JPS5635998A - Measurement of amylase activity - Google Patents

Measurement of amylase activity

Info

Publication number
JPS5635998A
JPS5635998A JP11313879A JP11313879A JPS5635998A JP S5635998 A JPS5635998 A JP S5635998A JP 11313879 A JP11313879 A JP 11313879A JP 11313879 A JP11313879 A JP 11313879A JP S5635998 A JPS5635998 A JP S5635998A
Authority
JP
Japan
Prior art keywords
sample
amylase activity
measurement
maltooligosaccharide
glucosidases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP11313879A
Other languages
Japanese (ja)
Other versions
JPS5913199B2 (en
Inventor
Yoshitaka Nakagiri
Takaaki Kanda
Takayuki Asai
Kanemasa Inamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP11313879A priority Critical patent/JPS5913199B2/en
Publication of JPS5635998A publication Critical patent/JPS5635998A/en
Publication of JPS5913199B2 publication Critical patent/JPS5913199B2/en
Expired legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

PURPOSE: To measure correctly amylase activity in a sample without the influence of the impurities of the sample, by using a maltooligosaccharide wherein a halogenated phenyl group is bonded to the reductive terminals and α- and β-glucosidases as a reagent.
CONSTITUTION: A sample is treated with a compound wherein a halogenated phenol, e.g., p-chlorophenol, o-chlorophenol, etc. is bonded to the reductive terminals of maltooligosaccharide, e.g., maltotetraose, maltopentaose, etc. to which about 2W10 glucoses are linked. The sample is then reacted with α-glycosidase and β- glucosidase to liberate a halogenated phenol, which is determined so that amylase activity in the sample is measured. The sample may be treated with the glucosidases and amylase simultaneously.
COPYRIGHT: (C)1981,JPO&Japio
JP11313879A 1979-09-03 1979-09-03 Amylase activity measurement method Expired JPS5913199B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11313879A JPS5913199B2 (en) 1979-09-03 1979-09-03 Amylase activity measurement method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11313879A JPS5913199B2 (en) 1979-09-03 1979-09-03 Amylase activity measurement method

Publications (2)

Publication Number Publication Date
JPS5635998A true JPS5635998A (en) 1981-04-08
JPS5913199B2 JPS5913199B2 (en) 1984-03-28

Family

ID=14604515

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11313879A Expired JPS5913199B2 (en) 1979-09-03 1979-09-03 Amylase activity measurement method

Country Status (1)

Country Link
JP (1) JPS5913199B2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54103395A (en) * 1978-01-31 1979-08-14 Toyo Boseki Amylase activity measuring method
JPS5974999A (en) * 1982-10-20 1984-04-27 Toyobo Co Ltd Method for measuring amylase activity
EP0133329A2 (en) * 1983-06-28 1985-02-20 MERCK PATENT GmbH Method and reagent for the determination of alpha-amylase
JPS6078994A (en) * 1983-10-06 1985-05-04 Seishin Seiyaku Kk Beta-(2-chloro-4-nitrophenyl)-maltopentaoside and its preparation
EP0676475A1 (en) * 1991-11-19 1995-10-11 Nippon Shinyaku Company, Limited Process for producing saccharide

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63127489U (en) * 1987-02-13 1988-08-19

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54103395A (en) * 1978-01-31 1979-08-14 Toyo Boseki Amylase activity measuring method
JPS60997B2 (en) * 1978-01-31 1985-01-11 東洋紡績株式会社 Amylase activity measurement method
JPS5974999A (en) * 1982-10-20 1984-04-27 Toyobo Co Ltd Method for measuring amylase activity
JPH0367680B2 (en) * 1982-10-20 1991-10-23 Toyo Boseki
EP0133329A2 (en) * 1983-06-28 1985-02-20 MERCK PATENT GmbH Method and reagent for the determination of alpha-amylase
JPS6078994A (en) * 1983-10-06 1985-05-04 Seishin Seiyaku Kk Beta-(2-chloro-4-nitrophenyl)-maltopentaoside and its preparation
JPH026760B2 (en) * 1983-10-06 1990-02-13 Seishin Seiyaku Kk
EP0676475A1 (en) * 1991-11-19 1995-10-11 Nippon Shinyaku Company, Limited Process for producing saccharide
EP0676475A4 (en) * 1991-11-19 1996-02-07 Nippon Shinyaku Co Ltd Process for producing saccharide.

Also Published As

Publication number Publication date
JPS5913199B2 (en) 1984-03-28

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