JPH10271987A - Bacterium-examination medium packed in bag - Google Patents
Bacterium-examination medium packed in bagInfo
- Publication number
- JPH10271987A JPH10271987A JP9810397A JP9810397A JPH10271987A JP H10271987 A JPH10271987 A JP H10271987A JP 9810397 A JP9810397 A JP 9810397A JP 9810397 A JP9810397 A JP 9810397A JP H10271987 A JPH10271987 A JP H10271987A
- Authority
- JP
- Japan
- Prior art keywords
- bag
- medium
- closing means
- gas
- opening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】この発明は、食品や血液など
の検体に含まれる細菌を培養するために袋に入れられた
培地に属する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a medium packed in a bag for culturing bacteria contained in a specimen such as food or blood.
【０００２】[0002]
【従来の技術】従来の細菌培養に用いる容器は、ガラス
や硬質樹脂等の剛性材料からなる定形のシャーレ、ボト
ル又は試験管であった。それぞれの容器の使用方法は、
例えば以下のようである。2. Description of the Related Art Conventional containers used for culturing bacteria have been fixed petri dishes, bottles or test tubes made of a rigid material such as glass or hard resin. How to use each container,
For example, it is as follows.
【０００３】シャーレで培養する場合、検体（通常、好
気性菌）をピペットで滅菌シャーレに入れ、次いで寒天
培地を加え、蓋を被せて検体や培地がこぼれないように
注意しながら直ちにシャーレを振って十分混釈する。そ
の後、好気的条件下の恒温装置内で培養する。[0003] When culturing in a petri dish, a specimen (usually an aerobic bacterium) is pipetted into a sterile petri dish, and then an agar medium is added. Pour enough. Thereafter, the cells are cultured in a thermostat under aerobic conditions.
【０００４】試験管で培養する場合、試験管Ｔの中に細
菌の増殖とともにガスを発生する液体培地Ｍを充填し、
望ましくはその培地Ｍに試験管Ｔよりもはるかに小さい
ダーラム管Ｄを空気を抜いて逆さに沈めておく。細菌が
存在するときは、培養とともに発生したガスＧがダーラ
ム管Ｄ内に溜まる。こうして、ガス発生の有無をもって
細菌の存否を確認する。ダーラム管を沈めない代わりに
培地から目をそらすことなく、注意深くガス発生を観察
してもよい。When culturing in a test tube, a test tube T is filled with a liquid medium M that generates gas with the growth of bacteria,
Desirably, a Durham tube D, which is much smaller than the test tube T, is evacuated and submerged in the medium M upside down. When bacteria are present, the gas G generated during the culture accumulates in the Durham tube D. Thus, the presence or absence of bacteria is confirmed by the presence or absence of gas generation. Careful observation of gas evolution may be made without distracting the Durham tube, instead of looking away from the medium.
【０００５】一方、ボトルで培養する場合、試験管培養
と同様にダーラム管を逆さに沈めてガス発生の状況を観
察するか、又は細菌の増殖とともに色が変わる培地を充
填し、変色の程度によって細菌の存否を確認する。[0005] On the other hand, when culturing in a bottle, the durham tube is sunk upside down to observe the state of gas generation as in the case of test tube culture, or a medium that changes color with the growth of bacteria is filled, and depending on the degree of color change. Check for the presence of bacteria.
【０００６】[0006]
【発明が解決しようとする課題】しかし、シャーレは完
全密封構造でないので、無菌状態で保存又は輸送するに
は無菌袋に入れる必要がある。また、シャーレであろう
と試験管であろうとボトルであろうと、脆性材料である
ので、保存や輸送の際には衝撃吸収材とともに梱包する
必要があり、大きな体積を占有する。それ故、この発明
の目的は、小さな占有体積で保存・輸送可能な細菌検査
用培地を提供することにある。However, since the petri dish is not completely sealed, it is necessary to put it in a sterile bag to store or transport it in a sterile state. Further, whether it is a petri dish, a test tube, or a bottle, it is a brittle material, so it needs to be packed together with a shock absorbing material during storage and transportation, and occupies a large volume. Therefore, an object of the present invention is to provide a culture medium for bacterial test which can be stored and transported in a small occupied volume.
【０００７】[0007]
【課題を解決するための手段】上記目的を達成するため
に、この発明の細菌検査用培地は、培地と、柔軟性材料
からなり、一側に開閉手段を有するとともに、開閉手段
の外側で閉塞し、培地が充填された袋と、を備えたこと
を特徴とする。In order to achieve the above object, a culture medium for bacteria test of the present invention is composed of a culture medium and a flexible material, has an opening / closing means on one side, and is closed outside the opening / closing means. And a bag filled with a culture medium.
【０００８】この発明によれば、開閉手段を有する袋に
通常は無菌状態の培地が充填されているが、開閉手段の
外側で閉塞しているので、保存中及び輸送中も無菌状態
が維持される。袋が柔軟性材料からなるので、自在に体
積や形状を変化させることができ、占有体積が少なくて
済む。According to the present invention, the bag having the opening / closing means is normally filled with a sterile medium, but since the medium is closed outside the opening / closing means, the sterile state is maintained during storage and transportation. You. Since the bag is made of a flexible material, the volume and shape can be freely changed, and the occupied volume can be reduced.
【０００９】使用時にはハサミなどで開閉手段側の端部
と開閉手段との間を切り、開閉手段を開けて検体を加え
る。開閉手段を閉じ、開閉手段側端部を上にして培養す
る。従って、上記の袋は、使用前は培地供給容器、使用
中は培養容器となり、一つの袋を兼用できる。In use, the end between the opening and closing means and the opening and closing means are cut off with scissors or the like, and the opening and closing means is opened to add a sample. The culture is performed with the opening / closing means closed and the end on the opening / closing means side up. Therefore, the above bag becomes a culture medium supply container before use and a culture container during use, and one bag can also be used.
【００１０】[0010]
【発明の実施の形態】袋を構成する柔軟性材料は、培養
する細菌によって適宜選択すればよい。例えば好気性菌
の培養には、ポリ塩化ビニル、ポリエチレン等の通気性
のある合成樹脂フィルムが好ましく、嫌気性菌の培養に
は、単独で気体不透過性を発揮するフィルムか又は重層
フィルムが好ましい。BEST MODE FOR CARRYING OUT THE INVENTION The flexible material constituting the bag may be appropriately selected depending on the bacteria to be cultured. For example, for cultivation of aerobic bacteria, a breathable synthetic resin film such as polyvinyl chloride and polyethylene is preferable, and for cultivation of anaerobic bacteria, a film exhibiting gas impermeability alone or a multilayer film is preferable. .
【００１１】開閉手段は、例えばファスナーのように一
側に沿って形成された可撓性の凹部と対向側に沿って形
成された可撓性の凸部とからなり、凹部と凸部との嵌合
によって閉じるものである。The opening / closing means comprises a flexible concave portion formed along one side, such as a fastener, and a flexible convex portion formed along the opposite side. It is closed by fitting.
【００１２】細菌の増殖とともにガスが発生する培地を
用いる場合、培地にガスが発生したとき、そのガスを溜
めるガストラッパーを本発明検体容器の内部に一体的に
設けることで、ガス発生を容易に確認できる。ガストラ
ッパーは、開口部に向かって狭まる空間からなるもので
あればよい。In the case of using a medium in which gas is generated with the growth of bacteria, when a gas is generated in the medium, a gas trapper for storing the gas is integrally provided inside the sample container of the present invention, so that the gas can be easily generated. You can check. The gas trapper may be formed of a space narrowing toward the opening.
【００１３】ガストラッパーと開閉手段材との間には、
ガストラッパーに溜まったガスを開閉手段材の位置まで
案内するガイドが存在すると好ましい。検体を袋内の培
地に接種する際に開閉手段材から空気が混入するが、そ
のまま培養するとガストラッパーに溜まっているガスが
細菌増殖に由来するものであるのか空気であるのか区別
できない。ガイドが存在すれば、混入した空気をガイド
に沿って速やかに排出することができるからである。[0013] Between the gas trapper and the opening and closing means material,
It is preferable that there is a guide for guiding the gas accumulated in the gas trapper to the position of the opening / closing member. When the sample is inoculated into the culture medium in the bag, air is mixed in from the opening / closing means. However, if the sample is cultured as it is, it cannot be distinguished whether the gas accumulated in the gas trapper is derived from bacterial growth or air. This is because if the guide exists, the mixed air can be quickly discharged along the guide.
【００１４】袋の形状は限定されないが、平面視でフラ
スコの正面と同一形状にすると、例えばパウチ法でウェ
ルシュ菌を検査する場合に、シャーレと同様に円形の視
野内で菌数を数えることができる。Although the shape of the bag is not limited, if the shape of the bag is the same as the front of the flask in plan view, the number of bacteria can be counted in a circular visual field in the same manner as in a petri dish, for example, when testing for Welsh bacteria by the pouch method. it can.
【００１５】[0015]
−実施例１− この発明の第一の実施例を図面とともに説明する。図１
は水を検査するための袋入り細菌検査用培地の第一実施
例を示す斜視図、図２は図１のＸＹ断面図である。-Embodiment 1- A first embodiment of the present invention will be described with reference to the drawings. FIG.
1 is a perspective view showing a first embodiment of a bag-containing bacterial test medium for testing water, and FIG. 2 is an XY sectional view of FIG.
【００１６】袋入り細菌検査用培地１は、袋２と粉末状
の無菌培地３とからなる。袋２は、無色透明の２枚のポ
リエチレン製方形フィルム２ａ，２ｂを周辺部２ｃで互
いに熱圧着等により接着して得られたものである。袋２
は頭部側に袋２と一体的に開閉手段としてのファスナー
４を有する。ファスナー４は、頭部端縁に沿って形成さ
れた一対の可撓性の凹部４１及び凸部４２からなる。凹
部４１及び凸部４２は、断面円形で互いに相補する形状
をなし、噛み合いによって弾力的に閉じている。培地３
は、袋２の底部より入れられ、底部を熱圧着し完全密閉
した後、放射線で滅菌されたＬＭＸブロス粉末（メルク
社製）ものである。The bag-containing culture medium for bacterial test 1 comprises a bag 2 and a powdered sterile medium 3. The bag 2 is obtained by bonding two colorless and transparent polyethylene square films 2a and 2b to each other at a peripheral portion 2c by thermocompression bonding or the like. Bag 2
Has a fastener 4 as opening / closing means integrally with the bag 2 on the head side. The fastener 4 includes a pair of flexible concave and convex portions 41 and 42 formed along the edge of the head. The concave portion 41 and the convex portion 42 have a circular cross section and have mutually complementary shapes, and are elastically closed by meshing. Medium 3
Is an LMX broth powder (manufactured by Merck) which is put in from the bottom of the bag 2, heat-pressed and completely sealed, and then sterilized with radiation.
【００１７】袋２は、全体がポリエチレン製であるか
ら、培地３を充填する前は不使用時には多数個を平たく
束ねたり折り曲げたりして、保管場所に応じて適当な形
態で保存することができ、運搬も容易である。しかも運
搬中、破損の心配もなく、軽量である。もちろん培地３
を充填後も破損の心配はない。Since the bag 2 is entirely made of polyethylene, it can be stored in an appropriate form depending on the storage place by bundling or bending a large number of them when not in use before filling the medium 3. Easy to transport. In addition, there is no risk of breakage during transportation, and it is lightweight. Medium 3
There is no worry about damage after filling.
【００１８】使用時にはファスナー４の上部を切り取
り、ファスナー４を開け、検水を入れ、ファスナー４を
閉じる。粉末培地３を溶かす。袋２をラックに掛ける又
は吊り下げるなどして立てて３５−３７℃にて培養し、
２４−４８時間後に判定する。袋２が透明であるから袋
２を透かして培地の色の変化を観察することができる。
大腸菌群の存在は培地色が淡黄色より青色に変化するこ
とにより、また大腸菌の存在は紫外線ランプ照射により
蛍光を発することにより判定できる。At the time of use, the upper part of the fastener 4 is cut off, the fastener 4 is opened, water is sampled, and the fastener 4 is closed. Dissolve powder medium 3. Hang the bag 2 on a rack or hang it up, and cultivate it at 35-37 ° C.
Determine after 24-48 hours. Since the bag 2 is transparent, a change in the color of the culture medium can be observed through the bag 2.
The presence of Escherichia coli can be determined by changing the medium color from pale yellow to blue, and the presence of Escherichia coli can be determined by emitting fluorescence by irradiation with an ultraviolet lamp.
【００１９】本例の袋２は、フィルム２ａ，２ｂが互い
に周辺で接着されるだけでなく、両フィルムの外周の１
辺から中央に向かって延びる線６１に沿っても相互接着
されている。そして、その１辺と延線６１とで袋２の内
部に平面視で鋭角の空間を有し、それがガストラッパー
６となる。従って、袋２を立てて培養すれば、培地Ｐに
発生したガスが気泡Ｑとなって図中の矢印方向に移動し
てガストラッパー６に溜まるので、ガス発生を確認しや
すい。In the bag 2 of this embodiment, not only the films 2a and 2b are adhered to each other at the periphery, but also the outer periphery of the two films.
They are also bonded together along a line 61 extending from the side to the center. Then, one side and the extension line 61 have an acute space in a plan view inside the bag 2, and the space becomes the gas trapper 6. Therefore, if the bag 2 is raised and cultured, the gas generated in the medium P becomes bubbles Q and moves in the direction of the arrow in the figure and accumulates in the gas trapper 6, so that it is easy to confirm the gas generation.
【００２０】また、延線６１は弁３の延長上に差し掛か
ったところで方向転換して弁３に向かい、ガストラッパ
ー６に溜まったガスを弁３の位置まで案内するガイド６
２に連なる。従って、検体を袋２内の培地に接種する際
に弁３から空気が混入しても、混入した空気をガイドに
沿って培養開始前に速やかに排出することができる。そ
の結果、培養後にガストラッパー６に溜まったガスがま
さしく細菌増殖に由来するものであるとの確証を得るこ
とができる。When the extension line 61 reaches the extension of the valve 3, the direction is changed to the valve 3, and the guide 6 guides the gas accumulated in the gas trapper 6 to the position of the valve 3.
Connect to 2. Therefore, even if air enters from the valve 3 when the sample is inoculated into the culture medium in the bag 2, the mixed air can be quickly discharged along the guide before starting the culture. As a result, it can be confirmed that the gas accumulated in the gas trapper 6 after the culturing is derived from bacterial growth.
【００２１】これを確認するためにＬＭＸブロス粉末に
代えてＬＢブロス粉末を用いたこと以外は上記培地と同
一の袋入り細菌検査用培地を製造した。そして、検水注
入後直ぐに空気を追い出してファスナー４を閉じ、上記
のように袋２を立てて２４−４８時間培養した。大腸菌
群が存在すれば乳糖を分解し、ガスを産生し、これがガ
ストラッパー６に溜まるので容易に判定できる。To confirm this, a bag-containing bacterial test medium identical to the above medium was produced except that LB broth powder was used instead of LMX broth powder. Immediately after the injection of the test water, the air was expelled, the fastener 4 was closed, the bag 2 was set up as described above, and the cells were cultured for 24-48 hours. If the coliform group is present, lactose is decomposed to produce gas, which accumulates in the gas trapper 6 and can be easily determined.
【００２２】−実施例２− 図３は本発明の袋入り細菌検査用培地の第二実施例を示
す斜視図、図４は図３の断面図、図５は袋の底部を広げ
て自立させた状態を示す斜視図である。Example 2 FIG. 3 is a perspective view showing a second embodiment of the bag-containing bacterial test medium of the present invention, FIG. 4 is a sectional view of FIG. 3, and FIG. FIG.
【００２３】本例の袋１２は、ガストラッパー６が設け
られていないこと及び底部の構造においてのみ実施例１
の袋２と異なる。袋１２の底部はフィルム１２ａ，１２
ｂと異なる第三のフィルム１２ｃを二つ折りにし、その
両端縁を各々フィルム１２ａ及び１２ｂの底側端縁と熱
圧着することにより、一体化されている。本例の場合、
培地１３は、ファスナー１４側の端部より入れられ、そ
の後にファスナー１４側の端部が熱圧着される。二つ折
りにされた１２ｃを広げるだけでラックが無くとも袋１
２を立てることができるので、便利である。The bag 12 of this embodiment is different from that of the first embodiment only in that the gas trapper 6 is not provided and only in the structure of the bottom.
Different from bag 2. The bottom of the bag 12 is a film 12a, 12
The third film 12c, which is different from the film b, is folded into two, and both end edges thereof are integrated by thermocompression bonding with the bottom end edges of the films 12a and 12b, respectively. In this case,
The culture medium 13 is inserted from the end on the fastener 14 side, and then the end on the fastener 14 side is thermocompression-bonded. Even if you do not have a rack just by unfolding the folded 12c bag 1
It is convenient because 2 can be set.
【００２４】[0024]
【発明の効果】以上のように、この発明の袋入り細菌検
査用培地は、無菌状態で長期に保存することができる
し、輸送中に破損の心配がないので、便利である。As described above, the bacterial culture medium in a bag of the present invention can be stored in a sterile state for a long period of time and is convenient because there is no fear of breakage during transportation.
【図１】第一実施例の袋入り細菌検査用培地を示す斜視
図である。FIG. 1 is a perspective view showing a bag-containing bacterial test medium of a first embodiment.
【図２】図１のＸＹ断面図である。FIG. 2 is an XY cross-sectional view of FIG.
【図３】第二実施例の袋入り細菌検査用培地を示す斜視
図である。FIG. 3 is a perspective view showing a bag-containing medium for bacterial test of a second embodiment.
【図４】図２のＸＹ断面図である。FIG. 4 is an XY sectional view of FIG. 2;
【図５】第二実施例の袋入り細菌検査用培地の底部を広
げて自立させた状態を示す斜視図である。FIG. 5 is a perspective view showing a state in which the bottom of the bag-containing bacterial test medium of the second embodiment is expanded and self-standing.
１，１１ 袋入り細菌検査用培地 ２，１２ 袋 ３，１３ 無菌培地 ４，１４ ファスナー（開閉手段） ６ ガストラッパー ６１ 延線 ６２ ガイド 1,11 bags of bacteria test medium 2,12 bags 3,13 sterile medium 4,14 fasteners (opening / closing means) 6 gas trapper 61 wire extension 62 guide
Claims (4)
もに開閉手段の外側で閉塞し、培地が充填された袋とを
備えたことを特徴とする細菌検査用培地。1. A bacteria test medium, comprising: a medium, a flexible material, provided with an opening / closing means on one side, closed on the outside of the opening / closing means, and filled with a medium. .
レン等の合成樹脂である請求項１に記載の細菌検査用培
地。2. The culture medium for bacterial tests according to claim 1, wherein the flexible material is a synthetic resin such as polyvinyl chloride or polyethylene.
載の細菌検査用培地。3. The culture medium for bacterial examination according to claim 1, wherein the opening / closing means is a fastener.
く、培地が入れられた状態ではその底部が面状に広がっ
て自立可能である請求項２に記載の細菌検査用培地。4. The culture medium for bacterial examination according to claim 2, wherein the flexible material is flat before the culture medium is put therein, and the bottom portion of the flexible material is spread out in a planar manner in the state where the culture medium is put and can be self-supported.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9810397A JPH10271987A (en) | 1997-03-31 | 1997-03-31 | Bacterium-examination medium packed in bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9810397A JPH10271987A (en) | 1997-03-31 | 1997-03-31 | Bacterium-examination medium packed in bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10271987A true JPH10271987A (en) | 1998-10-13 |
Family
ID=14211002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9810397A Pending JPH10271987A (en) | 1997-03-31 | 1997-03-31 | Bacterium-examination medium packed in bag |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10271987A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006262876A (en) * | 2005-03-25 | 2006-10-05 | Nipro Corp | Culture bag, method for storing culture medium and method for culturing cell |
| JP2008536494A (en) * | 2005-04-04 | 2008-09-11 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | Flexible media bag containing nutrient concentrate |
| JP2010057390A (en) * | 2008-09-02 | 2010-03-18 | Nikken Seibutsu Igaku Kenkyusho:Kk | Test implement |
| JP2012217440A (en) * | 2011-04-14 | 2012-11-12 | Nissui Pharm Co Ltd | Bag-shaped vessel for detecting microorganism |
| KR101536705B1 (en) * | 2013-11-08 | 2015-07-14 | 건국대학교 산학협력단 | A sterilized bag comprising enrichment media, preparation method thereof and use of the same |
-
1997
- 1997-03-31 JP JP9810397A patent/JPH10271987A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006262876A (en) * | 2005-03-25 | 2006-10-05 | Nipro Corp | Culture bag, method for storing culture medium and method for culturing cell |
| JP2008536494A (en) * | 2005-04-04 | 2008-09-11 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | Flexible media bag containing nutrient concentrate |
| JP4880673B2 (en) * | 2005-04-04 | 2012-02-22 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | Flexible media bag containing nutrient concentrate |
| JP2010057390A (en) * | 2008-09-02 | 2010-03-18 | Nikken Seibutsu Igaku Kenkyusho:Kk | Test implement |
| JP2012217440A (en) * | 2011-04-14 | 2012-11-12 | Nissui Pharm Co Ltd | Bag-shaped vessel for detecting microorganism |
| KR101536705B1 (en) * | 2013-11-08 | 2015-07-14 | 건국대학교 산학협력단 | A sterilized bag comprising enrichment media, preparation method thereof and use of the same |
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