JPS5529990A - Activity measurement of monoamine oxidase (mao) - Google Patents
Activity measurement of monoamine oxidase (mao)Info
- Publication number
- JPS5529990A JPS5529990A JP3104979A JP3104979A JPS5529990A JP S5529990 A JPS5529990 A JP S5529990A JP 3104979 A JP3104979 A JP 3104979A JP 3104979 A JP3104979 A JP 3104979A JP S5529990 A JPS5529990 A JP S5529990A
- Authority
- JP
- Japan
- Prior art keywords
- mao
- monoamine oxidase
- absorbance
- nitro
- salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Pyridine Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Quinoline Compounds (AREA)
Abstract
PURPOSE: To make it possible to measure the MAO activity accurately and readily, by determining the oxidation product, formed through the enzyme reaction of an azo derivative of a specific formula or its salt as a substrate, optically.
CONSTITUTION: A solution of an azo derivative, e.g. 2-(3'-nitro-4'-aminomethylphenylazo)-m-cresol hydrochloride, of formula I: (X is aromatic group except unsubstituted phenyl group; R is group forming a chromophore with the adjacent -N=N-, e.g. unsubstituted phenyl group) or its salt as a substrate in tris (hydroxymethyl)aminomethane-HCl buffer is subjected to the enzyme reaction with blood serums, warmed and extracted with cyclohexame. The absorbance of the extract is measured by spectroscopy at a peak of 435nm due to the formed oxidation porduct, 2-(3'-nitro-4'-benzaldehydoazo)-m-cresol, and the ratio of the absorbance to that of the standard solution provides the activity of enzyme unit of monoamine oxidase (MAO) in the serums.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3104979A JPS5529990A (en) | 1979-03-19 | 1979-03-19 | Activity measurement of monoamine oxidase (mao) |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3104979A JPS5529990A (en) | 1979-03-19 | 1979-03-19 | Activity measurement of monoamine oxidase (mao) |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6411273A Division JPS5422120B2 (en) | 1973-06-07 | 1973-06-07 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5529990A true JPS5529990A (en) | 1980-03-03 |
JPS5654159B2 JPS5654159B2 (en) | 1981-12-23 |
Family
ID=12320618
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3104979A Granted JPS5529990A (en) | 1979-03-19 | 1979-03-19 | Activity measurement of monoamine oxidase (mao) |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5529990A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58130739U (en) * | 1982-02-26 | 1983-09-03 | シャープ株式会社 | Ink supply device in ink jetting device |
JPS59190968A (en) * | 1983-04-05 | 1984-10-29 | バイエル・アクチエンゲゼルシヤフト | Manufacture of symmetric 1,4-dihydropyridine dicarboxylic acid ester |
-
1979
- 1979-03-19 JP JP3104979A patent/JPS5529990A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58130739U (en) * | 1982-02-26 | 1983-09-03 | シャープ株式会社 | Ink supply device in ink jetting device |
JPS59190968A (en) * | 1983-04-05 | 1984-10-29 | バイエル・アクチエンゲゼルシヤフト | Manufacture of symmetric 1,4-dihydropyridine dicarboxylic acid ester |
Also Published As
Publication number | Publication date |
---|---|
JPS5654159B2 (en) | 1981-12-23 |
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