JP6832954B2 - 培養機器 - Google Patents
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- JP6832954B2 JP6832954B2 JP2018556108A JP2018556108A JP6832954B2 JP 6832954 B2 JP6832954 B2 JP 6832954B2 JP 2018556108 A JP2018556108 A JP 2018556108A JP 2018556108 A JP2018556108 A JP 2018556108A JP 6832954 B2 JP6832954 B2 JP 6832954B2
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
- G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/80—Indicating pH value
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Description
培養機器を用いて細胞の培養を行う際は、通常、細菌の侵入等を防ぐために各容器の開口部を閉じるように蓋が設置される。細胞は、30〜35℃程度の温度で培養されるため、培養液が蒸発して上記蓋が曇ることが多い。蓋が曇っていると、容器内部の観察に支障をきたし、例えば、培養液の濁度測定を正確に行うことが困難となる。
[培養機器の構成]
図1は、実施例1に係る培養機器1の構成を説明するための図である。培養機器1は、蓋11とマイクロプレート12と中間プレート13とを備える。図1(a)は、蓋11、マイクロプレート12、中間プレート13のマイクロプレート12と接する面を拡大した一部及びマイクロプレート12が備える容器14を示す斜視図である。
マイクロプレート12は、上部が開口した複数の容器14を備える。容器14は培養液等を収容する。図1(a)に示した例では、マイクロプレート12は、12列×8行に配列された合計96個の容器14を備える。
実施例1に係る培養機器1では、蓋11と中間プレート13とが、それぞれ透光性を有する。また、培養液18の液面の位置を凸部15の面15Aが浸漬する位置にした場合、凸部15の面15Aが蒸発した培養液18の結露により曇ることがない。さらには、蓋11は、中間プレート13の凸部15の上方部分に位置する箇所が曇らない。そのため、実施例1に係る培養機器1を使用した場合、蓋11及び中間プレート13をマイクロプレート12に重ねた状態であっても上記面15Aを介して容器14の内部を良好に観察することができる。
図2は、濁度を測定するための光学系Sを説明するための図である。光学系Sは、光源(Light Source)19とダイクロイックミラー20と第1ホトダイオード(PD)21と第2ホトダイオード(PD)22と培養機器1とを備える。
上記の説明において、マイクロプレート12は96個の容器14を備えるとしたが、マイクロプレート12が備える容器14の数は上記値に限定されない。マイクロプレート12は任意の数の容器14を備えることができる。
蓋11と複数の容器14の底面14Aと中間プレート13とは、光学特性が略同じであることが望ましい。具体的には、蓋11と複数の容器14の底面14Aと中間プレート13とは、屈折率が同じ材料で形成されてもよい。このようにすると、光が構成要素間で屈折して観察対象が歪むこと及び濁度測定の精度が低くなることを防げる。
上記の説明では中間プレート13の面は蓋11と略接するとしたが、中間プレート13の面は必ずしも全面が蓋11と接する必要はない。蓋11及び中間プレート13の形状は、蒸発した培養液18が付着して蓋11が結露するのを防げる形状であればよい。
実施例1の培養機器1は、凸部15及び貫通口16を有する中間プレート13を蓋11とマイクロプレート12との間に挟む構成であった。これに対し、実施例2の培養機器は、中間プレート13を挟む代わりに容器14に透光性を有する部品を取り付ける点で実施例1の培養機器1と異なる。
実施例2の培養機器2では、透光性の部品23が容器14内部に接着されていた。実施例3の培養機器3は、容器14内部に透光性の部品24が圧入される又は嵌め込まれる点が実施例2の培養機器2と異なる。
図7は、容器14に圧入する部品24の別の例を示す図である。図7に示した部品26は、円柱状の中央部26aから四方に突出部26bが伸びている。つまり、部品26は部品24とは異なり2方向に突っ張る構造となっており、容器14が有する開口部17は四つに区分されている。部品26が圧入された容器14は、上記開口部17から液体の注入及び吸引が可能である。
培養機器は、容器14に凍結した抗菌剤27が収容された状態で提供されてもよい。その場合、冷凍保管していた培養機器を室温にて抗菌剤27を溶解し、必要な培養液18及び検体を添加することによって、薬剤感受性試験に適した媒体を調製することができる。なお、抗菌剤27のみならず培養液18及びその他の薬剤も凍結した状態で収容されてもよい。
11…蓋
12A、B、C…マイクロプレート
13…中間プレート
14…容器
15…凸部
16…貫通口
17…開口部
18…培養液
20…ダイクロイックミラー
23、24、26…部品
25…抗菌剤
26a…中央部
26b…突出部
27…凍結状態の抗菌剤
本明細書で引用した全ての刊行物、特許文献はそのまま引用により本明細書に組み入れられるものとする。
Claims (7)
- 底面が透光性を有し、開口部を有するように上端から内部に伸びる透光性の部品が取り付けられた、培養液を収容するための複数の容器を備えるマイクロプレートと、
透光性を有し、前記マイクロプレートの上面を覆う蓋と、
を備え、
前記部品は前記容器の底面と略平行な二面を有し、前記二面のうち一方の面は前記マイクロプレートの上面と同じ高さ又はそれよりも高い位置に位置し、
前記部品の大きさは、使用時に前記二面のうち他方の面が前記容器に収容された前記培養液に浸漬するように調節される、
培養機器。 - 請求項1に記載の培養機器において、
前記蓋と前記容器の底面と前記部品とは光学特性が略同じである、
培養機器。 - 請求項1に記載の培養機器において、
前記容器のそれぞれの内部には、凍結乾燥状態の抗菌剤が収容されている、
培養機器。 - 請求項1に記載の培養機器において、
前記容器のそれぞれの内部には、凍結状態の抗菌剤又は培養液成分が収容されている、
培養機器。 - 請求項1に記載の培養機器において、
前記マイクロプレートは前記容器を24個以上備える、
培養機器。 - 請求項1に記載の培養機器において、
前記部品は前記容器に接着されて取り付けられる、
培養機器。 - 請求項1に記載の培養機器において、
前記部品は前記容器に圧入されて取り付けられる、
培養機器。
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Application Number | Priority Date | Filing Date | Title |
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PCT/JP2016/087301 WO2018109886A1 (ja) | 2016-12-14 | 2016-12-14 | 培養機器 |
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JPWO2018109886A1 JPWO2018109886A1 (ja) | 2019-10-24 |
JP6832954B2 true JP6832954B2 (ja) | 2021-02-24 |
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US (1) | US11618873B2 (ja) |
EP (1) | EP3556844B1 (ja) |
JP (1) | JP6832954B2 (ja) |
CN (1) | CN110023480B (ja) |
WO (1) | WO2018109886A1 (ja) |
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KR102193016B1 (ko) * | 2018-12-17 | 2020-12-18 | 엠비디 주식회사 | 바이오 칩용 필라 구조체 |
CN110992784B (zh) * | 2019-11-20 | 2021-11-09 | 万有造诣(深圳)教育科技有限公司 | 一种教育科技用科学试验装置 |
EP4384800A1 (en) * | 2021-08-08 | 2024-06-19 | cytena Bioprocess Solutions co., Ltd. | Image acquisition system for acquiring an image of a liquid sample |
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US4720463A (en) * | 1985-03-01 | 1988-01-19 | Sherwood Medical Company | Automated microbiological testing apparatus |
JP2876080B2 (ja) | 1989-09-16 | 1999-03-31 | 本田技研工業株式会社 | 自動2輪車のエンジンカバー装置 |
US5141718A (en) * | 1990-10-30 | 1992-08-25 | Millipore Corporation | Test plate apparatus |
CA2142868C (en) * | 1992-08-21 | 2001-07-24 | Yuichi Kinoshita | Chemical and microbiological test kit |
US5366893A (en) * | 1993-01-13 | 1994-11-22 | Becton, Dickinson And Company | Culture vessel |
US6153400A (en) | 1999-03-12 | 2000-11-28 | Akzo Nobel N.V. | Device and method for microbial antibiotic susceptibility testing |
DE10204531A1 (de) * | 2002-02-01 | 2003-08-21 | Inst Chemo Biosensorik | Deckelelement |
JP3104790U (ja) | 2004-04-22 | 2004-10-14 | 有限会社金沢大学ティ・エル・オー | 顕微鏡用細胞培養器 |
US8058060B2 (en) * | 2005-06-10 | 2011-11-15 | Nunc A/S | Culture insert carrier, culture insert and culture insert system |
JP2007300853A (ja) * | 2006-05-11 | 2007-11-22 | Nikon Corp | 培養容器および自動培養装置 |
US20090082600A1 (en) * | 2007-06-06 | 2009-03-26 | Shengde Zhou | Native homoethanol Pathway for ethanol production in E. coli |
CN102719352B (zh) * | 2012-06-06 | 2014-01-29 | 西安交通大学 | 一种用于制备微阵列细胞芯片的细胞芯片片基及制备方法 |
CN103013829B (zh) * | 2012-12-28 | 2015-04-22 | 江南大学 | 深孔细胞培养板板盖的结构 |
US9790465B2 (en) * | 2013-04-30 | 2017-10-17 | Corning Incorporated | Spheroid cell culture well article and methods thereof |
JP5768174B1 (ja) * | 2014-06-24 | 2015-08-26 | 日本写真印刷株式会社 | 培養容器 |
CN203960224U (zh) * | 2014-08-06 | 2014-11-26 | 青岛易邦生物工程有限公司 | 新型细胞培养板 |
JP6479368B2 (ja) | 2014-08-18 | 2019-03-06 | オリンパス株式会社 | 培養容器、多光子励起顕微鏡、及び観察方法 |
JP5731704B1 (ja) | 2014-09-05 | 2015-06-10 | 日本写真印刷株式会社 | 培養容器 |
US10625264B2 (en) * | 2015-02-27 | 2020-04-21 | Corning Incorporated | Fitted lid for multi-well plate |
BR112017018259B1 (pt) * | 2015-02-27 | 2023-11-28 | Mastaplex Limited | Método para realizar um teste de suscetibilidade antimicrobiana e kit de teste |
CN204874491U (zh) * | 2015-07-17 | 2015-12-16 | 杭州秀川科技有限公司 | 一种细菌培养板 |
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- 2016-12-14 JP JP2018556108A patent/JP6832954B2/ja active Active
- 2016-12-14 EP EP16924151.0A patent/EP3556844B1/en active Active
- 2016-12-14 WO PCT/JP2016/087301 patent/WO2018109886A1/ja unknown
- 2016-12-14 US US16/464,830 patent/US11618873B2/en active Active
- 2016-12-14 CN CN201680091212.2A patent/CN110023480B/zh active Active
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Publication number | Publication date |
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EP3556844A4 (en) | 2020-07-22 |
CN110023480A (zh) | 2019-07-16 |
WO2018109886A1 (ja) | 2018-06-21 |
CN110023480B (zh) | 2022-11-11 |
US20190292505A1 (en) | 2019-09-26 |
EP3556844B1 (en) | 2023-11-01 |
JPWO2018109886A1 (ja) | 2019-10-24 |
US11618873B2 (en) | 2023-04-04 |
EP3556844A1 (en) | 2019-10-23 |
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