JPH0931093A - Dna salt originating from soft roe of fish and having excellent water solubility and its production - Google Patents
Dna salt originating from soft roe of fish and having excellent water solubility and its productionInfo
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- JPH0931093A JPH0931093A JP7216456A JP21645695A JPH0931093A JP H0931093 A JPH0931093 A JP H0931093A JP 7216456 A JP7216456 A JP 7216456A JP 21645695 A JP21645695 A JP 21645695A JP H0931093 A JPH0931093 A JP H0931093A
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- dna
- solution
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- water
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Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は魚類白子由来の水溶性に
優れたＤＮＡ塩類およびその製造方法に関する。詳細に
は本発明は、従来品よりも分子量が大幅に低下し、それ
によって水溶性が格段に向上し、液状食品などへの添加
が容易となるなどのメリットがある魚類白子由来の水溶
性に優れたＤＮＡアル力リ金属塩類およびその製造方法
に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a water-soluble DNA salt derived from fish milt and a method for producing the same. Specifically, the present invention has a significantly lower molecular weight than conventional products, thereby significantly improving water solubility, and has advantages such as easy addition to liquid foods and the like. The present invention relates to an excellent DNA metal salt and a method for producing the same.
【０００２】[0002]
【従来の技術】魚類の精巣、いわゆる白子からＤＮＡを
分離採取する方法としては食塩水による抽出〔Ｊ．Ｂｉ
ｏｌ．Ｃｈｅｍ．，２０３，１６７（１９５３）〕、ア
ルカリ水溶液で抽出する方法〔フランス特許第１，２４
５，８１８号（１９６１）〕、あるいは有機溶剤、界面
活性剤（ＳＤＳ）処理による蛋白変性除去による方法
（Ｊ．Ｃｈｅｍ．Ｓｏｃ．，１１２９〔１９４７），
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，１９０，１６５（１９５
１）〕などの報告があり、このようにして得た処理液の
酸沈殿、アルコール沈殿等によるＤＮＡの回収方法が知
られている。2. Description of the Related Art As a method for separating and collecting DNA from fish testis, so-called algae, extraction with saline [J. Bi
ol. Chem. , 203, 167 (1953)], a method of extracting with an aqueous alkali solution [French Patent Nos. 1,24
5,818 (1961)], or a method of removing protein denaturation by treatment with an organic solvent or a surfactant (SDS) (J. Chem. Soc., 1129 [1947),
J. Biol. Chem. , 190, 165 (195
1)] and the like, and a method of recovering DNA by acid precipitation, alcohol precipitation, etc. of the treatment liquid thus obtained is known.
【０００３】しかし、これらの方法で得られたＤＮＡは
分子量が高いことに基づき水溶液とする場合粘度が非常
に高くなり、取り扱い時に大量の水を加え粘度を低下さ
せる必要があり、高濃度にＤＮＡ溶液を作成することが
困難であり、ＤＮＡ含有の液状食品等への応用が妨げら
れる要因ともなっていた。However, the DNA obtained by these methods has a very high viscosity when made into an aqueous solution due to its high molecular weight, and it is necessary to add a large amount of water during handling to reduce the viscosity. It is difficult to prepare a solution, which is also a factor that hinders its application to liquid foods containing DNA.
【０００４】[0004]
【発明が解決しようとする課題】水溶性のＤＮＡすなわ
ちＤＮＡのアルカリ金属塩類は本出願前公知であるが、
これまでの製品は分子量が高すぎるため易溶性とは言え
なかった。本発明は水溶性が格段に向上し、液状食品な
どへの添加が容易となるＤＮＡアルカリ金属塩およびそ
の製造方法を提供することを目的とする。本発明は分子
量が低下し、高濃度溶液においても粘度の極めて低いＤ
ＮＡアルカリ金属塩およびその製造方法、すなわち水溶
性を格段に向上させたＤＮＡのアルカリ金属塩の製造方
法を提供することを目的とする。Although water-soluble DNA, that is, alkali metal salts of DNA, are known before the present application,
The products so far could not be said to be easily soluble because the molecular weight was too high. It is an object of the present invention to provide a DNA alkali metal salt having a markedly improved water solubility and being easily added to liquid foods and the like, and a method for producing the same. The present invention has a low molecular weight and has a very low viscosity even in a high-concentration solution.
It is an object of the present invention to provide an NA alkali metal salt and a method for producing the same, that is, a method for producing an alkali metal salt of DNA having significantly improved water solubility.
【０００５】[0005]
【課題を解決するための手段】ＤＮＡ含量が高いこと、
および入手しやすいことから、サケ、マス、ニシン、サ
バ、タラ等の魚類の白子（精巣）の生もしくは冷凍物は
従来よりＤＮＡの抽出材料とされている。これまでＤＮ
Ａの抽出に際し例えばアルカリはＤＮＡの分子を適度に
切断して抽出しやすくする作用があること、あるいは魚
類の白子の摩砕物にプロテアーゼを作用させて反応液を
得、該反応液からＤＮＡを回収する方法は知られている
が、急激な分子量の低下を引き起こさせる手段は知られ
ていない。本発明者らは、魚類の白子から抽出、分離さ
れる遊離型のＤＮＡが水中での加熱処理により急激な分
子量の低下が起きることを発見し、その発見を基に鋭意
研究し本発明を完成させたものである。[Means for Solving the Problems] High DNA content,
Since it is easily available, the raw or frozen product of the alga (testis) of fish such as salmon, trout, herring, mackerel, and cod has been conventionally used as a material for extracting DNA. So far DN
In the extraction of A, for example, an alkali has an action of appropriately cutting DNA molecules to facilitate extraction, or a protease is allowed to act on a ground product of fish shirako to obtain a reaction solution, and the DNA is recovered from the reaction solution. Although a method for doing so is known, a means for causing a rapid decrease in molecular weight is not known. The present inventors have found that the free DNA extracted and separated from the agar of fishes causes a rapid decrease in the molecular weight due to the heat treatment in water, and based on the finding, earnestly studied and completed the present invention. It was made.
【０００６】すなわち、本発明は魚類の白子から抽出、
分離される遊離型のＤＮＡに対し、水中で加熱処理を施
すことにより急激な分子量の低下を引き起こさせ、その
後アルカリで中和をすることにより、きわめて優れた水
溶性を示すＤＮＡのアルカリ金属塩を製造する方法であ
る。これにより溶液状の食品等への添加、高濃度ＤＮＡ
溶液等の製造に寄与するものである。上記アルカリ金属
塩としてナトリウム塩、カリウム塩などを用いる。[0006] That is, the present invention is to extract from the algae of fish,
The separated free DNA is subjected to a heat treatment in water to cause a rapid decrease in molecular weight, and then neutralized with an alkali to obtain an alkali metal salt of DNA exhibiting extremely excellent water solubility. It is a manufacturing method. As a result, high-concentration DNA can be added to foods in solution form.
It contributes to the production of solutions and the like. As the alkali metal salt, sodium salt, potassium salt or the like is used.
【０００７】本発明の処理の対象となる上記ＤＮＡ、す
なわち原料ＤＮＡは、魚類白子より得たＤＮＡの酸沈殿
物である。魚類の白子を均質にしたのちアルカリ水溶液
でｐＨ８以上とし、撹拌後固形分を分離し、濾液に酸を
加えて得ることのできる遊離型ＤＮＡである。The above-mentioned DNA to be treated by the present invention, that is, the raw material DNA, is an acid precipitate of DNA obtained from fish spores. It is a free DNA that can be obtained by homogenizing fish spores, adjusting the pH to 8 or more with an alkaline aqueous solution, separating the solid content after stirring, and adding an acid to the filtrate.
【０００８】本発明の魚類白子由来の水溶性に優れたＤ
ＮＡ塩類は以下のようにして製造される。原料ＤＮＡの
魚類白子より得たＤＮＡの酸沈殿物に水を加え、この混
合物を５０〜９０℃に加熱する。このような簡単な操作
により、ＤＮＡの主鎖がランダムに切断され分子量が低
下し高濃度溶液においても粘度のきわめて低いＤＮＡア
ルカリ金属塩を得ることができる。Water-soluble D derived from the fish milt of the present invention
NA salts are manufactured as follows. Water is added to the acid precipitate of DNA obtained from the fish spores of the raw material DNA, and this mixture is heated to 50 to 90 ° C. By such a simple operation, the DNA main chain is randomly cleaved to reduce the molecular weight, and a DNA alkali metal salt having an extremely low viscosity even in a high-concentration solution can be obtained.
【０００９】さらに具体的に説明すると、凍結した魚類
白子を原料として凍結白子は解凍後に肉ひき機等で均質
化し、食塩、アルカリを加える。液量は当初の白子均質
化液の３〜４倍程度とし、この際食塩濃度は２Ｍとなる
様に加え、またアルカリは水酸化ナトリウム、水酸化カ
リウム等を用いてｐＨを８以上、好ましくは９〜１１に
調整し、８０〜１００℃にて３０分〜２時間撹拌後遠心
分離により不要残さを除去し、得られた上清に塩酸、硫
酸等の鉱酸を添加してｐＨ２以下として遊離型のＤＮＡ
沈殿を得る。More specifically, using frozen fish sardine as a raw material, the frozen sardine is homogenized with a meat grinder after thawing, and salt and alkali are added. The amount of the solution is about 3 to 4 times that of the initial homogenizing solution of white sardine. At this time, the salt concentration is adjusted to 2M, and the alkali is adjusted to pH 8 or more, preferably using sodium hydroxide or potassium hydroxide. After adjusting to 9-11 and stirring at 80-100 ° C for 30 minutes-2 hours, unnecessary residue is removed by centrifugation, and mineral acids such as hydrochloric acid and sulfuric acid are added to the obtained supernatant to release it to pH 2 or less. Type of DNA
Get a precipitate.
【００１０】この得られた沈殿物を流水中で十分に水洗
いし、沈殿物の等倍から２倍量の水を加えた後、５０〜
９０℃にて３０分〜２時間加熱撹拌後、デカンテーショ
ンにより不溶物を除去後、得られた上清を水酸化ナトリ
ウム、水酸化カリウム等のアルカリで中和しｐＨ７の水
溶液とする。次いでこの水溶液を凍結乾燥もしくは噴霧
乾燥等の乾燥手段によりＤＮＡアルカリ金属塩の粉末と
して取得する。The obtained precipitate is thoroughly washed with running water, and equal to twice the amount of water of the precipitate is added, and then 50-
After heating and stirring at 90 ° C. for 30 minutes to 2 hours, the insoluble matter is removed by decantation, and the obtained supernatant is neutralized with an alkali such as sodium hydroxide or potassium hydroxide to obtain a pH 7 aqueous solution. Next, this aqueous solution is obtained as a powder of DNA alkali metal salt by a drying means such as freeze-drying or spray-drying.
【００１１】[0011]
【実施例】本発明を実施例によって説明する。本発明は
この実施例によって何ら限定されない。EXAMPLES The present invention will be described with reference to examples. The present invention is not limited by this embodiment.
【００１２】実施例１ サケの凍結白子１００ｇを解凍後肉ひき機により均質化
し、この均質化液を撹拌機付きのフラスコに移し、水３
１０ｍｌ食塩４８ｇを加え８５℃にて撹拌し２０％水酸
化ナトリウム溶液を添加してｐＨを１０．５としてＩ時
間撹拌し、次いで３０００Ｇ（５分）にて遠心分離して
固型分を除去し、得られた濾液に７％塩酸溶液を加えて
ｐＨを１．５とし、沈殿した遊離型のＤＮＡを集めた。
集めた遊離型のＤＮＡは流水でよく水洗いし、２倍重量
の水を加えた後８０℃で３０分間加熱し放冷後デカンテ
ーションにより不溶物を除去、上清に２０％水酸化ナト
リウム溶液を添加してｐＨ７に調整し、凍結乾燥してＤ
ＮＡナトリウム塩の粉末４．０ｇを得た。Ｓｃｈｉｍｉ
ｄｔ ｔｈａｎｎｈａｕｚｅｒ Ｓｃｈｎｅｉｄｅｒ法
（以下ＳＴＳ法と略す）により分析した結果、純度は９
２．９％であった。 （ＤＮＡ収率：対原料白子 ３．５％）Example 1 100 g of frozen salmon salmon roe was thawed and homogenized with a meat grinder.
48 ml of 10 ml of sodium chloride was added and stirred at 85 ° C., 20% sodium hydroxide solution was added to adjust pH to 10.5 and stirred for I hours, and then centrifuged at 3000 G (5 minutes) to remove solid components. Then, 7% hydrochloric acid solution was added to the obtained filtrate to adjust the pH to 1.5, and the precipitated free DNA was collected.
The collected free-form DNA was washed thoroughly with running water, added with twice the weight of water, heated at 80 ° C for 30 minutes, allowed to cool, and then decanted to remove insoluble matter. The supernatant was treated with 20% sodium hydroxide solution. Add to adjust pH to 7, freeze-dry and D
4.0 g of powder of NA sodium salt was obtained. Schimi
As a result of analysis by the dt ternhauser Schneider method (hereinafter abbreviated as STS method), the purity was 9
It was 2.9%. (DNA yield: 3.5% relative to raw material Shirako)
【００１３】実施例２ サケの凍結白子を実施例１と同様に処理して得た遊離型
ＤＮＡの沈殿物２０ｇに２倍重量の水を加えた後で７０
℃１時間加熱し放冷後デカンテーションして不溶物を除
去し、得られた上清１０％水酸化ナトリウム溶液でｐＨ
７に調整後凍結乾燥によりＤＮＡナトリウム塩の粉末
３．８ｇを得た。ＳＴＳ法で分析した結果、純度は９
２．６％であった。 （ＤＮＡ収量：対原料白子 ３．５％）Example 2 A frozen DNA of salmon was treated in the same manner as in Example 1 to obtain 20 g of a precipitate of free DNA, which was added with double weight of water, and then 70
After heating at ℃ for 1 hour and allowing to cool, decantation is performed to remove insoluble matter, and the resulting supernatant is adjusted to pH with 10% sodium hydroxide solution.
After adjusting to 7, freeze-drying gave 3.8 g of DNA sodium salt powder. As a result of analysis by the STS method, the purity is 9
2.6%. (DNA yield: 3.5% relative to raw material Shirako)
【００１４】比較例 サケの凍結白子を実施例１と同様に処理して得た遊離型
ＤＮＡの沈殿物２０ｇに２０倍量の水を加えた後、室温
にて１０％の水酸化ナトリウム溶液を加え撹拌溶解しｐ
Ｈ７に調整後凍結乾燥によりＤＮＡナトリウム塩の粉末
４．１ｇを得た。ＳＴＳ法で分析した結果、純度は８
８．０％であった。 （ＤＮＡ収量：対原料白子３．６％）Comparative Example 20 g of a free DNA precipitate obtained by treating frozen salmon of salmon in the same manner as in Example 1 was added with 20 times the amount of water, and then a 10% sodium hydroxide solution was added at room temperature. Add stirring and dissolve p
After adjusting to H7, freeze-drying gave 4.1 g of powder of DNA sodium salt. As a result of analysis by the STS method, the purity is 8
It was 8.0%. (DNA yield: 3.6% relative to raw material Shirako)
【００１５】実施例３ 実施例１、２および比較例で得たＤＮＡナトリウム塩に
ついて試験例１〜３でそれぞれ試験した。試験例１（溶液） 実施例１、２および比較例の各粉末を１ｇおよび１０ｇ
を採取し、それぞれ２０℃の水１００ｍｌを加え、５分
ごとに３０秒間振り混ぜる操作を行い、３０分後の状態
を、表１に示した。Example 3 The DNA sodium salts obtained in Examples 1 and 2 and Comparative Example were tested in Test Examples 1 to 3, respectively. Test Example 1 (Solution) 1 g and 10 g of each powder of Examples 1 and 2 and Comparative Example
Were collected, 100 ml of water at 20 ° C. was added, and the mixture was shaken and mixed every 5 minutes for 30 seconds, and the state after 30 minutes is shown in Table 1.
【００１６】[0016]
【表１】 [Table 1]
【００１７】実施例品は１０％濃度においても澄明な溶
液となったが、比較例品は１０％では粘度の高い塊状の
ダマとなり、完全に溶けきらなかった。The product of the example was a clear solution even at 10% concentration, but the product of the comparative example was not completely dissolved at 10% because it became a lumpy lump with high viscosity.
【００１８】試験例２（１％溶液の粘度測定） 実施例１、２および比較例の各粉末を１ｇ採取し、水を
加えてそれぞれ１００ｍｌとし、２０℃にてＢ型粘度計
によりＮｏ．１のローターを用いて回転数１００ｒｐ
ｍ、保持時間３０秒にて各溶液の粘度を測定した。結果
を表２に示した。 Test Example 2 (Measurement of Viscosity of 1% Solution) 1 g of each powder of Examples 1 and 2 and Comparative Example was sampled, and water was added to make 100 ml each. Rotation speed 100 rp using 1 rotor
The viscosity of each solution was measured at m and a holding time of 30 seconds. The results are shown in Table 2.
【００１９】[0019]
【表２】 [Table 2]
【００２０】試験例３（推定分子量の測定） 実施例１、２及び比較例で得られたＤＮＡナトリウム塩
の分子量を液体クロマトグラフ法で測定した。測定条件
は以下の通りである。 使用機器及び測定条件 ポンプ：日本分光製ＰＶ−９８０型 検出器：エルマ製示差屈折計ＥＲＣ−７５１２型 インテグレーター：ウォーターズ製８０５型データステ
ーション カラムオーブン：日本分光製８６５−ＣＯ型 カラム：ウォーターズ製ウルトラハイドロジェルリニア
ー（７．８×３００ｍｍ） 移動相：０．１Ｍ酢酸ナトリウム 流量：１．０ｍｌ／ｍｉｎ カラム温度：４５℃Test Example 3 (Measurement of Estimated Molecular Weight) The molecular weights of the DNA sodium salts obtained in Examples 1 and 2 and Comparative Example were measured by liquid chromatography. The measurement conditions are as follows. Equipment and measurement conditions Pump: JASCO PV-980 type Detector: Elma differential refractometer ERC-7512 type Integrator: Waters 805 data station Column oven: JASCO 86-CO column: Waters Ultra Hydro Gel linear (7.8 x 300 mm) Mobile phase: 0.1 M sodium acetate Flow rate: 1.0 ml / min Column temperature: 45 ° C
【００２１】分子量はショーデックス製プルラン標準品
を用いて、液体クロマトグラフで各分子量ごとの溶出時
間を測定し、分子量と溶出時間をプロットした較正曲線
を作成する。その後、試料溶液（実施例１，２品および
比較例品）を同じ液体クロマトグラフの測定条件で溶出
時間を測定し、この値を先の較正曲線上に当てはめその
推定分子量を算出した。結果を図１および表３に示し
た。For the molecular weight, a pullulan standard product manufactured by Shoredex was used to measure the elution time for each molecular weight by liquid chromatography, and a calibration curve was prepared by plotting the molecular weight and the elution time. Then, the elution time of the sample solutions (Examples 1 and 2 and Comparative Example) were measured under the same liquid chromatograph measurement conditions, and this value was applied to the above calibration curve to calculate the estimated molecular weight. The results are shown in FIG. 1 and Table 3.
【００２２】[0022]
【表３】 [Table 3]
【００２３】[0023]
【発明の効果】分子量が低下し、高濃度溶液においても
粘度の極めて低いＤＮＡアルカリ金属塩およびその製造
方法を提供することができる。水溶性が格段に向上し、
液状食品などへの添加が容易となるＤＮＡアルカリ金属
塩およびその製造方法を提供することができる。EFFECT OF THE INVENTION It is possible to provide a DNA alkali metal salt having a low molecular weight and an extremely low viscosity even in a high-concentration solution, and a method for producing the same. The water solubility is significantly improved,
It is possible to provide a DNA alkali metal salt that can be easily added to liquid foods and the like, and a method for producing the same.
【図１】プルラン標準品の較正曲線及び試料溶液（実施
例１，２品および比較例品）の測定結果を表す説明図で
ある。FIG. 1 is an explanatory diagram showing a calibration curve of a pullulan standard product and measurement results of sample solutions (Examples 1, 2 and comparative products).
───────────────────────────────────────────────────── フロントページの続き (72)発明者 秦 和彦 八王子市北野町559−６ 日本水産株式会 社中央研究所内 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kazuhiko Hata 559-6 Kitano-cho, Hachioji-shi Central Research Institute of Japan Fisheries Stock Company
Claims (7)
類。1. A salt of DNA derived from fish algae which has excellent water solubility.
である請求項１の水溶性に優れたＤＮＡ塩類。2. The DNA salt excellent in water solubility according to claim 1, which has a molecular weight reduced by heat treatment.
たは２の水溶性に優れたＤＮＡ塩類。3. The highly water-soluble DNA salt according to claim 1, wherein the salt is an alkali metal salt.
ＤＮＡを用い、これを水中で加熱処理することを特徴と
する魚類白子由来の水溶性に優れたＤＮＡ塩類の製造方
法。4. A method for producing a DNA salt having excellent water-solubility derived from fish spores, which comprises using free DNA derived from fish spores as a raw material DNA and heat-treating this in water.
水溶液とする請求項４の水溶性に優れたＤＮＡ塩類の製
造方法。5. The method for producing a DNA salt having excellent water solubility according to claim 4, wherein after the heat treatment, the solution is neutralized with an alkali to obtain an aqueous solution having a pH of 7.
２時間加熱撹拌することである請求項４または５の水溶
性に優れたＤＮＡ塩類の製造方法。6. The heat treatment is performed at 50 to 90 ° C. for 30 minutes or more.
The method for producing a DNA salt having excellent water solubility according to claim 4 or 5, which comprises heating and stirring for 2 hours.
ちアルカリ水溶液でｐＨ８以上とし、撹拌後固形分を分
離した濾液に酸を加えて得ることのできる遊離型のＤＮ
Ａである請求項４ないし６のいずれかの水溶性に優れた
ＤＮＡ塩類の製造方法。7. A free-form DNA which can be obtained by homogenizing fish spores, adjusting the pH of the raw DNA to 8 or higher with an aqueous alkali solution, and adding an acid to the filtrate from which solids have been separated after stirring.
7. The method for producing a DNA salt having excellent water solubility according to claim 4, which is A.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7216456A JPH0931093A (en) | 1995-07-22 | 1995-07-22 | Dna salt originating from soft roe of fish and having excellent water solubility and its production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7216456A JPH0931093A (en) | 1995-07-22 | 1995-07-22 | Dna salt originating from soft roe of fish and having excellent water solubility and its production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0931093A true JPH0931093A (en) | 1997-02-04 |
Family
ID=16688791
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7216456A Withdrawn JPH0931093A (en) | 1995-07-22 | 1995-07-22 | Dna salt originating from soft roe of fish and having excellent water solubility and its production |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0931093A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007291062A (en) * | 2006-03-31 | 2007-11-08 | Nissei Bio Kk | Compounding agent for base cosmetic, and base cosmetic |
| JP2009131222A (en) * | 2007-11-30 | 2009-06-18 | Maruha Nichiro Foods Inc | Nucleic acid material suitable for being mixing with drink, and method for producing the same |
| CN103819513A (en) * | 2014-03-05 | 2014-05-28 | 北京师范大学 | DNA (desoxyribonucleic acid) eluent and elution method |
| KR102190612B1 (en) | 2019-08-27 | 2020-12-14 | (주)모아캠 | Method of Producing Low Molecular Weight DNA derived from Plant Materials, Microbial Materials or Marine Materials |
-
1995
- 1995-07-22 JP JP7216456A patent/JPH0931093A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007291062A (en) * | 2006-03-31 | 2007-11-08 | Nissei Bio Kk | Compounding agent for base cosmetic, and base cosmetic |
| JP2009131222A (en) * | 2007-11-30 | 2009-06-18 | Maruha Nichiro Foods Inc | Nucleic acid material suitable for being mixing with drink, and method for producing the same |
| CN103819513A (en) * | 2014-03-05 | 2014-05-28 | 北京师范大学 | DNA (desoxyribonucleic acid) eluent and elution method |
| KR102190612B1 (en) | 2019-08-27 | 2020-12-14 | (주)모아캠 | Method of Producing Low Molecular Weight DNA derived from Plant Materials, Microbial Materials or Marine Materials |
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