JPH0442040A - Absorbance measuring cell - Google Patents
Absorbance measuring cellInfo
- Publication number
- JPH0442040A JPH0442040A JP14912890A JP14912890A JPH0442040A JP H0442040 A JPH0442040 A JP H0442040A JP 14912890 A JP14912890 A JP 14912890A JP 14912890 A JP14912890 A JP 14912890A JP H0442040 A JPH0442040 A JP H0442040A
- Authority
- JP
- Japan
- Prior art keywords
- tube
- light
- tubes
- cell
- absorbance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002835 absorbance Methods 0.000 title claims abstract description 12
- 238000005259 measurement Methods 0.000 claims abstract description 20
- 239000004033 plastic Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 4
- 238000011481 absorbance measurement Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 7
- 239000004809 Teflon Substances 0.000 abstract description 5
- 229920006362 Teflon® Polymers 0.000 abstract description 5
- 238000004811 liquid chromatography Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 description 16
- 238000000034 method Methods 0.000 description 10
- 238000000576 coating method Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000031700 light absorption Effects 0.000 description 3
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- LQNUZADURLCDLV-UHFFFAOYSA-N nitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC=C1 LQNUZADURLCDLV-UHFFFAOYSA-N 0.000 description 2
- 238000000424 optical density measurement Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000002981 blocking agent Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】 ［産業上の利用分野］ 本発明は、吸光度測定用セルに関するものである。[Detailed description of the invention] [Industrial application field] The present invention relates to a cell for measuring absorbance.
［従来の技術］
吸光度測定用セルは、物質の吸光特性を測定するための
容器であり、このセルに測定光を透過させ、その入口側
と出口側の光量差から、吸光量を測定するものである（
ＯＤ測測定いう）。[Prior Art] An absorbance measurement cell is a container for measuring the light absorption characteristics of a substance. Measurement light is transmitted through this cell, and the amount of light absorption is measured from the difference in light intensity between the entrance and exit sides. It is (
OD measurement).
ＯＤ測測定、混合溶液の分析、分取に用いられるもので
、その吸収特性を既知のカーブと比較して決定するもの
である。即ち、セル中に導入された液の吸光度を連続的
に測定し、その物質が何か及びその量を判定するのであ
る。It is used for OD measurement, analysis of mixed solutions, and fractionation, and its absorption characteristics are determined by comparing it with known curves. That is, the absorbance of the liquid introduced into the cell is continuously measured to determine the substance and amount thereof.
このセルは、通常、測定部とチューブからなり測定部は
測定光が透過する容器であり、チューブは外部機器との
連結用配管である。This cell usually consists of a measuring section and a tube, the measuring section being a container through which measurement light passes, and the tube being a pipe for connection to external equipment.
測定部はガラス製が多く、チューブはステンレスやテフ
ロンである。これは、被測定物質と反応しないためであ
る。The measuring part is often made of glass, and the tube is made of stainless steel or Teflon. This is because it does not react with the substance to be measured.
［発明が解決しようとする課題］ この従来のセルには次のような欠点があった。[Problem to be solved by the invention] This conventional cell had the following drawbacks.
まず、吸光度の大きい物質にあっては、透過する光量が
非常に少なく、１０００分の１以下の場合もある。これ
は、物質固有の値であり、紫外線を非常によく吸収する
もの（例えば、ニトロベンゼンやアントラセン等）では
このような数値になる。First, for substances with high absorbance, the amount of light transmitted is very small, sometimes less than 1/1000. This is a value specific to the substance, and substances that absorb ultraviolet rays very well (for example, nitrobenzene and anthracene) will have such a value.
通常、この分野では吸光度はＯＤ値で表わし、入射光（
ここでは紫外線）量Ａと透過光量Ｂとの比の逆数の対数
である。Usually, in this field, absorbance is expressed as an OD value, and the incident light (
Here, it is the logarithm of the reciprocal of the ratio between the amount A of ultraviolet rays and the amount B of transmitted light.
ＯＤ＝ ｌ ｏ ｇ （Ｂ／Ａ）
このＯＤ値で３を越えるような物質では、測定部では非
常に少量の透過光を測定しなければならないため、測定
精度を高（しなければならないことは当然であるが、測
定精度をいくら高くしても、外部から紫外線や可視光が
測定部に侵入すると、それが少量であっても測定に大き
な誤差を与えることとなる。この外部光（迷光）の侵入
は、そのほとんどがチューブからであるため、ステンレ
ス製のものを使用すれば問題はないが、物質によっては
、金属が溶出する場合があり好ましくはない。OD = l o g (B/A) For substances whose OD value exceeds 3, the measuring section must measure a very small amount of transmitted light, so the measurement accuracy must be high ( Of course, no matter how high the measurement accuracy is, if ultraviolet rays or visible light from the outside enters the measuring section, even a small amount will cause a large error in the measurement.This external light (stray light) Most of the intrusion is through the tube, so if you use a stainless steel one, there will be no problem, but depending on the substance, the metal may elute, which is not preferable.
迷光が問題となるのは、迷光の量に比較して透過光量が
少ないときであり、透過光量の多い（吸光量の少ない）
物質ではあまり問題とならない。Stray light becomes a problem when the amount of transmitted light is small compared to the amount of stray light, and the amount of transmitted light is large (the amount of light absorbed is low).
This is not a problem with materials.
そこで、透過光量の少ない物質においてもできるだけ透
過光量を多くする方法を採用して、迷光の影響を軽減す
ることが考えられる。この方法は次の通りである。Therefore, it is conceivable to reduce the influence of stray light by adopting a method of increasing the amount of transmitted light as much as possible even in a material with a small amount of transmitted light. This method is as follows.
■ 吸光度は光路長１質中を紫外線が通過する距離）に
比例するため、光路長を短くする。- Absorbance is proportional to the optical path length (distance that ultraviolet rays pass through the material), so shorten the optical path length.
■ 物質の濃度を下げる
この内、■の光路長を短くする方法では、圧力損失や加
工精度の面から０．１ｍｍが限界であり、これでは前記
した高吸光度物質の測定はできず、■の濃度を下げる方
法では、クロマトのピークがブロードになり（ピークの
分離が悪くなり）、且つ液量が大きくなるため、実際に
は行なわれない。■ Decrease the concentration of the substance Among these methods, the limit of the method (■) of shortening the optical path length is 0.1 mm from the viewpoint of pressure loss and processing accuracy, and this method cannot measure the high absorbance substances mentioned above, and the method (■) shortens the optical path length. The method of lowering the concentration makes the chromatographic peaks broader (poor peak separation) and increases the amount of liquid, so it is not actually carried out.
よって、これらの透過光量を増加させる方法は現実的で
はない。Therefore, methods of increasing the amount of transmitted light are not realistic.
また、ＯＤ値が３以上の物質であっても、濃度が低い場
合には測定可能であったが、濃度による測定限界があり
、実際にはほとんど使用できないのが現状である。In addition, it has been possible to measure substances with an OD value of 3 or more if the concentration is low, but there is a measurement limit depending on the concentration, and at present it is almost impossible to use in practice.
そこで、本業界では、このような高吸光度物質の前記測
定限界をできるだけ高（すること（できるだけ高濃度で
測定できるよう）、及び正確に測定できるセルが待望さ
れていた。Therefore, in this industry, there has been a long-awaited desire to increase the measurement limit of such a highly absorbent substance as much as possible (so as to be able to measure as high a concentration as possible), and to have a cell that can accurately measure it.
［課題を解決するための手段］
以上のような現状に鑑み、本発明者は鋭意研究の結果、
本発明セルを完成させたものであり、その特徴とすると
ころは、液体の吸光度を測定するための容器であって、
測定光を透過する部分である測定部を保持する保持容器
と、該測定部と直接又は間接的に外部機器とを連結する
ためのプラスチック製チューブとからなるものにおいて
、該チューブが外部光を透過しないよう構成された点に
ある。[Means for Solving the Problems] In view of the above-mentioned current situation, the present inventor has conducted intensive research and has discovered the following.
The cell of the present invention has been completed, and its characteristics include a container for measuring the absorbance of a liquid,
A holding container that holds a measurement section that transmits measurement light, and a plastic tube that connects the measurement section directly or indirectly to an external device, where the tube transmits external light. The point is that it is configured so that it does not.
ここで、測定部とは、テフロン製の長円形の貫通孔を有
する円盤状板をガラスで両方から挟持したものや、ガラ
ス製の容器が一般的であるが、特に限定するものではな
い。Here, the measuring part is generally a disc-shaped plate having an oblong through hole made of Teflon sandwiched between glass plates, or a container made of glass, but is not particularly limited.
また、保持容器とは、上記の測定部が収容され測定光の
通過位置に測定部を固定する金属製容器であり、この容
器には外部機器との連結に用いるチューブが入口と出口
に設けられている。Furthermore, the holding container is a metal container that houses the measurement section described above and fixes the measurement section at the position where the measurement light passes, and this container has tubes at the entrance and exit for connection with external equipment. ing.
測定光とは、測定に用いる光であり、被測定物質によっ
て異なるが、通常は紫外線であるが、可視光や赤外線の
場合もある。The measurement light is light used for measurement, and although it differs depending on the substance to be measured, it is usually ultraviolet light, but may also be visible light or infrared light.
外１？’ｔｓ機器とは、液体クロマト装置、ポンプ、バ
ルブ、他のチューブ等の配管等である。Outside 1? 'ts equipment includes liquid chromatography equipment, pumps, valves, other piping such as tubes, etc.
チューブは、テフロンその他のプラスチック製であり、
紫外線及び可視光がほとんど透過しない構造となってい
る。The tube is made of Teflon or other plastic.
It has a structure that hardly transmits ultraviolet rays and visible light.
外部光とは、測定部に侵入する迷光をいい、通常は太陽
光や照明用光である。その成分から、紫外線、可視光、
赤外線等を含むものである。External light refers to stray light that enters the measuring section, and is usually sunlight or illumination light. From its components, ultraviolet rays, visible light,
This includes infrared rays, etc.
外部光を透過しない構造とは、チューブを製造する場合
に、顔料（外部光非透過物質）を混合して成型するか、
混合して重合するか等の内部着色方法でも、透明チュー
ブを製造した後に、外部に被覆材で被覆するか、外部に
遮光剤を塗布するか等の外部被覆方法のどちらでもよい
、中でも、透明チューブの外側に、遮光性の熱収縮性プ
ラスチックチューブを位置させて加熱して透明チューブ
に被覆する方法が簡単である。A structure that does not transmit external light means that when manufacturing the tube, a pigment (substance that does not transmit external light) is mixed and molded, or
Either an internal coloring method, such as mixing and polymerization, or an external coating method, such as coating the outside with a coating material or coating the outside with a light blocking agent after manufacturing the transparent tube, is acceptable. A simple method is to place a light-shielding heat-shrinkable plastic tube outside the tube and heat it to coat the transparent tube.
勿論、紫外線も可視光も厳密に１００％遮断する必要は
なく、測定に影響がでない程度でよいことは本発明の目
的から明らかである。しかし、どちらも９０％以上は遮
断することが望ましい。Of course, it is clear from the purpose of the present invention that it is not necessary to strictly block 100% of both ultraviolet rays and visible light, and it is sufficient that it does not affect the measurement. However, it is desirable to block 90% or more of both.
このように、チューブを外部光に関して遮断すると、測
定部に外部から迷光が入らず、上記した迷光による測定
誤差が生じない。In this way, when the tube is shielded from external light, stray light does not enter the measuring section from the outside, and the measurement error due to the above-mentioned stray light does not occur.
本発明セルは、分析用装置にも、分取用装置にも使用で
きる。The cell of the present invention can be used both as an analytical device and a preparative device.
［実施例コ
以下図面に示す実施例に基づいて、本発明をより詳細に
説明する。[Embodiments] The present invention will be described in more detail below based on embodiments shown in the drawings.
第１図は、本発明セル１０１例を示す斜視図であり、測
定部２を有する保持容器３の入口側と出口側にチューブ
４を設けたものである。FIG. 1 is a perspective view showing an example of a cell 101 of the present invention, in which tubes 4 are provided on the inlet and outlet sides of a holding container 3 having a measuring section 2.
このチューブ４は、テフロンで成型されたチューブ本体
に紫外線を透過しない熱収縮性プラスチックチューブを
被せ、加熱することにより該チューブ本体に被覆させた
ものである。チューブ４の先端部には液体クロマト装置
等の外部機器（図示せず）に接続するための接続具５が
設けられている。このため、セル１を外部機器に接続す
る場合露呈する部分であるチューブ４が被覆されている
ことによって、測定部２には外部からほとんど紫外線が
侵入しないこととなる。This tube 4 is made by covering a tube body made of Teflon with a heat-shrinkable plastic tube that does not transmit ultraviolet rays, and heating the tube body to cover the tube body. A connector 5 for connecting to an external device (not shown) such as a liquid chromatography device is provided at the tip of the tube 4. Therefore, since the tube 4, which is a portion exposed when the cell 1 is connected to an external device, is covered, almost no ultraviolet rays enter the measuring section 2 from the outside.
［発明の効果］
本発明セルによると、外部迷光がほとんど侵入しないた
め、ＯＤ値が高い物質でも吸光特性を測定できる。また
、非常にＯＤ値が高い物質の場合には、その測定可能な
濃度が著しく向上する。[Effects of the Invention] According to the cell of the present invention, almost no external stray light enters, so that even substances with high OD values can be measured for light absorption characteristics. Furthermore, in the case of a substance with a very high OD value, its measurable concentration is significantly improved.
さらに、本発明自体構造が簡単で、安価でありすでに完
成しているセルにも容易に実施できるという利点もある
。Furthermore, the present invention itself has the advantage that it is simple in structure, inexpensive, and can be easily implemented in already completed cells.
第１図は、本発明セルの１実施例を示す斜視図である。 １・・・セル ２・・・測定部 ３・・・保持容器 ４・・・チューブ ５・・・接続具 FIG. 1 is a perspective view showing one embodiment of the cell of the present invention. 1...Cell 2...Measuring part 3... Holding container 4...Tube 5... Connection tool
Claims (1)
光を透過する部分である測定部を保持する保持容器と、
該測定部と直接又は間接的に外部機器とを連結するため
のプラスチック製チューブとからなるものにおいて、該
チューブが外部光を透過しないよう構成されたものであ
ることを特徴とする吸光度測定用セル。 ２、該チューブは、着色された熱収縮性プラスチックで
被覆されたものである請求項１記載の吸光度測定用セル
。[Claims] 1. A holding container that holds a measurement part that is a container for measuring the absorbance of a liquid and is a part that transmits measurement light;
A cell for measuring absorbance, comprising a plastic tube for directly or indirectly connecting the measuring section to an external device, wherein the tube is configured so as not to transmit external light. . 2. The absorbance measurement cell according to claim 1, wherein the tube is coated with a colored heat-shrinkable plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14912890A JPH0442040A (en) | 1990-06-07 | 1990-06-07 | Absorbance measuring cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14912890A JPH0442040A (en) | 1990-06-07 | 1990-06-07 | Absorbance measuring cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0442040A true JPH0442040A (en) | 1992-02-12 |
Family
ID=15468345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14912890A Pending JPH0442040A (en) | 1990-06-07 | 1990-06-07 | Absorbance measuring cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0442040A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8728619B2 (en) | 2010-02-19 | 2014-05-20 | Toyo Boseki Kabushiki Kaisha | Highly functional polyethylene fiber excellent in forming processability |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5555240A (en) * | 1978-10-20 | 1980-04-23 | Toshiba Corp | Flow cell |
| JPS6215439A (en) * | 1985-07-13 | 1987-01-23 | Shimadzu Corp | Flow cell for absorptiometry |
| JPS6234288U (en) * | 1985-08-19 | 1987-02-28 |
-
1990
- 1990-06-07 JP JP14912890A patent/JPH0442040A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5555240A (en) * | 1978-10-20 | 1980-04-23 | Toshiba Corp | Flow cell |
| JPS6215439A (en) * | 1985-07-13 | 1987-01-23 | Shimadzu Corp | Flow cell for absorptiometry |
| JPS6234288U (en) * | 1985-08-19 | 1987-02-28 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8728619B2 (en) | 2010-02-19 | 2014-05-20 | Toyo Boseki Kabushiki Kaisha | Highly functional polyethylene fiber excellent in forming processability |
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