JPH07285817A - Method for using special active water for plant - Google Patents
Method for using special active water for plantInfo
- Publication number
- JPH07285817A JPH07285817A JP6102200A JP10220094A JPH07285817A JP H07285817 A JPH07285817 A JP H07285817A JP 6102200 A JP6102200 A JP 6102200A JP 10220094 A JP10220094 A JP 10220094A JP H07285817 A JPH07285817 A JP H07285817A
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- Prior art keywords
- water
- stimulation
- plants
- methionine
- ethylene
- Prior art date
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Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、植物用特殊活性水の使
用方法に関するものであり、さらに詳しくは特定の植物
用特殊活性水に、天然のアミノ酸の一種であるメチオニ
ンを混用することによって、植物ホルモンであるエチレ
ン発生を促し、成長の促進と抑制、側枝の伸長促進、側
根の発根促進、開花の促進と抑制、呼吸作用の促進、タ
ンパク質合成の促進、耐病性の増大、果実の成熟促進等
の向上を促すことができる植物用特殊活性水の使用方法
に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for using special active water for plants, more specifically, by mixing methionine, which is one of natural amino acids, with special active water for plants. Promotes the production of ethylene, a plant hormone, promotes and suppresses growth, promotes lateral branch elongation, promotes lateral rooting, promotes and suppresses flowering, promotes respiratory action, promotes protein synthesis, increases disease resistance, and ripens fruit. The present invention relates to a method of using special activated water for plants, which can promote improvement such as promotion.
【０００２】[0002]
【従来の技術】従来農地では、エチレンの生理作用を利
用するために、煙霧、接触刺激、薬剤処理（例えばエチ
レン発生剤：商品名 エスレル）、エチレンガスの直接
処理などの方法がとられていたが、これらの方法は手間
や経験を有するものも多く、場合によっては上偏成長、
葉・花・果実の離脱促進、老化促進を引き起こし、植物
に対してマイナスとなることがあった。2. Description of the Related Art Conventionally, in agricultural fields, in order to utilize the physiological action of ethylene, methods such as fume, contact stimulus, chemical treatment (for example, ethylene generator: trade name Esrel), and direct treatment of ethylene gas have been adopted. However, many of these methods have troubles and experience, and in some cases upward growth,
It sometimes caused the leaves, flowers, and fruits to become detached and promote aging, which was negative for plants.
【０００３】[0003]
【発明が解決しようとする課題】本発明の目的は、植物
ホルモンであるエチレンの幅広い生理活性効果を増大
し、環境に対する悪影響がなく、しかも低コストで得ら
れるようにした植物用特殊活性水の使用方法を提供する
ことにある。The object of the present invention is to increase the wide range of physiologically active effects of ethylene, which is a plant hormone, so that it has no adverse effects on the environment and can be obtained at low cost, and it is a special active water for plants. To provide usage.
【０００４】[0004]
【課題を解決するための手段】本発明は植物用特殊活性
水の使用方法について鋭意研究した結果、植物用特殊活
性水にエチレンの前駆物質であるメチオニンを混用する
ことにより上記課題を解決することができることを見い
だし、本発明を成すに至った。Means for Solving the Problems As a result of intensive studies on the use of special active water for plants, the present invention solves the above problems by mixing methionine, which is a precursor of ethylene, with special active water for plants. The inventors have found that the above can be achieved and have completed the present invention.
【０００５】本発明の請求項１の発明は、純水もしくは
電解質溶液をアノード電解後、酵母菌の抽出液を添加し
て製造される植物用特殊活性水に、メチオニンを混用す
ることを特徴とする植物用特殊活性水の使用方法であ
る。The invention of claim 1 of the present invention is characterized in that methionine is mixed with specially activated water for plants produced by anodic electrolysis of pure water or an electrolyte solution and then adding an extract of yeast. It is a method of using special activated water for plants.
【０００６】本発明の請求項２の発明は、メチオニン濃
度を１０〜１００００ｐｐｍとすることを特徴とする請
求項１記載の植物用特殊活性水の使用方法である。The invention according to claim 2 of the present invention is the method for using special activated water for plants according to claim 1, characterized in that the methionine concentration is 10 to 10,000 ppm.
【０００７】本発明に使用するメチオニンは、天然に存
在するアミノ酸の一種で、植物体内で代謝されてエチレ
ンを生成する（図１）。メチオニンは石油化学の進歩に
より多量に生産されるので、二十余種類のアミノ酸の中
で最も原価の安いアミノ酸であり、家畜の飼料にも混入
されている。本発明において植物用特殊活性水中のメチ
オニン濃度は１０〜１００００ｐｐｍであるが、植物の
種類などによってエチレンの感受性が異なるので適宜選
定して決めることが好ましい。メチオニン濃度が１０ｐ
ｐｍ未満ではエチレン発生が少なく効果がない。メチオ
ニン濃度が１００００ｐｐｍを超えると水に溶解し難く
なり、エチレンが必要以上に発生して成長抑制、老化促
進などの害がでるので、好ましくは２０〜９０００ｐｐ
ｍ、より好ましくは５０〜５０００ｐｐｍ、特に好まし
くは１００〜１０００ｐｐｍとするのがよい。Methionine used in the present invention is one of naturally occurring amino acids and is metabolized in the plant body to produce ethylene (FIG. 1). Since methionine is produced in large quantities due to advances in petrochemistry, it is the cheapest amino acid of the 20 types of amino acids and is also mixed in livestock feed. In the present invention, the concentration of methionine in the special activated water for plants is 10 to 10,000 ppm, but the sensitivity of ethylene varies depending on the type of plant and the like, so it is preferable to appropriately select and determine. Methionine concentration is 10p
If it is less than pm, the amount of ethylene generated is small and there is no effect. When the methionine concentration exceeds 10,000 ppm, it becomes difficult to dissolve in water, and ethylene is generated more than necessary, which causes harmful effects such as growth inhibition and acceleration of aging. Therefore, it is preferably 20 to 9000 pp.
m, more preferably 50 to 5000 ppm, particularly preferably 100 to 1000 ppm.
【０００８】本発明では原料として水を用いるが活性酸
素種を長時間にわたり保持するためには純水であること
が好ましく、その電気伝導度は１０μＳ／ｃｍ以下、好
ましくは１μＳ／ｃｍ以下の高純水、さらに好ましくは
０．０６μＳ／ｃｍ以下の超純水を用いる。本発明では
電流密度を上げるためには原料水として電解質溶液を使
用することができる。In the present invention, water is used as a raw material, but is preferably pure water in order to retain active oxygen species for a long time, and its electric conductivity is 10 μS / cm or less, preferably 1 μS / cm or less. Further preferably, ultrapure water of 0.06 μS / cm or less is used. In the present invention, an electrolyte solution can be used as the raw material water in order to increase the current density.
【０００９】本発明において純水または電解質溶液を電
気分解するには方式は特に限定されず、一般に使用され
ている２槽式電解槽を用いることもできる。本発明にお
いて、３槽式電解槽は電解水の性質を広範囲に変化させ
ることができるので好ましく使用できる。In the present invention, the method for electrolyzing pure water or an electrolytic solution is not particularly limited, and a generally used two-tank type electrolytic tank can be used. In the present invention, the 3-tank electrolyzer can be preferably used because the property of electrolyzed water can be varied over a wide range.
【００１０】３槽式電解槽の断面構造の略図を図２に示
す。アノード槽およびカソード槽に純水８、中間槽２に
８．０×１０-4％（ｗ／ｖ）ＮａＣ１水溶液９を通水
し、電圧６Ｖ、流量：アノード槽１０００ｍｌ／ｍｉ
ｎ、中間槽５００ｍｌ／ｍｉｎ、カソード槽１０００ｍ
ｌ／ｍｉｎ、電極７は陰極、陽極いずれも白金を用いた
電解条件で電解して陽極に陽極水１０（酸性水、ｐＨ
２．７）を得る場合の電解条件の一例を表１に示す。１
はカソード槽、２は中間槽、３はアノード槽、４はイオ
ン交換槽、５はイオン交換膜、７は白金電極、８は純
水、１１は中性水、１２は陰極水（アルカリ水）を表
す。本発明における電気分解の条件は特に限定されない
が、電流密度は約５〜１５０ｍＡ／ｃｍ2 、陽極の材質
は白金、酸化ルテニユーム、β−酸化鉛などが好ましく
用いられる。陰極の材質は白金、カーボンなどが好まし
く用いられる。A schematic view of the cross-sectional structure of a three-cell type electrolytic cell is shown in FIG. Pure water 8 was passed through the anode tank and the cathode tank, and 8.0 × 10 −4 % (w / v) NaC1 aqueous solution 9 was passed through the intermediate tank 2, and the voltage was 6 V and the flow rate was 1000 ml / mi in the anode tank.
n, intermediate tank 500 ml / min, cathode tank 1000 m
1 / min, the electrode 7 is a cathode and the anode is electrolyzed under the electrolysis conditions using platinum and the anode water 10 (acid water, pH
Table 1 shows an example of electrolysis conditions for obtaining 2.7). 1
Is a cathode tank, 2 is an intermediate tank, 3 is an anode tank, 4 is an ion exchange tank, 5 is an ion exchange membrane, 7 is a platinum electrode, 8 is pure water, 11 is neutral water, and 12 is cathode water (alkaline water). Represents The conditions of electrolysis in the present invention are not particularly limited, but the current density is about 5 to 150 mA / cm 2 , and the material of the anode is preferably platinum, ruthenium oxide, β-lead oxide or the like. Platinum, carbon, etc. are preferably used as the material of the cathode.
【００１１】[0011]
【表１】 [Table 1]
【００１２】本発明においては植物用特殊活性水に市販
の微生物培養用酵母エキスや食品添加物用の酵母エキス
などを添加することができる。酵母エキスの添加量は植
物の種類などによっても異なり、適宜選定して決められ
る。In the present invention, commercially available yeast extract for culturing microorganisms, yeast extract for food additives and the like can be added to the special activated water for plants. The amount of yeast extract added varies depending on the type of plant and is appropriately selected and determined.
【００１３】本発明においては植物用特殊活性水は水で
希釈しないで使用することも可能である。通常は水で１
〜２０００倍に希釈して使用する。２０００倍を超えて
希釈すると効果が顕著でなくなり、また活性酸素種の安
定性が少なくなるので好ましくない。好ましくは１０〜
１５００倍、さらに好ましくは１００〜１０００倍、特
に好ましくは１５０〜８００倍に水で希釈して使用す
る。In the present invention, the special active water for plants can be used without being diluted with water. Usually 1 with water
Use it after diluting ~ 2000 times. If it is diluted more than 2000 times, the effect becomes unnoticeable and the stability of the active oxygen species decreases, which is not preferable. Preferably 10
It is diluted with water 1,500 times, more preferably 100 to 1,000 times, and particularly preferably 150 to 800 times.
【００１４】[0014]
【作用】本発明者は先に活性酸素群で処理した植物用特
殊活性水を提案した（特願平５−１２２２００号明細書
に記載のもの）。この植物用特殊活性水にエチレンの前
駆物質であるメチオニンを混用することにより、各々単
用で使用する場合に比してエチレン発生量が増大する。The present inventor has previously proposed special activated water for plants treated with an active oxygen group (described in Japanese Patent Application No. 5-122200). By mixing methionine, which is a precursor of ethylene, with this special activated water for plants, the amount of ethylene generated is increased as compared with the case where each is used alone.
【００１５】[0015]
【実施例】以下本発明を実施例により、具体的に説明す
るが、本発明はこれら実施例によって限定されるもので
はない。 （実施例１）供試品種は、タキイ種苗株式会社のトマト
の強力米寿２号を用い、１９９３年６月８日黒色３号ポ
リポット（直径９ｃｍ、高さ８ｃｍ）に播種した。ポリ
ポットには、腐葉土と赤土深土を１：２の割合で混合
し、石灰１．６ｇを加えた。赤土深土は風乾状態にし
て、１ｃｍ四方の篩を通して使用した。７月２７日に対
照区（水道水）、植物用特殊活性水５００倍稀釈区、メ
チオニン１０００ｐｐｍ区、および植物用特殊活性水５
００倍稀釈＋メチオニン１０００ｐｐｍ区に対し葉面散
布を行い、エチレン生成量を処理前、処理後１，２，
４，５日目に測定した。測定方法は葉面散布１時間後
に、上部展開第３葉（第５葉）を採取し、１ｃｍのコル
クボーラーで葉を打ち抜き、５０ｍｌの三角フラスコ内
に湿潤したろ紙を敷き、葉切片２０枚を入れた後、シリ
コン栓をし三角フラスコの全体を黒ビニールで巻いた。
さらにアルミホイルで覆い、２５℃で３時間インキュー
ベートし測定に供した。エチレン生成量については、ガ
スクロマトグラフ（島津製作所製ＧＣ−１４Ａ）を用
い、カラムはサンパックＡ、キャリヤガスはＮ2 （６ｋ
ｇ／ｃｍ2 ）、ディテクターはＦＩＤで１５０℃、イン
ジェクターを１２０℃、カラムは８０℃で各区３反復に
て測定を行い、平均値で表した。EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples. (Example 1) As a test variety, tomato strong rice longevity No. 2 of Takii Seed Co., Ltd. was used and seeded in a black No. 3 polypot (diameter 9 cm, height 8 cm) on June 8, 1993. In the polypot, mulch and red soil were mixed at a ratio of 1: 2, and 1.6 g of lime was added. The red soil deep soil was air-dried and passed through a 1 cm square sieve for use. On July 27, control area (tap water), special active water for plants diluted 500 times, methionine 1000 ppm area, and special active water for plants 5
Foliar spray was applied to 00 times dilution + methionine 1000 ppm, and the ethylene production was measured before and after treatment 1,2,
It was measured on the 4th and 5th days. The measurement method was as follows: 1 hour after spraying the leaves, the third leaf (the 5th leaf) developed above was punched, the leaf was punched out with a 1 cm cork borer, wet filter paper was laid in a 50 ml Erlenmeyer flask, and 20 leaf slices were placed. After putting it in, a silicon stopper was attached, and the entire Erlenmeyer flask was wrapped with black vinyl.
Further, it was covered with aluminum foil, incubated at 25 ° C. for 3 hours, and subjected to measurement. Regarding the ethylene production amount, a gas chromatograph (GC-14A manufactured by Shimadzu Corporation) was used, the column was Sunpack A, and the carrier gas was N 2 (6k).
g / cm 2 ), the detector was FID at 150 ° C., the injector was 120 ° C., and the column was 80 ° C. The measurement was repeated 3 times in each section, and the average value was shown.
【００１６】上記植物用特殊活性水は下記の方法により
調製したものを使用した。図２に記載の３槽式電解槽
（特願平５−１２２２００号明細書に記載のもの）を使
用し、アノード槽およびカソード槽に純水を通水し、中
間槽に８．０×１０-4％（ｗ／ｖ）ＮａＣ１水溶液を通
水した。表１に示した電解条件で電解して陽極に陽極水
（酸性水、ｐＨ２．７）を得た。The special activated water for plants used was prepared by the following method. Using the three-tank type electrolytic cell shown in FIG. 2 (described in Japanese Patent Application No. 5-122200), pure water was passed through the anode and cathode tanks, and the intermediate tank was 8.0 × 10. -4 % (w / v) NaCl aqueous solution was passed through. Electrolysis was performed under the electrolysis conditions shown in Table 1 to obtain anode water (acidic water, pH 2.7) in the anode.
【００１７】次に、以下に示すようにして酵母抽出エキ
スを得た。前培養として、培養液［ Yeast Nitrogen Ba
se (Difco)＋１％(W/v) Glucose］５０mlに、供試菌 Sa
ccharomyces cerevisiae 1FO-0234 1白金耳を接菌し
て、２７℃ overnight振とう培養した。この菌液を同上
の培養液９５０ｍｌに植菌し、同条件で本培養した。培
養後の菌液を遠心分離機にて２０００ｒｐｍ，５ｍｉｎ
遠沈した。上清除去後の沈殿物（菌体）に滅菌蒸留水を
加えよく懸濁し、同条件で遠沈した。この操作を３回繰
り返し沈殿物に培養液が残らないように洗浄した。湿菌
体（Wet weight :２０ｇ）を、５０％（ｖ／ｖ）エタノ
ール５０ｍｌに懸濁し、３０〜４０℃ overnight振とう
させながら自己融解させた。上記反応後の懸濁液は、孔
径０．４５μｍのメンブランフィルターにてろ過した。
ろ液はスプレードライし、酵母エキス粉末（成分組成を
表２に示す）を得た。Next, a yeast extract was obtained as shown below. As a pre-culture, the culture solution [Yeast Nitrogen Ba
se (Difco) + 1% (W / v) Glucose] 50 ml, the test strain Sa
ccharomyces cerevisiae 1FO-0234 1 platinum loop was inoculated and cultured at 27 ° C overnight with shaking. This bacterial solution was inoculated into 950 ml of the above-mentioned culture solution and main-cultured under the same conditions. Cultivated bacterial solution was centrifuged at 2000 rpm for 5 min
Spun off. Sterile distilled water was added to the precipitate (bacteria) after removal of the supernatant, and the suspension was well suspended and spun down under the same conditions. This operation was repeated 3 times, and the precipitate was washed so that the culture solution did not remain. Wet cells (Wet weight: 20 g) were suspended in 50 ml of 50% (v / v) ethanol and autolysed while shaking at 30-40 ° C overnight. The suspension after the reaction was filtered with a membrane filter having a pore size of 0.45 μm.
The filtrate was spray-dried to obtain yeast extract powder (component composition is shown in Table 2).
【００１８】[0018]
【表２】 [Table 2]
【００１９】前記の酸性水５００ｍｌに上記酵母エキス
粉末を５ｇ加え、溶解し、さらに孔径０．２０μｍのメ
ンブランフィルターにて滅菌濾過し、植物用特殊活性水
を作った。5 g of the above yeast extract powder was added to 500 ml of the above acidic water, dissolved, and sterilized with a membrane filter having a pore size of 0.20 μm to prepare special active water for plants.
【００２０】（試験結果）各区のエチレン生成量につい
て図３に結果を示した。図３に示すように、対照区に比
べ、対照区＜特殊活性水＜メチオニン＜特殊活性水＋メ
チオニンの順でエチレン生成量が増大した。特に特殊活
性水＋メチオニン処理区では処理区間におけるエチレン
生成量の増大が著しく、４日目には、対照区に比べ２．
８倍の増大を示した。一般に、エチレンは植物の地上部
の生育を抑制して根の生育を促進する。またｌｉｇｎｉ
ｆｉｃａｔｉｏｎを促進し、クチクラ（ｃｕｔｉｃｌ
ａ）蒸散を減少させて耐乾性を高めたり、菌の侵入を防
いで耐病性を高めたりすることが知られている。本試験
の結果は、特殊活性水＋メチオニンの葉面散布処理がト
マトの健苗育成に利用できることを示唆するものであ
る。(Test Results) The results of ethylene production in each section are shown in FIG. As shown in FIG. 3, compared with the control group, the ethylene production amount increased in the order of control group <special activated water <methionine <special activated water + methionine. Especially in the special activated water + methionine treatment group, the amount of ethylene produced in the treatment section increased remarkably.
It showed an 8-fold increase. In general, ethylene suppresses the growth of above-ground parts of plants and promotes the growth of roots. Also ligni
promote fication, cuticle
a) It is known that transpiration is reduced to improve drought resistance, or invasion of bacteria is prevented to improve disease resistance. The results of this test suggest that the foliar spray treatment of special activated water + methionine can be used for growing healthy tomato seedlings.
【００２１】[0021]
【発明の効果】本発明は植物用特殊活性水の使用方法に
関するものであり、環境に対する悪影響がなく、しかも
低コストで得られる特定の植物用特殊活性水に、天然の
アミノ酸の一種であるメチオニンを混用することによっ
て、植物ホルモンであるエチレン発生を促進し、成長の
促進と抑制、側枝の伸長促進、側根の発根促進、開花の
促進と抑制、呼吸作用の促進、タンパク質合成の促進、
耐病性の増大、果実の成熟促進等の向上を促すことがで
きる。INDUSTRIAL APPLICABILITY The present invention relates to a method for using special activated water for plants, which has no adverse effects on the environment and can be obtained at a low cost in specific activated water for plants, which is a kind of natural amino acid methionine. By promoting the production of ethylene, which is a plant hormone, by promoting the growth and suppression of growth, the promotion of lateral branch elongation, the promotion of lateral rooting, the promotion and suppression of flowering, the promotion of respiratory action, the promotion of protein synthesis,
It is possible to promote the improvement of disease resistance, the promotion of fruit ripening and the like.
【図１】 メチオニンからエチレンの生成される代謝経
路を示す説明図である。FIG. 1 is an explanatory diagram showing a metabolic pathway in which ethylene is produced from methionine.
【図２】 ３槽電解槽の断面を示す説明図である。FIG. 2 is an explanatory view showing a cross section of a three-cell electrolytic cell.
【図３】 エチレン生成量と処理後日数の関係を示すグ
ラフである。FIG. 3 is a graph showing the relationship between the amount of ethylene produced and the number of days after treatment.
１ カソード槽 ２ 中間槽 ３ アノード槽 ４ イオン交換樹脂 ５ イオン交換膜 ７ 白金電極 ８ 純水 ９ 食塩水 １０ 陽極水 １１ 中性水 １２ 陰極水 1 Cathode tank 2 Intermediate tank 3 Anode tank 4 Ion exchange resin 5 Ion exchange membrane 7 Platinum electrode 8 Pure water 9 Salt water 10 Anode water 11 Neutral water 12 Cathode water
フロントページの続き (72)発明者 河合 博 神奈川県横浜市戸塚区戸塚町4529−１Continued Front Page (72) Inventor Hiroshi Kawai 4529-1 Totsuka-cho, Totsuka-ku, Yokohama-shi, Kanagawa
Claims (2)
後、酵母菌の抽出液を添加して製造される植物用特殊活
性水に、メチオニンを混用することを特徴とする植物用
特殊活性水の使用方法。1. Use of special active water for plants, characterized in that methionine is mixed with special active water for plants produced by adding electrolysis solution of yeast after anodic electrolysis of pure water or electrolyte solution. Method.
ｍとすることを特徴とする請求項１記載の植物用特殊活
性水の使用方法。2. A methionine concentration of 10 to 10,000 pp
The method for using special activated water for plants according to claim 1, wherein m is m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10220094A JP3642434B2 (en) | 1994-04-15 | 1994-04-15 | How to use special active water for plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10220094A JP3642434B2 (en) | 1994-04-15 | 1994-04-15 | How to use special active water for plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH07285817A true JPH07285817A (en) | 1995-10-31 |
| JP3642434B2 JP3642434B2 (en) | 2005-04-27 |
Family
ID=14321026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10220094A Expired - Fee Related JP3642434B2 (en) | 1994-04-15 | 1994-04-15 | How to use special active water for plants |
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| Country | Link |
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| JP (1) | JP3642434B2 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997041732A1 (en) * | 1996-05-07 | 1997-11-13 | Hiroshi Kawai | Effervescent preparation for plants |
| WO1998051155A1 (en) * | 1997-05-09 | 1998-11-19 | Hiroshi Kawai | Method of preventing plant diseases and composition therefor |
| WO1999012409A1 (en) * | 1997-09-08 | 1999-03-18 | Hiroshi Kawai | Composition for improving dietary nutritive component of plant and method for improving dietary nutritive component of plant |
| WO2002049969A3 (en) * | 2000-12-21 | 2003-01-30 | Fraunhofer Ges Forschung | Method for accelerating biocatalytic and/or hormonal processes and use of the same |
| JP2003073210A (en) * | 2001-09-03 | 2003-03-12 | Daiwa Kasei Kk | Composition for promoting growth of plant and method for the same |
| JP2003095821A (en) * | 2001-09-25 | 2003-04-03 | Kao Corp | Plant vitality-increasing agent |
| JP2014080398A (en) * | 2012-10-18 | 2014-05-08 | Yukijirushi Shubyo Kk | Plant growth regulator |
-
1994
- 1994-04-15 JP JP10220094A patent/JP3642434B2/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997041732A1 (en) * | 1996-05-07 | 1997-11-13 | Hiroshi Kawai | Effervescent preparation for plants |
| WO1998051155A1 (en) * | 1997-05-09 | 1998-11-19 | Hiroshi Kawai | Method of preventing plant diseases and composition therefor |
| WO1999012409A1 (en) * | 1997-09-08 | 1999-03-18 | Hiroshi Kawai | Composition for improving dietary nutritive component of plant and method for improving dietary nutritive component of plant |
| WO2002049969A3 (en) * | 2000-12-21 | 2003-01-30 | Fraunhofer Ges Forschung | Method for accelerating biocatalytic and/or hormonal processes and use of the same |
| JP2003073210A (en) * | 2001-09-03 | 2003-03-12 | Daiwa Kasei Kk | Composition for promoting growth of plant and method for the same |
| JP2003095821A (en) * | 2001-09-25 | 2003-04-03 | Kao Corp | Plant vitality-increasing agent |
| JP2014080398A (en) * | 2012-10-18 | 2014-05-08 | Yukijirushi Shubyo Kk | Plant growth regulator |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3642434B2 (en) | 2005-04-27 |
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