JPH0470275B2 - - Google Patents
Info
- Publication number
- JPH0470275B2 JPH0470275B2 JP26244589A JP26244589A JPH0470275B2 JP H0470275 B2 JPH0470275 B2 JP H0470275B2 JP 26244589 A JP26244589 A JP 26244589A JP 26244589 A JP26244589 A JP 26244589A JP H0470275 B2 JPH0470275 B2 JP H0470275B2
- Authority
- JP
- Japan
- Prior art keywords
- fertilizer
- present
- bacteria
- cells
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003337 fertilizer Substances 0.000 claims description 28
- 241000894006 Bacteria Species 0.000 claims description 24
- 239000005416 organic matter Substances 0.000 claims description 12
- 150000003839 salts Chemical class 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 description 13
- 238000000855 fermentation Methods 0.000 description 12
- 230000004151 fermentation Effects 0.000 description 12
- 238000000034 method Methods 0.000 description 10
- 239000004484 Briquette Substances 0.000 description 9
- 238000005469 granulation Methods 0.000 description 8
- 230000003179 granulation Effects 0.000 description 8
- 239000002689 soil Substances 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 239000002994 raw material Substances 0.000 description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- 239000002374 bone meal Substances 0.000 description 4
- 229940036811 bone meal Drugs 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000011230 binding agent Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 235000013379 molasses Nutrition 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 235000019733 Fish meal Nutrition 0.000 description 2
- 235000019484 Rapeseed oil Nutrition 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 235000012343 cottonseed oil Nutrition 0.000 description 2
- 239000002385 cottonseed oil Substances 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 244000307700 Fragaria vesca Species 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 241000297434 Stenotarsus subtilis Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000187391 Streptomyces hygroscopicus Species 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 235000013527 bean curd Nutrition 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000002921 fermentation waste Substances 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000010440 gypsum Substances 0.000 description 1
- 229910052602 gypsum Inorganic materials 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004898 kneading Methods 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 238000005987 sulfurization reaction Methods 0.000 description 1
- -1 superphosphate Chemical class 0.000 description 1
- 239000002426 superphosphate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Landscapes
- Fertilizers (AREA)
Description
（産業上の利用分野）
本発明は生菌体を含有した粒状肥料に関し、更
に詳しくは、生菌数107cells／ｇ以上の発酵生産
物を用い、ブリケツト造粒機により直径３mm以上
に成形した生菌体含有粒状肥料に関する。
（従来の技術）
近年、地力低下、作物の高品質化、自然農法の
普及などにより、有機質肥料に対する需要は年々
増加の一途をたどつている。
このような有機質肥料も放線菌等の有用菌の存
在下で始めて植物に対し有効に作用するため、生
菌体添加の研究が行われてきた。
生菌体の多く着生した有機質肥料としては、古
くからきゆう堆肥が利用されているが、土壌改良
的価値はあるものの肥料的価値は殆どなく、この
ため肥料的価値の高い油粕、魚粕、骨粉等に積極
的に有用菌体を添加することも試みられたが、着
生率が低く、また粉状であるため取扱いが不便で
あり、現在殆ど利用されていない。
（発明が解決しようとする課題）
本発明者らはかかる現状に鑑み、高濃度に生菌
体を含有し、且つ無機肥料塩を含有した粒状肥料
について研究した結果、本発明を完成したもので
ある。
（課題を解決するための手段）
即ち、本発明は有機物を発酵させることにより
生菌数を107cells／ｇ以上とした発酵生産物20重
量％以上と、無機質肥料塩とを混合して直径３mm
以上にブリケツト成形した生菌体含有粒状肥料に
関する。
（作用）
本発明に於いて有機物を発酵せしめ、発酵生産
物そのものを原料として試用する理由は、有用菌
体の製品中及び土壌中に於ける着生率を高めるこ
とにある。
また、発酵物中の菌体数を107cells／ｇ以上と
する理由は、これ以下では本発明者らの研究の結
果、有機物に対して有効に作用しないことによ
る。本発明に使用する有機物としては、例えば、
魚粕、魚廃物、各種油粕、骨粉、肉粕、皮革粉、
豆腐粕、食品工業、発酵工業、ゼラチン工業等の
活性スラツジ、絹紡蚕蛹くず等を利用することが
できる。
有機物の発酵菌体としては、Streptomyces
hygroscopicus、Aspergillus oryzae、
Aspergillus niger、Saccharomyces cerevisiae、
Bacillus subtilis等を使用し、水分35〜50％、
Ｃ／Ｎ比５〜15、温度30〜60℃で、大略３〜10日
間程度で発酵すれば、有機物種、発酵条件にもよ
るが、生菌数は107cells／ｇ以上に達する。この
発酵生産物を60℃以下の温度で水分５〜15％程度
になるまで乾燥し、乾物換算でこの発酵生産物20
重量％以上とりん安、硫安、塩加、塩マグ、硝酸
カルシウム、過りん酸石灰等の無機肥料塩、必要
に応じ廃糖蜜、発酵廃液、リグニン等のバインダ
ー、石こう、ベントナイト、ゼオライト等の成分
調整材、有機物質をよく混合し、直径３mm以上に
ブリケツト成形する。
直径３mm以下のブリケツト成形品にあつては、
水分等の影響と推定されるが、製品保管、輸送中
に生菌体が死滅し望ましくない。
また、粒状肥料全体に占める発酵生産物量が20
重量％を下廻ると、菌体着生率は急激に低下す
る。肥料の造粒方法には、パン造粒、ブランジヤ
ー造粒、ドラム造粒、ペレツト造粒等種々の方法
があり、肥料種、生産量によりそれぞれ使い分け
られている。
しかしながら、例えば前三者では本発明のよう
に有機物を多用する場合、粒状品を製造すること
ができないか、見掛け上粒状品を製造することが
できても、輸送中、堆積中に粒は崩壊し、造粒条
件によつては殆ど菌は死滅する。
また、ペレツト造粒について云えば、混練時の
発熱により、殆ど菌は死滅する。
しかしながら、本発明の条件、即ち、直径３mm
以上にブリケツト成形する場合に於いては、菌は
全く死滅せず、多量の有機物を使用するにも係わ
らず、堅牢な粒状品を製造することができる。
ブリケツト造粒法は、回転軸に取り付けられた
２個のロールが互いに食い込み同速度で回転して
おり、ロールの外周に成形物の母型となるポケツ
トが刻まれている。
これらのロール間に先の混合物を供給し、連続
的に成形する方法であり、本発明にあつては、ポ
ケツトの直径が３mm以上である必要がある。
原料供給方法としては、スクリユーフイーダー
で供給する方法も考えられるが、菌体が死滅する
ので自然落下法による供給が好ましい。
菌体の生存だけで云えば、原料として全量有機
質を使用することが、粒状品の空隙率、水分維持
の点から最善であるが、施肥の省力化、即ち元
肥、追肥としての使用及び肥料という観点よりす
れば、無機−有機複合体が最も望ましい。
しかしながら、無機肥料中では生菌体は容易に
死滅するため、本発明にあつては原料に発酵生産
物を用い、直径３mm以上のブリケツト成形品とす
ることにより、造粒時及び保管時の生菌体の死滅
を防止し、施用時の着生効率を向上せしめてい
る。
従つて、本発明は生菌体を含んでいるにも拘ら
ず、取扱い容易な粒状品であつて、しかも無機−
有機の両者を含み、元肥、追肥として使用するこ
とはでき、生菌体を含有していることから土壌中
で有機物が作物及び土壌に対して極めて有効に作
用するため、土壌改善は勿論のこと、高品質の作
物を育成することができる。
（実施例）
以下に本発明の実施例を掲げて更に説明を行う
が、本発明はこれらに限定されるものではない。
また、％は特に断らない限り全て重量％を示す。
実施例 １
ナタネ油粕150Kg、魚かす150Kg、蒸製骨粉75
Kg、綿実油かす50Kgを充分混合し、これに発酵菌
体として土壌より分離培養した菌体
（Streptomyces hygroscopicus、Bacillus
subtilisを同定）の0.5Kgを接種し、水300Kgを加
え水分調整を行つた。この時の水分は43％であつ
た。
これを約１ｍの高さに山積みし、布で覆い保温
を行いながら堆積発酵を開始させた。
混合より約20時間経過後より発熱があり、40時
間経過後には50〜60℃まで上昇したので、空気の
供給と高温発酵の防止のために堆積物の切り返し
を行い、その後一日一日の切り返し作業を続けな
がら更に５日間発酵を行つた。
発酵終了後、堆積物を５〜10cmの厚さに薄く広
げ、風乾を行い水分を12％に調整し、これを奈良
式粉砕機を用いて粉砕した。
この粉砕物を50％、硫安17％、過石23％、硫加
10％となる割合で配合し、これに廃糖蜜の水溶液
をバインダーとして加えブリケツト成形した。
尚、ブリケツター・マシンは新東工業(株)製
BSS501型を使用し、ポケツト寸法は4.5mmで成形
を行つた。
この成形物をドラムドライヤーにより60℃で乾
燥し、更に篩分けを行い直径4.8mmの本発明の肥
料を得た。
比較例 １
実施例１の発酵乾燥物を15％、硫安31％、過石
35％、硫加19％となる割合で配合し、実施例１と
同様にして直径4.8mmのブリケツト成形肥料を得
た。
比較例 ２
実施例１のブリケツター・マシンのポケツト寸
法を2.0mmに変え、その他の条件は実施例１と同
様にして直径2.2mmのブリケツト成形肥料を得た。
比較例 ３
ナタネ油粕50Kg、魚粕50Kg、蒸製骨粉25Kg、綿
実油粕15Kgに、実施例１で使用した土壌より分離
培養した菌体の15Kgを加え、生菌数を107cells／
ｇ以上としたものを発酵処理を行わずに粉砕し
た。
この粉砕物を50％、硫安17％、過石23％、硫加
10％となる割合で配合し、これに廃糖蜜の水溶液
をバインダーとして加え、ブリケツター・マシン
を用いポケツト寸法を4.5mmでブリケツト成形を
行つた。
この成形物を実施例１と同様に乾燥、篩分けを
行い、直径4.8mmのブリケツト肥料を得た。
実施例１及び比較例１〜３の肥料のブリケツト
成形前（ロール投入直前）、及び成形１ケ月経過
後の生菌数の測定を行つた。
尚、生菌数の測定は、普通寒天培地（肉エキス
5.0ｇ、ペプトン10.0ｇ、塩化ナトリウム5.0ｇ、
寒天末15.0ｇ、PH7.0）を使用し、土壌微生物実
験法（(株)養賢堂）２−２−１希釈平板法で測定し
た。
尚、培養温度は30℃、培養日数は３日間で行
い、培養後に出現したコロニーの全てを生菌数と
した。
生菌数の測定結果を第１表に示した。
(Industrial Application Field) The present invention relates to a granular fertilizer containing viable bacteria, and more specifically, using a fermentation product with a viable bacterial count of 10 7 cells/g or more, it is molded into a diameter of 3 mm or more using a briquette granulator. The present invention relates to a granular fertilizer containing viable bacterial cells. (Prior Art) In recent years, the demand for organic fertilizers has been increasing year by year due to declining soil fertility, increasing quality of crops, and the spread of natural farming methods. Since such organic fertilizers only act effectively on plants in the presence of useful bacteria such as actinomycetes, research has been conducted on the addition of live bacteria. Compost has been used for a long time as an organic fertilizer with a large number of living bacteria attached to it, but although it has soil improvement value, it has almost no fertilizer value. Attempts have been made to actively add useful microbial cells to bone meal, etc., but the settlement rate is low and the powdery nature makes handling inconvenient, so it is hardly used at present. (Problems to be Solved by the Invention) In view of the current situation, the present inventors have completed the present invention as a result of research on granular fertilizers that contain viable bacteria at a high concentration and also contain inorganic fertilizer salts. be. (Means for Solving the Problem) That is, the present invention mixes 20% by weight or more of a fermentation product with a viable cell count of 10 7 cells/g or more by fermenting an organic substance and an inorganic fertilizer salt. 3mm
The above relates to a granular fertilizer containing live bacteria formed into briquettes. (Function) In the present invention, the reason why organic matter is fermented and the fermented product itself is used as a raw material is to increase the rate of attachment of useful microorganisms in the product and in the soil. Furthermore, the reason why the number of microbial cells in the fermented product is set to 10 7 cells/g or more is because, as a result of research conducted by the present inventors, the number of bacterial cells in the fermented product is less than 10 7 cells/g, and as a result of the research conducted by the present inventors, it does not act effectively on organic matter. Examples of organic substances used in the present invention include:
Fish meal, fish waste, various oil cakes, bone meal, meat meal, leather powder,
Tofu lees, activated sludge from the food industry, fermentation industry, gelatin industry, etc., silk spinning silkworm pupa waste, etc. can be used. Streptomyces is a fungus that ferments organic matter.
hygroscopicus, Aspergillus oryzae,
Aspergillus niger, Saccharomyces cerevisiae,
Using Bacillus subtilis etc., moisture 35-50%,
If fermentation is carried out at a C/N ratio of 5 to 15 and a temperature of 30 to 60° C. for about 3 to 10 days, the number of viable bacteria can reach 10 7 cells/g or more, depending on the type of organic matter and fermentation conditions. This fermented product is dried at a temperature below 60℃ until the moisture content is about 5 to 15%, and the fermented product is
Ingredients such as ammonium, ammonium sulfate, salt, salt mag, calcium nitrate, inorganic fertilizer salts such as superphosphate, blackstrap molasses, fermentation waste liquid, binder such as lignin as necessary, gypsum, bentonite, zeolite, etc. Mix the conditioning material and organic substance well and form into briquettes with a diameter of 3 mm or more. For briquette molded products with a diameter of 3 mm or less,
It is presumed that this is due to the influence of moisture, etc., but viable bacteria die during product storage and transportation, which is undesirable. In addition, the amount of fermented products in the total granular fertilizer is 20%.
When the weight percentage decreases, the bacterial cell settlement rate decreases rapidly. There are various methods for granulating fertilizers, such as pan granulation, branzier granulation, drum granulation, and pellet granulation, and each method is used depending on the type of fertilizer and production amount. However, for example, in the former three cases, when organic substances are used extensively as in the present invention, it is not possible to produce granular products, or even if granular products can be produced apparently, the granules disintegrate during transportation and deposition. However, depending on the granulation conditions, most of the bacteria will be killed. Regarding pellet granulation, most of the bacteria are killed by the heat generated during kneading. However, the conditions of the present invention, i.e., 3 mm in diameter.
In the case of briquette molding as described above, bacteria are not killed at all, and robust granular products can be produced despite the use of a large amount of organic matter. In the briquette granulation method, two rolls attached to a rotating shaft bite into each other and rotate at the same speed, and pockets are carved on the outer periphery of the rolls to serve as a matrix for the molded product. This is a method in which the mixture is fed between these rolls and continuously molded. In the present invention, the diameter of the pocket must be 3 mm or more. As a raw material supply method, a method of supplying with a screw feeder is also considered, but since the bacterial cells are killed, it is preferable to supply the raw material with a gravity drop method. In terms of bacterial survival alone, it is best to use all organic matter as a raw material in terms of the porosity of granular products and moisture retention, but it is best to use organic matter as a raw material in terms of porosity and moisture retention of granular products. From this point of view, inorganic-organic composites are most desirable. However, since living bacteria easily die in inorganic fertilizers, in the present invention, fermentation products are used as raw materials, and by forming briquettes with a diameter of 3 mm or more, it is possible to prevent the production of bacteria during granulation and storage. It prevents the death of bacterial cells and improves the settlement efficiency during application. Therefore, the present invention is a granular product that is easy to handle even though it contains live bacterial cells, and is inorganic.
It contains both organic matter and can be used as a base fertilizer and top dressing, and since it contains living bacteria, the organic matter in the soil has an extremely effective effect on crops and soil, so it is of course useful for soil improvement. , it is possible to grow high-quality crops. (Example) The present invention will be further explained below with reference to Examples, but the present invention is not limited thereto.
Moreover, all percentages indicate weight % unless otherwise specified. Example 1 Rapeseed oil cake 150Kg, fish cake 150Kg, steamed bone meal 75
Kg and 50 Kg of cottonseed oil cake were thoroughly mixed, and bacteria (Streptomyces hygroscopicus, Bacillus
0.5 kg of S. subtilis was inoculated, and 300 kg of water was added to adjust the moisture content. The moisture content at this time was 43%. This was piled up to a height of about 1 m, and piled fermentation was started while keeping it warm by covering it with cloth. Approximately 20 hours after mixing, heat generation started, and after 40 hours, the temperature rose to 50-60℃, so we removed the sediment to supply air and prevent high-temperature fermentation. Fermentation continued for another 5 days while continuing to cut back. After completion of fermentation, the deposit was spread thinly to a thickness of 5 to 10 cm, air-dried, and the moisture content was adjusted to 12%, which was then pulverized using a Nara-type pulverizer. 50% of this crushed material, 17% of ammonium sulfate, 23% of perishite, sulfurized
The mixture was blended at a ratio of 10%, an aqueous solution of blackstrap molasses was added as a binder, and briquettes were formed. The briquette machine is manufactured by Shinto Kogyo Co., Ltd.
Molding was carried out using the BSS501 model with a pocket size of 4.5 mm. This molded product was dried at 60° C. using a drum dryer and further sieved to obtain the fertilizer of the present invention having a diameter of 4.8 mm. Comparative Example 1 15% of the fermented dried product of Example 1, 31% of ammonium sulfate, and
A briquette molded fertilizer with a diameter of 4.8 mm was obtained in the same manner as in Example 1 by blending at a ratio of 35% and 19% sulfurization. Comparative Example 2 Fertilizer was formed into briquettes with a diameter of 2.2 mm using the same conditions as in Example 1 except that the pocket size of the briquetting machine was changed to 2.0 mm. Comparative Example 3 15 kg of bacterial cells isolated and cultured from the soil used in Example 1 was added to 50 kg of rapeseed oil meal, 50 kg of fish meal, 25 kg of steamed bone meal, and 15 kg of cottonseed oil meal, and the number of viable bacteria was reduced to 10 7 cells/
gram or more was pulverized without fermentation treatment. 50% of this crushed material, 17% of ammonium sulfate, 23% of perishite, sulfurized
The mixture was mixed at a ratio of 10%, an aqueous solution of blackstrap molasses was added as a binder, and briquettes were formed using a briquette machine with a pocket size of 4.5 mm. This molded product was dried and sieved in the same manner as in Example 1 to obtain briquette fertilizer with a diameter of 4.8 mm. The number of viable bacteria of the fertilizers of Example 1 and Comparative Examples 1 to 3 was measured before briquetting (immediately before being rolled) and after 1 month of briquetting. In addition, to measure the number of viable bacteria, use ordinary agar medium (meat extract).
5.0g, peptone 10.0g, sodium chloride 5.0g,
Using 15.0 g of agar powder, pH 7.0), the measurement was performed using the soil microorganism experiment method (Yokendo Co., Ltd.) 2-2-1 dilution plate method. The culture temperature was 30°C and the culture period was 3 days, and all colonies that appeared after culture were counted as viable bacteria. Table 1 shows the measurement results for the number of viable bacteria.
【表】
第１表から明らかなように、有機物を発酵させ
ることにより生菌数を107cells／ｇとした本発明
の肥料は、１ケ月の経過後に於いても殆ど生菌数
が変化しないにも係わらず、有機物に単に菌体を
混合したもの（比較例３）は、大幅な生菌数の減
少が見られる。
また、ブリケツト成形物が直径３mmを下廻る
と、あるいは発酵生産物含量が20重量％を下廻る
と、同様に生菌数の減少が見られ、これらの因子
は本発明にとつて殊に重要であることが明らかで
ある。
実施例 ２
実施例１で得た本発明の生菌体含有粒状肥料を
用い、イチゴの萎黄病に対する病害抑止効果を調
べた。
比較対象として比較例３で得た肥料を用いて行
つた。
栽培試験の条件を以下に示す。
＜栽培条件＞
供試肥料成分：（TN：Ｔ−P2O5：Ｔ−K2Ｏ）
（％）
本発明品（実施例１）6.7−6.8−6.8
対象品（比較例３）6.2−6.4−6.1
試験地：神戸市西区ハウス栽培
作 物：イチゴ（品種 宝交早生）
定 植：９月25日
施肥量：元肥（９月20日）Ｎとして12Kg／10a
追肥（10月５日）Ｎとして10Kg／10a
発病率測定日：11月19日
50株中の発病（萎黄病）株数を測定し、その結
果を第２表に示した。[Table] As is clear from Table 1, in the fertilizer of the present invention, which has a viable bacterial count of 10 7 cells/g by fermenting organic matter, the viable bacterial count hardly changes even after one month has passed. Nevertheless, in the case where bacterial cells were simply mixed with organic matter (Comparative Example 3), a significant decrease in the number of viable bacteria was observed. Furthermore, when the diameter of the briquette molded product is less than 3 mm, or when the fermentation product content is less than 20% by weight, a similar decrease in the number of viable bacteria is observed, and these factors are particularly important for the present invention. It is clear that Example 2 Using the granular fertilizer containing live bacterial cells of the present invention obtained in Example 1, the disease suppression effect on yellow wilt of strawberries was investigated. The fertilizer obtained in Comparative Example 3 was used for comparison. The conditions for the cultivation test are shown below. <Cultivation conditions> Test fertilizer components: (TN: T-P 2 O 5 : T-K 2 O)
(%) Product of the present invention (Example 1) 6.7-6.8-6.8 Target product (Comparative Example 3) 6.2-6.4-6.1 Test site: Nishi-ku, Kobe City House-grown crop: Strawberry (variety Hoko Wase) Planting: 9 Amount of fertilizer applied on the 25th of the month: Base fertilizer (September 20th) 12Kg/10a of N. Top dressing (October 5th) 10Kg/10a of N. Date of disease attack rate measurement: November 19th. Number of plants with disease (yellowing disease) out of 50 plants. The results are shown in Table 2.
Claims (1)
cells／ｇ以上とした発酵生産物20重量％以上と、
無機質肥料塩とを混合して直径３mm以上にブリケ
ツト成形した生菌体含有粒状肥料。1 Increase the number of viable bacteria to 10 7 by fermenting organic matter
20% by weight or more of the fermented product with cells/g or more,
A granular fertilizer containing viable bacteria that is mixed with inorganic fertilizer salt and formed into briquettes with a diameter of 3 mm or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26244589A JPH03126690A (en) | 1989-10-06 | 1989-10-06 | Living bacterium-containing granular fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26244589A JPH03126690A (en) | 1989-10-06 | 1989-10-06 | Living bacterium-containing granular fertilizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03126690A JPH03126690A (en) | 1991-05-29 |
| JPH0470275B2 true JPH0470275B2 (en) | 1992-11-10 |
Family
ID=17375891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP26244589A Granted JPH03126690A (en) | 1989-10-06 | 1989-10-06 | Living bacterium-containing granular fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03126690A (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2519623B2 (en) * | 1992-01-21 | 1996-07-31 | 嗣生 鈴木 | Fertilizer manufacturing method |
| JP2750997B2 (en) * | 1993-11-01 | 1998-05-18 | 多木化学株式会社 | Granulated fermented chicken dung |
| KR20020008486A (en) * | 2000-07-20 | 2002-01-31 | 강준구 | Fertilizer for agriculture |
| CN103332982B (en) * | 2013-06-27 | 2015-04-29 | 重庆拓阳科技有限公司 | Method for preparing special composite microbial fertilizer for non-deciduous fruit trees and capable of improving soil, and prepared product |
| CN104892316A (en) * | 2015-05-29 | 2015-09-09 | 洋县益民绿色农业科技有限责任公司 | Preparation method of special biofertilizer for citrus |
| CN104829281A (en) * | 2015-05-29 | 2015-08-12 | 洋县益民绿色农业科技有限责任公司 | Preparation method of bio-fertilizer special for apples |
| CN105622180A (en) * | 2015-12-17 | 2016-06-01 | 江苏同盛环保技术有限公司 | Process of using garden waste and dewatered sludge to produce landscaping cultivation medium |
-
1989
- 1989-10-06 JP JP26244589A patent/JPH03126690A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03126690A (en) | 1991-05-29 |
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