JPH1192321A - Activity inhibitor of nematode - Google Patents
Activity inhibitor of nematodeInfo
- Publication number
- JPH1192321A JPH1192321A JP26020797A JP26020797A JPH1192321A JP H1192321 A JPH1192321 A JP H1192321A JP 26020797 A JP26020797 A JP 26020797A JP 26020797 A JP26020797 A JP 26020797A JP H1192321 A JPH1192321 A JP H1192321A
- Authority
- JP
- Japan
- Prior art keywords
- coffee bean
- bean pulp
- nematodes
- nematode
- coffee grounds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、多くの野菜、花
卉、芝草、果樹さらには樹木等で報告されている、土壌
病害あるいは樹木の枯死の原因となる植物寄生性線虫に
対して有効な線虫の活性抑制剤に関する。The present invention is effective against plant parasitic nematodes, which are reported on many vegetables, flowers, turfgrass, fruit trees, and trees, and cause soil disease or tree death. The present invention relates to a nematode activity inhibitor.
【０００２】[0002]
【従来の技術】各種線虫によって惹起される土壌病害
は、多くの野菜、花卉、果樹あるいは芝草等で報告され
ており、これら植物の健全な成育を阻害する重要な病害
として認識されている。なぜなら、野菜や花卉は圃場や
ハウスで周年栽培され、連作されるため、一度病害が発
生すると大被害につながってしまうからである。果樹に
おいても同様である。芝においては、特にゴルフ場など
で、グリーンやフェアーウエイでの病害発生は、ゴルフ
場の存在価値を失うものであり、キーパーはその維持管
理に多大の努力を払っているのが現状である。また、森
林の樹木において線虫害が発生すると、病害が発生した
樹木を切り倒して焼却処分する等の対応を早急にとらな
いと森林全体に病害が波及し壊滅的な被害となる。2. Description of the Related Art Soil diseases caused by various nematodes have been reported in many vegetables, flowers, fruit trees, turfgrass and the like, and are recognized as important diseases that inhibit the healthy growth of these plants. This is because vegetables and flowers are cultivated year-round in fields and houses, and are continuously cropped, so that once a disease occurs, it leads to severe damage. The same applies to fruit trees. In the case of turf, especially in golf courses and the like, the occurrence of diseases on greens and fairways loses the existence value of golf courses, and the keeper has made great efforts to maintain and manage the grasses. In addition, if nematode damage occurs in a tree in the forest, the disease spreads to the entire forest and causes catastrophic damage unless measures such as cutting down the infested tree and incinerating it are taken immediately.
【０００３】これらの土壌病害の代表的な例は、ジャガ
イモの地下部に寄生し、根の組織を壊死させ、しいては
萎凋症状を発現させる、ジャガイモシスト線虫による
害、芝、イネ科、マメ科、ユリ科、ナス科、アカザ科、
アブラナ科、キク科、ウリ科等の作物、リンゴ、モモ、
ナシ、ブドウ、カキ、パイナップル等の果樹及び芝等の
植物の根に侵入し、ゴール（根コブ）を形成し、それに
よって根系全体が奇形となったり、岐根が生じたり、表
皮がザラついたりして、品質や貯蔵性を著しく低下させ
るサツマイモネコブ線虫による害、ニンジン、トマト、
キュウリ、ダイズ等の作物に寄生し、根の生長点付近に
侵入して品質低下をきたすキタネコブ線虫による害、ニ
ンジン、ゴボウ、ダイコン等の根菜類に多く寄生し、品
質低下や、収量低下を引き起こすミナミネグサレ線虫に
よる害、定植後２〜３年程度の若いお茶の苗に寄生し
て、成育を阻害するチャネグサレ線虫の害などが知られ
ている。[0003] Representative examples of these soil diseases are potato cyst nematodes, turf, gramineous plants, which infest the underground portion of potatoes and cause necrosis of root tissues and thus exhibit wilting symptoms. Legumes, lilies, solanaceae, red crabs,
Crops such as cruciferous, asteraceae, cucurbitaceae, apples, peaches,
Infiltrate the roots of fruits such as pears, grapes, oysters, pineapples and plants such as turf to form a goal (root knot), thereby deforming the entire root system, forming roots, and roughening the epidermis. Or the damage caused by sweet potato nematodes, carrots, tomatoes,
It is infested by crops such as cucumber and soybean, and is infested by root rots such as carrots, burdock, and radish, which invades the root growth point and causes quality deterioration. It is known that the harm caused by S. elegans causes harm and the harm of S. elegans which infests young tea seedlings about two to three years after planting and inhibits the growth.
【０００４】これらの病害に対する防除手段としては、
従来から土壌燻蒸剤による土壌消毒が不可欠とされてき
た。しかし、これら毒性の高い燻蒸剤を使用すること
は、環境汚染を引き起こしたり、作業者の健康を害した
り、さらには土壌中の有用微生物を死滅させ、結果的に
は病害菌の占有化が生じるなどの多くの問題点があり、
中には、オゾン層破壊の原因物質と指摘され、使用期限
が限定されている物質も存在する。[0004] As a control means against these diseases,
Conventionally, soil disinfection with a soil fumigant has been indispensable. However, the use of these highly toxic fumigants causes environmental pollution, harms the health of workers, and also kills useful microorganisms in the soil, resulting in the occupation of disease germs. There are many problems such as
Some of them have been pointed out as substances causing ozone layer depletion and have a limited expiration date.
【０００５】樹木に於ける被害の代表的な例は、マツノ
ザイ線虫による松ノ木の枯死であり、一度被害が発生す
ると、松枯れ病が発生した松ノ木を切り倒して焼却処分
すること以外には、有効な対策が見出せない状態にあ
る。薬剤散布による松枯れ病の防除方法は、無害な昆虫
などを殺すことが多く、自然の生態系を破壊してしまう
という点で、自然に対して非常に有害なことであり、こ
れに変わる方法の開発が望まれているのが現状である。[0005] A typical example of damage to trees is the death of pine trees caused by pine wood nematodes. Once damage has occurred, it is effective other than cutting down the pine trees that have caused pine blight and incinerating them. Are unable to find any appropriate countermeasures. The method of controlling pine wilt by spraying chemicals is very harmful to nature in that it often kills harmless insects and destroys natural ecosystems. It is the current situation that the development of is desired.
【０００６】[0006]
【発明が解決しようとする課題】本発明は上記事情に鑑
みてなされたもので、その課題は、環境汚染が少なく、
生態系に調和し、植物寄生線虫などによる土壌病害、森
林の被害に対する防除効果の安定性、持続性および広域
性に優れた線虫の活性抑制剤を提供することにある。SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and has as its object to reduce environmental pollution.
An object of the present invention is to provide a nematode activity inhibitor which is in harmony with the ecosystem and has excellent stability, sustainability, and wide area of the control effect on soil diseases and forest damage caused by plant parasitic nematodes.
【０００７】[0007]
【課題を解決するための手段】上記課題を解決するため
に、本発明者は、土壌病害、森林の樹木病害の発生に関
与する線虫の活性を抑制する方法について種々検討した
結果、コーヒー粕発酵物および／またはコーヒー粕抽出
液が線虫の活性抑制効果を有することを見出し、本発明
をなすに至った。Means for Solving the Problems In order to solve the above problems, the present inventors have conducted various studies on methods for suppressing the activity of nematodes involved in the occurrence of soil diseases and forest tree diseases. The present inventors have found that a fermented product and / or a coffee grounds extract have a nematode activity suppressing effect, and have accomplished the present invention.
【０００８】すなわち、本発明は、コーヒー粕抽出液お
よび／またはコーヒー粕発酵物を含有することを特徴と
する線虫の活性抑制剤に関する。本発明によれば、多く
の野菜、花卉、芝草、果樹および樹木で報告されてい
る、各種の植物寄生性線虫に起因する土壌病害、森林の
樹木病害が有効に防除できる。That is, the present invention relates to a nematode activity inhibitor comprising a coffee grounds extract and / or a coffee grounds fermented product. According to the present invention, it is possible to effectively control soil diseases and forest tree diseases caused by various plant parasitic nematodes, which have been reported in many vegetables, flowers, turfgrass, fruit trees, and trees.
【０００９】以下、本発明を詳細に説明する。本発明に
いう線虫の活性抑制剤とは、コーヒー粕発酵物および／
またはコーヒー粕抽出液を主成分として含有するもので
ある。コーヒー粕発酵物は、コーヒー粕を野積堆積法に
より発酵させて堆肥化したり、近畿大学環境科学研究所
研究報告、第１８号の「コーヒー粕の肥料化による研
究」（１３３〜１４９頁）に報告されたように、堆肥化
の前処理として鶏糞、もみがら、米糠を混合し、Ｃ／Ｎ
を１０〜２０に低下させた後、これに生化学的処理を加
え、腐食質系土壌改良剤とする方法、あるいは生のコー
ヒー粕に植種したコーヒー粕肥料を加え、好気性発酵さ
せる際に、材料中のＣ／Ｎが５〜２０となるように硫酸
アンモニウムを添加して、発酵過程で生成された生育障
害の原因となる有機酸を速やかに分解する、好気方法に
よって得られる。Hereinafter, the present invention will be described in detail. The nematode activity inhibitor referred to in the present invention is a coffee grounds fermented product and / or
Alternatively, it contains a coffee grounds extract as a main component. Fermented coffee grounds are composted by fermenting coffee grounds by the Nozumi sedimentation method, and are reported in Kinki University Institute for Environmental Science Research Report, No. 18, "Study on Making Coffee Grounds into Fertilizer" (pp. 133-149). As a pretreatment for composting, chicken manure, rice husk, rice bran were mixed, and C / N
After reducing to 10-20, adding a biochemical treatment to this, a method as a corrosive soil improver, or adding a coffee grounds fertilizer planted in raw coffee grounds, when aerobic fermentation An ammonium salt is added so that the C / N in the material is 5 to 20 to quickly decompose the organic acid generated in the fermentation process and causing the growth impairment.
【００１０】コーヒー粕抽出液は、コーヒー粕１００部
に対して５０〜１０００部の水（室温〜１００℃）を加
えてミキサーにかけた後ろ過する等の方法で得られる。
この際、抽出効率をあげるためエーテル、ケトン、アル
コール等の有機溶剤を、使用する水に対して１０％の範
囲で使用する事は可能である。本発明の線虫の活性抑制
剤は、上記の主成分の他に、本発明の効果を阻害しない
範囲で、その他の成分を含んでいてもよい。具体的に
は、たとえば、米糠、鶏糞、廃糖蜜、モミガラ、おか
ら、麦芽エキス、高級脂肪酸、堆肥、動植物腐植、アミ
ノ酸、ブドウ糖、キチンなどの有機物、あるいは硫安、
尿素、塩化カリ、過燐酸石灰などの肥料成分、マンガ
ン、亜鉛、コバルト、鉄、ヨード、珪素、苦土、ホウ
素、フッ素、モリブデンなどに代表される微量ミネラル
などがあげられる。これらの成分はコーヒー粕抽出液あ
るいはコーヒー粕発酵物を施用する際に混合して用いた
り、コーヒー粕発酵物を製造する際に工程の途中で添加
したりして用いることが出来る。[0010] The coffee grounds extract is obtained by adding 50 to 1000 parts of water (room temperature to 100 ° C) to 100 parts of coffee grounds, filtering the mixture, followed by filtration.
At this time, in order to increase the extraction efficiency, it is possible to use an organic solvent such as ether, ketone or alcohol in a range of 10% with respect to the water used. The nematode activity inhibitor of the present invention may contain, in addition to the above main components, other components as long as the effects of the present invention are not impaired. Specifically, for example, rice bran, chicken dung, molasses, peach, okara, malt extract, higher fatty acids, compost, animal and plant humus, amino acids, glucose, organic substances such as chitin, or ammonium sulfate,
Examples include fertilizer components such as urea, potassium chloride, and lime superphosphate, and trace minerals such as manganese, zinc, cobalt, iron, iodine, silicon, magnesium, boron, fluorine, and molybdenum. These components can be used as a mixture when applying a coffee grounds extract or a coffee grounds fermented product, or can be added during the process of producing a coffee grounds fermented product.
【００１１】本発明の線虫活性抑制剤の形態としては、
コーヒー粕堆肥あるいはコーヒー粕堆肥をペレット状に
したもの、コーヒー粕抽出液を無機系のバーミキュライ
ト、ゼオライト、珪藻土、炭カル、石灰岩、火山灰、赤
土、活性けい酸、金属酸化物、多孔質鉱物、ソフトセラ
ミックス及び有機系の腐植、動物質石灰などの多孔質担
体に吸着・安定化させたもの等が挙げられる。このう
ち、効果の持続性あるいは有効成分の徐放性の点から
は、コーヒー粕堆肥あるいはコーヒー粕抽出液を多孔質
体に吸着・安定化させた形態のものを用いるのが好まし
い。The form of the nematode activity inhibitor of the present invention includes
Coffee grounds compost or coffee grounds compost pelletized, coffee grounds extract is made of inorganic vermiculite, zeolite, diatomaceous earth, charcoal, limestone, volcanic ash, red earth, activated silica, metal oxide, porous mineral, soft mineral Examples thereof include those adsorbed and stabilized on a porous carrier such as ceramics and organic humus and animal lime. Among them, it is preferable to use a form obtained by adsorbing and stabilizing a coffee grounds compost or a coffee grounds extract on a porous material from the viewpoint of the sustained effect and the sustained release of the active ingredient.
【００１２】また、線虫による被害が発生してしまい、
その被害の程度をできるだけ最少にくい止めるために
は、即効性が要求される点から、コーヒー粕抽出液を用
いるのが好ましい。更に樹木の樹幹に注入して、病原体
である線虫の感染を防ぐ様な場合には当然の事ながら、
コーヒー粕抽出液を用いるのが有効である。本発明の線
虫活性抑制剤は、たとえば、上記の形態をとることがで
きるが、多孔質体に吸着・安定化させる場合は、抽出液
１００部に対して、前記した多孔質体５００〜５０００
ｃｍ3に吸着させることで作製できる。In addition, damage by nematodes occurs,
In order to minimize the extent of the damage as much as possible, it is preferable to use a coffee grounds extract from the viewpoint that immediate effect is required. In addition, if it is injected into the trunk of a tree to prevent the infection of the pathogen, nematode, of course,
It is effective to use a coffee cake extract. The nematode activity inhibitor of the present invention can take, for example, the above-described form. When adsorbing and stabilizing on a porous body, the above-described porous body 500 to 5000 is used with respect to 100 parts of the extract.
It can be produced by adsorbing to cm 3 .
【００１３】本発明に言う植物寄生性線虫とは、土壌に
生息する土壌病害線虫および木材及び木材の組織に寄生
し害を及ぼす線虫をさし、具体的には、サツマイモネコ
ブ線虫、ジャワネコブ線虫、アレナリアネコブ線虫、キ
タネコブ線虫、リンゴネコブ線虫等に代表されるネコブ
線虫類、またキタネグサレ線虫、ミナミネグサレ線虫、
ムギネグサレ線虫等に代表されるネグサレ線虫類、さら
にダイズシスト線虫、ジャガイモシスト線虫、イネシス
ト線虫等に代表されるシスト線虫類、そしてミカンネモ
グリ線虫、イネネモグリ線虫等に代表されるネモグリ線
虫類、ミカンネ線虫、ニセフクロウ線虫等に代表される
フクロウ線虫類、ピン線虫類、マツノザイ線虫等が挙げ
られる。The term "plant parasitic nematode" as used in the present invention refers to a nematode that infests soil and a nematode that infests and damages wood and wood tissue. Specifically, a sweet potato nematode , Nekobu nematodes, Arenaria Nekobu nematodes, Kitanekobu nematodes, Nekobu nematodes represented by Apple Nekobu nematodes, etc., Kitanegusare nematodes, Minaminexare nematodes,
Negusare nematodes typified by Mugi Negusare nematodes, etc., and cyst nematodes typified by soybean cyst nematodes, potato cyst nematodes, rice cyst nematodes, etc .; Owl nematodes, such as nematodes, Ranchus nematodes, and Nise Owl nematodes, pin nematodes, pine worms, and the like.
【００１４】本発明の線虫活性抑制剤を用いて、圃場で
の線虫に起因する土壌病害を抑制する方法としては、例
えば、本発明の線虫活性抑制剤を、播種または定植前の
土壌に混和したり、植物の苗根部または土壌に処理する
ことにより防除することができる。具体的には、植物苗
根部浸漬処理、植物苗根部粉衣処理、株元灌注処理、土
壌混和処理などが挙げられる。As a method for suppressing a soil disease caused by a nematode in a field by using the nematode activity inhibitor of the present invention, for example, the method of controlling the nematode activity inhibitor of the present invention in a soil before sowing or planting is used. Or by treating the seedlings of plants or soil. Specific examples include a plant root portion immersion process, a plant root portion dressing process, a plant irrigation process, a soil mixing process, and the like.
【００１５】植物苗根部浸漬処理は、コーヒー粕抽出液
に植物根部を０．５時間〜２４時間程度浸漬するのが好
ましい。植物根部粉衣処理は植物根部にコーヒー粕抽出
液の吸着担体あるいはカプセルを粉衣することで実施で
きる。株元灌注処理は、植物苗根部を土壌に定植する時
コーヒー粕抽出液を株元に灌注することで実施出来る。
その際の散布量の目安としては、コーヒー粕抽出液とし
て１ｇ／ｍ2 〜１００ｇ／ｍ2 となる様に散布するのが
好ましい。実際の散布に際しては、抽出原液をそのまま
散布すると植物によっては成育に害を受ける物もあるの
で、水で１００倍から５０００倍程度に希釈して用いる
必要がある。In the plant seedling immersion treatment, the plant root is preferably immersed in the coffee grounds extract for about 0.5 to 24 hours. The plant root dressing treatment can be carried out by coating the plant root with an adsorbent or a capsule of the coffee grounds extract. The root irrigation treatment can be carried out by irrigating a coffee grounds extract into the root of the plant when planting the seedlings of the plant in soil.
As a guideline the scattering amount of time, preferably sprayed as a 1g / m 2 ~100g / m 2 as coffee grounds extract. In actual spraying, some plants may be harmful to the growth if the undiluted extract solution is sprayed as it is, so it is necessary to dilute it with water about 100- to 5000-fold before use.
【００１６】土壌混和処理は、コーヒー粕堆肥あるいは
コーヒー粕抽出液を多孔質担体に担持させたものを、播
種前に土壌に混和しても、育苗開始時に発根用土に混合
したり、定植前に定植用土壌に混和したり、あるいは全
ての時期に処理することも効果を高めるためには好まし
い。土壌混和に際しても、混和量の目安は、コーヒー粕
堆肥、あるいはコーヒー粕抽出液担持体として２００ｇ
／ｍ2〜１００００ｇ／ｍ2が好ましい。[0016] In the soil admixture treatment, the coffee grounds compost or the coffee grounds extract supported on a porous carrier is mixed with the soil before sowing, but is mixed with the soil for rooting at the start of seedling or before planting. It is also preferable to mix the soil with the planting soil or to treat it at all times in order to enhance the effect. In the case of soil mixing, the standard of the mixing amount is 200 g of coffee grounds compost or coffee grounds extract liquid carrier.
/ M 2 to 10,000 g / m 2 are preferred.
【００１７】[0017]
【発明の実施の形態】以下、本発明を実施例によってさ
らに詳細に説明するが、本発明はこれらの例によって何
等限定されるものではない。実施例中に用いられる線虫
活性抑制剤の調整方法および各測定値の測定方法は、下
記の通りである。DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples. The method of adjusting the nematode activity inhibitor used in the examples and the method of measuring each measured value are as follows.
【００１８】１）コーヒー粕抽出液の調整：コーヒー粕
５０ｇを蒸留水２００ｍｌとともにミキサーにかけ、ガ
ーゼでろ過した後、８０００ｒｐｍで２０分遠心分離
し、得られた上澄みを抽出液として用いた。 ２）線虫分散液の調整：ベールマン法により、土壌から
分離・捕集した、チャネグサレ線虫、キタネグサレ線
虫、マツノザイ線虫線虫を、それぞれ水の入った容器に
一定量入れて、水分散液を作成した。1) Preparation of coffee grounds extract: 50 g of coffee grounds was put into a mixer together with 200 ml of distilled water, filtered with gauze, centrifuged at 8000 rpm for 20 minutes, and the obtained supernatant was used as an extract. 2) Preparation of nematode dispersion: A certain amount of each of C. elegans, C. elegans, and P. elegans isolated and collected from the soil by the Berman method is placed in a container containing water and dispersed in water. A liquid was made.
【００１９】３）線虫死亡率の測定：タテ×ヨコ×深さ
＝４０ｍｍ×８０ｍｍ×６ｍｍのプレパラートの中央
に、直径３０ｍｍ、深さ５ｍｍの円柱状の計数室（容積
３．５ｍｌ）を作り、計数用プレパラートを作成した。
そこに、線虫分散液２．５ｍｌと試験液１．０ｍｌを満
たして、カバーグラスで覆いをし、２５℃、６０％ＲＨ
の恒温恒湿の部屋に、光が当たらないようにして静置し
た。生線虫数と死亡線虫数の測定は、経時的に倒立顕微
鏡を用いて測定し、以下の式により、線虫死亡率を計算
で求めた。測定から測定の間はカバ−グラスを少しずら
して空気が不足しないようにした。3) Measurement of nematode mortality: A cylindrical counting chamber (volume: 3.5 ml) having a diameter of 30 mm and a depth of 5 mm was formed in the center of a preparation having a size of vertical x horizontal x depth = 40 mm x 80 mm x 6 mm. A preparation for counting was prepared.
There, 2.5 ml of the nematode dispersion and 1.0 ml of the test solution were filled, covered with a cover glass, and heated at 25 ° C. and 60% RH.
In a room of constant temperature and humidity with no light. The numbers of live and dead nematodes were measured over time using an inverted microscope, and the nematode mortality was calculated by the following formula. The cover glass was slightly displaced from measurement to measurement so that there was no shortage of air.
【００２０】線虫死亡率（％）＝〔死亡線虫数（頭）／
生線虫数（頭）〕×１００ ４）土壌中の病害線虫の測定（ベールマン法）：植物根
圏の土壌約１００ｇを採取し、直径１０ｃｍのロートの
端にゴム管をつないでピンチコックで止め、ロートに満
たした水の中にティッシュペーパーに包んだ２０ｇの土
を静かに沈め、２５℃の恒温室で２４時間静置すると、
土壌中の線虫は紙の目を通り遊出し、ゴム管内に沈降す
る。ピンチコックを開いてビーカーに集め、内容積３ｃ
ｃで、底部に罫線のある、円柱状の液ダマリを有するプ
レパラートに、線虫を分離した液を入れ、倒立顕微鏡に
て、病害線虫の数を数える。土壌よりの線虫分離は２回
反復し、データーとしては２回の計数の合計、つまり４
０ｇ土中の線虫数として表示した。Nematode mortality (%) = [Number of dead nematodes (head) /
Number of live nematodes (heads)] × 100 4) Measurement of disease nematodes in soil (Berman method): Approximately 100 g of soil in the plant rhizosphere was collected, and a rubber tube was connected to the end of a funnel having a diameter of 10 cm to pinch cock. , Gently submerge 20 g of tissue wrapped in tissue paper in the water filled with the funnel, and let stand in a constant temperature room at 25 ° C for 24 hours.
Nematodes in the soil migrate through the eyes of the paper and settle in the rubber tube. Open the pinch cock and collect in a beaker, 3c internal volume
In c, the liquid obtained by separating the nematodes is placed in a preparation having a columnar liquid sump with a ruled line at the bottom, and the number of disease nematodes is counted using an inverted microscope. Separation of nematodes from soil was repeated twice, and the data was the sum of the two counts, ie, 4
It was expressed as the number of nematodes in 0 g soil.
【００２１】[0021]
【実施例１】チャネグサレ線虫の水分散液１．５ｍｌ
を、計数用プレパラートの計数室に入れた。コーヒー粕
抽出液１ｍｌを添加して、良く混合した後、２時間、４
時間、６時間、２４時間経過した時点で線虫死亡率を測
定した。その結果を表１に示す。[Example 1] 1.5 ml of an aqueous dispersion of C. elegans
Was placed in a counting chamber of a counting slide. After adding 1 ml of coffee cake extract and mixing well,
After 6 hours and 24 hours, the nematode mortality was measured. Table 1 shows the results.
【００２２】[0022]
【実施例２】キタネグサレ線虫の水分散液１．５ｍｌ
を、計数用プレパラートの計数室に入れた後、コーヒー
粕抽出液液１ｍｌを添加して、良く混合した後、実施例
１と同様に、経時的に線虫死亡率を測定した。その結果
を表１に示す。[Example 2] 1.5 ml of an aqueous dispersion of Kita Negusare nematodes
Was placed in a counting chamber of a preparation for counting, 1 ml of a coffee grounds extract was added, and the mixture was mixed well. Then, the nematode mortality was measured over time as in Example 1. Table 1 shows the results.
【００２３】[0023]
【実施例３】キタネコブ線虫を用いた以外は、実施例１
と同様にして、経時的に線虫死亡率を測定した。その結
果を表１に示す。Example 3 Example 1 was repeated except that Kitanekob nematode was used.
The nematode mortality was measured over time in the same manner as in the above. Table 1 shows the results.
【００２４】[0024]
【実施例４】マツノザイ線虫の水分散液１.５ｍｌを、
計数用プレパラートの計数室に入れた。コーヒー粕抽出
液１ｍｌを添加して、良く混合した後、２時間、４時
間、６時間、２４時間経過した時点で線虫死亡率を測定
した。Example 4 1.5 ml of an aqueous dispersion of Matsunosai nematodes was
The slides were placed in a counting chamber of a preparation for counting. After 1 ml of the coffee cake extract was added and mixed well, the nematode mortality was measured at 2 hours, 4 hours, 6 hours, and 24 hours.
【００２５】[0025]
【比較例１】線虫分散液としてチャネグサレ線虫を用
い、該分散液に用いたと同じ水道水を用いて、実施例１
と同様にして、経時的に線虫死亡率を測定した。その結
果を表１に示す。[Comparative Example 1] A nematode dispersion was used as a nematode dispersion, and the same tap water as used in the dispersion was used.
The nematode mortality was measured over time in the same manner as in the above. Table 1 shows the results.
【００２６】[0026]
【比較例２】線虫分散液として、キタネグサレ線虫を用
いた以外は、比較例１と同様にして経時的に線虫死亡率
を測定した。その結果を表１に示す。Comparative Example 2 The nematode mortality was measured over time in the same manner as in Comparative Example 1 except that the nematode dispersal liquid was C. elegans. Table 1 shows the results.
【００２７】[0027]
【比較例３】線虫分散液として、キタネコブ線虫を用い
た以外は、比較例１と同様にして、経時的に線虫死亡率
を測定した。その結果を表１に示す。Comparative Example 3 The nematode mortality was measured over time in the same manner as in Comparative Example 1 except that Kitanekobu nematode was used as the nematode dispersion. Table 1 shows the results.
【００２８】[0028]
【比較例４】線虫分散液として、マツノザイ線虫を用い
た以外は、比較例１と同様にして、経時的に線虫死亡率
を測定した。その結果を表１に示す。Comparative Example 4 The nematode mortality was measured over time in the same manner as in Comparative Example 1 except that Matsunozai nematodes were used as the nematode dispersion. Table 1 shows the results.
【００２９】[0029]
【表１】 [Table 1]
【００３０】[0030]
【実施例５〜７及び比較例５、６】キュウリの連作障害
が問題になっている、静岡県沼津市の圃場を用いて評価
実験を行った。評価の手順は次の如くである。 １）圃場に、油粕を２９５ｇ／ｍ2 、発酵鶏糞４５８ｇ
／ｍ2 、発酵牛糞４３２２ｇ／ｍ2 、くみあいジシアン
有機入り化成；特８０６を３４ｇ／ｍ2 、苦土石灰２５
１ｇ／ｍ2 を施用し、キュウリの未床苗（夏すずみ）を
８０本定植した。Examples 5 to 7 and Comparative Examples 5 and 6 An evaluation experiment was conducted using a field in Numazu City, Shizuoka Prefecture, in which continuous cropping failure of cucumber was a problem. The evaluation procedure is as follows. 1) In the field, 295 g / m 2 of oil cake and 458 g of fermented chicken manure
/ M 2, fermented cow dung 4322g / m 2, union cyanogen organic-containing chemical conversion; a Japanese 806 34g / m 2, dolomite 25
1 g / m 2 was applied, and 80 unbedded cucumber seedlings (Natsuno Suzumi) were planted regularly.
【００３１】２）収穫終了後、株を抜き取り、整地した
後、圃場のネコブセンチュウ数を測定した。 ３）圃場を２ｍ×５ｍ＝１０ｍ2 毎に区切り、各区をア
クリル板を用いて、深さ１５ｃｍ、高さ２０ｃｍに囲っ
た。 ４）各区に１と同様にして、元肥を施用した後、表２に
示す如くの割合にて、コーヒー粕堆肥（（株）緑産製
「肥効素ドライ」）を施用した。2) After completion of the harvest, the plants were extracted, the ground leveled, and the number of root-knot nematodes in the field was measured. 3) The field was divided into 2 m × 5 m = 10 m 2 sections, and each section was surrounded by an acrylic plate at a depth of 15 cm and a height of 20 cm. 4) After applying the original manure to each section in the same manner as in 1, the coffee lees compost (“Hiengen Dry” manufactured by Ryokusan Co., Ltd.) was applied at the ratio shown in Table 2.
【００３２】５）施用後、１週間放置した後、圃場のネ
コブセンチュウを測定した後、キュウリの未床苗（夏す
ずみ）を各区に１６本づつ定植した。 ６）定植後、１ケ月後に苗の成育状態を観察し、ネコブ
線虫数と枯死率を求めた。その結果を表２に示す。5) After application, the plants were allowed to stand for one week, and after measuring the root-knot nematodes in the field, 16 uncultivated cucumber seedlings (Natsu Suzumi) were planted in each section. 6) One month after planting, the state of growth of the seedlings was observed, and the number of caterpillar nematodes and the death rate were determined. Table 2 shows the results.
【００３３】[0033]
【表２】 [Table 2]
【００３４】[0034]
【発明の効果】本発明における、コーヒー粕堆肥あるい
はコーヒー粕抽出液を用いることにより、環境や作業者
に及ぼす影響の極めて少ない、毒性の低い線虫活性抑制
剤が得られる。According to the present invention, by using the coffee grounds compost or the coffee grounds extract, it is possible to obtain a low toxic nematode activity inhibitor which has very little effect on the environment and workers.
Claims (1)
ー粕発酵物を含有することを特徴とする線虫の活性抑制
剤。A nematode activity inhibitor comprising a coffee grounds extract and / or a coffee grounds fermented product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26020797A JPH1192321A (en) | 1997-09-25 | 1997-09-25 | Activity inhibitor of nematode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26020797A JPH1192321A (en) | 1997-09-25 | 1997-09-25 | Activity inhibitor of nematode |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH1192321A true JPH1192321A (en) | 1999-04-06 |
Family
ID=17344838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP26020797A Pending JPH1192321A (en) | 1997-09-25 | 1997-09-25 | Activity inhibitor of nematode |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH1192321A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU767628B3 (en) * | 1999-07-14 | 2001-02-05 | Palmrow Pty Ltd | A nematicide composition |
| KR100483060B1 (en) * | 2002-09-18 | 2005-04-15 | (주)오비티 | Anti-nematode agents containing Jack-Bean(Canavalia sp.) |
| JP4630434B2 (en) * | 2000-08-31 | 2011-02-09 | サンケイ化学株式会社 | Agricultural / horticultural suspension in water |
| JP2012232950A (en) * | 2011-05-09 | 2012-11-29 | Panefuri Kogyo Kk | Nematode-controlling agent |
| JP2017132665A (en) * | 2016-01-29 | 2017-08-03 | 株式会社丹勝 | Pine withering inhibitor and improver, and method for inhibiting pine withering |
| KR20210125203A (en) * | 2020-04-08 | 2021-10-18 | 정원철 | Antibacterial composition containing coffee waste extract |
-
1997
- 1997-09-25 JP JP26020797A patent/JPH1192321A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU767628B3 (en) * | 1999-07-14 | 2001-02-05 | Palmrow Pty Ltd | A nematicide composition |
| JP4630434B2 (en) * | 2000-08-31 | 2011-02-09 | サンケイ化学株式会社 | Agricultural / horticultural suspension in water |
| KR100483060B1 (en) * | 2002-09-18 | 2005-04-15 | (주)오비티 | Anti-nematode agents containing Jack-Bean(Canavalia sp.) |
| JP2012232950A (en) * | 2011-05-09 | 2012-11-29 | Panefuri Kogyo Kk | Nematode-controlling agent |
| JP2017132665A (en) * | 2016-01-29 | 2017-08-03 | 株式会社丹勝 | Pine withering inhibitor and improver, and method for inhibiting pine withering |
| KR20210125203A (en) * | 2020-04-08 | 2021-10-18 | 정원철 | Antibacterial composition containing coffee waste extract |
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