JPH03236771A - Useful microorganism and method for utilization thereof - Google Patents
Useful microorganism and method for utilization thereofInfo
- Publication number
- JPH03236771A JPH03236771A JP2954490A JP2954490A JPH03236771A JP H03236771 A JPH03236771 A JP H03236771A JP 2954490 A JP2954490 A JP 2954490A JP 2954490 A JP2954490 A JP 2954490A JP H03236771 A JPH03236771 A JP H03236771A
- Authority
- JP
- Japan
- Prior art keywords
- positive
- bacillus subtilis
- viable
- properties
- negative
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 17
- 244000005700 microbiome Species 0.000 title abstract description 5
- 238000012360 testing method Methods 0.000 claims abstract description 31
- 239000002689 soil Substances 0.000 claims abstract description 14
- 229920001817 Agar Polymers 0.000 claims abstract description 10
- 239000008272 agar Substances 0.000 claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- 238000003794 Gram staining Methods 0.000 claims abstract description 3
- 229920002472 Starch Polymers 0.000 claims abstract description 3
- 239000008107 starch Substances 0.000 claims abstract description 3
- 235000019698 starch Nutrition 0.000 claims abstract description 3
- 235000015097 nutrients Nutrition 0.000 claims abstract 2
- 235000019645 odor Nutrition 0.000 claims description 51
- 239000003795 chemical substances by application Substances 0.000 claims description 30
- 244000063299 Bacillus subtilis Species 0.000 claims description 22
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 22
- 238000004519 manufacturing process Methods 0.000 claims description 20
- 230000001580 bacterial effect Effects 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 13
- 241001465754 Metazoa Species 0.000 claims description 12
- 230000012010 growth Effects 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000003921 oil Substances 0.000 claims description 9
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 7
- 235000013372 meat Nutrition 0.000 claims description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 claims description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 102000016938 Catalase Human genes 0.000 claims description 3
- 108010053835 Catalase Proteins 0.000 claims description 3
- 229910002651 NO3 Inorganic materials 0.000 claims description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 239000003895 organic fertilizer Substances 0.000 claims description 3
- 239000004475 Arginine Substances 0.000 claims description 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 claims description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 claims description 2
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 2
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 claims description 2
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 claims description 2
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 claims description 2
- 108010048581 Lysine decarboxylase Proteins 0.000 claims description 2
- 102000004316 Oxidoreductases Human genes 0.000 claims description 2
- 108090000854 Oxidoreductases Proteins 0.000 claims description 2
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 claims description 2
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 claims description 2
- 108010046334 Urease Proteins 0.000 claims description 2
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- DLRVVLDZNNYCBX-ZZFZYMBESA-N beta-melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 DLRVVLDZNNYCBX-ZZFZYMBESA-N 0.000 claims description 2
- 230000025938 carbohydrate utilization Effects 0.000 claims description 2
- 239000003925 fat Substances 0.000 claims description 2
- 239000007789 gas Substances 0.000 claims description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 claims description 2
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 claims description 2
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 claims description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 2
- 229960000367 inositol Drugs 0.000 claims description 2
- 238000009630 liquid culture Methods 0.000 claims description 2
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 claims description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 2
- 238000010186 staining Methods 0.000 claims description 2
- 230000001877 deodorizing effect Effects 0.000 abstract description 7
- 210000003608 fece Anatomy 0.000 abstract description 5
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 4
- 230000001603 reducing effect Effects 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 230000001766 physiological effect Effects 0.000 abstract description 3
- 230000000721 bacterilogical effect Effects 0.000 abstract description 2
- 230000000877 morphologic effect Effects 0.000 abstract description 2
- 239000000049 pigment Substances 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 3
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 150000002823 nitrates Chemical class 0.000 abstract 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 25
- 230000000694 effects Effects 0.000 description 12
- 239000006041 probiotic Substances 0.000 description 11
- 235000018291 probiotics Nutrition 0.000 description 11
- 239000000126 substance Substances 0.000 description 10
- 239000002351 wastewater Substances 0.000 description 10
- 230000000529 probiotic effect Effects 0.000 description 9
- 235000019198 oils Nutrition 0.000 description 8
- 238000004065 wastewater treatment Methods 0.000 description 8
- 241000251468 Actinopterygii Species 0.000 description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 241000286209 Phasianidae Species 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 244000291564 Allium cepa Species 0.000 description 2
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 235000005146 Ipomoea eriocarpa Nutrition 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000010813 municipal solid waste Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 241000207782 Convolvulaceae Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 240000001549 Ipomoea eriocarpa Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000009429 distress Effects 0.000 description 1
- 239000003657 drainage water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000019837 monoammonium phosphate Nutrition 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- -1 scum Substances 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 230000019086 sulfide ion homeostasis Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野Ｊ
本発明はバチルス・サブチリスＢＮ１００１（Ｂａｃｉ
ｌｌｕｓ ５ｕｂｔｉｌｉｓ ＢＮｌｏｏＩ）及び含油
排水中のｎヘキサン抽出物質を減じる方法、厨房等の排
水槽にたまるスカムを分解する方法、排水廃物及びトイ
レ等からの悪臭を除去する方法、悪臭の発生を抑制する
方法、浄水化を図る方法、土壌を改善する方法、有機肥
料とする方法、飼料効率の改善を図り、動物の増俸を図
る方法、に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application J The present invention is directed to Bacillus subtilis BN1001 (Bacillus subtilis BN1001).
llus 5ubtilis BNlooI) and n-hexane extracted substances in oil-containing wastewater, a method for decomposing scum that accumulates in drainage tanks such as kitchens, a method for removing bad odors from wastewater waste and toilets, etc., a method for suppressing the generation of bad odors. , methods for purifying water, methods for improving soil, methods for producing organic fertilizers, and methods for improving feed efficiency and increasing animal wages.
【従来の技術ｊ
環境保全の立場から、家庭あるいは各種事業所から排出
される含油排水中のｎヘキサン抽出物質の規制強化が叫
ばれている。また、家庭あるいは各種事業所から排出さ
れる排水廃物、スカム等のとりわけ不快臭を放つものに
対しては、脱臭処理が要求されその処理のために、ある
いはこれから発生するであろう悪臭の防止に、色々なマ
スキング剤等の脱臭剤が市販されている。しかし、ｎヘ
キサン抽出物質の減少、スカムの分解、脱臭、悪臭発生
防止に満足できるものは少ない。特に近年の高午齢化に
伴い高血圧薬あるいは糖尿病薬等を服用する人が増え、
従来の糞尿臭とは異なる臭気うづら養育場その他動物の
糞尿臭も作業者や周辺の住民にとっても苦痛の種である
。また近年、観賞魚等を水槽あるいは池で飼育する人が
増えているが、水槽あるいは池から発する臭気および水
の交換等に悩む人が多い。また植物を愛好する人も多く
、土壌を改善し、きれいな植物を生育したいという要望
もある。また、経済動物を効率的に飼育することができ
ればという要望も多い。[Prior art j] From the standpoint of environmental conservation, there is a call for stricter regulation of substances extracted with n-hexane from oil-containing wastewater discharged from households and various business establishments. In addition, deodorizing treatment is required for wastewater waste, scum, and other substances that emit particularly unpleasant odors discharged from homes or various business establishments. Various deodorizing agents such as masking agents are commercially available. However, there are few methods that are satisfactory in reducing the amount of n-hexane extracted substances, decomposing scum, deodorizing, and preventing the generation of bad odors. In particular, with the aging of the population in recent years, the number of people taking high blood pressure drugs or diabetes drugs has increased.
Odors different from conventional feces and urine odors The feces and urine odors of Uzura nurseries and other animals are also a source of distress for workers and surrounding residents. Furthermore, in recent years, an increasing number of people keep ornamental fish and the like in aquariums or ponds, but many people are concerned about the odor emanating from the aquarium or pond and the problem of changing water. In addition, there are many people who love plants and want to improve the soil and grow beautiful plants. There are also many requests for efficient breeding of commercial animals.
【発明が解決しようとする課題１
家庭あるいは各種事業所から排出される含油排水中のｎ
ヘキサン抽出物質を減らしたいという技術分野、厨房等
の排水槽にたまるスカムを分解しかつこのスカムから発
生する悪臭を消臭したいという技術分野、家庭あるいは
各種事業所から排出される排水廃物等から発する不快な
臭気を軽減あるいはなくしたいという技術分野、これか
ら悪臭発生が予測されるところの悪臭発生の防止を図り
たい技術分野、トイレあるいは家畜を含めた各種動物の
***する糞尿からの臭気を軽減あるいはなくしたいとい
う技術分野、また生ゴミや各種動物の***する糞尿等を
有機肥料として再利用したいという技術分野、観賞魚を
飼育する水槽あるいは池から発する臭気の軽減および浄
水技術分野、土壌を改善したいという技術分野、経済動
物の飼育効率向上を求める技術分野で安全性にすぐれか
つ改善効果を有し満足できるような培養生菌体及び生菌
剤を提供することが本発明の課題である。[Problem to be solved by the invention 1] n in oil-containing wastewater discharged from homes or various businesses
Technical fields that want to reduce hexane extractable substances, technical fields that want to break down scum that accumulates in drainage tanks such as kitchens and deodorize the bad odor generated from this scum, and those that emit from wastewater waste discharged from homes or various businesses. Technical fields that want to reduce or eliminate unpleasant odors, technical fields that want to prevent the occurrence of bad odors in areas where it is predicted that they will occur in the future, and reduce or eliminate odors from toilets or excrement from various animals including livestock. There are technical fields in which you want to reuse food waste and excrement from various animals as organic fertilizer, technical fields in which you want to reduce odors and water purification from aquariums or ponds where ornamental fish are raised, and fields in which you want to improve soil. It is an object of the present invention to provide a cultured viable bacterial cell and a viable bacterial agent that are excellent in safety, have an improvement effect, and are satisfactory in the technical field and in the technical field that seeks to improve the efficiency of raising economic animals.
［課題を解決するための手段］
本発明者らは、含油排水中のｎヘキサン抽出物質を減す
る微生物を発見すべく、各地域の土壌より微生物のスク
リーニングを実施した結果、バチルス属に属する新しい
曹を発見し、これをバチルス・サブチリスＢＮ１００１
と命名した。[Means for Solving the Problems] In order to discover microorganisms that reduce n-hexane extractable substances in oil-containing wastewater, the present inventors screened soil in various regions for microorganisms, and as a result, discovered a new microorganism belonging to the genus Bacillus. I discovered Cao and called it Bacillus subtilis BN1001.
It was named.
このバチルス・サブチリスＢＮ１００１の培養生菌体を
含油排水で試験するうちに減油効果の他に脱臭効果を発
見し、家庭あるいは各種事業所から排出される排水廃物
とりわけ不快臭を発する所からの脱臭効果を繰返し確認
した。さらにトイレや各種動物の糞尿の臭気も不快を覚
えない程に軽減すること、生ゴミ等これから悪臭の発生
が予測されるところで悪臭発生が抑制されること、各種
動物の糞尿は有機肥料として再利用できること、観賞魚
飼育水槽や池での魚臭および浄水効果も発見した。さら
に土壌に入れよく混ぜた後、植物の種を蒔いたところそ
の生育の良さを発見、また各種経済動物の飼料に添加し
た結果、飼料利用効率の向上、動物の増体が図られるこ
とをも発見した。While testing the cultured live bacteria of Bacillus subtilis BN1001 on oil-containing wastewater, we discovered that in addition to the oil-reducing effect, it also has a deodorizing effect, which can be used to deodorize wastewater discharged from homes and various businesses, especially from areas that emit unpleasant odors. The effect was confirmed repeatedly. Furthermore, the odor of toilets and the excrement of various animals is reduced to a level that does not cause discomfort, the occurrence of bad odors is suppressed in areas where odor is predicted to occur in the future, such as garbage, and the excrement and urine of various animals are reused as organic fertilizer. We also discovered that it can reduce fish odor and purify water in aquarium fish tanks and ponds. Furthermore, when we sowed plant seeds in the soil and mixed them well, we discovered that they grew well, and we also found that adding them to the feed of various economical animals improved feed utilization efficiency and increased the number of animals. discovered.
このよう９な新しい知見を得、更に繰返し試験した結果
、本発明が完成されたのである。As a result of these nine new discoveries and repeated tests, the present invention was completed.
すなわち、本発明はバチルス・サブチリスＢＮ１００１
の培養生菌体及び生菌剤に関する。That is, the present invention relates to Bacillus subtilis BN1001
This invention relates to cultured viable bacterial cells and viable bacterial agents.
本発明の菌株は、本発明者らが土壌から新たに分離した
バチルス・サブチリスＢＮ１００１であり、本菌の細菌
学的性状は次の通りである。The strain of the present invention is Bacillus subtilis BN1001, which was newly isolated by the present inventors from soil, and the bacteriological properties of this bacteria are as follows.
Ａ、形態的性質
（１） 肉汁寒天で培養した栄餐細胞は、０．５〜０
．７Ｘ １−５〜３．０μｍのかん菌（２）グラム染色
は陽性
（３） ３０℃２〜３日の培養で内生胞子を形成、０
．５〜０．７Ｘ Ｏ，８〜ｌ−５μｍの長円形（４）胞
子は胞子のうのほぼ中央に位置し、胞子のうの膨らみは
殆ど認められない
（５）周毛により運動
（６）抗酸性染色は陰性
Ｂ、培養的性質
（１）普通寒天培養：３０〜４５℃、１〜３日の培養で
よく生育する
（２）肉汁寒天培養：生育は良好で集落は茶色クリーム
様を呈し、拡散
性色素は生産しない
（３）肉汁液体培養：液面に白色の曹膜を形成する
（４）肉汁ゼラチン穿刺培養二層状に液化され（５）ス
キムミルク培養：アルカリ性を呈し、ペプトン化される
Ｃ０生理的性質
（１） 硝酸塩の還元性：陽性
（２）脱芽反応：陰性
（３）ＶＰ反応：陽性
（４）ＭＲテスト：陰性
（５）スターチの加水分解性：陽性
（６）硝酸塩およびアンモニウム塩を利用する（７）色
素の生産：顕著な水溶性および非水溶性色素を生成しな
い
（８）
（９）
（ｌＯ）
（１１）
（１２）
（１３）
（１４）
（１５）
カタラーゼ：陽性
クエン酸の利用性：陽性
アルギニンの分解性：陰性
インドールの生産：陰性
硫化水素の生成：陰性
オキシダーゼ：陽性
ウレアーゼ：陰性
リジンデカルボキシラーゼ：擬陽性
（１６）耐食塩性２１０％食塩添加肉汁寒天培地上で生
育する
（１７）生育温度＝２５〜４５℃の温度範囲でよく生育
するが、５５℃では生育しな
い
（１８）嫌気条件下での生育は認められない（１９）Ｏ
Ｆテスト二〇型
（２０）ヒューレイフソン培地による糖の利用性：Ｄ−
グルコース、Ｌ−アラビノース、Ｄ−キシロース、Ｄ−
マンニトール、ソルビトール、シュークローズからの酸
の生成が認められるが、ガスの発生は認められない
イノシトール、ラムノース、メリビオース、ラフィノー
スからの酸とガスの生成は認められない
以上の菌学的性質を有するＢＮ１００１株をバーギイズ
・マニュアル・オプ・システマチイック・バクテリオク
ジ−１９８６午版（Ｂｅｒｇｅｙ’ｓ Ｍａｎｕａｌｏ
ｒ Ｓｙｓｔｅｍａｔｉｃ Ｂａｃｔｅｒｉｏｌｏｇｙ
、１９８６）に記載された既知菌種と比較し、好気性の
グラム陽性かん口で内生胞子を作り、カタラーゼ陽性で
あることから本株はバチルス属に所属すると判定、更に
胞子と胞子のうの形態および各種の生理的性質よりバチ
ルス・サブチリスに所属すると同定した。しかし、典型
的なバチルス・サブチリスとはｎヘキサン抽出物質の低
減量化、スカムの分解、脱臭、浄水化、土壌改善化、飼
料効率化、増俸促進化等にすぐれた有用性を示す点で異
なっており、新曹株とした。 本曹株は、工業技術院微
生物工業技術研究所に微工研曹寄第１１１３２号（ＦＥ
ＲＭ Ｐ−１１１３２）として寄託されている。A. Morphological properties (1) The eutrophic cells cultured in meat juice agar are 0.5 to 0.
．． 7X 1-5 to 3.0 μm rods (2) Gram staining is positive (3) Forms endospores after 2 to 3 days of culture at 30°C, 0
．． 5-0.7X O, 8-1-5 μm oval shape (4) The spore is located almost in the center of the sporangium, and there is almost no swelling of the sporangium (5) It is motile due to peritial hairs (6) Acid-fast staining is negative B.Cultural properties (1) Ordinary agar culture: Grows well in culture for 1-3 days at 30-45°C. (2) Juice agar culture: Growth is good, colonies exhibit a brown cream-like appearance. , does not produce diffusible pigments (3) Meat juice liquid culture: Forms a white soda film on the liquid surface (4) Meat juice gelatin puncture culture liquefies into two layers (5) Skim milk culture: exhibits alkalinity and becomes peptonized C0 physiological properties (1) Reducibility of nitrate: Positive (2) De-sprouting reaction: Negative (3) VP reaction: Positive (4) MR test: Negative (5) Hydrolyzability of starch: Positive (6) Nitrate and (7) Production of dyes using ammonium salts: does not produce significant water-soluble and water-insoluble dyes (8) (9) (lO) (11) (12) (13) (14) (15) Catalase: Positive citric acid utilization: Positive arginine degradability: Negative Indole production: Negative Hydrogen sulfide generation: Negative oxidase: Positive Urease: Negative Lysine decarboxylase: False positive (16) Salt resistance On 210% salt-added meat juice agar medium (17) Grows well in the growth temperature range of 25 to 45°C, but does not grow at 55°C (18) Growth is not observed under anaerobic conditions (19) O
F test type 20 (20) Sugar utilization by Huleifson medium: D-
Glucose, L-arabinose, D-xylose, D-
Production of acid and gas from inositol, rhamnose, melibiose, and raffinose is not observed. BN1001 has superior mycological properties. Bergey's Manual of Systematic Bacteriology - 1986 Edition
r Systematic Bacteriology
, 1986), this strain was determined to belong to the genus Bacillus because it produced endospores in an aerobic Gram-positive mouth and was positive for catalase. It was identified as belonging to Bacillus subtilis based on its morphology and various physiological properties. However, it differs from the typical Bacillus subtilis in that it is highly useful in reducing the amount of n-hexane extracted substances, decomposing scum, deodorizing, purifying water, improving soil, improving feed efficiency, and promoting increased wages. Therefore, it was designated as Shinso stock. The Honso strain was submitted to the Institute of Microbial Technology, Agency of Industrial Science and Technology, with the number 11132 (FE).
RM P-11132).
［実施例１
以下、本菌の製造例および得られた培養生菌体、生菌剤
を使用した試験例を示すが、本発明はこれらに限定され
たものではない。[Example 1] Hereinafter, a production example of the present bacteria and a test example using the obtained cultured viable cells and a viable agent will be shown, but the present invention is not limited thereto.
製造例 ｌ 水道水１５Ｌにグルコース７５ｇ１肉エキス１０５ｇ。Manufacturing example l 15L of tap water, 75g of glucose, 105g of meat extract.
ペプトン１５０ｇ、塩化ナトリウム４５ｇを溶解させ、
ｐｉｔを７．２に調整した培地を３ＯＬジャーファーメ
ンタ−に入れ、１２１’０３０分間殺菌し、冷却後これ
にあらかじめ前培養しておいたバチルス・サブチリスＢ
Ｎ１００１の培養液を接種し、３０°０２４時間通気撹
はん培養した。Dissolve 150g of peptone and 45g of sodium chloride,
The medium adjusted to a pit of 7.2 was placed in a 3OL jar fermenter, sterilized for 121'030 minutes, and after cooling, Bacillus subtilis B, which had been pre-cultured in this, was added.
A culture solution of N1001 was inoculated and cultured with aeration at 30°C for 24 hours.
このようにして得た培養液をバチルス・サブチリスＢＮ
１００１の培養生菌体としても利用できる。が、この培
養液にパーライトを加えて混合し、更にこれを乾燥後粉
砕することにより、Ｉ（チルス・サブチリスＢＮ１００
１の生菌剤１．３ｋｇが得られる。この生菌剤に含まれ
る生曹数はｌ×１０’個／ｇであった。The culture solution obtained in this way was used as Bacillus subtilis BN.
It can also be used as a cultured live bacterial cell of 1001. However, by adding perlite to this culture solution, mixing it, drying it and pulverizing it, I (Tilus subtilis BN100
1.3 kg of viable bacterial agent of No. 1 is obtained. The number of raw soda contained in this probiotic agent was 1×10' pieces/g.
製造例 ２
市販大豆粕１ｋｇにｐＨを７に調整したコーンステイー
プリカー（Ｃ５Ｌ） ５００ｇ、水道水３００ｇを加え
、よく混合し、１２１’ｃ！６０分間殺菌し、予め前培
養しておいたバチルス・サブチリスＢＮ１００１の培養
液を接種し、３０″Ｃ！１２０時間培養した。このよう
にして得られた培養物に炭酸カルシウムを混合してバチ
ルス・サブチリスＢＮ１００１の生菌剤１．６ｋｇを得
た。この生菌剤に含まれる生箇数はｌＸｌ０’個／ｇで
あった。Production Example 2 Add 500 g of corn staple liquor (C5L) whose pH has been adjusted to 7 and 300 g of tap water to 1 kg of commercially available soybean meal, mix well, and make 121'c! The culture solution of Bacillus subtilis BN1001, which had been sterilized for 60 minutes and pre-cultured, was inoculated and cultured for 120 hours at 30"C.The thus obtained culture was mixed with calcium carbonate to inoculate Bacillus subtilis BN1001. 1.6 kg of a viable bacterial agent of Subtilis BN1001 was obtained.The number of viable bacteria contained in this viable agent was 1X10' cells/g.
試験例 ｌ
コーン油１０００ ｐ ｐ ｍ、酵母エキス０．３％、
リン酸二水素アンモニウム０．１％、塩化カリウム０．
０２％、硫酸マグネシウム０．０２％、ｐＨ７，８の油
脂培地に製造例１で調製したバチルス・サブチリスＢＮ
Ｉ００１の生菌剤を入れ、３０℃で２２時時間上う反応
させ、ｎヘキサン抽出物質の量を測定した。その結果を
第１表に示す。Test example l Corn oil 1000 ppm, yeast extract 0.3%,
Ammonium dihydrogen phosphate 0.1%, potassium chloride 0.
Bacillus subtilis BN prepared in Production Example 1 in an oil medium containing 0.02% magnesium sulfate, 0.02% magnesium sulfate, and pH 7.8.
A viable bacterial agent of I001 was added and allowed to react at 30°C for 22 hours, and the amount of n-hexane extracted material was measured. The results are shown in Table 1.
第１表
試験例 ２
各種油脂について試験例１に記述した方法で試験し、減
少量を試験開始時のｎヘキサン抽出物質量で除し、その
比率を油脂分解力として第２表に示す。Table 1 Test Example 2 Various oils and fats were tested by the method described in Test Example 1, the amount of reduction was divided by the amount of n-hexane extracted material at the start of the test, and the ratio is shown in Table 2 as the oil and fat decomposition power.
第２表
試験例 ３
菓子工場の排水（ｊｉｌ（水）をサンプリングし、製造
例１で調製したバチルス・サブチリスＢＮ１００１の生
菌剤を入れ、３０℃で２２時時間上う反応させ、ｎヘキ
サン抽出物質の量を測定した。その結果を第３表に示す
。Table 2 Test Example 3 Drainage water from a confectionery factory was sampled, Bacillus subtilis BN1001 probiotic prepared in Production Example 1 was added, reacted at 30°C for 22 hours, and extracted with n-hexane. The amount of substance was measured and the results are shown in Table 3.
第３表
試験例 ４
中華レストランの排水処理槽（６トン容量）に製造例１
で得た生菌剤を初回に５０ ｐ ｐ ｒｎ Ｎ以後２週
間毎に５０ｐｐｍを、さらに調理場の流しより毎日５ｇ
づつを投入し、固化したスカムの変化をみた。その結果
、２週間後には、液面が黒ずみ、やや軟化の状態を示し
、４週間後には、明らかに軟化、上から棒で押すと液面
が見える程になった。Table 3 Test Example 4 Manufacturing Example 1 in a Chinese restaurant wastewater treatment tank (6 ton capacity)
50 ppm of the viable bacterial agent obtained in
I added a small amount of scum and watched the changes in the solidified scum. As a result, after 2 weeks, the liquid surface had turned dark and was slightly softened, and after 4 weeks, it had clearly softened to the point that the liquid level was visible when pressed from above with a stick.
なお、生菌側投入前には、スカム周辺から悪臭が発して
いたが、２週間後には消臭した。３力月後には、スカム
の固化した状態はなくなった。Note that a bad odor was emitted from around the scum before the live bacteria side was added, but the odor disappeared after two weeks. After three months, the solidified state of the scum disappeared.
試験例 ５
ファミリーレストランの排水処理槽（１２トン容量の合
併槽）に製造例１で調製した生菌剤を試験例４と同じ方
法で投入した。試験例４と同じくスカムは固化しており
上から棒でつついても割れず、水をかけても軟化し難い
状態を示していた。更に悪臭がひどく、周辺より苦情が
多く、レストランの関係者は困っていた。Test Example 5 The probiotic agent prepared in Production Example 1 was poured into a wastewater treatment tank (combined tank with a capacity of 12 tons) of a family restaurant in the same manner as in Test Example 4. As in Test Example 4, the scum was solidified and did not break even when poked from above with a stick, and was difficult to soften even when water was poured over it. Furthermore, the odor was so bad that there were more complaints than in the surrounding area, and the restaurant staff was in trouble.
生菌剤の投入を開始して、排水処理槽のスカムの状態の
変化、放流水の透視度および処理槽およびその周辺の臭
気を観察した。水の透視度は透視計を使用、臭気はポー
タプル型ニオイセンサーｘｐ−３２９型（新コスモス電
気株式会社製）を使用、スカムの状態は肉眼観察した。After starting the injection of viable bacteria, we observed changes in the state of the scum in the wastewater treatment tank, the visibility of the effluent water, and the odor in and around the treatment tank. The transparency of the water was measured using a fluoroscope, the odor was measured using a portaple odor sensor model XP-329 (manufactured by Shin Cosmos Electric Co., Ltd.), and the state of the scum was observed with the naked eye.
なお現場試験に先立ち、不快臭の強い排水を排水処理槽
からサンプリングし、これを三角７ラスコに入れ、製造
例１で得た生菌剤を投入したものとしないものについて
ｎヘキサン抽出物質の量及び臭気の測定を実施した。な
お生菌剤の投入は試験開始時のみとし、３０°Ｃで振と
う反応した。フラスコでの試験結果を第４表に、現場試
験での結果を第５表に示しｔこ。Prior to the on-site test, wastewater with a strong unpleasant odor was sampled from the wastewater treatment tank and placed in a triangular 7 ladle, and the amount of n-hexane extracted material was measured with and without the probiotic agent obtained in Production Example 1. and odor measurements were conducted. The viable bacterial agent was added only at the start of the test, and the shaking reaction was carried out at 30°C. Table 4 shows the results of the flask test, and Table 5 shows the results of the field test.
臭気の測定
ニオイセンサーは排水処理槽の風上から５〜１０数ｍ離
れた不快な臭気を感じないところでの臭気を１００に調
整して、排水処理槽とその付近の臭気を測定した。この
数値が高い程、不快臭の強いことを意味し、単位はない
。Odor measurement The odor sensor was adjusted to 100 at a location 5 to 10 meters away from the windward of the wastewater treatment tank where no unpleasant odor was detected, and the odor in the wastewater treatment tank and its vicinity was measured. The higher the value, the stronger the unpleasant odor, and there is no unit.
１３０〜１５０の数値レベル：やや臭気を感じる程度２
００〜２５０の数値レベル：臭気として誰もが認める程
度
３００以上の数値レベル ：誰もが不快感を訴えるほど
の臭気
第４表 ７アミリーレストランでの悪臭水抜取り試験結
果ファミリーレストランやホテル等の食堂厨房施設で利
用できる。Numerical level of 130 to 150: Slight odor level 2
Numerical level from 00 to 250: Everyone recognizes it as an odor Numerical level of 300 or above: An odor so strong that anyone complains of discomfort It can be used in the cafeteria kitchen facilities.
第５表 ７アミリーレストランでの現場試験結果第４表
に示す通り、生菌剤を投入した区では、臭気、ｎヘキサ
ン抽出物質の量が減っている。第５表の現場試験結果も
フラスコ試験の結果を裏付けている。即ち、生菌剤を投
入することにより、透視度の向上が図られると同時に悪
臭が抑制され、かつｎヘキサン抽出物質の量も減じるこ
とから、臭気の単位は、ない（周辺との比較値である。Table 5 Field test results at Amily Restaurant 7 As shown in Table 4, the odor and amount of n-hexane extracted substances were reduced in the areas where the probiotics were added. The field test results in Table 5 also support the flask test results. In other words, by adding a probiotic agent, the transparency is improved and at the same time, the odor is suppressed, and the amount of n-hexane extracted substances is also reduced, so there is no unit of odor (compared to the surrounding area). be.
臭気の測定参照）試験例６
うづら場から排出される糞１ｋｇに製造例１の生菌剤５
ｇを入れ、１力月後の臭気を試験例１に示した方法で測
定した結果、試験開始時４５０あった臭気が１３０と減
っていた。(See odor measurement) Test Example 6 Add 5 of the viable bacterial agent of Production Example 1 to 1 kg of feces discharged from the quail farm.
As a result of measuring the odor after one month using the method shown in Test Example 1, the odor had decreased from 450 at the start of the test to 130.
また、うづら場現場にて糞ｌトンあたり５ｇを毎日投入
、さらに１週間毎に２５０ｇを投入し、２力月経過後臭
気を測定した結果、試験開始時２８０あった臭気が１３
０と減り、激臭の効果を認め、うづら場及びこれと類似
する養豚場、養鶏場ほかでの利用が考えられる。In addition, 5g per ton of feces was added every day at the quail farm site, and 250g was added every week, and the odor was measured after 2 months.
Since the odor was reduced to 0 and the strong odor effect was recognized, its use in quail farms and similar pig farms, poultry farms, etc. is considered.
試験例 ７
観賞魚水槽（６０Ｌ容量）に製造例１の生菌剤２０〜６
０ｍ ｇを毎日投入した結果、投入まもなく魚臭が消失
、さらに水槽の水の交換も約１〜２週間遅らせることが
できた。なお、魚が本生菌剤の投入により死ぬというこ
とはなかった。即ち、家庭あるいは養魚場での利用が可
能である。Test Example 7 Probiotic agent 20 to 6 of Production Example 1 was placed in an aquarium fish tank (60L capacity)
As a result of adding 0 mg daily, the fishy odor disappeared shortly after adding it, and it was also possible to delay changing the water in the aquarium for about 1 to 2 weeks. It should be noted that no fish died due to the injection of this live bacterial agent. That is, it can be used at home or at a fish farm.
試験例 ８
家庭から排出される生ゴミに製造例１の生菌剤３〜５ｇ
を就寝前に入れた結果、翌朝には誰もが気付かない程に
消臭した。同じ現象を不快臭を発している流し、風呂場
、洗濯機置場、トイレでも効果を確認した。また、流し
や風呂場でのヌルヌルした状態がなくなり、家庭での既
に悪臭が発生している場所あるいはこれから悪臭が発生
すると予想される所での利用が可能である。Test Example 8 3 to 5 g of the viable bacterial agent of Production Example 1 was added to household garbage.
As a result of putting it on before going to bed, the odor was gone to the extent that no one noticed by the next morning. The same effect was confirmed in sinks, bathrooms, washing machine areas, and toilets that emit unpleasant odors. In addition, it eliminates the slimy state in sinks and bathrooms, and can be used in places in the home where bad odors are already occurring or where bad odors are expected to occur in the future.
試験例 ９
製造例１の生菌剤３〜５ｇを土壌５００ｇに混合したも
のとしないものに、朝顔の種を蒔き、その成長を比較し
た結果、生菌剤を入れた方が無添加区に比べて約６〜ｌ
Ｏ％長く生長し、得られた種子の数は約１割多かった。Test Example 9 As a result of sowing morning glory seeds with and without 500 g of soil mixed with 3 to 5 g of the probiotic agent from Production Example 1 and comparing their growth, it was found that those with the probiotic agent were better in the non-additive area. Approximately 6~l compared to
It grew 0% longer and the number of seeds obtained was about 10% higher.
また、臭気の強い土壌に製造例工の生菌剤を混ぜあわせ
た結果、１〜２日して臭気は消失、この土壌にネギを植
え一般土壌と比較した結果、朝顔同様、ネギの生長に約
５％程の差を認め、収穫量の増大が図れる結果を得た。In addition, when we mixed soil with a strong odor with the probiotics produced by the production example, the odor disappeared in 1 to 2 days.As a result of planting green onions in this soil and comparing it with ordinary soil, we found that the growth of green onions was similar to that of morning glories. A difference of about 5% was observed, and the results showed that the yield could be increased.
試験例 ｌＯ
製造例２の生菌剤５０ｐｐ重量を市販ブロイラー前期用
（くみあい飼料）の飼料に添加したものとしないものを
準備し、生後２〜３日のブロイラーに給与して成長試験
を実施した。結果は第６表に示す通りである。Test Example 1O A growth test was conducted by adding and not adding 50pp of the viable bacterial agent of Production Example 2 to feed for commercially available early-stage broilers (pumping feed) and feeding it to broilers 2 to 3 days old. . The results are shown in Table 6.
８６表
とにより、酸油効果、脱臭効果および浄水効果を奏し、
さらに土壌に混合することにより植物生長促進効果、有
機肥料化の改善効果を、さらに動物に投与することによ
り、増俸効果、飼料効率の改善効果といった多機能の広
範かつ卓越した効果を奏するものである。Table 86 shows acid oil effect, deodorizing effect and water purification effect,
Furthermore, when mixed with soil, it has the effect of promoting plant growth and improving organic fertilizers, and when administered to animals, it has a wide range of multifunctional and outstanding effects such as increasing wages and improving feed efficiency. be.
また本発明は、副作用のない安全な排水廃物処理剤とし
て、あるいは飼料添加剤として環境改善、排水廃物処理
、農業、畜産業、水産業といった各技術分野において重
要な役割を果たすものである。Furthermore, the present invention plays an important role in various technical fields such as environmental improvement, wastewater treatment, agriculture, livestock farming, and fisheries as a safe wastewater treatment agent without side effects or as a feed additive.
第６表に示すように、本発明の生菌剤を添加した配合飼
料を給与することにより、大幅な増体効果がみられると
ともに飼料要求率も改善することができた。As shown in Table 6, by feeding the mixed feed containing the probiotic agent of the present invention, a significant weight gain effect was observed and the feed conversion rate was also improved.
［発明の効果］[Effect of the invention]
Claims (1)
ＢＮ１００１（ＢａｃｉｌｌｕｓｓｕｂｔｉｌｉｓＢＮ
１００１） （１）かん菌 （２）グラム染色：陽性 （３）内生胞子を形成、長円形 （４）運動性あり （５）抗酸性染色：陰性 （６）普通寒天培養：よく生育 （７）肉汁寒天培養：生育は良く、集落は茶色クリーム
様 （８）肉汁液体培養：液面に白色の菌膜を形成 （９）硝酸塩の還元性：陽性 （１０）ＶＰ反応：陽性 （１１）スターチの加水分解性：陽性 （１２）カタラーゼ：陽性 （１３）クエン酸の利用性：陽性 （１４）アルギニンの分解性：陰性 （１５）インドールの生成：陰性 （１６）硫化水素の生成：陰性 （１７）オキシダーゼ：陽性 （１８）ウレアーゼ：陰性 （１９）リジンデカルボキシラーゼ：擬陽性 （２０）耐食塩性：１０％食塩添加肉汁寒天培地上で生
育 （２１）生育温度：２５〜４５℃の温度範囲でよく生育 （２２）ＯＦテスト：Ｏ型 （２３）ヒューレイフソン培地による糖の利用性：Ｄ−
グルコース、Ｌ−アラビノース、Ｄ−キシロース、Ｄ−
マンニトール、ソルビトール、シュークローズからの酸
の生成が認められるが、ガスの発生は認められない イノシトール、ラムノース、メリビオース、ラフィノー
スからの酸とガスの生成は認められない （２）バチルス・サブチリスＢＮ１００１の培養生菌体
及び生菌剤を用いて油脂を分解する方法 （３）バチルス・サブチリスＢＮ１００１の培養生菌体
及び生菌剤を用いて悪臭を除去する方法 （４）バチルス・サブチリスＢＮ１００１の培養生菌体
及び生菌剤を用いて悪臭の発生を抑制 （５）バチルス・サブチリスＢＮ１００１の培養生菌体
及び生菌剤を用いて富栄養水の濁りを除去する方法 （６）バチルス・サブチリスＢＮ１００１の培養生菌体
及び生菌剤を用いて動物の増体を図る方法 （７）バチルス・サブチリスＢＮ１００１の培養生菌体
及び生菌剤を用いて土壌を改善する方法 （８）バチルス・サブチリスＢＮ１００１の培養生菌体
及び生菌剤を用いて有機肥料を調製する方法Scope of Claims: (1) Bacillus subtilis BN1001 having the following mycological properties:
1001) (1) Bacteria (2) Gram staining: positive (3) Forming endospores, oval (4) Motile (5) Acid-fast staining: negative (6) Ordinary agar culture: Good growth (7 ) Meat juice agar culture: Good growth, brown cream-like colonies (8) Meat juice liquid culture: White bacterial film formed on the liquid surface (9) Nitrate reducibility: Positive (10) VP reaction: Positive (11) Starch Hydrolyzability of: Positive (12) Catalase: Positive (13) Utilization of citric acid: Positive (14) Degradability of Arginine: Negative (15) Production of indole: Negative (16) Production of hydrogen sulfide: Negative (17 ) Oxidase: Positive (18) Urease: Negative (19) Lysine decarboxylase: False positive (20) Salt resistance: Growth on broth agar medium supplemented with 10% salt (21) Growth temperature: Good in the temperature range of 25 to 45°C Growth (22) OF test: O type (23) Sugar utilization by Huleifson medium: D-
Glucose, L-arabinose, D-xylose, D-
Production of acid and gas from inositol, rhamnose, melibiose, and raffinose is not observed (2) Cultivation of Bacillus subtilis BN1001 Method for decomposing fats and oils using viable bacteria and a viable agent (3) Cultivation of Bacillus subtilis BN1001 Method for removing bad odors using viable bacteria and a viable agent (4) Culture of Bacillus subtilis BN1001 (5) Cultivation of Bacillus subtilis BN1001 Method for removing turbidity of nutrient-rich water using live bacteria and a viable bacterial agent (6) Cultivation of Bacillus subtilis BN1001 Method for increasing the number of animals using viable bacteria and a viable agent (7) Cultivation of Bacillus subtilis BN1001 Method for improving soil using viable bacteria and a viable agent (8) Cultivation of Bacillus subtilis BN1001 Method for preparing organic fertilizer using viable bacteria and viable agent
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2029544A JP2553727B2 (en) | 1990-02-13 | 1990-02-13 | Useful microorganisms and their use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2029544A JP2553727B2 (en) | 1990-02-13 | 1990-02-13 | Useful microorganisms and their use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03236771A true JPH03236771A (en) | 1991-10-22 |
| JP2553727B2 JP2553727B2 (en) | 1996-11-13 |
Family
ID=12279068
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2029544A Expired - Lifetime JP2553727B2 (en) | 1990-02-13 | 1990-02-13 | Useful microorganisms and their use |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2553727B2 (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH072613A (en) * | 1993-04-22 | 1995-01-06 | Natl Fedelation Of Agricult Coop Assoc | Bacterial preparation for agricultural purposes |
| KR20000007270A (en) * | 1998-07-01 | 2000-02-07 | 티엔 웨 이첸 | Process for producting water soluble amino acid by using bacteria strain which can decompose aquatic waste |
| WO2000042169A1 (en) * | 1999-01-14 | 2000-07-20 | Gold Kosan Co. Ltd. | Novel microorganism and use thereof |
| JP2001129580A (en) * | 1999-11-02 | 2001-05-15 | Kl Plant Kk | Apparatus for treating waste water containing oil and fat |
| JP5308570B1 (en) * | 2012-10-12 | 2013-10-09 | 昭司 野口 | Method for urination treatment of livestock animals |
| WO2016031804A1 (en) * | 2014-08-25 | 2016-03-03 | 株式会社日本環境科学研究所 | Composition, support, wastewater treatment system, wastewater treating method, deodorization method, and batch wastewater treating method |
| WO2019220831A1 (en) | 2018-05-17 | 2019-11-21 | シーシーアイホールディングス株式会社 | Novel microorganism for degrading fats and oils |
| WO2019244263A1 (en) * | 2018-06-19 | 2019-12-26 | 株式会社日本環境科学研究所 | Wastewater treatment system |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8268608B2 (en) | 2006-03-31 | 2012-09-18 | Menicon Co., Ltd. | Method of treating biomass, compost, mulching material for livestock and agent for treating biomass |
| CN103058385B (en) * | 2011-10-20 | 2014-05-21 | 中国水产科学研究院渔业机械仪器研究所 | Process for treatment of high density aquaculture sewage |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH072613A (en) * | 1993-04-22 | 1995-01-06 | Natl Fedelation Of Agricult Coop Assoc | Bacterial preparation for agricultural purposes |
| KR20000007270A (en) * | 1998-07-01 | 2000-02-07 | 티엔 웨 이첸 | Process for producting water soluble amino acid by using bacteria strain which can decompose aquatic waste |
| WO2000042169A1 (en) * | 1999-01-14 | 2000-07-20 | Gold Kosan Co. Ltd. | Novel microorganism and use thereof |
| US6812022B1 (en) | 1999-01-14 | 2004-11-02 | Gold Kosan Co., Ltd. | Bacillus subtilis takemi and compositions thereof |
| JP2001129580A (en) * | 1999-11-02 | 2001-05-15 | Kl Plant Kk | Apparatus for treating waste water containing oil and fat |
| JP5308570B1 (en) * | 2012-10-12 | 2013-10-09 | 昭司 野口 | Method for urination treatment of livestock animals |
| WO2016031804A1 (en) * | 2014-08-25 | 2016-03-03 | 株式会社日本環境科学研究所 | Composition, support, wastewater treatment system, wastewater treating method, deodorization method, and batch wastewater treating method |
| JPWO2016031804A1 (en) * | 2014-08-25 | 2017-06-08 | 株式会社日本環境科学研究所 | Composition, carrier, wastewater treatment system, wastewater treatment method, deodorization method, and batch-type wastewater treatment method |
| US10052399B2 (en) | 2014-08-25 | 2018-08-21 | Japan Environmental Science Company | Composition, support, wastewater treatment system, wastewater treating method, deodorization method, and batch wastewater treating method |
| WO2019220831A1 (en) | 2018-05-17 | 2019-11-21 | シーシーアイホールディングス株式会社 | Novel microorganism for degrading fats and oils |
| US11897799B2 (en) | 2018-05-17 | 2024-02-13 | Cci Holdings Inc. | Microorganism for degrading oils and fats |
| WO2019244263A1 (en) * | 2018-06-19 | 2019-12-26 | 株式会社日本環境科学研究所 | Wastewater treatment system |
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| Publication number | Publication date |
|---|---|
| JP2553727B2 (en) | 1996-11-13 |
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