KR20200029667A - A feed composition for the larva of protaetia brevitarsis using medicinal herb by-products and edible mushroom bacteria - Google Patents
A feed composition for the larva of protaetia brevitarsis using medicinal herb by-products and edible mushroom bacteria Download PDFInfo
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Abstract
Description
본 발명은 탕약재 제조 후 버려지는 한약재 부산물을 식용 버섯균인 느타리종균을 발효 촉진제로 활용하여 흰점박이꽃무지의 발육속도 및 생체중 증가 등의 생육 증대 효과 및 식품원료로서 안정성(중금속 및 유해물질 제거)을 확보할 수 있는 흰점박이꽃무지 유충 사료 조성물에 관한 것이다.The present invention utilizes the medicinal mushroom by-product, the edible mushroom by-product, which is discarded after the manufacture of the medicinal herbs, as a fermentation accelerator, increases the growth rate and increase in weight of white spotted flower radish and improves stability as a food raw material (removing heavy metals and harmful substances) It relates to a white spotted flower larva larva feed composition that can secure.
흰점박이꽃무지는 민간 및 동의보감 등의 전통 한방의서에서 "제조" 또는 "굼벵이" 라는 속명으로 불리어지고 있는 딱정벌레목, 풍뎅이과, 꽃무지아과에 속하며, 몸길이 17∼22㎜, 폭 12∼15㎜의 초식성 곤충으로, 몸은 진한 구리빛이고 광택이 있으며 황백색 무늬가 흩어져 있다. 흰점박이꽃무지는 오래 전부터 간질환 등의 치료를 위한 한방 약재로서 이용되어 왔으며, 최근에 유용한 생체 활성물질의 탐색 및 개발을 위한 곤충자원으로 크게 주목을 받고 있다. 또한, 흰점박이꽃무지는 흰쥐(mouse)를 이용한 실험에서 알코올 과량 섭취에 의해 손상된 간지질대사의 회복작용 등이 알려져 있으며, 혈전 용해성 효소에 대한 연구와 집쥐(rat)에서 사염화탄소의 투여에 의해 유도된 간독성에 대한 간보호효과를 나타내는 등 유용성이 확인된 바 있다.White-spotted flower radish belongs to the Coleoptera, Chafer, and Flower radish family, which are called "manufacturing" or "slugs" in traditional oriental medicines such as the folk and Donguibogam, body length 17-22 mm, width 12-15 mm Is a herbivorous insect of the body, the body is dark coppery, glossy, and has scattered yellowish-white patterns. White-spotted flower ignorance has long been used as a herbal medicine for the treatment of liver disease and the like, and has recently attracted great attention as an insect resource for exploration and development of useful bioactive substances. In addition, white spotted flower radish is known to recover the liver lipid metabolism damaged by excessive alcohol intake in experiments with mice, and it is induced by the study of thrombolytic enzymes and the administration of carbon tetrachloride in rats. Its usefulness has been confirmed, such as showing a liver protective effect on the liver toxicity.
현재 식품 원료로 인증받은 흰점박이꽃무지는 저지방 고단백과 다양한 영양성분을 포함하고 있으며, 간질환에 탁월한 효능이 있다는 것으로 알려져 있어 약용자원으로 활용이 되고 있다. 고급 단백질 공급원으로 알려져 있는 곤충자원인 흰점박이꽃무지 유충은 미생물에 의해 발효된 참나무 톱밥과 같은 부엽토 속에서 서식하며, 우리나라에서 약용으로 가장 많이 사육되고 있으나, 선별되지 않은 목재 부산물(폐목재, 건축폐기물 등)의 사료화로 흰점박이꽃무지 가공시 중금속 검출의 우려가 발생하여 식품의 안정성에 큰 문제가 되고 있다.White spotted flower radish, which is currently certified as a food ingredient, contains low-fat high-protein and various nutrients, and is known to have excellent effects on liver disease, and is used as a medicinal resource. White spotted flower larvae, an insect resource known as a high-grade protein source, inhabit the side-by-side soil such as oak sawdust fermented by microorganisms, and are the most cultivated for medicinal purposes in Korea, but unselected wood by-products (waste wood, construction Waste, etc.) has become a major problem in food stability due to concerns about detection of heavy metals when processing white spotted flower plain fabric.
이와 같이 매우 유용한 효능을 가진 흰점박이꽃무지를 사육하기 위한 사료에 대하여는 특허등록 제937152호 "뽕나무를 이용한 흰점박이꽃무지의 유충 및 성충의 사료 제조방법", 특허등록 제1719773호 "동충하초를 함유하는 꽃무지류 유충의 사료 및 이의 제조방법", 특허등록 제1623744호 "흰점박이꽃무지의 사료 조성물" 등과 같은 많은 기술이 출현하였다.For the feed for breeding white-spotted flower radish with such very useful efficacy, it contains patent registration No. 937152 "Method for producing larvae and adult feeds of white-spotted flower radish using mulberry", patent registration No. 1719773 A lot of techniques such as the feed of the flower larvae to be prepared and a method of manufacturing it, "Patent Registration No. 1623744," Feed composition of white spotted flower larvae "have appeared.
한편, 탕약재 제조 후 나오는 한약재 부산물은 퇴비화 하여 논밭에 거름으로 사용하면 산성화된 토양에 미생물이 되살아나고 유기질 함량이 증가한다고 알려져 있어서 활용되고 있고, 공개특허 제2002-8733호 "한약재 부산물을 이용한 사료"와 같은 기술도 출현하여 가금류의 사료로 일부 사용되고 있으며, 나머지는 많은 비용을 들여 폐기물로 처리하고 있다.On the other hand, the herbal medicine by-product produced after the manufacture of the medicinal herbs is used because it is known that microorganisms are revived in the acidified soil and the organic content increases when it is used as manure in paddy fields. The same technology has also emerged and is used as a feed for poultry, and the rest is treated as waste at a high cost.
본 발명의 출원인은 탕약재 제조 후 버려지는 한약재 부산물을 식용 버섯균인 느타리종균을 발효 촉진제로 활용하여 흰점박이꽃무지의 발육속도 및 생체중 증가 등의 생육 증대 효과 및 식품 원료로서 안정성(중금속 및 유해물질 제거)을 확보 할 수 있는 흰점박이꽃무지 유충의 사료에 대하여 많은 연구 개발을 한 끝에 본 발명에 이르게 되었다.The applicant of the present invention utilizes the medicinal mushroom by-product, edible mushroom fungus, as a fermentation accelerator, by using the medicinal by-product, which is discarded after the manufacture of the medicinal herbs, as a growth-promoting effect such as the growth rate and increase in weight of white spotted flower radish and stability as food ingredients (heavy metal and harmful After much research and development on the feed of white spotted flower larva larvae capable of securing substances), the present invention was reached.
본 발명이 해결하고자 하는 과제는, 탕약재 제조후 버려지는 한약재 부산물을 식용 버섯균인 느타리종균을 발효 촉진제로 활용하여 흰점박이꽃무지의 발육속도 및 생체중(larval weight) 증가 등의 생육 증대 효과 및 식품원료로서 안정성을 확보 할 수 있는, 한약재 부산물과 식용 버섯균을 이용한 흰점박이꽃무지 유충 사료 조성물을 제공하는 데 있다.The problem to be solved by the present invention is to use the medicinal mushroom by-product, edible mushroom fungus, as a fermentation accelerator, by using the by-product of herbal medicine discarded after the manufacture of the medicinal herb, and increase growth effects such as growth rate and increase in larval weight. It is to provide a composition of white spotted flower larva larvae using medicinal by-products and edible mushroom fungi that can secure stability as a food ingredient.
본 발명에 따른 흰점박이꽃무지 유충 사료 조성물은, 한약재 부산물을 식용 버섯균인 느타리종균으로 발효시킨 것을 특징으로 한다.The white spotted flower radish larva feed composition according to the present invention is characterized in that the medicinal by-products are fermented with edible mushroom bacteria, oyster species.
바람직하게는, 한약재 부산물은 십전대보탕 또는 쌍화탕의 부산물이다.Preferably, the by-product of the herbal medicine is a by-product of Siegjeondaebo-tang or Ssanghwa-tang.
바람직하게는, 한약재 부산물은 참나무발효톱밥과 혼합된 것이며, 한약재 부산물과 참나무발효톱밥의 혼합비율을 중량비로 2:8 내지 8:2이다.Preferably, the medicinal herb by-product is mixed with oak fermented sawdust, and the mixing ratio of the medicinal herb by-product and oak fermented sawdust is 2: 8 to 8: 2 by weight.
바람직하게는, 한약재 부산물은, 한약재를 120℃, 1.2기압에서 중탕하는 단계; 중탕 추출액의 액상을 완전 제거하고 남은 찌꺼기인 부산물을 70℃에서 12시간 열풍 건조기를 이용하여 건조하는 단계; 건조 후 50메쉬의 미세분말 형태로 파쇄하는 단계를 거쳐서 제작된 것이다.Preferably, the by-product of the herbal medicine, step of bathing the herbal medicine at 120 ℃, 1.2 atmospheres; Completely removing the liquid phase of the heavy bath extract and drying the remaining by-products at 70 ° C. for 12 hours using a hot air dryer; After drying, it was produced through the step of crushing into a fine powder form of 50 mesh.
본 발명은 폐기가 곤란하고, 처리에 많은 비용을 요하는 한약재 부산물을 이용하여 흰점박이꽃무지 유충의 사료로 사용함으로써 유충의 생존율 및 생체량을 향상시킬 수 있는 효과가 있다. 또한, 본 발명에 따른 사료 조성물은 각종 영양분이 풍부한 한약재 부산물을 사용하기 때문에 본 발명의 사료 조성물을 먹으면서 사육된 흰점박이꽃무지 유충의 영양 성분의 함량 증가를 기대할 수 있고, 흰점박이꽃무지 유충의 약리적 효과를 현저하게 향상시킬 수 있다.The present invention has the effect of improving the survival rate and biomass of the larvae by using it as a feed for white spotted flower larvae by using a by-product of medicinal herbs that are difficult to dispose and require a large cost for treatment. In addition, since the feed composition according to the present invention uses a medicinal herb byproduct rich in various nutrients, it is possible to expect an increase in the content of nutrients of the white spotted flower larvae raised while eating the feed composition of the present invention, and The pharmacological effect can be significantly improved.
도 1는 한약재 부산물 첨가 비율에 따른 흰점박이꽃무지 유충의 생체중에 대한 그래프이다.
도 2는 한약재 부산물 첨가 비율에 따른 흰점박이꽃무지 유충의 생존율에 대한 그래프이다.
도 3a는 EM균 발효 한약재 첨가 먹이조건에서의 흰점박이꽃무지 유충의 성장 사진이다.
도 3b는 느타리종균 발효 한약재 첨가 먹이조건에서의 흰점박이꽃무지 유충의 성장 사진이다.
도 4는 EM균과 느타리종균 발효 한약재 40% 첨가 먹이조건에서의 흰점박이꽃무지 유충의 증체량의 그래프이다.
도 5는 EM균과 느타리종균 발효 한약재 80% 첨가 먹이조건에서의 흰점박이꽃무지 유충의 증체량의 그래프이다.
도 6은 EM균과 느타리종균 발효 한약재 100% 첨가 먹이조건에서의 흰점박이꽃무지 유충의 증체량의 그래프이다.Figure 1 is a graph of the weight of the white spotted flower larva larvae according to the ratio of by-products of herbal medicines.
Figure 2 is a graph of the survival rate of white spotted flower larvae larvae according to the rate of addition of by-products of medicinal herbs.
Figure 3a is a photograph of the growth of white spotted flower larva larvae in the feeding conditions with the addition of EM fermented medicinal herbs.
Figure 3b is a photograph of the growth of white spotted flower larva larvae in feeding conditions with the addition of medicinal herbs fermented with oyster seed.
Figure 4 is a graph of the increase in the amount of white spotted flower larvae larvae under the feeding conditions of 40% fermented herbal medicines of EM and Oysteria spp.
Figure 5 is a graph of the increase in the amount of white spotted flower larvae larvae in the feeding conditions with 80% of the fermented herbal medicines of EM and Oysteria spp.
Figure 6 is a graph of the increase in the amount of white spotted flower larvae larvae under the feeding conditions of 100% herbal medicines fermented by EM bacteria and oyster species.
본 발명은 한약재 부산물을 유효성분으로 함유하는 흰점박이꽃무지 유충 사료 조성물을 제공하기 위한 것으로, 한약재 부산물로는 특히 십전대보탕, 쌍화탕의 주원료인 당귀, 감초, 작약, 숙지황, 황기 등이 혼합되어 있는 한약재를 사용할 수 있다.The present invention is to provide a white spotted flower larvae larva feed composition containing a medicinal herb by-product as an active ingredient, as a medicinal herb by-product, in particular, the main raw material of Siegjeondaebotang, Ssanghwatang, Licorice, Licorice, Peony, Sukjihwang, Hwanggi, etc. are mixed. You can use herbal medicine.
상기 사료 조성물은 한약재 부산물과 식용 버섯균인 느타리종균을 발효 촉진제를 포함할 수 있다. 또한, 상기 사료 조성물은 한약재 부산물을 참나무발효톱밥과 혼합하여 사용할 수도 있는데, 이때 한약재 부산물과 참나무발효톱밥의 혼합비율은 2:8 내지 8:2의 중량비로 혼합된다.The feed composition may include a fermentation accelerator for medicinal byproducts and edible mushroom bacteria, oyster spp. In addition, the feed composition may be used by mixing the medicinal herbs by-products with oak fermented sawdust, wherein the mixing ratio of the medicinal herbs by-products and oak fermented sawdust is mixed in a weight ratio of 2: 8 to 8: 2.
[실시예1][Example 1]
<한약재 부산물의 제조><Preparation of herbal medicine by-products>
본 발명의 한약재 부산물은 탕 제원에서 보양을 위해 흔하게 사용되는 십전대보탕, 쌍화탕의 주원료인 당귀(20중량%), 감초(20중량%), 작약(20중량%), 숙지황(20중량%), 황기(20중량%)의 한약재를 120℃, 1.2기압에서 중탕을 실시한다. 그 후, 중탕 추출액의 액상을 완전 제거하고 남은 찌꺼기인 한약재 부산물을 수거하여 수분제거를 위해 70℃에서 12시간 열풍건조기를 이용하여 건조한다. 완전 건조 후 50메쉬의 핀밀분쇄기를 사용 미세분말 형태로 파쇄하여 제조한다.The by-product of the medicinal herbs of the present invention is the main raw material of Sipjeondaebo-tang, Ssanghwa-tang (20% by weight), licorice (20% by weight), peony (20% by weight), peony (20% by weight), sujijihwang (20% by weight), which are commonly used for rejuvenation in the Tang specifications. The herbal medicine of Hwanggi (20% by weight) is boiled at 120 ° C and 1.2 atmospheres. Thereafter, the liquid phase of the heavy-water extract is completely removed, and the remaining residues of medicinal herbs are collected and dried using a hot air dryer at 70 ° C for 12 hours to remove moisture. After complete drying, it is manufactured by crushing a 50-mesh pin mill grinder into fine powder.
<한약재 부산물의 영양성분><Nutrition components of herbal medicine by-products>
흰점박이꽃무지 사육농가에서 주로 사용하는 방법으로 참나무톱밥에 EM 발효균을 활용하여 발효시킨 참나무발효톱밥과, 본 발명의 한약재 부산물, 증류수로 발효시킨 한약재 부산물의 영양성분의 조단백질, 조섬유, 조지방, 조회분을 측정하여 [표 1]에 나타내었다.Crude protein, crude fiber, crude fat, lookup of nutritional components of oak fermented sawdust fermented by using EM fermentation bacteria in oak sawdust as a method mainly used in white spotted flower plain breeding farms, and by-products of the medicinal herbs of the present invention and medicinal herbs by fermented with distilled water The minutes were measured and shown in [Table 1].
[표 1]과 같이, 조섬유를 제외한 조단백, 조지방, 조회분 등에서 한약재 부산물 미발효, 발효조건 모두에서 EM균 발효 참나무톱밥보다 높게 나타났으며, 특히 조단백질의 함량이 매우 높게 나타났다. 이는 단백질의 경우 곤충의 성장과 생체중 증가에 영향을 준다는 기존 보고와 같이 한약재 부산물의 급이에 따라 흰점박이꽃무지 유충의 성장에 있어서 생체중의 증가와 생장기간 단축에 효과가 있을 것으로 판단된다.As shown in [Table 1], the crude protein, crude fat, and ash powder, except crude fiber, showed higher fermentation conditions than those of EM-fermented oak sawdust in both fermentation and non-fermentation by-products of medicinal herbs. In particular, the content of crude protein was very high. This is considered to be effective in increasing the live weight and shortening the growth period in the growth of white spotted flower larva larvae according to the supply of medicinal herbs by-product, as in the previous report that the protein affects insect growth and increase in live weight.
또한, 한약재 부산물의 발효와 미발효조건을 비교한 결과 발효 한약재 부산물의 경우 조지방을 제외하고 조단백과 조섬유, 조회분 등이 증가하는 것으로 나타났다. 이는 발효 미생물의 성장에 따라 단백질의 함량 및 조섬유, 조회분의 증가로 판단되며 흰점박이꽃무지 유충의 경우 부숙된 유기물을 먹이로 이용하는 습성이 있어 발효조건에 따른 성장에 큰 영향을 줄 것으로 판단된다.In addition, as a result of comparing the fermentation and non-fermentation conditions of by-products of medicinal herbs, it was found that in the case of fermented medicinal herbs by-products, crude protein, crude fiber, and ash content increased except for crude fat. This is judged to increase the protein content, crude fiber, and ash content according to the growth of fermented microorganisms. In the case of white spotted flower larvae, it is thought to have a great influence on growth according to fermentation conditions because of the habit of using unripe organic matter as food. .
<먹이원에 따른 흰점박이꽃무지의 성장 비교><Comparison of growth of white spotted flower ignorance according to feed source>
흰점박이꽃무지 사육 농가에서 먹이 제조에 일반적으로 사용하고 있는 EM균을 이용하여 건조 파쇄된 한약재 부산물에 중량대비 20%의 비율로 EM균(x1010cfu 이상)을 첨가하고, EM균이 첨가된 한약재부산물은 잘 혼합한 후 상온 25℃에서 30일간 발효공정을 실시하였다.EM bacteria (x10 10 cfu or more) are added at a ratio of 20% by weight to dry crushed herbal by-products using EM bacteria that are commonly used for food production at white-spotted flower plains farms, and EM bacteria are added After mixing well with the medicinal herbs, fermentation was performed at room temperature of 25 ℃ for 30 days.
[표 2]와 같이 30일간 완전히 발효된 한약재 부산물을 기존 농가에서 관행적으로 사용하는 참나무발효톱밥(EM균 발효)에 중량비율로 40%, 80%, 100% 비율로 첨가 혼합하여 먹이 제조하였다.As shown in [Table 2], 40%, 80%, and 100% of the ratio was added to the oak fermented sawdust (EM-fermented fermentation) conventionally used in existing farms by fermentation of herbal medicines that were fully fermented for 30 days. .
각각의 비율로 제조된 먹이를 흰점박이꽃무지 1령 유충에게 급이를 실시하였고, 실험은 처리군당 10마리씩 3반복 실험 실시하였으며, 처리군간 생육속도를 비교하기 위해 주 1회 전자저울을 이용하여 무게를 측정하였다. 한약재 부산물 첨가 비율에 따른 흰점박이꽃무지 유충의 생체중은 도 1의 그래프로 나타내었고, 한약재 부산물 첨가 비율에 따른 흰점박이꽃무지 유충의 생존율은 도 2의 그래프로 나타내었다.Food prepared at each ratio was fed to 1 larvae of white spotted flower plain, and the experiment was conducted in 3 replicates of 10 animals per treatment group, and an electronic balance was used once a week to compare the growth rate between treatment groups. The weight was measured. The live weight of the white spotted flower larvae according to the rate of addition of the herbal medicine by-product is shown in the graph of FIG. 1, and the survival rate of the white spotted flower larvae according to the rate of the addition of the oriental medicine by-product is shown in the graph of FIG. 2.
실험 결과, 도 1 및 도 2에서 확인할 수 있듯이, 대조구(참나무발효톱밥 100%)대비 한약재 부산물 80% 첨가비율이 556% 이상 증체효과를 나타냈으며, 100% 한약재 부산물의 경우 초기 적응 실패로 치사율이 높았으나, 점차 안정적인 생육을 보였으며, 증체율에 있어서도 500%이상 증체하는 것을 확인할 수 있었다.As shown in FIG. 1 and FIG. 2, 80% addition rate of 80% herbal by-products compared to the control (Oak Fermented Sawdust) showed a 556% or more increase, and 100% Chinese herbal by-products had a fatality rate due to initial adaptation failure. Although it was high, it showed stable growth gradually, and it was confirmed that it increased more than 500% in the growth rate.
<발효균주의 종류에 따른 흰점박이꽃무지 유충의 성장 비교><Comparison of the growth of white spotted flower larvae according to the type of fermentation strain>
흰점박이꽃무지 사육 농가에서 먹이 제조에 일반적으로 사용하고 있는 EM균과 식용 버섯균으로 많이 알려진 느타리버섯 종균을 이용하여 건조 파쇄된 한약재 부산물에 중량대비 20%의 비율로 각각의 EM균, 느타리버섯종균 액상배양액(x1010cfu 이상)을 첨가하여 잘 혼합한 후 상온 25℃에서 30일간 발효공정을 실시하였다. 식용 버섯균인 PD(Potato dextros)배지를 이용하여 28℃에서 7일간 액상 배양시킨 느타리버섯 종균을 활용하였다. Each EM bacterium and oyster mushroom in a ratio of 20% by weight to the by-products of dried crushed herbal medicine using EM bacteria commonly used for food production at farms where white-spotted flowers are bred and oyster mushroom species commonly known as edible mushroom bacteria After adding the seed culture liquid (x10 10 cfu or more) and mixing well, the fermentation process was performed at room temperature of 25 ° C for 30 days. Using the edible mushroom fungus PD (Potato dextros) medium, a strain of oyster mushroom cultured at 28 ° C for 7 days was used.
[실시예2][Example 2]
<발효 한약재 부산물의 영양성분><Nutrition component of fermented herbal medicine by-products>
발효 미생물의 첨가에 따른 한약재 부산물의 영양성분의 조단백질, 조섬유, 조지방, 조회분을 측정하여 [표 3]에 나타내었고, 비교예로 참나무톱밥에 EM 발효균을 활용하여 발효시킨 참나무발효톱의 영양성분을 함께 나타내었다. Measurement of crude protein, crude fiber, crude fat, and ash content of nutritional components of herbal medicine by-products according to the addition of fermented microorganisms is shown in [Table 3], and as a comparative example, nutritional components of oak fermented saws fermented using EM fermentation bacteria in oak sawdust Together.
[표 3]과 같이, 발효 균주의 조건에 따라 한약재 부산물의 영양성분의 경우 조회분을 제외하고 조단백, 조섬유, 조지방 등의 수치가 느타리버섯 종균으로 발효시킨 한약재 부산물에서 높게 나타났다. 또한, 발효된 한약재 부산물은 발효 미생물의 종류와 상관없이 참나무 발효톱밥과 비교했을 때 조섬유를 제외하고 모든 수치가 높게 나타나는 것을 확인하였으며, 특히 조단백질의 경우 차이가 매우 큰 것으로 나타났다.As shown in [Table 3], according to the conditions of the fermentation strain, the nutritional components of by-products of herbal medicines were higher in the by-products of herbal medicines fermented with the seeds of oyster mushroom, except crude ash. In addition, the fermented herbal medicines by-products were found to have high levels except for crude fibers, especially when compared to oak fermented sawdust, regardless of the type of fermented microorganisms, especially in the case of crude protein.
[실시예3][Example 3]
[표 4]와 같이 완전히 발효된 한약재 부산물을 기존 농가에서 관행적으로 사용하는 참나무발효톱밥(EM균 발효)에 중량비율로 40%, 80%, 100% 비율로 첨가 혼합하여 흰점박이꽃무지 1령 유충에게 급이를 실시하였다. 실험은 처리군당 10마리씩 3반복 실험 실시하였고, 처리군간 생육속도를 비교하기 위해 주 1회 전자저울을 이용하여 무게 측정하였다. As shown in [Table 4], white fermented flower solid 1 by adding 40%, 80%, 100% by weight ratio of oak fermented sawdust (EM-fermented fermentation) conventionally used in existing farms by using fermented herbal medicine by-
<발효균주별 한약재 부산물 첨가비율에 따른 유충의 성장 비교><Comparison of larva growth according to the ratio of the addition of by-products of medicinal herbs by fermentation strain>
EM균과 느타리종균 발효 한약재 첨가 먹이조건에서의 한약재 부산물 첨가비율에 따른 유충의 성장사진, 유충의 증체량을 각각 확인하여 도 3a, 3b의 사진, 도 4, 도 5 및 도 6의 그래프로 나타내었다. 여기서 도 3a는 EM균 발효 한약재 첨가 먹이조건에서의 흰점박이꽃무지 유충의 성장사진이고, 도 3b는 느타리종균 발효 한약재 첨가 먹이조건에서의 흰점박이꽃무지 유충의 성장사진이다. 또한, 도 4는 EM균과 느타리종균 발효 한약재 40% 첨가 먹이조건에서의 흰점박이꽃무지 유충의 증체량의 그래프이고, 도 5는 EM균과 느타리종균 발효 한약재 80% 첨가 먹이조건에서의 흰점박이꽃무지 유충의 증체량의 그래프이고, 도 6은 EM균과 느타리종균 발효 한약재 100% 첨가 먹이조건에서의 흰점박이꽃무지 유충의 증체량의 그래프이다.Addition of fermented medicinal herbs fermented by EM bacteria and oyster species, photographs of larva growth according to the ratio of medicinal byproducts added under feeding conditions, and the increase of larvae respectively, are shown in the graphs of FIGS. 3A, 3B, 4, 5, and 6 . Here, FIG. 3A is a photograph of growth of white spotted flower larva larvae under the feeding conditions with the addition of EM-fermented medicinal herbs, and FIG. 3B is a photograph of growth of white-spotted flower larvae larvae under the feeding conditions with the fermented Chinese medicine. In addition, Figure 4 is a graph of the increase in the amount of white spotted flower larvae larvae in the feeding conditions of 40% fermented herbal medicines of EM bacteria and oyster species, Figure 5 is white spotted flowers in the feeding conditions of 80% of EM bacteria and oyster species fermented herbal medicines It is a graph of the weight gain of the plain larvae, and FIG. 6 is a graph of the weight increase of the white spotted flower larva larva under the feeding conditions with 100% of the fermented herbal medicines of EM and Oyster strains.
도 3a, 3b의 사진, 도 4, 도 5 및 도 6의 그래프와 같이, 느타리 종균을 이용한 발효 한약재의 경우 EM균을 이용한 발효 한약재와 비교해서 약 116% ∼ 200% 이상의 성장 속도에 차이가 있는 것으로 나타났으며, 참나무 발효톱밥과의 첨가 비율이 증가할수록 느타리 종균을 이용한 한약재 부산물 발효 조건에서 EM균 발효조건보다 월등히 높은 생체중 증가 효과가 있는 것으로 확인할 수 있었다. 즉, 기존 참나무발효톱밥과의 혼합재로서 한약재 부산물을 활용하는 것이 경제적이면서 성장속도를 빨리할 수 있어 효과가 우수하다고 판단할 수 있다.3A, 3B, as shown in the graphs of FIGS. 4, 5, and 6, in the case of fermented herbal medicines using oyster spawn, there is a difference in growth rate of about 116% to 200% or more compared to fermented herbal medicines using EM bacteria It was confirmed that as the addition rate of oak fermented sawdust increased, the fermentation condition of herbal medicines using oyster spp. Was significantly higher than that of EM bacteria. In other words, it can be judged that it is economical to use a by-product of medicinal herbs as an admixture with the existing oak fermented sawdust, and it can be judged to be excellent in effect because it can speed up the growth.
<발효균주별 한약재 부산물 첨가비율에 따른 유충의 생존율 비교><Comparison of survival rate of caterpillars according to the rate of addition of by-products of medicinal herbs by fermenting bacteria>
EM균과 느타리종균 발효 한약재 첨가 먹이조건에서의 한약재 부산물 첨가비율에 따른 유충의 생존율은 [표 5]에 나타내었다.The survival rate of larvae according to the ratio of addition of by-products of medicinal herbs under the feeding conditions with the addition of EM and Fermented Oriental Medicinal Herbs is shown in [Table 5].
[표 5]와 같이, 한약재 부산물 첨가농도에 따른 유충의 생존율을 조사한 결과 한약재 부산물의 첨가농도가 높을수록 생존율이 떨어지는 결과를 확인할 수 있었다. 특히 1령 초기 치사율이 급격하게 증가하는 현상을 확인할 수 있었으며, 성장이 증가할수록 치사율은 변동이 없는 것으로 관찰되어, 한약재 부산물의 발효가 제대로 이루어지지 않아 급이 이후에 후 발효 등의 원인으로 판단된다.As shown in [Table 5], as a result of investigating the survival rate of the larva according to the concentration of by-product of the herbal medicine, the higher the concentration of the by-product of the herbal medicine, the lower the survival rate. In particular, it was confirmed that the mortality rate in the early stage of 1st year rapidly increased, and as the growth increased, the mortality rate was observed to remain unchanged. .
<발효균주별 한약재 부산물 첨가비율에 따른 용화율 비교><Comparison of solubility according to the ratio of the addition of by-products of herbal medicines by fermentation strain>
EM균과 느타리종균 발효 한약재 첨가 먹이조건에서의 한약재 부산물 첨가비율에 따른 흰점박이꽃무지의 용화율은 [표 6]에 나타내었다.The solubilization rate of white spotted flower radish according to the ratio of the addition of herbal medicines by-products under the feeding conditions of fermented herbal medicines of EM and Oyster strains is shown in [Table 6].
한약재 부산물 40%Oak fermented
40% by-product of herbal medicine
한약재 부산물 80%Oak fermented
80% by-product of herbal medicine
한약재 부산물 100%
100% by-product of herbal medicine
[표 6]과 같이, 한약재 부산물을 EM균과 느타리종균을 이용하여 발효를 시킨 후 참나무발효톱밥과 비율별 첨가에 따른 흰점박이꽃무지의 용화율을 비교한 결과 대조구인 참나무발효톱밥의 경우 유충이 번데기에 들어가는 기간이 12주가 소요되는 반면, EM균과 느타리종균 발효조건으로 만들어진 한약재 부산물의 경우 번데기에 들어가는 기간이 6∼8주정도 소요되어 빠른 성장을 확인할 수 있었으며, 용화율에 있어서도 참나무발효톱밥보다 모두 우수하였다. 또한, 발효 미생물의 종류에 따른 용화율을 비교했을때 전반적으로 느타리종균을 접종한 것이 우수한 것으로 나타났다. As shown in [Table 6], after fermentation of herbal medicine by-products using EM bacteria and oyster species, the solubility rate of white spotted flower radish according to the addition of oak fermented sawdust and ratio is compared to that of the control group, oak fermented sawdust. While the period for entering this pupa takes 12 weeks, in the case of by-products of herbal medicines made under the fermentation conditions of EM and oyster species, the period for entering the pupa takes about 6 to 8 weeks, and it is possible to confirm the rapid growth. All were better than sawdust. In addition, when comparing the solubility according to the type of fermented microorganisms, it was found that the inoculum was inoculated overall.
<발효균주별 한약재 부산물 첨가비율에 따른 우화율 비교><Comparison of fable rate according to the rate of addition of by-products of medicinal herbs by fermentation strain>
EM균과 느타리종균 발효 한약재 첨가 먹이조건에서의 한약재 부산물 첨가비율에 따른 흰점박이꽃무지의 우화율은 [표 7]에 나타내었다.Fermentation rate of white spotted flower radish according to the ratio of the addition of herbal medicines by-products under the feeding conditions of fermented herbal medicines of EM bacteria and oyster seed fermentation is shown in [Table 7].
한약재 부산물 40%Oak fermented
40% by-product of herbal medicine
한약재 부산물 80%Oak fermented
80% by-product of herbal medicine
한약재 부산물 100%
100% by-product of herbal medicine
[표 7]과 같이, 우화율에 있어서도 참나무발효톱밥의 경우 번데기 기간은 4주, 우화율은 63%인 반면, EM균의 경우 67∼79%의 우화율을 나타내고 있으며, 느타리종균의 경우 70∼77%의 우화율을 나타내고 있어, EM균과 느타리종균의 우화율에 대한 차이는 없는 것으로 나타났다.As shown in [Table 7], in the case of oak fermentation, the pupal period for oak fermented sawdust is 4 weeks, and the fertilization rate is 63%, whereas for the EM bacteria, it shows the fertilization rate of 67 to 79%. It showed a fable rate of ∼77%, so it was found that there was no difference in the fable rate between the EM and Oyster species.
이상의 설명은 본 발명을 예시적으로 설명한 것이고, 명세서에 게시된 실시예는 본 발명의 기술사상을 한정하기 위한 것이 아니라 설명하기 위한 것이므로 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 본 발명의 기술사상을 벗어나지 않는 범위에서 다양한 수정 및 변형이 가능할 것이다. 그러므로 본 발명의 보호범위는 청구범위에 기재된 사항에 의해 해석되고, 그와 균등한 범위 내에 있는 기술적 사항도 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The above description is illustrative of the present invention, and the embodiments disclosed in the specification are not intended to limit the technical idea of the present invention, but are intended to explain the present invention. Various modifications and variations will be possible without departing from the technical idea of. Therefore, the protection scope of the present invention is to be interpreted by the matters described in the claims, and technical matters within the equivalent scope thereof should be interpreted to be included in the scope of the present invention.
Claims (5)
Composition of white spotted flower larva larva using medicinal by-products and edible mushrooms, characterized by fermenting the by-products of medicinal herbs as edible mushrooms.
상기 한약재 부산물은 십전대보탕 또는 쌍화탕의 부산물인 것을 특징으로 하는 한약재 부산물과 식용 버섯균을 이용한 흰점박이꽃무지 유충 사료 조성물.
The method according to claim 1,
The medicinal herb by-product is a composition of white spotted flower larva larva using medicinal mushroom and edible mushroom fungus, characterized in that a by-product of Siegjeondaebo-tang or Ssanghwa-tang.
상기 한약재 부산물은 참나무발효톱밥과 혼합되는 것을 특징으로 하는 한약재 부산물과 식용 버섯균을 이용한 흰점박이꽃무지 유충 사료 조성물.
The method according to claim 1 or claim 2,
The herbal medicine by-product is a white spotted flower larva larva feed composition using medicinal mushroom by-product and medicinal mushroom by-product, characterized in that mixed with oak fermented sawdust.
상기 한약재 부산물과 참나무발효톱밥의 혼합비율을 중량비로 2:8 내지 8:2인 것을 특징으로 하는 한약재 부산물과 식용 버섯균을 이용한 흰점박이꽃무지 유충 사료 조성물.
According to claim 3,
The composition of the white medicinal flower larvae using medicinal mushrooms and edible mushroom bacteria, characterized in that the mixture ratio of the herbal medicine by-product and oak fermented sawdust is 2: 8 to 8: 2 by weight.
한약재를 120℃, 1.2기압에서 중탕하는 단계;
중탕 추출액의 액상을 완전 제거하고 남은 찌꺼기인 부산물을 70℃에서 12시간 열풍 건조기를 이용하여 건조하는 단계;
건조 후 50메쉬의 미세분말 형태로 파쇄하는 단계;
를 거쳐서 제작된 것을 특징으로 하는 한약재 부산물과 식용 버섯균을 이용한 흰점박이꽃무지 유충 사료 조성물.The method according to claim 1, The by-product of the herbal medicine
Bathing the herbal medicine at 120 ° C and 1.2 atmospheres;
Completely removing the liquid phase of the heavy bath extract and drying the remaining residue, byproducts, at 70 ° C. for 12 hours using a hot air dryer;
Crushing in the form of a fine powder of 50 mesh after drying;
White spotted flower larva larva feed composition using medicinal by-products and edible mushroom fungi, characterized by being produced through.
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