JPH01101880A - Rockwool cultivation of 'matsutake' mushroom - Google Patents
Rockwool cultivation of 'matsutake' mushroomInfo
- Publication number
- JPH01101880A JPH01101880A JP62259170A JP25917087A JPH01101880A JP H01101880 A JPH01101880 A JP H01101880A JP 62259170 A JP62259170 A JP 62259170A JP 25917087 A JP25917087 A JP 25917087A JP H01101880 A JPH01101880 A JP H01101880A
- Authority
- JP
- Japan
- Prior art keywords
- matsutake
- culture solution
- culture
- rockwool
- mycelia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000121220 Tricholoma matsutake Species 0.000 title claims abstract description 22
- 239000011490 mineral wool Substances 0.000 title claims abstract description 18
- 235000001674 Agaricus brunnescens Nutrition 0.000 title abstract description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 239000005556 hormone Substances 0.000 claims abstract description 3
- 229940088597 hormone Drugs 0.000 claims abstract description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 3
- 239000011707 mineral Substances 0.000 claims abstract description 3
- 238000012258 culturing Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- 235000000346 sugar Nutrition 0.000 claims description 2
- 150000008163 sugars Chemical class 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 9
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 abstract description 6
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 abstract description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 5
- 239000008103 glucose Substances 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 5
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 abstract description 3
- 229960000304 folic acid Drugs 0.000 abstract description 3
- 235000019152 folic acid Nutrition 0.000 abstract description 3
- 239000011724 folic acid Substances 0.000 abstract description 3
- 239000003617 indole-3-acetic acid Substances 0.000 abstract description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 abstract description 3
- 229960001763 zinc sulfate Drugs 0.000 abstract description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 abstract description 3
- 235000012907 honey Nutrition 0.000 abstract description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 abstract 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 abstract 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 235000010755 mineral Nutrition 0.000 abstract 1
- 229960002715 nicotine Drugs 0.000 abstract 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 abstract 1
- 239000001103 potassium chloride Substances 0.000 abstract 1
- 235000011164 potassium chloride Nutrition 0.000 abstract 1
- 229910000160 potassium phosphate Inorganic materials 0.000 abstract 1
- 235000011009 potassium phosphates Nutrition 0.000 abstract 1
- 229960000344 thiamine hydrochloride Drugs 0.000 abstract 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 abstract 1
- 239000011747 thiamine hydrochloride Substances 0.000 abstract 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 abstract 1
- 241000233866 Fungi Species 0.000 description 8
- 239000002609 medium Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 238000012136 culture method Methods 0.000 description 5
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 210000002268 wool Anatomy 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 240000006055 Dacrydium cupressinum Species 0.000 description 2
- 235000018782 Dacrydium cupressinum Nutrition 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000018646 Pinus brutia Species 0.000 description 2
- 235000013697 Pinus resinosa Nutrition 0.000 description 2
- NGPGDYLVALNKEG-UHFFFAOYSA-N azanium;azane;2,3,4-trihydroxy-4-oxobutanoate Chemical compound [NH4+].[NH4+].[O-]C(=O)C(O)C(O)C([O-])=O NGPGDYLVALNKEG-UHFFFAOYSA-N 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000012784 inorganic fiber Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- 229960003512 nicotinic acid Drugs 0.000 description 2
- 235000001968 nicotinic acid Nutrition 0.000 description 2
- 239000011664 nicotinic acid Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 240000000599 Lentinula edodes Species 0.000 description 1
- 235000001715 Lentinula edodes Nutrition 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、新素材ロックウール（岩綿）の物理的・化学
的性質に適応したマツタケ菌培養液を糖類・酵母・ミネ
ラル・ホルモンなどを用いて調製し、この希薄培養液に
粒状ロックウールを混入して、別に寒天培養したマツタ
ケ菌を接種して温度２３℃〜２４℃で振とう培養を行な
い、得られた液体菌糸を前記の濃厚培養液を吸収せしめ
たロックウールマットに接種して水分２５〜３０％温度
２１〜２２℃で継続培養を行ないマツタケ菌糸層を生成
する方法である。[Detailed Description of the Invention] The present invention involves preparing a Matsutake fungus culture solution adapted to the physical and chemical properties of the new material rock wool using sugars, yeast, minerals, hormones, etc. Granular rock wool was mixed into the culture solution, inoculated with Matsutake fungi that had been separately cultured on agar, and cultured with shaking at a temperature of 23°C to 24°C. In this method, a wool mat is inoculated and cultured continuously at a moisture content of 25 to 30% and a temperature of 21 to 22°C to produce a Matsutake mycelial layer.
マツタケ菌は生きたアカマツの細根に着”生して外生菌
根をつくり栄養を摂取して生きている。The Matsutake fungus attaches itself to the fine roots of living red pine trees, creates ectomycorrhizas, and lives by taking in nutrients.
マツタケ菌は活物寄生菌であるから、松根から切離され
るとカビや雑菌にすぐ犯されてしまう。マツタケはこの
菌の特性と弱さが障害となって、シイタケやその他のキ
ノコのように腐植性有機物に種菌を接種して増殖させる
ことは困難である。これまで長年にわたる各方面の実験
研究によって有効な培地と培養法が開発され寒天培養、
土壌培養、液体培養が示されているが、新素材の無機質
繊維を用いてマツタケ菌を培養する方法はまだ示されて
いない。Matsutake fungus is a living parasitic fungus, so if it is separated from the pine root, it will be immediately attacked by mold and other bacteria. Due to the characteristics and weakness of this fungus, it is difficult for matsutake to grow by inoculating the seed fungus into humic organic matter like shiitake and other mushrooms. Through many years of experimental research in various fields, effective media and culture methods have been developed, including agar culture,
Although soil culture and liquid culture have been shown, a method for culturing Matsutake fungi using a new material, inorganic fiber, has not yet been demonstrated.
本発明は１０数年来マツタケ菌の培養と松根への接種実
験をかさねてきた研究成果をもとにして、最近新しく製
造発売されているロックウールの特性に着目し、これを
培地としてマツタケ菌の培養に成功したのである。この
新しい素材の培地を使用して菌類培養を行なう場合は、
各菌類に適応する培養液の調製と各菌類に適応した培養
法を新規に開発しなければならない。The present invention is based on the research results of cultivating Matsutake fungi and inoculating them into pine roots over the past 10 years, and focused on the characteristics of rock wool, which has recently been newly manufactured and sold. It was successfully cultivated. When culturing fungi using this new medium,
It is necessary to prepare culture solutions suitable for each type of fungi and develop new culture methods suitable for each type of fungi.
新素材培地ロックウールの物理的・化学的性質を整理し
てみると次のとおりである。The physical and chemical properties of the new rock wool medium are summarized as follows.
Ａ、物理的性質
１、ケイ酸カルシウムを主原料とした無機質繊維である
。A. Physical properties 1. It is an inorganic fiber whose main raw material is calcium silicate.
２、均質で細い繊維（３〜７・）で、耐熱性（７００℃
）に優れている。2. Homogeneous and thin fibers (3 to 7), heat resistant (700℃)
) is excellent.
３、孔隙率が高く体積の９０％が空気である。3. High porosity and 90% of the volume is air.
４、無菌で品質が安定し、保水性と保温性に優れている
。4. It is sterile, has stable quality, and has excellent water and heat retention properties.
Ｂ、化学的性質
さらに、マツタケ菌の培養にあたってはマツタケ菌がど
んな成分を必要とするかを知る必要がある。B. Chemical properties Furthermore, in culturing Matsutake fungi, it is necessary to know what components the Matsutake fungus requires.
本発明ロックウール培養の大きな特長は、特殊な培養液
の調整と特殊な培養法を行なうことにあり、多くの培養
実験を繰返した結果衣の如き組成分と培養法が最も有効
であった。A major feature of the rock wool culture of the present invention is the preparation of a special culture solution and the use of a special culture method, and as a result of repeated many culture experiments, it was found that the composition and culture method similar to that of a batter were the most effective.
特殊培養液の組成
本発明では液体培養とウールマット固体培養との組合せ
が培養法の大きな特色である。まず、マツタケ菌の種菌
を得るため常法に従いグルコース２ｏＡ燥酵母５ｆ寒天
１８ノを水１，０ＯＯＣｃに溶解してＰ Ｈ５，０に調
製した培地を口径１８ｆｆ 試験管に１０−注入し温
熱殺菌後に原種歯を接種し、温度２３〜２４ ℃で３０
日間培養する。Composition of Special Culture Solution In the present invention, the combination of liquid culture and wool mat solid culture is a major feature of the culture method. First, in order to obtain the inoculum of Matsutake fungus, a culture medium prepared by dissolving 200 glucose, 500 g of dried yeast, 18 g of agar in 1,000 Cc of water and adjusting the pH to 5,0 was poured into a test tube with a diameter of 18 ff, followed by thermal sterilization. Inoculate the original tooth and incubate at a temperature of 23-24℃ for 30 minutes.
Incubate for days.
次に、これを種菌として、前記特殊培養液の４分の１の
希薄溶液を５００ｃｃ容三角フラスコに浅＜５Ｏｃｃ注
入し、同時に粒状ロックウール１ｆを混入し殺菌後接種
して温度 ２３〜２４℃に保たれた恒温室内の振とう器
にかけ毎分６０〜７０回の振とう培養を行ない、およそ
３０日後菌糸が粒状綿にまつわりつき増殖して全体がひ
とつに連なった状態で培養を終了する。Next, using this as an inoculum, a diluted solution of 1/4 of the above special culture solution was injected into a 500 cc Erlenmeyer flask at a shallow depth of <5 Occ, and at the same time, 1f of granular rock wool was mixed in, sterilized, and inoculated at a temperature of 23 to 24°C. Culture is carried out by shaking 60 to 70 times per minute in a shaker in a thermostatic chamber kept at a constant temperature, and after about 30 days, the mycelia cling to the granular cotton and multiply, and the culture is completed when the whole is connected as one.
次に、得られた綿状液体菌糸を種菌として、ロックウー
ルマット１００Ｘ１００Ｘ２５鱈２枚を培地とし、前記
特殊培養液をじゅうぶん吸収せしめた後に圧搾脱水し２
枚の間に挾むように接種して、温度２１〜２２℃で４０
〜５０日間培養する。上下のロックウールマットに菌糸
は増殖してマットは白色に変化し除々に菌糸層を生成す
る。Next, the resulting flocculent liquid mycelium was used as a seed fungus, two 100x100x25 cod rock wool mats were used as a culture medium, and after thoroughly absorbing the special culture solution, it was compressed and dehydrated.
Inoculate it between the sheets, and keep it at a temperature of 21 to 22℃ for 40 minutes.
Culture for ~50 days. Mycelium grows on the upper and lower rock wool mats, turning the mats white and gradually forming a mycelial layer.
本発明によって得られたロックウール培養のマツタケ菌
を、別のロックウール栽培により養成されたアカマツ苗
と接合して同時培養すれば、マツタケ菌根苗の養成が可
能となりマツタケの純粋人工栽培も実用化の段階に入り
革命的な技術進歩となる。If the rock wool-cultured Matsutake fungus obtained according to the present invention is combined with red pine seedlings cultivated through another rock wool cultivation method and co-cultivated, it becomes possible to cultivate Matsutake mycorrhizal seedlings, and pure artificial cultivation of Matsutake becomes practical. This is a revolutionary technological progress.
以下本発明の実験例を示せば次のとおりである。Experimental examples of the present invention are shown below.
本実験に使用したマツタケ原種菌は岩出菌学研究所が分
離培養したＭ２２６Ｌで、寒天培地は水道水１，０００
ｃｃにグルコース２０ｆエビオス５ｙ寒天１８ｆＰＨ
５，０で調製し、口径１８ｆｌ試験管に１０匡注入し、
温度１２０℃で１５分間温熱殺菌して冷却後１白金耳
量を接種、２４℃恒温器内に収容して３０日間培養した
。菌糸は培地上全面に０．５−位の厚さに増殖した。The Matsutake seed fungus used in this experiment was M226L isolated and cultured by Iwade Mycological Research Institute, and the agar medium was 1,000 ml of tap water.
cc glucose 20f Ebios 5y agar 18fPH
5.0, injected 10 squares into a 18 fl test tube,
After heat sterilization at a temperature of 120°C for 15 minutes and cooling, one platinum loopful was inoculated, placed in a 24°C incubator, and cultured for 30 days. Hyphae grew over the entire surface of the medium to a thickness of about 0.5 mm.
次に、５００ ｃｃ容三角フラスコに培地組成水１，０
００ｃｃ／’チミツ５ｆグルコース２０ｙエビオス５ｆ
酒石酸アンモニウム１ｙ 燐酸−力ＩＪ １ Ｆ
塩化力ＩＪ ０．２１ 硫酸亜鉛１．０■ニコチン酸
０．５■ 葉酸０．５■ インドール醋酸０．０１■Ｐ
Ｈ４，５を調製し４分の１に薄めて５０ ｃｃを注入
し、同時に粒状ロックウール１ｆを混入して高圧釜で１
２０℃で温熱殺菌する。Next, in a 500 cc Erlenmeyer flask, add water with a medium composition of 1.0
00cc/'Chimitsu 5f Glucose 20y Ebios 5f
Ammonium tartrate 1y Phosphoric acid IJ 1 F
Chloride power IJ 0.21 Zinc sulfate 1.0 ■ Nicotinic acid 0.5 ■ Folic acid 0.5 ■ Indole acetic acid 0.01 ■ P
Prepare H4,5, dilute it to 1/4, inject 50 cc, mix 1f of granular rock wool at the same time, and boil it in a high-pressure cooker.
Heat sterilize at 20°C.
冷却後１８ｆｌ試験管１本分の菌糸を無菌箱中で接種し
、２４℃恒温室内の振とう台に乗せて毎分６０回で振と
う培養した。３１日後に培養液中いっばいに綿が広がっ
た状態で培養を終了した。After cooling, one 18 fl test tube of mycelia was inoculated in a sterile box, placed on a shaking table in a thermostatic chamber at 24° C., and cultured with shaking at 60 times per minute. After 31 days, the culture was terminated when the cotton was spread all over the culture solution.
次に、ロックウールマット１００ｘ１００ｘ２５鰭２枚
を培地としてこれに培地組成水 １．０００ ｃｃハチ
ミツ１０ｆグルコース２０ｆエビオス５Ｆ酒石酸アンモ
ニウム１ｆ燐酸−カリ１ノ塩化力＋） ０．２ ｙ硫酸
亜鉛１．０■ニコチン酸０．５■葉酸０．５ｆｆｉｇ
インドール醋酸０．０１■ Ｐ Ｈ４，５を調製して
この培養液をじゅうぶん吸収せしめた後、培地の水分が
２５％になるよう手押し圧搾器で余剰水分を除去した。Next, use two rock wool mats 100x100x25 fins as a medium and add the following medium composition: Water 1.000 cc Honey 10f Glucose 20f Ebios 5F Ammonium tartrate 1f Phosphoric acid-potassium 1 monochloride +) 0.2 y Zinc sulfate 1.0 ■ Nicotinic acid 0.5■ Folic acid 0.5ffig
After preparing indole acetic acid 0.01μPH 4.5 and sufficiently absorbing the culture solution, excess water was removed using a hand press so that the water content of the culture medium was 25%.
培地マット２枚の上下の間に挾むようにして液体種菌５
０ ｃｃを接種し温度２１℃の恒温器内で４０日間培養
した。この間水分の補給はほとんど不要であるが測定水
分２０％以下となった場合はスポイトで点滴する。４０
日後には菌糸はウールマットに侵入増殖して白色となり
、５０日後には菌糸層が認められるようになった。Place liquid seed culture 5 between the top and bottom of two medium mats.
0 cc was inoculated and cultured for 40 days in an incubator at a temperature of 21°C. During this time, there is almost no need to replenish water, but if the measured water content is less than 20%, drip infusion with a dropper. 40
After a few days, the mycelium invaded and multiplied in the wool mat, turning it white, and after 50 days, a mycelium layer became visible.
Claims (1)
含有する希薄培養液に粒状ロックウールを混入して温度
２３〜２４℃で振とう培養を行ない、得られた液体菌糸
を前記の濃厚培養液を吸収せしめたロックウールマット
に接種し水分２５〜３０％温度２１〜２２℃で培養を継
続して菌糸層を生成することを特長とするマツタケ菌培
養法。Matsutake fungus is cultured with shaking at a temperature of 23-24℃ by mixing granular rock wool into a dilute culture solution containing sugars, yeast, minerals, hormones, etc., and the resulting liquid mycelium is mixed with the above-mentioned concentrated culture solution. A method for cultivating Matsutake fungus, which comprises inoculating an absorbed rock wool mat and continuing culturing at a moisture content of 25 to 30% and a temperature of 21 to 22°C to generate a mycelial layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62259170A JPH01101880A (en) | 1987-10-14 | 1987-10-14 | Rockwool cultivation of 'matsutake' mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62259170A JPH01101880A (en) | 1987-10-14 | 1987-10-14 | Rockwool cultivation of 'matsutake' mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01101880A true JPH01101880A (en) | 1989-04-19 |
Family
ID=17330330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62259170A Pending JPH01101880A (en) | 1987-10-14 | 1987-10-14 | Rockwool cultivation of 'matsutake' mushroom |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01101880A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0220283A (en) * | 1988-07-08 | 1990-01-23 | Kibun Kk | Method for increasing fragrant component of matsutake mushroom |
| JPH03195421A (en) * | 1989-12-25 | 1991-08-27 | Kyujiro Koyama | Yield increasing agent of mushrooms and culture thereof |
| EP1226748A1 (en) * | 2001-01-26 | 2002-07-31 | The University of Tokyo | Method of forming an artificial shiro of matsutake |
-
1987
- 1987-10-14 JP JP62259170A patent/JPH01101880A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0220283A (en) * | 1988-07-08 | 1990-01-23 | Kibun Kk | Method for increasing fragrant component of matsutake mushroom |
| JPH03195421A (en) * | 1989-12-25 | 1991-08-27 | Kyujiro Koyama | Yield increasing agent of mushrooms and culture thereof |
| EP1226748A1 (en) * | 2001-01-26 | 2002-07-31 | The University of Tokyo | Method of forming an artificial shiro of matsutake |
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