JPH0413700A - Isolation of collagen - Google Patents
Isolation of collagenInfo
- Publication number
- JPH0413700A JPH0413700A JP11801690A JP11801690A JPH0413700A JP H0413700 A JPH0413700 A JP H0413700A JP 11801690 A JP11801690 A JP 11801690A JP 11801690 A JP11801690 A JP 11801690A JP H0413700 A JPH0413700 A JP H0413700A
- Authority
- JP
- Japan
- Prior art keywords
- collagen
- fat
- distilled water
- filter
- human body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920001436 collagen Polymers 0.000 title claims abstract description 26
- 102000008186 Collagen Human genes 0.000 title claims abstract description 25
- 108010035532 Collagen Proteins 0.000 title claims abstract description 25
- 238000002955 isolation Methods 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000012153 distilled water Substances 0.000 claims abstract description 9
- 210000001789 adipocyte Anatomy 0.000 claims abstract description 6
- 239000012634 fragment Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 2
- 210000003491 skin Anatomy 0.000 description 9
- 206010020751 Hypersensitivity Diseases 0.000 description 4
- 208000032544 Cicatrix Diseases 0.000 description 3
- 238000002316 cosmetic surgery Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 231100000241 scar Toxicity 0.000 description 3
- 230000037387 scars Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 206010039580 Scar Diseases 0.000 description 1
- 206010040954 Skin wrinkling Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 102000034240 fibrous proteins Human genes 0.000 description 1
- 108091005899 fibrous proteins Proteins 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 235000015205 orange juice Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
この発明はコラーゲンの分離方法に関し、特には人体の
脂肪からコラーゲンを分離する方法に関する。DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to a method for separating collagen, and particularly to a method for separating collagen from human fat.
（従来の技術）
従来から、美容外科の分野では、例えば老人性のシワ取
りあるいは傷あと等の凹陥部の補修等の目的のために、
皮膚にコラーゲンを注入することが行なわれている。コ
ラーゲンは骨や皮膚等を形成する結合組織の主成分で繊
維状タンパク質からなるものであるから、これを注射器
で直接皮膚内に注入することによって、前記凹陥部分を
平坦化させることができる。(Prior Art) Conventionally, in the field of cosmetic surgery, for the purpose of removing senile wrinkles or repairing depressions such as scars, etc.
Collagen is injected into the skin. Collagen is a fibrous protein that is the main component of connective tissue that forms bones, skin, etc., so by injecting it directly into the skin with a syringe, the depressed area can be flattened.
従来のこの種美容外科に使用されるコラーゲンとしては
牛皮から抽出し精製したものが一般的であるが、しか・
しながら、当然人体とは異種タンパクであるため、アレ
ルギー反応を起こすおそれがある。Conventionally, the collagen used in this type of cosmetic surgery is generally extracted from cow skin and purified.
However, since the protein is foreign to the human body, there is a risk of allergic reactions.
（発明が解決しようとする課題）
そこで、本発明者は、現在美容外科で一般に多用されて
いるところの人体からの脂肪抽出に鑑み、この抽出され
た脂肪から主として本人が使用するためにコラーゲンを
取り出すことを見い出した。(Problems to be Solved by the Invention) Therefore, in view of the extraction of fat from the human body, which is currently commonly used in cosmetic surgery, the present inventors sought to extract collagen from the extracted fat mainly for use by the person himself/herself. I found out how to take it out.
本人の脂肪から分離されたコラーゲンであれば従来のよ
うな異種タンパクによるアレルギー反応は全く起きない
のである。If the collagen is separated from the person's own fat, there will be no allergic reactions caused by foreign proteins like in the past.
この発明はこのような観点にもとづき１人体の脂肪から
コラーゲンを簡単かつ効率良く分離する方法を提案する
ものである。Based on this viewpoint, the present invention proposes a method for easily and efficiently separating collagen from fat in a human body.
（課題を解決するための手段）
すなわち、この発明は、人体より抽出した脂肪細片を若
干の蒸留水とともにフィルターに通過せしめることによ
って脂肪細胞の中からコラーゲンを分離、取出すことを
特徴とするコラーゲンの分離方法に係る。(Means for Solving the Problems) That is, the present invention is characterized in that collagen is separated and extracted from fat cells by passing fat fragments extracted from the human body through a filter together with some distilled water. related to the separation method.
（実施例）
以下実施例についてこの発明方法の一実施例を説明する
。(Example) An example of the method of this invention will be described below with reference to an example.
まず、公知の外科的手段によって人体より抽出された脂
肪細片は若干の蒸留水と混合される。First, fat chips extracted from the human body by known surgical means are mixed with some distilled water.
脂肪細胞は主としてコラーゲンによって形成された外枠
とそれに囲まれた油性成分より成り立っているのである
が、蒸留水の添加により、その浸透圧で脂肪細胞は膨潤
し破壊され油性成分とコラーゲンに分離する。しかし、
外見上はオレンジジュース様に混在している。なお、こ
のとき添加される蒸留水の量は脂肪細胞の量に対して約
２０％程度である。Fat cells mainly consist of an outer frame formed by collagen and an oily component surrounded by it, but when distilled water is added, the osmotic pressure swells and destroys the fat cell, separating it into the oily component and collagen. . but,
From the outside, it looks like orange juice. Note that the amount of distilled water added at this time is about 20% of the amount of fat cells.
実施例では脂肪抽出用の注射器（１０ｃｃ）にあらかじ
め約２ｃｃ程度の蒸留水を入れておいてここに脂肪を抽
出している。In the example, about 2 cc of distilled water is filled in advance in a syringe (10 cc) for fat extraction, and fat is extracted from the syringe.
前記の混在液状成分をフィルターにかけることにより、
該液状成分はａｍ質成分であるゼリー状のコラーゲンと
オイル状の油性成分とにすみやかに分離される。従って
、このとき用いられるフィルターのオープニングの大き
さは、繊維質成分であるコラーゲンを分離するに有効な
大きさのもの、慨ね１００〜５００．程度のものが好ま
しく使用される。フィルターとしては、輸血用器具に使
用されるポリエステル製のろ過網（網の線径は約１ｏｏ
ｕＬ前後、オープニングは２００ｇ程度）が有用である
。By filtering the mixed liquid components mentioned above,
The liquid component is quickly separated into jelly-like collagen, which is an ammonium component, and oily component. Therefore, the opening size of the filter used at this time is generally 100 to 500 mm, which is effective for separating collagen, which is a fibrous component. It is preferable to use one of a certain degree. The filter is a polyester filtration net used for blood transfusion devices (the wire diameter of the net is approximately 10 mm).
Around uL, opening is about 200g) is useful.
フィルターによるろ過に際しては、若干の水圧を加える
ことが効率ヒ奨められる。実施例では前記の注射器から
蒸留水と混合した脂肪分をフィルターに圧送した。When filtering with a filter, it is recommended to apply some water pressure to improve efficiency. In the example, fat mixed with distilled water was pumped into the filter from the syringe described above.
そして、フィルター内に残ったコラーゲンを取り出す。Then, remove the collagen remaining inside the filter.
この方法によって得られたコラーゲン（オートコラーゲ
ン）はそのまま前記した本人に対するコラーゲン注入に
用いることができるが、これを冷凍することにより長期
の保存にたえ必要に応じ随時解凍し使用することも可能
である。The collagen obtained by this method (autocollagen) can be used as is for collagen injection into the person mentioned above, but by freezing it, it can be stored for a long time and can be thawed and used as needed. be.
（作用・効果）
この発明によって得られたコラーゲンは、いわゆるオー
トコラーゲン（自己コラーゲン）として本人へ直接注入
することができる。すなわち、老人性のシワ、傷、にき
び跡等の皮膚の凹陥部分の真皮内に注射器で直接このオ
ートコラーゲンを注入することにより、全くアレルギー
反応の懸念なく、前記凹陥部分を平たん化させ、外見上
の若がえり、傷あとの成形を行なうことができる。(Action/Effect) The collagen obtained by this invention can be directly injected into a person as so-called autocollagen (self-collagen). In other words, by directly injecting this autocollagen into the dermis of skin depressions such as age-related wrinkles, scars, and acne scars, the depressions can be flattened and the appearance improved without any fear of allergic reactions. It can rejuvenate the skin and reshape scars.
さらに、コラーゲンは皮膚の油分２水分を皮膚表面に保
持する働き（モイスチャー効果）があるので、肌のうる
おいを保つ目的でクリーム、乳液あるいは美容液などに
用いることができる。この場合においても、オートコラ
ーゲンであれば、アレルギー等の問題は一切なく、自己
専用の優れた純度の高い化粧品として利用することがで
きる。Furthermore, since collagen has the function of retaining skin oil and moisture on the skin surface (moisture effect), it can be used in creams, milky lotions, beauty essences, etc. for the purpose of keeping the skin moist. Even in this case, autocollagen does not cause any problems such as allergies and can be used as a highly pure cosmetic for personal use.
以上説明したように、この発明によれば、極めて簡単か
つ効率良くコラーゲンを分離することができる。のみな
らず、冷凍保存によって必要に応じて好きな時に使用す
ることが可能である。As explained above, according to the present invention, collagen can be separated extremely easily and efficiently. Not only that, it can be stored frozen and used whenever needed.
Claims (1)
フィルターに通過せしめることによって脂肪細胞の中か
らコラーゲンを分離、取出すことを特徴とするコラーゲ
ンの分離方法。 ２、請求項１におけるフィルターのオープニングの幅が
１００〜５００μであるコラーゲンの分離方法。[Claims] 1. A method for separating collagen, which comprises separating and extracting collagen from fat cells by passing fat fragments extracted from the human body through a filter together with some distilled water. 2. The collagen separation method according to claim 1, wherein the width of the opening of the filter is 100 to 500μ.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11801690A JPH0413700A (en) | 1990-05-08 | 1990-05-08 | Isolation of collagen |
| EP90113376A EP0448770A1 (en) | 1990-03-28 | 1990-07-12 | Method for separating collagen |
| KR1019900014452A KR910016350A (en) | 1990-03-28 | 1990-09-13 | How to separate collagen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11801690A JPH0413700A (en) | 1990-05-08 | 1990-05-08 | Isolation of collagen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0413700A true JPH0413700A (en) | 1992-01-17 |
Family
ID=14725975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11801690A Pending JPH0413700A (en) | 1990-03-28 | 1990-05-08 | Isolation of collagen |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0413700A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005502712A (en) * | 2001-09-14 | 2005-01-27 | マクロポー バイオサージェリー インコーポレイテッド | Preservation of non-embryonic cells derived from non-hematopoietic tissues |
-
1990
- 1990-05-08 JP JP11801690A patent/JPH0413700A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005502712A (en) * | 2001-09-14 | 2005-01-27 | マクロポー バイオサージェリー インコーポレイテッド | Preservation of non-embryonic cells derived from non-hematopoietic tissues |
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