JPH06305921A - Plant growth regulator treated with active oxygen group and production and use thereof - Google Patents
Plant growth regulator treated with active oxygen group and production and use thereofInfo
- Publication number
- JPH06305921A JPH06305921A JP5122200A JP12220093A JPH06305921A JP H06305921 A JPH06305921 A JP H06305921A JP 5122200 A JP5122200 A JP 5122200A JP 12220093 A JP12220093 A JP 12220093A JP H06305921 A JPH06305921 A JP H06305921A
- Authority
- JP
- Japan
- Prior art keywords
- plant
- plant growth
- growth regulator
- oxygen
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、植物生育調節剤、その
製造方法及びその使用方法に関するものであり、詳しく
は植物体からの植物ホルモンであるエチレン、一般にフ
ィトンチッド（フィトアレキシン・ジャスモン酸）と呼
ばれる揮発性物質の発生を調節し、さらに植物中の酵素
を活性化することによる成長促進作用、また特定酵素に
選択的に働く事による成長制御作用、酵素阻害物質によ
る成長抑制作用、酸素失活の防止作用、阻害物質の除去
ないし阻害物質の解離または置換などによる成長促進作
用など、植物の生理活性機構の向上を促すことが可能な
植物生育調節剤、その製造方法及び使用方法に関するも
のである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plant growth regulator, a method for producing the same, and a method for using the same. More specifically, ethylene, which is a plant hormone from a plant, generally phytoncide (phytoalexin jasmonic acid). It regulates the generation of volatile substances called so-called "growth-promoting effects" by activating enzymes in plants, growth-controlling effects by selectively acting on specific enzymes, growth-suppressing effects by enzyme inhibitors, and oxygen loss. The present invention relates to a plant growth regulator capable of promoting the improvement of the physiological activity mechanism of plants, such as the activity-preventing action, the growth-promoting action by removing the inhibitor or dissociating or substituting the inhibitor, and the production method and use thereof. is there.
【０００２】[0002]
【従来の技術】従来農地では、病害・害虫に対し様々な
薬剤が使用されてきた。最近、農薬の低毒化と毒性に対
する理解度が向上してきてはいるが、平成元年度の生産
量でみると、劇物３３．３％、毒物１％と取扱いにかな
りの注意を要するものも多く、農薬使用にともなう事故
の発生は相変わらず続いている。そのなかでも殺菌剤、
殺虫剤の占める割合は大変大きく、問題となっている。2. Description of the Related Art Conventionally, various chemicals have been used for diseases and pests in farmland. Recently, the understanding of pesticide detoxication and toxicity has been improving, but in terms of the production volume in the 1989 fiscal year, there are 33.3% of deleterious substances and 1% of poisonous substances, and some require considerable attention in handling. In many cases, accidents associated with the use of pesticides continue to occur. Among them, germicide,
The proportion of insecticides is very large, which is a problem.
【０００３】またそれらの薬剤を使用することで、本来
植物が持っている抗菌性物質［エチレン、エチレンオキ
サイド、クチン、俗に緑の香りと呼ばれる青葉アルコー
ル（ｃｉｓ−３−ヘキセン−１−オール）、フィトアレ
キシン］を調整させる能力を低下させ、植物自体の免疫
力をなくしてしまっているだけではなく、成長が著しく
阻害されていた。Further, by using these agents, antibacterial substances originally possessed by plants [ethylene, ethylene oxide, cutin, green leaf alcohol (cis-3-hexen-1-ol) commonly known as a green scent. , Phytoalexin] was not regulated, and not only the immunity of the plant itself was lost, but also the growth was significantly inhibited.
【０００４】さらに農地では、植物の生育促進のため、
植物内の反応速度や平衡を変化させて酵素活性を高める
目的で、カルシウム等の硅酸系強アルカリ酸化物を配合
している化学肥料を使用している場合が多く、その強ア
ルカリの性質上農作物を枯らしてしまう、土壌の安定に
必要な土壌菌を死滅させるなど、金属イオンによる生体
内酵素賦活作用よりもその欠点が優先する事があった。Further, in agricultural land, to promote the growth of plants,
Chemical fertilizers containing silicate-based strong alkali oxides such as calcium are often used for the purpose of changing the reaction rate and equilibrium in the plant to enhance enzyme activity. There have been cases where the drawbacks have priority over the in vivo enzyme activation effect of metal ions, such as death of agricultural crops and death of soil fungi necessary for soil stability.
【０００５】また植物性及び動物性の堆肥を利用する場
合も、その作用の多面性によって一定の方向性がないた
めに、ある作用は有益であっても別の作用は有害な場合
もあり、実用的には用途が限定されていた。In the case of utilizing plant and animal manures, one effect may be beneficial but another effect may be harmful because there is no specific direction due to the multifaceted nature of the effect. The use was limited in practice.
【０００６】それ以外にも植物の伸長成長、成長抑制、
肥大成長、細胞***、気孔の開閉、花芽形成、屈性、老
化、発芽、発根などの生理活性を促す植物ホルモンを利
用するものに、動植物中に広く分布するオーキシン、サ
イトカイニン、高等植物とカビに主として分布するジベ
レリン、高等植物に広く分布するアブシジン酸、エチレ
ン、ステロイド型のブラシノライド等があるが、その作
用はある種の特異性と多面性とが共存し、系全体として
の生理活性を促すには選択性に乏しく、また何よりも高
価であった。[0006] In addition to the above, plant growth, growth suppression,
It uses plant hormones that promote physiological activities such as hypertrophic growth, cell division, stomatal opening, flower bud formation, tropism, senescence, germination and rooting. , Abscisic acid, ethylene, and steroidal brassinolide, which are widely distributed in higher plants, but their actions coexist with certain specificity and pleiotropic properties, and the physiological activity of the whole system It was poorly selective, and above all expensive.
【０００７】[0007]
【発明が解決しようとする課題】本発明の目的は、植物
体からの植物ホルモンであるエチレン、一般にフィトン
チッドと呼ばれる揮発性物質の発生を調節すると同時
に、植物中の酵素を活性化することによる成長促進作
用、また特定の酵素に選択的に働く事による成長制御作
用、酵素阻害物質による成長抑制作用、酵素失活の防止
作用、阻害物質の除去ないし阻害物質の解離または置換
などによる成長促進作用など、植物の生理活性機構の向
上を促すことができる植物成長調節剤、その製造方法及
びその使用方法を提供することである。The object of the present invention is to regulate the generation of ethylene, a phytohormone, a volatile substance generally called phytoncide, from a plant, and at the same time, to grow by activating an enzyme in the plant. Promoting action, growth controlling action by selectively acting on a specific enzyme, growth inhibiting action by enzyme inhibitor, enzyme deactivating preventing action, growth promoting action by removing inhibitor or dissociating or replacing inhibitor Another object of the present invention is to provide a plant growth regulator capable of promoting improvement of physiological activity mechanism of plants, a method for producing the same, and a method for using the same.
【０００８】[0008]
【課題を解決するための手段】本発明者らは、これまで
知られていた各種の植物生理活性物質を、活性酸素群で
処理することによりその活性を高め、さらに不飽和有機
化合物の過酸化物を与えてやることにより、植物体自身
が抗菌性物質を発生し、また植物生育促進物質と活性酸
素群のバランスを変えてやることにより植物成長を調節
できることを見いだし、本発明を成すに到った。[Means for Solving the Problems] The present inventors increased the activity of various known plant physiologically active substances by treating them with an active oxygen group, and further peroxidated unsaturated organic compounds. It was found that the plant itself generates an antibacterial substance by giving a substance, and that the plant growth can be regulated by changing the balance between the plant growth promoting substance and the active oxygen group, and the present invention has been completed. It was.
【０００９】本発明の請求項１の発明は、不飽和脂肪酸
とそのエステル化合物、およびその重合物、不飽和脂肪
酸を含む微生物抽出液または植物抽出液、有機質肥料、
有害物質を除いた活性汚泥、ビタミン、ユビキノンから
なる群から選択される少なくとも一種と、 3Ｏ2 の一電
子還元種であるスーパーオキシド（Ｏ2 -）、励起状態の
酸素分子である励起三重項酸素（ 3Ｏ2 *）、一重項酸素
（ 1Ｏ2 ）、ヒドロキシラジカル（・ＯＨ）、オゾン
（Ｏ3 ）、金属オキソ種を含む金属−酸素錯体、過酸化
水素（Ｈ2 Ｏ2 ）、次亜塩素酸、亜塩素酸、塩素酸、過
塩素酸、ペルオキシラジカル（ＬＯＯ・）、アルコキシ
ラジカル（ＬＯ・）、ヒドロペルオキシド（ＬＯＯＨ）
から選択される少なくとも一種の活性酸素群との反応で
生成した、過酸化物を含む植物生育調節剤である。The invention of claim 1 of the present invention is an unsaturated fatty acid and its ester compound, and its polymer, a microorganism extract or plant extract containing unsaturated fatty acid, an organic fertilizer,
Activated sludge excluding harmful substances, and at least one vitamin is selected from the group consisting of ubiquinone, 3 O 2 superoxide which is an electron reduced species (O 2 -), triplet oxygen molecules in the excited state Oxygen ( 3 O 2 * ), singlet oxygen ( 1 O 2 ), hydroxy radical (.OH), ozone (O 3 ), metal-oxygen complex containing metal oxo species, hydrogen peroxide (H 2 O 2 ), Hypochlorous acid, chlorous acid, chloric acid, perchloric acid, peroxy radical (LOO.), Alkoxy radical (LO.), Hydroperoxide (LOOH)
It is a plant growth regulator containing peroxide, which is produced by a reaction with at least one active oxygen group selected from the group consisting of:
【００１０】本発明の請求項２の発明は、電解処理、超
音波処理、紫外線照射処理、化学的処理（薬剤添加）か
らなる群から選ばれる少なくとも一種の方法で純水また
は電解質溶液を処理して請求項１記載の活性酸素群を発
生させ、不飽和脂肪酸とそのエステル化合物、およびそ
の重合物、不飽和脂肪酸を含む微生物抽出液または植物
抽出液、有機質肥料、有害物質を除いた活性汚泥、ビタ
ミン、ユビキノンからなる群から選択される少なくとも
一種と反応させることを特徴とする植物生育調節剤の製
造方法である。According to a second aspect of the present invention, pure water or an electrolytic solution is treated by at least one method selected from the group consisting of electrolytic treatment, ultrasonic treatment, ultraviolet irradiation treatment, and chemical treatment (chemical addition). An unsaturated fatty acid and its ester compound, and a polymerized product thereof, a microorganism extract or plant extract containing unsaturated fatty acid, organic fertilizer, activated sludge from which harmful substances are removed, by generating the active oxygen group according to claim 1. A method for producing a plant growth regulator, which comprises reacting with at least one selected from the group consisting of vitamins and ubiquinone.
【００１１】本発明の請求項３の発明は、３槽式電解槽
を使用して、純水または電解質溶液を電解することによ
り活性酸素群を発生させることを特徴とする請求項２記
載の植物生育調節剤の製造方法。The invention of claim 3 of the present invention is characterized in that the active oxygen group is generated by electrolyzing pure water or an electrolyte solution using a three-cell type electrolytic cell. Method for producing growth regulator.
【００１２】本発明の請求項４の発明は、さらに熱処理
することを特徴とする請求項２あるいは請求項３記載の
植物生育調節剤の製造方法。The invention according to claim 4 of the present invention further comprises heat treatment, which is a method for producing a plant growth regulator according to claim 2 or claim 3.
【００１３】本発明の請求項５の発明は、さらにｐＨ調
整してｐＨ５〜７にすることを特徴とする請求項２ない
し請求項４記載の植物生育調製剤の製造方法である。The invention according to claim 5 of the present invention is the method for producing a plant growth regulator according to any one of claims 2 to 4, characterized in that the pH is further adjusted to pH 5 to 7.
【００１４】本発明の請求項６の発明は、請求項１記載
の植物生育調節剤を、１〜２０００倍に水で希釈して植
物に適用することを特徴とする植物生育調節剤の使用方
法である。The invention of claim 6 of the present invention is a method of using the plant growth regulator, which comprises diluting the plant growth regulator of claim 1 with water 1 to 2000 times and applying it to plants. Is.
【００１５】本発明の不飽和脂肪酸とそのエステル化合
物、およびその重合物とは、二重結合にはさまれた活性
メチレン基を有する脂肪酸とそのエステル化合物であ
り、例えばリノール酸、リノレン酸などを例示すること
ができる。The unsaturated fatty acid, the ester compound thereof, and the polymer thereof of the present invention are a fatty acid having an active methylene group sandwiched by a double bond and its ester compound, such as linoleic acid and linolenic acid. It can be illustrated.
【００１６】本発明の不飽和脂肪酸を含む微生物抽出液
または植物抽出液とは、微生物または植物の酸、アルカ
リ、アルコールあるいはエーテルなどによる抽出液であ
り、例えばイーストエキスなどを例示することができ
る。The microbial extract or plant extract containing unsaturated fatty acid of the present invention is an extract of microorganisms or plants with acid, alkali, alcohol or ether, and examples thereof include yeast extract.
【００１７】本発明の有機質肥料とは、堆肥（わら堆
肥、バーク堆肥など）、牛糞尿、鶏糞、緑肥などの通常
用いられているものでよく、特に限定されるものではな
い。The organic fertilizer of the present invention may be a commonly used one such as compost (straw compost, bark compost, etc.), cow manure, chicken manure, green manure, etc., and is not particularly limited.
【００１８】本発明で用いる有害物質を除いた活性汚泥
とは、人ぷん尿などの活性汚泥処理に用いられた活性汚
泥から重金属などの有害物質を除いたもので、通常は廃
棄物となるものであり、具体的な例としては活性汚泥肥
料［三菱油化（株）製］、人ぷん尿処理物（多摩川衛生
組合製）などの汚泥肥料や、乾燥菌体肥料などを挙げる
ことができる。The activated sludge excluding harmful substances used in the present invention is an activated sludge used for the treatment of activated sludge such as human excrement from which harmful substances such as heavy metals have been removed, and usually becomes waste. As specific examples, activated sludge fertilizers (manufactured by Mitsubishi Yuka Co., Ltd.), sludge fertilizers such as human waste treated products (manufactured by Tama River Sanitation Co., Ltd.), and dry cell fertilizers can be mentioned.
【００１９】本発明のビタミンとは、脂溶性ビタミン
（ビタミンＡ、Ｄ、Ｅ、Ｋなど）や水溶性ビタミン（ビ
タミンＢ群、ビタミンＰ、ビタミンＵ、コリン、ナイア
シン、パントテン酸、ビオチン、葉酸、ＰＱＱ、リポ
酸、イノシトール、ビタミンＣなど）である。The vitamins of the present invention include fat-soluble vitamins (vitamins A, D, E, K, etc.) and water-soluble vitamins (vitamin B group, vitamin P, vitamin U, choline, niacin, pantothenic acid, biotin, folic acid, PQQ, lipoic acid, inositol, vitamin C, etc.).
【００２０】本発明のユビキノンとは、２，３−ジメト
キシ−５−メチル−６−ポリプレニル−１，４−ベンゾ
キノンの総称であり、例えば、オウランチオグリオクラ
デイン、フミガチン、プラストキノンＡ、ロードキノ
ン、ユビクロメノール−ｎなどを例示することができ
る。The ubiquinone of the present invention is a general term for 2,3-dimethoxy-5-methyl-6-polyprenyl-1,4-benzoquinone, and includes, for example, auranthiogliocladein, fumigatine, plastoquinone A, and rhodoquinone. , Ubichromenol-n and the like.
【００２１】本発明のおいては、上記の群から選択され
る一種あるいは二種以上を組み合わせて使用する。組み
合わせの種類、配合比などは任意であり、適宜使用目的
などに応じて選定して決めることができる。In the present invention, one kind or a combination of two or more kinds selected from the above group is used. The type of combination, the compounding ratio, etc. are arbitrary and can be appropriately selected and determined according to the purpose of use.
【００２２】本発明の活性酸素群は広義の活性酸素群で
あり、 3Ｏ2 の一電子還元種であるスーパーオキシド
（Ｏ2 -）、励起状態の酸素分子である励起三重項酸素
（ 3Ｏ2 *）、一重項酸素（ 1Ｏ2 ）、ヒドロキシラジカ
ル（・ＯＨ）、オゾン（Ｏ3 ）、金属オキソ種を含む金
属−酸素錯体、過酸化水素（Ｈ2 Ｏ2 ）、次亜塩素酸、
亜塩素酸、塩素酸、過塩素酸、ペルオキシラジカル（Ｌ
ＯＯ・）、アルコキシラジカル（ＬＯ・）、ヒドロペル
オキシド（ＬＯＯＨ）の群から選択される一種あるいは
二種以上を組み合わせて使用することができる。組み合
わせの種類、配合比などは任意であり、適宜使用目的な
どに応じて選定して決めることができる。The active oxygen group of the present invention is a broad active oxygen group, superoxide an electron reduced species of 3 O 2 (O 2 -) , oxygen molecules in the excited state triplet oxygen (3 O 2 * ), singlet oxygen ( 1 O 2 ), hydroxy radical (.OH), ozone (O 3 ), metal-oxygen complex containing metal oxo species, hydrogen peroxide (H 2 O 2 ), hypochlorous acid ,
Chlorous acid, chloric acid, perchloric acid, peroxy radical (L
One or two or more selected from the group consisting of OO.), Alkoxy radicals (LO.) And hydroperoxide (LOOH) can be used in combination. The type of combination, the compounding ratio, etc. are arbitrary and can be appropriately selected and determined according to the purpose of use.
【００２３】本発明において両者を反応して本発明の植
物生育調節剤を生成する反応条件は特に限定されず、適
宜使用目的などに応じて選定して決めることができる
が、電解処理、超音波処理、紫外線照射処理、化学的処
理（薬剤添加）などの方法で純水または電解質溶液を処
理して上記の活性酸素群を発生させ、不飽和脂肪酸とそ
のエステル化合物、およびその重合物、不飽和脂肪酸を
含む微生物抽出液または植物抽出液、有機質肥料、有害
物質を除いた活性汚泥、ビタミン、ユビキノンなどと反
応させて植物生育調節剤を製造することが好ましい。反
応は好ましくは二価鉄などの触媒の存在下に行うことが
よい。In the present invention, the reaction conditions for reacting the two to produce the plant growth regulator of the present invention are not particularly limited and can be appropriately selected and determined according to the purpose of use, but electrolytic treatment, ultrasonic wave Treatment of pure water or electrolyte solution with a method such as treatment, UV irradiation treatment, chemical treatment (chemical addition), etc. to generate the above-mentioned active oxygen group, unsaturated fatty acid and its ester compound, and its polymer, unsaturated It is preferable to produce a plant growth regulator by reacting with a microbial extract or plant extract containing fatty acid, organic fertilizer, activated sludge from which harmful substances are removed, vitamins, ubiquinone and the like. The reaction is preferably carried out in the presence of a catalyst such as ferric iron.
【００２４】本発明の植物生育調節剤は、上記の反応に
よって生成するものであり、その化学的成分は有機物と
しては、１１−ホルミルウンデセン酸などのカルボン
酸、ヘキセノールなどのアルコール、ヘキセナールなど
のアルデヒドなど、鉄−酸素錯体、ポルフィリン錯体な
どの錯化合物、無機物としてはＮ、Ｐ、Ｋなどがあり、
有機物、無機物、錯化合物を含む酸性あるいはアルカリ
性水性溶液である。これらの含有量は原料の種類、量、
反応条件などの要因により異なり、限定することはでき
ないが、使用目的によりこれらの要因を制御することに
より一定の品質の生成物を得ることができる。これらの
化学的成分はそれぞれの効果を発揮するとともに、互い
の相乗効果により、植物体からの植物ホルモンの発生を
調節すると同時に、植物中の酵素を活性化することによ
る成長促進作用、また特定の酵素に選択的に働く事によ
る成長制御作用、酵素阻害物質による成長抑制作用、酵
素失活の防止作用、阻害物質の除去ないし阻害物質の解
離または置換などによる成長促進作用など、植物の生理
活性機構の向上を促す。The plant growth regulator of the present invention is produced by the above reaction, and its chemical components include organic substances such as carboxylic acids such as 11-formylundecenoic acid, alcohols such as hexenol, and hexenal. Aldehydes, complex compounds such as iron-oxygen complexes, porphyrin complexes, and inorganic substances include N, P, and K.
An acidic or alkaline aqueous solution containing an organic substance, an inorganic substance and a complex compound. The contents of these are the types of raw materials, the amount,
Although it depends on factors such as reaction conditions and cannot be limited, it is possible to obtain a product of a certain quality by controlling these factors depending on the purpose of use. These chemical components exert their respective effects, and by synergistic effects with each other, regulate the generation of plant hormones from the plant body, and at the same time, the growth promoting action by activating the enzyme in the plant and the specific action. Physiological activity mechanism of plants, such as growth control by selectively acting on enzymes, growth inhibition by enzyme inhibitors, enzyme inactivation prevention, growth promotion by removal of inhibitors or dissociation or substitution of inhibitors. Promote the improvement of.
【００２５】本発明において３槽式電解槽を使用して、
純水または電解質溶液を電解して活性酸素群を発生させ
ることが好ましい。カソード槽１、中間槽２、アノード
槽３からなる３槽式電解槽を用い、純水８を、中間槽２
にイオン交換樹脂４（DUPONT社製NAFION Superacid Cat
alysts Type NR50）を満たし、カーボン電極６、白金電
極７、イオオン交換膜５を用いて電解して、活性酸素群
を含む陰極水１２（酸性水、ｐＨ３．５）を得る場合の
電解条件の一例を表１に示し、３槽式電解槽の断面構造
の略図を図１に示す。In the present invention, a three-cell type electrolytic cell is used,
It is preferable to electrolyze pure water or an electrolyte solution to generate active oxygen groups. Using a three-cell type electrolytic cell consisting of a cathode cell 1, an intermediate cell 2 and an anode cell 3, pure water 8 is supplied to the intermediate cell 2
Ion-exchange resin 4 (NAFION Superacid Cat manufactured by DUPONT)
alysts Type NR50), and an example of electrolysis conditions for obtaining cathodic water 12 (acidic water, pH 3.5) containing active oxygen groups by electrolyzing using a carbon electrode 6, a platinum electrode 7, and an ion exchange membrane 5. Is shown in Table 1, and a schematic view of the cross-sectional structure of the three-cell type electrolytic cell is shown in FIG.
【００２６】[0026]
【表１】 [Table 1]
【００２７】[0027]
【実施例】以下本発明を実施例により、具体的に説明す
るが、本発明はこれら実施例によって限定されるもので
はない。 （実施例１）図１に示した３槽式電解槽を使用し、表１
の電解条件で純水８をカソード電解して陰極水１２（酸
性水、ｐＨ３．５）を得た。１はカソード槽、２は中間
槽、３はアノード槽、４はイオン交換槽、５はイオン交
換膜、６はカーボン電極（陰極）、７は白金電極（陽
極）、８は純水、１１は中性水、１０は陽極水（アルカ
リ水）を表す。次に、以下に示すようにして酵母抽出エ
キスを得た。前培養として、培養液［ Yeast Nitrogen
Base (Difco)＋１％(W/v) Glucose］５０mlに、供試菌
Saccharomyces cerevisiae 1FO-0234 1白金耳を接菌し
て、２７℃ overnight振とう培養した。この菌液を同上
の培養液９５０ｍｌに植菌し、同条件で本培養した。培
養後の菌液を遠心分離機にて２０００ｒｐｍ，５ｍｉｎ
遠沈した。上清除去後の沈殿物（菌体）に滅菌蒸留水を
加えよく懸濁し、同条件で遠沈した。この操作を３回繰
り返し沈殿物に培養液が残らないように洗浄した。湿菌
体（Wet weight :２０ｇ）を、５０％（ｖ／ｖ）エタノ
ール５０ｍｌに懸濁し、３０〜４０℃ overnight振とう
させながら自己融解させた。上記反応後の懸濁液は、
０．４５μｍのメンブランフィルターにてろ過した。ろ
液はエバポレーターにて６０℃減圧乾固し、その粉末 Y
east extract（成分組成を表２に示す）とした。表２中
の脂質の脂肪酸組成を分析（キャピラリーカラムを使用
したガスクロマトグラフ法）した結果、炭素数１４；０
が５．７％、炭素数１６；０が２７．１％、炭素数１
６；１（ｎ−７）が１５．５％、炭素数１８；０が１
２．７％、炭素数１８；１（ｎ−９）が２９．３％、炭
素数１８；２（ｎ−６）が９．７％であった。EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples. (Example 1) Using the 3-tank electrolytic cell shown in FIG.
Cathode electrolysis of pure water 8 was carried out under the electrolysis conditions of No. 1 to obtain cathode water 12 (acidic water, pH 3.5). 1 is a cathode tank, 2 is an intermediate tank, 3 is an anode tank, 4 is an ion exchange tank, 5 is an ion exchange membrane, 6 is a carbon electrode (cathode), 7 is a platinum electrode (anode), 8 is pure water, and 11 is Neutral water and 10 represent anode water (alkaline water). Next, a yeast extract was obtained as described below. As a pre-culture, culture medium [Yeast Nitrogen
Base (Difco) + 1% (W / v) Glucose] 50 ml, test bacteria
Saccharomyces cerevisiae 1FO-0234 1 platinum loop was inoculated and cultured at 27 ° C overnight with shaking. This bacterial solution was inoculated into 950 ml of the above-mentioned culture solution and main-cultured under the same conditions. Cultivated bacterial solution was centrifuged at 2000 rpm for 5 min
Spun off. Sterile distilled water was added to the precipitate (bacteria) after removal of the supernatant, and the suspension was well suspended and spun down under the same conditions. This operation was repeated 3 times, and the precipitate was washed so that the culture solution did not remain. Wet cells (Wet weight: 20 g) were suspended in 50 ml of 50% (v / v) ethanol and autolysed while shaking at 30-40 ° C overnight. The suspension after the above reaction is
It was filtered with a 0.45 μm membrane filter. The filtrate was dried at 60 ° C under reduced pressure using an evaporator and its powder Y
It was used as east extract (the composition of components is shown in Table 2). As a result of analyzing the fatty acid composition of the lipids in Table 2 (gas chromatography using a capillary column), the number of carbon atoms was 14; 0.
Is 5.7%, carbon number 16; 0 is 27.1%, carbon number 1
6; 1 (n-7) is 15.5%, carbon number 18; 0 is 1
2.7%, carbon number 18; 1 (n-9) was 29.3%, and carbon number 18; 2 (n-6) was 9.7%.
【００２８】[0028]
【表２】 [Table 2]
【００２９】前記の酸性水５００ｍｌに上記 Yeast ext
ractを５ｇ加え、超音波破砕機（島津製作所製 USP-30
0,300W/20KHz ）を使用して１５分間超音波を与え本発
明の植物生育調節剤ＡＲ１を作った。The above Yeast ext was added to 500 ml of the above acidic water.
Add 5g of ract, ultrasonic crusher (USP-30 manufactured by Shimadzu Corporation)
The plant growth regulator AR1 of the present invention was prepared by applying ultrasonic waves for 15 minutes at 0,300 W / 20 KHz).
【００３０】（実施例２）図２に示した３槽式電解槽を
使用し、アノード槽およびカソード槽に純水８、中間槽
２に８．０×１０-4％（ｗ／ｖ）ＮａＣ１水溶液９を通
水した。電圧１５Ｖ、流量：アノード槽１０００ｍｌ／
ｍｉｎ、中間槽５００ｍｌ／ｍｉｎ、カソード槽１００
０ｍｌ／ｍｉｎ、電極７は陰極、陽極いずれも白金を用
いた以外は表１の電解条件で電解して陽極に陽極水１０
（酸性水、ｐＨ２．７）（塩素イオン濃度２１０ｍｇ／
ｌ）を得た。１はカソード槽、２は中間槽、３はアノー
ド槽、４はイオン交換槽、５はイオン交換膜、７は白金
電極、８は純水、１１は中性水、１２は陰極水（アルカ
リ水）を表す。この酸性水１０００ｍｌに対し市販の植
物抽出エキス（熊笹抽出エキス）１０ｍｌを加え１５分
間攪拌する。この液を室温から加熱し、１００℃で約５
分間の熱処理を行って室温に戻し、０．４５μｍのメン
ブランフィルターにてろ過し、本発明の植物生育調節剤
ＡＲ２を作った。(Example 2) Using the three-cell type electrolytic cell shown in FIG. 2, pure water 8 was used for the anode and cathode cells, and 8.0 × 10 -4 % (w / v) NaC1 was used for the intermediate cell 2. Aqueous solution 9 was passed through. Voltage 15V, flow rate: Anode tank 1000ml /
min, intermediate tank 500 ml / min, cathode tank 100
0 ml / min. Electrode 7 was electrolyzed under the electrolysis conditions shown in Table 1 except that cathode and anode were both platinum.
(Acid water, pH 2.7) (Chloride ion concentration 210 mg /
l) was obtained. 1 is a cathode tank, 2 is an intermediate tank, 3 is an anode tank, 4 is an ion exchange tank, 5 is an ion exchange membrane, 7 is a platinum electrode, 8 is pure water, 11 is neutral water, 12 is cathode water (alkaline water). ) Represents. To 1000 ml of this acidic water, 10 ml of a commercially available plant extract (Kuma bamboo extract) was added and stirred for 15 minutes. This liquid is heated from room temperature to about 5 ° C at about 5 ° C.
After heat treatment for 1 minute, the mixture was returned to room temperature and filtered through a 0.45 μm membrane filter to prepare the plant growth regulator AR2 of the present invention.
【００３１】（実施例３）過酸化水素（Ｈ2 Ｏ2 ）濃度
１．０×１０-4％の水溶液５００ｍｌに、亜塩素酸ナト
リウム０．５ｍｇ、塩素酸カリウム０．３ｍｇ、実施例
１で用いた Yeastextract５ｇを溶解し、紫外線（２６
０ｎｍ）を４０分間照射し、本発明の植物生育調節剤Ａ
Ｒ３を作った。Example 3 In 500 ml of an aqueous solution of hydrogen peroxide (H 2 O 2 ) concentration of 1.0 × 10 −4 %, 0.5 mg of sodium chlorite and 0.3 mg of potassium chlorate were prepared. Dissolve 5 g of Yeastextract used, and use ultraviolet rays (26
0 nm) for 40 minutes to irradiate the plant growth regulator A of the present invention.
I made R3.
【００３２】（実施例４） （試験１）本発明の植物生育調節剤ＡＲ１〜３を用い
て、トマトの不定根伸長に及ぼす影響を調べた。 （材料及び試験方法）供試品種は、タキイ種苗（株）の
「強力米寿２号」を用い、１９９２年８月１７日に種子
を３日間催芽処理し東京農業大学熱帯園芸研究室のガラ
ス室で栽培した。８月２０日黒色ポリポット（直径９ｃ
ｍ、高さ８ｃｍ）に播種、２２日に出芽した。ポリポッ
トには、腐葉土と赤土深土を１：２の割合で混合し、石
灰１．６ｇを加えた。赤土深土は風乾状態にして、１ｃ
ｍ四方の篩を通して使用した。９月１０日に、約１０ｃ
ｍになった幼苗を地上１．５ｃｍの所で切取り、容量７
０ｍｌの試験管全体を黒ビニールで巻きアルミホイルで
覆った後、それぞれ水道水、本発明の植物生育調節剤Ａ
Ｒ１〜３の２５０倍希釈液、５００倍希釈液、計７区を
６０ｍｌずつ入れ、トマトの幼苗を切り口から約１．５
ｃｍの深さまで浸漬した。各区１０反復とし、２０℃、
２５００ｌｕｘの人工気象室内に静置して、７日後に不
定根の最大根長、根数を測定した。(Example 4) (Test 1) Using the plant growth regulators AR1 to 3 of the present invention, the effect on adventitious root elongation of tomato was examined. (Materials and test method) The test variety used was "Taikai Seed Co., Ltd.'s" Strong Yoneju No. 2 ", and the seeds were germinated for 3 days on August 17, 1992, and the glass room of the Tropical Horticulture Laboratory, Tokyo University of Agriculture was used. Cultivated in. August 20, black poly pot (diameter 9c
m, height 8 cm), and germinated on the 22nd. In the polypot, mulch and red soil were mixed at a ratio of 1: 2, and 1.6 g of lime was added. Red clay deep soil is air-dried, 1c
Used through a m-square sieve. September 10th, about 10c
Cut the seedlings that became m at a position 1.5 cm above the ground to a volume of 7
The entire 0 ml test tube was wrapped with black vinyl and covered with aluminum foil, then tap water and the plant growth regulator A of the present invention, respectively.
Put 60 ml of 250-fold diluted solution of R1-3, 500-fold diluted solution, total of 7 wards, and cut the tomato seedlings about 1.5 times from the cut end.
It was immersed to a depth of cm. Each section has 10 repetitions, 20 ℃,
After being left standing in a 2500 lux artificial weather room, the maximum root length and number of adventitious roots were measured 7 days later.
【００３３】ＡＲ１〜３を水で２５０倍および５００倍
に希釈した特殊活性水溶液を用いて栽培を行った場合、
トマトの不定根伸長に及ぼす影響を試験し、図３に根長
に及ぼす影響を棒グラフで、図５にＡＲ１〜３を水で２
５０倍に希釈して栽培を行った場合の不定根最根長に及
ぼす影響をグラフで、図６にＡＲ１〜３を水で５００倍
に希釈して栽培を行った場合の不定根最根長に及ぼす影
響をグラフで、図９に根数に及ぼす影響を棒グラフで、
図１０に根重に及ぼす影響を棒グラフで示す。各区１０
個体の平均値で表し、棒グラフで示した図中の縦棒は標
準偏差を、カッコ内の数字は対照区に対する％を示す。
対照区はＡＲ１〜３を使用しない場合である。When culturing is carried out using a special active aqueous solution obtained by diluting AR1 to AR3 with water 250 times and 500 times,
The effect on adventitious root elongation of tomato was tested, and the effect on root length is shown in a bar graph in FIG.
FIG. 6 is a graph showing the effect on the adventitious root maximum root length when 50-fold diluted and cultivated, and affects the adventitious root maximum root length when cultivated by diluting AR1-3 with water 500 times in FIG. The effect is shown in a graph, and the effect on the number of roots is shown in a bar graph in Fig. 9.
FIG. 10 shows a bar graph showing the influence on the root weight. Each ward 10
Represented by the average value of the individuals, the vertical bars in the figures shown as bar graphs represent the standard deviation, and the numbers in parentheses represent the% relative to the control group.
The control section is a case where AR1 to AR3 are not used.
【００３４】不定根長については、ＡＲ２５０倍区＞５
００倍区＞対照区の順に影響が大きく、根数、根重につ
いては、ＡＲ区の方が対照区より優れているが２５０倍
区、５００倍区にほとんど差はなかった。また、図７に
ＡＲ１〜３を水で２５０倍に希釈した特殊活性水溶液の
根重／全体重に及ぼす影響を、図８にＡＲ１〜３を水で
５００倍に希釈した特殊活性水溶液の根重／全体重に及
ぼす影響を示したが、ＡＲ２は地下部の、ＡＲ３は地上
部の成長が促進される事がわかった。Regarding indefinite root length, AR250 times> 5
The influence was large in the order of 00-fold> control, and the number of roots and root weight of AR were superior to those of the control, but there was almost no difference between 250-fold and 500-fold. Further, FIG. 7 shows the effect of the AR1-3 diluted 250 times with water on the root weight / total weight of the special active aqueous solution, and FIG. 8 shows the effect of the AR 1-3 diluted 500 times with the special active aqueous solution. Although the effect on the total weight was shown, it was found that AR2 promotes the growth of the underground part and AR3 promotes the growth of the above-ground part.
【００３５】一般に植物では、切断する事により切り口
付近で細胞***が始まることが知られている。上記の結
果から、切断するという刺激、ＡＲ１〜３特殊活性水溶
液中に含まれるラジカルによる刺激、発根を促進するフ
ェノール物質の作用とが、細胞***を誘導していると考
えられる。ＡＲ１〜３特殊活性水溶液を植物に与えるこ
とにより上記のような優れた結果が得られた理由は種々
考えられ、限定できないが、エチレン生成が促進され、
ＰＡＬ酵素（Phenylalanine Ammino Lylase ）が活性化
し、発根を促進させるフェノール物質が生成されること
によるものと考えられる。It is generally known that, in plants, cell division begins to occur near the cut edge when cut. From the above results, it is considered that the stimulation of cleavage, the stimulation by the radicals contained in the aqueous solutions of AR1 to 3 special activity, and the action of the phenolic substance that promotes rooting induce cell division. There are various possible reasons why the above-mentioned excellent results were obtained by feeding the plants with the AR 1-3 special active aqueous solution, but it is not limited, but ethylene production is promoted,
It is considered that this is because the PAL enzyme (Phenylalanine Ammino Lylase) is activated and a phenolic substance that promotes rooting is produced.
【００３６】（試験２）本発明の植物生育調節剤ＡＲ１
〜３の葉面散布がトマト幼苗のエチレン生成に及ぼす影
響について試験を行った。 （材料及び試験方法）供試品種および栽培方法は試験１
と同様で、１９９２年１０月１７日に種子を催芽処理
し、１０月２０日に播種、２３日に出芽し、１１月１７
日に処理を開始した。ＡＲ１〜３の５００倍希釈液をト
マト幼苗に葉面散布し、対照区には水道水を葉面散布し
てエチレンの生成量を毎日測定した。測定方法は、葉面
散布１時間後に、第３葉を切り取り、直径１ｃｍのコル
クボーラーで葉を打ち抜き、５０ｍｌの三角フラスコ内
に湿潤したろ紙を敷き、葉切片１０枚を入れた後、シリ
コ栓をし三角フラスコの全体を黒ビニールで巻いた。さ
らにアルミホイルで覆い、２５℃で６時間インキュベー
トし測定に供した。エチレン生成量については、ガスク
ロマトグラフ（島津製作所製ＧＣ−１４Ａ）を用い、カ
ラムはサンパックＡ、キャリヤガスはＮ2 （６ｋｇ／ｃ
ｍ2 ）、ディテクターはＦＩＤで１５０℃、インジェク
ターを１２０℃、カラムは８０℃で対照区、ＡＲ処理区
とも３反復にて測定を行い、平均値で表した。(Test 2) The plant growth regulator AR1 of the present invention
The effect of foliar application of ~ 3 on ethylene production in tomato seedlings was tested. (Material and test method) Test 1
Seeds were germinated on October 17, 1992, sown on October 20, sprouting on 23, November 17
Processing started on the day. A 500-fold diluted solution of AR1 to 3 was applied to the tomato seedlings on the leaves, and tap water was applied to the control areas to measure the amount of ethylene produced every day. One hour after the foliar application, the third leaf was cut off, the leaf was punched out with a cork borer with a diameter of 1 cm, a wet filter paper was laid in a 50 ml Erlenmeyer flask, and 10 leaf pieces were placed, followed by a silicon stopper. Then, the entire Erlenmeyer flask was wrapped with black vinyl. Further, it was covered with aluminum foil, incubated at 25 ° C. for 6 hours, and subjected to measurement. For the ethylene production amount, a gas chromatograph (GC-14A manufactured by Shimadzu Corporation) was used, the column was Sunpack A, and the carrier gas was N 2 (6 kg / c).
m 2 ), the detector was FID at 150 ° C., the injector was 120 ° C., and the column was at 80 ° C. The measurement was repeated 3 times for both the control group and the AR-treated group, and the average value was shown.
【００３７】ＡＲ１〜３の５００倍希釈液の葉面散布が
トマトのエチレン生成に及ぼす影響について図５に結果
を示した。ＡＲ１〜３の５００倍希釈液で処理すると７
時間後にエチレン生成量が増大し、２〜３日目にピーク
を迎えた。一般に、エチレンは植物の地上部の生育を抑
制して、根の生育を促進すること、またIignification
を促進し、クチクラ（cuticle ）蒸散を減少させて耐乾
性を高めたり、菌の侵入を防いで耐病性を高めたりする
ことが知られている。本試験２によりＡＲ１〜３の５０
０倍希釈液の葉面散布がトマトの健苗育成に利用できる
ことを示唆するものである。FIG. 5 shows the effect of foliar spraying of a 500-fold diluted solution of AR1-3 on ethylene production in tomato. 7 when treated with a 500-fold dilution of AR1-3
The amount of ethylene produced increased after the lapse of time, and reached a peak on the second to third days. In general, ethylene suppresses the growth of the aboveground parts of plants and promotes the growth of roots.
It is known to promote the above, reduce cuticle transpiration and increase drought resistance, and prevent invasion of bacteria to improve disease resistance. 50 of AR1 to 3 according to main test 2
This suggests that the foliar spray of the 0-fold diluted solution can be used for growing healthy tomato seedlings.
【００３８】試験２に準じて農家で行ったトマトの栽培
の結果、本発明の植物生育調節剤ＡＲを使用すると、ト
マトの節間伸長が抑制され、ずんぐり苗になり、発根、
殺菌力が増大することが観察されている。これらの結果
から、本発明の植物生育調節剤ＡＲ処理によりエチレン
生成が高まったためであると考えられる。このような本
発明の植物生育調節剤ＡＲの作用は農薬使用量の減少な
どに役立つものであり、また健苗育成に利用できること
を示唆するものである。As a result of cultivation of tomatoes carried out by a farmer in accordance with Test 2, when the plant growth regulator AR of the present invention was used, internodal elongation of tomatoes was suppressed to give rise to acorn seedlings, rooting,
Increased germicidal activity has been observed. From these results, it is considered that ethylene production was increased by the AR treatment of the plant growth regulator of the present invention. The action of the plant growth regulator AR of the present invention as described above is useful for reducing the amount of agricultural chemicals used, and suggests that it can be used for raising healthy seedlings.
【００３９】[0039]
【発明の効果】本発明は、植物生育調節剤、その製造方
法及びその使用方法に関するものであり、本発明の植物
生育促進剤をそのままであるいは水で１〜２０００倍に
希釈して植物に適用することにより、植物体からの植物
ホルモンであるエチレン、一般にフィトンチッド（フィ
トアレキシン）と呼ばれる揮発性物質の発生を調節する
と同時に、植物中の酵素を活性化することによる成長促
進作用、また特定の酵素に選択的に働く事による成長制
御作用、酵素阻害物質による成長抑制作用、酵素失活の
防止作用、阻害物質の除去ないし阻害物質の解離または
置換などによる成長促進作用などを調節して、植物の生
理活性機構の向上を促すことができる。純水、水道水な
どを用いて電解処理により活性酸素群を含む活性水を連
続的に作り、これと従来公知の各種の植物生理活性物質
を温和な条件下で反応させる簡単な方法により本発明の
植物生育調節剤を経済的に製造することができる。従来
農地で病害・害虫に対し様々な農薬などの薬剤が使用さ
れてきたが、本発明の植物生育調節剤を使用することに
より、それらの使用をなくするか、使用量を減少させる
ことができると共に、健苗育成にも利用できるので産業
上の利用価値が高い。INDUSTRIAL APPLICABILITY The present invention relates to a plant growth regulator, a method for producing the same, and a method for using the same. The plant growth promoter of the present invention is applied to plants as it is or after being diluted 1 to 2000 times with water. It regulates the generation of ethylene, which is a plant hormone from plants, and a volatile substance generally called phytoncide (phytoalexin), and at the same time, it promotes the growth-promoting action by activating enzymes in plants, and By controlling the growth control action by selectively acting on the enzyme, the growth inhibition action by the enzyme inhibitor, the enzyme inactivation prevention action, the growth promoting action by the removal of the inhibitor or the dissociation or substitution of the inhibitor, etc. It is possible to promote the improvement of the physiological activity mechanism of. The present invention is a simple method in which active water containing an active oxygen group is continuously produced by electrolytic treatment using pure water, tap water, etc., and this is reacted with various conventionally known plant physiologically active substances under mild conditions. The plant growth regulator can be produced economically. Conventionally, various agricultural chemicals such as pesticides have been used for diseases and pests in farmland, but by using the plant growth regulator of the present invention, it is possible to eliminate their use or reduce the amount used. At the same time, it can be used for raising healthy seedlings, so it has high industrial utility value.
【図１】 本発明で用いる３槽電解槽の断面を示す説明
図である。FIG. 1 is an explanatory view showing a cross section of a three-cell electrolytic cell used in the present invention.
【図２】 本発明で用いる他の３槽電解槽の断面を示す
説明図である。FIG. 2 is an explanatory view showing a cross section of another three-cell electrolytic cell used in the present invention.
【図３】 トマトの根長に及ぼす影響を示す棒グラフで
ある。FIG. 3 is a bar graph showing the influence on the root length of tomato.
【図４】 トマトのエチレン生成量に及ぼす影響を示す
グラフである。FIG. 4 is a graph showing the effect of tomato on ethylene production.
【図５】 トマトの不定根最根長に及ぼす影響を示すグ
ラフである。FIG. 5 is a graph showing the effect on the maximum root length of adventitious roots of tomato.
【図６】 トマトの不定根最根長に及ぼす影響を示すグ
ラフである。FIG. 6 is a graph showing the effect on the maximum root length of adventitious roots of tomato.
【図７】 トマトの（根重／全体重）に及ぼす影響を示
すグラフである。FIG. 7 is a graph showing the effect of tomato on (root weight / total weight).
【図８】 トマトの（根重／全体重）に及ぼす影響を示
すグラフである。FIG. 8 is a graph showing the effect of tomato on (root weight / total weight).
【図９】 トマトの根数に及ぼす影響を示す棒グラフで
ある。FIG. 9 is a bar graph showing the effect on the number of tomato roots.
【図１０】 トマトの根重に及ぼす影響を示す棒グラフ
である。FIG. 10 is a bar graph showing the influence on the root weight of tomato.
１ カソード槽 ２ 中間槽 ３ アノード槽 ４ イオン交換樹脂 ５ イオン交換膜 ６ カーボン電極 ７ 白金電極 ８ 純水 ９ 食塩水 １０ 陽極水 １１ 中性水 １２ 陰極水 1 Cathode tank 2 Intermediate tank 3 Anode tank 4 Ion exchange resin 5 Ion exchange membrane 6 Carbon electrode 7 Platinum electrode 8 Pure water 9 Saline solution 10 Anode water 11 Neutral water 12 Cathode water
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ａ０１Ｎ 61:00） (Ａ０１Ｎ 59/00 63:02） (Ａ０１Ｎ 59/00 65:00） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification code Office reference number FI technical display location A01N 61:00) (A01N 59/00 63:02) (A01N 59/00 65:00)
Claims (6)
よびその重合物、不飽和脂肪酸を含む微生物抽出液また
は植物抽出液、有機質肥料、有害物質を除いた活性汚
泥、ビタミン、ユビキノンからなる群から選択される少
なくとも一種と、 3Ｏ2 の一電子還元種であるスーパー
オキシド（Ｏ2 -）、励起状態の酸素分子である励起三重
項酸素（ 3Ｏ2 *）、一重項酸素（ 1Ｏ2 ）、ヒドロキシ
ラジカル（・ＯＨ）、オゾン（Ｏ3 ）、金属オキソ種を
含む金属−酸素錯体、過酸化水素（Ｈ2 Ｏ2 ）、次亜塩
素酸、亜塩素酸、塩素酸、過塩素酸、ペルオキシラジカ
ル（ＬＯＯ・）、アルコキシラジカル（ＬＯ・）、ヒド
ロペルオキシド（ＬＯＯＨ）から選択される少なくとも
一種の活性酸素群との反応で生成した、過酸化物を含む
植物生育調節剤。1. An unsaturated fatty acid and its ester compound, and its polymer, a microorganism extract or plant extract containing unsaturated fatty acid, organic fertilizer, activated sludge excluding harmful substances, vitamins and ubiquinone. is the at least one and, superoxide an electron reduced species of 3 O 2 (O 2 -) , oxygen molecules in the excited state triplet oxygen (3 O 2 *), singlet oxygen (1 O 2) , Hydroxy radical (.OH), ozone (O 3 ), metal-oxygen complex containing metal oxo species, hydrogen peroxide (H 2 O 2 ), hypochlorous acid, chlorous acid, chloric acid, perchloric acid, A plant growth regulator containing a peroxide produced by a reaction with at least one active oxygen group selected from a peroxy radical (LOO.), An alkoxy radical (LO.) And a hydroperoxide (LOOH).
理、化学的処理（薬剤添加）からなる群から選ばれる少
なくとも一種の方法で純水または電解質溶液を処理して
請求項１記載の活性酸素群を発生させ、不飽和脂肪酸と
そのエステル化合物、およびその重合物、不飽和脂肪酸
を含む微生物抽出液または植物抽出液、有機質肥料、有
害物質を除いた活性汚泥、ビタミン、ユビキノンからな
る群から選択される少なくとも一種と反応させることを
特徴とする植物生育調節剤の製造方法。2. The active oxygen according to claim 1, wherein pure water or an electrolyte solution is treated by at least one method selected from the group consisting of electrolytic treatment, ultrasonic treatment, ultraviolet irradiation treatment, and chemical treatment (chemical addition). Group is selected from the group consisting of unsaturated fatty acids and their ester compounds, and their polymers, microbial or plant extracts containing unsaturated fatty acids, organic fertilizers, activated sludge excluding harmful substances, vitamins, and ubiquinone. A method for producing a plant growth regulator, comprising reacting with at least one of the above.
解質溶液を電解することにより活性酸素群を発生させる
ことを特徴とする請求項２記載の植物生育調節剤の製造
方法。3. The method for producing a plant growth regulator according to claim 2, wherein the active oxygen group is generated by electrolyzing pure water or an electrolytic solution using a three-cell type electrolytic cell.
項２あるいは請求項３記載の植物生育調節剤の製造方
法。4. The method for producing a plant growth regulator according to claim 2, further comprising heat treatment.
とを特徴とする請求項２ないし請求項４記載の植物生育
調製剤の製造方法。5. The method for producing a plant growth preparation according to claim 2, wherein the pH is further adjusted to pH 5 to 7.
２０００倍に水で希釈して植物に適用することを特徴と
する植物生育調節剤の使用方法。6. The plant growth regulator according to claim 1, wherein
A method of using a plant growth regulator, which comprises diluting 2000 times with water and applying to a plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12220093A JP3639875B2 (en) | 1993-04-27 | 1993-04-27 | Plant growth regulator treated with active oxygen group, its production method and its use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12220093A JP3639875B2 (en) | 1993-04-27 | 1993-04-27 | Plant growth regulator treated with active oxygen group, its production method and its use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06305921A true JPH06305921A (en) | 1994-11-01 |
| JP3639875B2 JP3639875B2 (en) | 2005-04-20 |
Family
ID=14830041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12220093A Expired - Lifetime JP3639875B2 (en) | 1993-04-27 | 1993-04-27 | Plant growth regulator treated with active oxygen group, its production method and its use |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3639875B2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997041732A1 (en) * | 1996-05-07 | 1997-11-13 | Hiroshi Kawai | Effervescent preparation for plants |
| WO1998051155A1 (en) * | 1997-05-09 | 1998-11-19 | Hiroshi Kawai | Method of preventing plant diseases and composition therefor |
| WO2002049969A3 (en) * | 2000-12-21 | 2003-01-30 | Fraunhofer Ges Forschung | Method for accelerating biocatalytic and/or hormonal processes and use of the same |
| WO2008146873A1 (en) | 2007-05-30 | 2008-12-04 | Nisshin Pharma Inc. | Plant growth promoter |
| CN105399501A (en) * | 2015-11-24 | 2016-03-16 | 陕西省微生物研究所 | Trunk-injection medicament for controlling branch and trunk diseases of fruit trees, and preparation method for trunk-injection medicament |
| CN109688821A (en) * | 2016-09-08 | 2019-04-26 | 揖斐电株式会社 | The manufacturing method of plant activator and plant activator |
-
1993
- 1993-04-27 JP JP12220093A patent/JP3639875B2/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997041732A1 (en) * | 1996-05-07 | 1997-11-13 | Hiroshi Kawai | Effervescent preparation for plants |
| WO1998051155A1 (en) * | 1997-05-09 | 1998-11-19 | Hiroshi Kawai | Method of preventing plant diseases and composition therefor |
| WO2002049969A3 (en) * | 2000-12-21 | 2003-01-30 | Fraunhofer Ges Forschung | Method for accelerating biocatalytic and/or hormonal processes and use of the same |
| WO2008146873A1 (en) | 2007-05-30 | 2008-12-04 | Nisshin Pharma Inc. | Plant growth promoter |
| CN105399501A (en) * | 2015-11-24 | 2016-03-16 | 陕西省微生物研究所 | Trunk-injection medicament for controlling branch and trunk diseases of fruit trees, and preparation method for trunk-injection medicament |
| CN109688821A (en) * | 2016-09-08 | 2019-04-26 | 揖斐电株式会社 | The manufacturing method of plant activator and plant activator |
| EP3510868A4 (en) * | 2016-09-08 | 2020-04-08 | Ibiden Co., Ltd. | Plant activator and process for producing plant activator |
| US11382281B2 (en) | 2016-09-08 | 2022-07-12 | Ibiden Co., Ltd. | Plant activator and a method of manufacturing the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3639875B2 (en) | 2005-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3458948B2 (en) | Composition for spraying plant foliage and method of using the same | |
| KR101352628B1 (en) | Mineral nutrition solution and method for cultivating functional plant using thereof | |
| CN102106229B (en) | Method for producing original ecological organic vegetables | |
| WO2023025115A1 (en) | Application of 2-amino-3-methylhexanoic acid in promoting plant growth and increasing yield | |
| CN109699675A (en) | A method of Ozone Water being made with water and replaces pesticide and is replaced with Pipe spraying manually spraying | |
| JP4200455B2 (en) | Electrolyzed reduced water generation device for crop cultivation, foliar spray water, soil irrigation water or soil irrigation water, and crop cultivation method | |
| WO2023155834A1 (en) | Use of 2-amino-3-hydroxy-3-methylbutyric acid in promoting plant growth | |
| CN101020766A (en) | Degradable mulching product and its production process | |
| EP1623623A1 (en) | Antibacterial antiviral composition | |
| CN114794113A (en) | Use of 2-amino-3-phenylbutyric acid or derivatives thereof as plant growth regulators | |
| KR102332750B1 (en) | Functional Crops Cultivation Method Using Nano Organic Germanium And Nano Organic Selenium | |
| JP3639875B2 (en) | Plant growth regulator treated with active oxygen group, its production method and its use | |
| JP2017038562A (en) | Symbiosis promoting method and symbiosis promoter | |
| CN101933452A (en) | Method for sterilizing soil matrix used for nursery stock | |
| KR20010068188A (en) | Method for growing contamination free and functional bean sprouts | |
| JPH0645523B2 (en) | Growth promotion supplementary water for plant cultivation of agricultural products | |
| JP7327922B2 (en) | Agent for preventing or improving soil damage | |
| JP2022083395A (en) | Plant disease control agent and plant disease control method | |
| JP5223132B2 (en) | Plant pathogen infection inhibitor and method for suppressing pathogen infection | |
| JP6546685B1 (en) | Method of producing soil disinfectant | |
| JP2002335764A (en) | Cultivation method where electrolytic water is alternately sprinkled | |
| JPH09175919A (en) | Prevention of pyricularia oryzae and composition therefor | |
| JPS6360904A (en) | Growth promoting adjuvant water for cultivating plant such as field crop or the like | |
| EP3005872A1 (en) | Product for regulating plant health, method for obtaining said product and use thereof | |
| JPH1175549A (en) | Composition for improving food nutrient ingredient of plant and improvement in food nutrient ingredient of plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20040601 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20040802 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20041221 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20041224 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090128 Year of fee payment: 4 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100128 Year of fee payment: 5 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110128 Year of fee payment: 6 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110128 Year of fee payment: 6 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120128 Year of fee payment: 7 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120128 Year of fee payment: 7 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130128 Year of fee payment: 8 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140128 Year of fee payment: 9 |
|
| EXPY | Cancellation because of completion of term |