JPH0493660A - Automatic analyzer for fluorometry - Google Patents
Automatic analyzer for fluorometryInfo
- Publication number
- JPH0493660A JPH0493660A JP20502390A JP20502390A JPH0493660A JP H0493660 A JPH0493660 A JP H0493660A JP 20502390 A JP20502390 A JP 20502390A JP 20502390 A JP20502390 A JP 20502390A JP H0493660 A JPH0493660 A JP H0493660A
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- reagent
- sample
- disk
- vessel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005558 fluorometry Methods 0.000 title abstract 2
- 238000006243 chemical reaction Methods 0.000 claims abstract description 34
- 230000005291 magnetic effect Effects 0.000 claims abstract description 13
- 230000005294 ferromagnetic effect Effects 0.000 claims abstract description 12
- 238000005259 measurement Methods 0.000 claims abstract description 9
- 238000001514 detection method Methods 0.000 claims description 4
- 238000005375 photometry Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 230000001678 irradiating effect Effects 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 13
- 229910001507 metal halide Inorganic materials 0.000 abstract description 3
- 150000005309 metal halides Chemical class 0.000 abstract description 3
- 238000003756 stirring Methods 0.000 abstract description 3
- 239000000470 constituent Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 229910000831 Steel Inorganic materials 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000010959 steel Substances 0.000 description 4
- 230000005347 demagnetization Effects 0.000 description 3
- 239000003302 ferromagnetic material Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 238000009614 chemical analysis method Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000005415 magnetization Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は蛍光測定用自動分析装置に係わり、特に液体試
料の蛍光特性を次々と測定し得る蛍光測定用自動分析装
置に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to an automatic analyzer for fluorescence measurements, and more particularly to an automatic analyzer for fluorescence measurements that can successively measure the fluorescence characteristics of liquid samples.
従来の放電管を用いた蛍光測定用自動分析装置は、臨床
病理、臨時増刊、特集第５３号（１９８３年２月）第１
１４頁及び第１１５頁に記載されている。An automatic analyzer for fluorescence measurement using a conventional discharge tube is described in Clinical Pathology, Special Issue, Special Issue No. 53 (February 1983), No. 1.
It is described on pages 14 and 115.
上記装置は反応テーブル円周上に複数のキュベツトが配
列されており、蛍光測定に当たっては、キュベツト中の
反応生成物の蛍光強度をフォトマルチプライヤを検出器
として直接測光により読み取っていた。In the above-mentioned apparatus, a plurality of cuvettes are arranged around the circumference of a reaction table, and in fluorescence measurement, the fluorescence intensity of the reaction product in the cuvette is directly read by photometry using a photomultiplier as a detector.
上記従来技術に準じて分析装置を構成すると、光源部ま
たは検出部に放電管を有し、駆動部分のうち強磁性体か
ら成る部分にマグネット等、磁場を発する物体を接近さ
せると、磁化され、上記放電管に磁気的に外乱として作
用してサンプル濃度の測定値を不正確にするという問題
が生じた。When an analyzer is configured according to the above-mentioned conventional technology, the light source section or the detection section has a discharge tube, and when an object that emits a magnetic field, such as a magnet, approaches the ferromagnetic part of the driving part, it becomes magnetized. A problem has arisen in that magnetic disturbance acts on the discharge tube, making the measured value of sample concentration inaccurate.
本発明の目的は、強磁性体駆動部分を消磁し、正確な測
定ができるようにすることにある。An object of the present invention is to demagnetize the ferromagnetic drive part and enable accurate measurements.
上記目的を達成するために、鉄心と鉄心に巻かれたコイ
ルよりなる電磁石の両磁極が、上記強磁性体駆動部をは
さむように上記電磁石を設置し、上記コイルに、減衰交
番電流を流すことのできる交流発生装置を接続し、さら
に、上記減衰交番電流を上記コイルに流しながら、上記
強磁性体駆動部を動作できるようにしたものである。In order to achieve the above object, the electromagnet is installed such that both magnetic poles of an electromagnet consisting of an iron core and a coil wound around the iron core sandwich the ferromagnetic drive section, and a damped alternating current is passed through the coil. The ferromagnetic drive unit can be operated by connecting an alternating current generating device capable of generating a current to the coil and flowing the attenuated alternating current to the coil.
上記電磁石の両磁極が上記強磁性体駆動部をはさむよう
に設置されているため、上記電磁石の両磁極間に発生す
る磁力線が効率良く上記強磁性体駆動部を貫き、さらに
上記減衰交番磁場中で上記強磁性体駆動部を動作させる
ことにより、上記強磁性体駆動部全体を一様に消磁する
ことができる。Since both magnetic poles of the electromagnet are installed to sandwich the ferromagnetic drive section, the lines of magnetic force generated between the two magnetic poles of the electromagnet efficiently penetrate the ferromagnetic drive section, and furthermore, the magnetic field lines generated between the two magnetic poles of the electromagnet efficiently penetrate the ferromagnetic drive section. By operating the ferromagnetic material drive section, the entire ferromagnetic material drive section can be uniformly demagnetized.
このようにして、上記強磁性体駆動部全体が一様に消磁
されるため、光源用放電管ランプ及び検出用フォトマル
チプライヤへの、駆動部動作による磁気的外乱がなくな
り、正確な測定ができるようになる。In this way, the entire ferromagnetic drive section is uniformly demagnetized, so there is no magnetic disturbance to the light source discharge tube lamp and detection photomultiplier due to drive section operation, allowing accurate measurements. It becomes like this.
〔実施例〕 本発明の一実施例を第１図〜第４図により説明する。〔Example〕 An embodiment of the present invention will be described with reference to FIGS. 1 to 4.
回転型円盤状の反応ディスク１の円周上に複数個の反応
容器２を配列し、反応ディスク１を駆動回路３．パルス
モータ４．鋼鉄製駆動ギヤ５よりなる反応ディスク駆動
機構により回転する。A plurality of reaction vessels 2 are arranged on the circumference of a rotating disk-shaped reaction disk 1, and the reaction disk 1 is connected to a drive circuit 3. Pulse motor 4. It is rotated by a reaction disk drive mechanism consisting of a steel drive gear 5.
試薬容器６及びサンプルカップ７は、試薬試料ディスク
８の回転移動により化学的分析法により決められたタイ
ミングで吸引採取位置９へ運搬される。その後試薬及び
試料は、ピペッティング機構１０により吸引採取され、
反応ディスク１上の分注位置１１において所定の反応容
器２に、試薬試料ディスク８上の試薬容器６及びサンプ
ルカップ７より分注される。The reagent container 6 and the sample cup 7 are transported to the suction collection position 9 by the rotational movement of the reagent sample disk 8 at a timing determined by the chemical analysis method. Thereafter, the reagent and sample are aspirated by the pipetting mechanism 10,
At the dispensing position 11 on the reaction disk 1, the reagent is dispensed into a predetermined reaction container 2 from the reagent container 6 and sample cup 7 on the reagent sample disk 8.
反応容器２に分注された試薬または試料は上記反応ディ
スク回転機構により回転方向に回転移動し、撹拌機構１
５により撹拌され、反応が促進される。反応容器２中で
は反応が進行し、反応容器２が測光位置１６に到達する
と光源放電管であるメタルハライドランプ１２及び蛍光
検出器であるフォトマルチプライヤ１３を構成要素とし
て含む蛍光光度計１４により蛍光測定が行なわれ、ＡＤ
変換回路１７により、反応液の濃度に応じたデジタル信
号に変換され、データ処理回路１８において濃度データ
に変換された後、プリンタ１９に印字出力される。The reagent or sample dispensed into the reaction container 2 is rotated in the rotational direction by the reaction disk rotation mechanism, and the stirring mechanism 1
5 to accelerate the reaction. The reaction progresses in the reaction vessel 2, and when the reaction vessel 2 reaches the photometry position 16, fluorescence is measured using a fluorometer 14 that includes a metal halide lamp 12, which is a light source discharge tube, and a photomultiplier 13, which is a fluorescence detector. is carried out, AD
The conversion circuit 17 converts the signal into a digital signal corresponding to the concentration of the reaction liquid, and the data processing circuit 18 converts the signal into concentration data, which is then printed out to the printer 19 .
さらに本実施例では、磨耗の少ない鋼鉄製駆動ギア５を
使用している。鋼鉄は強磁性体なので装置内外の磁場に
よる磁化を消磁するため、スタートのたびに、駆動ギア
５を消磁する消磁装置を有する。Furthermore, in this embodiment, a steel drive gear 5 with less wear is used. Since steel is a ferromagnetic material, in order to demagnetize magnetization due to magnetic fields inside and outside the device, a demagnetizing device is provided to demagnetize the drive gear 5 every time the device is started.
消磁装置は鉄心２０と鉄心２ｏに巻いたコイル２１より
なる電磁石で、その両端が、鋼鉄製駆動ギア５の頁面に
位置するように構成されている。The degaussing device is an electromagnet consisting of an iron core 20 and a coil 21 wound around the iron core 2o, and is configured such that both ends thereof are located on the surface of the steel drive gear 5.
第３図及び第４図により、具体的な消磁電−流形状と駆
動ギア５位置を示す。3 and 4 show the specific shape of the demagnetizing current and the position of the drive gear 5.
時刻０秒において反応ディスク１がホーム位置から定速
回転を始め、３０秒後に１回転して再びホーム位置に至
る。一方、消磁電流は、５０Ｈｚのサイン波電流であり
、消磁電流の振幅は、時刻０で、最大値になっており、
直線的に減少し、時刻３０秒で反応ディスク１がホーム
位置に再び到達したときＯになる。この操作は反応ディ
スク１の１回転分即ち強磁性体駆動ギア５の１回転分て
も十分であるが、連続して２回以上行なえば、消磁効果
は向上する。第３図及び第４図は２回転の消磁動作を行
なった場合を示した。At time 0 seconds, the reaction disk 1 starts rotating at a constant speed from the home position, and after 30 seconds, it rotates once and returns to the home position. On the other hand, the degaussing current is a 50Hz sine wave current, and the amplitude of the degaussing current is the maximum value at time 0.
It decreases linearly and reaches O when the reaction disk 1 reaches the home position again at time 30 seconds. Although it is sufficient to perform this operation for one rotation of the reaction disk 1, that is, one rotation of the ferromagnetic drive gear 5, the demagnetization effect is improved if it is performed two or more times in succession. FIGS. 3 and 4 show the case where two rotations of demagnetization are performed.
また、第５図及び第６図のように１ピッチ周期で消磁動
作を行なうこともできる。ホーム位置にあった反応ディ
スク１は時刻０．８ 秒で１ピッチ回転動作を開始する
。一方、消磁電流の振幅は、時刻０で最大値となってお
り、直線的に減少し、時刻０．８ 秒でＯになる。時刻
０．８秒に１ピッチ回転動作を開始した反応ディスク１
は、時刻１秒には１ピッチ回転動作を終了している。こ
のようにして、計１２０回即ち１２０秒間同様の動作を
繰り返し、良好な消磁効果を得た。Further, as shown in FIGS. 5 and 6, the demagnetizing operation can also be performed in one pitch period. The reaction disk 1 at the home position starts rotating by one pitch at a time of 0.8 seconds. On the other hand, the amplitude of the degaussing current reaches its maximum value at time 0, decreases linearly, and reaches 0 at time 0.8 seconds. Reaction disk 1 started rotating by 1 pitch at time 0.8 seconds
has completed the one-pitch rotation operation at time 1 second. In this way, the same operation was repeated a total of 120 times, ie, for 120 seconds, and a good demagnetization effect was obtained.
本発明によれば駆動部の消磁を行なうので、光源用放電
管ランプ及び検出用フォトマルチプライヤに駆動部動作
による変動磁場が無くなり、正確な測定ができるように
なる。According to the present invention, since the driving section is demagnetized, there is no fluctuating magnetic field in the light source discharge tube lamp and the detection photomultiplier due to the operation of the driving section, making it possible to perform accurate measurements.
第１図は本発明の一実施例の駆動部まわりの図、第２図
は分析部を上から見た図、第３図は駆動ギアの位置変化
を示す図、第４図は第３図の駆動ギア位置に対応する消
磁電流を示す図、第５図は駆動ギアの位置変化の第２の
例を示す図、第６図は第５図の駆動ギア位置に対応する
消磁電流を示す図である。
１・・・反応ディスク、２・・反応容器、３・・・駆動
回路、４・・パルスモータ、Ｓ・・・駆動ギア、６・・
試薬容器、７・・・サンプルカップ、８・・・試薬試料
ディスク、９吸引採取位置、１０ ・ピペッティング機
構、１１・・・分注位置、１２・・メタルハライドラン
プ、１３ ・フォトマルチプライヤ、１４・・・蛍光光
度計、１５・撹拌機構、１６・・・測光位置、１７・・
ＡＤ変換回路、１８ ・データ処理回路、１９・・・プ
リンタ、２０・・鉄心、２１・・・コイル。
第２ 図Fig. 1 is a diagram of the drive unit in an embodiment of the present invention, Fig. 2 is a view of the analysis unit seen from above, Fig. 3 is a diagram showing changes in the position of the drive gear, and Fig. 4 is a diagram of the drive unit. FIG. 5 is a diagram showing a second example of a change in the position of the drive gear. FIG. 6 is a diagram showing the demagnetizing current corresponding to the drive gear position in FIG. 5. It is. DESCRIPTION OF SYMBOLS 1... Reaction disk, 2... Reaction container, 3... Drive circuit, 4... Pulse motor, S... Drive gear, 6...
Reagent container, 7... Sample cup, 8... Reagent sample disk, 9 Suction sampling position, 10 - Pipetting mechanism, 11... Dispensing position, 12... Metal halide lamp, 13 - Photo multiplier, 14 ...Fluorometer, 15. Stirring mechanism, 16.Photometering position, 17.
AD conversion circuit, 18 - data processing circuit, 19... printer, 20... iron core, 21... coil. Figure 2
Claims (1)
と、上記反応ディスクを駆動させるための強磁性体製部
品を含む駆動部と、上記反応容器に光を照射して上記反
応容器内の反応液を測光するための光源用ランプおよび
検出用フォトマルチプライヤを含む光度計を有する自動
分析装置において、上記強磁性体製部品を含む駆動部を
消磁するための交番磁場を発生させる消磁装置を設けた
ことを特徴とする蛍光測定用自動分析装置。1. A reaction disk in which a plurality of reaction vessels containing a sample are arranged, a drive unit including ferromagnetic parts for driving the reaction disk, and a reaction in the reaction vessel by irradiating the reaction vessel with light. In an automatic analyzer having a photometer including a light source lamp for photometry of a liquid and a photomultiplier for detection, a degaussing device is provided to generate an alternating magnetic field to demagnetize a drive unit including the above-mentioned ferromagnetic parts. An automatic analyzer for fluorescence measurement characterized by:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20502390A JPH0493660A (en) | 1990-08-03 | 1990-08-03 | Automatic analyzer for fluorometry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20502390A JPH0493660A (en) | 1990-08-03 | 1990-08-03 | Automatic analyzer for fluorometry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0493660A true JPH0493660A (en) | 1992-03-26 |
Family
ID=16500166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20502390A Pending JPH0493660A (en) | 1990-08-03 | 1990-08-03 | Automatic analyzer for fluorometry |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0493660A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109856098A (en) * | 2019-01-31 | 2019-06-07 | 陈大为 | Enhanced quantitative fluorescence analysis instrument |
-
1990
- 1990-08-03 JP JP20502390A patent/JPH0493660A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109856098A (en) * | 2019-01-31 | 2019-06-07 | 陈大为 | Enhanced quantitative fluorescence analysis instrument |
| CN109856098B (en) * | 2019-01-31 | 2021-08-20 | 陈大为 | Enhanced fluorescent quantitative analyzer |
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