CN115368486A - Ternary eutectic solvent and application thereof in extraction of procambarus clarkia shell chitin - Google Patents
Ternary eutectic solvent and application thereof in extraction of procambarus clarkia shell chitin Download PDFInfo
- Publication number
- CN115368486A CN115368486A CN202210854783.4A CN202210854783A CN115368486A CN 115368486 A CN115368486 A CN 115368486A CN 202210854783 A CN202210854783 A CN 202210854783A CN 115368486 A CN115368486 A CN 115368486A
- Authority
- CN
- China
- Prior art keywords
- eutectic solvent
- ternary eutectic
- extraction
- chitin
- procambarus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002904 solvent Substances 0.000 title claims abstract description 64
- 229920002101 Chitin Polymers 0.000 title claims abstract description 59
- 230000005496 eutectics Effects 0.000 title claims abstract description 59
- 238000000605 extraction Methods 0.000 title claims abstract description 36
- 241000238030 Procambarus clarkii Species 0.000 title claims abstract description 35
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims abstract description 36
- 239000000843 powder Substances 0.000 claims abstract description 26
- 238000001035 drying Methods 0.000 claims abstract description 22
- 238000010438 heat treatment Methods 0.000 claims abstract description 16
- 239000002244 precipitate Substances 0.000 claims abstract description 10
- 230000007935 neutral effect Effects 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 42
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 28
- 229910052739 hydrogen Inorganic materials 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 15
- 235000019743 Choline chloride Nutrition 0.000 claims description 15
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 15
- 229960003178 choline chloride Drugs 0.000 claims description 15
- 239000004310 lactic acid Substances 0.000 claims description 14
- 235000014655 lactic acid Nutrition 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 10
- 238000002791 soaking Methods 0.000 claims description 8
- 238000007873 sieving Methods 0.000 claims description 6
- 235000011054 acetic acid Nutrition 0.000 claims description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims 1
- 239000002253 acid Substances 0.000 abstract description 13
- 238000000034 method Methods 0.000 abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 238000010612 desalination reaction Methods 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000005580 one pot reaction Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 238000009210 therapy by ultrasound Methods 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000012295 chemical reaction liquid Substances 0.000 abstract 1
- 238000003760 magnetic stirring Methods 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 description 20
- 230000000694 effects Effects 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 238000005303 weighing Methods 0.000 description 8
- 238000005115 demineralization Methods 0.000 description 5
- 230000002328 demineralizing effect Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000007795 chemical reaction product Substances 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000011707 mineral Substances 0.000 description 4
- 229930014626 natural product Natural products 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001306243 Clarkia delicata Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000005588 protonation Effects 0.000 description 1
- 238000001878 scanning electron micrograph Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a ternary eutectic solvent and application thereof in extraction of procambarus clarkia shell chitin, and the ternary eutectic solvent specifically comprises the following components: collecting the processed shrimp shell of the procambarus clarkia, drying and crushing the shrimp shell after the steps of cleaning, decontaminating and deproteinizing, adding the procambarus clarkia shell powder into the prepared ternary eutectic solvent for heating, carrying out ultrasonic treatment, continuously carrying out magnetic stirring reaction for a period of time until the tissue structure of the shrimp shell powder is fully dissociated, then adding a back-extraction solvent into the reaction liquid at room temperature and centrifuging until the pH of the obtained precipitate is neutral, and drying the precipitate to obtain the chitin. The method has the advantages that the ternary eutectic solvent is efficiently extracted, the procambarus clarkii shell chitin with higher purity is obtained by a one-pot heating and ultrasonic microwave treatment method, strong acid desalination is not used, the production cost can be obviously reduced, chemical reagents in the purification process can be completely eradicated, the environmental pollution is reduced, and the extraction rate of the chitin can be greatly improved.
Description
Technical Field
The invention belongs to the technical field of natural product extraction, relates to a ternary eutectic solvent for natural product extraction, and particularly relates to a ternary eutectic solvent and application thereof in extraction of procambarus clarkia shell chitin.
Background
Procambarus clarkia (C. Clarkia)Procambarus clarkii) The method has strong growth adaptability and high propagation speed, and becomes an important economic category in the freshwater shrimp industry, in recent years, the procambarus clarkii industry develops rapidly, and the procambarus clarkii breeding yield in China in 2020 is counted to be 239.37 million tons, so that the industry drives the local industry development and social progress. At the same time, however, the non-edible parts of the procambarus clarkii are relatively high, and most of the shell is discarded or used at a low value.
The procambarus clarkia shell contains a large amount of chitin, is a natural high molecular compound, is insoluble in water, and has various characteristics of no toxicity, biodegradability, biocompatibility, chemical stability, thermal stability and the like, so that the biopolymers are attractive in various forms of industries and research fields of agriculture, medicines, foods, textiles and the like. However, due to its specific molecular structure, it is difficult to dissolve in most solvents, and in practice, it is only soluble in a few strong acid solutions, thus limiting the extraction efficiency.
In recent years, along with the intensive preparation and the intensive utilization of the procambarus clarkii chitin, chitin products are more widely utilized in the industries such as agriculture, food, biomedicine, chemical industry, cosmetics and the like. For a long time, strong acid calcium carbonate removal and alkali protein removal are adopted for industrial extraction of chitin. The method has large demand for strong acid and strong alkali, high cost for subsequent wastewater treatment, easy generation of serious environmental load and no contribution to the sustainable development of industry. In recent years, various novel green solvents are used for chitin extraction, wherein a eutectic solvent (DES) is applied to a certain extent, the eutectic solvent is a liquid compound which is formed by Hydrogen bonding of a Hydrogen Bond Acceptor (HBA) and a Hydrogen Bond Donor (HBD) and has a melting point lower than that of any component of the eutectic solvent, the liquid compound is in a liquid state at room temperature, and the eutectic solvent is widely applied in the chemical field due to the characteristics of good solubility, rich component sources, low price and the like. Compared with the common solvent extraction method, the eutectic solvent has obvious advantages in the extraction efficiency and the extraction effect in the field of natural product extraction, but more importantly, the extraction efficiency is improved, the obtained extract can keep the biological activity and is harmless to human beings, and the eutectic solvent becomes another development direction for developing and utilizing natural products in the fields of food, biology, catalysis and the like.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a ternary eutectic solvent, which is applied to extraction of procambarus clarkia shell chitin, so that the shrimp shell powder and the eutectic solvent are fully dissolved under ultrasonic and microwave treatment, impurities and calcium in the shrimp shell powder can be removed, and the chitin with high extraction rate and high purity is finally obtained.
The invention is realized by the following technical scheme:
a ternary eutectic solvent is prepared by mixing choline chloride, lactic acid and acetic acid, wherein the molar ratio of the choline chloride to the lactic acid to the acetic acid is (1) - (1). Wherein, choline chloride is a hydrogen bond acceptor, lactic acid and acetic acid are hydrogen bond donors, and the process of mixing preparation is as follows: heating at a certain temperature, and magnetically stirring for a period of time until uniform transparent liquid is generated, thereby obtaining the ternary eutectic solvent.
Further, when the choline chloride, the lactic acid and the acetic acid are mixed to prepare the ternary eutectic solvent, the temperature is 90-150 ℃, and the time is 8-24h.
The invention further improves the scheme as follows:
the ternary eutectic solvent is applied to extracting the chitin from the shell of the procambarus clarkia.
Further, the application of the ternary eutectic solvent in the extraction of the procambarus clarkia shell chitin comprises the following specific steps:
s1: collecting the shrimp shell of the procambarus clarkia, ultrasonically cleaning, removing foreign matters, soaking in the solution, heating and stirring for a period of time, then ultrasonically treating, washing with water, drying in a drying box, crushing by a high-speed crusher, and sieving to obtain shrimp shell powder for later use;
s2: adding the shrimp shell powder prepared in the step S1 into a ternary eutectic solvent, heating and stirring for a period of time under ultrasonic and microwave treatment until the tissue structure of the shrimp shell powder is fully dissociated and dissolved, adding back extraction solvent deionized water at room temperature, centrifuging to be neutral to obtain a precipitate, decoloring the precipitate with hydrogen peroxide, and drying to obtain the chitin.
Further, the solution used for soaking in the S1 is 90-100% ethanol solution, and the heating and stirring temperature is 25-40 ℃ and the time is 2-8 h.
Further, the drying temperature in the S1 is 90-100 ℃.
Further, the shrimp shell powder in the S1 passes through a filter sieve of 80 to 200 meshes.
Further, the mass ratio of the shrimp shell powder to the ternary eutectic solvent in S2 is 1 to 5-1.
Further, in S2, the ultrasonic power of ultrasonic treatment and microwave treatment is 50W/40kHz, the microwave heating temperature is 40-70 ℃, and the treatment time is 1-5 h.
Further, 10% H is used in S2 2 O 2 Decoloring at 50-90 deg.c.
The invention has the beneficial effects that:
the invention takes choline chloride as a hydrogen bond receptor and lactic acid and acetic acid as hydrogen bond donors to prepare a ternary eutectic solvent, and the solvent has a remarkable improvement effect on the extraction of the procambarus clarkii chitin.
Acid in the eutectic solvent can react with minerals in the shrimp shell of the procambarus clarkia to generate a demineralization effect, so that a fibril structure formed by combining chitin and protein is loosened to generate gaps, chlorine in a hydrogen bond receptor choline chloride has strong electronegativity and can form anion, cation and neutral ion hydrogen bonds, thereby being beneficial to formation of hydrated ions, leading ionization and protonation enhancement of the eutectic solvent and improving the demineralization effect. In addition, the eutectic solvent can form new hydrogen bonds with chitin and protein, and weaken the strong hydrogen bond effect in the chitin repeating unit, so that the valence bonds connecting chitin and other macromolecules can be destroyed, the original microstructures of chitin and protein are broken, the eutectic solvent is further favorable for swelling in the structure, and the rich active functional group structure in the protein can form new hydrogen bonds with hydrogen bond receptors, so that the hydrogen bonds in the molecules and among the molecules of the fibril structure are destroyed, the protein is separated from the structure, and the removal of the protein is accelerated. Intramolecular/intermolecular hydrogen bonds in the chitin are blocked by the eutectic solvent, and new hydrogen bonds are formed between chitin molecules and the eutectic solvent, so that the aim of separating the chitin is fulfilled.
The ternary eutectic solvent is used for extracting the procambarus clarkia shell chitin, and the mineral removal is mainly the effect of acid, but not all acid or strong acid can reach the result of the application, when single acid is used as a hydrogen bond donor, the demineralization effect is relatively poor when the acidity is weak, but when the acidity is strong, the demineralization effect is good, but the chitin is hydrolyzed into disaccharide by acid, so that the yield is obviously reduced, the strong acid can increase the surface modification of the chitin, and the esterification reaction between hydroxyl of the chitin and carboxyl of the acid in the hydrogen bond donor can be improved, so that the final extraction effect and quality are influenced. When lactic acid and acetic acid are used as hydrogen bond donors, hydrogen bonds with more quantity and richer structure are formed, and the acidic condition of maintaining buffer balance is more favorable for exerting the demineralization effect.
The method adopts the efficient extraction of the ternary eutectic solvent, obtains the high-purity procambarus clarkii shell chitin by a heating and ultrasonic microwave treatment one-pot method, does not use strong acid for desalination, not only can obviously reduce the production cost, but also can stop chemical reagents in the purification process, reduce the environmental pollution, and simultaneously can greatly improve the extraction rate of the chitin.
Drawings
Fig. 1 is SEM images of chitin (a), commercial chitin (b) and shrimp shell (c) extracted by ternary eutectic solvent.
As can be seen from fig. 1, the extracted chitin has significantly changed in morphology and surface compared to shrimp shells. Due to the existence of minerals and proteins, the surface of the shrimp shell is rough and has no pores. For chitin extracted by eutectic melting, the surface is smooth and porous due to the removal of minerals and proteins.
Detailed Description
Example 1
Preparing shrimp shell powder: collecting the shrimp shell of procambarus clarkia, ultrasonically cleaning, removing foreign matters, soaking in 90-100% ethanol solution at 25-40 ℃, heating and stirring for 2-8 h, then ultrasonically treating, washing with water, drying in a drying oven at 90-100 ℃, crushing by a high-speed crusher, and sieving by a 120-mesh filter screen to obtain the shrimp shell powder for later use.
Preparing a ternary eutectic solvent: the choline chloride, the acetic acid and the lactic acid are weighed according to a molar ratio of 1.
Extracting chitin: weighing shrimp shell powder according to the mass ratio of 1. Adding distilled water into the reaction product cooled to room temperature, cooling, centrifuging on a high-speed centrifuge, repeatedly washing with water to be nearly neutral, and drying the precipitate to obtain white sheet-shaped matter, namely the chitin. The extraction rate of the chitin is 28.06%, the ash content is 0.25%, and the protein content is 3.51% by calculation after weighing.
Example 2
Preparing shrimp shell powder: collecting the shrimp shell of the procambarus clarkia, ultrasonically cleaning, removing foreign matters, soaking in 90-100% ethanol solution, heating and stirring at 30-35 ℃ for 2-8 h, then ultrasonically treating, washing with water, drying in a drying oven at 90-100 ℃, crushing by a high-speed crusher, and sieving by a 200-mesh filter screen to obtain the shrimp shell powder for later use.
Preparing a ternary eutectic solvent: the choline chloride, the acetic acid and the lactic acid are weighed according to a molar ratio of 1.
Extracting chitin: weighing shrimp shell powder according to the mass ratio of 1. Adding distilled water into the reaction product cooled to room temperature, cooling, centrifuging in a high-speed centrifuge, repeatedly washing with water to be neutral, and drying the precipitate to obtain white sheet-like substance to obtain chitin. The extraction rate of chitin after weighing is 31.26%, the ash content is 0.30%, and the protein content is 3.40%.
Example 3
Preparing shrimp shell powder: collecting the shrimp shell of procambarus clarkia, ultrasonically cleaning, removing foreign matters, soaking in 90-100% ethanol solution at 25-30 ℃, heating and stirring for 2-8 h, then ultrasonically treating, washing with water, drying in a drying oven at 90-100 ℃, crushing by a high-speed crusher, and sieving by a 120-mesh filter screen to obtain the shrimp shell powder for later use.
Preparing a ternary eutectic solvent: and (2) weighing choline chloride, acetic acid and lactic acid according to a molar ratio of 1.
Extracting chitin: weighing shrimp shell powder according to the mass ratio of 1. Adding distilled water into the reaction product cooled to room temperature, cooling, centrifuging in a high-speed centrifuge, repeatedly washing with water to be neutral, and drying the precipitate to obtain white sheet-like substance to obtain chitin. The extraction rate of the chitin is 30.46 percent, the ash content is 0.28 percent and the protein content is 3.70 percent after the chitin is weighed.
Example 4
Preparing shrimp shell powder: collecting the shrimp shell of the procambarus clarkia, ultrasonically cleaning, removing foreign matters, soaking in 90-100% ethanol solution, heating and stirring at 30-40 ℃ for 2-8 h, then ultrasonically treating, washing with water, drying in a drying oven at 90-100 ℃, crushing by a high-speed crusher, and sieving by a 200-mesh filter screen to obtain the shrimp shell powder for later use.
Preparing a ternary eutectic solvent: and (2) weighing choline chloride, acetic acid and lactic acid according to the molar ratio of 1.
Extracting chitin: weighing shrimp shell powder according to the mass ratio of 1. Adding distilled water into the reaction product cooled to room temperature, cooling, centrifuging on a high-speed centrifuge, repeatedly washing with water to be nearly neutral, and drying the precipitate to obtain white sheet-shaped matter, namely the chitin. The extraction rate of the chitin is 29.86 percent, the ash content is 0.31 percent and the protein content is 3.45 percent after the chitin is weighed.
Comparative example 1
The binary eutectic solvent prepared from choline chloride and lactic acid with the molar ratio of 1.
Comparative example 2
The binary eutectic solvent prepared from choline chloride and acetic acid with the molar ratio of 1.
Claims (10)
1. The ternary eutectic solvent is characterized by being prepared by mixing choline chloride, lactic acid and acetic acid, wherein the molar ratio of the choline chloride to the lactic acid to the acetic acid is 1.
2. A ternary eutectic solvent according to claim 1, characterized in that: when choline chloride, lactic acid and acetic acid are mixed to prepare the ternary eutectic solvent, the temperature is 90-150 ℃, and the time is 8-24h.
3. A ternary eutectic solvent as claimed in any one of claims 1 or 2 applied to procambarus clarkia shell chitin extraction.
4. The application of the ternary eutectic solvent in extraction of procambarus clarkii shell chitin according to claim 3, is characterized by comprising the following specific steps:
s1: collecting the shrimp shell of the procambarus clarkia, ultrasonically cleaning, removing foreign matters, soaking in the solution, heating and stirring for a period of time, then ultrasonically treating, washing with water, drying in a drying box, crushing by a high-speed crusher, and sieving to obtain shrimp shell powder for later use;
s2: adding the shrimp shell powder prepared in the step S1 into a ternary eutectic solvent, heating and stirring for a period of time under ultrasonic and microwave treatment until the tissue structure of the shrimp shell powder is fully dissociated and dissolved, adding back extraction solvent deionized water at room temperature, centrifuging to be neutral to obtain a precipitate, decoloring the precipitate with hydrogen peroxide, and drying to obtain the chitin.
5. The use of the ternary eutectic solvent in extraction of procambarus clarkii shell chitin according to claim 4, wherein the ternary eutectic solvent comprises: the solution used for soaking in the S1 is 90-100% ethanol solution, and the heating and stirring temperature is 25-40 ℃ and the time is 2-8 h.
6. The use of the ternary eutectic solvent in extraction of procambarus clarkii shell chitin according to claim 4, wherein the ternary eutectic solvent comprises: the drying temperature in the S1 is 90-100 ℃.
7. The use of the ternary eutectic solvent in extraction of procambarus clarkii shell chitin according to claim 4, wherein the ternary eutectic solvent is selected from the group consisting of: s1, screening the medium shrimp shell powder by a filter screen of 80-200 meshes.
8. The use of the ternary eutectic solvent in extraction of procambarus clarkii shell chitin according to claim 4, wherein the ternary eutectic solvent comprises: in S2, the mass ratio of the shrimp shell powder to the ternary eutectic solvent is 1 to 5-1.
9. The use of the ternary eutectic solvent in extraction of procambarus clarkii shell chitin according to claim 4, wherein the ternary eutectic solvent comprises: and in S2, the ultrasonic power of ultrasonic and microwave treatment is 50W/40kHz, the microwave heating temperature is 40-70 ℃, and the treatment time is 1-5 h.
10. The use of the ternary eutectic solvent in extraction of procambarus clarkii shell chitin according to claim 4, wherein the ternary eutectic solvent is selected from the group consisting of: s2 is 10% H 2 O 2 Decoloring at 50-90 deg.c.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210854783.4A CN115368486B (en) | 2022-07-20 | 2022-07-20 | Ternary eutectic solvent and application thereof in procambarus clarkia shell chitin extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210854783.4A CN115368486B (en) | 2022-07-20 | 2022-07-20 | Ternary eutectic solvent and application thereof in procambarus clarkia shell chitin extraction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115368486A true CN115368486A (en) | 2022-11-22 |
| CN115368486B CN115368486B (en) | 2023-12-22 |
Family
ID=84061276
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210854783.4A Active CN115368486B (en) | 2022-07-20 | 2022-07-20 | Ternary eutectic solvent and application thereof in procambarus clarkia shell chitin extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115368486B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115594778A (en) * | 2022-11-23 | 2023-01-13 | 中国海洋大学（Cn） | Method for extracting chitin by using acidic eutectic solvent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112726253A (en) * | 2021-01-21 | 2021-04-30 | 天津科技大学 | Method for efficiently separating plant fibers by acidic eutectic solvent/metal salt multi-component system |
| CN113046400A (en) * | 2021-03-24 | 2021-06-29 | 中南大学 | Method for ultra-fast pretreatment of lignocellulose in biomass |
| CN114044835A (en) * | 2021-12-07 | 2022-02-15 | 南京林业大学 | Method for extracting chitin from crayfish shells by microwave-assisted eutectic solvent |
-
2022
- 2022-07-20 CN CN202210854783.4A patent/CN115368486B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112726253A (en) * | 2021-01-21 | 2021-04-30 | 天津科技大学 | Method for efficiently separating plant fibers by acidic eutectic solvent/metal salt multi-component system |
| CN113046400A (en) * | 2021-03-24 | 2021-06-29 | 中南大学 | Method for ultra-fast pretreatment of lignocellulose in biomass |
| CN114044835A (en) * | 2021-12-07 | 2022-02-15 | 南京林业大学 | Method for extracting chitin from crayfish shells by microwave-assisted eutectic solvent |
Non-Patent Citations (5)
| Title |
|---|
| AMERI, MARYAM AL.: "Deep Eutectic Solvent Pretreatment for Enhancing Biochemical Conversion of Switchgrass", pages 18 - 19 * |
| MOHAMMAD KHAJAVIAN ET AL: "Chitin and derivative chitosan-based structures — Preparation strategies aided by deep eutectic solvents: A review", CARBOHYDRATE POLYMERS, vol. 275, pages 1 - 10 * |
| MOHAMMAD KHORSANDI ET AL: "Measurement and correlation of coumarin solubility in aqueous solution of acidic deep eutectic solvents based on choline chloride", vol. 524, pages 1 - 11 * |
| VERONIKA MAHOVA ET AL: "Delighnification of unbleached pulp by ternary deep eutectic solvents", vol. 10, no. 1, pages 666 - 676 * |
| ZIYAN LI ET AL: "Recent advances in extraction and processing of chitin using deep eutectic solvents", CHEMICAL ENGINEERING JOURNAL, vol. 446, pages 1 - 10 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115594778A (en) * | 2022-11-23 | 2023-01-13 | 中国海洋大学（Cn） | Method for extracting chitin by using acidic eutectic solvent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115368486B (en) | 2023-12-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102174627B (en) | Method for preparing rapeseed bioactive peptide | |
| JP5972165B2 (en) | Post-extraction method for low-order acylated gellan gum with high solubility | |
| CN104558238B (en) | Process for extracting sodium alginate | |
| CN102115690A (en) | Method for comprehensively utilizing rice bran | |
| CN110699411B (en) | Preparation method of eggshell membrane polypeptide | |
| JP2015192675A (en) | Rice protein composition and production method of the same | |
| JP3278629B2 (en) | Method for separating and purifying chondroitin sulfate | |
| CN115368486A (en) | Ternary eutectic solvent and application thereof in extraction of procambarus clarkia shell chitin | |
| WO2010013174A2 (en) | Process for the co-production of chitin, its derivatives and polymers containing glucose, mannose and/or galactose, by the fermentation of the yeast pichia pastoris | |
| FI127267B (en) | Process for separating proteins from biomass materials | |
| CN103113488B (en) | The extracting method of the low acyl transparent type gelling gum of a kind of easy dispersion and dissolving | |
| CN102732592A (en) | Method for preparing freshwater fish bone gelatin by enzyme process | |
| CN101787082A (en) | Method for removing thalli in process of producing curdlan | |
| CN114957386A (en) | Method for producing collagen tripeptide from fish scales | |
| CN102702384A (en) | Method for removing proteins in chitin material | |
| Mariod | Extraction, purification, and modification of natural polymers | |
| JP2870871B2 (en) | A method for treating crustacean shells using enzymes | |
| CN103421871A (en) | Preparation method of tuna bone collagen peptide | |
| CN112175098A (en) | Method for efficiently preparing polysaccharide from sparassis crispa stipe | |
| CN108912246B (en) | Method for extracting chitin by comprehensively treating and utilizing shrimp and crab shells | |
| CN108101980B (en) | Preparation method of high-purity phycocyanin | |
| CN106591406B (en) | Method for preparing bone gelatin by using ultrasonic-assisted enzyme method | |
| CN106381744A (en) | Method for separating nano cellulose from reeds | |
| CN114044835A (en) | Method for extracting chitin from crayfish shells by microwave-assisted eutectic solvent | |
| JP2007097466A (en) | Method for producing n-acetyl-d-glucosamine by enzymatic hydrolysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |