929171A (en) * | 2016-04-13 | 2016-09-07 | 柏荣诊断产品(上海)有限公司 | High performance human urine immune globulin G detection kit |
JP2021170021A (en) * | 2016-10-03 | 2021-10-28 | ヒュービットジェノミクス株式会社 | Test method enabling diagnosis specific to early pathology of diabetic nephropathy |
Also Published As
Publication number | Publication date |
---|---|
FR2435040B1 (en) | 1984-09-21 |
CH645726A5 (en) | 1984-10-15 |
GB2030293A (en) | 1980-04-02 |
GB2030293B (en) | 1983-03-23 |
DE2934757A1 (en) | 1980-03-13 |
FR2435040A1 (en) | 1980-03-28 |
DE2934757C2 (en) | 1984-12-20 |
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