JPS55152458A - Method of eliminating immunoreaction interference in immunological measurement method - Google Patents
Method of eliminating immunoreaction interference in immunological measurement methodInfo
- Publication number
- JPS55152458A JPS55152458A JP5974679A JP5974679A JPS55152458A JP S55152458 A JPS55152458 A JP S55152458A JP 5974679 A JP5974679 A JP 5974679A JP 5974679 A JP5974679 A JP 5974679A JP S55152458 A JPS55152458 A JP S55152458A
- Authority
- JP
- Japan
- Prior art keywords
- substance
- antibody
- bonded
- fragment
- antibody fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PURPOSE: To remove the influence of an interfering substance and measure a minute quantity of a substance in a living body fluid with high sensitivity, by using an antibody fragment which is obtained by decomposing an antibody immunoglobulin with a protease, and by adding a hydrophobic protein and salts to the antibody fragment.
CONSTITUTION: An antibody fragment 7 obtained by decomposing an antibody immunoglobulin with a protease is fixed in a solid phase 3 of silicone rubber or the like. On the other hand, a bonded substance comprising an enzyme 4 and an antibody fragment 7' bonded with each other is prepared. A living body fluid is brought into contact with the fragment 7 to bond a measured substance to the fragment 7. The bonded substance comprising the enzyme 4 and the fragment 7' is conjoined to the measured substance 1. The free bonded substance is removed by washing. The activity of the enzyme bonded to the solid phase is measured to determine the quantity of the substance 1. Even if there is a self-antibody such as a rheumatic factor 5, it is not bonded to any antibody fragment. Therefore, the self-antibody does not perform interferential action. Since a hydrophobic protein and salts are added to the antibody fragment, various factors originating from blood serum are also prevented from interfering with immunoreaction.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5974679A JPS55152458A (en) | 1979-05-17 | 1979-05-17 | Method of eliminating immunoreaction interference in immunological measurement method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5974679A JPS55152458A (en) | 1979-05-17 | 1979-05-17 | Method of eliminating immunoreaction interference in immunological measurement method |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS55152458A true JPS55152458A (en) | 1980-11-27 |
Family
ID=13122101
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP5974679A Pending JPS55152458A (en) | 1979-05-17 | 1979-05-17 | Method of eliminating immunoreaction interference in immunological measurement method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55152458A (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5899758A (en) * | 1981-12-09 | 1983-06-14 | Amano Pharmaceut Co Ltd | Determination of secretary component |
JPS59224563A (en) * | 1983-06-03 | 1984-12-17 | Toyobo Co Ltd | Reagent composition and method for measuring enzyme immunity of human chorionic gonandotropin |
JPS6069555A (en) * | 1983-09-27 | 1985-04-20 | Teijin Ltd | Immunomeasurement method using sulfonation antibody |
JPS6091263A (en) * | 1983-09-01 | 1985-05-22 | マツクス−プランク−ゲゼルシヤフト・ツ−ル・フエルデルング・デル・ヴイツセンシヤフテン・エ−・フアウ | Immunological determination method of protein in body fluid |
WO1990008320A1 (en) * | 1989-01-19 | 1990-07-26 | Teijin Limited | Immunoassay method, reagent and kit |
-
1979
- 1979-05-17 JP JP5974679A patent/JPS55152458A/en active Pending
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5899758A (en) * | 1981-12-09 | 1983-06-14 | Amano Pharmaceut Co Ltd | Determination of secretary component |
JPS59224563A (en) * | 1983-06-03 | 1984-12-17 | Toyobo Co Ltd | Reagent composition and method for measuring enzyme immunity of human chorionic gonandotropin |
JPS6091263A (en) * | 1983-09-01 | 1985-05-22 | マツクス−プランク−ゲゼルシヤフト・ツ−ル・フエルデルング・デル・ヴイツセンシヤフテン・エ−・フアウ | Immunological determination method of protein in body fluid |
JPH059745B2 (en) * | 1983-09-01 | 1993-02-05 | Matsukusu Puranku G Tsua Fueruderunku Deru Uitsusenshafuten Ee Fuau | |
JPS6069555A (en) * | 1983-09-27 | 1985-04-20 | Teijin Ltd | Immunomeasurement method using sulfonation antibody |
WO1990008320A1 (en) * | 1989-01-19 | 1990-07-26 | Teijin Limited | Immunoassay method, reagent and kit |
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