JP7254349B2 - 生体分子標的の光検知システム及び方法 - Google Patents
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Description
本出願は、2016年10月26日に出願された“光検知を用いた自動核酸検出及び定量”と題する米国仮特許出願第62/413144号の利益及び優先権を主張し、その全ての内容を参照により本明細書に援用する。
B ナノ粒子結合部位
X 軸線
Y 軸線
10 ポータブルアッセイシステム
18 ロータ
18P ポート
20 使い捨てアッセイカートリッジ
21 フローセル
22 カートリッジボディ
22B シリンジバレル
24 線形アクチュエータ
26 プランジャシャフト
28 エラストマープランジャ
30 中央チャンバ
32 アッセイチャンバ
34 アッセイチャンバ
40 チャネル
42 チャネル
44 下部パネル
50 開口部
70 センサシステム
72 撮像装置
74 ピクセルアレイ
75 レンズ/対物レンズ
76 アレイ回路
78 フローセル
80 面
82 機能化面
84 捕捉プローブ
86 標的分子
88 磁石
90 方法
92~104 方法の工程
110 磁性粒子
110A 磁性粒子
110B 磁性粒子
112 磁気コア
114 ナノ粒子コーティング
116 磁性体
118 動き
120 動き
122 ナノ粒子
124 拡大ナノ粒子
126 光源
128 光線
130 光
132 散乱の兆候(画像)
134 散乱の兆候(画像)
140 光学センサシステム
142 撮像装置
144 対物レンズ/レンズ
146 フローセル
147 分配線
148 光源
150 磁石
152 アクチュエータ
160 分析システム
162 ベース
164 ヘッド
166 側面
167 側面
168 ミラー
170 光路
180 センサシステム
182 プリズム基板
184 面
186 膜
188 光源
190 光線
192 検出器
194 光
Claims (18)
- サンプル内の標的分子検出システムであって、該システムは、
ピクセルアレイを含む撮像装置と、
標的分子を結合するように構成された複数の捕捉プローブと、機能化面に対向する少なくとも1つの光学的に透明な光透過面とを備える、前記機能化面を備えるフローセルであって、各標的分子は、磁性粒子と組み合わされて、磁性の標的分子を画定し、前記フローセルは、前記面間に磁性の前記標的分子の溶液を含む、フローセルと、
前記機能化面の反対側に配置された磁石であって、前記磁石は、磁性の前記標的分子を前記機能化面に向けるように構成され、磁性の前記標的分子の一部が前記捕捉プローブと結合して、結合した磁性の標的分子を画定する、磁石と、
一次光線を前記フローセルの前記光透過面と前記機能化面との間および結合した磁性の前記標的分子に向けるように構成された光源であって、前記フローセルが、前記一次光線が前記光源から撮像装置に直接拡散するのを防ぐように、かつ、結合した磁性の前記標的分子によって反射された二次光線を放出するようにさらに構成された、光源と、
前記磁石に結合されたアクチュエータであって、前記磁石を前記機能化面に近づけたり遠ざけたりするように構成されたアクチュエータとを、備え、
前記アクチュエータは、前記磁石を前記フローセルの前記機能化面から遠ざけるように動かし、前記機能化面が、前記複数の捕捉プローブのうちの少なくともいくつかを使用して、1回の操作で結合した磁性の前記標的分子を捕捉し、
前記磁石を使用する第2の操作において、複数の非結合の磁性の標的分子が前記フローセルから排出され、
前記撮像装置は、前記少なくとも1つの光学的に透明な光透過面の反対側に配置され、第3の操作において、前記撮像装置の前記ピクセルアレイを使用して前記二次光線のそれぞれを捕捉し、前記サンプル中の結合した磁性の前記標的分子の存在および量を検出する、システム。 - 前記撮像装置が暗視野像を取り込むように構成される、請求項1に記載のシステム。
- 前記標的分子が前記機能化面に結合したとき前記標的分子がナノ粒子に結合するように構成され、前記ナノ粒子は光線を前記撮像装置へ反射するように構成される、請求項1に記載のシステム。
- 前記ナノ粒子が金ナノ粒子である、請求項3に記載のシステム。
- 前記ナノ粒子はさらに拡大ナノ粒子の成長のための核形成部位として働くように構成される、請求項3に記載のシステム。
- 前記撮像装置と前記フローセルの間に配置されたレンズをさらに備える、請求項1に記載のシステム。
- 請求項1に記載のサンプル内の標的分子検出システムを用いた、サンプル内の標的分子検出方法であって、
標的分子を磁性粒子に結合するステップと、
前記磁石を介して前記磁性粒子及び標的分子を前記機能化面に向けるステップと、
前記標的分子を前記複数の捕捉プローブの一つに結合するステップと、
ナノ粒子を前記標的分子に結合するステップと、
前記光源からの光線を前記ナノ粒子に向けるステップと、
前記撮像装置で前記ナノ粒子からの光を取り込むステップと、
前記標的分子を検出するために前記ナノ粒子からの前記光を分析するステップと、
前記アクチュエータは、前記磁石を前記フローセルの前記機能化面から遠ざけるように動かし、前記機能化面が、前記複数の捕捉プローブのうちの少なくともいくつかを使用して、1回の操作で結合した磁性の前記標的分子を捕捉するステップと、
前記磁石を使用する第2の操作において、複数の非結合の磁性の標的分子が前記フローセルから排出するステップと、
前記撮像装置は、前記少なくとも1つの光学的に透明な光透過面の反対側に配置され、第3の操作において、前記撮像装置の前記ピクセルアレイを使用して前記二次光線のそれぞれを捕捉し、前記サンプル中の結合した磁性の前記標的分子の存在および量を検出するステップと、
を含む、方法。 - 前記光を取り込むステップは暗視野像を取り込むステップを含み、前記光を分析するステップは前記暗視野像に取り込まれた光のスポットを定量化するステップを含む、請求項7に記載の方法。
- 拡大ナノ粒子を成長させるステップをさらに含み、前記ナノ粒子はさらに核形成部位として働くように構成される、請求項7に記載の方法。
- 前記ナノ粒子を結合するステップは前記ナノ粒子を前記標的分子に直接結合するステップを含む、請求項7に記載の方法。
- 前記ナノ粒子を結合するステップは前記標的分子に結合した前記磁性粒子の結合部位に前記ナノ粒子を結合するステップを含む、請求項7に記載の方法。
- 前記光線を前記ナノ粒子に向けるステップは前記撮像装置への光線の拡散を妨げるステップを含む、請求項7に記載の方法。
- 前記標的分子を前記磁性粒子からほどくように前記標的分子を変性するステップをさらに含む、請求項7に記載の方法。
- 前記磁性粒子は、磁性体と、前記磁性粒子を前記標的分子に結合するように構成された結合部位とを備える、請求項7に記載の方法。
- 前記磁性粒子はさらに金コーティングを備える、請求項14に記載の方法。
- 前記磁性粒子はさらにナノ粒子結合部位を備える、請求項14に記載の方法。
- 請求項1に記載のサンプル内の標的分子検出システムを用いた、サンプル内の標的分子検出方法であって、
標的分子を磁性粒子に結合するステップと、
前記磁石を介して前記磁性粒子及び標的分子を前記機能化面に向けるステップと、
前記標的分子を前記複数の捕捉プローブの一つに結合するステップと、
前記光源からの光線を前記磁性粒子に向けるステップと、
前記撮像装置で前記磁性粒子からの光を取り込むステップと、
前記標的分子を検出するために前記磁性粒子からの光を分析するステップと、
前記アクチュエータは、前記磁石を前記フローセルの前記機能化面から遠ざけるように動かし、前記機能化面が、前記複数の捕捉プローブのうちの少なくともいくつかを使用して、1回の操作で結合した磁性の前記標的分子を捕捉するステップと、
前記磁石を使用する第2の操作において、複数の非結合の磁性の標的分子が前記フローセルから排出するステップと、
前記撮像装置は、前記少なくとも1つの光学的に透明な光透過面の反対側に配置され、第3の操作において、前記撮像装置の前記ピクセルアレイを使用して前記二次光線のそれぞれを捕捉し、前記サンプル中の結合した磁性の前記標的分子の存在および量を検出するステップと、
を含む、方法。 - 前記磁性粒子が鉄-金の複合材料である、請求項17に記載の方法。
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Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004275187A (ja) | 2003-02-27 | 2004-10-07 | Institute Of Physical & Chemical Research | 金コロイド粒子を用いるdnaの配列検知方法、ターゲットdnaの末端一塩基変異検出方法、遺伝子診断方法 |
JP2005520125A (ja) | 2001-05-25 | 2005-07-07 | ノースウエスタン ユニバーシティ | 非合金のコアシェル型ナノ粒子 |
US20100120132A1 (en) | 2006-03-31 | 2010-05-13 | Intel Corporation | Bioassays by direct optical detection of nanoparticles |
JP2012521219A (ja) | 2009-03-24 | 2012-09-13 | ユニバーシティ オブ シカゴ | SlipChip装置および方法 |
JP2013503352A (ja) | 2009-08-31 | 2013-01-31 | エムバイオ ダイアグノスティクス,インコーポレイティド | 統合されたサンプル調製及び検体検出 |
US20140308680A1 (en) | 2011-10-14 | 2014-10-16 | Koninklijke Philips N.V. | Method for detection of coagulation activity and biomarkers |
US20150017258A1 (en) | 2012-01-31 | 2015-01-15 | American University Of Cairo (Auc) | Direct detection of disease biomarkers in clinical specimens using cationic nanoparticle-based assays & versatile and green methods for synthesis of anisotropic silver nanostructures |
US20150037818A1 (en) | 2013-07-01 | 2015-02-05 | University Of Memphis Research Foundation | Iron oxide-gold core-shell nanoparticles and uses thereof |
WO2015031691A1 (en) | 2013-08-28 | 2015-03-05 | Cellular Research, Inc. | Massively parallel single cell analysis |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04102062A (ja) * | 1990-08-22 | 1992-04-03 | Hitachi Ltd | 粒子免疫側定法 |
WO2002071029A1 (en) * | 2001-03-02 | 2002-09-12 | Waters Investments Limited | Fluorescence detector geometry |
US7645574B2 (en) | 2003-01-23 | 2010-01-12 | Integrated Nano-Technologies, Llc | Methods of metallizing nucleic acid molecules and methods of attaching nucleic acid molecules to conductive surfaces |
WO2007095279A2 (en) * | 2006-02-14 | 2007-08-23 | University Of Florida Research Foundation, Inc. | Dual nanoparticle assay for detection and separation of biological species |
US7811810B2 (en) * | 2007-10-25 | 2010-10-12 | Industrial Technology Research Institute | Bioassay system including optical detection apparatuses, and method for detecting biomolecules |
GB0904934D0 (en) * | 2009-03-23 | 2009-05-06 | Geneseqe As | Method and apparatus for detecting molecules |
KR101121556B1 (ko) * | 2009-12-09 | 2012-03-06 | 한국과학기술원 | 금 나노입자 성장에 의한 형광저하를 이용한 고감도 분석물 검출방법 |
-
2017
- 2017-10-26 AU AU2017347825A patent/AU2017347825C1/en not_active Ceased
- 2017-10-26 US US16/345,175 patent/US20190285639A1/en not_active Abandoned
- 2017-10-26 WO PCT/US2017/058559 patent/WO2018081440A1/en unknown
- 2017-10-26 CA CA3042036A patent/CA3042036A1/en active Pending
- 2017-10-26 EP EP17864944.8A patent/EP3532821A4/en not_active Withdrawn
- 2017-10-26 JP JP2019522435A patent/JP7254349B2/ja active Active
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005520125A (ja) | 2001-05-25 | 2005-07-07 | ノースウエスタン ユニバーシティ | 非合金のコアシェル型ナノ粒子 |
JP2004275187A (ja) | 2003-02-27 | 2004-10-07 | Institute Of Physical & Chemical Research | 金コロイド粒子を用いるdnaの配列検知方法、ターゲットdnaの末端一塩基変異検出方法、遺伝子診断方法 |
US20100120132A1 (en) | 2006-03-31 | 2010-05-13 | Intel Corporation | Bioassays by direct optical detection of nanoparticles |
JP2012521219A (ja) | 2009-03-24 | 2012-09-13 | ユニバーシティ オブ シカゴ | SlipChip装置および方法 |
JP2013503352A (ja) | 2009-08-31 | 2013-01-31 | エムバイオ ダイアグノスティクス,インコーポレイティド | 統合されたサンプル調製及び検体検出 |
US20140308680A1 (en) | 2011-10-14 | 2014-10-16 | Koninklijke Philips N.V. | Method for detection of coagulation activity and biomarkers |
US20150017258A1 (en) | 2012-01-31 | 2015-01-15 | American University Of Cairo (Auc) | Direct detection of disease biomarkers in clinical specimens using cationic nanoparticle-based assays & versatile and green methods for synthesis of anisotropic silver nanostructures |
US20150037818A1 (en) | 2013-07-01 | 2015-02-05 | University Of Memphis Research Foundation | Iron oxide-gold core-shell nanoparticles and uses thereof |
WO2015031691A1 (en) | 2013-08-28 | 2015-03-05 | Cellular Research, Inc. | Massively parallel single cell analysis |
Non-Patent Citations (1)
Title |
---|
WERTS, Martinus H. V.,Optical microscopy and spectroscopy of analyte-sensitive functionalized gold nanoparticles in microfluidic systems,Proc. SPIE,2013年,8595, 85950W,pp.1-11 |
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