JPH0239834A - Production of artificial mutant of colocasia antiquorum and breeding of colocasia antiquorum utilizing the same - Google Patents

Production of artificial mutant of colocasia antiquorum and breeding of colocasia antiquorum utilizing the same

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Publication number
JPH0239834A
JPH0239834A JP63189256A JP18925688A JPH0239834A JP H0239834 A JPH0239834 A JP H0239834A JP 63189256 A JP63189256 A JP 63189256A JP 18925688 A JP18925688 A JP 18925688A JP H0239834 A JPH0239834 A JP H0239834A
Authority
JP
Japan
Prior art keywords
taro
colocasia antiquorum
mutant
antiquorum
colocasia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP63189256A
Other languages
Japanese (ja)
Other versions
JPH0433416B2 (en
Inventor
Sadami Miyazaki
宮崎 貞巳
Hirosuke Tashiro
田代 洋丞
Masanobu Tanaka
政信 田中
Tatsuomi Tanaka
田中 龍臣
Joichi Urata
浦田 丈一
Takanori Matsuo
松尾 孝則
Hisaki Nakajima
中島 寿亀
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KATSUKI KUMAO
Original Assignee
KATSUKI KUMAO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KATSUKI KUMAO filed Critical KATSUKI KUMAO
Priority to JP63189256A priority Critical patent/JPH0239834A/en
Publication of JPH0239834A publication Critical patent/JPH0239834A/en
Publication of JPH0433416B2 publication Critical patent/JPH0433416B2/ja
Granted legal-status Critical Current

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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To effectively produce mutant of colocasia antiquorum by treating shoot apex of colocasia antiquorum to mutagenesis, separating said mutant and raising by aseptic culture. CONSTITUTION:Shoot apex of colocasia antiquorum is delivered, mutation is induced by treating said delivered apex with mutagen, resultant mutant is separated and raised by aseptic culture. Colocasia antiquorum to be said applying object is mainly colocasia antiquorum belonging to YATSUGASHIRA or EGUIMO group. YATSUGASHIRA group is well-known as the most tasty type among the types of colocasia antiquorum and a type of produced from old time. Besides, EGUIMO group is shipped to market after early maturing varieties such as ISHIKAWA WASE as late maturing variety and a type having strong cold resistance. Methyl nitrosourethane, sodium azide or ethyl methanesulfonic acid, etc., is used as mutagen.

Description

【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、高吸澱粉作物として賞味されているサトイモ
の調理の際に除皮し易い品種の作出法並びにその育種法
に関するものである。
DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for producing a variety of taro, which is enjoyed as a highly absorbent starch crop, and whose skin is easily removed during cooking, and a method for breeding the same.

〔従来の技術〕[Conventional technology]

サトイモは古くから食用として賞味され、現在でも世界
的に重要な澱粉作物である。特に「八つ頭」などの品種
は最も良食味品種として知られている。
Taro has been enjoyed as food since ancient times, and is still an important starch crop worldwide. In particular, varieties such as ``Yatsuzu'' are known as the best-tasting varieties.

しかしながら、これらの品種は球根の形状が調理に不便
であるため商品性が低く、一般に調理用として用い難い
点があった。
However, these varieties have low marketability because the shape of the bulbs is inconvenient for cooking, and are generally difficult to use for cooking.

そこで、調理し易い形状のサトイモ、特に「八つ頭」等
の品種の自然に生ずる突然変異を利用し、その突然変異
体を育種する方法が試みられ、さらに、その突然変異体
の出現頻度を高くするために、放射線(ガンマ−線)を
照射することが行われたが、成功するに至っていない。
Therefore, attempts have been made to utilize naturally occurring mutations in taro varieties that are easy to cook, especially varieties such as ``Yatsuzu'', and to breed the mutants. In order to increase the height, radiation (gamma rays) has been irradiated, but this has not been successful.

〔発明が解決しようとする課題〕[Problem to be solved by the invention]

上述の従来の技術においては、出現頻度が非常に低い自
然突然変異体を利用するため極めて効率が悪く、さらに
、放射線処理等の人為的突然変異処理を行った場合でも
、株の突然変異処理ではキメラになり易く、変異体をl
 N体として分離することが困難であった。
The above-mentioned conventional techniques are extremely inefficient because they use natural mutants with a very low frequency of occurrence, and furthermore, even when artificial mutation treatments such as radiation treatment are performed, mutation treatment of strains is not effective. prone to chimerism and mutants
It was difficult to separate it as N-isomer.

また、変異体が作出されたとしても、変異体が淘汰を受
は易く、自然の状態では死滅して変異体として分離でき
ない等の理由により、サトイモの調理し易い形状、除皮
し易い品種を効率良く得ることができなかった。
In addition, even if mutants are created, they are easily selected and die in nature, making it impossible to isolate them as mutants. could not be obtained efficiently.

本発明はサトイモの変異体を効率良く作出する方法並び
にその育種法を提供することを目的とするものである。
The purpose of the present invention is to provide a method for efficiently producing taro mutants and a breeding method thereof.

〔課題を解決するための手段〕[Means to solve the problem]

本発明者らは上述の目的を達成するために、人為的に突
然変異を誘起させて得ただサトイモの突然変異体を分離
し得る方法について鋭意研究の結果、摘出したサトイモ
の茎頂部を突然変異誘発処理し、これを無菌培養するこ
とにより突然変異細胞から個体を育て、培養中に突然変
異体を分離することによって、サトイモの変異体を効率
良く作出することを見出し本発明を完成した。
In order to achieve the above-mentioned purpose, the present inventors conducted intensive research on a method for isolating taro mutants obtained by artificially inducing mutations. We have completed the present invention by discovering that taro mutants can be efficiently produced by inducing cells, culturing them aseptically, growing individuals from mutant cells, and isolating the mutants during culture.

本発明はサトイモの茎頂部を摘出し、これを突然変異誘
起物質で処理することにより突然変異を誘発させた後、
無菌培養により突然変異体を分離、育成するサトイモの
人為突然変異体作出方法、並びに、この方法によって作
出されたサトイモの突然変異体を素材としてサトイモの
新植物を育種する方法である。これによって、調理の際
に除皮し易いサトイモを得ることができる。
The present invention involves removing the top of a taro stalk, treating it with a mutagenic substance to induce mutations, and then
These are a method for creating artificial taro mutants by isolating and growing mutants through sterile culture, and a method for breeding new taro plants using the taro mutants created by this method as materials. This makes it possible to obtain taro that is easy to remove the skin during cooking.

〔作用〕[Effect]

本発明の適用対象とするサトイモは、主として八つ頭群
、ニゲイモ群に属するサトイモである。
The taro to which the present invention is applied is mainly a taro belonging to the Yatozu group and the Nageimo group.

八つ頭群はサトイモ品種の中で最も良食味品種として知
られ、古くから作られている品種である。
Yatsuzu-gun is known as the best-tasting taro variety and has been produced for a long time.

また、ニゲイモ群は晩生種として品種「石川早生」等の
早生種群の後に市場に出荷され、耐寒性の強い品種であ
る。
In addition, the Nigeimo group is a late-maturing variety that is shipped to the market after the early-maturing variety such as the cultivar "Ishikawa Wase," and is a highly cold-resistant variety.

本発明においては、これらのサトイモの茎頂部を摘出し
、突然、変異誘発処理を行う。ここで、茎頂部は、生長
点とも呼ばれ、植物体先端の細胞***活性の高い組織で
ある。サトイモの場合は芽のなかにあり、円錐形をして
いる。この切り取った茎頂孔を殺菌して突然変異誘起物
質、例えばメチルニトロソウレアで処理するっ この処理方法は、切り取ったサトイモの茎頂部を、例え
ばメチルニトロソウレアの溶液の5〜10m M E後
の溶液に浸漬し、10〜40分前後処理する。
In the present invention, the stem tips of these taro are removed and suddenly subjected to mutagenesis treatment. Here, the shoot apex is also called a growing point, and is a tissue with high cell division activity at the tip of the plant body. In the case of taro, it is inside the bud and has a cone shape. This treatment method involves sterilizing the excised shoot apices and treating them with a mutagen, such as methylnitrosourea. Immerse in the solution and treat for 10 to 40 minutes.

この場合容器を振盪すると一層効果的である。In this case, shaking the container is more effective.

なお、突然変異誘起物質としては、メチルニトロソウレ
アのほかに、アジ化ナトリウム、エチルメタンスルホン
酸等があるが、本発明者等の実験によれば、メチルニト
ロソウレアが安定して高い頻度で突然変異を誘発するこ
とが確認された。
In addition to methylnitrosourea, mutagenic substances include sodium azide, ethylmethanesulfonic acid, etc., but according to experiments conducted by the present inventors, methylnitrosourea can be used stably and suddenly at a high frequency. It was confirmed that it induces mutations.

以上の処理が完了したサトイモ突然変異体をMS(Mu
rashige and Skoog)  培地、ホワ
イト(White)培地、 B5培地、  V’W(V
acin and Went)培地、狩野培地などの植
物組織培養に一般に利用される培地で無菌的に培養する
。これらの培地は第1表に示す組成を有しており、培養
する植物の種類により種々選択することができる。
The taro mutant that has undergone the above treatment is subjected to MS (Mu
Rashige and Skoog medium, White medium, B5 medium, V'W (V
The cells are cultured aseptically in a medium commonly used for plant tissue culture, such as Acin and Went) medium or Kano medium. These media have the compositions shown in Table 1, and can be selected from a variety of media depending on the type of plant to be cultured.

サトイモの場合は!、Is培地が好適であり、この培地
に植物ホルモンのα−ナフタレン酢酸とカイネチン又は
ベンジルアデニンを加えると良く生育する。この培養は
約60日間前後行い、生育した突然変異体を分離し、さ
らに培養を行い植物体を再生する。
In the case of taro! , Is medium is suitable, and it grows well when the plant hormones α-naphthalene acetic acid and kinetin or benzyladenine are added to this medium. This culture is carried out for about 60 days, and the grown mutants are separated and further cultured to regenerate the plant.

以上の方法により作出された突然変異体の収得割合は、
メチルニトロソウレアの5mM溶液の場合で51%、l
QmM溶液の場合で93%であった。この取得割合は突
然変異誘起物質の処理濃度により異なり、処理濃度の大
なる方が突然変異体の収得割合が増大する。
The yield rate of mutants created by the above method is
51% for a 5mM solution of methylnitrosourea, l
In the case of QmM solution, it was 93%. This acquisition rate varies depending on the treatment concentration of the mutagen, and the higher the treatment concentration, the greater the mutant acquisition rate.

以上の如くして作出した突然変異体を育苗床、素焼鉢で
育苗し、圃場に定植し通常の栽培を行う。
The mutants produced as described above are raised in seedbeds and clay pots, and then planted in a field and cultivated normally.

これにより、「八つ頭」の場合は、従来の親芋の形状は
全て塊状であったものが、本発明の方法で作出された突
然変異体の親芋の形状は整った円筒形9球形、扁球形ま
たは紡錐形であった。また、「ニゲ芋」の場合は、従来
の形状はやや長からやや丸の形状であったのに対し、本
発明の方法で作出された突然変異体の形状は、長から丸
であった。
As a result, in the case of ``Yatsuzu'', the shape of the conventional parent potato was all lumpy, but the shape of the mutant parent potato produced by the method of the present invention was a regular cylindrical shape, 9 spheres, and a flattened shape. It was spherical or fusiform. In addition, in the case of "Nigeimo", the conventional shape was slightly long to slightly round, whereas the shape of the mutant produced by the method of the present invention was long to round.

〔実施例〕〔Example〕

例  1 「八つ頭」150 個の芽を切取り、これを集めて2%
次亜塩素酸す) IJウム液に浸漬して殺菌し、解剖顕
微鏡下で茎頂部を切り取る。これをメチルニトロソウレ
アの5mMの水溶液に浸漬して40分間振盪する。その
後、茎頂部を取り出し、α−ナフタレン酢酸0.5mg
/j!を添加した)、IS培地20mβに植付け、60
日間培養する。その後、適湿条件で馴化を行い、得られ
た苗を分離し、120 株を育苗床に植え更に60日間
育苗する。
Example 1 Cut off 150 buds of “Yatsuzu” and collect them to make 2%
Sterilize by soaking in IJum solution (hypochlorous acid), and cut out the stem tip under a dissecting microscope. This is immersed in a 5mM aqueous solution of methylnitrosourea and shaken for 40 minutes. Then, remove the stem tip and add 0.5 mg of α-naphthalene acetic acid.
/j! ), planted on IS medium 20mβ, 60
Incubate for days. Thereafter, the seedlings were acclimatized under suitable humidity conditions, and the resulting seedlings were separated and 120 plants were planted in a nursery bed and raised for another 60 days.

かくして得られた幼苗を、広さ50m′の圃場に、畦幅
120 cm、  株間4Qcmに定植し、6力月間栽
培を行って親芋並びに子羊を収穫した。
The seedlings thus obtained were planted in a 50 m field with a ridge width of 120 cm and a plant spacing of 4 Q cm, and cultivation was carried out for 6 months to harvest parent potatoes and lambs.

例  2 メチルニトロソウレアの10mM溶液を使用するほかは
例1と同様の方法で、「八つ頭」の茎頂部をメチルニト
ロソウレア処理し、育苗、栽培を行い「八つ頭」を収穫
した。
Example 2 In the same manner as in Example 1 except that a 10 mM solution of methylnitrosourea was used, the shoot tips of "Yatsuzu" were treated with methylnitrosourea, seedlings were raised and cultivated, and "Yatsuzu" were harvested.

以上の例19例2の方法により収穫した「八つ頭」の親
芋と、対照としてメチルニトロソウレア処理を行わずに
収穫した「八つ頭」の親芋の形状及びシュート数を調査
した結果を第2表及び第3表に示す。
The results of investigating the shape and number of shoots of the "Yatsuzu" parent potatoes harvested by the method of Example 19 and Example 2 above and the "Yatsuzu" parent potatoes harvested without methylnitrosourea treatment as a control are as follows. It is shown in Table 2 and Table 3.

第2表 第3表 以上の結果より、親芋の形状は対照では全て塊状であっ
たが、本発明の例1及び例2のものは、紡錐形1円筒形
2球形、扁球形であった。そして、球形指数(直径/高
さ)は、対照のものでは1.1〜1.7、例1のもので
は0.4〜1.6、例2のものでは0.3〜2.3であ
った。また、その変異係数(PA準偏差/平均値で示さ
れる単位当たりの標準偏差)は、メチルニトロソウレア
の処理濃度が高いほど大であった。
From the results shown in Table 2 and Table 3, the shape of the parent potatoes in the control samples were all block-like, but in Examples 1 and 2 of the present invention, they were spinoidal, 1 cylindrical, 2 spherical, and oblate spheroidal. . And the sphericity index (diameter/height) was 1.1-1.7 for the control, 0.4-1.6 for example 1, and 0.3-2.3 for example 2. there were. Further, the variation coefficient (standard deviation per unit expressed as PA standard deviation/average value) was larger as the treatment concentration of methylnitrosourea was higher.

親芋のシュートの数は対照で6〜14、例1のもので1
〜30、例2のもので1〜40であり、その変異係数は
メチルニトロソウレアの濃度が高いほど大であった。親
芋が多数のシュートを有する場合は塊状であったが、−
本のシュートしか有しない場合は整った円筒形9球形、
紡錐形、扁球形であった。そして、親芋が一本のシュー
トを有する個体の百分率は、対照区では0%であるに対
し、例10区では13%、例2の区では70%であった
The number of shoots in parent potatoes is 6 to 14 in the control and 1 in case 1.
~30, and 1 to 40 for Example 2, and the variation coefficient was larger as the concentration of methylnitrosourea was higher. When the parent potato had many shoots, it was clumpy, but -
If you have only a book chute, a well-formed cylindrical nine-spherical shape,
It was fusiform and oblate. The percentage of individuals whose parent potatoes had a single shoot was 0% in the control plot, 13% in Example 10 plot, and 70% in Example 2 plot.

例  3 「ニゲ芋」の茎頂部をメチルニトロツウ1ノアの5mM
溶液で処理するほかは例1と同様の方法で、「ニゲ芋」
の茎頂部をメチルニトロソウレア処理し、育苗、栽培を
行い、収穫した子羊、孫芋の形状及び数を調査した。対
照としてメチルニトロソウレア処理を行わずに例3と同
様の方法で栽培した「ニゲ芋」の子午、孫芋の形状と対
比した。その結果を第4表に示す。
Example 3 5mM of 1 Noah of methyl nitrate was added to the top of the stem of “Nigeimo”.
In the same manner as in Example 1 except for treating with a solution, "Nigeimo"
The top of the stem was treated with methylnitrosourea, seedlings were raised and cultivated, and the shapes and numbers of the harvested lambs and grand potatoes were investigated. As a control, the shape of the meridian and grand potato of "Nige potato" grown in the same manner as in Example 3 without methylnitrosourea treatment was compared. The results are shown in Table 4.

第4表 以上の結果より、子羊、孫芋の形状において、対照のも
のが殆ど「やや長」から「やや丸」であったのに対し、
本発明の例3のものは「長」から「丸」までとなった。
From the results in Table 4 and above, the shapes of lamb and grand potatoes were mostly "slightly long" to "slightly round" for the controls, whereas
The shape of Example 3 of the present invention ranged from "long" to "round".

前記第2表で用いた「八つ頭」の親芋の形状分類を第1
図に示す。同図において、(a)は紡錐形、ら)は円筒
形、(C)は球形、(山は扁球形、(e)は塊状を示す
。なお、この分類は、社団法人日本種苗協会発行の昭和
59年度種苗特性分類調査報告書(昭和60年3月)に
記載の分類による。
The shape classification of the “Yatsuzu” parent potatoes used in Table 2 above is classified as
As shown in the figure. In the figure, (a) is cone-shaped, (a) is cylindrical, (C) is spherical, (mountains are oblate, and (e) is clumpy. This classification is published by the Japan Seedling Association. Based on the classification described in the 1985 Seedling Characteristics Classification Survey Report (March 1985).

また、本発明の方法により収穫された「八つ頭」の形状
の例を第2図、第3図に示し、本発明の処理を施さない
「八つ頭」の形状の例を第4図に示す。ここで、第2図
は本発明方法により親芋の形が改良された例であり、第
3図は親芋の形が改良されかつ子羊の数も多い例である
Further, examples of the shape of "Yatsuzu" harvested by the method of the present invention are shown in FIGS. 2 and 3, and FIG. 4 shows an example of the shape of "Yatsuzu" without the treatment of the present invention. Shown below. Here, FIG. 2 shows an example in which the shape of parent potatoes has been improved by the method of the present invention, and FIG. 3 shows an example in which the shape of parent potatoes has been improved and a large number of lambs have been produced.

〔発明の効果〕〔Effect of the invention〕

本発明はサトイモの人為的突然変異体を確実に作出し、
育種することができる方法であり、本発明方法によれば
、サトイモの形状が調理の際に除皮し易い円筒形、紡錐
形1球形、扁球形をなすものが高い確率で作出できる極
めて有用な発明である。
The present invention reliably creates artificial mutants of taro,
This is a method that allows for breeding, and the method of the present invention is extremely useful because it can produce taro with a high probability of having a cylindrical shape, a conical shape, and an oblate shape that are easy to remove during cooking. This is a great invention.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図はサトイモの形状の分類を示す図であり、第2図
及び第3図は本発明方法により収穫されたサトイモの外
観を示す図、第4図は対照のサトイモの外観を示す図で
ある。
Figure 1 is a diagram showing the classification of taro shapes, Figures 2 and 3 are diagrams showing the appearance of taro harvested by the method of the present invention, and Figure 4 is a diagram showing the appearance of a control taro. be.

Claims (1)

【特許請求の範囲】 1、サトイモの茎頂部を摘出し、これを突然変異誘起物
質で処理することにより変異を誘発させた後無菌培養に
より突然変異体を分離、育成することを特徴とするサト
イモの人為突然変異体作出法。 2、突然変異誘起物質がメチルニトロソウレアである特
許請求の範囲第1項記載のサトイモの人為突然変異体作
出法。 3、特許請求の範囲第1項記載の人為突然変異体作出法
によって作出されたサトイモの突然変異体を素材として
サトイモの新植物を育種することを特徴とするサトイモ
の育種法。
[Claims] 1. A taro plant characterized by removing the top of a taro plant, treating it with a mutagenic substance to induce mutations, and then isolating and growing mutants through sterile culture. Artificial mutant generation method. 2. The method for producing an artificial taro mutant according to claim 1, wherein the mutagenic substance is methylnitrosourea. 3. A method for breeding taro, which comprises breeding a new taro plant using a taro mutant produced by the artificial mutant production method as set forth in claim 1.
JP63189256A 1988-07-27 1988-07-27 Production of artificial mutant of colocasia antiquorum and breeding of colocasia antiquorum utilizing the same Granted JPH0239834A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP63189256A JPH0239834A (en) 1988-07-27 1988-07-27 Production of artificial mutant of colocasia antiquorum and breeding of colocasia antiquorum utilizing the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP63189256A JPH0239834A (en) 1988-07-27 1988-07-27 Production of artificial mutant of colocasia antiquorum and breeding of colocasia antiquorum utilizing the same

Publications (2)

Publication Number Publication Date
JPH0239834A true JPH0239834A (en) 1990-02-08
JPH0433416B2 JPH0433416B2 (en) 1992-06-03

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Family Applications (1)

Application Number Title Priority Date Filing Date
JP63189256A Granted JPH0239834A (en) 1988-07-27 1988-07-27 Production of artificial mutant of colocasia antiquorum and breeding of colocasia antiquorum utilizing the same

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Country Link
JP (1) JPH0239834A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995020313A1 (en) * 1994-01-31 1995-08-03 Consejo Superior De Investigaciones Cientificas Sunflower seeds and oil having a high stearic acid content
EP0934692A3 (en) * 1994-01-31 1999-08-25 Consejo Superior De Investigaciones Cientificas Sunflower seeds and plants having a high stearic acid content
US6891091B2 (en) 1994-01-31 2005-05-10 Consejo Superior De Investigaciones Cientificas Plant, seeds and oil with saturated triacylglycerol content and oil having a high stearic acid content

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995020313A1 (en) * 1994-01-31 1995-08-03 Consejo Superior De Investigaciones Cientificas Sunflower seeds and oil having a high stearic acid content
EP0934692A3 (en) * 1994-01-31 1999-08-25 Consejo Superior De Investigaciones Cientificas Sunflower seeds and plants having a high stearic acid content
US6486336B1 (en) 1994-01-31 2002-11-26 Consejo Superior De Investigaciones Cientificas Sunflower seeds and oil having a high stearic acid content
US6891091B2 (en) 1994-01-31 2005-05-10 Consejo Superior De Investigaciones Cientificas Plant, seeds and oil with saturated triacylglycerol content and oil having a high stearic acid content
US7009064B2 (en) 1994-01-31 2006-03-07 Consejo Superior De Investigaciones Cientificas Sunflower seeds and oil having a high stearic acid content
US7217875B2 (en) 1994-01-31 2007-05-15 Consejo Superior De Investigaciones Cientificas Sunflower seeds and oil having a high stearic acid content

Also Published As

Publication number Publication date
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