JPH07238488A - Production of compound material of fiber and bacterial cellulose and composite material produced by the process - Google Patents
Production of compound material of fiber and bacterial cellulose and composite material produced by the processInfo
- Publication number
- JPH07238488A JPH07238488A JP2672894A JP2672894A JPH07238488A JP H07238488 A JPH07238488 A JP H07238488A JP 2672894 A JP2672894 A JP 2672894A JP 2672894 A JP2672894 A JP 2672894A JP H07238488 A JPH07238488 A JP H07238488A
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- cellulose
- paper
- genus
- fiber
- pomace
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、繊維−バクテリアセル
ロース複合体及びその製造方法に係わり、特に、該複合
体をパルプ原料とした非木材紙を提供する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fiber-bacterial cellulose composite and a method for producing the same, and more particularly to a non-wood paper using the composite as a pulp raw material.
【０００２】[0002]
【従来の技術】森林資源は伐採量の拡大、酸性雨及び環
境汚染などの要因から減少の一途をたどっている。伐採
された森林資源、即ち木材の消費量の約１／６はパルプ
工業（製紙業）によるものであり、環境及び資源保護の
目的から再生紙や非木材紙（木材以外の植物繊維から得
たセルロースをパルプ原料とする紙）の製造技術の確立
及び進歩が求められている。2. Description of the Related Art Forest resources are declining due to factors such as an increase in the amount of logging, acid rain and environmental pollution. About one-sixth of the forest resources that have been cut down, that is, the amount of wood consumed, comes from the pulp industry (paper making industry), and recycled paper and non-wood paper (obtained from plant fibers other than wood) for the purpose of environmental and resource protection There is a demand for the establishment and advancement of a production technology of paper using cellulose as a pulp raw material.
【０００３】非木材紙としては、農業廃棄物（例えばサ
トウキビ、タケ、アサ、ムギ、イネ、及びトウモロコシ
などの農作物において、実の収穫後に留置される藁状繊
維等）を利用しパルプ原料を得る方法が公知であり、中
でも、バガス紙については現在あらゆるグレードの紙
（例えば、グラシン紙、ダンボール中芯、ライナー、白
板紙、袋、包装紙、筆記用紙、印刷用紙、トイレット、
ティシュ、タオル及び塗工用原紙等）が実用化されてい
る。[0003] As the non-wood paper, pulp waste is obtained by using agricultural waste (for example, straw-shaped fibers left after fruit harvest in agricultural products such as sugar cane, bamboo, hemp, wheat, rice, and corn). Methods are known, among them, for bagasse paper, currently all grades of paper (e.g. glassine paper, cardboard core, liner, white paperboard, bag, wrapping paper, writing paper, printing paper, toilet,
Tissues, towels, base paper for coating, etc.) have been put to practical use.
【０００４】[0004]
【発明が解決しようとする課題】バガスとは一般に、甘
蔗茎（サトウキビ）から砂糖原料を抽出した後の搾りカ
スの総称であり、東南アジア地方では古くから重要なパ
ルプ資源である。[Problems to be Solved by the Invention] Generally, bagasse is a general term for squeezed residue after extracting a sugar raw material from sugar cane, and has been an important pulp resource in Southeast Asia since ancient times.
【０００５】しかしながら、バガス紙は木材由来の紙に
くらべて脆弱であるという欠点を有しているため、現在
実用化されているバガス紙にあっても、通常３０−７０
％程度の木材パルプが混合されているか又は補強材など
が利用されている。However, since bagasse paper has a drawback that it is more fragile than wood-derived paper, even bagasse paper currently in practical use is usually 30-70.
% Wood pulp is mixed or a reinforcing material is used.
【０００６】また、バガスには通常約２０％の糖分が含
有されているため、これらの洗浄・抽出廃液中のＢＯＤ
（生物学的酸素要求量）及びＣＯＤ（化学的酸素要求
量）は膨大なものとなり、廃液処理工程に大きな負荷を
与える。Further, since bagasse usually contains about 20% of sugar, BOD in the cleaning / extracting waste liquid of these is
(Biological oxygen demand) and COD (chemical oxygen demand) become enormous, which gives a large load to the waste liquid treatment process.
【０００７】従って、農業廃棄物を利用した非木材紙
（バガス紙）にあっては、より一層の改良が望まれてい
る。Therefore, further improvement is desired for non-wood paper (bagasse paper) using agricultural waste.
【０００８】[0008]
【課題を解決するための手段】本発明者らは、バガスが
含有する炭素源及び窒素源に着目し、セルロース生産性
微生物を接種し、培養することで該微生物にバクテリア
セルロースを産生させることができ、且つバガス中の粗
荒な繊維を架橋する様にバクテリアセルロースの微細な
繊維が形成され、バガスの繊維状態を改善でき製紙後の
強度が増強されることを見い出し、本発明を完成させ
た。[Means for Solving the Problems] The present inventors focused on the carbon source and nitrogen source contained in bagasse, and inoculated and cultivated a cellulose-producing microorganism to cause the microorganism to produce bacterial cellulose. It was found that fine fibers of bacterial cellulose can be formed so as to cross-link coarse fibers in bagasse, the fiber state of bagasse can be improved, and the strength after papermaking can be enhanced, and the present invention has been completed. .
【０００９】即ち本発明は、繊維とセルロース生産性微
生物が資化可能な炭素源及び／又は窒素源とを含有す
る、植物由来のセルロース主体組成物中にセルロース生
産性微生物を接種し、培養することを特徴とする繊維−
バクテリアセルロース複合体の製造方法である。That is, according to the present invention, a plant-derived cellulose-based composition containing a fiber and a carbon source and / or a nitrogen source that can be assimilated by a cellulose-producing microorganism is inoculated with the cellulose-producing microorganism and cultured. Fiber characterized by
It is a method for producing a bacterial cellulose complex.
【００１０】本発明で使用される植物由来のセルロース
主体組成物は、繊維並びに炭素源及び／又は窒素源を含
有するものである。The plant-derived cellulose-based composition used in the present invention contains a fiber and a carbon source and / or a nitrogen source.
【００１１】ここでいう繊維とは、実質的に、セルロー
ス及びセルロースを主鎖としたヘテロ多糖を含むもの並
びにβ−１，３、β−１，２等のグルカンを含むもので
あり、ヘテロ多糖の場合のセルロース以外の構成成分
は、マンノース、フルクトース、ガラクトース、キシロ
ース、アラビノース、ラムノース、グルクロン酸等の六
炭糖五炭糖及び有機酸等である。The term "fiber" as used herein means substantially a substance containing cellulose and a heteropolysaccharide having cellulose as a main chain and a substance containing glucans such as β-1,3, β-1,2, and the like. In the case of, the constituent components other than cellulose are hexoses such as mannose, fructose, galactose, xylose, arabinose, rhamnose and glucuronic acid, and organic acids.
【００１２】尚、これらの多糖は単一物質である場合も
あるし、２種以上の多糖が水素結合等により混在しても
良い。Incidentally, these polysaccharides may be a single substance, or two or more kinds of polysaccharides may be mixed by hydrogen bonding or the like.
【００１３】これら繊維並びに炭素源及び／又は窒素源
を含有する、植物由来のセルロース主体組成物の具体的
な例としては、サトウキビ搾りかす（バガス）、小麦ふ
すま、大豆搾りかす、オカラ、籾がら、茶がら、麦芽搾
りかす、コーヒー豆搾りかす、ビート（砂糖大根）搾り
かす、果実搾りかす、果実皮及びセルラーゼ処理で一部
糖化したオガクズ、古紙等が挙げられる。Specific examples of a plant-derived cellulose-based composition containing these fibers and a carbon source and / or a nitrogen source include sugar cane pomace (bagasse), wheat bran, soybean pomace, okara, and chaff. , Tea powder, malt pomace, coffee bean pomace, beet (sugar radish) pomace, fruit pomace, fruit peel and sawdust partially saccharified by cellulase treatment, waste paper and the like.
【００１４】尚、セルロース主体組成物を常法によりパ
ルプ化した後、酢酸菌等を接種して培養することも可能
である。It is also possible to pulp the cellulose-based composition by a conventional method and then inoculate it with acetic acid bacteria or the like and culture it.
【００１５】セルロース主体組成物中の炭素源及び窒素
源の濃度は様々な値を取り得るので、セルロース生産性
微生物がセルロースを生産するのに十分なレベルに満た
ない場合は、当業者によって適宜炭素源、窒素源及び各
種栄養素が添加されよう。Since the concentrations of the carbon source and the nitrogen source in the cellulose-based composition can take various values, if the cellulosic microorganism does not reach a sufficient level to produce cellulose, those skilled in the art can appropriately select carbon. Sources, nitrogen sources and various nutrients will be added.
【００１６】炭素源としてはシュクロース、グルコー
ス、グルコン酸、フルクトース、マンニトール、ソルビ
トール、ガラクトース、マルトース、エリスリット、カ
ドニット、グリセリン、エテレングリコール、プロピレ
ングリコール、エタノール、プロパノール、ブタノー
ル、酢酸、酪酸及び吉草酸等が挙げられる。Examples of carbon sources include sucrose, glucose, gluconic acid, fructose, mannitol, sorbitol, galactose, maltose, erythritol, cadnit, glycerin, ethene glycol, propylene glycol, ethanol, propanol, butanol, acetic acid, butyric acid, and citrate. Herb acid and the like can be mentioned.
【００１７】窒素源としては硫酸アンモニウム、塩化ア
ンモニウム、リン酸アンモニウム等のアンモニウム塩、
硝酸塩、尿素及びペプトン等の有機又は無機の窒素化合
物が挙げられる。As the nitrogen source, ammonium salts such as ammonium sulfate, ammonium chloride and ammonium phosphate,
Organic or inorganic nitrogen compounds such as nitrates, urea and peptone are mentioned.
【００１８】これら組成物には、天然の炭素源及び／又
は窒素源が含有されているが、培養に際して更に天然及
び／又は人工のものを添加することが可能である。These compositions contain a natural carbon source and / or nitrogen source, but it is possible to add a natural and / or artificial one during the culture.
【００１９】更に、添加する栄養素として、アミノ酸、
ビタミン、脂肪酸、核酸、更にこれらのものを含有する
ペプトン、カザミノ酸、酵母エキス及び豆濃等を添加す
ることも可能であり、この他に２，７，９−トリカルボ
キシ−１Ｈピロロ［２，３−Ｓ］−キノリン−４，５−
ジオン、ピロロキノリンキノン（ＰＱＱ）、イノシトー
ル及び／又はフィチン酸もセルロース生成促進因子とし
て添加するとバクテリアセルロースの生産性向上に効果
がある。Further, as nutrients to be added, amino acids,
It is also possible to add vitamins, fatty acids, nucleic acids, and further peptone, casamino acid, yeast extract, tofu containing these, etc., in addition to these, 2,7,9-tricarboxy-1H pyrrolo [2, 3-S] -quinoline-4,5-
Addition of dione, pyrroloquinoline quinone (PQQ), inositol and / or phytic acid as a cellulose production promoting factor is effective in improving bacterial cellulose productivity.
【００２０】本発明で使用される『セルロース生産性微
生物』とは、糖類を代謝しセルロース性物質を産生する
能力を有する微生物であり、この微生物によって生産さ
れるセルロース性物質を『バクテリアセルロース』とい
う。The "cellulose-producing microorganism" used in the present invention is a microorganism having the ability to metabolize sugars to produce a cellulosic substance, and the cellulosic substance produced by this microorganism is called "bacterial cellulose". .
【００２１】より具体的には、Ａｃｅｔｏｂａｃｔｅｒ
属、Ａｇｒｏｂａｃｔｅｒｉｕｍ属、Ｒｈｉｚｏｂｉｕ
ｍ属、Ｓａｒｃｉｎａ属、Ｐｓｅｕｄｏｍｏｎａｓ属、
Ａｃｈｒｏｍｏｂａｃｔｅｒ属、Ａｌｃａｌｉｇｅｎｅ
ｓ属、Ａｅｒｏｂａｃｔｅｒ属、Ａｚｏｔｏｂａｃｔｅ
ｒ属、Ｚｏｏｇｌｅａ属に属する細菌及び藻類等が挙げ
られる。More specifically, Acetobacter
Genus, Agrobacterium, Rhizobiu
m genus, Sarcina genus, Pseudomonas genus,
Genus Achromobacter, Alcaligene
s genus, Aerobacterium genus, Azotobacte
Examples thereof include bacteria belonging to the genus r and Zooglea, and algae.
【００２２】これらの中でもＡｃｅｔｏｂａｃｔｅｒ属
に属する酢酸菌（例えば、ＢＰＲ２００１株、ＡＴＣＣ
２３７６８株、ＡＴＣＣ２３７６９株、ＡＴＣＣ１４８
５１株、ＡＴＣＣ１０２４５株、ＡＴＣＣ１１１４２株
及びＡＴＣＣ１０８２１株）を用いることが好ましく、
より好ましくはＢＰＲ２００１株である。Among these, acetic acid bacteria belonging to the genus Acetobacter (eg, BPR2001 strain, ATCC
23768 strain, ATCC23769 strain, ATCC148
51 strains, ATCC10245 strain, ATCC11142 strain and ATCC10821 strain),
More preferably, it is BPR2001 strain.
【００２３】ここでいうＢＰＲ２００１株の微生物は、
平成５年２月２４日付で通産省工業技術院生命工学工業
技術研究所に寄託されている（ＦＥＲＭ Ｐ−１３４６
６）。更に、該微生物は平成６年２月７日付でブダペス
ト条約に基づく寄託へ移管されている（ＦＥＲＭ ＢＰ
−４５４５）。The microorganism of the BPR2001 strain referred to here is
Deposited at the Institute of Biotechnology, Institute of Industrial Science and Technology, Ministry of International Trade and Industry on February 24, 1993 (FERM P-1346.
6). Furthermore, the microorganism was transferred to a deposit under the Budapest Treaty on February 7, 1994 (FERM BP
-4545).
【００２４】これらセルロース生産性微生物を植物由来
のセルロース主体組成物に接種し、培養する際には、雰
囲気中の酸素濃度、ｐＨ及び温度等の培養条件を適宜、
設定する必要がある。When these cellulose-producing microorganisms are inoculated into a plant-derived cellulose-based composition and cultured, the culture conditions such as oxygen concentration in the atmosphere, pH and temperature are appropriately changed.
Must be set.
【００２５】培養中のｐＨとしては、３−７の範囲内が
使用可能であり、好ましくは４−６、より好ましくは５
付近のｐＨである。The pH during the culture can be within the range of 3-7, preferably 4-6, and more preferably 5.
The pH is in the vicinity.
【００２６】ｐＨを調整する際には、あらゆるアルカリ
及び酸を用いることが可能である。例えば、水酸化ナト
リウム、水酸化カリウム、及び炭酸ナトリウム等のアル
カリ、並びに酢酸、クエン酸、塩酸、硫酸及び硝酸等の
酸が使用可能である。Any alkali and acid can be used in adjusting the pH. For example, alkalis such as sodium hydroxide, potassium hydroxide, and sodium carbonate, and acids such as acetic acid, citric acid, hydrochloric acid, sulfuric acid, and nitric acid can be used.
【００２７】培養中の温度としては、１０−５０℃の範
囲内で行うことが可能であり、好ましくは１０−４０
℃、より好ましくは２０−４０℃、さらに好ましくは２
５−３５℃で行うことが可能である。The temperature during the culture can be carried out within the range of 10-50 ° C, preferably 10-40.
℃, more preferably 20-40 ℃, more preferably 2
It can be performed at 5-35 ° C.
【００２８】また、培養方法としては、通気攪拌、振
盪、静置培養のいずれでも良く、培養槽に供給する酸素
濃度は１−１００％、好ましくは２０−８０％、より好
ましくは２５−７５％であれば良い。The culture method may be any of aeration stirring, shaking, and static culture, and the oxygen concentration supplied to the culture tank is 1-100%, preferably 20-80%, more preferably 25-75%. If it is good.
【００２９】このような培養方法については、特開昭６
２−２６５９９０号公報及び特開昭６１−２２１２０１
号公報等の他に、本出願人名義の特願平５−４６８４４
号明細書及び特願平５−３３１４９１号明細書に具体例
が記載されている。Regarding such a culture method, Japanese Patent Laid-Open No.
2-265990 and Japanese Patent Laid-Open No. 61-221201.
Japanese Patent Application No. 5-46844 in the name of the present applicant
Specific examples are described in Japanese Patent Application No. 5-331491.
【００３０】また本発明は、前記製造方法によって得ら
れ得る繊維−バクテリアセルロース複合体にも係わる。The present invention also relates to a fiber-bacterial cellulose composite obtainable by the above production method.
【００３１】前記製造方法より得た複合体を、その培養
液中から精製するには、例えば、水洗、加圧脱水、希酸
洗浄、アルカリ洗浄、有機溶媒による処理、次亜塩素酸
ソーダー及び過酸化水素等の漂白剤による処理、リゾチ
ーム等の菌体溶解酵素による処理、ラウリル硫酸ソーダ
ー、デオキシコール酸等の界面活性剤による処理、並び
に常温から２００℃の範囲の加熱洗浄等の単独及び併用
して施すことにより行える。To purify the complex obtained by the above-mentioned production method from the culture solution thereof, for example, washing with water, pressure dehydration, washing with dilute acid, washing with an alkali, treatment with an organic solvent, sodium hypochlorite and peroxide are carried out. Treatment with a bleaching agent such as hydrogen oxide, treatment with a lytic enzyme such as lysozyme, treatment with a surfactant such as sodium lauryl sulfate and deoxycholic acid, and heat washing in the range of room temperature to 200 ° C, alone or in combination It can be done by applying.
【００３２】更に本発明は、繊維−バクテリアセルロー
ス複合体の成形物、例えば紙、建材、セロハン、アスフ
ァルト及び壁材なども提供する。特に、該複合体による
紙は本発明の好ましい実施態様の一つである。The present invention also provides molded articles of fiber-bacterial cellulose composites such as paper, building materials, cellophane, asphalt and wall materials. In particular, the composite paper is one of the preferred embodiments of the present invention.
【００３３】該複合体を成形し、紙とする際には、通常
の製紙方法で行うことができる。パルプ化工程ではメカ
ニカルパルプ化法、サーモメカニカルパルプ化法、化学
メカニカルパルプ化法、半化学パルプ化法、化学パルプ
化法及び可溶化パルプ化法等を採る事ができるが、バク
テリアセルロースの変性・変化を抑制するためにメカニ
カルパルプ又はサーモメカニカルパルプ化法によること
が好ましい。When the composite is formed into a paper, it can be carried out by an ordinary papermaking method. In the pulping process, mechanical pulping method, thermo-mechanical pulping method, chemical mechanical pulping method, semi-chemical pulping method, chemical pulping method, solubilizing pulping method, etc. can be adopted, but modification of bacterial cellulose In order to suppress the change, it is preferable to use a mechanical pulp or thermomechanical pulping method.
【００３４】以下、実施例によって本発明を詳細に説明
するが、本発明はこの範囲に限定されるものではない。The present invention is described in detail below with reference to examples, but the present invention is not limited to this range.
【００３５】[0035]
（実施例１）植付１年後のサトウキビを、砂糖の生産で
通常用いられている方法に従って圧搾して糖液を搾り出
し、サトウキビ搾りカス（バガス）を得た。(Example 1) Sugarcane one year after planting was squeezed according to a method usually used in sugar production to squeeze out a sugar solution to obtain sugarcane squeezed residue (bagasse).
【００３６】このようにして得たバガスをハサミで約５
ｍｍ角に切削し、その１００部に水２５部を加えて混合
し、酢酸を用いてｐＨ＝５．５に調整した後、酢酸菌Ａ
ｃｅｔｏｂａｃｔｅｒ ｓｐ． ＢＰＲ２００１を１０
4 ｃｅｌｌ／ｇとなるように接種した。The bagasse obtained in this manner was scooped for about 5 times.
After cutting into a square mm, 25 parts of water was added to 100 parts of the mixture and mixed, and the pH was adjusted to 5.5 using acetic acid.
cetobacter sp. BPR2001 10
The cells were inoculated at 4 cells / g.
【００３７】これを２８℃に保温しながら時々全体をか
き混ぜ、あるいはそのまま静置し、培養した。１週間
後、バガスの繊維部分を光学顕微鏡で観察すると、酢酸
菌が繁殖していた。さらにこれらを走査型電子顕微鏡で
観察するとバクテリアセルロースの繊維が、バガスの表
面にその繊維を架橋するように付着していた（バガス繊
維−バクテリアセルロース複合体が形成された）。While keeping the mixture at 28 ° C., the whole was occasionally stirred or left as it was for culturing. One week later, when the fiber portion of bagasse was observed with an optical microscope, acetic acid bacteria were growing. Further, when these were observed with a scanning electron microscope, the fibers of bacterial cellulose adhered to the surface of bagasse so as to crosslink the fibers (a bagasse fiber-bacterial cellulose complex was formed).
【００３８】（実施例２）実施例１で調製したバガス繊
維−バクテリアセルロース複合体を製紙用ダブルディス
クリファイナーでパルプ化し、常法に従って洗浄・漂白
した後、紙を作成した。この紙をＡ紙とした。(Example 2) The bagasse fiber-bacterial cellulose composite prepared in Example 1 was pulped with a double disk refiner for papermaking, washed and bleached by a conventional method, and then paper was prepared. This paper was designated as A paper.
【００３９】（実施例３）実施例１で５ｍｍ角に切削し
たバガスを、そのまま実施例２と同様の手順でパルプ化
した。これに酢酸菌ＢＰＲ２００１株を接種して実施例
１と同様に培養した。１週間後、バガスの繊維部分を走
査型電子顕微鏡で観察すると、実施例１と同じようにバ
ガス繊維−バクテリアセルロース複合体が形成されてい
た。Example 3 The bagasse cut into 5 mm square in Example 1 was pulped as it was in the same procedure as in Example 2. The acetic acid bacterium BPR2001 strain was inoculated into this and cultured in the same manner as in Example 1. After one week, when the fiber portion of bagasse was observed with a scanning electron microscope, a bagasse fiber-bacterial cellulose complex was formed as in Example 1.
【００４０】このようにして得たバガス繊維−バクテリ
アセルロース複合体を、実施例２と同様の手順で製紙し
た。この紙をＢ紙とした。The bagasse fiber-bacterial cellulose composite thus obtained was paper-made by the same procedure as in Example 2. This paper is referred to as paper B.
【００４１】（実施例４）実施例１で５ｍｍ角に切削し
たバガスを、そのまま実施例２と同様の手順でパルプ化
し、バガス繊維のみからなる紙を作成した。これをバガ
ス紙とした。このバガス紙は脆弱であり、引張りにより
容易に切断することができた。Example 4 The bagasse cut into 5 mm square in Example 1 was pulped as it was in the same procedure as in Example 2 to prepare a paper consisting of bagasse fiber only. This was used as bagasse paper. This bagasse paper was fragile and could be easily cut by pulling.
【００４２】次に、Ａ紙、Ｂ紙及びバガス紙の引張強度
をＪＩＳ Ｐ８１１３（１９７６）に従い測定した。Next, the tensile strengths of the A paper, B paper and bagasse paper were measured according to JIS P8113 (1976).
【００４３】原料バガスからの繊維の収率を測定した。
それぞれの値をＡ紙／バガス紙の相対値及びＢ紙／バガ
ス紙の相対値として表１に示した。The yield of fibers from the raw bagasse was measured.
The respective values are shown in Table 1 as a relative value of A paper / bagasse paper and a relative value of B paper / bagasse paper.
【００４４】[0044]
【表１】 表１ 引張強度（裂断長）の比 収率の比 Ａ紙／バガス紙 １．９２ １．０５ Ｂ紙／バガス紙 ２．１５ １．１０ これらの値から本発明のバガス繊維−バクテリアセルロ
ース複合体から得た紙は、バガス紙と比較して引張強度
が顕著に増強されており、繊維収率も優れていた。Table 1 Table 1 Ratio of tensile strength (breaking length) Ratio of yield A paper / bagasse paper 1.92 1.05 B paper / bagasse paper 2.15 1.10 From these values, the bagasse fiber of the present invention -The paper obtained from the bacterial cellulose composite had a significantly enhanced tensile strength compared to bagasse paper and an excellent fiber yield.
【００４５】また、本発明により得られたバガス繊維−
バクテリアセルロース複合から得た紙の色調、インク印
刷特性及び手触りなどの紙質については、通常の木材由
来の紙と比して何の遜色もなかった。The bagasse fiber obtained according to the present invention
Regarding the paper quality obtained from the bacterial cellulose composite, the color tone, ink printing characteristics, and hand quality were comparable to those of ordinary wood-derived paper.
【００４６】[0046]
【発明の効果】非木材繊維を原料として高強度の紙を収
率良く得られる。EFFECTS OF THE INVENTION High-strength paper can be obtained in good yield from non-wood fibers.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｃ１２Ｒ 1:02） (Ｃ１２Ｐ 19/04 Ｃ１２Ｒ 1:41） (Ｃ１２Ｐ 19/04 Ｃ１２Ｒ 1:38） (Ｃ１２Ｐ 19/04 Ｃ１２Ｒ 1:025） (Ｃ１２Ｐ 19/04 Ｃ１２Ｒ 1:05） (Ｃ１２Ｐ 19/04 Ｃ１２Ｒ 1:065） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification code Internal reference number FI Technical display area C12R 1:02) (C12P 19/04 C12R 1:41) (C12P 19/04 C12R 1:38) (C12P 19/04 C12R 1: 025) (C12P 19/04 C12R 1:05) (C12P 19/04 C12R 1: 065)
Claims (8)
能な炭素源及び／又は窒素源を含有する植物由来のセル
ロース主体組成物中にセルロース生産性微生物を接種
し、培養することを特徴とする繊維−バクテリアセルロ
ース複合体の製造方法。1. A plant-derived cellulose-based composition containing a carbon source and / or a nitrogen source that can be assimilated by a fiber and a cellulose-producing microorganism is inoculated with the cellulose-producing microorganism and cultured. A method for producing a fiber-bacterial cellulose composite.
ａｃｔｅｒ属、Ａｇｒｏｂａｃｔｅｒｉｕｍ属、Ｒｈｉ
ｚｏｂｉｕｍ属、Ｓａｒｃｉｎａ属、Ｐｓｅｕｄｏｍｏ
ｎａｓ属、Ａｃｈｒｏｍｏｂａｃｔｅｒ属、Ａｌｃａｌ
ｉｇｅｎｅｓ属、Ａｅｒｏｂａｃｔｅｒ属、Ａｚｏｔｏ
ｂａｃｔｅｒ属もしくはＺｏｏｇｌｅａ属に属する細菌
又は藻類から選択される一種又はそれ以上の微生物であ
る請求項１の方法。2. The cellulose-producing microorganism is Acetob.
genus Acter, genus Agrobacterium, Rhi
genus zobium, genus Sarcina, Pseudomo
genus nas, genus Achromobacter, Alcal
Igenes, Aerobacterium, Azoto
The method according to claim 1, which is one or more microorganisms selected from bacteria or algae belonging to the genus Bacter or the genus Zooglea.
ａｃｔｅｒ属に属する細菌である請求項２の方法。3. The cellulose-producing microorganism is Acetob.
The method according to claim 2, which is a bacterium belonging to the genus Acter.
１株（ＦＥＲＭ ＢＰ−４５４５）である請求項３の方
法。4. The cellulose-producing microorganism is BPR200.
The method according to claim 3, which is one strain (FERM BP-4545).
混合物である請求項１〜４のいずれかの方法。5. The method according to claim 1, wherein the fiber is a mixture of cellulose and hemicellulose.
ウキビ搾りかす（バガス）、小麦ふすま、大豆搾りか
す、オカラ、籾がら、茶がら、麦芽搾りかす、コーヒー
豆搾りかす、ビート（サトウダイコン）搾りかす、果実
搾りかす、野菜皮、果実皮、及びセルラーゼ処理で一部
糖化したオガクズ、古紙から成る群から選択された少な
くとも１種である請求項１〜５のいずれかの方法。6. A plant-derived cellulose-based composition comprising sugarcane pomace (bagasse), wheat bran, soybean pomace, okara, chaff, tea powder, malt pomace, coffee bean pomace, beet (sugar radish) pomace. The method according to any one of claims 1 to 5, which is at least one selected from the group consisting of fruit pomace, vegetable skin, fruit skin, sawdust partially saccharified by cellulase treatment, and waste paper.
得られ得る繊維−バクテリアセルロース複合体。7. A fiber-bacterial cellulose composite obtainable by the method according to claim 1.
紙。8. A paper comprising a stock containing the composite of claim 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2672894A JP2798882B2 (en) | 1994-02-24 | 1994-02-24 | Method for producing fiber-bacterial cellulose composite and composite obtainable by the method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2672894A JP2798882B2 (en) | 1994-02-24 | 1994-02-24 | Method for producing fiber-bacterial cellulose composite and composite obtainable by the method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH07238488A true JPH07238488A (en) | 1995-09-12 |
| JP2798882B2 JP2798882B2 (en) | 1998-09-17 |
Family
ID=12201388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2672894A Expired - Lifetime JP2798882B2 (en) | 1994-02-24 | 1994-02-24 | Method for producing fiber-bacterial cellulose composite and composite obtainable by the method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2798882B2 (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000020677A1 (en) * | 1998-10-08 | 2000-04-13 | Battelle Memorial Institute | Delignified soybean hull fiber and methods of making and using |
| KR20020080802A (en) * | 2001-04-17 | 2002-10-26 | 변유량 | Method for Manufacturing Microbial Cellulose Employing Soybean Processed Product |
| EP1469122A3 (en) * | 2003-03-24 | 2007-10-03 | Yuen Foong Yu Paper MFG Company, Limited | Non-woody fiber mulching mat and production method thereof |
| EP1471181A3 (en) * | 2003-03-25 | 2007-10-03 | Yuen Foong Yu Paper MFG Company, Limited | Biopulp for non-woody fiber plants and biopulping method thereof |
| WO2008020187A1 (en) * | 2006-08-16 | 2008-02-21 | Imperial Innovations Limited | Material comprising microbially synthesized cellulose associated with a support like a polymer and/or fibre |
| US7452445B2 (en) | 2003-02-26 | 2008-11-18 | Yeun Foong Yu Paper Mfg Co. Ltd. | Non-woody fiber mulching mat and production method thereof |
| JP2010004784A (en) * | 2008-06-25 | 2010-01-14 | Tokai Senko Kk | Method for producing bacterial cellulose, composite structure and defibrated bacterial cellulose |
| WO2011012934A2 (en) | 2009-07-31 | 2011-02-03 | Universidade De Aveiro | Aqueous coating compositions for use in surface treatment of cellulosic substrates |
| JP2013027344A (en) * | 2011-07-28 | 2013-02-07 | Nikken Sohonsha Corp | Culture method of algae and algae obtained by the culture method, and soil conditioner using medium |
| US9790461B2 (en) | 2012-12-14 | 2017-10-17 | Denso Corporation | Culture method and culture system for microalgae |
| WO2018136763A1 (en) | 2017-01-19 | 2018-07-26 | Javacube, Inc. | Cellulosic composition containing coffee parchment cellulose and uses thereof |
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1994
- 1994-02-24 JP JP2672894A patent/JP2798882B2/en not_active Expired - Lifetime
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000020677A1 (en) * | 1998-10-08 | 2000-04-13 | Battelle Memorial Institute | Delignified soybean hull fiber and methods of making and using |
| KR20020080802A (en) * | 2001-04-17 | 2002-10-26 | 변유량 | Method for Manufacturing Microbial Cellulose Employing Soybean Processed Product |
| US7452445B2 (en) | 2003-02-26 | 2008-11-18 | Yeun Foong Yu Paper Mfg Co. Ltd. | Non-woody fiber mulching mat and production method thereof |
| EP1469122A3 (en) * | 2003-03-24 | 2007-10-03 | Yuen Foong Yu Paper MFG Company, Limited | Non-woody fiber mulching mat and production method thereof |
| EP1471181A3 (en) * | 2003-03-25 | 2007-10-03 | Yuen Foong Yu Paper MFG Company, Limited | Biopulp for non-woody fiber plants and biopulping method thereof |
| WO2008020187A1 (en) * | 2006-08-16 | 2008-02-21 | Imperial Innovations Limited | Material comprising microbially synthesized cellulose associated with a support like a polymer and/or fibre |
| JP2010004784A (en) * | 2008-06-25 | 2010-01-14 | Tokai Senko Kk | Method for producing bacterial cellulose, composite structure and defibrated bacterial cellulose |
| WO2011012934A2 (en) | 2009-07-31 | 2011-02-03 | Universidade De Aveiro | Aqueous coating compositions for use in surface treatment of cellulosic substrates |
| JP2013027344A (en) * | 2011-07-28 | 2013-02-07 | Nikken Sohonsha Corp | Culture method of algae and algae obtained by the culture method, and soil conditioner using medium |
| US9790461B2 (en) | 2012-12-14 | 2017-10-17 | Denso Corporation | Culture method and culture system for microalgae |
| WO2018136763A1 (en) | 2017-01-19 | 2018-07-26 | Javacube, Inc. | Cellulosic composition containing coffee parchment cellulose and uses thereof |
| CN113974073A (en) * | 2021-09-27 | 2022-01-28 | 哈尔滨商业大学 | Edible vegetable packaging paper based on vegetable-bean dreg mixture and preparation method thereof |
| CN113974073B (en) * | 2021-09-27 | 2024-04-26 | 哈尔滨商业大学 | Vegetable-bean dreg mixed-base edible vegetable packaging paper and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2798882B2 (en) | 1998-09-17 |
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