JPH06506348A - キシラナーゼ、これに対応する組み換えdna配列、キシラナーゼ含有剤、及びこの剤の使用 - Google Patents
キシラナーゼ、これに対応する組み換えdna配列、キシラナーゼ含有剤、及びこの剤の使用Info
- Publication number
- JPH06506348A JPH06506348A JP4507628A JP50762892A JPH06506348A JP H06506348 A JPH06506348 A JP H06506348A JP 4507628 A JP4507628 A JP 4507628A JP 50762892 A JP50762892 A JP 50762892A JP H06506348 A JPH06506348 A JP H06506348A
- Authority
- JP
- Japan
- Prior art keywords
- xylanase
- array
- aspergillus
- sequence
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Paper (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Claims (22)
- 1.以下の部分的アミノ酸配列: 1【配列があります】,(配列番号1)2【配列があります】,(配列番号2) 3【配列があります】,(配列 番号3) 4【配列があります】,(配列番号 4) 5【配列があります】,(配列番号5)、及び、 6【配列があります】 ,(配列番号6) 又は、これに少なくとも80%、好ましくは少なくとも90%の相同性をもつ部 分的アミノ酸配列をもっているキシラナーゼ。
- 2.上記のキシラナーゼが、フミコーラ・インソレンス(Humicolain solens),DSM1800かり得られた精製キシラナーゼに対して生じた 抗体と免疫反応性がある、請求項1に記載のキシラナーゼ。
- 3.前記のキシラナーゼが、7.5〜9.5の、好ましくは8.0〜8.5の等 電点をもつ、請求項1〜2に記載のキシラナーゼ。
- 4.前記のキシラナーゼが、330EXU/mg蛋白を超える、好ましくは40 0EXU/mg蛋白を超える比活性を示す、請求項1〜3に記載のキシラナーゼ 。
- 5.請求項1〜4に記載のキシラナーゼをコードすることを特徴とする組み換え DNA配列。
- 6.以下の部分的DNA配列: 【配列があります】(=配列番号7) を含んで成る、請求項5に記載の組み換えDNA配列。
- 7.a)pHD450中のフミコーラ・インソレンス(Humicola in solens)のキシラナーゼDNA挿入物 b)a)のDNA挿入物に含まれる成熟キシラナーゼのためのコード領域にハイ ブリダイズし、そして、キシラナーゼ活性をもつポリペプチドのための構造遺伝 子を含んで成り、そして場合により、プロモーター、シグナル若しくはリーダー ・ペプチドのためのコード領域、及び/又は転写ターミネーターを含んで成るD NA配列 c)比較的ストリンジェントな条件(1.0xSSC.0.1%SDS.65℃ )(T.Mantis.A laboratory Manual(CSH)を 参照した)の下で、請求項6に示す配列にハイブリダイズするのに十分な相同性 をもつDNA配列 d)a)、b)若しくはc)に定義したDNA配列の誘導体、又は、e)成熟キ シラナーゼ又はそれらのシグナル・ペプチド若しくはリーダー・ペプチドをコー ドし、そしてa)、b)若しくはc)のDNA配列に関してその遺伝子コードの 意味の範囲内で縮重されているDNA配列 から選ばれたDNA配列を含んで成る、請求項5又は6に記載の組み換えDNA 配列。
- 8.請求項5〜7に記載の組み換えDNA配列を含んで成るベクター。
- 9.前記のプロモーターが、アスペルギルス・オリザエ(Aspergillu s oryzae)のタカアミラーゼ(takaamylase)のプロモータ ーであり、そして/又は前記のキシラナーゼ遺伝子が、EC3−2から単離され ており、そして/又は前記のターミネーターが、アスペルギルスオリサエ(As pergillus oryzae)のAMGターミネーターであり、好ましく はpHD450である、請求項8に記載のベクター。
- 10.請求項8又は9に記載のベクターを含んで成る形質転換宿主。
- 11.上記の形質転換宿主が、アスペルギルス(Aspergillus)株で ある、請求項10に記載の形質転換宿主。
- 12.前記の形質転換宿主が、アスペルギルス・アクレアタス(Aspergi llus aculeatus)、アスペルギルス・ニガー(Aspergil lus niger)、アスペルギルス・オリザエ(Aspergillus oryzae)又はアスペルギルス・アワモリ(Aspergillus aw amori)の種に属する株である、請求項10に記載の形質転換宿主。
- 13.前記の形質転換宿主が、その非形質転換条件においては、キシラナーゼを 生産しないか、又は有意でない量のみのキシラナーゼを生産する微生物、好まし くはバチルス・エスピー(Bacillus sp.)、大腸菌(E.coli )又はサッカロミセス・セレビシエ(S.cerevisiae)である、請求 項10に記載の形質転換宿主。
- 14.請求項10〜13に記載の形質転換宿主によるキシラナーゼの製造方法。
- 15.請求項14に記載の方法により製造されるキシラナーゼ。
- 16.好ましくは非−発埃性粒状物、安定化液体又は保護された酵素の形態であ って、このことにより、キシラナーゼが全酵素蛋白の少なくとも10%、好まし くは少なくとも30%を占める、請求項1〜14又は15のいずれか1項に記載 のキシラナーゼを含む剤。
- 17.キシラナーゼ活性とセルラーゼ活性との比が、キシラナーゼ活性(EXU /g)とセルラーゼ活性(ECU/g)との比として表されるものとして、10 を超える、好ましくは30超える、最も好ましくは100を超える値をもつ、請 求項16に記載の剤。
- 18.少なくとも10EXU/mg酵素蛋白の、好ましくは少なくとも100E XU/mg酵素蛋白の、より好ましくは少なくとも300EXU/mg酵素蛋白 の、キシラナーゼ活性を含む、請求項16又は17に記載の剤。
- 19.前記の剤がキシラン分解にために使用されることを特徴とする、請求項1 6〜18に記載の剤の使用。
- 20.上記の使用が、漂白の前又は一部として、好ましくは7を超えるpH値に て、化学パルプ又はリサイクル紙パルプに関することを特徴とする、請求項19 に記載の使用。
- 21.前記の使用が、小麦粉を含むパンの製造に関することを特徴とする、請求 項19に記載の使用。
- 22.前記の使用が、家畜飼料に関することを特徴とする、請求項19に記載の 使用。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BE91610027.4 | 1991-04-02 | ||
EP91610027 | 1991-04-02 | ||
PCT/DK1992/000099 WO1992017573A1 (en) | 1991-04-02 | 1992-03-27 | Xylanase, corresponding recombinant dna sequence, xylanase containing agent, and use of the agent |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH06506348A true JPH06506348A (ja) | 1994-07-21 |
JP3483880B2 JP3483880B2 (ja) | 2004-01-06 |
Family
ID=8208770
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50762892A Expired - Lifetime JP3483880B2 (ja) | 1991-04-02 | 1992-03-27 | キシラナーゼ、これに対応する組み換えdna配列、キシラナーゼ含有剤、及びこの剤の使用 |
Country Status (13)
Country | Link |
---|---|
US (1) | US5610048A (ja) |
EP (2) | EP0507723A1 (ja) |
JP (1) | JP3483880B2 (ja) |
KR (1) | KR100234888B1 (ja) |
AT (1) | ATE176498T1 (ja) |
CA (1) | CA2106484A1 (ja) |
DE (1) | DE69228378T2 (ja) |
DK (1) | DK0579672T3 (ja) |
ES (1) | ES2130173T3 (ja) |
FI (1) | FI116795B (ja) |
NO (1) | NO933525L (ja) |
NZ (1) | NZ242201A (ja) |
WO (1) | WO1992017573A1 (ja) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5837515A (en) * | 1990-05-16 | 1998-11-17 | Alko-Yhtiot Oy | Enzyme preparations and methods for their production |
ATE412048T1 (de) * | 1993-03-10 | 2008-11-15 | Novozymes As | Enzyme mit xylanaseaktivität aus aspergillus aculeatus |
JP2807612B2 (ja) * | 1993-03-12 | 1998-10-08 | ノボ ノルディスク アクティーゼルスカブ | 新規キシラナーゼ、その製造法、該キシラナーゼによるパルプ処理方法及びキシロオリゴ糖の製造法 |
CA2185101A1 (en) | 1994-03-08 | 1995-09-14 | Martin Schulein | Novel alkaline cellulases |
GB9406317D0 (en) * | 1994-03-30 | 1994-05-25 | Finnfeeds Int Ltd | Use of an enzyme for assisting an animal to digest protein |
BE1008751A3 (fr) | 1994-07-26 | 1996-07-02 | Solvay | Xylanase, microorganismes la produisant, molecules d'adn, procedes de preparation de cette xylanase et utilisations de celle-ci. |
GB9416841D0 (en) * | 1994-08-19 | 1994-10-12 | Finnfeeds Int Ltd | An enzyme feed additive and animal feed including it |
EP0805856B2 (en) * | 1995-01-26 | 2009-04-01 | Novozymes A/S | Animal feed additives comprising xylanase |
AU715423B2 (en) | 1995-03-17 | 2000-02-03 | Novozymes A/S | Novel endoglucanases |
AU5333396A (en) * | 1995-04-11 | 1996-10-30 | Novo Nordisk A/S | Bread-improving additive comprising a xylanolytic enzyme |
CA2232709C (en) | 1995-09-22 | 2002-11-19 | Terragen Diversity Inc. | Method for isolating xylanase gene sequences from soil dna, compositions useful in such method and compositions obtained thereby |
CA2234998A1 (en) * | 1995-10-17 | 1997-04-24 | Institut National De La Recherche Agronomique (Inra) | Xylanase, oligonucleotidic sequence encoding it and its uses |
AU1437597A (en) * | 1996-01-22 | 1997-08-20 | Novo Nordisk A/S | An enzyme with xylanase activity |
AU7235901A (en) | 2000-07-06 | 2002-01-21 | Novozymes As | Method of preparing a dough or a baked product made from a dough, with addition of lipolytic enzymes |
ES2394481T3 (es) | 2000-09-21 | 2013-02-01 | Basf Se | Xilanasa de talaromyces |
US7273738B2 (en) * | 2002-10-01 | 2007-09-25 | Novozymes A/S | Family GH-61 polypeptides |
EP2270139B1 (en) | 2003-05-09 | 2016-07-27 | Novozymes A/S | Variant lipolytic enzymes |
EP2322630B1 (en) | 2004-02-12 | 2016-11-16 | Novozymes Inc. | Polypeptides having xylanase activity and polynucleotides encoding same |
WO2006031554A2 (en) | 2004-09-10 | 2006-03-23 | Novozymes North America, Inc. | Methods for preventing, removing, reducing, or disrupting biofilm |
AU2008207001B2 (en) | 2007-01-16 | 2013-05-02 | Puratos N.V. | Bread with increased arabinoxylo-oligosaccharide content |
BRPI0806921A2 (pt) | 2007-01-18 | 2014-04-29 | Danisco Us Inc Genecor Division | Endoglicanase ii modificada e métodos de uso |
BRPI0808513A2 (pt) | 2007-03-09 | 2014-08-19 | Danisco Us Inc Genencor Div | Variantes de alfa-amilase de espécies de bacillus alcalifílico, composições compreendendo variantes de alfa-amilase e métodos de uso |
US8916369B2 (en) | 2007-03-14 | 2014-12-23 | Danisco Us Inc. | Trichoderma reesei α-amylase is a maltogenic enzyme |
CN101918545A (zh) | 2008-01-02 | 2010-12-15 | 丹尼斯科美国公司 | 应用嗜糖假单胞菌g4-淀粉酶和其变体获得乙醇的无葡糖淀粉酶的方法 |
JP5702714B2 (ja) | 2008-04-30 | 2015-04-15 | ダニスコ・ユーエス・インク | 新規キメラアルファアミラーゼ変異体 |
ES2538406T3 (es) | 2008-06-06 | 2015-06-19 | Danisco Us Inc. | Alfa-amilasas variantes de Bacillus subtilis y sus métodos de uso |
JP5599113B2 (ja) | 2008-06-06 | 2014-10-01 | ダニスコ・ユーエス・インク | 糖化酵素組成物及びその糖化方法 |
US9040278B2 (en) | 2008-06-06 | 2015-05-26 | Danisco Us Inc. | Production of glucose from starch using alpha-amylases from Bacillus subtilis |
WO2010081869A1 (en) | 2009-01-16 | 2010-07-22 | Danisco A/S | Enzymatic generation of functional lipids from cereals or cereal bi-streams |
WO2010081870A1 (en) | 2009-01-16 | 2010-07-22 | Danisco A/S | Enzymatic generation of oligasaccharides from cereals or cereal bi-streams |
EP2393925B1 (en) | 2009-02-06 | 2014-10-08 | University of Chile | Protein and DNA sequence encoding a cold adapted xylanase |
BRPI1012562B1 (pt) | 2009-03-31 | 2020-10-27 | Dupont Nutrition Biosciences Aps | método para reduzir a cor e/ou sabor desagradável e/ou desenvolvimento de mau cheiro em um farelo de cereal solubilizado |
DK3473711T3 (da) | 2009-05-19 | 2021-01-04 | Dupont Nutrition Biosci Aps | Amylasepolypeptider |
FR2959515A1 (fr) | 2010-05-03 | 2011-11-04 | Puratos | Compositions riches en oligosaccharides d'arabinoxylane |
RU2457246C1 (ru) * | 2011-03-21 | 2012-07-27 | Государственное научное учреждение Всероссийский научно-исследовательский институт пищевой биотехнологии Российской академии сельскохозяйственных наук | Рекомбинантный штамм мицелиального гриба aspergillus awamori - продуцент комплекса ферментов глюкоамилазы и ксиланазы |
US8759041B1 (en) * | 2013-02-12 | 2014-06-24 | Novozymes Inc. | Polypeptides having xylanase activity and polynucleotides encoding same |
WO2015150372A1 (en) | 2014-04-01 | 2015-10-08 | Dupont Nutrition Biosciences Aps | Method for increasing crude palm oil yields |
WO2016097266A1 (en) | 2014-12-19 | 2016-06-23 | Dupont Nutrition Biosciences Aps | Method of improving palm oil yields from palm fruit or palm fruit liquid |
WO2016140960A1 (en) | 2015-03-04 | 2016-09-09 | Dupont Nutrition Biosciences Aps | Cereal grain processing |
GB201522603D0 (en) | 2015-12-22 | 2016-02-03 | Dupont Nutrition Biosci Aps | Composition |
CN112522240A (zh) * | 2019-09-18 | 2021-03-19 | 重庆工商大学 | 一种纯化木聚糖酶的方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4652639A (en) * | 1982-05-06 | 1987-03-24 | Amgen | Manufacture and expression of structural genes |
EP0305216B1 (en) * | 1987-08-28 | 1995-08-02 | Novo Nordisk A/S | Recombinant Humicola lipase and process for the production of recombinant humicola lipases |
US5116746A (en) * | 1988-03-04 | 1992-05-26 | Institut Armand Frappier | Cellulase-free endo-xylanase enzyme of use in pulp delignification |
AU2542888A (en) * | 1988-07-27 | 1990-02-19 | Iowa State University Research Foundation Inc. | Low molecular weight xylanase glycoprotein |
WO1991002791A1 (en) * | 1989-08-14 | 1991-03-07 | Cultor Oy | Enzymatic bleaching of pulp |
DK420289D0 (da) * | 1989-08-25 | 1989-08-25 | Novo Nordisk As | Fremgangsmaade til behandling af lignocellulosepulp |
DE4017150A1 (de) * | 1990-05-28 | 1991-12-05 | Henkel Kgaa | Verfahren zur herstellung eines backaktiven pentosanase-praeparates |
-
1992
- 1992-03-27 KR KR1019930702943A patent/KR100234888B1/ko not_active IP Right Cessation
- 1992-03-27 DE DE69228378T patent/DE69228378T2/de not_active Expired - Lifetime
- 1992-03-27 AT AT92908166T patent/ATE176498T1/de active
- 1992-03-27 DK DK92908166T patent/DK0579672T3/da active
- 1992-03-27 JP JP50762892A patent/JP3483880B2/ja not_active Expired - Lifetime
- 1992-03-27 WO PCT/DK1992/000099 patent/WO1992017573A1/en active IP Right Grant
- 1992-03-27 EP EP92610022A patent/EP0507723A1/en active Pending
- 1992-03-27 ES ES92908166T patent/ES2130173T3/es not_active Expired - Lifetime
- 1992-03-27 CA CA002106484A patent/CA2106484A1/en not_active Abandoned
- 1992-03-27 EP EP92908166A patent/EP0579672B1/en not_active Expired - Lifetime
- 1992-03-27 US US08/119,169 patent/US5610048A/en not_active Expired - Lifetime
- 1992-04-02 NZ NZ242201A patent/NZ242201A/xx unknown
-
1993
- 1993-10-01 FI FI934330A patent/FI116795B/fi not_active IP Right Cessation
- 1993-10-01 NO NO93933525A patent/NO933525L/no not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
WO1992017573A1 (en) | 1992-10-15 |
EP0579672B1 (en) | 1999-02-03 |
CA2106484A1 (en) | 1992-10-03 |
FI934330A0 (fi) | 1993-10-01 |
ATE176498T1 (de) | 1999-02-15 |
NO933525D0 (no) | 1993-10-01 |
ES2130173T3 (es) | 1999-07-01 |
EP0579672A1 (en) | 1994-01-26 |
DE69228378T2 (de) | 1999-08-26 |
DK0579672T3 (da) | 1999-09-20 |
KR100234888B1 (ko) | 1999-12-15 |
DE69228378D1 (de) | 1999-03-18 |
JP3483880B2 (ja) | 2004-01-06 |
NZ242201A (en) | 1993-05-26 |
FI116795B (fi) | 2006-02-28 |
NO933525L (no) | 1993-10-01 |
EP0507723A1 (en) | 1992-10-07 |
US5610048A (en) | 1997-03-11 |
FI934330A (fi) | 1993-10-01 |
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