JPH02245129A - Enzymatically removing method for stain on stock - Google Patents
Enzymatically removing method for stain on stockInfo
- Publication number
- JPH02245129A JPH02245129A JP6403389A JP6403389A JPH02245129A JP H02245129 A JPH02245129 A JP H02245129A JP 6403389 A JP6403389 A JP 6403389A JP 6403389 A JP6403389 A JP 6403389A JP H02245129 A JPH02245129 A JP H02245129A
- Authority
- JP
- Japan
- Prior art keywords
- livestock
- dirt
- activity
- enzyme
- lipase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 21
- 244000144972 livestock Species 0.000 claims abstract description 38
- 102000004190 Enzymes Human genes 0.000 claims abstract description 23
- 108090000790 Enzymes Proteins 0.000 claims abstract description 23
- 229940088598 enzyme Drugs 0.000 claims abstract description 23
- 108010059892 Cellulase Proteins 0.000 claims abstract description 11
- 229940106157 cellulase Drugs 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 11
- 235000019626 lipase activity Nutrition 0.000 claims abstract description 6
- 230000001747 exhibiting effect Effects 0.000 claims abstract description 4
- 239000002689 soil Substances 0.000 claims description 6
- 241000283690 Bos taurus Species 0.000 abstract description 13
- 241001494479 Pecora Species 0.000 abstract description 4
- 241000282472 Canis lupus familiaris Species 0.000 abstract 1
- 102000053642 Catalytic RNA Human genes 0.000 abstract 1
- 108090000994 Catalytic RNA Proteins 0.000 abstract 1
- 108091092562 ribozyme Proteins 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 9
- 108090001060 Lipase Proteins 0.000 description 7
- 239000004367 Lipase Substances 0.000 description 7
- 102000004882 Lipase Human genes 0.000 description 7
- 235000019421 lipase Nutrition 0.000 description 7
- 238000004140 cleaning Methods 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 4
- 108010084185 Cellulases Proteins 0.000 description 3
- 102000005575 Cellulases Human genes 0.000 description 3
- 108010048733 Lipozyme Proteins 0.000 description 3
- 210000004209 hair Anatomy 0.000 description 3
- FCCDDURTIIUXBY-UHFFFAOYSA-N lipoamide Chemical compound NC(=O)CCCCC1CCSS1 FCCDDURTIIUXBY-UHFFFAOYSA-N 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- UYXTWWCETRIEDR-UHFFFAOYSA-N Tributyrin Chemical compound CCCC(=O)OCC(OC(=O)CCC)COC(=O)CCC UYXTWWCETRIEDR-UHFFFAOYSA-N 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000005802 health problem Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 101710130006 Beta-glucanase Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 108010056079 Subtilisins Proteins 0.000 description 1
- 102000005158 Subtilisins Human genes 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000001680 brushing effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 108010003855 mesentericopeptidase Proteins 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 108020004410 pectinesterase Proteins 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 108010075550 termamyl Proteins 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Landscapes
- Detergent Compositions (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
この発明は、家畜の体表に付着せる汚れその他の家畜に
由来する汚れを酵素によって除去する方法に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] This invention relates to a method for removing dirt adhering to the body surface of livestock and other dirt derived from livestock using enzymes.
本発明によって除去される汚れは、牛、羊、大その他の
家畜の体表に付着した排せつ物ならびに排せつ物と土壌
、牧草、飼料等との混合物、家畜の毛の中に蓄積したア
カ、汚れ、古くなった毛等である。（これらの汚れを、
以下、「家畜汚れ」と略称することがある。）
〔従来の技術〕
家畜の健康を維持し、順調な発育を図ることは家畜の飼
育上不可欠である。家畜の健康状態は主に用いる配合飼
料の栄養化によって左右されるが、その他に、飼育時の
衛生状態によっても影響を受ける。すなわち、家畜の体
表に付着した汚れは、しばしば衛生上の問題を生じさせ
、家畜の病気の原因となることはよく見られる現象であ
り、家畜の健康管理の妨げとなる。さらに、この体表付
着汚れは家畜の健康上の問題を引き起こすばかりではな
く、たとえば、乳牛の場合では牛乳の汚染の恐れ、肉牛
の場合では食肉への雑菌の感染等が起こり得、人体への
影響が懸念される。また、この家畜に付着した汚れは家
畜の商品価値の低下の原因となり、乳牛、肉牛の場合で
は１頭あたりこの汚れによって５〜１０％はどの価格の
低下を招く。Dirt that can be removed by the present invention includes excrement adhering to the body surface of cows, sheep, and other livestock, mixtures of excrement with soil, grass, feed, etc., scum, dirt, and old dirt accumulated in livestock hair. This is hair that has grown. (These stains,
Hereinafter, it may be abbreviated as "livestock stain". ) [Conventional technology] Maintaining the health of livestock and achieving smooth growth is essential in livestock breeding. The health condition of livestock is mainly influenced by the nutritional content of the compound feed used, but it is also influenced by the sanitary conditions during rearing. That is, dirt adhering to the body surface of livestock often causes sanitary problems and causes diseases in livestock, which is a common phenomenon and impedes health management of livestock. Furthermore, this dirt on the body surface not only causes health problems for livestock; for example, in the case of dairy cows, there is a risk of contamination of the milk, and in the case of beef cows, the meat may be infected with bacteria, and it poses a risk to the human body. There are concerns about the impact. In addition, this dirt attached to livestock causes a decrease in the commercial value of the livestock, and in the case of dairy cows and beef cattle, this dirt causes a 5 to 10% decrease in price per head.
従来、この家畜汚れを除去する方法としては、主にブラ
ッシングによるこすり落しが行なわれてきた。しかしな
がら、この方法では毛についた細かいアカなどは除去で
きても、家畜、特に牛の体表に固い塊状に付着した***
物、土壌、牧草などの汚れは除去することが不可能であ
った。また、−頭、−頭ブラッシングする手間は非常に
大きく、この方法は決して効率の良い方法とはいえない
。Conventionally, the main method for removing livestock stains has been to scrub them off by brushing. However, although this method can remove fine scales from hair, it is impossible to remove dirt such as excrement, soil, grass, etc. that has adhered to the body surface of livestock, especially cows, in hard clumps. In addition, the effort required to brush the -head and -head is extremely large, and this method cannot be said to be an efficient method.
その他、牛の場合において、飼料の栄養化を高め体表へ
の脂質の分泌を多くすることにより、糞塊等を体表に付
着しにくくする方法が行なわれている。しかしながら、
この方法は飼料のコストが高くなるばかりではなく、牛
の繁殖障害を引き起こし健康に異常を来たす。In addition, in the case of cattle, methods have been used to increase the nutritional content of feed and increase the secretion of lipids to the body surface, thereby making it difficult for feces and the like to adhere to the body surface. however,
This method not only increases the cost of feed, but also causes reproductive problems and health problems for the cows.
本発明の目的は、家畜汚れを効率よく除去することがで
きる方法を提供するにある。An object of the present invention is to provide a method that can efficiently remove livestock stains.
〔課題を解決するための手段〕
上記目的は、家畜の体表に付着した汚れその他の家畜に
由来する汚れをセルラーゼ活性および／またはリパーゼ
活性を示す酵素溶液と接触させることを特徴とする本発
明の方法によって達成される。[Means for Solving the Problems] The above object is achieved by the present invention, which is characterized in that dirt adhering to the body surface of livestock and other dirt derived from livestock are brought into contact with an enzyme solution exhibiting cellulase activity and/or lipase activity. This is achieved by the following method.
以下、本発明の方法をより詳しく説明する。The method of the present invention will be explained in more detail below.
牛、羊、その他の家畜の体表に付着した汚れその他の家
畜に由来する汚れは、本発明に従って、セルラーゼ活性
および／またはリパーゼ活性を示す酵素溶液と接触させ
ることによって容易に脱落する。Dirt adhering to the body surfaces of cows, sheep, and other livestock and other livestock-derived soils are easily removed by contacting with an enzyme solution exhibiting cellulase activity and/or lipase activity according to the present invention.
使用する酵素溶液としてはセルラーゼおよび／またはリ
パーゼの水溶液が用いられる。セルラーゼとしては、セ
ルロース鎖の結晶構造を破壊し、水和化し得る作用を示
すものであれば限定されるものではなく、その具体例と
してはセルザイム（ＣｅｌｌｕｚｙｍｅＨノボ社製）、
セルクラスト（Ｃｅｌｌｃｌａｓｔ；ノボ社製）等が挙
げられる。また、リパーゼとしては、主として脂肪酸の
グリセリドの加水分解または合成作用をなすものであれ
ば格別限定されることはなく、その具体例としてはりポ
ザイム（ＬｉｐｏｚｙｍｅＨノボ社製）、５Ｐ２８５
（ノボ社製）およびリポラーゼ（Ｌｉｐｏｌａｓｅ；ノ
ボ社製）等が挙げられる。これらのリパーゼおよびセル
ラーゼは２種類以上を同時に用いることもできる。The enzyme solution used is an aqueous solution of cellulase and/or lipase. Cellulases are not limited as long as they have the ability to destroy the crystal structure of cellulose chains and hydrate them; specific examples include Celluzyme (manufactured by Novo),
Examples include Cellclast (manufactured by Novo) and the like. In addition, the lipase is not particularly limited as long as it mainly acts to hydrolyze or synthesize glycerides of fatty acids, and specific examples include Lipozyme (manufactured by Novo), 5P285
(manufactured by Novo) and Lipolase (manufactured by Novo). Two or more types of these lipases and cellulases can also be used at the same time.
酵素溶液の濃度は、その使用条件、使用方法に依存して
変るが、一般に、セルラーゼは少くとも０、 Ｉ ＣＭ
Ｃ１ｌ／ｍｌ、好ましくは０．１〜１０ＣＭＣＵ／ｍｌ
、また、リパーゼは少くとも１０ＬＵ／ｍｌ、好ましく
は１０〜１００ＯＬＵ／ｄの濃度で用いる。ここで、Ｉ
ＣＭＣＵは、ＣＭＣを分解し、１分間に１マイクロモル
のグルコースに相当する還元糖を生成させる酵素活性量
と定義し、また、ＩＬＵは、トリブチリンを分解し、１
分間に１マイクロモルの遊離酪酸を生成させる酵素活性
量と定義する。The concentration of the enzyme solution varies depending on the conditions and method of use, but in general, the concentration of cellulase is at least 0, ICM.
C1l/ml, preferably 0.1-10CMCU/ml
Also, the lipase is used at a concentration of at least 10 LU/ml, preferably 10-100 OLU/d. Here, I
CMCU is defined as the amount of enzyme activity that decomposes CMC and produces reducing sugar equivalent to 1 micromole of glucose per minute, and ILU is defined as the amount of enzyme activity that decomposes tributyrin and produces 1 micromole of glucose per minute.
It is defined as the amount of enzyme activity that produces 1 micromole of free butyric acid per minute.
家畜の体表に付着した汚れと酵素溶液との接触は、酵素
溶液を家畜の付着汚れのうえに散布する手法によって行
われる。酵素溶液の散布量および散布方法は、溶液の酵
素活性濃度および家畜の付着汚れの程度に依存して変る
が、一つの代表例としては、牛−顧当り約２２の酵素溶
液を付着汚れのうえに一様に散布する操作を一日一回、
四日位繰り返す方法が採られる。この程度散布すること
により、１〜３週間経過すると付着汚れは自然に脱落す
る。The enzyme solution is brought into contact with the dirt adhering to the body surface of the livestock by a method of spraying the enzyme solution onto the dirt adhering to the livestock. The amount and method of spraying the enzyme solution will vary depending on the enzyme activity concentration of the solution and the degree of soiling on the livestock, but as a typical example, approximately 22 doses of the enzyme solution per cow are applied on the soiling. Once a day, spray evenly on the
A method of repeating the procedure for about four days is adopted. By spraying to this extent, the adhered dirt will naturally fall off after 1 to 3 weeks.
以下、リパーゼまたはセルラーゼによる家畜付着汚れの
除去の具体例を説明する。Hereinafter, a specific example of removing livestock stains using lipase or cellulase will be described.
牛の主として***物に由来する体表付着物を重さ３．０
〜３．５ ｇ 、 ３ｃｍ四方高さ１ｃｍはどに成形し
、試料とした。この試料２つを以下に示す各酵素の１％
水溶液Ｌｏｏｒｎ１に浸漬し、室温で３日間放置し、酵
素反応を行なった。なお、対照例として、試料を水（酵
素を含まない）に浸漬した群を設けた。Body surface deposits mainly derived from cow excrement weighing 3.0
A sample weighing ~3.5 g was molded into a 3 cm square and 1 cm high. Combine these two samples with 1% of each enzyme shown below.
It was immersed in an aqueous solution Loorn1 and left at room temperature for 3 days to perform an enzyme reaction. As a control example, a group in which the sample was immersed in water (not containing enzyme) was provided.
使用した酵素は以下のとおりである。The enzymes used are as follows.
■−−−素 賃−性一値セルザイム（Ｃ
ｅｌｌｕｚｙｍｅ） ２４００Ｔ ２，４５０
ＣＭＣＵ／ ｇセルザイム（Ｃｅｌｌｕｚｙｍｅ） １
５００ＣＰ １，５００ＣＭＣＵ／ ｇセルザイ
ム（Ｃｅｌｌｕｚｙｍｅ） ５Ｐ３５９ １．Ｏ
ＯＯＣＭＣＵ／ ｇセレフｏ （Ｃｅｒｅｆｌｏ） ２
０ＯＬペクチンエステラーゼ（Ｐｅｃｔｉｎｅｓｔｅｒ
ａｓｅ）フィニザイム（Ｆｉｎｉｚｙｍｅ） Ｌ
２００ＦＢＧ／ ｇリポザイム（Ｌｉｐｏｚ
ｙｍｅ；ノボ社製） ９０．０００ＬＵ／ ｇＳ
Ｐ２Ｂ５ ２９
，４００ＬＵ／ ｇアルカラーゼ（Ａｌｃａｌａｓｅ）
２． ＯＴエスペラーゼ（Ｂｓｐｅｒａｓｅ） ４．
ＯＴターマミル（Ｔｅｒｍａｍｙｌ）Ｌ−ＤＥＴ、タ
イプＡ １２８ＫＮＵ／ ｇ”Ｎｏ、１〜３はセル
ラーゼ（発明例）、Ｎｏ、７．８はりバーゼ（発明例）
であり、その他は比較例である。■---Primary price - one-value cellzyme (C
elluzyme) 2400T 2,450
CMCU/g Celluzyme 1
500CP 1,500CMCU/g Celluzyme 5P359 1. O
OOCMCU/ g celeflo (Cereflo) 2
0OL pectinesterase
ase) Finizyme L
200FBG/g Lipozyme (Lipoz
yme; manufactured by Novo) 90.000LU/gS
P2B5 29
,400LU/g Alcalase
2. OT esperase (Bsperase) 4.
OT Termamyl L-DET, Type A 128KNU/g"No. 1 to 3 are cellulase (invention example), No. 7.8 barase (invention example)
The others are comparative examples.
Ｎｏ、４．６はβ−グルカナーゼ、Ｎｏ、９．１０はプ
ロテアーゼ、Ｎｏ、１１はアミラーゼである。No. 4.6 is β-glucanase, No. 9.10 is protease, and No. 11 is amylase.
Ｎ（１１〜Ｎｏ、 １１の酵素は全てノボ（ＮＯＶＯ）
社製である。N (11~No, all 11 enzymes are NOVO)
It is made by a company.
酵素反応後に試料のかたさを、５００ｇ負荷のガラス棒
（直径５ｍｍ）の試料表面に対する１分間当りの貫入度
で評価した。結果は表１に示すとおりである。After the enzymatic reaction, the hardness of the sample was evaluated by the degree of penetration of a glass rod (5 mm in diameter) loaded with 500 g into the sample surface per minute. The results are shown in Table 1.
表 １ 表１にみられるとおり、セルラーゼ（１、２。Table 1 As seen in Table 1, cellulases (1, 2).
３）またはリパーゼ（７，８）の水溶液に浸漬した場合
には、対照および他の酵素水溶液に浸漬した場合と比較
して、付着物のかたさはかなり低下した。3) or lipase (7, 8), the hardness of the deposit was significantly reduced compared to the control and other enzyme aqueous solutions.
本発明に従って、家畜付着汚れにリパーゼおよび／また
はセルラーゼの水溶液を接触することにより、家畜汚れ
は自然にまたは容易に脱落する。According to the present invention, by contacting the livestock soil with an aqueous solution of lipase and/or cellulase, the livestock soil comes off naturally or easily.
従って、本発明の方法は、牛、羊、大その他の家畜、ペ
ット類の洗浄、家畜小屋等の洗浄、羊毛の洗浄、毛皮・
なめし皮工程における洗浄、鳥獣類の排せつ物の除去等
に利用できる。Therefore, the method of the present invention is suitable for cleaning cows, sheep, other livestock, pets, cleaning livestock sheds, etc., cleaning wool, cleaning fur, etc.
It can be used for cleaning in the tanning process, removing excrement from birds and animals, etc.
Claims (3)
る汚れをセルラーゼ活性および／またはリパーゼ活性を
示す酵素溶液と接触させることを特徴とする家畜***物
に由来する汚れを除去する方法。(1) A method for removing dirt derived from livestock excrement, which comprises contacting dirt deposited on the body surface of livestock and other livestock-derived soil with an enzyme solution exhibiting cellulase activity and/or lipase activity.
ラーゼ活性を示す請求項（１）記載の方法。(2) The method according to claim (1), wherein the enzyme solution exhibits a cellulase activity of at least 0.1 CMCU/ml.
性を示す請求項（１）記載の方法。(3) The method of claim (1), wherein the enzyme solution exhibits a lipase activity of at least 10 LU/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6403389A JPH02245129A (en) | 1989-03-17 | 1989-03-17 | Enzymatically removing method for stain on stock |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6403389A JPH02245129A (en) | 1989-03-17 | 1989-03-17 | Enzymatically removing method for stain on stock |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02245129A true JPH02245129A (en) | 1990-09-28 |
Family
ID=13246409
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6403389A Pending JPH02245129A (en) | 1989-03-17 | 1989-03-17 | Enzymatically removing method for stain on stock |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02245129A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6027657B2 (en) * | 1977-06-21 | 1985-06-29 | 花王株式会社 | 1-Alkoxycarbonyltricyclo[4.3.1.1↑2’↑5]undecane and its production method |
| JPS62220191A (en) * | 1986-06-11 | 1987-09-28 | Tadao Shiraishi | Encapsulated enzyme |
| JPS63161086A (en) * | 1986-12-10 | 1988-07-04 | ユニリーバー・ナームローゼ・ベンノートシヤープ | Enzyme-containing tableware washing and rinsing composition |
-
1989
- 1989-03-17 JP JP6403389A patent/JPH02245129A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6027657B2 (en) * | 1977-06-21 | 1985-06-29 | 花王株式会社 | 1-Alkoxycarbonyltricyclo[4.3.1.1↑2’↑5]undecane and its production method |
| JPS62220191A (en) * | 1986-06-11 | 1987-09-28 | Tadao Shiraishi | Encapsulated enzyme |
| JPS63161086A (en) * | 1986-12-10 | 1988-07-04 | ユニリーバー・ナームローゼ・ベンノートシヤープ | Enzyme-containing tableware washing and rinsing composition |
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