JPH0746110B2 - Immunological measurement method - Google Patents
Immunological measurement methodInfo
- Publication number
- JPH0746110B2 JPH0746110B2 JP60113055A JP11305585A JPH0746110B2 JP H0746110 B2 JPH0746110 B2 JP H0746110B2 JP 60113055 A JP60113055 A JP 60113055A JP 11305585 A JP11305585 A JP 11305585A JP H0746110 B2 JPH0746110 B2 JP H0746110B2
- Authority
- JP
- Japan
- Prior art keywords
- light
- antibody
- measurement target
- measurement
- blood
- Prior art date
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- Expired - Lifetime
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Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、免疫学的反応に基づいて検体試料中の複数の
測定対象の存在を検出するための免疫学的測定方法に関
するものである。TECHNICAL FIELD The present invention relates to an immunological measurement method for detecting the presence of a plurality of measurement targets in a sample based on an immunological reaction.
検体試料中の種々の抗体または抗原の存在を検出するこ
とにより、検体の免疫学的な異常や型を判定する免疫学
的測定方法の一例として、本願人は特開昭56−2564号公
報において粒子凝集パターンを光電的に検出して血液型
判定を行なう血液型判定方法を提案している。この既知
の血液型判定方法では、分析すべき血液試料から遠心分
離により血球を分離し、分離した血球から２〜５％の血
球浮遊液を作り、この血球浮遊液を底部が円錐状をした
反応容器に分注すると共に特定の抗血清を加えて凝集さ
せる。そして、反応容器の底部に形成した凝集パターン
を一様に照明し、中心部から外側に向けて配置した３種
の受光素子により受光して、これら受光素子の出力信号
に基づいて凝集パターンを判定し、この結果に基づいて
血液型を判定している。As an example of an immunological measurement method for determining an immunological abnormality or type of a specimen by detecting the presence of various antibodies or antigens in a specimen sample, the present applicant discloses in JP-A-56-2564. A blood typing method has been proposed in which the particle aggregation pattern is photoelectrically detected to perform blood typing. In this known blood typing method, blood cells are separated from a blood sample to be analyzed by centrifugation, 2 to 5% of blood cell suspension is prepared from the separated blood cells, and the blood cell suspension has a conical bottom. Dispense into a container and add specific antiserum to cause aggregation. Then, the agglomeration pattern formed on the bottom of the reaction vessel is uniformly illuminated, and light is received by three types of light receiving elements arranged from the center to the outside, and the agglomeration pattern is determined based on the output signals of these light receiving elements. Then, the blood type is determined based on this result.
上述した既知の血液型判定方法では、判定すべき血液試
料を遠心分離によって分離し、次に生理食塩水を加えて
血球浮遊液とし、さらに反応容器内で特定の抗血清を加
えて反応させなければならず、凝集パターンを形成する
までに種々の前処理が必要であり、測定作業が面倒にな
る欠点があった。また、反応容器を長時間に亘って静置
して凝集パターンを形成しなければならず判定に長時間
かかる欠点もあった。更に、凝集パターンに基づいて判
別する方法では、凝集パターンを測定するために光源、
結像レンズ、複数個の受光素子等を正確に配置しなけれ
ばならず、判定装置が大型化且つ複雑化する欠点もあっ
た。In the known blood group determination method described above, the blood sample to be determined is separated by centrifugation, physiological saline is then added to form a hemocyte suspension, and a specific antiserum must be further added in the reaction container for reaction. In addition, various pretreatments are required until the aggregation pattern is formed, and there is a drawback that the measurement work is troublesome. In addition, the reaction container must be allowed to stand for a long time to form an aggregation pattern, which requires a long time for determination. Further, in the method of determining based on the aggregation pattern, a light source for measuring the aggregation pattern,
The image forming lens, a plurality of light receiving elements, and the like must be accurately arranged, and there is a drawback that the determination device becomes large and complicated.
従って、本発明の目的は上述した欠点を除去し、簡単か
つ小型の装置により異なる種類の測定対象を同時に測定
する免疫学的測定方法を提供するものである。Therefore, an object of the present invention is to eliminate the above-mentioned drawbacks and to provide an immunological measurement method for simultaneously measuring different kinds of measurement targets with a simple and compact device.
本発明による免疫学的測定方法は、検体試料中の異なる
種類の測定対象を測定する免疫学的測定方法において、
第１及び第２の端面を有し、側周に光遮断層が形成され
ている少なくとも２個の第１及び第２の光導体を用意
し、第１の光導体の第１の端面に第１の測定対象と特異
的に結合する抗原又は抗体を固相化し、第２の光導体の
第１の端面に第１の測定対象と異なる第２の測定対象と
特異的に結合する抗原又は抗体を固相化する工程と、 前記固相化された第１及び第２の光導体の第１の端面を
検体試料中に浸漬する工程と、 所定時間経過後に、第１及び第２の光導体の第１の端面
の光学的特性を、光導体の第１及び第２の端面を通る光
を用いて測定する工程と、 測定された光学的特性から各測定対象の存在を検出する
工程とを有することを特徴とするものである。The immunological measurement method according to the present invention is an immunological measurement method for measuring different types of measurement targets in a specimen sample,
At least two first and second light guides having first and second end faces and having a light-shielding layer formed on the side circumference thereof are prepared, and the first end face of the first light guide is provided with the first and second light guides. Antigen or antibody that specifically binds to one measurement target is immobilized, and an antigen or antibody that specifically binds to a second measurement target different from the first measurement target on the first end face of the second photoconductor Solid phase, a step of immersing the first end faces of the solid phased first and second light guides in a specimen sample, and after a predetermined time elapses, first and second light guides Measuring the optical characteristics of the first end surface of the light guide using light passing through the first and second end surfaces of the light guide, and detecting the presence of each measurement target from the measured optical characteristics. It is characterized by having.
さらに、本発明による免疫学的測定方法は、検体試料中
の異なる種類の測定対象を測定する免疫学的測定方法に
おいて、 第１及び第２の端面を有するロッド状レンズから成る１
本の光導体を用意し、第１の端面の異なる位置に互いに
異なる測定対象と特異的に結合する複数種類の抗原又は
抗体を固相化する工程と、 前記第１の端面を検体試料中に浸漬する工程と、 所定時間経過後に、前記第１の端面の光学像を第２の端
面を経て検出し、検出した光学像から検出すべき測定対
象の存在を検出する工程とを有することを特徴とするも
のである。Furthermore, the immunological measurement method according to the present invention is an immunological measurement method for measuring different types of measurement targets in a specimen sample, which comprises a rod-shaped lens having first and second end faces.
A step of preparing a plurality of light guides, and immobilizing a plurality of types of antigens or antibodies that specifically bind to different measurement targets at different positions on the first end surface; A step of immersing, and a step of detecting an optical image of the first end face via the second end face after a predetermined time has passed, and detecting the presence of a measurement target to be detected from the detected optical image. It is what
第１図は本発明による免疫学的測定方法を実施するため
の血液型判定装置の一例の構成を示す線図である。容器
１内に判定すべき全血状態の血液試料２を収容する。血
液試料２中にセンサ３の先端を浸漬する。センサ３は第
１、第２及び第３の３本の光導体4,5及び６から成り、
各端面3a,4a及び5aを一致させて一体的に結合して構成
する。３本の光導体４〜６は、例えば多数の光ファイバ
を束ねたファイババンドルで構成することができ、各光
導体４〜６の外周には光遮蔽層７を設けて外光の影響を
除去する。第１の光導体４の端面4aには血液試料中のＡ
型血球と特異的に光源抗体反応を行なう抗Ａ抗体９を固
相化し、第２の光導体４の端面4aには血液試料中のＢ型
血球と特異的に抗原抗体反応を行なう抗Ｂ抗体９を固相
化、第３の光導体６は基準用光導体とし、その端面6aに
は全く固相化しないものとする。照明光源10からミラー
11を介して各光導体４〜６の他方の端面を均一に照明す
る。また、第１〜第３の光導体４〜６の各光軸とそれぞ
れ対応するようにミラー11を介して第１、第２及び第３
の受光素子12,13及び14を配置する。照明光源10から発
した照明光はミラー11で反射し、第１〜第３の光導体４
〜６の他方の端面に均一に入射し、光導体の内部を伝搬
して各端面4a〜6aに到達する。第１及び第２の光導体５
及び６の端面4aおよび5aには抗Ａ抗体および抗Ｂ抗体が
それぞれ固相化されているから、判定すべき血液試料の
血液型に応じて各端面に血球が結合することになる。よ
って免疫反応によって端面に血球が結合した場合、照明
光源10から伝搬した照明光は血球表面で反射し、再び光
導体内を伝搬して第１及び第２の受光素子12及び13に入
射することになる。また、基準用光導体６の端面6aには
常に血球が結合しないため、第１及び第２の受光素子12
及び13の出力を第３の受光素子14の出力と比較すれば第
１及び第２の光導体の端面にＡ型又はＢ型の血球が結合
したか否かを容易に検出できることになる。FIG. 1 is a diagram showing the configuration of an example of a blood type determination device for carrying out the immunological measurement method according to the present invention. A blood sample 2 in a whole blood state to be judged is contained in a container 1. The tip of the sensor 3 is immersed in the blood sample 2. The sensor 3 consists of three first, second and third light guides 4, 5 and 6,
The respective end surfaces 3a, 4a and 5a are made to coincide with each other and integrally joined. The three optical conductors 4 to 6 can be configured by, for example, a fiber bundle in which a large number of optical fibers are bundled, and a light shielding layer 7 is provided on the outer periphery of each optical conductor 4 to 6 to remove the influence of external light. To do. On the end face 4a of the first light guide 4, A in the blood sample
Anti-A antibody 9 that specifically reacts with the blood cells of the light source is immobilized, and the end surface 4a of the second photoconductor 4 has the anti-B antibody that specifically reacts with the blood cells of B in the blood sample. It is assumed that 9 is a solid phase, the third light guide 6 is a reference light guide, and the end face 6a thereof is not solid phase at all. Mirror from illumination light source 10
The other end surface of each of the light guides 4 to 6 is uniformly illuminated via 11. In addition, the first, second and third mirrors 11 are provided so as to correspond to the respective optical axes of the first to third optical conductors 4 to 6, respectively.
The light receiving elements 12, 13 and 14 are arranged. The illumination light emitted from the illumination light source 10 is reflected by the mirror 11, and the first to third light guides 4
The light uniformly enters the other end surface of the optical waveguides 6 to 6, propagates inside the light guide, and reaches the end surfaces 4a to 6a. First and second light guide 5
Since the anti-A antibody and the anti-B antibody are immobilized on the end faces 4a and 5a of 6 and 6, blood cells will be bound to each end face depending on the blood type of the blood sample to be determined. Therefore, when blood cells are bound to the end surface due to an immune reaction, the illumination light propagating from the illumination light source 10 is reflected on the blood cell surface, propagates again in the light guide body, and is incident on the first and second light receiving elements 12 and 13. Become. In addition, since blood cells are not always bound to the end surface 6a of the reference light guide 6, the first and second light receiving elements 12
By comparing the outputs of 13 and 13 with the output of the third light receiving element 14, it is possible to easily detect whether or not A type or B type blood cells are bound to the end faces of the first and second light guides.
第２図は血液型判定回路の一例の構成を示す回路図であ
る。第１の光導体４からの反射光を受光する第１の受光
素子12と基準用の第３の光導体からの光を受光する第３
の受光素子14とを第１の差動増幅器15に接続し、第２の
光導体５からの反射光を受光する第２の受光素子13と基
準用の第３の受光素子14とを第２の差動増幅器16にそれ
ぞれ接続する。第１の差動増幅器15ではＡ型血球が結合
したか否かが検出され、第２の差動増幅器16ではＢ型の
血球が結合したか否かが検出される。これら第１及び第
２の差動増幅器15及び16の出力を信号処理回路17に供給
する。この信号処理回路17では表１に基づいて血液型を
判定する。FIG. 2 is a circuit diagram showing the configuration of an example of the blood type determination circuit. A first light receiving element 12 for receiving the reflected light from the first light guide 4 and a third for receiving the light from the third reference light guide.
The second light receiving element 13 for receiving the reflected light from the second optical conductor 5 and the third light receiving element 14 for reference are connected to the first differential amplifier 15 To the differential amplifier 16 of. The first differential amplifier 15 detects whether A-type blood cells are combined, and the second differential amplifier 16 detects whether B-type blood cells are combined. The outputs of the first and second differential amplifiers 15 and 16 are supplied to the signal processing circuit 17. The signal processing circuit 17 determines the blood type based on Table 1.
表 １ 第１光導体 第２光導体 血 液 型 ＋ − Ａ型 ＋ ＋ AB型 − ＋ Ｂ型 − − Ｏ型 ※＋は血球結合を表わし、−は血球が結合しない場合を
表わす。Table 1 1st light guide 2nd light guide Blood-liquid type + -A type + + AB type- + B type --- O type * + indicates blood cell binding, − indicates blood cell binding.
この信号処理回路17の判定結果をプリンタ等から成る表
示装置18に表示する。このように構成すれば、抗Ａ抗体
及び抗Ｂ抗体を端面に固相化した光導体と基準光導体と
から成る３本の光導体の端面を全血状態の血液試料中に
浸漬するだけで容易に血液型判定を行なうことができ
る。The determination result of the signal processing circuit 17 is displayed on the display device 18 such as a printer. According to this structure, the end surfaces of the three light guides, which are the light guide having the anti-A antibody and the anti-B antibody immobilized on the end surfaces and the reference light guide, are simply immersed in the blood sample in the whole blood state. Blood type can be easily determined.
第３図は血液試料中の抗Ａ抗体及び抗Ｂ抗体の有無に基
づいて血液型判定を行なういわゆる裏判定を行なうため
の装置の構成を示すものである。本例でも血液試料は全
血液状態の血液をそのまま用い、３本の光導体から成る
センサ20の先端を血液試料中に浸漬する。第１の光導体
21の端面には血液中の抗Ａ抗体と特異的に免疫反応をお
こすＡ抗原22を固相し、第２の光導体23の端面には血液
中の抗Ｂ抗体と特異的に免疫反応を起こすＢ抗原24を固
相化し、第３の光導体25の端面は固相化しないものとす
る。このように構成すれば、血液試料中に抗Ａ抗体又は
抗Ｂ抗体が含まれている場合、免疫反応によって、光導
体の端面が乳白色となるので、第１図に示すような方法
によって反射光を測定することによって血液試料中の抗
Ａ抗体及び抗Ｂ抗体が検出される。FIG. 3 shows the configuration of an apparatus for performing so-called backside determination for performing blood group determination based on the presence or absence of anti-A antibody and anti-B antibody in a blood sample. In this example as well, blood in the whole blood state is used as it is, and the tip of the sensor 20 composed of three light guides is immersed in the blood sample. First light guide
An A antigen 22 that specifically causes an immune reaction with the anti-A antibody in blood is solid-phased on the end face of 21, and an immune reaction with the anti-B antibody in blood is specifically performed on the end face of the second photoconductor 23. It is assumed that the B antigen 24 to be raised is solid-phased and the end face of the third photoconductor 25 is not solid-phased. According to this structure, when the blood sample contains the anti-A antibody or the anti-B antibody, the end face of the light guide becomes milky white due to the immune reaction. Therefore, the reflected light is reflected by the method shown in FIG. The anti-A antibody and anti-B antibody in the blood sample are detected by measuring
第４図は本発明による免疫学的測定方法を実施するため
の装置の変形例の構成を示す線図である。本例では血液
試料中のＡ抗原及びＢ抗原の有無に基づいて判定を行な
う所謂表判定と抗Ａ抗体及び抗Ｂ抗体の有無に基づいて
判定を行なう所謂裏判定を同時に行なう例を示す。セン
サ30は６本の光導体から成り、第1,第２及び第３の３本
の光導体31,32及び33の端面を一致させ、第4,第５及び
第６の３本の光導体34,35及び36の端面を一致させる。
そして、第１〜第３の光導体31〜33の端面を第４〜第６
の光導体34〜36の端面よりも突出するように設定する。
第１の光導体31の端面には抗Ａ抗体37を固相化し、第２
の光導体32の端面には抗Ｂ抗体38を固相し、第３の光導
体34は固相化せず基準用とする。また、第４の光導体34
の端面にはＡ抗原39を固相化し、第５の光導体35の端面
にはＢ抗原40を固相化し、第６の光導体36は基準用とす
る。本例では血液試料を静置して血球を容器底部に沈澱
させてから測定を行なう。第４図に示すように６本の光
導体31〜36の先端を血液試料中に浸漬する。第１〜第３
の光導体31,32及び33の各端面を血球中まで突出させ、
第４〜第６の光導体34,35及び36の各端面は上澄である
血漿中に位置させる。このように構成すれば、第１〜第
３の光導体31,32及び33によって血液試料中のＡ型又は
Ｂ型の血球の有無が検出されると共に、第４〜第６の光
導体34,35及び36によって血液試料中の抗Ａ抗体又は抗
Ｂ抗体の有無が同時に検出されることになる。このよう
に同一の血液試料について表判定及び裏判定を同時に行
なえば、判定精度を一層高めることができる。尚、本例
でも血球沈降前の全血状態の血液試料を用いても良好に
判定することができる。FIG. 4 is a diagram showing the configuration of a modified example of the apparatus for carrying out the immunological measurement method according to the present invention. This example shows an example in which so-called front-side determination, which makes a determination based on the presence or absence of A antigen and B antigen in a blood sample, and so-called back determination, which makes a determination based on the presence or absence of anti-A antibody and anti-B antibody, are performed simultaneously. The sensor 30 is composed of six light guides, and the end faces of the first, second and third light guides 31, 32 and 33 are aligned with each other, and the fourth, fifth and sixth light guides are arranged. Match the end faces of 34, 35 and 36.
Then, the end faces of the first to third light guides 31 to 33 are connected to the fourth to sixth ends.
It is set so as to protrude from the end faces of the light guides 34 to 36.
The anti-A antibody 37 is immobilized on the end face of the first light guide 31,
The anti-B antibody 38 is solid-phased on the end face of the light guide 32, and the third light guide 34 is not solidified and is used as a reference. Also, the fourth light guide 34
The A antigen 39 is solid-phased on the end face of, the B antigen 40 is solid-phased on the end face of the fifth photoconductor 35, and the sixth photoconductor 36 is used as a reference. In this example, the blood sample is allowed to stand and the blood cells are allowed to settle on the bottom of the container before measurement. As shown in FIG. 4, the tips of the six light guides 31 to 36 are immersed in a blood sample. First to third
Each end face of the light guides 31, 32 and 33 of is projected into the blood cell,
The end faces of the fourth to sixth light guides 34, 35 and 36 are located in the supernatant plasma. According to this structure, the presence or absence of A-type or B-type blood cells in the blood sample is detected by the first to third light guides 31, 32 and 33, and the fourth to sixth light guides 34, With 35 and 36, the presence or absence of anti-A antibody or anti-B antibody in the blood sample will be detected simultaneously. In this way, the determination accuracy can be further improved by simultaneously performing the front determination and the back determination on the same blood sample. It should be noted that in this example as well, good determination can be performed using a blood sample in a whole blood state before blood cell sedimentation.
第５図は本発明による免疫学的測定方法を実施するため
の装置の別の変形例の構成を示す斜視図である。本例で
は光導体として１本のロッド状のセルフォックレンズ50
を用い、セルフォックレンズ50の一方の端面に抗Ａ抗体
51、抗Ｂ抗体52、Ａ抗原53及びＢ抗原54それぞれ部分的
に固相化する。そして、セルフォックレンズ50の他方の
端面には４種の抗原又は抗体51〜54と対応する位置にCC
DやSIT等から成る４個の受光素子アレイ55〜58を装着
し、更に抗原及び抗体51〜54と対応しない位置に基準用
の受光素子アレイ59を装着する。このように構成すれ
ば、免疫反応によって抗Ａ抗体51、抗Ｂ抗体52、Ａ抗原
53及びＢ抗原54に結合した血液試料中の血球又は抗体の
像が他方の端面に結像され、その像が対応する位置に装
着した受光素子アレイ55〜58によって検出されることに
なる。従って、表判定および裏判定を同時に行なうこと
ができる。FIG. 5 is a perspective view showing the configuration of another modified example of the apparatus for carrying out the immunological measurement method according to the present invention. In this example, one rod-shaped SELFOC lens 50 is used as a light guide.
Using anti-A antibody on one end face of SELFOC lens 50
51, anti-B antibody 52, A antigen 53, and B antigen 54 are partially solid-phased. Then, on the other end surface of the SELFOC lens 50, CCs are provided at positions corresponding to the four kinds of antigens or antibodies 51 to 54.
Four light receiving element arrays 55 to 58 composed of D, SIT, etc. are mounted, and further, a reference light receiving element array 59 is mounted at a position that does not correspond to the antigens and antibodies 51 to 54. According to this structure, the anti-A antibody 51, the anti-B antibody 52, and the A antigen are reacted by the immune reaction.
An image of blood cells or antibodies in the blood sample bound to 53 and B antigen 54 is formed on the other end face, and the image is detected by the light receiving element arrays 55 to 58 mounted at corresponding positions. Therefore, the front side determination and the back side determination can be performed simultaneously.
本発明は上述した実施例だけに限定されるのではなく幾
多の変更や変形が可能である。例えば上述した実施例で
は抗原又は抗体を固相化した端面からの反射光に基づい
て血液中の抗体又は抗原の有無を判定する構成とした
が、照明用光源を、抗原または抗体を固相化した端面側
に配置し、結合した抗体又は抗原からの透過光に基づい
て抗原抗体反応の有無を判定できる。この場合ライトガ
イドを用いて光導体の端面を照明する構成とすれば、均
一に照明光を照射させることができる。The present invention is not limited to the above-described embodiments, but many modifications and variations are possible. For example, in the above-described embodiment, the presence or absence of the antibody or antigen in the blood is determined based on the reflected light from the end surface on which the antigen or antibody is immobilized. However, the illumination light source is immobilized on the antigen or antibody. The presence or absence of an antigen-antibody reaction can be determined based on the transmitted light from the bound antibody or antigen. In this case, if the light guide is used to illuminate the end face of the light guide, the illumination light can be uniformly emitted.
以上説明したように本発明によれば、１回の測定作業で
複数種類の測定対象を測定でき、この結果測定効率を一
層向上させることができる。さらに、血液型測定を行な
う場合、全血状態で測定でき種々の前処理が不要にな
る。As described above, according to the present invention, it is possible to measure a plurality of types of measurement objects with one measurement operation, and as a result, it is possible to further improve the measurement efficiency. Furthermore, when blood group measurement is performed, it can be measured in a whole blood state, and various pretreatments are unnecessary.
さらに、光導体をロッド状レンズで構成すれば、１本の
光導体を用いるだけで複数種類の測定対象を同時に測定
することができる。Further, if the light guide is constituted by a rod-shaped lens, it is possible to simultaneously measure a plurality of types of measurement targets by using only one light guide.
第１図は本発明による免疫学的測定方法を実施するため
の装置の一例の構成を示す線図、 第２図は判定回路の一例の構成を示す線図、 第３図、第４図及び第５図は本発明の免疫学的測定方法
を実施する血液型判定装置の変形例の構成を示す線図で
ある。 １……容器、２……血液試料 3,20,30……センサ 4,5,6,21,23,25,31……光導体 ７……遮蔽層、8,37,51……抗Ａ抗体 9,38,52……抗Ｂ抗体 10……照明光源、11……ミラー 12,13,14……受光素子 15,16……差動増幅器 17……信号処理回路、18……表示装置 22,39,53……Ａ抗原、24,40,54……Ｂ抗原 50……セルフォックレンズ 55,56,57,58,59……受光素子アレイFIG. 1 is a diagram showing a configuration of an example of an apparatus for carrying out an immunological measurement method according to the present invention, FIG. 2 is a diagram showing a configuration of an example of a determination circuit, FIGS. 3, 4, and FIG. 5 is a diagram showing the configuration of a modified example of the blood type determination apparatus for carrying out the immunological measurement method of the present invention. 1 ... Container, 2 ... Blood sample 3,20,30 ... Sensor 4,5,6,21,23,25,31 ... Optical conductor 7 ... Shielding layer, 8,37,51 ... Anti-A Antibody 9,38,52 ...... Anti-B antibody 10 ...... Illumination light source, 11 ...... Mirror 12,13,14 ...... Light receiving element 15,16 ...... Differential amplifier 17 ...... Signal processing circuit, 18 ...... Display device 22,39,53 …… A antigen, 24,40,54 …… B antigen 50 …… Selfoc lens 55,56,57,58,59 …… Light receiving element array
───────────────────────────────────────────────────── フロントページの続き (72)発明者 高橋 威夫 東京都渋谷区幡ヶ谷２丁目43番２号 オリ ンパス光学工業株式会社内 (56)参考文献 特表 昭56−501297（ＪＰ，Ａ) ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Takeo Takahashi 2-43-2 Hatagaya, Shibuya-ku, Tokyo Olympus Optical Co., Ltd. (56) References Special Table Sho 56-501297 (JP, A)
Claims (3)
する免疫学的測定方法において、第１及び第２の端面を
有し、側周に光遮断層が形成されている少なくとも２個
の第１及び第２の光導体を用意し、第１の光導体の第１
の端面に第１の測定対象と特異的に結合する抗原又は抗
体を固相化し、第２の光導体の第１の端面に第１の測定
対象と異なる第２の測定対象と特異的に結合する抗原又
は抗体を固相化する工程と、 前記固相化された第１及び第２の光導体の第１の端面を
検体試料中に浸漬する工程と、 所定時間経過後に、第１及び第２の光導体の第１の端面
の光学的特性を、光導体の第１及び第２の端面を通る光
を用いて測定する工程と、 測定された光学的特性から各測定対象の存在を検出する
工程とを有することを特徴とする免疫学的測定方法。1. An immunological measuring method for measuring different types of measuring objects in a specimen sample, comprising at least two light-shielding layers having first and second end faces and having a side circumference. First and second light guides are prepared, and the first light guide first
An antigen or an antibody that specifically binds to the first measurement target is immobilized on the end face of the second photoconductor, and a second measurement target that is different from the first measurement target is specifically bound to the first end face of the second photoconductor. Immobilizing the antigen or antibody to be immobilized, immersing the first end faces of the immobilized first and second photoconductors in a specimen sample, and after lapse of a predetermined time, the first and second Measuring the optical properties of the first end face of the second light guide using light passing through the first and second end faces of the light guide; and detecting the presence of each measurement object from the measured optical properties. An immunological measurement method comprising the step of:
対象とし、第２の測定対象と血球と関連する測定対象と
したことを特徴とする特許請求の範囲第１項に記載の免
疫学測定方法。2. The immunity according to claim 1, wherein the first measurement target is a serum-related measurement target, and the second measurement target is a blood cell-related measurement target. Measurement method.
する免疫学的測定方法において、 第１及び第２の端面を有するロッド状レンズから成る１
本の光導体を用意し、第１の端面の異なる位置に互いに
異なる測定対象と特異的に結合する複数種類の抗原又は
抗体を固相化する工程と、 前記第１の端面を検体試料中に浸漬する工程と、 所定時間経過後に、前記第１の端面の光学像を第２の端
面を経て検出し、検出した光学像から検出すべき測定対
象の存在を検出する工程とを有することを特徴とする免
疫学的測定方法。3. An immunological measuring method for measuring different kinds of measuring objects in a specimen sample, comprising a rod-shaped lens having first and second end faces.
A step of preparing a plurality of light guides, and immobilizing a plurality of types of antigens or antibodies that specifically bind to different measurement targets at different positions on the first end surface; A step of immersing, and a step of detecting an optical image of the first end surface through the second end surface after a lapse of a predetermined time, and detecting the presence of a measurement target to be detected from the detected optical image. And immunological measurement method.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60113055A JPH0746110B2 (en) | 1985-05-28 | 1985-05-28 | Immunological measurement method |
| DE19863617763 DE3617763A1 (en) | 1985-05-28 | 1986-05-27 | METHOD FOR CARRYING OUT IMMUNOLOGICAL PROVISIONS AND APPARATUS APPARATUS FOR THIS |
| US07/342,589 US4980278A (en) | 1985-05-28 | 1989-04-24 | Method of effecting immunological analysis and apparatus for carrying out the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60113055A JPH0746110B2 (en) | 1985-05-28 | 1985-05-28 | Immunological measurement method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61271459A JPS61271459A (en) | 1986-12-01 |
| JPH0746110B2 true JPH0746110B2 (en) | 1995-05-17 |
Family
ID=14602355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60113055A Expired - Lifetime JPH0746110B2 (en) | 1985-05-28 | 1985-05-28 | Immunological measurement method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0746110B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4738534A (en) * | 1987-03-26 | 1988-04-19 | Abbott Laboratories | Vertical beam spectrophotometer |
| JP2008503724A (en) * | 2004-06-17 | 2008-02-07 | バイエル・ヘルスケア・エルエルシー | Coaxial diffuse reflection read head |
| JP2016114357A (en) * | 2014-12-10 | 2016-06-23 | 学校法人日本大学 | Abo type blood back inspection device, and optical abo type blood back inspection method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4321057A (en) * | 1979-09-20 | 1982-03-23 | Buckles Richard G | Method for quantitative analysis using optical fibers |
| JPS589070A (en) * | 1981-04-29 | 1983-01-19 | チバ−ガイギ−・アクチエンゲゼルシヤフト | Device and kit for immunity analysis |
-
1985
- 1985-05-28 JP JP60113055A patent/JPH0746110B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61271459A (en) | 1986-12-01 |
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