JPH0310393B2 - - Google Patents
Info
- Publication number
- JPH0310393B2 JPH0310393B2 JP7777488A JP7777488A JPH0310393B2 JP H0310393 B2 JPH0310393 B2 JP H0310393B2 JP 7777488 A JP7777488 A JP 7777488A JP 7777488 A JP7777488 A JP 7777488A JP H0310393 B2 JPH0310393 B2 JP H0310393B2
- Authority
- JP
- Japan
- Prior art keywords
- sulfate
- bacteria
- reducing bacteria
- methane
- growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 90
- 241000894006 Bacteria Species 0.000 claims description 68
- 239000000126 substance Substances 0.000 claims description 29
- 238000000855 fermentation Methods 0.000 claims description 23
- 239000002351 wastewater Substances 0.000 claims description 23
- 238000011282 treatment Methods 0.000 claims description 17
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 16
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 16
- DOQPXTMNIUCOSY-UHFFFAOYSA-N [4-cyano-4-(3,4-dimethoxyphenyl)-5-methylhexyl]-[2-(3,4-dimethoxyphenyl)ethyl]-methylazanium;chloride Chemical compound [H+].[Cl-].C1=C(OC)C(OC)=CC=C1CCN(C)CCCC(C#N)(C(C)C)C1=CC=C(OC)C(OC)=C1 DOQPXTMNIUCOSY-UHFFFAOYSA-N 0.000 claims description 13
- 230000002401 inhibitory effect Effects 0.000 claims description 13
- 229960000881 verapamil hydrochloride Drugs 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 11
- 238000006722 reduction reaction Methods 0.000 claims description 11
- -1 hibitene Chemical compound 0.000 claims description 7
- RLSSMJSEOOYNOY-UHFFFAOYSA-N m-cresol Chemical compound CC1=CC=CC(O)=C1 RLSSMJSEOOYNOY-UHFFFAOYSA-N 0.000 claims description 6
- QWVGKYWNOKOFNN-UHFFFAOYSA-N o-cresol Chemical compound CC1=CC=CC=C1O QWVGKYWNOKOFNN-UHFFFAOYSA-N 0.000 claims description 4
- AKUVRZKNLXYTJX-UHFFFAOYSA-N 3-benzylazetidine Chemical compound C=1C=CC=CC=1CC1CNC1 AKUVRZKNLXYTJX-UHFFFAOYSA-N 0.000 claims description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 3
- 229960001657 chlorpromazine hydrochloride Drugs 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 claims description 3
- KVCGISUBCHHTDD-UHFFFAOYSA-M sodium;4-methylbenzenesulfonate Chemical compound [Na+].CC1=CC=C(S([O-])(=O)=O)C=C1 KVCGISUBCHHTDD-UHFFFAOYSA-M 0.000 claims description 3
- 229950002929 trinitrophenol Drugs 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 description 17
- 238000012360 testing method Methods 0.000 description 15
- 239000007789 gas Substances 0.000 description 14
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000001257 hydrogen Substances 0.000 description 5
- 229910052739 hydrogen Inorganic materials 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000186538 Desulfotomaculum nigrificans Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-YPZZEJLDSA-N carbon-10 atom Chemical compound [10C] OKTJSMMVPCPJKN-YPZZEJLDSA-N 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- QTWJRLJHJPIABL-UHFFFAOYSA-N 2-methylphenol;3-methylphenol;4-methylphenol Chemical compound CC1=CC=C(O)C=C1.CC1=CC=CC(O)=C1.CC1=CC=CC=C1O QTWJRLJHJPIABL-UHFFFAOYSA-N 0.000 description 1
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical compound [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 description 1
- 241000205286 Methanosarcina sp. Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 229930003836 cresol Natural products 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- IMBKASBLAKCLEM-UHFFFAOYSA-L ferrous ammonium sulfate (anhydrous) Chemical compound [NH4+].[NH4+].[Fe+2].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O IMBKASBLAKCLEM-UHFFFAOYSA-L 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 238000011221 initial treatment Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 125000001434 methanylylidene group Chemical group [H]C#[*] 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Treatment Of Sludge (AREA)
Description
「産業上の利用分野」
この発明は、メタン発酵等の嫌気性発酵によつ
て有機性廃水を処理する場合に行なわれる嫌気性
処理における硫酸還元反応抑制方法および硫酸還
元菌阻害性物質に関するものである。
「従来の技術」
メタン発酵などの嫌気性発酵による有機性廃水
の処理は、活性汚泥法等の好気性処理に比べて、
曝気動力が不用、汚泥生成量が少ない、エ
ネルギー源として利用できるメチンガスが生成す
る、高負荷の一次処理ができる、等の利点を持
つ水処理法であるが、この方法は特に有機物を高
濃度に含む廃水の処理に適している。
「発明が解決しようとする課題」
しかしながら、上記のような高濃度の有機性廃
水のなかには、アルコール蒸留廃液、バルブ廃液
等のように、しばしば多量の硫酸イオンを含むも
のがある。このような廃水をメタン発酵させる
と、メタン細菌と同様の生育条件下で硫酸還元菌
が繁殖し、硫酸イオンがメタン細菌の生育および
メタン発酵を阻害する硫化物イオンに還元される
ため、メタン発酵が抑制されてメタン生成量、廃
水処理能力が低下し、著しい場合には、メタン発
酵が停止する。また、本来、メタン生成に使われ
る水素が硫酸イオンの還元に使われるため、生成
ガス中のメタン濃度が低下し、生成ガスのほとん
どが炭酸ガスとなつてエネルギー源としての価値
が低下する。さらに、生成ガス中に腐食性の硫化
水素ガスの濃度が増加する。
このため、嫌気性処理を行なう場合には、廃水
中の硫酸還元菌の生育を阻害することによつて硫
酸イオンの還元を抑制することが必要と考えられ
ており、そのために上記廃水中に硫酸還元菌を阻
害する物質を添加することも考えられるが、従来
は、そのような物質がメタン細菌に及ぼす影響が
知られていなかつたため、嫌気性処理において廃
水中に硫酸還元菌を阻害する物質を添加する試み
は未だ行なわれたことがなかつた。
この発明は、上記事情に鑑みてなされたもの
で、硫酸還元菌の生育を阻害すると共にメタン細
菌の生育を阻害しない物質を供し、かつ、その物
質により廃水中の硫酸還元反応を抑制して嫌気性
処理を良好な状態で行なうことを目的としてい
る。
「課題を解決するための手段」
この発明の嫌気性処理における硫酸還元反応抑
制方法は、硫酸イオンを含む有機性廃水を嫌気性
発酵によつて処理する際に、上記有機性廃水にメ
タン細菌と共存する硫酸還元菌の生育を選択的に
阻害する物質を添加するものである。
また、この発明の硫酸還元菌阻害性物質は、ベ
ラパミル塩酸塩、クロルプロマジン塩酸塩、ｏ−
クレゾール、ｍ−クレゾール、ヒビテン、トリニ
トロフエノール、ｐ−トルエンスルホン酸ナトリ
ウム、グルタルアルデヒドから選ばれたうちの一
種類または二種類以上の混合物からなるものであ
る。
「作用」
この発明の嫌気性処理における硫酸還元反応抑
制方法においては、硫酸イオンを含む有機性廃水
を嫌気性発酵によつて処理する際に、上記有機性
廃水にメタン細菌と共存する硫酸還元菌の生育を
選択的に阻害する物質を添加することによつて、
上記硫酸還元菌による硫酸イオンの還元を抑制し
てメタン発酵を阻害する酸化物イオンの発生を防
ぐと共に上記硫酸還元菌がメタン生成に必要な水
素を消費することも防止する。このため、この方
法を用いて有機性廃水の嫌気性処理を行なうと、
メタン発酵が良好な状態で行なわれて、廃水処理
が効果的に行なわれることとなる。
また、この発明の硫酸還元菌阻害性物質を用い
た場合には、有機性廃水の嫌気性処理を効率的に
行なうことができる上、その硫酸還元菌阻害性に
よつて、鉄製のタンク、配管等が硫酸還元菌がつ
くりだす硫化水素ガスで腐食されることも防止す
ることができ、硫酸還元菌による被害に対して広
く応用することが可能である。
「実施例」
以下、この発明の一実施例を説明する。
この実施例では、例えばアルコール蒸留廃液あ
るいはパルプ廃液等のように硫酸イオンを含む有
機性廃水中に、硫酸還元菌の生育を阻害すると共
にメタン細菌の生育を阻害しない硫酸還元菌阻害
性物質を添加し、その有機性廃水をメタン発酵さ
せて嫌気性処理を行なう。
このような硫酸還元菌阻害性物質としては、例
えばベラパミル塩酸塩などがある。そして、この
ベラパミル塩酸塩を例えば10kt程度の適当な濃度
になるようにして発酵槽（あるいはリアクター、
消火槽等）に添加すると、ベラパミル塩酸塩の作
用により硫酸還元菌の活動が抑制されるため、硫
酸イオンの硫化物イオンへの還元が起らず、メタ
ン発酵を良好な状態で行なうことができる。
また、上記ベラパミル塩酸塩のような硫酸還元
菌阻害性物質は、硫酸還元菌による腐食性の硫化
水素ガスの発生も抑制できるため、反応処理にお
ける発酵槽、リアクター、または消火槽等ばかり
でなく、土中等の嫌気下における鉄製の送油管、
ガス管、および水道管等が硫化水素ガスで腐食さ
れることも防止することができる。
なお、上記硫酸還元菌阻害性物質としては、上
記ベラパミル塩酸塩の他、クロルプロマジン塩酸
塩、ｏ−クレゾール、ｍ−クレゾール、ヒビテ
ン、トリニトロフエノール、ｐ−トルエンスルホ
ン酸ナトリウム、グルタルアルデヒドおよびその
他の物質などがある。そして、これらの物質を用
いた場合にも、ベラパミル塩酸塩を用いた場合と
同様な効果を奏することができる。
「実施例」
（実験１） スクリーニングテスト
硫酸還元菌の生育を阻害し、メタン細菌の生育
を阻害しない物質を捜すため、表１に示すフロー
に基づいてスリーニングテストを実施した。
このスクリーニングテストにおいては、メタン
細菌として、メタノールを炭素源として利用可能
で、しかもそのメタン発酵域の至適生育温度が55
℃のメタノサルシナ（Methanosarcina sp.
（DSM2906））を用い、硫酸還元菌として、上記
至適生育温度とほぼ等しい至適生育温度を持つデ
スルホトマクルム ニグリフインカス
（Desulufotomaculumnigrificans（DSM574））を
用いた。そして、これら各菌の菌株に対して、表
２、表３に示す培地を調整した。
"Industrial Application Field" The present invention relates to a method for inhibiting sulfate reduction reaction in anaerobic treatment when organic wastewater is treated by anaerobic fermentation such as methane fermentation, and a substance that inhibits sulfate-reducing bacteria. be. "Conventional technology" Treatment of organic wastewater by anaerobic fermentation such as methane fermentation is more effective than aerobic treatment such as activated sludge method.
This water treatment method has the following advantages: it does not require aeration power, produces less sludge, produces methine gas that can be used as an energy source, and can perform high-load primary treatment. Suitable for treating wastewater containing ``Problems to be Solved by the Invention'' However, among the above-mentioned highly concentrated organic wastewaters, some, such as alcohol distillation wastewater and valve wastewater, often contain large amounts of sulfate ions. When such wastewater is subjected to methane fermentation, sulfate-reducing bacteria proliferate under the same growth conditions as methane bacteria, and sulfate ions are reduced to sulfide ions that inhibit the growth of methane bacteria and methane fermentation, resulting in methane fermentation. is suppressed, resulting in a decrease in methane production and wastewater treatment capacity, and in severe cases, methane fermentation stops. Additionally, since the hydrogen normally used to generate methane is used to reduce sulfate ions, the methane concentration in the produced gas decreases, and most of the produced gas becomes carbon dioxide, reducing its value as an energy source. Additionally, the concentration of corrosive hydrogen sulfide gas in the product gas increases. Therefore, when performing anaerobic treatment, it is considered necessary to suppress the reduction of sulfate ions by inhibiting the growth of sulfate-reducing bacteria in the wastewater. It is possible to add substances that inhibit sulfate-reducing bacteria, but until now, the effect of such substances on methane bacteria was not known, so it was not possible to add substances that inhibit sulfate-reducing bacteria to wastewater during anaerobic treatment. Attempts to add it have not yet been made. This invention was made in view of the above circumstances, and provides a substance that inhibits the growth of sulfate-reducing bacteria and does not inhibit the growth of methane bacteria, and also suppresses the sulfuric acid reduction reaction in wastewater, thereby making it anaerobic. The purpose is to perform sexual processing in good condition. "Means for Solving the Problems" The method for suppressing sulfuric acid reduction reaction in anaerobic treatment of the present invention is a method for suppressing sulfuric acid reduction reaction in anaerobic treatment, when organic wastewater containing sulfate ions is treated by anaerobic fermentation, methane bacteria are added to the organic wastewater. A substance is added that selectively inhibits the growth of coexisting sulfate-reducing bacteria. In addition, the sulfate-reducing bacteria-inhibiting substances of this invention include verapamil hydrochloride, chlorpromazine hydrochloride, o-
It consists of one type or a mixture of two or more types selected from cresol, m-cresol, hibitene, trinitrophenol, sodium p-toluenesulfonate, and glutaraldehyde. "Function" In the method for suppressing sulfuric acid reduction reaction in anaerobic treatment of the present invention, when organic wastewater containing sulfate ions is treated by anaerobic fermentation, sulfuric acid reducing bacteria coexisting with methane bacteria are added to the organic wastewater. By adding a substance that selectively inhibits the growth of
The reduction of sulfate ions by the sulfate-reducing bacteria is suppressed to prevent the generation of oxide ions that inhibit methane fermentation, and the sulfate-reducing bacteria are also prevented from consuming hydrogen necessary for methane production. Therefore, when using this method for anaerobic treatment of organic wastewater,
Methane fermentation is carried out in good condition, and wastewater treatment is carried out effectively. Furthermore, when the sulfate-reducing bacteria-inhibiting substance of the present invention is used, it is possible to efficiently perform anaerobic treatment of organic wastewater, and due to its sulfate-reducing bacteria-inhibiting property, iron tanks, piping, etc. It can also prevent the corrosion of hydrogen sulfide gas produced by sulfate-reducing bacteria, and can be widely applied to damage caused by sulfate-reducing bacteria. “Example” An example of the present invention will be described below. In this example, a sulfate-reducing bacteria-inhibiting substance that inhibits the growth of sulfate-reducing bacteria and does not inhibit the growth of methane bacteria is added to organic wastewater containing sulfate ions, such as alcohol distillation waste liquid or pulp waste liquid. The organic wastewater is then subjected to methane fermentation for anaerobic treatment. Examples of such sulfate-reducing bacteria-inhibiting substances include verapamil hydrochloride. Then, this verapamil hydrochloride is brought to an appropriate concentration of, for example, about 10 kt, and then placed in a fermenter (or reactor).
When added to fire extinguishing tanks, etc., the activity of sulfate-reducing bacteria is suppressed by the action of verapamil hydrochloride, so sulfate ions are not reduced to sulfide ions, and methane fermentation can be carried out in good conditions. . In addition, sulfate-reducing bacteria-inhibiting substances such as verapamil hydrochloride can also suppress the generation of corrosive hydrogen sulfide gas by sulfate-reducing bacteria, so they can be used not only in fermenters, reactors, fire tanks, etc. in reaction processing, but also in Steel oil pipes under anaerobic conditions such as soil,
It is also possible to prevent gas pipes, water pipes, etc. from being corroded by hydrogen sulfide gas. In addition to the above-mentioned verapamil hydrochloride, the above-mentioned sulfate-reducing bacteria-inhibiting substances include chlorpromazine hydrochloride, o-cresol, m-cresol, hibitene, trinitrophenol, sodium p-toluenesulfonate, glutaraldehyde, and other substances. and so on. Even when these substances are used, the same effects as when verapamil hydrochloride is used can be achieved. "Example" (Experiment 1) Screening Test In order to search for a substance that inhibits the growth of sulfate-reducing bacteria and does not inhibit the growth of methane bacteria, a screening test was conducted based on the flow shown in Table 1. In this screening test, methane bacteria were able to utilize methanol as a carbon source, and the optimal growth temperature for their methane fermentation range was 55°C.
Methanosarcina sp.
(DSM2906)) was used as the sulfate-reducing bacterium, and Desulfotomaculum nigrificans (DSM574), which has an optimal growth temperature almost equal to the above-mentioned optimal growth temperature, was used. Then, the culture media shown in Tables 2 and 3 were prepared for each of these bacterial strains.
【表】【table】
【表】
但し、上記培地はオートクレープにより、120
℃で15分間殺菌処理され、そのPHは7.2に調整さ
れている。そして、このスクリーニングテスト
は、窒素80％、二酸化炭素10％、水素10％の雰囲
気中において行なつた。[Table] However, the above culture medium was prepared by autoclaving at 120%
It is sterilized at ℃ for 15 minutes and its pH is adjusted to 7.2. This screening test was conducted in an atmosphere of 80% nitrogen, 10% carbon dioxide, and 10% hydrogen.
【表】
但し、上記培地はオートクレープにより120℃
で15分間殺菌処理され、また、そのPHは7.2に調
整されている。そして、このスクリーニングテス
トは、窒素80％、二酸化炭素10％、水素10％の雰
囲気中において行なつた。
なお、表３中におけるビタミン溶液および微量
元素溶液の配合をそれぞれ表４、表５に示す。[Table] However, the above medium is heated to 120℃ by autoclaving.
It is sterilized for 15 minutes and its pH is adjusted to 7.2. This screening test was conducted in an atmosphere of 80% nitrogen, 10% carbon dioxide, and 10% hydrogen. The formulations of the vitamin solution and trace element solution in Table 3 are shown in Table 4 and Table 5, respectively.
【表】【table】
【表】【table】
【表】
このようなスクリーニングテストにおいて、培
地に硫酸還元菌が生存、生育する場合には、硫酸
塩が乳酸ナトリウムを還元剤として還元され、モ
ール塩中の鉄と作用して硫化鉄を生成し、培地が
黒変する。このため、このスクリーニングテスト
では、培地の黒変によつて硫酸還元菌の生育確認
とした。また、メタン細菌は、生育すると、培養
液の入つた試験管の下部に根粒状となつて沈澱す
るため、培養液は濁らない。さらに、メタン細菌
の生育に伴つてメタンガスが発生するので、これ
をもつて生育確認とする。
そして、このようにして多数の物質についてス
クリーニングテストを行つた結果、表６に示す８
種の物質が有効と認められた。
なお、各物質名の右側に記載した有効濃度と
は、メタン発酵が最も良好な状態で行なわれる濃
度であるが、これ以外の濃度でも硫酸還元菌の生
育を抑制することが可能であり、その場合にもメ
タン発酵を効率的に行なうことができる。[Table] In such a screening test, if sulfate-reducing bacteria survive and grow in the culture medium, sulfate is reduced using sodium lactate as a reducing agent and interacts with iron in Mohr's salt to produce iron sulfide. , the medium turns black. Therefore, in this screening test, the growth of sulfate-reducing bacteria was confirmed by the blackening of the medium. Furthermore, when methane bacteria grow, they precipitate in the form of nodules at the bottom of the test tube containing the culture solution, so the culture solution does not become cloudy. Furthermore, as methane bacteria grow, methane gas is generated, and this is used to confirm growth. As a result of conducting screening tests on a large number of substances in this way, we found that the 8 substances shown in Table 6
Seed substances were recognized as effective. The effective concentration listed to the right of each substance name is the concentration at which methane fermentation occurs in the best conditions, but it is also possible to suppress the growth of sulfate-reducing bacteria at other concentrations. In some cases, methane fermentation can be carried out efficiently.
【表】【table】
【表】
（実験２） 共生テスト
実験１で有効と認められた表６の物質のうちか
らベラパミル塩酸塩を選び、表７の培地を用いて
共生テストを行つた。
この共生テストでは、実験１と同様な実験方法
で行なわれ、メタン細菌の単独培養、メタン細菌
と硫酸還元菌との混合培養、および上記ベラパミ
ル塩酸塩を10ppm添加したメタン細菌と硫酸還元菌
との混合培養を行なつた。そして、メタン細菌の
増殖は、メタンガス発生量をモニターすることに
より検知し、硫酸還元菌の増殖は、硫酸イオン濃
度の変化をモニターすることにより検知した。[Table] (Experiment 2) Symbiotic test Verapamil hydrochloride was selected from the substances shown in Table 6 that were found to be effective in Experiment 1, and a symbiotic test was conducted using the medium shown in Table 7. This symbiosis test was conducted using the same experimental methods as Experiment 1, including single culture of methane bacteria, mixed culture of methane bacteria and sulfate-reducing bacteria, and cultivation of methane bacteria and sulfate-reducing bacteria to which 10 ppm of verapamil hydrochloride was added. A mixed culture was performed. Growth of methane bacteria was detected by monitoring the amount of methane gas generated, and growth of sulfate-reducing bacteria was detected by monitoring changes in sulfate ion concentration.
【表】【table】
【表】
但し、上記培地は、PHが7.0に調整されている。
この共生テストの結果、第１図、第２図に示す
ように、メタン細菌と硫酸還元菌とを混合した系
にベラパミル塩酸塩を添加した場合には、メタン
細菌単独の系と同様に良好なメタン発酵が行なわ
れ、かつ硫酸還元菌の増殖も抑制された。
また、上記共生テストと同様に表７の培地を用
いて、上記表６中に記載した上記ベラパミル塩酸
塩以外の各物質を添加したメタン細菌と硫酸還元
菌との混合培養を行なつたところ、これら各物質
を添加した場合にも、上記ベラパミル塩酸塩を添
加した場合と同様に良好なメタン発酵が行なわ
れ、かつ硫酸還元菌の増殖も抑制された。
「発明の効果」
この発明の嫌気性処理における硫酸還元反応抑
制方法によれば、硫酸イオンを含む有機性廃水を
嫌気性発酵によつて処理する際に、上記有機性廃
水にメタン細菌と共存する硫酸還元菌の生育を選
択的に阻害する物質を添加するので、上記硫酸還
元菌による硫酸イオンの還元を抑制してメタン発
酵を阻害する硫化物イオンの発生を防ぐことがで
きると共に、メタン生成に必要な水素が上記硫酸
還元菌に消費されることを防止することができ
る。このため、この方法を用いて有機性廃水の嫌
気性処理を行なつた場合には、メタン発酵を良好
な状態で行なうことができ、廃水処理を効果的に
行なうことができる。
また、この発明の硫酸還元菌阻害性物質によれ
ば、硫酸還元菌の生育を阻害すると共にメタン細
菌の生育を阻害しないために嫌気性処理を効率的
に行なうことができるだけでなく、その硫酸還元
菌阻害性によつて、その嫌気性処理で用いられる
鉄製のタンク、配管などが硫酸還元菌をつくりだ
す硫化水素ガスで腐食されることも防止すること
ができる。そして、この硫酸還元菌阻害性物質に
れば、土中等の嫌気したにおける鉄製の送油管、
ガス管、水道管などが硫酸還元菌がつくりだす硫
化水素イオンで腐食されることも防止することが
でき、その他の硫酸還元菌による被害に対しても
広く応用して幅広い産業分野に利用することが可
能である。[Table] However, the pH of the above medium is adjusted to 7.0. As a result of this symbiosis test, as shown in Figures 1 and 2, when verapamil hydrochloride was added to a mixed system of methane bacteria and sulfate-reducing bacteria, it was found to be as good as a system with methane bacteria alone. Methane fermentation was carried out, and the growth of sulfate-reducing bacteria was also suppressed. In addition, as in the symbiosis test above, a mixed culture of methane bacteria and sulfate-reducing bacteria was carried out using the medium shown in Table 7 to which each substance other than the verapamil hydrochloride listed in Table 6 was added. When each of these substances was added, methane fermentation was carried out as well as in the case where verapamil hydrochloride was added, and the growth of sulfate-reducing bacteria was also suppressed. "Effects of the Invention" According to the method for suppressing sulfuric acid reduction reaction in anaerobic treatment of the present invention, when organic wastewater containing sulfate ions is treated by anaerobic fermentation, methane bacteria coexist in the organic wastewater. Since a substance that selectively inhibits the growth of sulfate-reducing bacteria is added, it is possible to suppress the reduction of sulfate ions by the sulfate-reducing bacteria and prevent the generation of sulfide ions that inhibit methane fermentation. It is possible to prevent necessary hydrogen from being consumed by the sulfate-reducing bacteria. Therefore, when organic wastewater is anaerobically treated using this method, methane fermentation can be carried out in good conditions, and wastewater treatment can be carried out effectively. Further, according to the sulfate-reducing bacteria-inhibiting substance of the present invention, it is possible to not only inhibit the growth of sulfate-reducing bacteria and not inhibit the growth of methane bacteria, so that anaerobic treatment can be carried out efficiently, but also to inhibit the sulfate-reducing bacteria. Due to its bacteria-inhibiting properties, it can also prevent iron tanks and piping used in the anaerobic treatment from being corroded by hydrogen sulfide gas, which produces sulfate-reducing bacteria. If this sulfate-reducing bacteria inhibitory substance is present, iron oil pipes in anaerobic environments such as soil, etc.
It can also prevent gas pipes, water pipes, etc. from being corroded by hydrogen sulfide ions produced by sulfate-reducing bacteria, and can be widely applied to prevent damage caused by other sulfate-reducing bacteria and used in a wide range of industrial fields. It is possible.
第１図、第２図は、共生テストの結果を示す図
であつて、第１図は培養時間と硫酸イオン濃度と
の関係を表すグラフ、第２図は培養時間とメタン
ガス発生量との関係を表すグラフである。
Figures 1 and 2 are diagrams showing the results of the symbiosis test, with Figure 1 being a graph showing the relationship between culture time and sulfate ion concentration, and Figure 2 being a graph showing the relationship between culture time and methane gas generation amount. This is a graph representing
Claims (1)
よつて処理する際に、上記有機性廃水にメタン細
菌と共存する硫酸還元菌の生育を選択的に阻害す
る物質を添加することを特徴とする嫌気性処理に
おける硫酸還元反応抑制方法。 ２ 第１項記載の嫌気性処理における硫酸還元反
応抑制方法で添加する物質において、ベラパミル
塩酸塩、クロルプロマジン塩酸塩、ｏ−クレゾー
ル、ｍ−クレゾール、ヒビテン、トリニトロフエ
ノール、ｐ−トルエンスルホン酸ナトリウム、グ
ルタルアルデヒドから選ばれたうちの一種類また
は二種類以上の混合物からなることを特徴とする
硫酸還元菌阻害性物質。[Claims] 1. When organic wastewater containing sulfate ions is treated by anaerobic fermentation, a substance that selectively inhibits the growth of sulfate-reducing bacteria that coexists with methane bacteria is added to the organic wastewater. A method for suppressing sulfuric acid reduction reaction in anaerobic treatment. 2. Among the substances added in the method for suppressing sulfuric acid reduction reaction in anaerobic treatment described in item 1, verapamil hydrochloride, chlorpromazine hydrochloride, o-cresol, m-cresol, hibitene, trinitrophenol, sodium p-toluenesulfonate, A sulfate-reducing bacteria-inhibiting substance characterized by comprising one type or a mixture of two or more types selected from glutaraldehyde.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63077774A JPH01249198A (en) | 1988-03-30 | 1988-03-30 | Method for inhibiting reduction of sulfate in anaerobic treatment and substance hindering growth of sulfate reducing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63077774A JPH01249198A (en) | 1988-03-30 | 1988-03-30 | Method for inhibiting reduction of sulfate in anaerobic treatment and substance hindering growth of sulfate reducing bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01249198A JPH01249198A (en) | 1989-10-04 |
| JPH0310393B2 true JPH0310393B2 (en) | 1991-02-13 |
Family
ID=13643302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63077774A Granted JPH01249198A (en) | 1988-03-30 | 1988-03-30 | Method for inhibiting reduction of sulfate in anaerobic treatment and substance hindering growth of sulfate reducing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01249198A (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01272502A (en) * | 1988-04-21 | 1989-10-31 | Shimizu Corp | Prevention against growing of sulfuric acid reduction fungi |
| JPH01270997A (en) * | 1988-04-21 | 1989-10-30 | Shimizu Corp | Process and apparatus for anaerobic fermentation |
| JPH01268603A (en) * | 1988-04-21 | 1989-10-26 | Shimizu Corp | Inhibition of growth of sulfuric acid-reducing bacterium |
| WO2013137322A1 (en) * | 2012-03-13 | 2013-09-19 | 株式会社ダイセル | Waste water treatment method |
| CN106854026B (en) * | 2016-10-28 | 2020-11-20 | 江苏迈达新材料股份有限公司 | Method for treating 2, 6-di-tert-butyl-p-methylphenol production waste liquid |
-
1988
- 1988-03-30 JP JP63077774A patent/JPH01249198A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01249198A (en) | 1989-10-04 |
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