JPS5635998A - Measurement of amylase activity - Google Patents
Measurement of amylase activityInfo
- Publication number
- JPS5635998A JPS5635998A JP11313879A JP11313879A JPS5635998A JP S5635998 A JPS5635998 A JP S5635998A JP 11313879 A JP11313879 A JP 11313879A JP 11313879 A JP11313879 A JP 11313879A JP S5635998 A JPS5635998 A JP S5635998A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- amylase activity
- measurement
- maltooligosaccharide
- glucosidases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
PURPOSE: To measure correctly amylase activity in a sample without the influence of the impurities of the sample, by using a maltooligosaccharide wherein a halogenated phenyl group is bonded to the reductive terminals and α- and β-glucosidases as a reagent.
CONSTITUTION: A sample is treated with a compound wherein a halogenated phenol, e.g., p-chlorophenol, o-chlorophenol, etc. is bonded to the reductive terminals of maltooligosaccharide, e.g., maltotetraose, maltopentaose, etc. to which about 2W10 glucoses are linked. The sample is then reacted with α-glycosidase and β- glucosidase to liberate a halogenated phenol, which is determined so that amylase activity in the sample is measured. The sample may be treated with the glucosidases and amylase simultaneously.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11313879A JPS5913199B2 (en) | 1979-09-03 | 1979-09-03 | Amylase activity measurement method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11313879A JPS5913199B2 (en) | 1979-09-03 | 1979-09-03 | Amylase activity measurement method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5635998A true JPS5635998A (en) | 1981-04-08 |
JPS5913199B2 JPS5913199B2 (en) | 1984-03-28 |
Family
ID=14604515
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11313879A Expired JPS5913199B2 (en) | 1979-09-03 | 1979-09-03 | Amylase activity measurement method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5913199B2 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54103395A (en) * | 1978-01-31 | 1979-08-14 | Toyo Boseki | Amylase activity measuring method |
JPS5974999A (en) * | 1982-10-20 | 1984-04-27 | Toyobo Co Ltd | Method for measuring amylase activity |
EP0133329A2 (en) * | 1983-06-28 | 1985-02-20 | MERCK PATENT GmbH | Method and reagent for the determination of alpha-amylase |
JPS6078994A (en) * | 1983-10-06 | 1985-05-04 | Seishin Seiyaku Kk | Beta-(2-chloro-4-nitrophenyl)-maltopentaoside and its preparation |
EP0676475A1 (en) * | 1991-11-19 | 1995-10-11 | Nippon Shinyaku Company, Limited | Process for producing saccharide |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63127489U (en) * | 1987-02-13 | 1988-08-19 |
-
1979
- 1979-09-03 JP JP11313879A patent/JPS5913199B2/en not_active Expired
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54103395A (en) * | 1978-01-31 | 1979-08-14 | Toyo Boseki | Amylase activity measuring method |
JPS60997B2 (en) * | 1978-01-31 | 1985-01-11 | 東洋紡績株式会社 | Amylase activity measurement method |
JPS5974999A (en) * | 1982-10-20 | 1984-04-27 | Toyobo Co Ltd | Method for measuring amylase activity |
JPH0367680B2 (en) * | 1982-10-20 | 1991-10-23 | Toyo Boseki | |
EP0133329A2 (en) * | 1983-06-28 | 1985-02-20 | MERCK PATENT GmbH | Method and reagent for the determination of alpha-amylase |
JPS6078994A (en) * | 1983-10-06 | 1985-05-04 | Seishin Seiyaku Kk | Beta-(2-chloro-4-nitrophenyl)-maltopentaoside and its preparation |
JPH026760B2 (en) * | 1983-10-06 | 1990-02-13 | Seishin Seiyaku Kk | |
EP0676475A1 (en) * | 1991-11-19 | 1995-10-11 | Nippon Shinyaku Company, Limited | Process for producing saccharide |
EP0676475A4 (en) * | 1991-11-19 | 1996-02-07 | Nippon Shinyaku Co Ltd | Process for producing saccharide. |
Also Published As
Publication number | Publication date |
---|---|
JPS5913199B2 (en) | 1984-03-28 |
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