JPH04501355A - 白血病及びリンパ腫におけるモノクローナリテイーの診断法 - Google Patents
白血病及びリンパ腫におけるモノクローナリテイーの診断法Info
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- JPH04501355A JPH04501355A JP1510945A JP51094589A JPH04501355A JP H04501355 A JPH04501355 A JP H04501355A JP 1510945 A JP1510945 A JP 1510945A JP 51094589 A JP51094589 A JP 51094589A JP H04501355 A JPH04501355 A JP H04501355A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/112—Disease subtyping, staging or classification
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
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- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (17)
- 1.組織の試料中の免疫グロブリン遺伝子及び/又はT−リセプタ遺伝子のセグ メント長の均一性又は不均一性を判定して該試料中のB−及び/又はT−リンパ 球集団のモノクローナリティーの存否を表示することを含んで成る、組織試料に おけるリンパ系白血病及び/又はリンパ腫の検出法。
- 2.(a)組織の試料中の免疫グロブリン遺伝子及び/又はT−リセプタ遺伝子 のセグメントをそれらセグメントのための特異性プライマー又は特異性プライマ ー混合物を使用するポリメラーゼの連鎖反応で拡大し、そして (b)拡大されたセグメントを大きさに基づいて分離してそれら拡大セグメント の長さの均一性又は不均一性を判定する 工程を含んで成る、組織試料におけるモノクローナリティー及び推定悪性腫瘍を 検出し、及び/又は腫瘍のB−リンパ球又はT−リンパ球の起源を判定する方法 。
- 3.該プライマーが免疫グロブリン遺伝子及び/又はT−リセプタ遺伝子のセグ メントのためのコンセンサスプライマーである、請求の範囲第2項に記載の方法 。
- 4.該組織試料が血液、節又は骨髄における腫瘍組織、ホルマリン固定され、包 埋された組織材料、アスピレートされた細胞材料及びスライドガラス上の細胞か ら選択されたものである、請求の範囲第1項又は第2項に記載の方法。
- 5.大きさに基づいて分離する該工程が電気泳動法及びクロマトグラフィーから 選択されたものである、請求の範囲第2項に記載の方法。
- 6.大きさに基づく該分離工程がアガロースゲル又はポリアクリルアミドゲルに よる電気泳動法から成る、請求の範囲第5項に記載の方法。
- 7.大きさに基づいて分離され、拡大された該遺伝子のセグメントを非同位体手 段、放射性同位体の組み込み、又は内部プローブヘのサウザン転移又はハイブリ ダイゼーションによって検出する、請求の範囲第2項に記載の方法。
- 8.非同位体手段による該検出がエチジウムブロマイド染色法を含んで成る、請 求の範囲第7項に記載の方法。
- 9.免疫グロブリン遺伝子のセグメントのための該プライマーが免疫グロブリン のV領域のフレームワーク部に対するプライマー及び該遺伝子のD.J及び/又 はC領域の一部分に対するプライマーから成る、請求の範囲第2項に記載の方法 。
- 10.免疫グロブリン遺伝子のセグメントのための該プライマーが 【配列があります】 (ただし、Nはヌクレオチド塩基のうちのいずれかを表わす) から選択されたものである、請求の範囲第9項に記載の方法。
- 11.T−リセプタ遺伝子のセグメントのための該プライマーがT−リセプタ遺 伝子の保存V領域に対するプライマー及び該遺伝子のD.J及び/又はC領域の 一部分に対するプライマーから成る、請求の範囲第2項に記載の方法。
- 12.T−リセプタ遺伝子のセグメントのための該プライマーが (a)T−リセプタのα−鎖プライマー:【配列があります】 (b)T−リセプタのβ−鎖プライマー:【配列があります】 (c)T−リセプタのγ−鎖プライマー:【配列があります】 から選択されたものである、請求の範囲第11項に記載の方法。
- 13.T−リセプタ遺伝子のセグメントのための該プライマーが V−領域 プライマー配列 【配列があります】 J−領域 プライマー配列 【配列があります】 から選択された、V−領域及びJ−領域のためのT−リセプタ遺伝子のγ−鎖プ ライマーの混合物から成る、請求の範囲第11項に記載の方法。
- 14.組織の試料中の免疫グロブリン遺伝子及び/又はT−リセプタ遺伝子のセ グメントをポリメラーゼの連鎖反応で拡大するための、免疫グロブリン遺伝子及 び/又はT−リセプタ遺伝子のセグメント用の1組の特異性プライマー又は特異 性プライマー混合物を含んで成る、請求の範囲第2項に記載の方法を実施するた めのキット。
- 15.免疫グロブリン遺伝子及び/又はT−リセプタ遺伝子のセグメントのため の1組のコンセンサスプライマーを含んで成る、請求の範囲第14項に記載のキ ット。
- 16.請求の範囲第9項または第10項に記載の、免疫グロブリン遺伝子のセグ メントのためのプライマーを含んで成る、請求の範囲第14項に記載のキット。
- 17.請求の範囲第11項若しくは第12項又は第13項に記載の、T−リセプ タ遺伝子のセグメントのためのプライマーを含んで成る、請求の範囲第14項に 記載のキット。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AUPJ105788 | 1988-10-20 | ||
AU1057 | 1999-06-18 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH04501355A true JPH04501355A (ja) | 1992-03-12 |
JP2781438B2 JP2781438B2 (ja) | 1998-07-30 |
Family
ID=3773456
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1510945A Expired - Lifetime JP2781438B2 (ja) | 1988-10-20 | 1989-10-20 | 白血病及びリンパ腫におけるモノクローナリテイーの診断法 |
Country Status (3)
Country | Link |
---|---|
US (2) | US5296351A (ja) |
JP (1) | JP2781438B2 (ja) |
WO (1) | WO1990004648A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007515154A (ja) * | 2003-05-13 | 2007-06-14 | モノクワント プロプライアタリー リミティド | 例えばt細胞受容体v/d/j遺伝子内の反復配列によるクローン細胞の同定 |
JP2007515955A (ja) * | 2003-12-05 | 2007-06-21 | アンスティテュ ナシオナル ド ラ サント エ ド ラ ルシュルシェ メディカル(アンセルム) | 個体の再構成又は標的遺伝的組換えの定量的評価の方法及びその使用 |
Families Citing this family (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9002625D0 (en) * | 1990-02-06 | 1990-04-04 | Univ Singapore | Human leukocyte antigen typing |
US7294712B2 (en) | 1990-06-04 | 2007-11-13 | Aventis Pharma S.A. | Nucleotide sequences coding for variable regions of β chains of human T lymphocyte receptors, corresponding peptide segments and the diagnostic and therapeutic uses |
FR2671356B1 (fr) * | 1991-01-09 | 1993-04-30 | Inst Nat Sante Rech Med | Procede de description des repertoires d'anticorps (ab) et des recepteurs des cellules t (tcr) du systeme immunitaire d'un individu. |
JPH06502997A (ja) | 1991-02-12 | 1994-04-07 | ルセル ユクラフ | ヒトTリンパ球レセプターのβ鎖の可変領域をコードするヌクレオチド配列対応するペプチドセグメント並びに診断及び治療への利用 |
EP0653493A4 (en) * | 1992-04-30 | 1996-03-27 | Taisho Pharmaceutical Co Ltd | METHOD FOR DETECTING GENE EXPRESSION FROM THE T-CELL ANTIGEN RECEPTOR. |
FR2693209B1 (fr) * | 1992-07-03 | 1994-09-30 | Inst Nat Sante Rech Med | Procédé de détermination de la taille en nucléotides de fragments d'ADN. |
US5668006A (en) * | 1992-07-17 | 1997-09-16 | American Cyanamid Company | Somatostatin receptors |
US6087096A (en) * | 1995-11-13 | 2000-07-11 | Dau; Peter C. | Method of intrafamily fragment analysis of the T cell receptor α and β chain CDR3 regions |
AU736742B2 (en) * | 1996-06-03 | 2001-08-02 | Governors Of The University Of Alberta, The | Methods for detection of rearranged DNA |
WO1999015696A1 (en) * | 1997-09-19 | 1999-04-01 | Medelys Laboratories Inc. | Method and kit for early diagnosis of autoimmunity and lymphoma in central nervous system |
AUPQ687600A0 (en) * | 2000-04-13 | 2000-05-11 | Flinders Technologies Pty Ltd | A method of detection |
US6596492B2 (en) | 2000-07-11 | 2003-07-22 | Colorado State University Research Foundation | PCR materials and methods useful to detect canine and feline lymphoid malignancies |
CN101074450A (zh) | 2001-09-24 | 2007-11-21 | 一兰姆达公司 | 诊断探针检测*** |
ES2354068T3 (es) * | 2002-10-11 | 2011-03-09 | Erasmus Universiteit Rotterdam | Cebadores de amplificación de ácidos nucleicos para estudios de clonalidad basada en pcr. |
US20070020670A1 (en) * | 2005-07-07 | 2007-01-25 | Hematologics, Inc. | Methods for detecting and confirming minimal disease |
US20100035764A1 (en) * | 2006-09-26 | 2010-02-11 | St. Jude Children's Research Hospital | Methods and compositions for monitoring t cell receptor diversity |
EP2062982A1 (fr) | 2007-11-26 | 2009-05-27 | ImmunID | Procédé d'étude de la diversité combinatoire V(D)J |
US8628927B2 (en) | 2008-11-07 | 2014-01-14 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
US9528160B2 (en) | 2008-11-07 | 2016-12-27 | Adaptive Biotechnolgies Corp. | Rare clonotypes and uses thereof |
US9506119B2 (en) | 2008-11-07 | 2016-11-29 | Adaptive Biotechnologies Corp. | Method of sequence determination using sequence tags |
US9365901B2 (en) | 2008-11-07 | 2016-06-14 | Adaptive Biotechnologies Corp. | Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia |
US8691510B2 (en) * | 2008-11-07 | 2014-04-08 | Sequenta, Inc. | Sequence analysis of complex amplicons |
AU2009311588B2 (en) | 2008-11-07 | 2016-07-28 | Adaptive Biotechnologies Corp. | Methods of monitoring conditions by sequence analysis |
US8748103B2 (en) | 2008-11-07 | 2014-06-10 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
US9394567B2 (en) | 2008-11-07 | 2016-07-19 | Adaptive Biotechnologies Corporation | Detection and quantification of sample contamination in immune repertoire analysis |
HUE029424T2 (en) | 2009-01-15 | 2017-02-28 | Adaptive Biotechnologies Corp | Adaptive immunity profiling and a method for producing monoclonal antibodies |
KR20120044941A (ko) | 2009-06-25 | 2012-05-08 | 프레드 헛친슨 켄서 리서치 센터 | 적응 면역의 측정방법 |
US9043160B1 (en) | 2009-11-09 | 2015-05-26 | Sequenta, Inc. | Method of determining clonotypes and clonotype profiles |
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Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
CA1305912C (en) * | 1987-06-22 | 1992-08-04 | Richard J. Albertini | Detection of lymphocyte amplification |
-
1989
- 1989-10-20 WO PCT/AU1989/000455 patent/WO1990004648A1/en unknown
- 1989-10-20 JP JP1510945A patent/JP2781438B2/ja not_active Expired - Lifetime
- 1989-10-20 US US07/684,889 patent/US5296351A/en not_active Expired - Lifetime
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2007515154A (ja) * | 2003-05-13 | 2007-06-14 | モノクワント プロプライアタリー リミティド | 例えばt細胞受容体v/d/j遺伝子内の反復配列によるクローン細胞の同定 |
JP2013116116A (ja) * | 2003-05-13 | 2013-06-13 | Monoquant Pty Ltd | T細胞受容体v/d/j遺伝子内の反復配列によるクローン細胞の同定 |
JP2016027825A (ja) * | 2003-05-13 | 2016-02-25 | モノクワント プロプライアタリー リミティド | T細胞受容体v/d/j遺伝子内の反復配列によるクローン細胞の同定 |
JP2007515955A (ja) * | 2003-12-05 | 2007-06-21 | アンスティテュ ナシオナル ド ラ サント エ ド ラ ルシュルシェ メディカル(アンセルム) | 個体の再構成又は標的遺伝的組換えの定量的評価の方法及びその使用 |
Also Published As
Publication number | Publication date |
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JP2781438B2 (ja) | 1998-07-30 |
US5296351A (en) | 1994-03-22 |
US5418134A (en) | 1995-05-23 |
WO1990004648A1 (en) | 1990-05-03 |
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