JPH1036282A - Agent for suppressing breakage of mucopolysaccharide - Google Patents
Agent for suppressing breakage of mucopolysaccharideInfo
- Publication number
- JPH1036282A JPH1036282A JP8207980A JP20798096A JPH1036282A JP H1036282 A JPH1036282 A JP H1036282A JP 8207980 A JP8207980 A JP 8207980A JP 20798096 A JP20798096 A JP 20798096A JP H1036282 A JPH1036282 A JP H1036282A
- Authority
- JP
- Japan
- Prior art keywords
- mucopolysaccharide
- plant
- cleavage
- water
- essence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Confectionery (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Seasonings (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、ムコ多糖類切断抑
制剤及びそれを含有する医薬、化粧料、食品などの組成
物に関する。TECHNICAL FIELD The present invention relates to a mucopolysaccharide cleavage inhibitor and a composition containing the same, such as a medicament, a cosmetic or a food.
【0002】[0002]
【従来の技術】ヒアルロン酸ナトリウムに代表されるム
コ多糖類は、生体に於いて皮膚或いは関節などに存在
し、保水作用、潤滑作用或いは弾性維持作用等の作用に
より生体を維持して行くのに重要な役割を担っている。
例えば、化学物質によってムコ多糖類が切断されたり、
光などによって架橋されたりすると皮膚は弾性を失いし
わなどを形成しやすくなるし、関節などに於いて、激し
い運動などによりムコ多糖類が切断されたりすると本来
の潤滑能力を失い、関節痛等の疾病を引き起こす。この
様なムコ多糖類の切断による機能不全に対して現在為し
うることは、生体外よりムコ多糖類を補充することぐら
いしか無い。即ち、ムコ多糖類の切断などによる機能不
全に対して、切断を抑制し機能不全に陥らないようにす
る手段は求められていたにもかかわらず得られていなか
った。2. Description of the Related Art Mucopolysaccharides typified by sodium hyaluronate are present in the skin or joints of a living body, and are used to maintain the living body by actions such as water retention, lubrication or elasticity. Plays an important role.
For example, mucopolysaccharides are cleaved by chemicals,
When crosslinked by light or the like, the skin loses elasticity and easily forms wrinkles.When mucopolysaccharides are cut by intense exercise in joints, etc., the original lubricating ability is lost, and joint pain etc. Causes disease. The only thing that can be done now for such dysfunction due to mucopolysaccharide cleavage is to replenish mucopolysaccharide from outside the body. That is, in spite of the demand for a means for preventing the dysfunction caused by the cleavage of the mucopolysaccharide or the like so that the dysfunction does not occur, no means has been obtained.
【0003】[0003]
【発明が解決しようとする課題】本発明はこの様な状況
下行われたものであり、ムコ多糖類の切断を抑制する手
段を提供することを課題とする。DISCLOSURE OF THE INVENTION The present invention has been made under such circumstances, and it is an object of the present invention to provide means for suppressing the cleavage of mucopolysaccharide.
【0004】[0004]
【課題を解決するための手段】この様な状況に鑑みて、
本発明者等はムコ多糖類の切断を抑制する作用を有する
物質を求めて鋭意研究を重ねた結果、シソ科植物のエッ
センスにその様な作用を見いだした。更に検討を重ねた
結果、これらシソ科植物のエッセンスを化粧料、食品、
医薬品に含有させ、投与することによりムコ多糖類の切
断を抑制しうることを見いだし発明を完成させた。以
下、本発明について発明の実施の形態を中心に詳細に説
明する。In view of such a situation,
The present inventors have conducted intensive studies for a substance having an action of inhibiting the cleavage of mucopolysaccharide, and as a result, have found such an action in the essence of a Labiatae plant. As a result of further studies, the essence of these Labiatae plants was applied to cosmetics,
The inventor of the present invention has found that it is possible to suppress the cleavage of mucopolysaccharides by containing the compound in a pharmaceutical and administering it, thereby completing the invention. Hereinafter, the present invention will be described in detail focusing on embodiments of the invention.
【0005】[0005]
(1)本発明のムコ多糖類切断抑制剤 本発明のムコ多糖類切断抑制剤はシソ科植物のエッセン
スからなる。ここでエッセンスとは、植物体の一部又は
全部それ自身、植物体の一部又は全部を乾燥、粉砕、細
切などした加工物、植物体の一部又は全部或いはそれら
の加工物に溶媒を加えて処理した抽出物、それらのカラ
ムクロマトグラフィーや液液抽出などによる分画物等の
総称を意味する。本発明で用いることの出来るシソ科植
物としては、例えば、スペアミント、ペッパーミント、
ウォーターミント、日本ハッカ、レモンバーム、キャッ
トニップ、セージ、ローズマリー、マージョラム、タイ
ム、タラゴン、ラベンダー等が好ましく例示できる。こ
れらの内好ましいものは、スペアーミント、ペッパーミ
ント、ウォーターミント、日本ハッカ、セージ、ローズ
マリーである。中でも、スペアーミントとウォーターミ
ントの掛け合わせ品種とセージの中のサルビア・オフィ
シナリス・バール・ラティフォリアとサルビア・オフィ
シナリス・バール・アルバの掛け合わせ品種が特に好ま
しい。又、エッセンスとしては抽出物又はその溶媒溜去
物が好ましい。抽出は植物体又はその加工物に対して1
〜50倍量の溶媒を加え、室温であれば数日、沸点付近
の温度であれば数時間浸漬すれば良い。溶媒としては、
例えばメタノールやエタノール、1,3−ブタンジオー
ル等のアルコール類、酢酸エチルや蟻酸メチル等のエス
テル類、アセトニトリル等のニトリル類、ジエチルエー
テルやテトラヒドロフラン等のエーテル類、クロロホル
ムや塩化メチレン等のハロゲン化炭化水素類、アセトン
やメチルエチルケトン等のケトン類、水等が例示でき
る。これらの溶媒は唯一種を単独で用いても良いし、二
種以上を混合して用いても良い。これらの内最も好まし
いものは、30〜70%エタノール水溶液である。かか
る、抽出物をダイアイオンHP−20にチャージして水
洗した後、20%エタノール水溶液で溶出させ溶媒溜去
したものが更に好ましい。かくして得られたエッセンス
は試験例に示すように優れたムコ多糖類の切断を抑制す
る作用を有する。(1) Mucopolysaccharide cleavage inhibitor of the present invention The mucopolysaccharide cleavage inhibitor of the present invention comprises the essence of a Labiatae plant. Here, the essence refers to a part or all of the plant itself, a processed product obtained by drying, pulverizing, shredding, or the like, a part or the whole of the plant, a solvent for a part or all of the plant, or a processed product thereof. In addition, it refers to the collective term for extracts that have been processed, and fractions obtained by column chromatography, liquid-liquid extraction, and the like. As Labiatae plants that can be used in the present invention, for example, spearmint, peppermint,
Water mint, Japanese peppermint, lemon balm, catnip, sage, rosemary, marjoram, thyme, tarragon, lavender and the like can be preferably exemplified. Preferred among these are spare mint, peppermint, water mint, Japanese peppermint, sage, and rosemary. Among them, a cross cultivar of spare mint and water mint and a cross cultivar of Salvia officinalis bar latifolia and salvia officinalis bar alba in sage are particularly preferred. As the essence, an extract or a solvent distillate thereof is preferable. Extraction is performed on 1 plant or its processed product.
A solvent of about 50 times the amount may be added, and immersion may be performed at room temperature for several days or at a temperature near the boiling point for several hours. As the solvent,
For example, alcohols such as methanol, ethanol and 1,3-butanediol, esters such as ethyl acetate and methyl formate, nitriles such as acetonitrile, ethers such as diethyl ether and tetrahydrofuran, and halogenated carbons such as chloroform and methylene chloride. Examples include hydrogens, ketones such as acetone and methyl ethyl ketone, and water. These solvents may be used alone or as a mixture of two or more. The most preferred of these is a 30-70% aqueous ethanol solution. More preferably, the extract is charged into Diaion HP-20, washed with water, and then eluted with a 20% aqueous ethanol solution to evaporate the solvent. The essence thus obtained has an excellent action of suppressing the cleavage of mucopolysaccharide as shown in the test examples.
【0006】[製造例1]スペアーミントの生葉1Kg
に30lの50%エタノール水溶液を加え、2時間リフ
ラックスさせた。濾過した後濃縮し蒸留水で溶解分散さ
せ、蒸留水で充填したダイアイオンHP−20にチャー
ジし1lの水で水洗し、20%エタノール水溶液2lを
流して溶出させ、これを減圧溜去し31gのムコ多糖類
切断抑制剤1を得た。[Production Example 1] 1 kg of fresh leaves of spare mint
Then, 30 l of a 50% aqueous ethanol solution was added thereto, and the mixture was refluxed for 2 hours. After filtration, the mixture was concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water, and eluted by flowing 2 l of a 20% aqueous ethanol solution. The mucopolysaccharide cleavage inhibitor 1 was obtained.
【0007】[製造例2]ペッパーミントの生葉1Kg
に30lの50%エタノール水溶液を加え、2時間リフ
ラックスさせた。濾過した後濃縮し蒸留水で溶解分散さ
せ、蒸留水で充填したダイアイオンHP−20にチャー
ジし1lの水で水洗し、20%エタノール水溶液2lを
流して溶出させ、これを減圧溜去し34gのムコ多糖類
切断抑制剤2を得た。[Production Example 2] 1 kg of fresh leaf of peppermint
Then, 30 l of a 50% aqueous ethanol solution was added thereto, and the mixture was refluxed for 2 hours. After filtration, the mixture is concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water, and eluted by flowing 2 l of a 20% aqueous ethanol solution. Of mucopolysaccharide cleavage inhibitor 2 was obtained.
【0008】[製造例3]ウォーターミントの生葉1K
gに30lの50%エタノール水溶液を加え、2時間リ
フラックスさせた。濾過した後濃縮し蒸留水で溶解分散
させ、蒸留水で充填したダイアイオンHP−20にチャ
ージし1lの水で水洗し、20%エタノール水溶液2l
を流して溶出させ、これを減圧溜去し29gのムコ多糖
類切断抑制剤3を得た。[Production Example 3] Fresh leaf of water mint 1K
30 g of a 50% aqueous ethanol solution was added to g, and the mixture was refluxed for 2 hours. After filtration, the mixture was concentrated, dissolved and dispersed in distilled water, charged to DIAION HP-20 filled with distilled water, washed with 1 l of water, and washed with 2 l of a 20% aqueous ethanol solution.
And eluted under reduced pressure to give 29 g of mucopolysaccharide cleavage inhibitor 3.
【0009】[製造例4]スペアーミントとウォーター
ミントの掛け合わせ品種の生葉1Kgに30lの50%
エタノール水溶液を加え、2時間リフラックスさせた。
濾過した後濃縮し蒸留水で溶解分散させ、蒸留水で充填
したダイアイオンHP−20にチャージし1lの水で水
洗し、20%エタノール水溶液2lを流して溶出させ、
これを減圧溜去し36gのムコ多糖類切断抑制剤4を得
た。[Production Example 4] 1 liter of fresh leaves of a hybrid variety of spare mint and water mint and 30 l of 50%
An aqueous ethanol solution was added, and the mixture was refluxed for 2 hours.
After filtration, the mixture was concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water, and eluted by flowing 2 l of a 20% aqueous ethanol solution.
This was distilled under reduced pressure to obtain 36 g of mucopolysaccharide cleavage inhibitor 4.
【0010】[製造例5]日本ハッカの生葉1Kgに3
0lの50%エタノール水溶液を加え、2時間リフラッ
クスさせた。濾過した後濃縮し蒸留水で溶解分散させ、
蒸留水で充填したダイアイオンHP−20にチャージし
1lの水で水洗し、20%エタノール水溶液2lを流し
て溶出させ、これを減圧溜去し23gのムコ多糖類切断
抑制剤5を得た。[Production Example 5] 3 parts per 1 kg of fresh leaves of Japanese peppermint
0 l of a 50% aqueous ethanol solution was added and refluxed for 2 hours. After filtration, concentrated, dissolved and dispersed in distilled water,
DIAION HP-20 filled with distilled water was charged, washed with 1 l of water, eluted by flowing 2 l of a 20% aqueous ethanol solution, and distilled under reduced pressure to obtain 23 g of mucopolysaccharide cleavage inhibitor 5.
【0011】[製造例6]サルビア・オフィシナリス・
バール・ラティフォリアの生葉1Kgに30lの50%
エタノール水溶液を加え、2時間リフラックスさせた。
濾過した後濃縮し蒸留水で溶解分散させ、蒸留水で充填
したダイアイオンHP−20にチャージし1lの水で水
洗し、20%エタノール水溶液2lを流して溶出させ、
これを減圧溜去し18gのムコ多糖類切断抑制剤6を得
た。[Production Example 6] Salvia officinalis
30kg 50% of 1kg of fresh leaves of Bar Latifolia
An aqueous ethanol solution was added, and the mixture was refluxed for 2 hours.
After filtration, the mixture was concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water, and eluted by flowing 2 l of a 20% aqueous ethanol solution.
This was distilled under reduced pressure to obtain 18 g of mucopolysaccharide cleavage inhibitor 6.
【0012】[製造例7]サルビア・オフィシナリス・
バール・アルバの生葉1Kgに30lの50%エタノー
ル水溶液を加え、2時間リフラックスさせた。濾過した
後濃縮し蒸留水で溶解分散させ、蒸留水で充填したダイ
アイオンHP−20にチャージし1lの水で水洗し、2
0%エタノール水溶液2lを流して溶出させ、これを減
圧溜去し15gのムコ多糖類切断抑制剤7を得た。[Production Example 7] Salvia officinalis
30 l of a 50% aqueous ethanol solution was added to 1 kg of fresh leaves of Barr alba, and the mixture was refluxed for 2 hours. After filtration, the mixture is concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water,
Elution was carried out by flowing 2 liters of a 0% aqueous ethanol solution, which was distilled off under reduced pressure to obtain 15 g of mucopolysaccharide cleavage inhibitor 7.
【0013】[製造例8]サルビア・オフィシナリス・
バール・ラティフォリアとサルビア・オフィシナリス・
バール・アルバの掛け合わせ品種の生葉1Kgに30l
の50%エタノール水溶液を加え、2時間リフラックス
させた。濾過した後濃縮し蒸留水で溶解分散させ、蒸留
水で充填したダイアイオンHP−20にチャージし1l
の水で水洗し、20%エタノール水溶液2lを流して溶
出させ、これを減圧溜去し20gのムコ多糖類切断抑制
剤8を得た。[Production Example 8] Salvia officinalis
Bar Latifolia and Salvia Officinalis
30 l for 1 kg of fresh leaves of the crossed varieties of bur alba
Was added thereto, and refluxed for 2 hours. Filter, concentrate, dissolve and disperse in distilled water, charge DIAION HP-20 filled with distilled water and add 1 l
The mixture was washed with water, and eluted by flowing 2 liters of a 20% aqueous ethanol solution, which was distilled under reduced pressure to obtain 20 g of mucopolysaccharide cleavage inhibitor 8.
【0014】[製造例9]レモンバームの生葉1Kgに
30lの50%エタノール水溶液を加え、2時間リフラ
ックスさせた。濾過した後濃縮し蒸留水で溶解分散さ
せ、蒸留水で充填したダイアイオンHP−20にチャー
ジし1lの水で水洗し、20%エタノール水溶液2lを
流して溶出させ、これを減圧溜去し23gのムコ多糖類
切断抑制剤9を得た。Production Example 9 To 1 kg of fresh leaves of lemon balm, 30 l of a 50% aqueous ethanol solution was added and refluxed for 2 hours. After filtration, the mixture was concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water, and eluted by flowing 2 l of a 20% aqueous ethanol solution. The mucopolysaccharide cleavage inhibitor 9 was obtained.
【0015】[製造例10]キャットニップの生葉1K
gに30lの50%エタノール水溶液を加え、2時間リ
フラックスさせた。濾過した後濃縮し蒸留水で溶解分散
させ、蒸留水で充填したダイアイオンHP−20にチャ
ージし1lの水で水洗し、20%エタノール水溶液2l
を流して溶出させ、これを減圧溜去し17gのムコ多糖
類切断抑制剤10を得た。[Production Example 10] Fresh leaves of catnip 1K
30 g of a 50% aqueous ethanol solution was added to g, and the mixture was refluxed for 2 hours. After filtration, the mixture was concentrated, dissolved and dispersed in distilled water, charged to DIAION HP-20 filled with distilled water, washed with 1 l of water, and washed with 2 l of a 20% aqueous ethanol solution.
And eluted under reduced pressure to obtain 17 g of mucopolysaccharide cleavage inhibitor 10.
【0016】[製造例11]マージョラムの生葉1Kg
に30lの50%エタノール水溶液を加え、2時間リフ
ラックスさせた。濾過した後濃縮し蒸留水で溶解分散さ
せ、蒸留水で充填したダイアイオンHP−20にチャー
ジし1lの水で水洗し、20%エタノール水溶液2lを
流して溶出させ、これを減圧溜去し28gのムコ多糖類
切断抑制剤11を得た。[Production Example 11] 1 kg of fresh leaves of marjoram
Then, 30 l of a 50% aqueous ethanol solution was added thereto, and the mixture was refluxed for 2 hours. After filtration, the mixture is concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water, and eluted by flowing 2 l of a 20% aqueous ethanol solution. The mucopolysaccharide cleavage inhibitor 11 was obtained.
【0017】[製造例12]タイムの生葉1Kgに30
lの50%エタノール水溶液を加え、2時間リフラック
スさせた。濾過した後濃縮し蒸留水で溶解分散させ、蒸
留水で充填したダイアイオンHP−20にチャージし1
lの水で水洗し、20%エタノール水溶液2lを流して
溶出させ、これを減圧溜去し11gのムコ多糖類切断抑
制剤12を得た。[Production Example 12] 30 kg per 1 kg of fresh thyme leaves
One liter of a 50% aqueous ethanol solution was added, and the mixture was refluxed for 2 hours. After filtration, the mixture was concentrated, dissolved and dispersed in distilled water, and charged into DIAION HP-20 filled with distilled water to obtain 1
After washing with 1 l of water, 2 l of a 20% ethanol aqueous solution was flowed and eluted, and this was distilled under reduced pressure to obtain 11 g of mucopolysaccharide cleavage inhibitor 12.
【0018】[製造例13]タラゴンの生葉1Kgに3
0lの50%エタノール水溶液を加え、2時間リフラッ
クスさせた。濾過した後濃縮し蒸留水で溶解分散させ、
蒸留水で充填したダイアイオンHP−20にチャージし
1lの水で水洗し、20%エタノール水溶液2lを流し
て溶出させ、これを減圧溜去し21gのムコ多糖類切断
抑制剤13を得た。[Production Example 13] 3 kg per 1 kg of fresh leaves of tarragon
0 l of a 50% aqueous ethanol solution was added and refluxed for 2 hours. After filtration, concentrated, dissolved and dispersed in distilled water,
DIAION HP-20 charged with distilled water was charged, washed with 1 L of water, and eluted by flowing 2 L of a 20% aqueous ethanol solution, and distilled under reduced pressure to obtain 21 g of mucopolysaccharide cleavage inhibitor 13.
【0019】[製造例14]ローズマリーの生葉1Kg
に30lの50%エタノール水溶液を加え、2時間リフ
ラックスさせた。濾過した後濃縮し蒸留水で溶解分散さ
せ、蒸留水で充填したダイアイオンHP−20にチャー
ジし1lの水で水洗し、20%エタノール水溶液2lを
流して溶出させ、これを減圧溜去し21gのムコ多糖類
切断抑制剤14を得た。[Production Example 14] 1 kg of fresh leaves of rosemary
Then, 30 l of a 50% aqueous ethanol solution was added thereto, and the mixture was refluxed for 2 hours. After filtration, the mixture is concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water, and eluted by flowing 2 l of a 20% aqueous ethanol solution. The mucopolysaccharide cleavage inhibitor 14 was obtained.
【0020】[製造例15]ラベンダーの生葉1Kgに
30lの50%エタノール水溶液を加え、2時間リフラ
ックスさせた。濾過した後濃縮し蒸留水で溶解分散さ
せ、蒸留水で充填したダイアイオンHP−20にチャー
ジし1lの水で水洗し、20%エタノール水溶液2lを
流して溶出させ、これを減圧溜去し21gのムコ多糖類
切断抑制剤15を得た。[Production Example 15] To 1 kg of fresh lavender leaves, 30 l of a 50% aqueous ethanol solution was added and refluxed for 2 hours. After filtration, the mixture is concentrated, dissolved and dispersed in distilled water, charged into DIAION HP-20 filled with distilled water, washed with 1 l of water, and eluted by flowing 2 l of a 20% aqueous ethanol solution. The mucopolysaccharide cleavage inhibitor 15 was obtained.
【0021】[製造例16]スペアミントの生葉1Kg
に30lの50%エタノール水溶液を加え、2時間リフ
ラックスさせた。濾過した後濃縮し54gのムコ多糖類
切断抑制剤16を得た。[Production Example 16] 1 kg of fresh spearmint leaves
Then, 30 l of a 50% aqueous ethanol solution was added thereto, and the mixture was refluxed for 2 hours. After filtration, the filtrate was concentrated to obtain 54 g of mucopolysaccharide cleavage inhibitor 16.
【0022】[製造例17]サルビア・オフィシナリス
・バールラチフォリアの生葉1Kgに10lの1,3−
ブタンジオールを加え90℃で3時間加熱し濾過して
9.8lのムコ多糖類切断抑制剤17を得た。[Preparation Example 17] 1 l of fresh leaves of Salvia officinalis varlatifolia was added with 10 l of 1,3-
Butanediol was added, the mixture was heated at 90 ° C. for 3 hours and filtered to obtain 9.8 l of mucopolysaccharide cleavage inhibitor 17.
【0023】[試験例]ムコ多糖類を切断する作用を有
することが知られている2,2’−アゾビス(2−アミ
ジノプロパン)ハイドクロライド(以下、AAPH)を
検体とともにヒアルロン酸ナトリウム水溶液に加え、イ
ンキュベートし、切断されたヒアルロン酸をアルブミン
と沈澱させこれを600nmの吸光光度で濁度として測
定した。即ち、0.04%ヒアルロン酸0.3M燐酸緩
衝液(pH5.3)溶液1mlに試料1mgを溶かした
0.3M燐酸緩衝液(pH5.3)100μlと250
mMのAAPH0.3M燐酸緩衝液(pH5.3)溶液
200μlを加え37℃で18時間インキュベートし
た。これから200μlをサンプリングし0.1%アル
ブミン0.3M酢酸緩衝液(pH3.75)溶液2ml
を加え、吸光光度計で600nmの吸光度を測定した。
この吸光度より、次の式1に従ってムコ多糖類切断抑制
活性を算出した。[Test Example] 2,2′-azobis (2-amidinopropane) hydride (hereinafter, AAPH), which is known to have an action of cleaving mucopolysaccharide, is added to a sodium hyaluronate aqueous solution together with a sample. After incubation, the cleaved hyaluronic acid was precipitated with albumin, and this was measured as turbidity by absorbance at 600 nm. That is, 100 μl of 0.3 M phosphate buffer (pH 5.3) obtained by dissolving 1 mg of a sample in 1 ml of a 0.04% hyaluronic acid 0.3 M phosphate buffer (pH 5.3) and 250
200 μl of a mM AAPH 0.3 M phosphate buffer (pH 5.3) solution was added, and the mixture was incubated at 37 ° C. for 18 hours. From this, 200 µl was sampled and 2 ml of 0.1% albumin 0.3 M acetate buffer (pH 3.75) solution
Was added, and the absorbance at 600 nm was measured with an absorptiometer.
From this absorbance, the mucopolysaccharide cleavage inhibitory activity was calculated according to the following equation 1.
【0024】ムコ多糖類切断抑制活性(%)=(吸光度
−0.015)×182 式1Mucopolysaccharide cleavage inhibitory activity (%) = (absorbance−0.015) × 182 Formula 1
【0025】[0025]
【表1】 [Table 1]
【0026】(2)本発明の組成物 本発明の組成物は上記ムコ多糖類切断抑制剤を含有する
ことを特徴とする。本発明の組成物は、ヒアルロン酸に
代表されるムコ多糖類が化学物質や光、物理的摩擦によ
って切断されるのを抑制する作用を有する。例えば、強
い光により体内の酸素や水から生じた一重項の酸素や種
々のラジカルによって誘導された活性化学物質によるム
コ多糖類の架橋反応や切断反応、関節の過度の使用に起
因する摩擦によるムコ多糖類の切断等からの保護組成物
として用いることが出来る。又、リューマチ等の治療に
於いてヒアルロン酸等と関節に投与すれば、投与したヒ
アルロン酸などのムコ多糖類が切断されずに有効に効果
を発揮することが出来る。即ち、本発明の組成物はムコ
多糖類の切断による疾病の治療用又は予防用の化粧料、
食品、医薬品などが例示できる。本発明の組成物は上記
ムコ多糖類切断抑制剤以外にこれらの製剤で通常用いら
れている任意成分を含有することが出来る。かかる任意
成分としては、化粧品であれば、ワセリンやマイクロク
リスタリンワックス等のような炭化水素類、ホホバ油や
ゲイロウ等のエステル類、牛脂、オリーブ油等のトリグ
リセライド類、セタノール、オレイルアルコール等の高
級アルコール類、ステアリン酸、オレイン酸等の脂肪
酸、グリセリンや1,3−ブタンジオール等の多価アル
コール類、非イオン界面活性剤、アニオン界面活性剤、
カチオン界面活性剤、両性界面活性剤、エタノール、カ
ーボポール等の増粘剤、防腐剤、紫外線吸収剤、抗酸化
剤、色素、粉体類等が例示でき、医薬品であれば、賦形
剤、結合剤、被覆剤、滑沢剤、糖衣剤、崩壊剤、増量
剤、矯味矯臭剤、乳化・可溶化・分散剤、安定剤、pH
調整剤、等張剤等が例示でき、食品であれば保存料、乳
化剤、安定剤、増粘剤、矯味矯臭剤、呈味剤、甘味剤、
酸味剤、着色料等が例示できる。本発明の組成物に於け
るムコ多糖類切断抑制剤の好ましい含有量は、0.01
〜20重量%であり、より好ましくは0.05〜10重
量%であり、更に好ましくは0.1〜5重量%である。
又、医薬品に於ける好ましいムコ多糖類切断抑制剤の投
与量は成人1人1日あたり、0.1〜10000mgが
好ましい。ムコ多糖類切断抑制剤はこの量を一度に投与
することもできるし、数回に分けて投与することも可能
である。これは本発明のムコ多糖類切断抑制剤がハーブ
を基源植物としているので安全性に優れるためである。(2) Composition of the Present Invention The composition of the present invention is characterized by containing the above-mentioned mucopolysaccharide cleavage inhibitor. The composition of the present invention has an action of suppressing the mucopolysaccharide represented by hyaluronic acid from being cut by a chemical substance, light, or physical friction. For example, mucopolysaccharide cross-linking and cleavage reactions by active chemicals induced by singlet oxygen and various radicals generated from oxygen and water in the body due to strong light, and muco due to friction caused by excessive use of joints It can be used as a composition for protecting polysaccharides from cleavage. Also, when administered to a joint with hyaluronic acid or the like in the treatment of rheumatism or the like, the administered mucopolysaccharides such as hyaluronic acid can be effectively exerted without being cleaved. That is, the composition of the present invention is a cosmetic for treating or preventing a disease caused by cleavage of mucopolysaccharide,
Foods, pharmaceuticals and the like can be exemplified. The composition of the present invention can contain, in addition to the above-mentioned mucopolysaccharide cleavage inhibitor, optional components commonly used in these preparations. As such optional components, in the case of cosmetics, hydrocarbons such as petrolatum and microcrystalline wax, esters such as jojoba oil and gay wax, triglycerides such as tallow, olive oil, and higher alcohols such as cetanol and oleyl alcohol , Stearic acid, fatty acids such as oleic acid, polyhydric alcohols such as glycerin and 1,3-butanediol, nonionic surfactants, anionic surfactants,
Cationic surfactants, amphoteric surfactants, ethanol, thickeners such as carbopol, preservatives, ultraviolet absorbers, antioxidants, pigments, powders and the like can be exemplified, if a pharmaceutical, excipients, Binders, coating agents, lubricants, sugar coatings, disintegrants, bulking agents, flavoring agents, emulsifying / solubilizing / dispersing agents, stabilizers, pH
Modifiers, isotonic agents and the like can be exemplified, if food, preservatives, emulsifiers, stabilizers, thickeners, flavoring agents, flavoring agents, sweeteners,
Sour agents, coloring agents and the like can be exemplified. The preferred content of the mucopolysaccharide cleavage inhibitor in the composition of the present invention is 0.01
-20% by weight, more preferably 0.05-10% by weight, even more preferably 0.1-5% by weight.
The dosage of the preferred mucopolysaccharide cleavage inhibitor in pharmaceuticals is preferably from 0.1 to 10,000 mg per adult per day. This amount of the mucopolysaccharide cleavage inhibitor can be administered at once, or can be administered in several divided doses. This is because the mucopolysaccharide cleavage inhibitor of the present invention is excellent in safety since herbs are used as base plants.
【0027】[0027]
【実施例】以下に実施例を挙げて本発明について詳細に
説明するが、本発明がこれらの実施例にのみ限定を受け
ないことは言うまでもない。尚、数値は重量部を表す。EXAMPLES The present invention will be described in detail below with reference to examples, but it goes without saying that the present invention is not limited to only these examples. The numerical values represent parts by weight.
【0028】実施例1〜4 配合例 表2に示す処方で化粧水を作成した。即ち、処方成分を
室温で攪拌可溶化して化粧水を得た。Examples 1-4 Formulation Examples Lotions were prepared according to the formulations shown in Table 2. That is, the ingredients were stirred and solubilized at room temperature to obtain a lotion.
【0029】[0029]
【表2】 [Table 2]
【0030】実施例5〜8 配合例 表3に示す処方で化粧水を作成した。即ち、処方成分を
室温で攪拌可溶化して化粧水を得た。Examples 5 to 8 Formulation Examples A lotion was prepared according to the formulation shown in Table 3. That is, the ingredients were stirred and solubilized at room temperature to obtain a lotion.
【0031】[0031]
【表3】 [Table 3]
【0032】実施例9〜11 配合例 下記表4に示す処方に従ってクリームを作成した。即
ち、イ、ロ、ハをそれぞれ80℃に加熱溶解して、イに
ロを徐々に加え、更にハを加え乳化した後、ホモミキサ
ーにより乳化粒子を均一化し、冷却してクリームを得
た。Examples 9 to 11 Formulation Examples Creams were prepared according to the formulations shown in Table 4 below. That is, (a), (b) and (c) were each heated and dissolved at 80 ° C., (b) was gradually added to (a), and (c) was further added and emulsified. The homogenized particles were homogenized by a homomixer and cooled to obtain a cream.
【0033】[0033]
【表4】 [Table 4]
【0034】実施例12 配合例 下記の処方に従ってファンデーションを作成した。即
ち、イ、ロ、ハをそれぞれ80℃で加熱溶解させ、イを
良く混練りし、これをロで希釈し、これにニを分散さ
せ、ハを徐々に加え乳化し攪拌冷却してファンデーショ
ンを得た。 イ マルチトール70%水溶液 10 1,3−ブタンジオール 5 メチルパラベン 0.3 トリグリセリンジイソステアレート 4 ロ 流動パラフィン 16 軽質流動イソパラフィン 4 ブチルパラベン 0.1 ハ 水 40.6 ニ 酸化チタン 10 タルク 5 黄色酸化鉄 3.5 ベンガラ 0.5 ムコ多糖類切断抑制剤12 0.5 ムコ多糖類切断抑制剤13 0.5Example 12 Formulation Example A foundation was prepared according to the following formulation. That is, a, b, and c are each heated and melted at 80 ° C, kneaded well with b, diluted with b, dispersed in d, gradually added with c, emulsified, stirred and cooled to form a foundation. Obtained. A 70% aqueous solution of maltitol 10 1,3-butanediol 5 methyl paraben 0.3 triglycerin diisostearate 4 b liquid paraffin 16 light liquid isoparaffin 4 butyl paraben 0.1 c water 40.6 titanium dioxide 10 talc 5 yellow Iron oxide 3.5 Bengala 0.5 Mucopolysaccharide cleavage inhibitor 12 0.5 Mucopolysaccharide cleavage inhibitor 13 0.5
【0035】実施例13 配合例 下記処方に従ってヘアトニックを作成した。即ち、処方
成分を室温で攪拌可溶化してヘアトニックを得た。 トウガラシチンキ 0.1 エチニルエストラジオール 0.1 メントール 0.1 香料 0.1 ムコ多糖類切断抑制剤14 0.1 エタノール 60 水 39.5Example 13 Formulation Example A hair tonic was prepared according to the following formulation. That is, the ingredients were stirred and solubilized at room temperature to obtain a hair tonic. Pepper tincture 0.1 Ethinyl estradiol 0.1 Menthol 0.1 Fragrance 0.1 Mucopolysaccharide cleavage inhibitor 14 0.1 Ethanol 60 Water 39.5
【0036】実施例14 配合例 下記処方に従ってパンを作成した。即ち、処方成分を良
く混練りし、37℃で45分一次発酵させ、成形した後
30分二次発酵させ、200℃のオーブンで30分焼い
てパンを得た。このパンは作成後1週間でも風味が落ち
なかった。 強力粉 500 砂糖 30 バター 50 塩 5 ドライイースト 12 ムコ多糖類切断抑制剤15 3 水 250Example 14 Formulation Example Bread was prepared according to the following formulation. That is, the ingredients were kneaded well, subjected to primary fermentation at 37 ° C. for 45 minutes, formed, then subjected to secondary fermentation for 30 minutes, and baked in a 200 ° C. oven for 30 minutes to obtain bread. This bread did not lose its flavor even one week after it was made. Strong flour 500 Sugar 30 Butter 50 Salt 5 Dry yeast 12 Mucopolysaccharide cutting inhibitor 15 3 Water 250
【0037】実施例15〜17 配合例 下記表5の処方に従ってキャンディーを作成した。即
ち、処方成分を120℃で加熱溶解し110℃まで冷ま
し、型に流し込んで固化させて、型から外しキャンディ
ーを得た。Examples 15-17 Formulation Examples Candies were prepared according to the formulation shown in Table 5 below. That is, the ingredients were heated and dissolved at 120 ° C., cooled to 110 ° C., poured into a mold and solidified, and removed from the mold to obtain a candy.
【0038】[0038]
【表5】 [Table 5]
【0039】実施例18 配合例 下記処方に従ってクッキーを作成した。即ち、バターと
砂糖とを良く混練りし、これに卵黄を加え良く混ぜ、更
に薄力粉とベーキングパウダーとアーモンドプードルと
ムコ多糖類切断抑制剤2を加えさっくりまぜ、成形して
180℃のオーブンで30分焼きクッキーを得た。この
クッキーは1ヶ月立ってもバターの風味が損なわれなか
った。 薄力粉 150 アーモンドプードル 50 砂糖 80 バター 50 卵黄 2個分 ベーキングパウダー 1 ムコ多糖類切断抑制剤8 1Example 18 Formulation Example A cookie was prepared according to the following recipe. That is, knead butter and sugar well, add egg yolk to this, mix well, further add flour, baking powder, almond poodle and mucopolysaccharide cutting inhibitor 2, mix and shape, and in a 180 ° C oven A 30 minute baked cookie was obtained. This cookie did not lose its buttery flavor after standing for one month. Light flour 150 Almond poodle 50 Sugar 80 Butter 50 Egg yolk 2 baking powder 1 Mucopolysaccharide cutting inhibitor 8 1
【0040】実施例19 配合例 下記処方に従って顆粒剤を作成した。即ち、処方成分を
グラッド造粒機で良く混ぜ、これに50%エタノール水
溶液50重量部を噴霧して加え、造粒した後、40℃で
48時間送風乾燥し、篩過して顆粒剤を得た。 乳糖 50 結晶セルロース 40 ヒドロキシプロピルセルロース 5 ムコ多糖類切断抑制剤4 5Example 19 Formulation Example A granule was prepared according to the following formulation. That is, the prescription components are mixed well by a Glad granulator, and 50 parts by weight of a 50% aqueous ethanol solution is sprayed and added thereto. After granulation, the mixture is blown dry at 40 ° C. for 48 hours and sieved to obtain granules. Was. Lactose 50 Crystalline cellulose 40 Hydroxypropyl cellulose 5 Mucopolysaccharide cleavage inhibitor 45
【0041】実施例20 配合例 下記の処方に従って皮膚外用剤を作成した。即ち、処方
成分を良く混練りし皮膚外用剤を得た。 ワセリン 90 ムコ多糖類切断抑制剤8 10Example 20 Formulation Example An external preparation for skin was prepared according to the following formulation. That is, the prescription components were well kneaded to obtain a skin external preparation. Vaseline 90 Mucopolysaccharide cleavage inhibitor 8 10
【0042】[0042]
【発明の効果】本発明のムコ多糖類切断抑制剤はムコ多
糖類が切断されるのを抑制する作用に優れるので、これ
を含有する化粧料、食品、医薬品等の組成物を投与する
ことにより容易にムコ多糖類の切断を抑制しうる。The mucopolysaccharide cleavage inhibitor of the present invention is excellent in the action of inhibiting the cleavage of mucopolysaccharide. Therefore, by administering a composition containing the same to cosmetics, foods, pharmaceuticals, etc. It can easily suppress cleavage of mucopolysaccharide.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 FI 技術表示箇所 A23G 3/00 101 A23G 3/00 101 102 102 A23L 1/221 A23L 1/221 C 1/30 1/30 A A61K 7/00 A61K 7/00 K 7/02 7/02 Z 7/06 7/06 7/48 7/48 (72)発明者 岡田 正紀 神奈川県横浜市神奈川区高島台27番地1 ポーラ化成工業株式会社横浜研究所内──────────────────────────────────────────────────続 き Continuation of the front page (51) Int.Cl. 6 Identification number Office reference number FI Technical display location A23G 3/00 101 A23G 3/00 101 102 102 A23L 1/221 A23L 1/221 C 1/30 1 / 30 A A61K 7/00 A61K 7/00 K 7/02 7/02 Z 7/06 7/06 7/48 7/48 (72) Inventor Masaki Okada 27-1, Takashimadai, Kanagawa-ku, Yokohama, Kanagawa, Japan Kasei Kogyo Co., Ltd.
Claims (9)
糖類切断抑制剤。1. A mucopolysaccharide cleavage inhibitor comprising an essence of a Labiatae plant.
ミント、ウォーターミント、日本ハッカ、、セージ、ロ
ーズマリー、マジョラム、キャットニップ、ラベンダ
ー、タイム、タラゴン、レモンバームの何れかである、
請求項1記載のムコ多糖類切断抑制剤。2. The Labiatae plant is any of spearmint, peppermint, water mint, Japanese peppermint, sage, rosemary, marjoram, catnip, lavender, thyme, tarragon, and lemon balm.
The mucopolysaccharide cleavage inhibitor according to claim 1.
制剤を含有するムコ多糖類切断抑制用の組成物。3. A composition for inhibiting mucopolysaccharide cleavage, comprising the mucopolysaccharide cleavage inhibitor according to claim 1 or 2.
物。4. The composition according to claim 3, wherein the use is a medicament.
成物。5. The composition according to claim 3, wherein the composition is used for cosmetics.
物。6. The composition according to claim 3, wherein the composition is used for food.
コ多糖類切断抑制用の化粧料。7. A cosmetic composition for inhibiting mucopolysaccharide cleavage, which contains an essence of a Labiatae plant.
ント、ウォーターミント、日本ハッカ、セージ、ローズ
マリー、マジョラム、キャットニップ、ラベンダー、タ
イム、タラゴン、レモンバームの何れかである、請求項
7に記載の化粧料。8. The cosmetic according to claim 7, wherein the Labiatae plant is any of spearmint, peppermint, water mint, Japanese peppermint, sage, rosemary, marjoram, catnip, lavender, thyme, tarragon, and lemon balm. .
断抑制のための使用。9. Use of an essence of a Labiatae plant for inhibiting mucopolysaccharide cleavage.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8207980A JPH1036282A (en) | 1996-07-18 | 1996-07-18 | Agent for suppressing breakage of mucopolysaccharide |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8207980A JPH1036282A (en) | 1996-07-18 | 1996-07-18 | Agent for suppressing breakage of mucopolysaccharide |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH1036282A true JPH1036282A (en) | 1998-02-10 |
Family
ID=16548693
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8207980A Pending JPH1036282A (en) | 1996-07-18 | 1996-07-18 | Agent for suppressing breakage of mucopolysaccharide |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH1036282A (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001019628A (en) * | 1999-07-06 | 2001-01-23 | Pola Chem Ind Inc | Cosmetic material for retaining and improving shape |
JP2001122777A (en) * | 1999-10-27 | 2001-05-08 | Nagase & Co Ltd | Antiulcer agent |
JP2003002820A (en) * | 2001-06-22 | 2003-01-08 | Naris Cosmetics Co Ltd | Skin care composition |
JP2004083476A (en) * | 2002-08-27 | 2004-03-18 | Horin:Kk | Tyrosinase inhibitor, demelanizing agent, fibroblast reproductive factor and estrogenic substance, and skin cosmetic |
JP2005505544A (en) * | 2001-08-31 | 2005-02-24 | ラトガーズ, ザ ステイト ユニバーシティ オブ ニュー ジャージー | Method for treating disorders using plant extracts |
JP2005206533A (en) * | 2004-01-23 | 2005-08-04 | Fancl Corp | Composition for inhibiting vascularization |
JP2010510267A (en) * | 2006-11-24 | 2010-04-02 | ディーエスエム アイピー アセッツ ビー.ブイ. | Composition comprising carnosic acid 12-methyl ether |
JP2012136473A (en) * | 2010-12-27 | 2012-07-19 | Maruzen Pharmaceut Co Ltd | Hyaluronic acid production promoter |
JP2015042675A (en) * | 2014-11-13 | 2015-03-05 | 株式会社再春館製薬所 | Expression inducer for heat shock protein |
-
1996
- 1996-07-18 JP JP8207980A patent/JPH1036282A/en active Pending
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001019628A (en) * | 1999-07-06 | 2001-01-23 | Pola Chem Ind Inc | Cosmetic material for retaining and improving shape |
JP2001122777A (en) * | 1999-10-27 | 2001-05-08 | Nagase & Co Ltd | Antiulcer agent |
JP2003002820A (en) * | 2001-06-22 | 2003-01-08 | Naris Cosmetics Co Ltd | Skin care composition |
JP2005505544A (en) * | 2001-08-31 | 2005-02-24 | ラトガーズ, ザ ステイト ユニバーシティ オブ ニュー ジャージー | Method for treating disorders using plant extracts |
JP4744081B2 (en) * | 2001-08-31 | 2011-08-10 | ラトガーズ, ザ ステイト ユニバーシティ オブ ニュー ジャージー | Method for treating disorders using plant extracts |
JP2004083476A (en) * | 2002-08-27 | 2004-03-18 | Horin:Kk | Tyrosinase inhibitor, demelanizing agent, fibroblast reproductive factor and estrogenic substance, and skin cosmetic |
JP2005206533A (en) * | 2004-01-23 | 2005-08-04 | Fancl Corp | Composition for inhibiting vascularization |
JP2010510267A (en) * | 2006-11-24 | 2010-04-02 | ディーエスエム アイピー アセッツ ビー.ブイ. | Composition comprising carnosic acid 12-methyl ether |
JP2010510266A (en) * | 2006-11-24 | 2010-04-02 | ディーエスエム アイピー アセッツ ビー.ブイ. | Use of tricyclic diterpenes and their derivatives for the treatment, co-treatment or prevention of inflammatory and / or joint disorders |
JP2013255495A (en) * | 2006-11-24 | 2013-12-26 | Dsm Ip Assets Bv | Composition comprising carnosic acid 12-methylether |
JP2012136473A (en) * | 2010-12-27 | 2012-07-19 | Maruzen Pharmaceut Co Ltd | Hyaluronic acid production promoter |
JP2015042675A (en) * | 2014-11-13 | 2015-03-05 | 株式会社再春館製薬所 | Expression inducer for heat shock protein |
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