CN109279748A - A kind of pig farm microbial deodorant and preparation method - Google Patents
A kind of pig farm microbial deodorant and preparation method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
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Abstract
The invention discloses a kind of pig farm microbial deodorant and preparation methods, the preparation method comprises the following steps: stalk is ground into stalk powder by (1)；Anoxybiotic calcination, cooling obtain charcoal；(2) bacillus after activation, the lactic acid bacteria after activation and the saccharomycete after activation are inoculated into corresponding fluid nutrient medium, cultivate, respectively obtains content > 109The bacterium solution of CFU/mL；Mixing, obtains composite bacteria liquid；(3) charcoal is uniformly mixed with composite bacteria liquid, is stirred, culture；(4) it is separated by solid-liquid separation, solid drying obtains a kind of pig farm microbial deodorant.A kind of pig farm microbial deodorant of the invention, energy secretion activity substance promote beneficial microbe growth, generate fragrance；So that organic matter is fermented, adjust pH, inhibits harmful bacteria growth；Enzyme is generated, the generation of foul gas is inhibited.Environment-friendly high-efficiency of the present invention, at low cost, preparation method is easy, environmental protection.
Description
Technical field
The invention belongs to technical field of animal husbandry, it is specifically related to a kind of pig farm microbial deodorant and preparation side
Method.
Background technique
Currently, intensive culture emerges in multitude with the development of various regions pastoral industry, the discharge amount of excrement is more and more,
The pollution problem of excrement becomes increasingly conspicuous.The quality of pig manure is thinner, and ingredient is more complex, contains protein, fats, organic acid, fiber
Element, hemicellulose and inorganic salts.Due to the features such as pig manure pathogen is more, cause of disease harm is serious, generated as well as pig manure
Foul smell directly affect human and livestock health, be also degrading the working environment that resource utilization operator is engaged in Collection utilization, thus
It is the type that resource utilization degree is minimum in livestock and poultry cultivation night soil.
Stink odor caused by pig manure is complex, including indoles organic acid, scatol, fats amine mercaptan, sulphur
Change hydrogen, ammonia etc., wherein maximum with the harm of hydrogen sulfide and ammonia.Removal pig manure foul smell available physical, chemistry and biology at present
Method is handled.Physical deodorization method mainly has WATER-WASHING METHOD, absorption method, screening agent method etc., and chemical deodorization method mainly has direct combustion
Burning method, chemical oxidization method, high-temperature oxidation etc., biological odor removal method are to be decomposed using microorganism, convert stink odor to reach
Deodorization purpose, therefore also it is microbial deodorant method.Effect using the method deodorization of physics and chemistry is preferable, but there is investment fortune
The problems such as row is at high cost, and secondary pollution is serious.Therefore, new low cost, with good application value and environment protection significance
The technical issues of method of microbial deodorant is urgent need to resolve.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of pig farm microbial deodorant of low cost.
A second object of the present invention is to provide a kind of preparation methods of pig farm microbial deodorant.
Technical solution of the present invention is summarized as follows:
A kind of preparation method of pig farm microbial deodorant, includes the following steps,
(1) stalk is ground into stalk powder of the partial size within the scope of 0.5~1.0cm；Anoxybiotic is forged at 600~700 DEG C
1.5~2.5h is burnt, Temperature fall to room temperature obtains charcoal；
(2) bacillus after activation is inoculated into bacillus liquid culture medium, cultivates, obtains content >
109The bacillus bacterium solution of CFU/mL；By the lactobacillus inoculum after activation into lactic acid bacteria fluid nutrient medium, culture is contained
Measure > 109The lactic acid bacterial liquid of CFU/mL；Saccharomycete after activation is inoculated into saccharomycete fluid nutrient medium, cultivates, obtains
Content > 109The saccharomycete bacterium solution of CFU/mL；Bacillus bacterium solution, lactic acid bacterial liquid and saccharomycete bacterium solution are mixed in equal volume,
Obtain composite bacteria liquid；
(3) in mass ratio be 1:1~1.5 ratio, charcoal is uniformly mixed with the composite bacteria liquid, at 45~55 DEG C
Under constant temperature, stirring is cultivated 60~80 hours；
(4) it is separated by solid-liquid separation, solid drying obtains a kind of pig farm microbial deodorant.
Stalk is at least one of corn stover and broomcorn straw, can also select other stalks.
Bacillus is at least one of bacillus megaterium and bacillus subtilis.
Lactic acid bacteria is lactobacillus acidophilus.
Saccharomycete is that class schemes at least one of British mold and usual candidiasis.
Bacillus liquid culture substrate amount proportion are as follows: 2.0% cornstarch, 1.0% corn pulp, 0.5% sulphur
Sour ammonium, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 0.1% sodium chloride, remaining is water；pH6.5.
Lactic acid bacteria liquid culture substrate amount proportion are as follows: 1.0% peptone, 1.0% beef extract, 0.2% citric acid
Hydrogen diammonium, 2.0% glucose, 0.1% Tween 80,0.5% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.05% sulphur
Sour magnesium, 0.025% manganese sulfate, remaining is water；pH6.5.
Saccharomycete liquid culture substrate amount proportion are as follows: 8.0% molasses, 1.0% corn pulp, 0.5% ammonium sulfate,
0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 0.1% sodium chloride, remaining is water；pH6.5.
The pig farm microbial deodorant of above method preparation.
A kind of pig farm microbial deodorant of the invention, energy secretion activity substance promote beneficial microbe growth, generate
Fragrance；So that organic matter is fermented, adjust pH, inhibits harmful bacteria growth；Enzyme is generated, the generation of foul gas is inhibited.Ring of the present invention
Protect that efficient, at low cost, preparation method is easy, environmental protection.
Specific embodiment
Bacillus megaterium, bacillus subtilis, lactobacillus acidophilus, class figure British mold and usual candida strain
It is commercially available.
The present invention is further illustrated combined with specific embodiments below.
Saccharomycete slant medium quality proportioning are as follows: 4.0% brown sugar, 1.0% corn pulp, 0.5% peptone,
1.0% sodium chloride, remaining is water.
Saccharomycete liquid culture substrate amount proportion are as follows: 8.0% molasses, 1.0% corn pulp, 0.5% ammonium sulfate,
0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 0.1% sodium chloride, remaining is water；pH6.5；
Lactic acid bacteria slant medium quality proportioning are as follows: 0.5% peptone, 2.0% corn pulp, 4.0% sucrose,
0.5% sodium acetate, 0.5% magnesium sulfate, 1.0% sodium chloride, remaining is water.
Lactic acid bacteria liquid culture substrate amount proportion are as follows: 1.0% peptone, 1.0% beef extract, 0.2% citric acid
Hydrogen diammonium, 2.0% glucose, 0.1% Tween 80,0.5% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.05% sulphur
Sour magnesium, 0.025% manganese sulfate, remaining is water；pH6.5.
Bacillus slant medium quality proportioning are as follows: 0.5% peptone, 1.0% corn pulp, 1.0% chlorination
Sodium, remaining is water；
Bacillus liquid culture substrate amount proportion are as follows: 2.0% cornstarch, 1.0% corn pulp, 0.5% sulphur
Sour ammonium, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 0.1% sodium chloride, remaining is water；pH6.5.
Class figure British mold is inoculated in saccharomycete slant medium, after activating 20h under the conditions of 35 DEG C, is activated
Class afterwards schemes British mold.
Candida inconspicua bacterium is inoculated in saccharomycete slant medium, after activating 20h under the conditions of 35 DEG C, is activated
Candida inconspicua bacterium afterwards.
Lactobacillus acidophilus is inoculated in lactic acid bacteria slant medium, after activating 18h under the conditions of 35 DEG C, after being activated
Lactobacillus acidophilus.
Bacillus megaterium is inoculated in bacillus slant medium, after activating 18h under the conditions of 35 DEG C, is activated
Bacillus megaterium afterwards.
Bacillus subtilis is inoculated in bacillus slant medium, after activating 18h under the conditions of 35 DEG C, is activated
Bacillus subtilis afterwards.
Embodiment 1
A kind of preparation method of pig farm microbial deodorant, includes the following steps,
(1) the stalk powder by corn straw smashing at partial size within the scope of 0.5~1.0cm；Anoxybiotic is calcined at 650 DEG C
2h, Temperature fall to room temperature, obtains charcoal；
(2) by after activation bacillus megaterium and bacillus subtilis be inoculated into bacillus liquid culture medium respectively
In, culture obtains content > 10 respectively9The bacillus megaterium bacterium solution and content > 10 of CFU/mL9The bacillus subtilis of CFU/mL
Bacterium solution；Lactobacillus acidophilus after activation is inoculated into lactic acid bacteria fluid nutrient medium, cultivates, obtains content > 109CFU/mL's
Lactobacillus acidophilus bacterium solution；Class after activation is schemed into British mold and usual candidiasis is inoculated into the training of saccharomycete liquid respectively
It supports in base, culture obtains content > 10 respectively9The class's figure British mold bacterium solution and content > 10 of CFU/mL9The usual vacation of CFU/mL
Silk saccharomycete bacterium solution；It is by volume the ratio of 15:15:30:15:15 by bacillus megaterium bacterium solution, bacillus subtilis bacterium
Liquid, lactobacillus acidophilus bacterium solution, class's figure British mold bacterium solution and the mixing of usual candidiasis bacterium solution, obtain composite bacteria liquid, (multiple
Bacteria concentration is 1.1 × 10 in combined bacteria liquid9CFU/ml)；
(3) it is in mass ratio the ratio of 1:1, charcoal is uniformly mixed with the composite bacteria liquid, in 50 DEG C of constant temperatures
Under, stirring is cultivated 70 hours；
(4) it is separated by solid-liquid separation, solid drying obtains a kind of pig farm microbial deodorant.
Embodiment 2
A kind of preparation method of pig farm microbial deodorant, includes the following steps,
(1) the stalk powder by corn straw smashing at partial size within the scope of 0.5~1.0cm；Anoxybiotic is calcined at 600 DEG C
2.5h, Temperature fall to room temperature, obtains charcoal；
(2) by after activation bacillus megaterium and bacillus subtilis be inoculated into bacillus liquid culture medium respectively
In, culture obtains content > 10 respectively9The bacillus megaterium bacterium solution and content > 10 of CFU/mL9The bacillus subtilis of CFU/mL
Bacterium solution；Lactobacillus acidophilus after activation is inoculated into lactic acid bacteria fluid nutrient medium, cultivates, obtains content > 109CFU/mL's
Lactobacillus acidophilus bacterium solution；Candida inconspicua bacterium after activation is inoculated into saccharomycete fluid nutrient medium, cultivates, obtains content >
109The candida inconspicua bacterium bacterium solution of CFU/mL；By volume for 15:15:30:30 ratio by bacillus megaterium bacterium solution,
Bacillus subtilis bacterium solution, lactobacillus acidophilus bacterium solution and the mixing of usual candidiasis bacterium solution, obtain composite bacteria liquid (composite bacteria liquid
Middle bacteria concentration is 2 × 109CFU/ml)；
(3) it is in mass ratio the ratio of 1:1.2, charcoal is uniformly mixed with the composite bacteria liquid, in 55 DEG C of constant temperature items
Under part, stirring is cultivated 60 hours；
(4) it is separated by solid-liquid separation, solid drying obtains a kind of pig farm microbial deodorant.
Embodiment 3
A kind of preparation method of pig farm microbial deodorant, includes the following steps,
(1) corn stover and broomcorn straw that mass ratio is 1:1 are ground into straw of the partial size within the scope of 0.5~1.0cm
Stalk powder；Anoxybiotic is calcined 1.5h at 700 DEG C, and Temperature fall to room temperature obtains charcoal；
(2) bacillus subtilis after activation is inoculated into bacillus liquid culture medium, cultivates, obtains content >
109The bacillus subtilis bacterium solution of CFU/mL；Lactobacillus acidophilus after activation is inoculated into lactic acid bacteria fluid nutrient medium, is trained
It supports, obtains content > 109The lactobacillus acidophilus bacterium solution of CFU/mL；Class after activation is schemed into British mold and candida inconspicua
Bacterium is inoculated into respectively in saccharomycete fluid nutrient medium, and culture obtains content > 10 respectively9The class of CFU/mL schemes British mold bacterium solution
With content > 109The candida inconspicua bacterium bacterium solution of CFU/mL；It is by volume the ratio of 30:30:15:15 by bacillus subtilis
Bacterium bacterium solution, lactobacillus acidophilus bacterium solution, class's figure British mold bacterium solution and the mixing of usual candidiasis bacterium solution, obtain composite bacteria liquid
(bacteria concentration is 5 × 10 in composite bacteria liquid9CFU/ml)；
(3) it is in mass ratio the ratio of 1:1.5, charcoal is uniformly mixed with the composite bacteria liquid, in 45 DEG C of constant temperature items
Under part, stirring is cultivated 80 hours；
(4) it is separated by solid-liquid separation, solid drying obtains a kind of pig farm microbial deodorant.
Embodiment 4
The use of pig farm microbial deodorant:
A kind of pig farm microbial deodorant is put into swine excrement, the saccharomycete energy secretion activity in microbial bacterial agent
Substance promotes beneficial microbe growth, generates fragrance；Lactic acid bacteria can be carried out organic matter fermentation, adjust pH, inhibit harmful bacteria raw
It is long；Bacillus can generate enzyme, inhibit the release of foul gas.
The effect detection of microbial deodorant
Each 1.5 ㎏ of deodorizing microorganism that embodiment 1, embodiment 2 and embodiment 3 obtain is uniformly applied to pig manure 10m3In,
After effect 2 hours, ammonia, concentration of hydrogen sulfide are detected, testing result is as shown in table 1:
1 testing result of table
Note: (unit of ammonia and hydrogen sulfide is mg/m3)
As shown in Table 1, after pig farm microbial deodorant effect 2h, ammonia and concentration of hydrogen sulfide are than drop obvious before sprinkling
It is low.
Claims (9)
1. a kind of preparation method of pig farm microbial deodorant, it is characterised in that include the following steps,
(1) stalk is ground into stalk powder of the partial size within the scope of 0.5~1.0cm；The anoxybiotic calcination 1.5 at 600~700 DEG C
~2.5h, Temperature fall to room temperature, obtains charcoal；
(2) bacillus after activation is inoculated into bacillus liquid culture medium, cultivates, obtains content > 109CFU/mL's
Bacillus bacterium solution；By the lactobacillus inoculum after activation into lactic acid bacteria fluid nutrient medium, culture obtains content > 109CFU/
The lactic acid bacterial liquid of mL；Saccharomycete after activation is inoculated into saccharomycete fluid nutrient medium, cultivates, obtains content >
109The saccharomycete bacterium solution of CFU/mL；Bacillus bacterium solution, lactic acid bacterial liquid and saccharomycete bacterium solution are mixed in equal volume, answered
Combined bacteria liquid；
(3) in mass ratio be 1:1~1.5 ratio, charcoal is uniformly mixed with the composite bacteria liquid, in 45~55 DEG C of constant temperature
Under the conditions of, stirring is cultivated 60~80 hours；
(4) it is separated by solid-liquid separation, solid drying obtains a kind of pig farm microbial deodorant.
2. according to the method described in claim 1, it is characterized in that the stalk is at least one of corn stover and broomcorn straw.
3. according to the method described in claim 1, it is characterized in that the bacillus is bacillus megaterium and bacillus subtilis
At least one of bacterium.
4. according to the method described in claim 1, it is characterized in that the lactic acid bacteria is lactobacillus acidophilus.
5. according to the method described in claim 1, it is characterized in that the saccharomycete is that class schemes British mold and usually false silk ferment
At least one of female bacterium.
6. according to the method described in claim 1, it is characterized in that the bacillus liquid culture substrate amount matches are as follows: 2.0%
Cornstarch, 1.0% corn pulp, 0.5% ammonium sulfate, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 0.1%
Sodium chloride, remaining is water；pH6.5.
7. according to the method described in claim 1, it is characterized in that the lactic acid bacteria liquid culture substrate amount matches are as follows: 1.0%
Peptone, 1.0% beef extract, 0.2% diammonium hydrogen citrate, 2.0% glucose, 0.1% Tween 80,0.5%
Sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.025% manganese sulfate, remaining is water；pH6.5.
8. according to the method described in claim 1, it is characterized in that the saccharomycete liquid culture substrate amount matches are as follows: 8.0%
Molasses, 1.0% corn pulp, 0.5% ammonium sulfate, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 0.1% chlorination
Sodium, remaining is water；pH6.5.
9. pig farm microbial deodorant prepared by the method for one of claim 1-8.
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Cited By (5)
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| CN109987810A (en) * | 2019-04-22 | 2019-07-09 | 刘坚 | A kind of efficient pig manure fermentation method |
| CN110172418A (en) * | 2019-05-10 | 2019-08-27 | 浙江九田环保科技有限公司 | A kind of dedicated Bacteria Culture particular solution formula of deodorization and preparation method thereof |
| CN110201661A (en) * | 2019-04-28 | 2019-09-06 | 中山大学 | A kind of manganese base charcoal of porous array structure and its preparation method and application |
| CN110813075A (en) * | 2019-08-27 | 2020-02-21 | 上海普乐拜格健康科技有限公司 | Biological agent for eliminating peculiar smell of wet garbage and preparation method thereof |
| CN111686287A (en) * | 2020-06-30 | 2020-09-22 | 北京星皓互动生物科技有限公司 | Preparation method and application of microbial bacteriostatic deodorant |
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