JPH07505042A - パピローマウイルスワクチン - Google Patents
パピローマウイルスワクチンInfo
- Publication number
- JPH07505042A JPH07505042A JP5502486A JP50248693A JPH07505042A JP H07505042 A JPH07505042 A JP H07505042A JP 5502486 A JP5502486 A JP 5502486A JP 50248693 A JP50248693 A JP 50248693A JP H07505042 A JPH07505042 A JP H07505042A
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- protein
- recombinant
- virus
- proteins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.(i)パピローマウイルスL1蛋白あるいはパピローマウイルスL1蛋白お よびパピローマウイルスL2蛋白の組合せをコードする1またはそれを超る組換 えDNA分子を構築し;次いで (ii)L1またはL1およびL2蛋白の組合せの発現の後に細胞内でウイルス 様粒子(VLP)が産生されるように、該1またはそれを超る組換えDNA分子 で適当な宿主細胞をトランスフェクトする工程を含むことを特徴とするパピロー マウイルス様粒子の生産方法。 2.L1およびL2蛋白をコードするDNA分子が同一のDNA分子包含される 請求の範囲第1項記載の方法。 3.L1およびL2蛋白をコードするDNA分子が異なるDNA組換え分子に包 含される請求の範囲第1項記載の方法。 4.工程(ii)において、1またはそれを超える組換えDNA分子を含有する 組換えウイルスが、宿主細胞をトランスフェクトする請求の範囲第1項記載の方 法。 5.工程(i)において、該組換えDNA分子を含有する一次プラスミドを形成 させ、続いて、これで、野生型ウイルスで既に感染させた宿主細胞を感染させて 該組換えウイルスを得る請求の範囲第4項記載の方法。 6.該一次プラスミドが、工程(ii)においてL1またはL1およびL2蛋白 の発現を促進するプロモーターに連結した該組換えDNA分子を含有する請求の 範囲第5項記載の方法。 7.該一次プラスミドの形成に先立ち、該L1またはL2蛋白をコードする遺伝 子を、一次プラスミドの形成前に、核プロモーターを含有する二次プラスミドに 挿入する請求の範囲第5項記載の方法。 8.L1蛋白をコードする該組換えDNA分子を含有する該一次プラスミドがそ こに挿入されたし2蛋白をコードするさらなる組換えDNA分子を有して、二重 組換え発現プラスミドを形成させ、しかる後、一次プラスミドおよび二重組換え 発現プラスミドで、野生型ウイルスで感染させた宿主細胞を感染させる請求の範 囲第5項記載の方法。 9.該組換えウイルスがスポドブテラ・フルギペルダ(Spodoptera frugiperda)のごとき昆虫細胞を感染させるバクロウイルスに由来す る請求の範囲第4項記載の方法。 10.該組換えウイルスがワクシニアウイルスに由来する請求の範囲第4項記載 の方法。 11.L1蛋白をコードする遺伝子を、(i)強力なワクシニアウイルスプロモ ーター4bを含有するプラスミドに組み込み、(ii)該4bプロモーターに連 結した遺伝子を含有するDNA断片が該プラスミドに由来し、かつ(iii)そ の結果、ワクシニアウイルスのB24R遺仏子およびイー・コリ(E.coli )gpt遺伝子および多重クローニング部位を含有するワクチン中間体ペクター に該DNA断片を挿入してプラスミドpLC200を得る請求の範囲第10項記 載の方法。 12.28Kプロモーターに連結したL2遺伝子を含有する断片をプラスミドp LCに挿入する前に、L2蛋白をコードする遺伝子を、合成ワクシニア28K後 期プロモーターを有するpUC由来プラスミドに組み込んでプラスミドpLC2 01を得る請求の範囲第11項記載の方法。 13.プラスミドpLC201を利用して組換えワクシニアウイルスpLC20 1VVを構築し、これで引き続いて哺乳動物細胞を感染させて蛋白L1およびL 2を含有するVLPを得る請求の範囲第12項記載の方法。 14.BPV L1蛋白をコードする遺伝子を含有する組換えDNA分子を構築 し、PCR増幅後に、ワクシニア4bプロモーターに連結させ、しかる後、該D NA組換え分子をプラスミドpSX3に挿入してプラスミドpSXBPVL1が 得られ、しかる後、これで、野生型ワクシニアウイルスで感染させた宿主細胞を トランスフェクトさせてrVV pSXBPVL1が得られ、宿主細胞の引き続 いてのトランスフェクションの後にBPV L1蛋白を含有するVLPが生産さ れる請求の範囲第1項記載の方法。 15.PCR増幅後に、合成ワクシニア28K後期プロモーターに連結したBP V L2蛋白をコードする遺伝子を含有する組換えDNA分子を構築して(その 組換えDNA分子はプラスミドpSXBPVL1への挿入前にはプラスミドp4 80 BPVL2であり)プラスミドpSXBPVL1L2が得られ、しかる後 、これで、野生型ワクシニアウイルスで感染させた宿主細胞をトランスフェクト させてBPV L1蛋白を含有するVLPを得る請求の範囲第14項記載の方法 。 16.PCR増幅の後に、ワタシエア4bプロモーターに連結したHPV11L 1蛋白をコードする遺伝子を含有する組換えDNA分子を構築し、しかる後、該 DNA組換え分子をプラスミドpSX3に挿入してプラスミドpSX11L1が 得られ、しかる後、これで、宿主細胞を感染させてL1蛋白を含有するHPV1 1 VLPを得る請求の範囲第1項記載の方法。 17.PCR増幅の後に、ワクシニア4bプロモーターに連結したHPV11L 2蛋白を−ドする遺伝子を含有する組換えDNA分子を構築し、しかる後、該組 換えDNA分子をワクシニア28K後期プロモーターを含有するプラスミドp4 80に挿入し、引き続いて、該プロモーターに連結した該遺伝子を含有するDN A断片をプラスミドpSX11L1に移してプラスミドpSX11L1L2が得 られ、しかる後、これで宿主細胞を感染させてVVpSX11L1L2が得られ 、宿主細胞のトランスフェクションの後に、これがし1およびL2を含有するV LPを生産する請求の範囲第1項記載の方法。 18.ワクシニア4bプロモーターに連結したHPV6bL1蛋白をコードする 遺伝子を含有する組換えDNA分子を構築し、引き続いて、これをプラスミドp SX3に挿入してプラスミドpSX36L1が得られ、しかる後、これで宿主細 胞を感染させてVV pSX36L1が得られ、宿主細胞のトランスフェクショ ンの後に、これがHPV6bL1を含有するVLPを生産する請求の範囲第1項 記載の方法。 19.ワクシニア28K後期プロモーターに連結したHPV6bL2をコードす る遺伝子を含有する組換えDNA分子を構築し、しかる後、これをpSX36L 1にクローンして二重組換えプラスミドpSX6L1L2が得られ、しかる後、 これで宿主細胞を感染させてVV pSX36L1L2が得られ、宿主細胞のト ランスフェクションの後に、これがHPV6bL1蛋白およびHPV6bL2蛋 白を含有するVLPを形成する請求の範囲第1項記載の方法。 20.請求の範囲第1項ないし第20項いずれか1項に記載のVLPとアジュバ ントを組み合わせる工程を包含するワクチンの製法。 21.請求の範囲第1項ないし第20項いずれか1項の方法で生産されたVLP 。 22.請求の範囲第21項の方法によって生産されたワクチン。 23.蛋白L1およびL2を含有するパピローマウイルスに由来するVLP。 24.蛋白L1およびL2を含有するHPV16に由来するVLP。 25.蛋白L1およびL2を含有するBPV1に由来するVLP。 26.蛋白L1を含有するBPV1に由来するVLP。 27.蛋白し1およびL2を含有するBPV1に由来するVLP。 28.蛋白L1を含有するHPV11に由来するVLP。 29.蛋白L1およびL2を含有するHPV6bに由来するVLP。 30.蛋白L1を含有するHPV6bに由来するVLP。 31.プラスミドPLC201。 32.パピローマウイルスキヤプシド蛋白L1およびL2のためのウイルスニ重 組換体。 33.パピローマウイルスキヤプシド蛋白L1のためのウイルス組換体。 34.プラスミドPLC200。 35.5′−【配列があります】−3′36.5′−【配列があります】−3′ 37.PLC201VV。 38.ポリクローナル抗−VLP HPV16抗血清。 39.図12に示したBエピトープ。 40.HPV6およびHPV11のし1およびL2蛋白のためのVV二重組換体 。 41. 5′−【配列があります】 42. 5′−【配列があります】 43. 5 【配列があります】 44. 5′−【配列があります】 45.pSXBPVL1。 46.P480BPVL2。 47.pSXBpVL1L2。 48.pSXBPVL1VV。 49.pSXBPVL1L2VV。 50.pSX11L1。 51.pSX11L1L2。 52.pSX6L1。 53.DSX6L1L2。 54.VV pSX11L1。 55.VV pSX11L1L2。 56.VV pSX6L1。 57.VV pSX6L1L2。 58. 5′【配列があります】 59. 5′【配列があります】 60. 5′【配列があります】 61. 5′【配列があります】 62. 5′【配列があります】 63. 5′【配列があります】 64.蛋白L1およびL2を含有するVLP粒子の検出工程を包含するエライサ 法によるパピローマウイルスの先行感染の診断方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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AUPK732291 | 1991-07-19 | ||
AU7322 | 1991-07-19 | ||
PCT/AU1992/000364 WO1993002184A1 (en) | 1991-07-19 | 1992-07-20 | Papilloma virus vaccine |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
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JP2003294384A Division JP2004000269A (ja) | 1991-07-19 | 2003-08-18 | パピローマウイルスワクチン |
JP2005339177A Division JP4255944B2 (ja) | 1991-07-19 | 2005-11-24 | パピローマウイルスワクチン |
Publications (2)
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JPH07505042A true JPH07505042A (ja) | 1995-06-08 |
JP3828570B2 JP3828570B2 (ja) | 2006-10-04 |
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Application Number | Title | Priority Date | Filing Date |
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JP50248693A Expired - Lifetime JP3828570B2 (ja) | 1991-07-19 | 1992-07-20 | パピローマウイルスワクチン |
JP2003294384A Withdrawn JP2004000269A (ja) | 1991-07-19 | 2003-08-18 | パピローマウイルスワクチン |
JP2005339177A Expired - Lifetime JP4255944B2 (ja) | 1991-07-19 | 2005-11-24 | パピローマウイルスワクチン |
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Application Number | Title | Priority Date | Filing Date |
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JP2003294384A Withdrawn JP2004000269A (ja) | 1991-07-19 | 2003-08-18 | パピローマウイルスワクチン |
JP2005339177A Expired - Lifetime JP4255944B2 (ja) | 1991-07-19 | 2005-11-24 | パピローマウイルスワクチン |
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EP (5) | EP1207203A3 (ja) |
JP (3) | JP3828570B2 (ja) |
KR (1) | KR100240480B1 (ja) |
AT (3) | ATE332973T1 (ja) |
AU (1) | AU651727B2 (ja) |
CA (1) | CA2113712C (ja) |
CY (5) | CY2598B2 (ja) |
DE (11) | DE122007000023I1 (ja) |
DK (3) | DK1359156T3 (ja) |
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HK (1) | HK1059271A1 (ja) |
LU (8) | LU91318I2 (ja) |
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JP2003508495A (ja) * | 1999-09-07 | 2003-03-04 | スミスクライン ビーチャム バイオロジカルズ ソシエテ アノニム | 新規組成物 |
JP4689910B2 (ja) * | 1999-09-07 | 2011-06-01 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | 新規組成物 |
JP2021126109A (ja) * | 2020-02-13 | 2021-09-02 | 国立研究開発法人農業・食品産業技術総合研究機構 | ウイルス様粒子及びその使用 |
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