JPH08172901A - Method for carrying out artificial culture of cordyceps sinensis sacc. and artificial culturing substrate - Google Patents
Method for carrying out artificial culture of cordyceps sinensis sacc. and artificial culturing substrateInfo
- Publication number
- JPH08172901A JPH08172901A JP6340819A JP34081994A JPH08172901A JP H08172901 A JPH08172901 A JP H08172901A JP 6340819 A JP6340819 A JP 6340819A JP 34081994 A JP34081994 A JP 34081994A JP H08172901 A JPH08172901 A JP H08172901A
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- Japan
- Prior art keywords
- cordyceps sinensis
- cordyceps
- culture substrate
- artificial
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
- Mushroom Cultivation (AREA)
Abstract
Description
【０００１】[0001]
【０００２】本発明は、冬虫夏草の大量生産を企図した
冬虫夏草の栽培方法に関し、具体的には、乾燥蛹を培養
基材とする冬虫夏草の人工培養方法及び人工培養基材に
係るものである。[0002] The present invention relates to a method for cultivating Cordyceps for mass production of Cordyceps, and more particularly to a method for artificially cultivating Cordyceps using dried pupae as a culture substrate and an artificial culture substrate.
【０００３】[0003]
【従来の技術】冬虫夏草は、土中の昆虫や蜘蛛など（以
下、単に「虫」という。）に寄生した菌類が冬には不活
性で宿主の虫のみが活動しているが、初夏には前記寄生
の菌類が発芽して棒状その他の子実体に生育することに
鑑みて命名された語句である。そして、この子実体は虫
の中に天然でのみ生育することから、これを広い範囲の
原野で探し求めて採集し、調理又は医薬の素材原料とし
ている。それだけに前記子実体の採集は困難であり、中
国では前記菌類及び子実体は貴重な漢方薬品、健康食品
として使用されている。2. Description of the Related Art Cordyceps are inactive in the winter when fungi parasitizing insects and spiders in the soil (hereinafter simply referred to as "insects") are active in the winter, but only host insects are active. It is a term named in view of the fact that the parasitic fungus germinates and grows into rod-shaped or other fruiting bodies. Since this fruiting body grows naturally only in insects, it is searched and collected in a wide range of fields and used as a raw material for cooking or medicine. As a result, it is difficult to collect the fruiting bodies, and in China, the fungi and fruiting bodies are used as valuable Chinese herbs and health foods.
【０００４】[0004]
【発明が解決しようとする課題】近年、前記菌類及び子
実体の健康食品としての効果や、制癌作用等の薬効が解
明されつつあり、健康食品及び漢方薬品としての需要が
高まっている。しかし、その菌類及び子実体（以下、
「冬虫夏草」という）は天然にのみ生育されたものを採
集収穫するだけであるから、その採集収穫の労力負担は
大きく、しかも、前記健康食品及び漢方薬品としての需
要を十分に満たすことができない。そのため、大多数の
国民を占める庶民に安価に、かつ、十分に前記健康食品
及び漢方薬品を提供できず、国民全体の健康の維持と増
強を図るという観点から問題がある。そこで、本発明者
は、このような問題を解決すべく冬虫夏草を安価に、か
つ、大量に生産できる栽培方法はないものかと模索し
た。In recent years, the effects of the above-mentioned fungi and fruiting bodies as health foods and the medicinal effects such as carcinostatic action have been elucidated, and the demands as health foods and herbal medicines are increasing. However, the fungus and fruiting body (hereinafter,
Since "caterpillar" is only collected and harvested from the ones grown only in nature, the labor burden of the collection and harvest is large, and the demand for the health food and the herbal medicine cannot be sufficiently satisfied. Therefore, there is a problem from the viewpoint of maintaining and enhancing the health of the entire population because it is not possible to inexpensively and adequately provide the health food and the herbal medicine to the common people who make up the majority of the population. Therefore, the present inventors sought to find a method for cultivating Cordyceps for a large amount at a low cost in order to solve such a problem.
【０００５】その結果、チョウ、ガなどの鱗し目の昆虫
は我々の身の廻りに多く、特に、この鱗し目に属し、人
間の衣生活に密接な関係のある蚕は、長年人工的に大量
に孵化生育がなされた昆虫であり、これらが繭を生産す
るときには必ず蛹も同時的に生産されるから、人工的に
大量の繭を生産することは容易であり、これらの蛹を冬
虫夏草菌の培養基材として利用できれば、前記冬虫夏草
の生産の大量化・企業化が容易に行われる筈だという確
信を得た。本発明は、このような確信の基に鋭意研究と
実験を行ったところ、その蛹に元菌となる冬虫夏草菌を
接種した場合には、鋸屑等の他の培養基材に比べ、これ
の感染と繁殖が良好であるという知見を得、この新知見
に基いて完成されたもので、冬虫夏草の人工的生産に適
する冬虫夏草の人工培養方法及び人工培養基材を提供す
るものである。As a result, there are many lepidopteran insects such as butterflies and moths around us. Especially, silkworms belonging to this lepidopteran, which are closely related to human clothing life, have been artificially produced for many years. It is easy to artificially produce a large amount of cocoons because it is an insect that has been hatched and grown in large numbers, and pupae are always produced at the same time when they produce cocoons. We convinced that mass production and commercialization of the Cordyceps sinensis would be facilitated if it could be used as a culture substrate for bacteria. The present invention was conducted through diligent research and experimentation based on such conviction, and when the pupa was inoculated with Cordyceps sinensis, which is the original fungus, compared to other culture substrates such as sawdust, the infection of this The present invention has been completed on the basis of this new finding, and provides a method for artificially cultivating Cordyceps sinensis and an artificial culture substrate which are suitable for artificially producing Cordyceps sinensis.
【０００６】[0006]
【課題を解決するための手段】上記目的を達成するため
に、請求項１の発明に係る冬虫夏草の人工培養方法は、
蛹系の培養基材を殺菌した後、該培養基材に冬虫夏草の
元菌を接種し、暗条件の所定温度で冬虫夏草菌を培養さ
せることを特徴とし、請求項２の発明に係る冬虫夏草の
人工培養方法は、冬虫夏草の元菌が、原野で採集した天
然の冬虫夏草菌叉は人工培養で生成した冬虫夏草菌であ
ることを特徴とし、請求項３の発明に係る冬虫夏草の人
工培養基材は、完全変態を行う昆虫類の蛹を乾燥処理し
冬虫夏草菌の培地に調整したことを特徴とし、請求項４
の発明に係る冬虫夏草の人工培養基材は、乾燥蚕蛹を冬
虫夏草菌の培地に調整して得られることを特徴とし、請
求項５の発明に係る冬虫夏草の人工培養基材は、乾燥蚕
蛹を粉砕して得られる蚕蛹粉末と、魚粉及び／叉は骨粉
とを配合して冬虫夏草菌の培地に調整したことを特徴と
する。In order to achieve the above object, the method for artificially cultivating Cordyceps sinensis according to the invention of claim 1 comprises:
After sterilizing the pupa-based culture substrate, the culture substrate is inoculated with a caterpillar fungus, and the caterpillar fungus is cultivated at a predetermined temperature under dark conditions. The culturing method is characterized in that the original fungus of Cordyceps is a natural Cordyceps fungus or a Cordyceps fungus produced by artificial culture collected in the field, and the artificial culture substrate of Cordyceps sinensis according to the invention of claim 3 is completely The pupa of a metamorphosing insect is subjected to a drying treatment to prepare a medium for Cordyceps sinensis.
The artificial culture substrate of Cordyceps sinensis according to the invention is characterized in that it is obtained by adjusting dried silkworm pupae in a medium of Cordyceps sinensis, and the artificial culture substrate of Cordyceps sinensis according to the invention of claim 5 is obtained by crushing dried silkworm pupae. It is characterized in that the silkworm pupa powder obtained as described above is mixed with fish meal and / or bone meal to prepare a medium for Cordyceps sinensis.
【０００７】[0007]
【作用】請求項１の発明によれば、冬虫夏草の元菌が蛹
系の培養基材に感染して無限に増加繁殖し、その培養基
材の全表面積を覆って菌糸体を生成し、従って、冬虫夏
草が人工的に大量に栽培される。According to the first aspect of the present invention, the original fungus of the Cordyceps sinensis infects the pupal-type culture substrate to multiply and grow indefinitely, and covers the entire surface area of the culture substrate to form mycelia, and , Cordyceps are artificially cultivated in large quantities.
【０００８】請求項２の発明によれば、天然の数少ない
冬虫夏草菌が元菌となって培養基材で冬虫夏草菌が人工
的に増加繁殖する培養が行われ、さらに、その人工培養
された冬虫夏草菌は、そのまま同じ培養基材で菌糸体か
ら子実体へと生育し冬虫夏草が栽培されるし、又、元菌
として別な培養基材に接種されれば、そこでも冬虫夏草
菌が増加繁殖するといった具合に冬虫夏草が人工的に次
々に拡大して栽培される。According to the second aspect of the present invention, the rare natural caterpillar fungus is used as a source of culture, and the culture substrate is artificially increased and propagated in the culture substrate. Further, the artificially cultured caterpillar fungus is used. Is that the mycelium grows from the mycelium to fruit bodies on the same culture substrate as it is, and the Cordyceps sinensis is cultivated. The caterpillars are artificially expanded and cultivated one after another.
【０００９】請求項３の発明によれば、冬虫夏草菌を人
工培養基材たる乾燥蛹に接種すると、その冬虫夏草菌は
その乾燥蛹に良く感染し、該乾燥蛹をあたかも本来の天
然の宿主と同等の培地として生育繁殖し、大量に人工培
養される。According to the third aspect of the present invention, when Cordyceps sinensis is inoculated into dried pupae, which is an artificial culture substrate, the Cordyceps bacilli often infect the dried pupae, and the dried pupae are equivalent to the original natural host. It grows and propagates as a medium of and is artificially cultured in large quantities.
【００１０】請求項４の発明によれば、養蚕の排出物た
る乾燥蚕蛹が特別人工処理を施さずそのまま冬虫夏草の
元菌の培養基材に利用され、大規模で企業ペースで冬虫
夏草菌を人工培養する培地となる。According to the invention of claim 4, dried silkworm pupae, which are sericultural effluents, are used as they are as a culture substrate for the original fungus of Cordyceps sinensis without any special artificial treatment, and large-scale artificial culture of Cordyceps sinensis is conducted at a company pace. It becomes the culture medium.
【００１１】請求項５の発明によれば、人工培養基材自
体がＮ、Ｐ、Ｋ等の冬虫夏草菌の生育の必要な養分を十
分に供給し、その冬虫夏草菌は、前記養分を宋養として
繁殖を旺盛にし、十二分に人工培養されると共に、さら
に菌糸体にまで生成される。According to the invention of claim 5, the artificial culture substrate itself sufficiently supplies N, P, K and other nutrients necessary for the growth of the Cordyceps fungus, and the Cordyceps fungus uses the nutrients as a nutrient. With vigorous breeding, it is more than artificially cultivated, and even mycelium is produced.
【００１２】[0012]
【実施例】以下、本発明に係る冬虫夏草の人工培養方法
及び人工培養基材の実施例を、実験例及び栽培例を参照
しながら詳細に説明する。先ず、蛹系の培養基材を殺菌
する。この工程は、元菌となる後記冬虫夏草菌を培養基
材に接種した後、前記元菌を雑菌から防衛しつつ順調に
繁殖生育させるためのものである。培養基材を蛹系に限
定するのは、鋸屑等の農林産副産物や穀類等の農産物な
ど非蛹系の培地には殆ど後記冬虫夏草菌の感染がみられ
ないからである。蛹系の培養基材としては、酵母エキ
ス、ポリペプトン、ブドウ糖、寒天からなる完全合成培
地（酵母エキス寒天培地）、乾燥蛹、ブドウ糖、寒天か
らなる半合成培地（蚕蛹エキス培地）、乾燥蛹のみから
なる乾燥蛹培地、蚕蛹粉末と魚粉及び／叉は骨粉の配合
からなる配合培地等が採用されるが、生産コスト（生産
量、調整手間）から乾燥蛹の培地が好適である。EXAMPLES Examples of the method for artificially cultivating Cordyceps sinensis and the artificial culture substrate according to the present invention will be described in detail below with reference to experimental examples and cultivation examples. First, the pupal culture substrate is sterilized. This step is to inoculate the culture substrate with the later-mentioned Cordyceps sinensis, which is the original bacterium, and then to smoothly propagate and grow the original bacterium while protecting it from various bacteria. The reason for limiting the culture substrate to the pupa system is that almost no infection by Cordyceps sinensis is found in non-pupal culture media such as agricultural and forestry by-products such as sawdust and agricultural products such as cereals. As a pupal culture substrate, a complete synthetic medium consisting of yeast extract, polypeptone, glucose and agar (yeast extract agar medium), dried pupa, semi-synthetic medium consisting of glucose and agar (silkworm pupa extract medium), and dried pupae only A dry pupal medium, a mixed medium comprising a mixture of silkworm pupa powder and fish meal and / or bone meal, and the like are used, but a dried pupa medium is preferable from the viewpoint of production cost (production amount, adjustment labor).
【００１３】乾燥蛹の培地は、完全変態を行う昆虫類の
蛹を乾燥処理し、冬虫夏草菌の培地に調整したものであ
るが、乾燥蛹はカブト虫、せみ、蚕等の蛹をいうが、大
量生産性や培地調整労力の省力等の観点から乾燥蚕蛹が
好ましい。即ち、培養基材としての乾燥蛹は多くは魚類
のえさとして市販されている乾燥蚕蛹が使用される。大
量にかつコスト安に購入できるからであり、この点が本
発明の重要なポイントの１つである。しかし、現在大量
に使用されていなくても、カブト虫の蛹等が将来大量に
使用される可能性があれば、カブト虫の蛹その他の蛹を
も使用することができる。また、乾燥蛹は蚕蛹粉末と魚
粉、骨粉の配合物に代えると、冬虫夏草菌の繁殖を旺盛
にすることができる。その配合割合は７０〜８５
（％）：５〜１５（％）：５〜１５（％）で良好な結果
を得た。The medium of dried pupae is prepared by subjecting the pupae of insects that undergo complete metamorphosis to a drying treatment to prepare a medium of Cordyceps sinensis, and dried pupae refers to pupae such as beetles, cicadas, and silkworms. Dry silkworm pupae are preferable from the viewpoints of productivity and labor saving of culture medium adjustment labor. That is, as dried pupae as a culture substrate, dried pupae commercially available as food for fish are mostly used. This is one of the important points of the present invention because it can be purchased in large quantities and at low cost. However, if there is a possibility that a large amount of beetle pupae and the like will be used in the future even if they are not used in large amounts at present, pupae of beetle insects and other pupae can also be used. In addition, if dried pupae are replaced with a mixture of silkworm pupa powder, fish meal, and bone meal, the reproduction of Cordyceps sinensis can be enhanced. The mixing ratio is 70-85
Good results were obtained at (%): 5 to 15 (%): 5 to 15 (%).
【００１４】殺菌は、培養基材１００ｇ当たり水道水１
００ｍｌを加え、１１５℃乃至１２５℃好しくは１２０
℃の温度条件下で１５分乃至２５分好しくは２０分間を
蒸気に通す常法で行う滅菌である。滅菌とするのは、培
養基材の有効成分の消失防止、作業時間短縮等を考慮し
た商業殺菌で足りるからである。従って、完全殺菌を要
件としない。For sterilization, 1 tap water per 100 g of culture substrate is used.
Add 00 ml, 115 ° C to 125 ° C, preferably 120 ° C
Sterilization is carried out by a conventional method of passing steam for 15 minutes to 25 minutes, preferably 20 minutes under a temperature condition of ° C. The reason for sterilization is that commercial sterilization is sufficient in consideration of prevention of loss of the active ingredient of the culture substrate and reduction of working time. Therefore, complete sterilization is not a requirement.
【００１５】次に、前工程で殺菌を終えた培養基材に冬
虫夏草菌を接種する。冬虫夏草菌は、土中の昆虫・蜘蛛
などに寄生する菌類であって、真正子嚢菌または不完全
菌類のクモタケ、ハエに寄生するハエヤドリタケ等がこ
れに属し一次的元菌をなす。従って、この一次的元菌と
なる冬虫夏草菌は野性に探し求めて採集する冬虫夏草か
ら得ることになり、市販されていない菌である点が本発
明の重要なポイントの１つとなる。原野で多く採集され
た冬虫夏草としては、カメムシタケ（Ｃｏｒｄｙｃｅｐ
ｓ ｎｕｔａｎｓ Ｐａｔ）、ハナサナギタケ（Ｉｓａ
ｒｉａ ｊａｐｏｎｉｋａ Ｙａｓｕｄａ）、エダウチ
カメムシタケ（Ｈｉｒｓｕｔｅｌｌａ ｎｕｔａｎｓ
Ｋｏｂａｙａｓｉ）、ハスノミクモタケ（Ｃ．ｎｅｔｕ
ｍｂｏｉｄｅｓ Ｙ．Ｋｏｂａｙａｓｉ ｅｔ Ｄ．Ｓ
ｈｉｍｉｚｕ）等があげられる。Then, the Cordyceps sinensis is inoculated on the culture substrate that has been sterilized in the previous step. Cordyceps fungi are fungi that parasitize insects, spiders, and the like in the soil, and eoscomycetes or imperfect fungi such as spider moss and fly moss that are parasitic on flies belong to this species and are the primary bacteria. Therefore, the Cordyceps sinensis that is a primary fungus is obtained from Cordyceps sinensis that is searched for in the wild and collected, and one of the important points of the present invention is that it is not a commercially available fungus. Cordyceps (Cordycep) are the most often collected Cordyceps in the wilderness.
s nutans Pat), Pleurotus ostreatus (Isa)
ria japonika Yasuda), Hirutella nutans
Kobayashi), C. netu
mboides Y. Kobayasi et D. S
Himitsu) and the like.
【００１６】次に、前工程で接種した冬虫夏草菌が培養
基材で菌糸体を生成する前段階まで培養する。その培養
は１５℃乃至２２℃好しくは２０℃の暗条件下で行う。
このようにして培養された人工の冬虫夏草菌群を構成す
る個々の冬虫夏草菌は、野性で採用収穫された天然の冬
虫夏草菌と形態、形質において変わりがなく、従って、
この培養された人工の冬虫夏草菌は、さらに培養する二
次的元菌として容易に利用でき、さらに、培養基材で菌
糸体にまで生成された段階で培養を打ち切り、冬虫夏草
菌糸体としてエキス抽出原料とすることができ、そのエ
キスは調味料・ドリンク剤等の健康食品、漢方薬品等に
提供できるものである。Next, the Cordyceps sinensis inoculated in the previous step is cultivated up to the previous stage of forming mycelia on the culture substrate. The culture is carried out in the dark at 15 ° C to 22 ° C, preferably 20 ° C.
The individual Cordyceps fungi that make up the artificial Cordyceps fungus group cultivated in this manner are the same in morphology and traits as the natural Cordyceps fungus harvested in the wild, and therefore,
This cultured artificial Cordyceps sinensis can be easily used as a secondary source bacteria for further culturing, and further, the culture is discontinued at the stage when the mycelium is produced in the culture substrate, and the extract is used as an extract extract raw material as Cordyceps mycelium. The extract can be provided to health foods such as seasonings and drinks, and Kampo medicines.
【００１７】[0017]
【発明の効果】請求項１の発明の冬虫夏草の人工培養方
法によれば、冬虫夏草菌は本来の天然の寄主に比べ大量
の豊富な生育培養基材が与えられ、そこに感染し暗条件
下の所定温度に調整されて繁殖するからその冬虫夏草菌
を大量に人工培養でき、この人工培養の冬虫夏草菌を元
菌として、さらに、天然の冬虫夏草と同形態・同形質の
冬虫夏草を大量に人工栽培することができる。According to the method for artificially cultivating Cordyceps sinensis in accordance with the first aspect of the present invention, the Cordyceps fungus is provided with a large amount of an abundant growth culture substrate as compared with the natural host, which is infected therewith under dark conditions. Since the caterpillar fungus can be artificially cultivated in a large amount because it breeds after being adjusted to a predetermined temperature, it is possible to artificially cultivate a large amount of caterpillar fungus of the same morphology and trait as the natural caterpillar fungus as the original fungus. You can
【００１８】請求項２の発明によれば、天然の数少ない
冬虫夏草菌が一旦培養基材に接種されて繁殖すれば、こ
れを他の培養基材に接種するための元菌は豊富に存し容
易に取得できるから、これを順次繰り返し別の培養基材
に接種することが容易に、かつ迅速に行われ、冬虫夏草
の人工栽培は効率的に行える。この繁殖して得た冬虫夏
草菌は培養基材から分離し、継代している菌株たる元菌
として使用し譲渡できる他、そのまま菌糸体及び子実体
へ生育発展させることもできる。According to the second aspect of the present invention, once few natural natural Cordyceps sinensis are inoculated into a culture substrate and propagated, there are abundant original bacteria for inoculating this to other culture substrates. Therefore, it is possible to easily and rapidly inoculate this to another culture substrate in succession, and the artificial cultivation of Cordyceps sinensis can be efficiently performed. The Cordyceps sinensis obtained from this breeding can be separated from the culture substrate and used as the original strain that is the subcultured strain, and can be transferred to the mycelium or fruiting body.
【００１９】請求項３の発明に係る冬虫夏草の人工培養
基材によれば、同目または類縁目の昆虫の乾燥桶が寄主
として提供されることになり、あたかも本来の天然の宿
主が培地かの如く、冬虫夏草の元菌はその乾燥蛹によく
感染して増加繁殖する。According to the artificial culture substrate for Cordyceps sinensis according to the third aspect of the present invention, a dried tub of an insect of the same or related order is provided as a host, and it is as if the original natural host is a medium. As described above, the original fungus of Cordyceps often infects the dried pupae and multiply and multiply.
【００２０】また、請求項４の発明に係る冬虫夏草の人
工培養基材によれば、乾燥蚕蛹自体は繭の副産物として
コスト安で、かつ大量に購入できるから、冬虫夏草菌の
大量人工培養をより一層助長する。そして乾燥蚕蛹の大
量の需要は、衰退している蚕産業を復活させ得ることに
もなり、産業の発展の法目的に合致する。In addition, according to the artificial culture substrate for Cordyceps sinensis according to the invention of claim 4, dried silkworm pupae themselves are inexpensive as a by-product of cocoon and can be purchased in large quantities. Promote. And the large demand for dried silkworm pupae can also restore the declining silkworm industry, which is in line with the legal objectives of industrial development.
【００２１】さらに、請求項５の発明に係る冬虫夏草の
人工培養基材によれば天然の数少ない冬虫夏草菌の増加
繁殖を栄養源の供給により短期間に助長することができ
る。そして、さらに菌糸体の培養から子実体の生育へと
展開するときには、これらの収量を増大することができ
る。Further, according to the artificial culture substrate for Cordyceps sinensis according to the fifth aspect of the present invention, the increased breeding of few natural Cordyceps sinensis can be promoted in a short period of time by supplying a nutrient source. Then, when the process further progresses from the culture of mycelium to the growth of fruiting bodies, the yield of these can be increased.
【００２２】[0022]
【実験例１】人工栽培で得られる冬虫夏草が天然の冬虫
夏草と同一の形質に保たれているか否かを検証するため
抽出エキスをマウスに投与する下記の試験を行ったとこ
ろ、下表の成績を得た。 材料：乾燥蚕蛹を培養基材として人工培養したサナギタ
ケ培養菌糸体と野外で採集した天然のサナギタケ子実体
をそれぞれ乾燥後に熱水抽出した各エキス（以下、前者
を「人工エキス」、後者を「天然エキス」という）。 投与方法：２４ 月齢の老熟マウス雌各１０頭に１週間
隔で各エキス１ｍｌを腹腔内に注射した。なお、対照マ
ウスとしてエキス未投与の同齢のマウス雌１０頭を使用
した。 上記図表の結果から、人工栽培で得られる冬虫夏草の薬
効成分が天然の冬虫夏草のものと同形質に保持されてい
ることが判ると共に、これらを投与した場合は投与しな
い場合に比べ２倍以上の延命が実現でき、人間の寿命の
延長や制癌作用の奏功が示唆されているようである。[Experimental Example 1] In order to verify whether or not the Cordyceps sinensis obtained by artificial cultivation has the same trait as that of the natural Cordyceps sinensis, the following test was conducted in which the extract was administered to mice. Obtained. Ingredients: Each extract of hot-water extracted after drying dried pupa mycelium that was artificially cultured using dried silkworm pupae as a culture substrate and natural pupa fruit bodies collected in the field (hereinafter, the former is an "artificial extract" and the latter is a "natural extract"). "Extract"). Administration method: 10 ml of 24-month-old aged mice were intraperitoneally injected with 1 ml of each extract at weekly intervals. As control mice, 10 female mice of the same age to which the extract had not been administered were used. From the results in the above chart, it is clear that the medicinal components of artificially cultivated Cordyceps are retained with the same traits as those of natural Cordyceps, and when these are administered, the lifespan is extended by a factor of two or more compared to when they are not administered. It seems that it is possible to achieve the above, and it is suggested that the human life span is extended and the anti-cancer action is successful.
【００２３】[0023]
【実験例２】人工栽培で得られる冬虫夏草が天然の冬虫
夏草と同一の品質に保たれているか否かを検証するため
に食味テストを行い下表の結果を得た。 試験材料：前記実験例１と同様の人工エキス、天然エキ
ス。 試験方法：香り、味を５０人のモニターによって比較し
た。 上記図表の結果から、７割以上の者が人工エキス及び天
然エキスに食味上差がないとしており、冬虫夏草生産の
大量化・企業化による健康食品への提供に拒絶反応は少
なく、それが実現できることを示唆している。[Experimental Example 2] A tasting test was performed to verify whether the Cordyceps sinensis obtained by artificial cultivation is kept at the same quality as the natural Cordyceps sinensis, and the results shown in the following table were obtained. Test materials: artificial extracts and natural extracts similar to those of Experimental Example 1 above. Test method: Aroma and taste were compared by 50 monitors. From the results of the above chart, more than 70% of people said that there is no difference in taste between artificial extracts and natural extracts, and there are few rejection reactions to the provision of health foods due to mass production and industrialization of Cordyceps sinensis, which can be realized. It suggests.
【００２４】[0024]
【栽培例】製糸工場で排出された乾燥蚕輔１０００ｋｇ
を購入し、この乾燥蚕蛹に１００ｇ当たり１００ｍｌの
水道水を加え、これを１２０℃の蒸気中において常法で
２０分間かけて滅菌した後、これを培養基材として原野
で採集してきたサナギタケ即ち、スズメガの蛹に発生し
ていたサナギタケの子実体から分離、継代している菌株
を接種し、２０℃の暗条件で培養した。培養開始後３０
日に前記培養基材に菌糸体がまわった。この菌糸体１ｋ
ｇ（培養基材を含む）を８リットルの水と９８℃に加熱
して熱水抽出したところ５００ｇの人工エキスを得た。[Cultivation example] 1000 kg of dried silkworms discharged from the silk mill
Was purchased, and 100 ml of tap water per 100 g was added to this dried silkworm pupa, which was sterilized in steam at 120 ° C. for 20 minutes by a conventional method, and the pupa mushroom collected in the field as a culture substrate, that is, The strain isolated and subcultured from the fruit body of Pleurotus cornucopiae that had developed in the pupa of Suzumega was inoculated, and cultured under dark conditions at 20 ° C. 30 after the start of culture
The mycelium spread around the culture substrate daily. This mycelium 1k
When g (including the culture substrate) was heated to 8 liters of water and 98 ° C. for hot water extraction, 500 g of an artificial extract was obtained.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 (Ｃ１２Ｎ 1/14 Ｃ１２Ｒ 1:645） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification number Office reference number FI technical display location (C12N 1/14 C12R 1: 645)
Claims (5)
材に冬虫夏草の元菌を接種し、暗条件の所定温度で冬虫
夏草菌を培養させることを特徴とする冬虫夏草の人工培
養方法。1. A method for artificially cultivating Cordyceps sinensis, which comprises sterilizing a pupa-type culture matrix, inoculating the culture matrix with an original Cordyceps sinensis fungus, and culturing the Cordyceps sinensis at a predetermined temperature under dark conditions. .
の冬虫夏草菌叉は人工培養で生成した冬虫夏草菌である
ことを特徴とする請求項１記載の冬虫夏草の人工培養方
法。2. The method for artificially cultivating Cordyceps in accordance with claim 1, wherein the original Cordyceps fungus is a natural Cordyceps sinensis collected in the wilderness or a Cordyceps sinensis bacterium produced by artificial culture.
冬虫夏草菌の培地に調整したことを特徴とする冬虫夏草
の人工培養基材。3. An artificial culture substrate for Cordyceps sinensis, characterized in that the pupa of an insect that undergoes complete metamorphosis is dried and adjusted to a medium for Cordyceps sinensis.
得られることを特徴とする冬虫夏草の人工培養基材。4. An artificial culture substrate for Cordyceps sinensis, which is obtained by adjusting dried silkworm pupae to a medium for Cordyceps sinensis.
と、魚粉及び／叉は骨粉とを配合して冬虫夏草菌の培地
に調整したことを特徴とする冬虫夏草の人工培養基材。5. An artificial culture substrate of Cordyceps sinensis, which is prepared by blending silkworm pupa powder obtained by crushing dried silkworm pupae, fish meal and / or bone meal to prepare a medium for Cordyceps sinensis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6340819A JPH08172901A (en) | 1994-12-23 | 1994-12-23 | Method for carrying out artificial culture of cordyceps sinensis sacc. and artificial culturing substrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6340819A JPH08172901A (en) | 1994-12-23 | 1994-12-23 | Method for carrying out artificial culture of cordyceps sinensis sacc. and artificial culturing substrate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH08172901A true JPH08172901A (en) | 1996-07-09 |
Family
ID=18340593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6340819A Pending JPH08172901A (en) | 1994-12-23 | 1994-12-23 | Method for carrying out artificial culture of cordyceps sinensis sacc. and artificial culturing substrate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH08172901A (en) |
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|---|---|---|---|---|
| KR19990078866A (en) * | 1999-08-14 | 1999-11-05 | 김종수 | Artificial method of production for paecilomyces japonica by using the dried silkworm |
| JP2000201659A (en) * | 1999-01-14 | 2000-07-25 | Nichiharachiyou | Tochukaso tea |
| KR20010069295A (en) * | 2001-03-02 | 2001-07-25 | 이재일 | A method of cultivation Cordyceps militaris using a silkworm cocoon for culture medium |
| KR100361165B1 (en) * | 1999-07-03 | 2002-11-22 | 까치마을영농조합법인 | New Cordyceps sp. using Xylotrupes dichotomus larva as host and production thereof |
| KR100404333B1 (en) * | 2001-07-25 | 2003-11-03 | 에이치엔엠바이오(주) | A method of cultivating cordyceps based upon a snail or a mud snail |
| KR100407072B1 (en) * | 2001-08-06 | 2003-11-28 | 대한민국 | Artificial cultivation of Isaria sinclairii using the silkworm |
| KR100415893B1 (en) * | 2001-05-15 | 2004-01-24 | 주식회사 엔에스케이텍 | A process for isolation of cordyceps sinensis |
| KR20040007885A (en) * | 2002-07-11 | 2004-01-28 | 주식회사 엔에스케이텍 | Vegetable worms for strengthening vigor and its cultivation method |
| KR100478407B1 (en) * | 2002-11-28 | 2005-03-23 | 박동기 | The manufacturing metod of silk amino acid |
| JP2008054687A (en) * | 2007-10-05 | 2008-03-13 | Tsuwano Machi | Plant worm, plant worm tea, and food and drink |
| CN103563649A (en) * | 2013-10-10 | 2014-02-12 | 山东晨阳菌业有限公司 | Cultivation method of cordyceps militaris |
| CN105009941A (en) * | 2015-07-24 | 2015-11-04 | 刘随记 | Cordyceps sinensis continuous submerged fermentation liquor and fermentation powder production method |
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1994
- 1994-12-23 JP JP6340819A patent/JPH08172901A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000201659A (en) * | 1999-01-14 | 2000-07-25 | Nichiharachiyou | Tochukaso tea |
| KR100361165B1 (en) * | 1999-07-03 | 2002-11-22 | 까치마을영농조합법인 | New Cordyceps sp. using Xylotrupes dichotomus larva as host and production thereof |
| KR19990078866A (en) * | 1999-08-14 | 1999-11-05 | 김종수 | Artificial method of production for paecilomyces japonica by using the dried silkworm |
| KR20010069295A (en) * | 2001-03-02 | 2001-07-25 | 이재일 | A method of cultivation Cordyceps militaris using a silkworm cocoon for culture medium |
| KR100415893B1 (en) * | 2001-05-15 | 2004-01-24 | 주식회사 엔에스케이텍 | A process for isolation of cordyceps sinensis |
| KR100404333B1 (en) * | 2001-07-25 | 2003-11-03 | 에이치엔엠바이오(주) | A method of cultivating cordyceps based upon a snail or a mud snail |
| KR100407072B1 (en) * | 2001-08-06 | 2003-11-28 | 대한민국 | Artificial cultivation of Isaria sinclairii using the silkworm |
| KR20040007885A (en) * | 2002-07-11 | 2004-01-28 | 주식회사 엔에스케이텍 | Vegetable worms for strengthening vigor and its cultivation method |
| KR100478407B1 (en) * | 2002-11-28 | 2005-03-23 | 박동기 | The manufacturing metod of silk amino acid |
| JP2008054687A (en) * | 2007-10-05 | 2008-03-13 | Tsuwano Machi | Plant worm, plant worm tea, and food and drink |
| CN103563649A (en) * | 2013-10-10 | 2014-02-12 | 山东晨阳菌业有限公司 | Cultivation method of cordyceps militaris |
| CN105009941A (en) * | 2015-07-24 | 2015-11-04 | 刘随记 | Cordyceps sinensis continuous submerged fermentation liquor and fermentation powder production method |
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