JPH11171722A - Preparation for external use for skin - Google Patents
Preparation for external use for skinInfo
- Publication number
- JPH11171722A JPH11171722A JP9356157A JP35615797A JPH11171722A JP H11171722 A JPH11171722 A JP H11171722A JP 9356157 A JP9356157 A JP 9356157A JP 35615797 A JP35615797 A JP 35615797A JP H11171722 A JPH11171722 A JP H11171722A
- Authority
- JP
- Japan
- Prior art keywords
- skin
- extract
- birch
- aging
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、カバノキ科、ハン
ノキ科属植物のポリフェノール類およびその配糖体を有
効成分とする、しわ、肌荒れの改善、しみ、くすみの軽
減、さらには肌の潤いや弾力性を改善する事によって優
れた効果を発揮する皮膚外用剤に関する。BACKGROUND OF THE INVENTION The present invention relates to the improvement of wrinkles and rough skin, the reduction of spots and dullness, and the improvement of skin moisturizing, comprising as an active ingredient polyphenols of the plants of the birch family and Alderaceae. The present invention relates to an external preparation for skin which exhibits excellent effects by improving elasticity.
【０００２】[0002]
【従来技術と問題点】シワやシミの発生、またくすみや
肌荒れは代表的な皮膚の老徴である。その原因はいまだ
完全に解明されてはいないが、現段階では、加齢に伴な
う表皮基底細胞や真皮繊維芽細胞の***能の低下と機能
の低下、そしてその事によって生じる皮膚全体の萎縮、
さらには表皮細胞自身が作り出す天然保湿成分量の減少
などが、皮膚の柔軟性や弾力性の低下をもたらし、結果
として肌荒れやシワなどの要因となると考えられる。ま
た近年では、このような加齢による老化現象とともに、
紫外線等、光線などの外的な要因によるダメージが、肌
の老化現象の促進をうながす事が明らかとなった。これ
らは、通常の老化とは異なる、いわゆる光老化として定
義付けられている。この光老化は、解剖学、形態学的に
通常の加齢による老化とは異なる現象であると考えられ
ているが、現段階ではその詳細な機構は解明されていな
い。しかし加齢による老化現象と同様、結果として、や
はり肌に、とくに光に暴露されている顔や首、腕など
に、シワやシミ、くすみなどの老化の徴候を生じる事が
特徴である。さらに加齢による老化および光老化の進行
には、ともにフリーラジカルや活性酸素が何らかの形で
関与している事が明らかとなってきた。すなわち、通
常、これらのフリーラジカルや活性酸素は、カタラーゼ
やグルタチオンペルオキシターゼ、スーパーオキサイド
ジスムターゼのような酵素、さらには生体内に存在する
ビタミンＣやＥなどにより消去されるが、加齢、さらに
は紫外線等によるこれら抗酸化酵素や成分の減少や機能
低下が、結果として皮膚内に過剰の活性酸素や過酸化脂
質を生じさせ、組織傷害、細胞の機能低下などをもたら
し、その結果、肌にシミ、シワ、くすみや乾燥などの老
化の徴候がもたらされると考えられる。2. Description of the Related Art The occurrence of wrinkles and spots, dullness and rough skin are typical signs of skin aging. Although the cause has not yet been completely elucidated, at this stage, the age-related decrease in the division ability and function of epidermal basal cells and dermal fibroblasts, and the resulting atrophy of the entire skin ,
Furthermore, a decrease in the amount of natural moisturizing components produced by the epidermal cells themselves may lead to a decrease in the flexibility and elasticity of the skin, resulting in factors such as rough skin and wrinkles. In recent years, along with such aging phenomenon due to aging,
It has been found that damage caused by external factors such as light rays, such as ultraviolet rays, promotes the aging phenomenon of the skin. These are defined as so-called light aging, which is different from normal aging. This photoaging is considered to be a phenomenon different from normal aging due to anatomy and morphology, but its detailed mechanism has not been elucidated at this stage. However, similar to the aging phenomenon due to aging, as a result, signs of aging such as wrinkles, spots, dullness, etc. are also characteristic on the skin, especially on the face, neck, and arms exposed to light. Furthermore, it has become clear that both free radicals and active oxygen are involved in the progress of aging and photoaging due to aging. That is, these free radicals and active oxygen are usually eliminated by enzymes such as catalase, glutathione peroxidase, and superoxide dismutase, and vitamins C and E existing in the living body. As a result, the decrease or decrease in the function of these antioxidant enzymes and components causes excessive active oxygen and lipid peroxide in the skin, resulting in tissue damage, decreased cell function, etc. It is believed that it will cause signs of aging such as wrinkles, dullness and dryness.
【０００３】以上のような現状に鑑み、従来より肌の老
化防止あるいはその症状緩和を目的として、様々な素材
を配合した化粧料が提案されてきた。しかしながらいま
だ十分な効果が得られていない。例えば、ビタミン類や
プラセンタエキスのような細胞賦活作用を持つ成分を化
粧料に配合する試みがなされてきたが、シワや肌荒れ、
シミ、くすみなどの肌の老化の徴候を十分に抑える事は
できなかった。 また、種々の抗酸化剤の化粧料への配
合も検討されいるが、やはり十分な効果が得られていな
いのが現状である。[0003] In view of the above situation, cosmetics containing various materials have been proposed for the purpose of preventing skin aging or alleviating the symptoms thereof. However, sufficient effects have not yet been obtained. For example, attempts have been made to incorporate components having a cell-activating effect, such as vitamins and placenta extract, into cosmetics, but wrinkles, rough skin,
The signs of skin aging, such as spots and dullness, could not be sufficiently suppressed. In addition, blending of various antioxidants into cosmetics has been studied, but at present it has not been able to obtain a sufficient effect.
【０００４】[0004]
【発明が解決しようとする課題】カバノキ科のカバノキ
属、ハンノキ科属植物の抽出物は、従来から肌の老化現
象全般を効果的に改善する作用が知られていた（特開平
６−２６３６２７号公報）。本発明者らは、さらに改善
度をたかめることを目的に、鋭意検討した。An extract of a birch or alder plant of the birch family has been known to have an effect of effectively improving the overall skin aging phenomenon (Japanese Patent Application Laid-Open No. 6-263627). Gazette). The present inventors have conducted intensive studies for the purpose of further enhancing the degree of improvement.
【０００５】[0005]
【問題を解決するための手段】そして、ポリフェノール
類およびその配糖体を３０％以上含有するカバノキ科の
カバノキ属、ハンノキ属植物の抽出物が、シワの発生や
肌荒れ、シミ、くすみなどの肌の老化現象全般を効果的
に改善する作用を有する事を見い出し、本発明を完成さ
せるに至った。すなわち、本発明は、カバノキ科、ハン
ノキ科属植物のポリフェノール類およびその配糖体を３
０％以上含有する抽出物を配合することを特徴とする皮
膚外用剤を提供するものである。[Means for Solving the Problems] Extracts of birch plants and alder plants of the birch family containing 30% or more of polyphenols and glycosides thereof are used for producing wrinkles, rough skin, spots, dullness, etc. Have been found to have an effect of effectively improving the aging phenomenon in general, and have completed the present invention. That is, the present invention relates to polyphenols and their glycosides of birch and alder plants.
It is intended to provide a skin external preparation characterized by incorporating an extract containing 0% or more.
【０００６】[0006]
【発明の実施の形態】本発明で用いるポリフェノ−ル類
およびその配糖体を含有する抽出物は、カバノキ科カバ
ノキ属あるいは、ハンノキ属の植物から製造される。カ
バノキ科カバノキ属としては、シラカバ（Ｂｅｔｕｌａ
ｐｌａｔｙｐｈｙｌｌａ Ｓｕｋａｔｃｈｅｖ ｖａ
ｒ．ｊａｐｏｎｉｃａ Ｈａｒａ）、ダケカンバ（Ｂｅ
ｔｕｌａ Ｅｒｍａｎｉ Ｃｈａｍ．）、ジゾウカンバ
（Ｂｅｔｕｌａ ｇｌｏｂｉｓｐｉｃａ Ｓｉｒａ
ｉ）、ヤエガワカンバ（Ｂｅｔｕｌａ ｄａｖｕｒｉｃ
ａＰａｌｌ．）、マカンバ（Ｂｅｔｕｌａ ｎｉｋｏｅ
ｎｓｉｓ Ｋｏｉｄｚ．）、ウダイカンバ（Ｂｅｔｕｌ
ａ Ｍａｘｉｍｏｗｉｃｚｉａｎａ Ｒｅｇｅｌ）、ア
ズサ（Ｂｅｔｕｌａ ｇｒｏｓｓａ Ｓｉｅｂ．ｅｔ
Ｚｕｃｃ． ｖａｒ．ｕｌｍｉｆｏｌｉａ Ｍａｋｉｎ
ｏ）、ミズメ（Ｂｅｔｕｌａ ｇｒｏｓｓａ Ｓｉｅ
ｂ．ｅｔ Ｚｕｃｃ．）、ウラジロカンバ（Ｂｅｔｕｌ
ａ ｃｏｒｙｌｉｆｏｌｉａ Ｒｅｇｅｌ ｅｔ Ｍａ
ｘｉｍ．）、オノオレ（ＢｅｔｕｌａＳｃｈｍｉｄｔｉ
ｉ Ｒｅｇｅｌ）、エゾシラカンバ（Ｂｅｔｕｌａ ｐ
ｌａｔｙｐｈｙｌｌａ Ｓｕｋ．）、シダレカンバ（Ｂ
ｅｔｕｌａ ｐｅｎｄｕｌａＲｏｔｈ）が例示でき、ハ
ンノキ属の植物としては、ハンノキ（Ａｌｎｕｓｊａｐ
ｏｎｉｃａ Ｓｔｅｕｄ．）、ヤマハンノキ（Ａｌｎｕ
ｓ ｈｉｒｓｕｔａ Ｔｕｒｃｚ．）、ヤハズハンノキ
（Ａｌｎｕｓ Ｍａｔｓｕｍｕｒａｅ Ｃａｌｌ．）、
カワラハンノキ（Ａｌｎｕｓ ｓｅｒｒｕｌａｔｏｉｄ
ｅｓ Ｃａｌｌ．）、ミヤマハンノキ（Ａｌｎｕｓ Ｍ
ａｘｉｍｏｗｉｃｚｉｉ Ｃａｌｌ．）、ヤシャブシ
（Ａｌｎｕｓ ｆｉｒｍａ Ｓｉｅｂ．ｅｔ Ｚｕｃ
ｃ．）、ヒメヤシャブシ（Ａｌｎｕｓ ｐｅｎｄｕｌａ
Ｍａｔｓｕｍ．）が例示できる。これら植物の、木
部、樹皮、根、葉などのいずれを原料とすることができ
るが、特にシラカバの樹皮が好ましい。BEST MODE FOR CARRYING OUT THE INVENTION The polyphenols and their glycoside-containing extracts used in the present invention are produced from plants of the birch family Birch or Alder. Birch species include birch species (Betula).
Platyphylla Sukatchev va
r. japonica Hara, Bamboo birch (Be)
tula Ermani Cham. ), Betula globispica Sira
i), Betula daburic
aPall. ), Birch (Betula nikoe)
nsis Koidz. ), Udai kamba (Betul)
a Maximowicziana Regel, Azusa (Betula grossa Sieb. et.
Zucc. var. ulmifolia Makin
o), watermelon (Betula grossa Sie)
b. et Zucc. ), Betula (Betul)
a corylifolia Regel et Ma
xim. ), Onoole (BetulaSchmidti)
i Regel), Betula birch (Betula p)
latephylla Suk. ), Weeping birch (B
etula pendulaRoth). Examples of plants of the genus Alder include Alnusjap.
onica Steud. ), Alder (Alnu)
siruta Turcz. ), Alnus alder (Alnus Matsumurae Call.),
Japanese Alder (Alnus serrulatoid)
es Call. ), Alder M
aximowiczii Call. ), Yashabushi (Alnus farm Sieb. Et Zuc)
c. ), Himeya shabushi (Alnus pendula)
Matsum. ) Can be exemplified. Any of the xylem, bark, roots, leaves and the like of these plants can be used as a raw material, but the bark of birch is particularly preferred.
【０００７】本発明に用いるポリフェノ−ル類およびそ
の配糖体を含有する抽出物は、上記の植物より例えば、
水、エタノール、プロパノール、ブタノール、あるいは
アセトン、プロピレングリコール、１、３−ブチレング
リコール、またはこれらの混合液で抽出した後、常法に
従って、ポリフェノール類を精製濃縮する事で得られ
る。すなわち、これらの植物の樹皮等を乾燥して、細か
く砕いて抽出溶媒で抽出する。抽出溶媒は、通常、原料
の５〜１０倍容量を用いて、２〜３回繰り返し抽出す
る。 また、この抽出は加熱抽出によって行い、原料は
粉砕する事が好ましい。冷却後、濾過して濾液を得る。
濾過の際に得られた残渣は、再び、上記の操作に従って
２回抽出を行い、その濾液を混合し、減圧濃縮等の方法
で濃縮する。抽出には水、エタノール、プロパノール、
ブタノール、あるいはアセトン、プロピレングリコー
ル、１、３−ブチレングリコール、またはこれらの溶媒
を任意の割合で配合した混合液が用いられる。尚、減圧
濃縮は濾液中に含まれる有機溶剤が完全に除去されるま
で行い、水懸濁液または乾固した状態の粗抽出物を製造
する。[0007] The polyphenols and the extract containing the glycoside thereof used in the present invention can be obtained, for example, by
It is obtained by extracting with water, ethanol, propanol, butanol, or acetone, propylene glycol, 1,3-butylene glycol, or a mixture thereof, and then purifying and concentrating the polyphenols according to a conventional method. That is, the bark and the like of these plants are dried, finely ground, and extracted with an extraction solvent. The extraction solvent is usually extracted twice or three times using 5 to 10 times the volume of the raw material. This extraction is preferably performed by heat extraction, and the raw material is preferably ground. After cooling, the mixture is filtered to obtain a filtrate.
The residue obtained at the time of filtration is again extracted twice according to the above operation, and the filtrates are mixed and concentrated by a method such as vacuum concentration. Water, ethanol, propanol,
Butanol, acetone, propylene glycol, 1,3-butylene glycol, or a mixed solution in which these solvents are mixed at an arbitrary ratio are used. The concentration under reduced pressure is performed until the organic solvent contained in the filtrate is completely removed, to produce a water-suspended or dried crude extract.
【０００８】この粗抽出物よりポリフェノール類とその
配糖体を精製濃縮する方法としては、ブタノールあるい
は酢酸エチル等の有機溶媒を用いた分画や、疎水性・親
水性による分画、イオン交換樹脂による分画および分子
量の違い等による分画など、いずれの方法をもちいても
よい。またこれらの濃縮の前に、上記水懸濁液を、例え
ばクロロホルム、ベンゼン、ヘキサン等の非極性溶媒と
混合し、それらに溶解する非極性成分を予め除いておく
事も有用である。この操作によって有効成分をより効率
的に濃縮する事が可能であり、かつ皮膚にアレルギーを
引き起こすような成分を効率的に排除する事も可能とな
る。尚、これらの成分は、そのままの形で化粧料に配合
する事に何ら問題はないが、必要に応じて脱色や脱臭等
の目的のために活性炭等の処理をおこなってもよい。カ
バノキ科、ハンノキ科属植物のポリフェノール類および
その配糖体は、例えばカテキンおよびその配糖体であっ
て、本発明で用いる抽出物では、抽出物全体に対してポ
リフェノール総量として３０重量％以上であることが好
ましい。Methods for purifying and condensing polyphenols and their glycosides from the crude extract include fractionation using an organic solvent such as butanol or ethyl acetate, fractionation based on hydrophobicity / hydrophilicity, and ion exchange resin. Either method may be used, such as fractionation based on molecular weight and fractionation based on differences in molecular weight. It is also useful to mix the aqueous suspension with a non-polar solvent such as chloroform, benzene, hexane or the like before the concentration to remove non-polar components dissolved therein. By this operation, it is possible to concentrate the active ingredient more efficiently, and it is also possible to efficiently remove the ingredient that causes allergy to the skin. It should be noted that there is no problem if these components are added to the cosmetics as they are, but if necessary, treatment with activated carbon or the like may be performed for the purpose of decolorization or deodorization. The polyphenols and their glycosides of birch and alder plants are, for example, catechins and their glycosides. In the extract used in the present invention, the total amount of polyphenols in the extract is 30% by weight or more based on the whole extract. Preferably, there is.
【０００９】本発明で用いる該抽出物のポリフェノール
の含量、及びそれ以外の特性を下記に示す。 （１）ポリフェノール含量：抽出物を精製水に溶解後、
等量のブタノールを加えて激しく混和した後にブタノー
ル可溶成分を集め、この操作をさらに２回繰り返し、全
てのブタノール可溶分を合し、減圧下濃縮乾固したもの
をポリフェノール量と規定した時、その含量は３０重量
％以上である。 （２）確認試験：抽出物を１ｍｇ／ｍｌ水溶液とした後
に塩化第二鉄試液を１〜２滴加えるとき、液が緑かっ色
から暗青色を呈する。 （３）赤外吸収スペクトル（ＫＢｒ）：（νＭａｘ）３
４００、２９３０、１６００、１０５０ｃｍ-1 （４）紫外吸収スペクトル（Ｈ2Ｏ）：（λＭａｘ）２
８０ｎｍ （５）薄層クロマトグラフィー：ＵＶ光下蛍光発色型親
水性シリカゲルプレート（製品名：ＭＥＲＣＫ ＨＰＴ
ＬＣ Ｓｉｌｉｃａ ｇｅｌ ６０Ｆ２５４）でクロロ
ホルム：メタノール＝２：１の展開溶媒で展開した際、
Ｒｆ値０．３８、０．５９を示す。The polyphenol content of the extract used in the present invention and other properties are shown below. (1) Polyphenol content: After dissolving the extract in purified water,
After adding an equal amount of butanol and mixing vigorously, the butanol-soluble component was collected, and this operation was repeated twice more. All butanol-soluble components were combined, and concentrated to dryness under reduced pressure to determine the amount of polyphenol. , Its content is at least 30% by weight. (2) Confirmation test: When 1 to 2 drops of ferric chloride reagent solution is added after the extract is made into 1 mg / ml aqueous solution, the solution takes on a greenish brown to dark blue color. (3) Infrared absorption spectrum (KBr): (νMax) 3
400, 2930, 1600, 1050 cm -1 (4) Ultraviolet absorption spectrum (H 2 O): (λMax) 2
80 nm (5) Thin layer chromatography: Fluorescent colorable hydrophilic silica gel plate under UV light (Product name: MERCK HPT)
When developed with a developing solvent of chloroform: methanol = 2: 1 using LC Silica gel 60F254),
The Rf values are 0.38 and 0.59.
【００１０】本発明の皮膚化粧料における該抽出物含有
量は０．０００１〜１５重量％、特に好ましくは ０．
００１〜５重量％である。配合量が乾燥重量で０．００
０１重量％に満たないと充分な効果は得られず、１５重
量％を超えると着色などの点で配合が難しい。[0010] The content of the extract in the skin cosmetic of the present invention is 0.0001 to 15% by weight, particularly preferably 0.1 to 0.1% by weight.
001 to 5% by weight. Compounding amount is 0.00 by dry weight
If the amount is less than 01% by weight, a sufficient effect cannot be obtained.
【００１１】本発明の皮膚外用剤は、例えば、水溶液
系、可溶化系、乳化系、粉末系、油液系、ゲル系、軟膏
系等の種々の製剤に常法により調製し、クリーム、乳
液、化粧水、美容液、パック、洗顔剤、パウダーなどと
して提供できる。本発明の皮膚外用剤は、上記の成分に
加え、必要に応じ、本発明の効果を損なわない範囲で、
一般に用いられる成分、例えば、油脂成分、界面活性
剤、多価アルコール、また紫外線吸収剤や紫外線散乱
剤、さらに増粘剤、防腐剤、酸化防止剤、香料、色剤、
ビタミンや感光素、プラセンタエキス等の細胞賦活剤、
リノール酸、γ−リノレイン酸、スフィンゴ脂質、セラ
ミド、糖セラミド、ヒアルロン酸等を配合することがで
きる。The external preparation for skin of the present invention can be prepared in various preparations such as aqueous solution, solubilizing system, emulsifying system, powder system, oil-liquid system, gel system, ointment system and the like by usual methods, , Lotions, serums, packs, facial cleansers, powders and the like. The external preparation for skin of the present invention, in addition to the above components, if necessary, within a range that does not impair the effects of the present invention,
Commonly used components, for example, fats and oils components, surfactants, polyhydric alcohols, ultraviolet absorbers and ultraviolet scattering agents, further thickeners, preservatives, antioxidants, fragrances, coloring agents,
Cell activators such as vitamins, photosensitizers and placenta extract,
Linoleic acid, γ-linoleic acid, sphingolipid, ceramide, sugar ceramide, hyaluronic acid and the like can be blended.
【００１２】[0012]
【実施例】次に、実施例を用いて、本発明について更に
詳細に説明する。いうまでもなく本発明はこれら実施例
に限られるものではなく、また特にことわらない限り
［％］は［重量％］を示す。 ＜参考例１：従来のシラカバ抽出物＞シラカバの樹皮１
００ｇを１ｌの水で２時間加熱抽出した。この抽出を２
回繰り返した。抽出液を濾過して合し、減圧濃縮したの
ち乾固し、水抽出物３．７ｇを得た。（ポリフェノール
量 １９．０％）Next, the present invention will be described in more detail with reference to examples. Needless to say, the present invention is not limited to these Examples, and unless otherwise specified, [%] indicates [% by weight]. <Reference Example 1: Conventional birch extract> Birch bark 1
00g was extracted by heating with 1 l of water for 2 hours. This extraction is
Repeated times. The extracts were combined by filtration, concentrated under reduced pressure and then dried to obtain 3.7 g of a water extract. (Polyphenol content 19.0%)
【００１３】＜参考例２＞シラカバの樹皮１００ｇを１
ｌの水：アセトン（１：１）で２時間加熱抽出した。こ
の抽出を２回繰り返した。抽出液を濾過して合し、減圧
濃縮し溶媒を除去した後、同抽出物に５００ｍｌの水お
よびクロロホルムを加え、激しく振り混ぜ、水可溶成分
を分離した。この水可溶成分の水溶液に等容量の酢酸エ
チルを加え、激しく振り混ぜ、酢酸エチル可溶成分を分
離した。この操作を２回繰り返し、それぞれを濾過して
合し、減圧濃縮したのち乾固して当該抽出物０．１９ｇ
を得た。（ポリフェノール量 ９７％）Reference Example 2 100 g of birch bark was added to 1
The mixture was heated and extracted with 1 l of water: acetone (1: 1) for 2 hours. This extraction was repeated twice. The extracts were combined by filtration, concentrated under reduced pressure to remove the solvent, 500 ml of water and chloroform were added to the extract, and the mixture was shaken vigorously to separate water-soluble components. An equal volume of ethyl acetate was added to the aqueous solution of the water-soluble component, and the mixture was vigorously shaken to separate the ethyl acetate-soluble component. This operation was repeated twice, each was filtered, combined, concentrated under reduced pressure, and then dried to obtain 0.19 g of the extract.
I got (Polyphenol content 97%)
【００１４】＜参考例３＞参考例１で得られた水抽出物
１ｇを１００ｍｌの水に溶解させ、それに等容量のヘキ
サンを加えて激しく振り混ぜ、これを２回繰り返してヘ
キサン可溶分を除去した。ついで水可溶成分、すなわち
残分を合した後に減圧濃縮し、シリカゲルカラムクロマ
トグラフィー（１００ｇ：φ３５×２４０ｍｍ）に付
し、クロロホルム：メタノール：水＝７：３：１の下層
〜クロロホルム：メタノール＝２：１の直線グラジエン
トを用いて溶出し、２５４ｎｍ ＵＶ光下蛍光発色型親
水性シリカゲルプレート（製品名：ＨＰＴＬＣ Ｓｉｌ
ｉｃａ ｇｅｌ ６０Ｆ２５４）でクロロホルム：メタ
ノール：水＝６５：３５：１０の下層を展開溶媒として
展開した際、Ｒｆ値が０．２１〜０．６４の分画を得
た。その後、その分画を合し、減圧濃縮したのち乾固
し、当該抽出物０．３２ｇを得た。（ポリフェノール量
７４％）。<Reference Example 3> 1 g of the water extract obtained in Reference Example 1 was dissolved in 100 ml of water, an equal volume of hexane was added thereto, and the mixture was shaken vigorously. Removed. Then, the water-soluble components, that is, the residue, were combined, concentrated under reduced pressure, and subjected to silica gel column chromatography (100 g: φ35 × 240 mm) to form chloroform: methanol: water = 7: 3: 1 lower layer to chloroform: methanol = Elute using a 2: 1 linear gradient and elute using a fluorescent silica gel plate (product name: HPTLC Sil) under 254 nm UV light.
When the lower layer of chloroform: methanol: water = 65: 35: 10 was developed using ica gel 60F254) as a developing solvent, fractions having Rf values of 0.21 to 0.64 were obtained. Then, the fractions were combined, concentrated under reduced pressure, and dried to obtain 0.32 g of the extract. (Polyphenol content 74%).
【００１５】次に、参考例１〜３を用いて老化防止効果
を評価した。評価方法を示す。 １． ターンオーバー促進効果 ２年齢の雌性ハートレー系モルモットの背部を刈毛後、
ダンシルクロライド（０．１５ｇ）を白色ワセリン
（３．０ｇ）に練り込んだものをモルモット背部に鳥居
のパッチ板を用いて０．１ｍｌ閉塞貼付し２４時間放置
した。２４時間後パッチ板を除去し、さらに２４時間放
置し、その翌日より参考例１と参考例３の１％（Ｗ／
Ｖ）５０％エタノール溶液、およびそれらを溶解した５
０％エタノール（溶媒対照）をダンシルクロライド処置
部位に０．０５ｍｌずつ１６日間、１日１回塗布した。
ターンオーバー速度は、試験サンプルを塗布した部位の
角質層に付着したダンシルクロライドの輝度を輝度計
（ミノルタＬＳ−１００）により測定し、試験期間中の
経日的な変化として表わした。その結果（ｎ＝５の平
均）を図１に示した。Next, the anti-aging effect was evaluated using Reference Examples 1 to 3. The evaluation method will be described. 1. Turnover promotion effect After shaving the back of a 2 year old female Hartley guinea pig,
A mixture of dansyl chloride (0.15 g) and white petrolatum (3.0 g) was adhered to the back of a guinea pig using a torii patch plate, and 0.1 ml of the mixture was stuck and left for 24 hours. Twenty-four hours later, the patch plate was removed, and the plate was allowed to stand for another 24 hours. From the next day, 1% (W / W) of Reference Examples 1 and 3 was used.
V) 50% ethanol solution and their dissolved 5
0% ethanol (solvent control) was applied to the dansyl chloride treated site once a day for 16 days in a volume of 0.05 ml.
The turnover speed was measured by measuring the luminance of dansyl chloride attached to the stratum corneum at the site where the test sample was applied using a luminance meter (Minolta LS-100) and expressed as a daily change during the test period. The result (average of n = 5) is shown in FIG.
【００１６】[0016]
【図１】 FIG.
【００１７】図１から、従来のシラカバ抽出物（参考例
１）および本発明のシラカバ抽出物（参考例３）塗布に
よって、溶媒対照と比較して角質層ターンオーバーが顕
著に促進された。特に参考例３を塗布した部位の輝度の
減少は顕著で、参考例１を上廻る角質層ターンオーバー
の促進が認められた。FIG. 1 shows that the application of the conventional birch extract (Reference Example 1) and the birch extract of the present invention (Reference Example 3) significantly promoted the stratum corneum turnover as compared with the solvent control. In particular, the decrease in the luminance of the portion to which Reference Example 3 was applied was remarkable, and the promotion of the horny layer turnover exceeding Reference Example 1 was observed.
【００１８】２． 抗酸化効果 ＳＯＤ様活性を以下の方法で測定した。すなわち、試験
管に１本当り、０．０５Ｍ炭酸緩衝液 （ｐＨ１０．
２）２．４ｍｌ、３ｍＭキサンチン溶液、３ｍＭＥＤＴ
Ａ・２ナトリウム水溶液、 ０．１５（Ｗ／Ｖ）％ Ｂ
ＳＡ水溶液、０．７５ｍＭニトロブルーテトラゾリウム
水溶液、各０．１ｍｌを混合し、これに検体 ０．１ｍ
ｌを加え、２５℃で１０分間放置後にキサンチンオキシ
ターゼ水溶液０．１ｍｌを加えて手早く撹拌し、２５℃
でインキュベートした。 ２０分後に６ｍＭ塩化第二銅
水溶液 ０．１ｍｌを加えて酵素反応を停止させ、５６
０ｎｍでの吸光度（Ａ）を測定した。対照には、検体の
代わりに検体希釈溶媒を加えた吸光度（Ｂ）を、また、
各試料のブランクには、検体を加えた後、６ｍＭ塩化第
二銅水溶液 ０．１ｍｌを加え、同様にキサンチンオキ
シダーゼ水溶液０．１ｍｌを添加した時の吸光度（Ｃ）
を測定した。 阻害率（％）は下記の計算式より算出
し、図２に示した。2. Antioxidant effect SOD-like activity was measured by the following method. That is, 0.05 M carbonate buffer (pH 10.
2) 2.4 ml, 3 mM xanthine solution, 3 mM EDT
A ・ 2 sodium aqueous solution, 0.15 (W / V)% B
0.1 ml each of an aqueous SA solution and a 0.75 mM nitro blue tetrazolium aqueous solution was mixed with each
l, and the mixture was allowed to stand at 25 ° C for 10 minutes, and then 0.1 ml of an aqueous solution of xanthine oxidase was added thereto.
Incubated. After 20 minutes, 0.1 ml of a 6 mM aqueous cupric chloride solution was added to stop the enzyme reaction.
The absorbance (A) at 0 nm was measured. For the control, absorbance (B) obtained by adding a sample diluting solvent instead of the sample,
To the blank of each sample, after adding the specimen, 0.1 ml of a 6 mM aqueous cupric chloride solution was added, and similarly, the absorbance when adding 0.1 ml of an aqueous solution of xanthine oxidase (C)
Was measured. The inhibition rate (%) was calculated by the following formula and shown in FIG.
【００１９】[0019]
【式１】 (Equation 1)
【００２０】[0020]
【図２】 FIG. 2
【００２１】図２から明らかなように、本発明の抽出物
（参考例２）には、従来のシラカバ抽出物（参考例１）
以上の抗酸化力が認められた。次に、実施例１を用いて
老化防止効果（美肌、シワ改善効果）を評価した。処方
と評価方法を示す。 実施例１：クリーム 成分 配合量（重量％） 成分（Ａ） ポリグリセリン脂肪酸エステル ４．０ セタノール ２．０ ステアリン酸 １．０ ミリスチン酸イソプロピル ７．０ スクワラン ９．０ 自己乳化型モノステアリン酸グリセリル ３．０ 成分（Ｂ） 参考例３の抽出物 ０．５ プロピレングリコール ５．０ 香料 ０．１ 精製水 残量 成分（Ｃ） 水酸化カリウム水溶液 ３．０ 合計 １００．０ （製法）成分（Ａ）を加熱溶解する。別に香料を除く成
分（Ｂ）を加熱溶解して、これに前記成分（Ａ）を撹拌
しながら加えて、充分混合する。さらに成分（Ｃ）を加
え、撹拌しながら冷却を行い、香料を加え、さらに混合
してクリームを得た。As is apparent from FIG. 2, the extract of the present invention (Reference Example 2) is different from the conventional birch extract (Reference Example 1).
The above antioxidant power was recognized. Next, the aging prevention effect (beautiful skin, wrinkle improvement effect) was evaluated using Example 1. The prescription and evaluation method are shown. Example 1: Cream Ingredients Compounding amount (% by weight) Ingredient (A) Polyglycerin fatty acid ester 4.0 Cetanol 2.0 Stearic acid 1.0 Isopropyl myristate 7.0 Squalane 9.0 Self-emulsifying glyceryl monostearate 3 0.0 Component (B) Extract of Reference Example 3 0.5 Propylene glycol 5.0 Fragrance 0.1 Purified water Remaining component (C) Potassium hydroxide aqueous solution 3.0 Total 100.0 (Production method) Component (A) Is heated and dissolved. Separately, the component (B) excluding the fragrance is dissolved by heating, and the component (A) is added thereto with stirring and mixed well. Further, the component (C) was added, the mixture was cooled with stirring, a flavor was added, and the mixture was further mixed to obtain a cream.
【００２２】３． 老化防止効果 荒れ肌、小シワ、くすみ、シミなどの肌の老徴を訴える
女子被験者（３４〜５５才）３０名に実施例１（クリー
ムＡ）および実施例１において参考例３無配合の対照
（クリームＢ）を１日２回（朝、夜）ハーフフェイスで
２ヶ月間使用させ、２ヶ月後の効果を問診によって検討
した。評価は、小じわの程度、シミの程度、肌のつや、
くすみの程度、肌の柔軟性、弾力性、肌のしっとり感に
関し、被験者自身に各々下記に示す３段階で評価させ
た。結果を表１に示す。3. Anti-aging effect Example 1 (cream A) and 30-year-old female subjects (34-55 years old) who complain of aging signs of the skin such as rough skin, wrinkles, dullness, spots, etc. Cream B) was used twice a day (morning and evening) with a half face for 2 months, and the effect after 2 months was examined by interview. Evaluation is the degree of fine wrinkles, the degree of spots, the gloss of the skin,
The degree of dullness, skin softness, elasticity, and moist feeling of the skin were evaluated by the subject himself according to the following three grades. Table 1 shows the results.
【００２３】[0023]
【表１】 [Table 1]
【００２４】表１のように、実施例は比較例に比べて、
小じわ、シミ、つやの低減、くすみ、柔軟性、弾力性の
改善、また乾燥などの改善度に優れ、老化現象にきわめ
て有用であると認められた。As shown in Table 1, the embodiment is different from the comparative example in that
It was found to be extremely useful for aging due to its excellent degree of improvement such as reduction of fine lines, stains and gloss, dullness, flexibility, elasticity, and drying.
【００２５】 実施例２：軟膏 成分 配合量（重量％） 参考例２で得た抽出物 ５．０ プロピレングリコール １５．０ マクロゴール軟膏 ８０．０ 合計 １００．０ （製法）参考例２で得た抽出物をプロピレングリコール
に均一に分散させた後、マクロゴール軟膏を加えて混合
する。Example 2: Ointment Ingredients Compounding amount (% by weight) Extract obtained in Reference Example 2 5.0 Propylene glycol 15.0 Macrogol ointment 80.0 Total 100.0 (Preparation method) Obtained in Reference Example 2 After the extract is uniformly dispersed in propylene glycol, macrogol ointment is added and mixed.
【００２６】 実施例３：化粧水 成分 配合量（重量％） 参考例３で得た抽出物 １．０ グリセリン ６．０ エタノール ９．０ ポリオキシエチレン硬化ヒマシ油 ０．８ クエン酸 ０．０５ クエン酸ナトリウム ０．０８ 香料 ０．１ 精製水 残部 合計 １００．０ （製法）精製水にグリセリン、クエン酸、クエン酸ナト
リウム、参考例３で得た抽出物を溶解する。個別にエタ
ノールにポリオキシエチレン硬化ヒマシ油（６０Ｅ．
Ｏ．）、香料を溶解し、前記の水溶液に加えて化粧水を
得た。Example 3: Lotion Component Content (% by weight) Extract obtained in Reference Example 3 1.0 Glycerin 6.0 Ethanol 9.0 Polyoxyethylene hydrogenated castor oil 0.8 Citric acid 0.05 Citric acid Sodium acid 0.08 Perfume 0.1 Purified water Remainder Total 100.0 (Preparation method) Glycerin, citric acid, sodium citrate, and the extract obtained in Reference Example 3 are dissolved in purified water. Polyoxyethylene hydrogenated castor oil (60E.
O. ) And perfume were dissolved and added to the aqueous solution to obtain a lotion.
【００２７】 実施例５：乳液 成分 配合量（重量％） 成分（Ａ） 流動パラフィン ５．０ ワセリン ２．０ ミツロウ １．０ セスキオレイン酸ソルビタン ２．０ 成分（Ｂ） 参考例３で得た抽出物 ０．１ イプシロンアミノカプロン酸 ０．２ ポリオキシエチレンオレイルエーテル（２０Ｅ．Ｏ．） ２．５ プロピレングリコール ５．０ カルボキシビニルポリマー ０．５ トリエタノールアミン ０．５ 香料 ０．２ 精製水 残部 合計 １００．０ （製法）成分（Ａ）を加熱溶解し、別に加温溶解した香
料を除く成分（Ｂ）に撹拌しながら冷却を行い、香料を
加え、さらに冷却して乳液を得た。Example 5: Emulsion Ingredients Compounding amount (% by weight) Ingredient (A) Liquid paraffin 5.0 Vaseline 2.0 Beeswax 1.0 Sorbitan sesquioleate 2.0 Ingredient (B) Extraction obtained in Reference Example 3 Compound 0.1 Epsilon aminocaproic acid 0.2 Polyoxyethylene oleyl ether (20EO) 2.5 Propylene glycol 5.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 Perfume 0.2 Purified water Remainder Total 100 0.0 (Preparation method) The component (A) was heated and dissolved, and the component (B) excluding the flavor which was separately heated and dissolved was cooled with stirring, the flavor was added, and the emulsion was further cooled to obtain an emulsion.
【００２８】 実施例６：パック 成分 配合量（重量％） 参考例２で得た抽出物 ５．０ 酢酸ビニル・スチレン共重合体 １０．０ ポリビニルアルコール １０．０ ソルビット ５．０ エタノール ５．０ 香料 ２．０ 精製水 残部 合計 １００．０ （製法）参考例２で得た抽出物、香料、及びエタノール
を均一に溶解する。これを酢酸ビニル・スチレン共重合
体、ポリビニルアルコール、ソルビットを混合したもの
に加える。Example 6: Pack Ingredients Compounding amount (% by weight) Extract obtained in Reference Example 2 5.0 Vinyl acetate / styrene copolymer 10.0 Polyvinyl alcohol 10.0 Sorbit 5.0 Ethanol 5.0 Perfume 2.0 Purified water Remainder Total 100.0 (Preparation method) The extract, flavor, and ethanol obtained in Reference Example 2 are uniformly dissolved. This is added to a mixture of vinyl acetate / styrene copolymer, polyvinyl alcohol and sorbit.
【００２９】[0029]
【発明の効果】本発明の皮膚外用剤は、角質層のターン
オーバー促進（新陳代謝促進）作用、抗酸化作用、さら
には、小じわ、シミ、くすみの低減、肌につや、柔軟
性、弾力性、しっとり感を付与する効果を有し、皮膚の
老化防止やその改善に効果を発揮し、老化防止用化粧料
として有用であると認められた。EFFECT OF THE INVENTION The external preparation for skin of the present invention has an effect of promoting the turnover of the stratum corneum (promoting metabolism), an antioxidant effect, a reduction in fine lines, stains and dullness, a luster, softness, elasticity, It has the effect of imparting a moist feeling, is effective in preventing and improving skin aging, and is recognized as being useful as an antiaging cosmetic.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 小林 聡美 大阪府高槻市朝日町３番１号 サンスタ− 株式会社内 (72)発明者 石角 篤 大阪府高槻市朝日町３番１号 サンスタ− 株式会社内 (72)発明者 小林 泰信 大阪府高槻市朝日町３番１号 サンスタ− 株式会社内 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Satomi Kobayashi 3-1 Asahi-cho, Takatsuki-shi, Osaka Sunstar Co., Ltd. (72) Inventor Atsushi Ishizumi 3-1 Asahi-cho, Takatsuki-shi, Osaka Sunstar Co., Ltd. In-company (72) Inventor Yasunobu Kobayashi 3-1 Asahi-cho, Takatsuki-shi, Osaka Sunstar Co., Ltd.
Claims (2)
抽出物全体に対してポリフェノールとして３０重量％以
上含有する、カバノキ科カバノキ属及びハンノキ属植物
の抽出物を配合することを特徴とする皮膚外用剤。1. A polyphenol and a glycoside thereof,
An external preparation for skin, comprising an extract of a plant of the birch family, birch and alder, containing 30% by weight or more as a polyphenol based on the whole extract.
物の抽出物が水、エタノール、プロパノール、アセト
ン、プロピレングリコール、１、３−ブチレングリコー
ルからなる群から選ばれる１種以上を用いて抽出された
ものであることを特徴とする請求項１に記載された皮膚
外用剤。2. An extract of a plant of the birch genus birch and alder of the birch family extracted using at least one selected from the group consisting of water, ethanol, propanol, acetone, propylene glycol and 1,3-butylene glycol. The external preparation for skin according to claim 1, wherein
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9356157A JPH11171722A (en) | 1997-12-09 | 1997-12-09 | Preparation for external use for skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9356157A JPH11171722A (en) | 1997-12-09 | 1997-12-09 | Preparation for external use for skin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH11171722A true JPH11171722A (en) | 1999-06-29 |
Family
ID=18447622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9356157A Pending JPH11171722A (en) | 1997-12-09 | 1997-12-09 | Preparation for external use for skin |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH11171722A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001072568A (en) * | 1999-09-07 | 2001-03-21 | Sunstar Inc | Skin cosmetic |
| JP2005350407A (en) * | 2004-06-11 | 2005-12-22 | P & P F:Kk | Skin care preparation for external use for improving skin drabness |
| WO2006014100A1 (en) * | 2004-08-05 | 2006-02-09 | Martinez Castillejos Guillermo | Pharmaceutical formulation of herbal medicines based on phytolacca americana, alnus acuminata extracts and coal tar for treating and curing psoriasis |
| WO2006068254A1 (en) * | 2004-12-24 | 2006-06-29 | Suntory Limited | Skin-whitening agent |
| JP2008266156A (en) * | 2007-04-17 | 2008-11-06 | Kao Corp | Heparin-binding epidermal growth factor-like growth factor gene expression promoter containing plant extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001072568A (en) * | 1999-09-07 | 2001-03-21 | Sunstar Inc | Skin cosmetic |
| JP2005350407A (en) * | 2004-06-11 | 2005-12-22 | P & P F:Kk | Skin care preparation for external use for improving skin drabness |
| WO2006014100A1 (en) * | 2004-08-05 | 2006-02-09 | Martinez Castillejos Guillermo | Pharmaceutical formulation of herbal medicines based on phytolacca americana, alnus acuminata extracts and coal tar for treating and curing psoriasis |
| WO2006068254A1 (en) * | 2004-12-24 | 2006-06-29 | Suntory Limited | Skin-whitening agent |
| JPWO2006068254A1 (en) * | 2004-12-24 | 2008-06-12 | サントリー株式会社 | Whitening agent |
| JP2012116847A (en) * | 2004-12-24 | 2012-06-21 | Suntory Holdings Ltd | Skin-whitening agent |
| JP5095221B2 (en) * | 2004-12-24 | 2012-12-12 | サントリーホールディングス株式会社 | Whitening agent |
| JP2008266156A (en) * | 2007-04-17 | 2008-11-06 | Kao Corp | Heparin-binding epidermal growth factor-like growth factor gene expression promoter containing plant extract |
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