JPS57100349A - Measuring method for enzyme immunity of antibody immunoglobulin using regeneratable immune adsorption body - Google Patents

Measuring method for enzyme immunity of antibody immunoglobulin using regeneratable immune adsorption body

Info

Publication number
JPS57100349A
JPS57100349A JP17649080A JP17649080A JPS57100349A JP S57100349 A JPS57100349 A JP S57100349A JP 17649080 A JP17649080 A JP 17649080A JP 17649080 A JP17649080 A JP 17649080A JP S57100349 A JPS57100349 A JP S57100349A
Authority
JP
Japan
Prior art keywords
antibody
antibody immunoglobulin
immunoglobulin
combined
antiimmunoglobulin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP17649080A
Other languages
Japanese (ja)
Inventor
Kanefusa Kato
Akira Kosaka
Ryohei Yamamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AMANO SEIYAKU KK
Amano Enzyme Inc
Original Assignee
AMANO SEIYAKU KK
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AMANO SEIYAKU KK, Amano Pharmaceutical Co Ltd filed Critical AMANO SEIYAKU KK
Priority to JP17649080A priority Critical patent/JPS57100349A/en
Publication of JPS57100349A publication Critical patent/JPS57100349A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE:To measure an antibody immunoglobulin accurately and reuseably, by combining an antiimmunoglobulin antibody with already used inactive carrier again to restrain or to remove the interfering substances containing in a living body fluids and using it repeatedly. CONSTITUTION:An antibody immunoglobulin is combined with an antigen corresponding to the antibody immunoglobulin tagged by enzyme under the existense of hydrophobic protein and salts and produced immune chemical complex is adsorbed to the inactive antigen combined with an antiimmunoglobulin antibody by S-S bond. Next, the S-S bond is cut by reducing agent such as dithioslateol and combined body of the immue chemical complex withthe antiimmunoglobulin antibody is eluted. The accurate measurement is performed not affected by the interfering substance of the antibody immunoglobulin in the living body fluids and also, the application of automatic measuring system is achieved because a continuous use of the insoluble carrier is possible.
JP17649080A 1980-12-16 1980-12-16 Measuring method for enzyme immunity of antibody immunoglobulin using regeneratable immune adsorption body Pending JPS57100349A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17649080A JPS57100349A (en) 1980-12-16 1980-12-16 Measuring method for enzyme immunity of antibody immunoglobulin using regeneratable immune adsorption body

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17649080A JPS57100349A (en) 1980-12-16 1980-12-16 Measuring method for enzyme immunity of antibody immunoglobulin using regeneratable immune adsorption body

Publications (1)

Publication Number Publication Date
JPS57100349A true JPS57100349A (en) 1982-06-22

Family

ID=16014573

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17649080A Pending JPS57100349A (en) 1980-12-16 1980-12-16 Measuring method for enzyme immunity of antibody immunoglobulin using regeneratable immune adsorption body

Country Status (1)

Country Link
JP (1) JPS57100349A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6271862A (en) * 1986-08-30 1987-04-02 アンステイテユ、ナシヨナル、ド、ラ、サント、エ、ド、ラ、ルシエルシユ、メデイカル（アンセルム） Protein carrier modified so as to reversibly fixing antigen or immunological active substance, and application thereof to determination and purification of antigen or similar article
US4980278A (en) * 1985-05-28 1990-12-25 Olympus Optical Co., Ltd. Method of effecting immunological analysis and apparatus for carrying out the same

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4980278A (en) * 1985-05-28 1990-12-25 Olympus Optical Co., Ltd. Method of effecting immunological analysis and apparatus for carrying out the same
JPS6271862A (en) * 1986-08-30 1987-04-02 アンステイテユ、ナシヨナル、ド、ラ、サント、エ、ド、ラ、ルシエルシユ、メデイカル（アンセルム） Protein carrier modified so as to reversibly fixing antigen or immunological active substance, and application thereof to determination and purification of antigen or similar article

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