JPH06225723A - Food additive - Google Patents
Food additiveInfo
- Publication number
- JPH06225723A JPH06225723A JP5017229A JP1722993A JPH06225723A JP H06225723 A JPH06225723 A JP H06225723A JP 5017229 A JP5017229 A JP 5017229A JP 1722993 A JP1722993 A JP 1722993A JP H06225723 A JPH06225723 A JP H06225723A
- Authority
- JP
- Japan
- Prior art keywords
- leaf
- flower
- food additive
- ace
- freeze
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は食品添加剤に関し、更に
詳細には血圧降下機能を有する食品添加剤に関する。FIELD OF THE INVENTION The present invention relates to a food additive, and more particularly to a food additive having a blood pressure lowering function.
【０００２】[0002]
【従来の技術】人間の体内には、アンジオテイシン１(A
sp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu）なるデカペ
プチドが存在していることが知られている。このペプチ
ドはこのままでは不活性であるが、アンジオテンシン変
換酵素（以下ＡＣＥと略す。ＡＣＥ：Angiotensin Conv
erting Enzyme ) によって末端のペプチド（His-Leu ）
が切断されて、血圧上昇作用のあるアンジオテイシン２
(Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) を生じる。かかる
アンジオテイシン２は、血管収縮やアルドステロンの分
泌等を促して血圧上昇をもたらす。従って、アンジオテ
イシン１を活性化する酵素であるＡＣＥの活性を阻害す
ることができれば、血圧上昇の抑制が可能である。現実
にこの観点から阻害物質の検索が行われており、医薬品
としてペプチド誘導体などが開発され、実用化されてい
る。2. Description of the Related Art In the human body, angiothecin 1 (A
It is known that a decapeptide called sp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu) exists. This peptide is inactive as it is, but angiotensin converting enzyme (hereinafter abbreviated as ACE. ACE: Angiotensin Conv)
erting Enzyme) for the terminal peptide (His-Leu)
Is cut off to increase blood pressure Angiotheisin 2
(Asp-Arg-Val-Tyr-Ile-His-Pro-Phe). Such angiotheisin 2 promotes vasoconstriction, secretion of aldosterone, and the like to raise blood pressure. Therefore, if the activity of ACE, which is an enzyme that activates angiotheisin 1, can be inhibited, the increase in blood pressure can be suppressed. In fact, the inhibitory substances are searched from this viewpoint, and peptide derivatives and the like have been developed and put into practical use as pharmaceuticals.
【０００３】[0003]
【発明が解決しようとする課題】ところで、このＡＣＥ
活性阻害物質の多くは、合成品であるため、食品添加剤
として使用できない。このため、カツオブシや大豆等に
含有される天然タンパク質を原料として、酵素分解によ
り同様の機能を有する物質を生成することが研究されて
いる。但し、これらの酵素分解反応では、反応条件に大
きく影響され、更にＡＣＥ活性阻害物質の分離精製も難
しいため、充分なＡＣＥ活性阻害能を有する食品添加剤
を得ることができなかった。そこで、本発明の目的は、
天然物から、安定して且つ充分にＡＣＥ活性機能を阻害
できる食品添加剤を提供することにある。By the way, this ACE
Many of the activity inhibitors are synthetic products and cannot be used as food additives. Therefore, it has been studied to produce a substance having a similar function by enzymatic decomposition using natural protein contained in beetle, soybean, etc. as a raw material. However, in these enzymatic decomposition reactions, it was difficult to obtain a food additive having a sufficient ACE activity inhibitory ability because the reaction conditions were greatly affected and the separation and purification of the ACE activity inhibitory substance were difficult. Therefore, the purpose of the present invention is to
It is to provide a food additive that can stably and sufficiently inhibit the ACE activity function from natural products.
【０００４】[0004]
【課題を解決するための手段】本発明者等は、前記目的
を達成すべく検討を重ねた結果、アブラナ花等を凍結乾
燥後粉砕し、その粉砕物を水抽出して得られた抽出物
が、高度のＡＣＥ阻害能を有することを見出し本発明に
到達した。即ち、本発明は、アブラナ花、イタリアンパ
スレー葉、ウド葉、カキドウシ葉、カラハナソウ葉、ク
サボケ花、クズ葉花、シシウド花実、シュプレット葉、
スイバ葉、デイル葉、ニガナ全草、ニリン草全草、ヒメ
ジオン花、ペパーミント葉、マスタード葉、ワレモコウ
葉、矢車草花葉、ヤマフジ花から成る群より選択した少
なくとも１種を凍結乾燥後粉砕し、その粉砕物を水抽出
して得られた抽出物を含むことを特徴としている。かか
る構成の本発明において、ＡＣＥ活性阻害率が５０％以
上であると好適である。Means for Solving the Problems The inventors of the present invention have conducted extensive studies to achieve the above-mentioned object, and as a result, freeze-dried rapeseed flowers and the like, pulverized them, and extracted the pulverized product with water. Have a high degree of ACE inhibitory ability, and have arrived at the present invention. That is, the present invention is a rapeseed flower, an Italian pastry leaf, an udo leaf, an oyster leaf, an arachis leaf, a ragweed flower, a kudzu leaf flower, a citrus fruit, a splet leaf,
Freeze-drying and pulverizing at least one selected from the group consisting of sorrel leaves, dale leaves, whole plants of Nigana, whole plants of Nirin grass, Himedion flowers, peppermint leaves, mustard leaves, Valerian leaves, Yarrow grass flowers, and Yamafuji flowers It is characterized in that it contains an extract obtained by extracting the ground product with water. In the present invention having such a configuration, it is preferable that the ACE activity inhibition rate is 50% or more.
【０００５】[0005]
【作用】本発明において提供される食品添加剤は、酵素
反応等を何等施しておらず、温度制御やｐＨ調整等の反
応系の制御や、カラム精製などの煩雑な操作を必要とし
ないため、容易に得ることができ、しかも安価である。
また、なんら化学的処理をしていないためそのまま食品
添加物として安全に使用することができる。The food additive provided in the present invention does not undergo any enzymatic reaction or the like and does not require control of the reaction system such as temperature control or pH adjustment, or complicated operations such as column purification. It is easy to obtain and inexpensive.
Moreover, since it is not chemically treated, it can be safely used as it is as a food additive.
【０００６】[0006]
【発明の概要】本発明の食品添加剤には、アブラナ花、
イタリアンパスレー葉、ウド葉、カキドウシ葉、カラハ
ナソウ葉、クサボケ花、クズ葉花、シシウド花実、シュ
プレット葉、スイバ葉、デイル葉、ニガナ全草、ニリン
草全草、ヒメジオン花、ペパーミント葉、マスタード
葉、ワレモコウ葉、矢車草花葉、ヤマフジ花から成る群
より選択した少なくとも１種を凍結乾燥後粉砕し、その
粉砕物を水抽出して得られた抽出物を用いることが大切
である。前記植物片は、凍結乾燥処理して乾燥後重量を
自重量の１／３重量部以下に乾燥した後、粉砕して抽出
に供するのが好ましい。乾燥には凍結乾燥の他、熱風に
よる加熱乾燥、自然乾燥によることが考えられるが、後
二者の乾燥植物片からの抽出物では、充分なＡＣＥ活性
阻害能が得られなかった。理由は定かでないが、凍結乾
燥した場合の抽出液が充分なＡＣＥ活性阻害能を有す
る。また、抽出用の溶媒は水を用いる。かかる水は蒸留
水であってもよく、熱水または冷水でもよい。SUMMARY OF THE INVENTION Food additives of the present invention include rapeseed flowers,
Italian pastry leaf, udo leaf, oyster leaf, arachis sinensis leaf, ragweed flower, kudzu leaf flower, cypress flower seed, splet leaf, sorrel leaf, dale leaf, nigana whole plant, nirin grass whole plant, himeedion flower, peppermint leaf, mustard leaf It is important to use the extract obtained by freeze-drying and pulverizing at least one selected from the group consisting of oleander leaf, cornflower leaf, and yamafuji flower, and pulverizing the pulverized product. It is preferable that the plant pieces are freeze-dried and then dried to a weight of 1/3 part by weight or less of the own weight, and then crushed and then subjected to extraction. The drying may be lyophilization, heat drying with hot air, or natural drying. However, the extract from dried plant pieces of the latter two did not have sufficient ability to inhibit ACE activity. Although the reason is not clear, the extract obtained by freeze-drying has sufficient ability to inhibit ACE activity. Water is used as the extraction solvent. Such water may be distilled water, hot water or cold water.
【０００７】これら粉末状の植物片の水抽出は、任意の
方法で行うことができる。例えば攪拌機付きの容器に粉
末状の植物片と所定量の水とを加え、両者を攪拌しつつ
抽出することができる。あるいは粉末状の植物片を充填
したカラムを立設し、カラムの上端から所定量の水を流
下してもよい。この場合、カラムの下端から流出した流
出液を再度カラムの上端より滴下するのが好ましい。抽
出に際して条件は特に制限されないが、室温から５０℃
程度の温度で１０〜６０分間行うのが好ましい。抽出に
際して、粉末状の植物片と溶媒の比率は、粉末状の植物
片１重量部（乾燥重量）あたり水４００重量部以下でよ
く、特に水１００重量部以下で、１重量部の粉末状の植
物片から抽出して得られた抽出液は、高度のＡＣＥ活性
阻害能を有している。抽出完了後、濾過あるいは円心分
離等で固液分離して、固形物を除去し、得られた液体を
必要に応じて濃縮し抽出液とする。濃縮した抽出液をさ
らに減圧濃縮して固形物としても良い。得られた液状あ
るいは固形状の抽出物は、原料が食用植物と水だけであ
り、且つ、触媒等の添加物を添加することなく抽出した
ものであるため、そのまま食品添加剤として使用するこ
とができる。尚、かかる食品添加剤中には食品添加剤と
して許容されている他の添加剤が含有されていてもよ
い。添加する食品の種類には特に限定されない。Water extraction of these powdery plant pieces can be carried out by any method. For example, powdery plant pieces and a predetermined amount of water can be added to a container equipped with a stirrer, and both can be extracted with stirring. Alternatively, a column filled with powdery plant pieces may be provided upright and a predetermined amount of water may be flown down from the upper end of the column. In this case, it is preferable to drop the effluent flowing out from the lower end of the column again from the upper end of the column. Conditions for extraction are not particularly limited, but from room temperature to 50 ° C.
It is preferable to carry out at a temperature of about 10 to 60 minutes. In the extraction, the ratio of the powdered plant pieces to the solvent may be 400 parts by weight or less of water per 1 part by weight (dry weight) of the powdered plant pieces, and particularly 100 parts by weight or less of water and 1 part by weight of the powdered plant pieces. The extract obtained by extracting from plant pieces has a high ability to inhibit ACE activity. After the extraction is completed, solid-liquid separation is performed by filtration or centrifuge separation to remove solids, and the obtained liquid is concentrated as necessary to obtain an extract. The concentrated extract may be further concentrated under reduced pressure to give a solid. The obtained liquid or solid extract is a raw material consisting only of edible plants and water, and since it was extracted without adding additives such as catalysts, it can be used as it is as a food additive. it can. It should be noted that the food additive may contain other additives that are acceptable as food additives. The type of food to be added is not particularly limited.
【０００８】[0008]
【実施例】本発明を実施例によって更に詳細に説明する
が、先ず本実施例において使用したＡＣＥ酵素液、ＡＣ
Ｅ活性阻害率の測定法について説明する。 （１）ＡＣＥ酵素液 ウサギ肺のアセトンパウダー１ｇ当たり５０mmolリン酸
緩衝液(pH 8.36)10mlで抽出し（４℃、２４時間）、遠
心分離して上澄みの溶液をＡＣＥ酵素液として用いた。 （２）ＡＣＥ活性阻害率の測定法 ＡＣＥ活性阻害物質が含有される被試験液に、ＡＣＥ酵
素液２．５ｍＵ、基質〔Hippury-His-Leu(C6H5CONHCH2C
O-His-Leu)〕１２．５mmol、ほう酸ナトリウム緩衝液
（pH 8.3）、及び食塩を加え３７℃で２時間反応させ
た。この反応において、ＡＣＥ酵素の作用によって基質
が分解され、馬尿酸 (C6H5CONHCH2CO2H)が生成される。
したがって、ＡＣＥ酵素の活性阻害率は、生成した馬尿
酸を検出することによって測定できる。生成した馬尿酸
量の測定は、馬尿酸を酸性下にて抽出して濃縮した後、
メタノール溶液とし、高速液クロマトグラフィーにて馬
尿酸量を検量した。一方、被試験溶液に代えて蒸留水を
用い、上記と同様にＡＣＥ酵素液、基質、ほう酸ナトリ
ウム緩衝液、及び食塩を加えて反応させ、生成した馬尿
酸量を測定した。ＡＣＥ活性阻害率は、被試験溶液中の
馬尿酸の生成量Ａ１と、被試験溶液の代わりに蒸留水を
用いたときの馬尿酸の生成量Ａ２により以下の式にて算
出した。 ＡＣＥ活性阻害率（％）＝（１−（Ａ２−Ａ１）／Ａ
２）×１００ この値は、ＡＣＥが全く阻害を受けないとき０％、完全
に阻害されたとき１００％となる。ＡＣＥ活性阻害率が
５０％以上のものを合格とした。EXAMPLES The present invention will be described in more detail with reference to Examples. First, the ACE enzyme solution and AC used in this Example.
The method for measuring the E activity inhibition rate will be described. (1) ACE enzyme solution Extracted with 10 ml of 50 mmol phosphate buffer (pH 8.36) per 1 g of acetone powder of rabbit lung (4 ° C., 24 hours), centrifuged, and the supernatant solution was used as the ACE enzyme solution. (2) Method for measuring ACE activity inhibition rate To a test solution containing an ACE activity inhibitor, 2.5 mU of ACE enzyme solution, substrate [Hippury-His-Leu (C 6 H 5 CONHCH 2 C
O-His-Leu)] 12.5 mmol, sodium borate buffer (pH 8.3), and sodium chloride were added and reacted at 37 ° C for 2 hours. In this reaction, the substrate is decomposed by the action of the ACE enzyme, and hippuric acid (C 6 H 5 CONHCH 2 CO 2 H) is produced.
Therefore, the activity inhibition rate of the ACE enzyme can be measured by detecting the produced hippuric acid. The amount of hippuric acid produced was measured by extracting and concentrating hippuric acid under acidic conditions,
Using a methanol solution, the amount of hippuric acid was calibrated by high performance liquid chromatography. On the other hand, distilled water was used in place of the solution to be tested, and an ACE enzyme solution, a substrate, a sodium borate buffer, and salt were added and reacted in the same manner as above, and the amount of hippuric acid produced was measured. The ACE activity inhibition rate was calculated by the following formula from the production amount A1 of hippuric acid in the test solution and the production amount A2 of hippuric acid when distilled water was used instead of the test solution. ACE activity inhibition rate (%) = (1- (A2-A1) / A
2) × 100 This value is 0% when ACE is completely uninhibited and 100% when it is completely inhibited. Those with an ACE activity inhibition rate of 50% or more were accepted.
【０００９】実施例１ 表１に示す各種植物の凍結乾燥粉末を調整し、その水抽
出液のＡＣＥ活性阻害率を測定した。まず、原料の生組
織２０ｇを一晩かけて凍結乾燥した後粉砕した。粉砕後
の保存は、冷凍庫にて行った。この乾燥粉末０．５ｇに
蒸留水４０ｍｌを加え、３０℃、１０分間、１５０ｒｐ
ｍの回転速度の攪拌器にて攪拌抽出した。更に、遠心分
離機によって、１２．０００ｒｐｍの回転速度で、１５
分間遠心分離し、得られた上澄みを原液とした。次い
で、この原液を５倍希釈した液についてＡＣＥ阻害能を
調査した。その調査結果を表１に示す。Example 1 Freeze-dried powders of various plants shown in Table 1 were prepared, and the ACE activity inhibition rate of the water extract thereof was measured. First, 20 g of raw tissue as a raw material was freeze-dried overnight and then pulverized. Storage after crushing was performed in a freezer. Distilled water (40 ml) was added to this dry powder (0.5 g) at 30 ° C. for 10 minutes at 150 rp.
It was stirred and extracted with a stirrer having a rotation speed of m. Furthermore, by using a centrifuge, at a rotation speed of 12.000 rpm,
After centrifuging, the resulting supernatant was used as a stock solution. Next, the ACE inhibitory ability of the stock solution diluted 5-fold was investigated. The survey results are shown in Table 1.
【００１０】[0010]
【表１】 [Table 1]
【００１１】表１から明らかな様に、アブラナ花、イタ
リアンパスレー葉、ウド葉、カキドウシ葉、カラハナソ
ウ葉、クサボケ花、クズ葉花、シシウド花実、シュプレ
ット葉、スイバ葉、デイル葉、ニガナ全草、ニリン草全
草、ヒメジオン花、ペパーミント葉、マスタード葉、ワ
レモコウ葉、矢車草花葉、ヤマフジ花については、ＡＣ
Ｅ活性阻害率は５０％以上であった。これに対し、前記
植物以外のアカザ葉等については、ＡＣＥ活性阻害率は
５０％未満であった。また表２に、生原料を各種の乾燥
法を用いて乾燥して、この乾燥品の水抽出物のＡＣＥ活
性阻害能を調べた結果を示す。加熱乾燥では、オートク
レーブを用いて生植物片１０ｇを７０℃、５時間加熱乾
燥させた。自然乾燥では、生植物片２０ｇを日陰にて２
週間通風乾燥させた。As is clear from Table 1, rapeseed flowers, Italian pastry leaves, udo leaves, oyster cow leaves, arachis sinensis leaves, ragweed flowers, kudzu leaf flowers, citrus fruit seeds, splet leaves, sorrel leaves, dale leaves, and all-in-one plants , Whole herring, Himedion flower, peppermint leaf, mustard leaf, oleander leaf, cornflower leaf, Yamafuji flower, AC
The E activity inhibition rate was 50% or more. On the other hand, the ACE activity inhibition rate was less than 50% with respect to the leaves of the plants other than the above plants. In addition, Table 2 shows the results of examining the raw material dried by various drying methods and examining the ACE activity inhibiting ability of the water extract of this dried product. In the heat drying, 10 g of fresh plant pieces were heat dried at 70 ° C. for 5 hours using an autoclave. In the case of natural drying, 20 g of fresh plant pieces are shaded in 2
It was air-dried for a week.
【００１２】[0012]
【表２】 [Table 2]
【００１３】表２から明らかなように、加熱乾燥、自然
乾燥、生いずれの場合も充分なＡＣＥ活性阻害能が得ら
れず、凍結乾燥による乾燥品の抽出物のみが、充分なＡ
ＣＥ活性阻害能を有することがわかる。As is clear from Table 2, sufficient ACE activity inhibitory ability was not obtained in any of heat drying, natural drying, and raw, and only the extract of the dried product by freeze-drying had sufficient A
It can be seen that it has the ability to inhibit CE activity.
【００１４】[0014]
【発明の効果】本発明の食品添加剤は、アブラナ花等の
特定の植物を凍結乾燥後粉砕し、その粉砕物を水抽出し
て得られた抽出物を用いるので、何等の合成もなされて
おらず、何等の懸念なく食品添加剤として使用すること
ができる。また、本食品添加剤は、良好なＡＣＥ活性阻
害能を有するため、血圧降下機能を有し、本発明の食品
添加剤が添加された食品を継続して摂取することによっ
て、成人病である高血圧症の治療に役立たせることがで
きる。Industrial Applicability The food additive of the present invention uses an extract obtained by freeze-drying a specific plant such as rapeseed flower and pulverizing the pulverized product, and extracting the pulverized product with water. Therefore, it can be used as a food additive without any concern. Further, since the food additive has a good ability to inhibit ACE activity, it has a blood pressure lowering function, and by continuously ingesting the food to which the food additive of the present invention is added, hypertension which is an adult disease Can be used to treat the disease.
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【手続補正書】[Procedure amendment]
【提出日】平成５年１２月１６日[Submission date] December 16, 1993
【手続補正１】[Procedure Amendment 1]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】特許請求の範囲[Name of item to be amended] Claims
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【特許請求の範囲】[Claims]
【手続補正２】[Procedure Amendment 2]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】０００４[Correction target item name] 0004
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【０００４】[0004]
【課題を解決するための手段】本発明者等は、前記目的
を達成すべく検討を重ねた結果、アブラナ花等を凍結乾
燥後粉砕し、その粉砕物を水抽出して得られた抽出物
が、高度のＡＣＥ阻害能を有することを見出し本発明に
到達した。即ち、本発明は、アブラナ花、イタリアンパ
スレー葉、ウド葉、カキドウシ葉、カラハナソウ葉、ク
サボケ花、クズ葉花、シシウド花実、シュプレット葉、
スイバ葉、デイル葉、ニガナ全草、ニリン草全草、ヒメ
ジオン花、ペパーミント葉、マスタード葉、ワレモコウ
葉、矢車草花葉、ヤマフジ花から成る群より選択した少
なくとも１種を凍結乾燥後粉砕し、その粉砕物を水抽出
して得られた抽出物を含むことを特徴としている。かか
る構成の本発明において、粉砕物１重量部あたり水４０
０重量部で抽出したとき、得られた抽出液のＡＣＥ活性
阻害率が５０％以上であることが好適である。 Means for Solving the Problems The inventors of the present invention have conducted extensive studies to achieve the above-mentioned object, and as a result, freeze-dried rapeseed flowers and the like, pulverized them, and extracted the pulverized product with water. Have a high degree of ACE inhibitory ability, and have arrived at the present invention. That is, the present invention is a rapeseed flower, an Italian pastry leaf, an udo leaf, an oyster leaf, an arachis leaf, a ragweed flower, a kudzu leaf flower, a citrus fruit, a splet leaf,
Freeze-drying and pulverizing at least one selected from the group consisting of sorrel leaves, dale leaves, whole plants of Nigana, whole plants of Nirin grass, Himedion flowers, peppermint leaves, mustard leaves, Valerian leaves, Yarrow grass flowers, and Yamafuji flowers It is characterized in that it contains an extract obtained by extracting the ground product with water. In the present invention having such a structure, 40 parts of water is used per 1 part by weight of the pulverized product.
ACE activity of the resulting extract when extracted with 0 parts by weight
It is preferable that the inhibition rate is 50% or more.
Claims (2)
ド葉、カキドウシ葉、カラハナソウ葉、クサボケ花、ク
ズ葉花、シシウド花実、シュプレット葉、スイバ葉、デ
イル葉、ニガナ全草、ニリン草全草、ヒメジオン花、ペ
パーミント葉、マスタード葉、ワレモコウ葉、矢車草花
葉、ヤマフジ花から成る群より選択した少なくとも１種
を凍結乾燥後粉砕し、その粉砕物を水抽出して得られた
抽出物を含み、且つアンジオテイシン変換酵素の存在下
において、アンジオテイシン１からアンジオテイシン２
への変換抑制機能（ＡＣＥ活性阻害機能）を有すること
を特徴とする食品添加剤。1. A canola flower, an Italian pastry leaf, an udo leaf, an oyster leaf, an arachis leaf, a ragweed flower, a kudzu leaf flower, a citrus fruit seed, a splet leaf, a sorrel leaf, a dale leaf, a whole plant of Nigana, a whole plant of Nirinku, Himedione flower, peppermint leaf, mustard leaf, oleander leaf, cornflower leaf, at least one selected from the group consisting of Yamafuji flowers is freeze-dried and then crushed, and the crushed product contains an extract obtained by water extraction, In the presence of angiotheisin converting enzyme, angiotheisin 1 to angiotheisin 2
A food additive characterized by having a function of suppressing conversion to ACE (function of inhibiting ACE activity).
ＣＥ活性阻害率が５０％以上である請求項１記載の食品
添加剤。2. A of a 5-fold dilution of the water extract of the crushed product
The food additive according to claim 1, which has a CE activity inhibition rate of 50% or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5017229A JPH07100016B2 (en) | 1993-02-04 | 1993-02-04 | Food additives |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5017229A JPH07100016B2 (en) | 1993-02-04 | 1993-02-04 | Food additives |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06225723A true JPH06225723A (en) | 1994-08-16 |
| JPH07100016B2 JPH07100016B2 (en) | 1995-11-01 |
Family
ID=11938126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5017229A Expired - Lifetime JPH07100016B2 (en) | 1993-02-04 | 1993-02-04 | Food additives |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07100016B2 (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999057984A1 (en) * | 1998-05-12 | 1999-11-18 | Jungong Xiong | Use of hybrid rumex acetosa l. in production of wheat flour-containing product |
| WO2001001795A1 (en) * | 1999-06-30 | 2001-01-11 | Jungong Xiong | Use of rumex k-1 hybrid rumex acetosa for preparing menu |
| KR100297812B1 (en) * | 1998-07-27 | 2001-11-05 | 한수길 | Foods containing stone extracts with antihypertensive effect |
| JP2001335494A (en) * | 2000-05-29 | 2001-12-04 | Okinawa Shokuryo Kk | Angiotensin-converting enzyme inhibitor |
| US6638540B2 (en) | 1997-05-06 | 2003-10-28 | Universitat Bern | Plant extracts for the treatment of increased bone resorption |
| JP2004115440A (en) * | 2002-09-26 | 2004-04-15 | Pola Chem Ind Inc | Calorigenic sympathetic nerve activator and composition containing the same |
| KR100511515B1 (en) * | 2002-10-08 | 2005-08-31 | 김동철 | The Emulsification of Aralia elata Seemnn and Pueraria thunbergina Extracts |
| EP1502595A4 (en) * | 2002-04-23 | 2005-09-28 | Wang Maoxiang | An injection made from ixeris sonchifolia for treatment of cardio-cerebral vascular disease and fundus diseases and method of producing thereof |
| JP2007031412A (en) * | 2005-07-29 | 2007-02-08 | National Cancer Center-Japan | Antitumor agent |
| CN104664366A (en) * | 2014-12-18 | 2015-06-03 | 云南龙润茶业集团有限公司 | Moringa oleifera and starch composition capable of regulating insulin secretion |
| KR20200113586A (en) * | 2019-03-26 | 2020-10-07 | 안동대학교 산학협력단 | Pharmaceutical composition comprising the extract of rodgersia podophylla as an effective component for prevention or treatment of diabetes and health functional food comprising the same |
-
1993
- 1993-02-04 JP JP5017229A patent/JPH07100016B2/en not_active Expired - Lifetime
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6638540B2 (en) | 1997-05-06 | 2003-10-28 | Universitat Bern | Plant extracts for the treatment of increased bone resorption |
| CN1065716C (en) * | 1998-05-12 | 2001-05-16 | 熊军功 | Wheaten food produced with lumaychs hybrid garden sorrel |
| WO1999057984A1 (en) * | 1998-05-12 | 1999-11-18 | Jungong Xiong | Use of hybrid rumex acetosa l. in production of wheat flour-containing product |
| KR100297812B1 (en) * | 1998-07-27 | 2001-11-05 | 한수길 | Foods containing stone extracts with antihypertensive effect |
| WO2001001795A1 (en) * | 1999-06-30 | 2001-01-11 | Jungong Xiong | Use of rumex k-1 hybrid rumex acetosa for preparing menu |
| JP2001335494A (en) * | 2000-05-29 | 2001-12-04 | Okinawa Shokuryo Kk | Angiotensin-converting enzyme inhibitor |
| JP4711272B2 (en) * | 2000-05-29 | 2011-06-29 | 沖縄食糧株式会社 | Angiotensin converting enzyme inhibitor |
| KR100897656B1 (en) * | 2002-04-23 | 2009-05-14 | 왕 마오셩 | An Injection made from lxeris Sonchifolia Hance for Treatment of Cardio-Cerebral Vascular Diseases and Fundus Diseases and Method of Producing Thereof |
| EP1502595A4 (en) * | 2002-04-23 | 2005-09-28 | Wang Maoxiang | An injection made from ixeris sonchifolia for treatment of cardio-cerebral vascular disease and fundus diseases and method of producing thereof |
| JP2004115440A (en) * | 2002-09-26 | 2004-04-15 | Pola Chem Ind Inc | Calorigenic sympathetic nerve activator and composition containing the same |
| JP4637447B2 (en) * | 2002-09-26 | 2011-02-23 | ポーラ化成工業株式会社 | Heat-producing sympathetic nerve activator and composition containing the same |
| KR100511515B1 (en) * | 2002-10-08 | 2005-08-31 | 김동철 | The Emulsification of Aralia elata Seemnn and Pueraria thunbergina Extracts |
| JP2007031412A (en) * | 2005-07-29 | 2007-02-08 | National Cancer Center-Japan | Antitumor agent |
| CN104664366A (en) * | 2014-12-18 | 2015-06-03 | 云南龙润茶业集团有限公司 | Moringa oleifera and starch composition capable of regulating insulin secretion |
| KR20200113586A (en) * | 2019-03-26 | 2020-10-07 | 안동대학교 산학협력단 | Pharmaceutical composition comprising the extract of rodgersia podophylla as an effective component for prevention or treatment of diabetes and health functional food comprising the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH07100016B2 (en) | 1995-11-01 |
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