JP6732422B2 - Culture room and culture facility - Google Patents

Culture room and culture facility Download PDF

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JP6732422B2
JP6732422B2 JP2015193307A JP2015193307A JP6732422B2 JP 6732422 B2 JP6732422 B2 JP 6732422B2 JP 2015193307 A JP2015193307 A JP 2015193307A JP 2015193307 A JP2015193307 A JP 2015193307A JP 6732422 B2 JP6732422 B2 JP 6732422B2
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憲隆 福永
憲隆 福永
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization

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Description

本発明は、培養室および培養施設に関する。 The present invention relates to a culture room and a culture facility.

従来、ヒト等の動物の細胞を人工的に培養する場合、培養器（インキュベーター）を備える培養室内で培養されている（例えば、特許文献１参照）。 Conventionally, when artificially culturing cells of animals such as humans, the cells have been cultured in a culture chamber equipped with an incubator (see, for example, Patent Document 1).

ところで、妊娠を望む人が避妊なしで１〜２年以内に妊娠にいたることができない場合、不妊症と診断される。不妊症に対する治療（以下、不妊治療と称する）の１つに、体外受精（コンベンショナル体外受精、顕微授精）がある。体外受精では、受精卵を数日間（通常、２〜５日程度）培養した胚を、子宮に戻す。通常、体外受精の施術は、受精卵を培養する培養室を有する病院で行われる。 By the way, when a person who wants to become pregnant cannot reach pregnancy within 1 to 2 years without contraception, it is diagnosed as infertility. In vitro fertilization (conventional in vitro fertilization, microinsemination) is one of the treatments for infertility (hereinafter referred to as infertility treatment). In in vitro fertilization, embryos obtained by culturing fertilized eggs for several days (usually about 2 to 5 days) are returned to the uterus. Usually, in vitro fertilization is performed in a hospital having a culture room for culturing fertilized eggs.

従来、培養室には、空調機が設けられ、所定の温度、湿度に調整されていた。培養室は密閉されており、室内温度のむらが発生しやすいため、空調機の風量を大きくして培養室内の空気を循環させることによって、室内温度の均一化を図っていた。例えば、培養室の天井に比較的大きな空調機を２つ設けて、空調機から風量を大きくして清浄空気を培養室内に供給し、培養室内の空気を空調機が吸い込むことによって、培養室内の空気を循環させていた。 Conventionally, an air conditioner is provided in the culture room and adjusted to a predetermined temperature and humidity. Since the culture chamber is hermetically sealed and the temperature inside the culture chamber is likely to be uneven, the air temperature in the culture chamber is increased to circulate the air in the culture chamber to make the room temperature uniform. For example, by providing two relatively large air conditioners on the ceiling of the culture room, increasing the air volume from the air conditioner to supply clean air into the culture room, and sucking the air in the culture room into the culture room, It was circulating air.

特許第４８０３１９６号公報Japanese Patent No. 4803196

上述のような構成の培養室では、空調機から供給された清浄空気による風が強く循環するため、室内温度のむらの発生は抑制されるものの、培養室内の埃が舞う可能性があった。胚培養は、本来、女性の体内（卵管内）で行われることであるため、胚培養は、卵管内の環境に近い環境で行われることが好ましい。すなわち、培養室には、卵管内に近い、高い清浄度が要求される。特に、培養器等の胚培養に関する作業を行う機器の周辺における清浄度を高めたいという要望がある。 In the culture room having the above-described structure, the wind of the clean air supplied from the air conditioner circulates strongly, so that the generation of the uneven temperature in the room is suppressed, but the dust in the culture room may fly. Since embryo culture is originally performed in the female body (in the fallopian tube), embryo culture is preferably performed in an environment close to the environment in the fallopian tube. That is, the culture room is required to have high cleanliness close to that in the oviduct. In particular, there is a demand to increase the cleanliness around the devices such as incubators that perform work related to embryo culture.

本発明は、上述の課題の少なくとも一部を解決するためになされたものであり、以下の形態として実現することが可能である。 The present invention has been made to solve at least a part of the problems described above, and can be realized as the following modes.

（１）本発明の一形態によれば、人の受精卵を培養する培養器を内部に備える培養室が提供される。この培養室は、天井と、前記天井に設けられており、温度、湿度、および清浄度に調整された清浄空気を、前記培養室内に供給する複数の清浄空気供給口と、床と、前記床に設けられており、前記培養室内の空気が吸い出される吸気口であって、前記培養器の下に配置されている第１の吸気口と、を備え、前記複数の清浄空気供給口は、前記培養器の直上を避けて配置されている。 (1) According to one aspect of the present invention, there is provided a culture chamber including a culture vessel for culturing human fertilized eggs. This culture chamber is provided on the ceiling and the ceiling, and a plurality of clean air supply ports for supplying clean air adjusted to temperature, humidity and cleanliness into the culture chamber, a floor, and the floor. And a first intake port arranged below the incubator, which is an intake port for sucking out air in the culture chamber, and the plurality of clean air supply ports, It is arranged so as not to be directly above the incubator.

この形態の培養室によれば、複数の清浄空気供給口を備えるため、１個の清浄空気供給口から供給される清浄空気の量を抑えることができる、清浄空気供給口から供給される清浄空気の風により埃が舞上がるのを抑制することができる。また、複数の清浄空気供給口が培養器の直上を避けて配置されており、培養器に清浄空気による風が直接当たらないため、培養器付近に埃が舞上がるのを抑制することができる。さらに、第１の吸気口が培養器の下に配置されているため、培養器の下に溜まりやすい埃を効率よく培養室外へ排出させることができる。その結果、培養室の清浄度を向上させることができる。とりわけ、培養器近傍の清浄度を向上させることができる。 According to the culture chamber of this aspect, since the plurality of clean air supply ports are provided, the amount of clean air supplied from one clean air supply port can be suppressed, and the clean air supplied from the clean air supply port. It is possible to prevent dust from flying up due to the wind. Further, since the plurality of clean air supply ports are arranged so as not to be located directly above the incubator and the wind caused by the clean air does not directly impinge on the incubator, it is possible to prevent dust from flying around the incubator. Further, since the first intake port is arranged below the incubator, dust that easily accumulates under the incubator can be efficiently discharged to the outside of the incubator. As a result, the cleanliness of the culture chamber can be improved. In particular, the cleanliness in the vicinity of the incubator can be improved.

（２）上記形態培養室であって、前記床に設けられており、前記培養室内の空気が吸い出される吸気口であって、培養に関する作業を行う作業者が留まって作業するべき仮想作業領域に配置されている第２の吸気口を備え、前記複数の清浄空気供給口は、前記仮想作業領域の直上を避けて設けられてもよい。培養室内では、顕微鏡による受精卵の観察、顕微授精、培地の調製等、培養に関する作業を、人（作業者）が行う。このとき、作業者は、必要な時間、顕微鏡の前等に留まって作業を行う。作業者には埃が付着している可能性があり、また作業者の周囲には埃が漂っている可能性がある。この形態の培養室では、作業者が留まって作業するべき仮想作業領域に第２の吸気口が設けられているため、作業者が持ち込む埃を効率良く培養室外に排出することができる。また、清浄空気供給口が仮想作業領域の直上を避けて配置されているため、作業者に付着している埃や作業者の周囲に漂う埃を舞い上げるのを抑制することができる。 (2) The above-mentioned morphological culture chamber, which is provided on the floor, is an intake port through which air in the culture chamber is sucked out, and is a virtual work area in which an operator who performs the work relating to the culture should stay and work. The plurality of clean air supply ports may be provided so as not to be directly above the virtual work area. In the culturing chamber, a person (worker) performs work related to culturing, such as observing fertilized eggs with a microscope, microfertilization, and medium preparation. At this time, the worker works by staying in front of the microscope for a necessary time. The worker may be dusty, and there may be dust around the worker. In the culture room of this form, since the second intake port is provided in the virtual work area where the worker should stay and work, the dust brought in by the worker can be efficiently discharged to the outside of the culture room. Further, since the clean air supply port is arranged so as not to be located directly above the virtual work area, it is possible to prevent dust adhering to the worker or dust floating around the worker from flying up.

（３）上記形態培養室であって、内部に顕微鏡を備え、前記複数の清浄空気供給口は、前記顕微鏡の直上を避けて配置されてもよい。このようにすると、顕微鏡近傍の清浄度も向上させることができる。 (3) In the above-mentioned morphological culture chamber, a microscope may be provided inside, and the plurality of clean air supply ports may be arranged avoiding directly above the microscope. By doing so, the cleanliness in the vicinity of the microscope can be improved.

（４）上記形態培養室であって、前記培養に関する作業は、前記顕微鏡を用いた作業および培地の調製の少なくともいずれか一方でもよい。このようにすると、これらの作業が行われる領域の清浄度を向上させることができる。 (4) In the above-mentioned morphological culture room, the work relating to the culture may be at least one of the work using the microscope and the preparation of the medium. In this way, the cleanliness of the area where these operations are performed can be improved.

（５）本発明の他の形態によれば、培養施設が提供される。この培養施設は、人の***を処理する***処理室と、受精卵の培養に関する事務を行うための事務室と、の少なくともいずれか一方と、上記（１）から（４）のいずれか一項に記載の培養室と、を備える。この培養施設によれば、清浄度の高い培養室を備える培養施設を提供することができる。 (5) According to another aspect of the present invention, a culture facility is provided. This culturing facility is provided with at least one of a sperm processing room for processing human sperm and an office for carrying out affairs regarding culturing of fertilized eggs, and any one of the above (1) to (4). The culture chamber according to 1. According to this culture facility, it is possible to provide a culture facility including a culture chamber with high cleanliness.

上述した本発明の各形態の有する複数の構成要素はすべてが必須のものではなく、上述の課題の一部又は全部を解決するため、あるいは、本明細書に記載された効果の一部又は全部を達成するために、適宜、前記複数の構成要素の一部の構成要素について、その変更、削除、新たな他の構成要素との差し替え、限定内容の一部削除を行うことが可能である。また、上述の課題の一部又は全部を解決するため、あるいは、本明細書に記載された効果の一部又は全部を達成するために、上述した本発明の一形態に含まれる技術的特徴の一部又は全部を上述した本発明の他の形態に含まれる技術的特徴の一部又は全部と組み合わせて、本発明の独立した一形態とすることも可能である。 All of the plurality of constituent elements of each embodiment of the present invention described above are not indispensable, and in order to solve a part or all of the above problems, or a part or all of the effects described in the present specification. In order to achieve the above, it is possible to appropriately change or delete some of the constituent elements of the plurality of constituent elements, replace them with new constituent elements, and partially delete the limited contents. Further, in order to solve some or all of the above problems, or to achieve some or all of the effects described in the present specification, the technical features included in one embodiment of the present invention described above. Part or all of the technical features included in the other forms of the present invention described above may be combined with each other to form an independent form of the present invention.

本発明の一実施形態としての培養室を側面から模式的に示す説明図である。It is an explanatory view showing the culture room as one embodiment of the present invention typically from the side. 培養室における清浄空気供給口および吸気口の配置を説明するための説明図である。It is explanatory drawing for demonstrating arrangement|positioning of a clean air supply port and an intake port in a culture room.

Ａ．第１実施形態：
図１は、本発明の一実施形態としての培養室１００を側面から模式的に示す説明図である。図２は、培養室１００における清浄空気供給口７０および吸気口８０の配置を説明するための説明図である。培養室１００は、人の受精卵を培養する胚培養を行う部屋であり、体外受精を行う培養施設１０００を備える病院内に設けられている。図２に示すように、培養施設１０００は、培養室１００と、***処理室２００と、事務室３００と、を備える。***処理室２００は、人の***を処理する室である。具体的には、患者から採取された***の***検査、人工授精または体外受精の***処理を行う。事務室３００は、胚培養に関する事務を行う室である。培養室１００は、後述するように、高い清浄度が保たれるように構成されている。培養室１００の清浄度を保つには、培養室１００には極力物を持ち込まないことが好ましいため、胚培養に関する事務（データ処理等）は、事務室３００にて行われる。なお、胚培養士等の作業者Ｅは、培養室１００と事務室３００とを頻繁に行き来するため、事務室３００内は、培養室１００と同程度の清浄度が保たれるように構成されている。 A. First embodiment:
FIG. 1 is an explanatory view schematically showing a culture chamber 100 as one embodiment of the present invention from the side. FIG. 2 is an explanatory diagram for explaining the arrangement of the clean air supply port 70 and the intake port 80 in the culture chamber 100. The culture room 100 is a room for carrying out embryo culture for culturing human fertilized eggs, and is provided in a hospital equipped with a culture facility 1000 for in vitro fertilization. As shown in FIG. 2, the culture facility 1000 includes a culture room 100, a sperm processing room 200, and an office room 300. The sperm processing room 200 is a room for processing human sperm. Specifically, semen inspection of semen collected from a patient, sperm treatment for artificial insemination or in vitro fertilization is performed. The office 300 is a room for carrying out office work concerning embryo culture. The culture chamber 100 is configured to maintain high cleanliness, as will be described later. In order to maintain the cleanliness of the culture room 100, it is preferable to bring as few objects as possible into the culture room 100, so office work (data processing etc.) relating to embryo culture is performed in the office room 300. Since the worker E such as an embryo culture person frequently moves back and forth between the culture room 100 and the office room 300, the inside of the office room 300 is configured so as to be kept as clean as the culture room 100. ing.

培養室１００内には、培養器（インキュベーター）６０、顕微鏡５０等が備えられている。図１では、培養室１００内の設備の一例として、顕微鏡５０と培養器６０を図示し、顕微鏡５０の操作を行う作業者Ｅ（例えば、胚培養士）を共に図示している。培養室１００は、天井２０と、清浄空気供給口７０と、床１０と、吸気口８０と、を備える。 The culture chamber 100 is equipped with a culture vessel (incubator) 60, a microscope 50, and the like. In FIG. 1, a microscope 50 and an incubator 60 are illustrated as an example of equipment in the culture room 100, and an operator E (for example, an embryo cultivator) who operates the microscope 50 is also illustrated. The culture chamber 100 includes a ceiling 20, a clean air supply port 70, the floor 10, and an intake port 80.

培養室１００の外には、清浄空気生成部９３が設けられている。清浄空気生成部９３は、所定の温度、所定の湿度、および所定の清浄度に調整された清浄空気を生成する。清浄空気生成部９３は、ダクト９２を介して床下室９１と接続され、ダクト９６を介して清浄空気供給口７０と接続されている。清浄空気生成部９３は、メッシュ状の吸気口８０を介して培養室１００内の空気を吸引し、所定の温度、所定の湿度に調整して、フィルターによって濾過された清浄空気をダクト９６を介して清浄空気供給口７０から培養室１００内に供給する。なお、清浄空気生成部９３は、濾過された外気を取込むことにより、培養室１００内を陽圧にしている。培養室１００の壁には、複数個のダンパー２２が設けられ（図１では１つを例示している）、培養室１００内の圧力が調整されている。後述するように、培養室１００は複数の清浄空気供給口７０を備え、ダクト９６は分岐されて、全ての清浄空気供給口７０と接続されている。なお、吸気口８０はパンチングメタルで構成されてもよい。 A clean air generating unit 93 is provided outside the culture chamber 100. The clean air generation unit 93 generates clean air adjusted to have a predetermined temperature, a predetermined humidity, and a predetermined cleanliness. The clean air generating unit 93 is connected to the underfloor chamber 91 via a duct 92, and is connected to the clean air supply port 70 via a duct 96. The clean air generating unit 93 sucks the air in the culture chamber 100 through the mesh-shaped intake port 80, adjusts the temperature to a predetermined temperature and a predetermined humidity, and passes the clean air filtered by the filter through the duct 96. And supply it into the culture chamber 100 through the clean air supply port 70. The clean air generating unit 93 keeps the culture chamber 100 at a positive pressure by taking in the filtered outside air. A plurality of dampers 22 are provided on the wall of the culture chamber 100 (one is illustrated in FIG. 1), and the pressure inside the culture chamber 100 is adjusted. As described later, the culture chamber 100 includes a plurality of clean air supply ports 70, and the duct 96 is branched and connected to all the clean air supply ports 70. The intake port 80 may be made of punching metal.

図２に示すように、培養室１００は、複数の清浄空気供給口７０と、複数の吸気口８０と、を備える。複数の吸気口８０は、第１の吸気口８１と、第２の吸気口８２と、第３の吸気口８３と、を備える。図２において、明瞭に示すために、清浄空気供給口７０，吸気口８０の一部に符号を付して、他の符号の記載を省略している。図２においてハッチングを付した箇所が、吸気口８０である。 As shown in FIG. 2, the culture chamber 100 includes a plurality of clean air supply ports 70 and a plurality of intake ports 80. The plurality of intake ports 80 include a first intake port 81, a second intake port 82, and a third intake port 83. In FIG. 2, for the sake of clarity, reference numerals are attached to parts of the clean air supply port 70 and the intake port 80, and other reference symbols are omitted. The hatched portion in FIG. 2 is the intake port 80.

培養室１００内には、２台の培養器（インキュベーター）６０と、６台の顕微鏡５０と、培地の調製が行われる作業台３０（培養室１００内の紙面左端に配置されている）が備えられている。そして、複数の作業者Ｅ（例えば、胚培養士）が、培地の調製、顕微鏡５０を用いた受精卵の観察、顕微授精等、胚培養に関する作業を行っている。培養室１００が備える機器等およびその台数は本実施形態に限定されず、適宜変更可能である。但し、培養室１００は、少なくとも１台の培養器を備える。本実施形態では、作業者Ｅが留まって作業するのは、顕微鏡５０の前と、作業台３０の前である。胚培養は、培養器６０の中で行われ、作業者Ｅは、受精卵が入った容器を培養器６０に対して出し入れするものの、培養器６０の前に留まって、そこで観察等の作業を行うことはない。本実施形態において、作業者Ｅが留まって作業する場所を、仮想作業領域ＷＳと称し、図２に破線で囲んで示す。なお、仮想作業領域ＷＳは、実際に作業者Ｅが留まっているか否かに関わらず、作業者Ｅが留まって作業すると想定される領域（本実施形態では、顕微鏡５０の前と、作業台３０の前）をいう。なお、本実施形態では、培養器６０の前に留まって作業することは想定されていないが、培養器６０の前に留まって作業する状態が想定される場合には、培養器６０の前も仮想作業領域ＷＳに含まれる。ここで、留まって作業するとは、座って作業する、立って作業する等を含む概念である。 In the culture chamber 100, there are provided two incubators 60, six microscopes 50, and a workbench 30 in which the medium is prepared (which is arranged at the left end of the paper in the culture chamber 100). Has been. Then, a plurality of workers E (for example, an embryo cultivator) perform operations related to embryo culture, such as preparation of a medium, observation of a fertilized egg using the microscope 50, and microinsemination. The devices and the like included in the culture chamber 100 and the number thereof are not limited to the present embodiment, and can be changed as appropriate. However, the culture chamber 100 includes at least one incubator. In this embodiment, the worker E stays and works in front of the microscope 50 and in front of the work table 30. The embryo culture is carried out in the incubator 60, and the worker E puts the container containing the fertilized egg in and out of the incubator 60, but stays in front of the incubator 60 and performs operations such as observation there. There is nothing to do. In the present embodiment, a place where the worker E stays and works is referred to as a virtual work area WS, which is surrounded by a broken line in FIG. The virtual work area WS is an area where the worker E is supposed to stay and work regardless of whether or not the worker E actually stays (in the present embodiment, in front of the microscope 50 and the work table 30). Before). In addition, in the present embodiment, it is not assumed that the incubator 60 stays in front of the incubator 60 to work, but when it is assumed that the incubator 60 stays in front of the incubator 60 to perform the work, It is included in the virtual work area WS. Here, staying and working is a concept including sitting and working, standing and working.

清浄空気供給口７０は、１０個設けられている。そして、清浄空気供給口７０は、培養器６０、顕微鏡５０、作業台３０、および仮想作業領域ＷＳの直上を避けて配置されている。本明細書中において、「避けて配置」とは、床１０に平行投影された清浄空気供給口７０が、床１０に平行投影された培養器６０，顕微鏡５０，作業台３０、および仮想作業領域ＷＳと、略重ならないように配置されていることをいう。ここで、「略重ならない」とは、床１０に平行投影された清浄空気供給口７０の一部が、床１０に平行投影された培養器６０，顕微鏡５０，作業台３０，または仮想作業領域ＷＳに重なる場合であって、その重なる部分の面積が、床１０に平行投影された清浄空気供給口７０の面積の１０％未満の場合を含む概念である。 Ten clean air supply ports 70 are provided. The clean air supply port 70 is arranged so as not to be located directly above the incubator 60, the microscope 50, the workbench 30, and the virtual work area WS. In the present specification, "avoiding" means that the clean air supply port 70 projected in parallel on the floor 10 has the incubator 60, the microscope 50, the work table 30, and the virtual work area projected in parallel on the floor 10. It is arranged so as not to substantially overlap with WS. Here, "not substantially overlapping" means that a part of the clean air supply port 70 projected in parallel on the floor 10 is projected in parallel on the floor 10, the incubator 60, the microscope 50, the work table 30, or the virtual work area. This is a concept including a case where the area overlaps with WS and the area of the overlapping area is less than 10% of the area of the clean air supply port 70 that is projected in parallel on the floor 10.

培養室１００の天井２０の面積は、約６５．１ｍ２であり、清浄空気供給口７０の面積は、約０．６７ｍ２である。培養室１００では、天井２０の面積に対して、非常に小さい面積の清浄空気供給口７０を、胚培養に関する作業が行われる場所の直上を避けて、複数配置されている。清浄空気供給口７０の面積は、天井２０の面積の０．５〜３％程度が好ましい。 The area of the ceiling 20 of the culture room 100 is about 65.1 m 2 , and the area of the clean air supply port 70 is about 0.67 m 2 . In the culture room 100, a plurality of clean air supply ports 70 each having a very small area with respect to the area of the ceiling 20 are arranged so as not to be located directly above the place where the work relating to embryo culture is performed. The area of the clean air supply port 70 is preferably about 0.5 to 3% of the area of the ceiling 20.

培養室１００は、床１０に２１個の吸気口８０を備える。上述の通り、吸気口８０は、第１の吸気口８１，第２の吸気口８２，および第３の吸気口８３を含む。第１の吸気口８１は、培養器６０の下に配置されている。図２に示す例では、２台の培養器６０の下に、それぞれ１つずつ、第１の吸気口８１が配置されている。図２に示す例では、第１の吸気口８１の全体が培養器６０の下になるように、配置されているが、第１の吸気口８１の少なくとも一部が培養器６０の下になるように配置されればよい。但し、第１の吸気口８１において、培養器６０の下になる面積が大きいほど、集塵効率が向上されるため、好ましい。例えば、第１の吸気口８１の面積の５０％以上が培養器６０の下になるように、第１の吸気口８１が配置されるのが好ましい。第１の吸気口８１の面積の８０％以上が培養器６０の下になるように、第１の吸気口８１が配置されると、さらに、好ましい。 The culture room 100 includes 21 intake ports 80 on the floor 10. As described above, the intake port 80 includes the first intake port 81, the second intake port 82, and the third intake port 83. The first intake port 81 is arranged below the incubator 60. In the example shown in FIG. 2, one first intake port 81 is arranged under each of the two incubators 60. In the example shown in FIG. 2, the first intake port 81 is arranged under the incubator 60, but at least a part of the first intake port 81 is under the incubator 60. It may be arranged as follows. However, in the first intake port 81, the larger the area under the incubator 60, the better the dust collection efficiency, which is preferable. For example, it is preferable that the first intake port 81 is arranged such that 50% or more of the area of the first intake port 81 is below the incubator 60. It is further preferable that the first intake port 81 is arranged such that 80% or more of the area of the first intake port 81 is below the incubator 60.

第２の吸気口８２は、作業者Ｅが胚培養に関する作業を行うことが想定される仮想作業領域ＷＳに配置されている。本実施形態では、顕微鏡５０を用いた作業が行われる仮想作業領域ＷＳと、培地の調製が行われる仮想作業領域ＷＳに配置されている。図２に示すように、第２の吸気口８２は、６台の顕微鏡５０を用いて作業（例えば、受精卵の観察，顕微授精等）を行う作業者Ｅの下に配置されている。また、第２の吸気口８２は、培養室１００内の紙面左端に配置された作業台３０に向かって座る作業者Ｅ（例えば、培地の調製を行う作業者）の下に配置されている。作業者Ｅが仮想作業領域ＷＳで作業するべく座った場合に、床１０に平行投影された作業者Ｅの少なくとも一部が第２の吸気口８２と重なるように、第２の吸気口８２が配置されている。床１０に平行投影された作業者Ｅが第２の吸気口８２と重なる面積が、床１０に平行投影された作業者Ｅの面積に対する割合が大きいほど、集塵効率が向上されるため、好ましい。例えば、床１０に平行投影された作業者Ｅが第２の吸気口８２と重なる面積が、床１０に平行投影された作業者Ｅの面積の５０％以上になるように、第２の吸気口８２が配置されるのが好ましい。床１０に平行投影された作業者Ｅが第２の吸気口８２と重なる面積が、床１０に平行投影された作業者Ｅの面積の８０％以上になるように、第２の吸気口８２が配置されると、さらに、好ましい。床１０に平行投影された作業者Ｅの全体が、第２の吸気口８２に含まれるように、第２の吸気口８２が配置されるのが、最も好ましい。 The second intake port 82 is arranged in a virtual work area WS where the worker E is supposed to perform a work related to embryo culture. In the present embodiment, they are arranged in a virtual work area WS in which a work using the microscope 50 is performed and a virtual work area WS in which a medium is prepared. As shown in FIG. 2, the second intake port 82 is arranged under the worker E who performs work (for example, observation of fertilized eggs, microinsemination, etc.) using the six microscopes 50. Further, the second intake port 82 is arranged below the worker E (for example, a worker who prepares the medium) sitting toward the work table 30 arranged at the left end of the drawing in the culture chamber 100. When the worker E sits down to work in the virtual work area WS, the second intake port 82 is arranged so that at least a part of the worker E projected in parallel on the floor 10 overlaps with the second intake port 82. It is arranged. It is preferable that the area in which the worker E projected in parallel on the floor 10 and the second intake port 82 overlap with the area of the worker E projected in parallel on the floor 10 is higher because the dust collection efficiency is improved. .. For example, the second intake port is arranged so that the area of the worker E projected in parallel on the floor 10 and the second intake port 82 is 50% or more of the area of the worker E projected in parallel on the floor 10. 82 is preferably arranged. The second intake port 82 is arranged so that the area of the worker E projected on the floor 10 in parallel with the second intake port 82 is 80% or more of the area of the worker E projected on the floor 10 in parallel. When arranged, it is further preferable. Most preferably, the second intake port 82 is arranged such that the entire worker E projected in parallel on the floor 10 is included in the second intake port 82.

第３の吸気口８３は、培養器６０および作業領域に関係なく、培養室１００の床１０にバランスよく散在する。 The third air inlets 83 are scattered in a well-balanced manner on the floor 10 of the culture chamber 100 regardless of the incubator 60 and the work area.

本実施形態の培養室１００は、密閉され、高い清浄度および防音レベルを有している。清浄度は、例えば、ＩＳＯ（国際標準化機構）基準によるクラス７、アメリカ航空宇宙局規格(通称、ＮＡＳＡ規格)によるクラス１０，０００〜１００，０００程度である。防音レベルは、例えば、５５〜６５ｄＢ（Ａ）である。なお、培養室１００は、エアシャワー室（不図示）を介して入室可能に構成されている。 The culture chamber 100 of the present embodiment is hermetically sealed and has high cleanliness and soundproof level. The cleanliness is, for example, Class 7 according to the ISO (International Organization for Standardization) standard, and Class 10,000 to 100,000 according to the United States Aerospace Standard (commonly known as NASA standard). The soundproof level is, for example, 55 to 65 dB(A). The culture chamber 100 is configured to be enterable via an air shower chamber (not shown).

本実施形態の培養室１００によれば、複数の清浄空気供給口７０と複数の吸気口８０を備え、清浄空気供給口７０が天井全体に散在し、吸気口８０が床全体に散在するため、空気の流れが、培養室１００の全体に亘り、天井２０から床１０への一方向になる。そのため、培養室１００内の温度のむらが発生しにくく、温度管理を容易にすることができる。 According to the culture chamber 100 of the present embodiment, the plurality of clean air supply ports 70 and the plurality of intake ports 80 are provided, the clean air supply ports 70 are scattered over the entire ceiling, and the intake ports 80 are scattered over the entire floor. The air flow is in one direction from the ceiling 20 to the floor 10 throughout the culture chamber 100. Therefore, the temperature in the culture chamber 100 is less likely to be uneven, and the temperature control can be facilitated.

また、複数の清浄空気供給口７０を設けることにより、１個の清浄空気供給口７０から供給される清浄空気の量を抑えることができるため、清浄空気供給口７０から供給される清浄空気の風により埃が舞上がるのを抑制することができる。 Further, since the amount of clean air supplied from one clean air supply port 70 can be suppressed by providing the plurality of clean air supply ports 70, the wind of clean air supplied from the clean air supply port 70 can be reduced. Thus, it is possible to prevent dust from flying up.

さらに、複数の清浄空気供給口７０が、培養器６０，顕微鏡５０，作業台３０の直上を避けて配置されているため、清浄空気供給口７０から供給される清浄空気による風は、培養器６０，顕微鏡５０，作業台３０を避けて天井側から床に向かって流れる。すなわち、清浄空気供給口７０から供給される清浄空気による風がそれらに直接当たらない。そのため、清浄空気供給口７０からの風により培養器６０，顕微鏡５０，作業台３０付近に埃が舞うことを抑制することができる。 Further, since the plurality of clean air supply ports 70 are arranged avoiding the positions directly above the incubator 60, the microscope 50, and the work table 30, the wind from the clean air supplied from the clean air supply port 70 is incubator 60. , Avoiding the microscope 50 and the workbench 30, flows from the ceiling side toward the floor. That is, the wind from the clean air supplied from the clean air supply port 70 does not directly hit them. Therefore, it is possible to prevent dust from flying around the incubator 60, the microscope 50, and the workbench 30 due to the wind from the clean air supply port 70.

一般的に、培養器６０は、その底面と床１０との距離が小さく、培養器６０の下には埃が溜まりやすい。培養室１００では、第１の吸気口８１が培養器６０の下に配置されているため、培養器６０の下の埃を効率よく培養室１００外へ排出させることができる。 In general, the distance between the bottom surface of the incubator 60 and the floor 10 is small, and dust is likely to accumulate under the incubator 60. In the culture chamber 100, since the first intake port 81 is arranged below the incubator 60, the dust under the incubator 60 can be efficiently discharged to the outside of the culture chamber 100.

また、顕微鏡５０の前、培地の調製を行う作業台３０の前には、作業者Ｅが留まって作業を行う。作業者Ｅには埃が付着している可能性があり、また作業者Ｅの周囲に埃が浮遊している可能性がある。培養室１００では、第２の吸気口８２は、上述の仮想作業領域ＷＳに配置されており、作業者Ｅが椅子に座る等留まった場合に、作業者Ｅの下に第２の吸気口８２が在る。そのため、胚培養に関する作業を行っている作業者Ｅが持ち込んだ埃を、効率よく培養室１００外へ排出させることができる。 In addition, an operator E stays in front of the microscope 50 and in front of the workbench 30 for preparing the medium to perform the work. Dust may be attached to the worker E, and dust may be floating around the worker E. In the culture chamber 100, the second intake port 82 is arranged in the above-described virtual work area WS, and when the worker E stays on a chair or the like, the second intake port 82 is located below the worker E. There is. Therefore, the dust brought in by the worker E who is working on the embryo culture can be efficiently discharged to the outside of the culture chamber 100.

以上説明したように、本実施形態の培養室１００によれば、複数の清浄空気供給口７０が、胚培養に関する作業が行われる領域を避けて配置されると共に、複数の吸気口８０が、培養器６０の下、および胚培養に関する作業が行われる領域に配置されているため、集塵効率が向上され、胚培養に関する作業が行われる場所の清浄度が向上される。 As described above, according to the culture chamber 100 of the present embodiment, the plurality of clean air supply ports 70 are arranged while avoiding the region where the work related to embryo culture is performed, and the plurality of intake ports 80 are used for the culture. Since it is placed under the container 60 and in the area where the work relating to the embryo culture is performed, the dust collection efficiency is improved and the cleanliness of the place where the work relating to the embryo culture is performed is improved.

Ｂ．変形例：
なお、この発明は上記の実施形態に限られるものではなく、その要旨を逸脱しない範囲において種々の態様において実施することが可能であり、例えば次のような変形も可能である。 B. Modification:
The present invention is not limited to the above-described embodiment, and can be implemented in various modes without departing from the scope of the invention, and the following modifications are possible, for example.

（１） 清浄空気供給口７０および吸気口８０の個数は、上記実施形態に限定されず、培養室１００の広さ（床面積、天井面積）、培養器６０を含む胚培養に関する機器の個数等に応じて適宜変更可能である。また、清浄空気供給口７０および吸気口８０の配置は上記実施形態に限定されない。清浄空気供給口７０は、少なくとも、培養器６０の直上を避けて配置されればよい。さらに、顕微鏡５０の直上を避けて配置されると好ましい。吸気口８０は、少なくとも培養器６０の下に配置されればよい。 (1) The numbers of the clean air supply port 70 and the intake port 80 are not limited to those in the above embodiment, and the size of the culture chamber 100 (floor area, ceiling area), the number of devices for embryo culture including the incubator 60, etc. It can be changed as appropriate. Further, the arrangement of the clean air supply port 70 and the intake port 80 is not limited to the above embodiment. It suffices that the clean air supply port 70 is arranged so as to avoid at least directly above the incubator 60. Further, it is preferable that the microscope 50 is arranged so as not to be directly above the microscope 50. The intake port 80 may be disposed at least below the incubator 60.

（２） 上記実施形態において、第２の吸気口８２は、顕微鏡５０を用いた作業が行われる仮想作業領域ＷＳおよび培地の調製が行われる仮想作業領域ＷＳの両方に配置されているが、これに限定されない。例えば、顕微鏡５０を用いた作業が行われる仮想作業領域ＷＳおよび培地の調製が行われる仮想作業領域ＷＳのいずいれか一方に配置されてもよい。また、上記実施形態では、顕微鏡５０を用いた作業が行われる仮想作業領域ＷＳの全てに対応して第２の吸気口８２が配置されているが、６台の顕微鏡５０のうち少なくとも１台の顕微鏡５０に対する仮想作業領域ＷＳに対応して１つの第２の吸気口８２が配置されればよい。 (2) In the above embodiment, the second intake port 82 is arranged in both the virtual work area WS in which the work using the microscope 50 is performed and the virtual work area WS in which the medium is prepared. Not limited to. For example, it may be arranged in either one of the virtual work area WS in which the work using the microscope 50 is performed and the virtual work area WS in which the medium is prepared. Further, in the above-described embodiment, the second intake port 82 is arranged corresponding to all the virtual work areas WS where the work using the microscope 50 is performed, but at least one of the six microscopes 50 is provided. It suffices that one second intake port 82 is arranged corresponding to the virtual work area WS for the microscope 50.

（３） 上記実施形態において、培養施設１０００は、培養室１００，***処理室２００，および事務室３００を備えるが、***処理室２００および事務室３００のいずれか一方を備えない構成にしてもよい。 (3) In the above embodiment, the culture facility 1000 includes the culture room 100, the sperm processing room 200, and the office 300, but may have a configuration that does not include any one of the sperm processing room 200 and the office 300. ..

本発明は、上述の実施形態や実施例、変形例に限られるものではなく、その趣旨を逸脱しない範囲において種々の構成で実現することができる。例えば、発明の概要の欄に記載した各形態中の技術的特徴に対応する実施形態、実施例、変形例中の技術的特徴は、上述の課題の一部又は全部を解決するために、あるいは、上述の効果の一部又は全部を達成するために、適宜、差し替えや、組み合わせを行うことが可能である。また、その技術的特徴が本明細書中に必須なものとして説明されていなければ、適宜、削除することが可能である。 The present invention is not limited to the above-described embodiments, examples, and modified examples, and can be realized with various configurations without departing from the spirit thereof. For example, the technical features in the embodiments, examples, and modifications corresponding to the technical features in each mode described in the column of the outline of the invention are to solve some or all of the above problems, or In order to achieve some or all of the above-mentioned effects, it is possible to appropriately replace or combine them. If the technical features are not described as essential in this specification, they can be deleted as appropriate.

１０…床
２０…天井
２２…ダンパー
３０…作業台
５０…顕微鏡
６０…培養器
７０…清浄空気供給口
８０…吸気口
８１…第１の吸気口
８２…第２の吸気口
８３…第３の吸気口
９１…床下室
９２…ダクト
９３…清浄空気生成部
９６…ダクト
１００…培養室
２００…***処理室
３００…事務室
１０００…培養施設
Ｅ…作業者
ＷＳ…仮想作業領域 10... Floor 20... Ceiling 22... Damper 30... Workbench 50... Microscope 60... Incubator 70... Clean air supply port 80... Intake port 81... First intake port 82... Second intake port 83... Third intake air Mouth 91...Underfloor 92...Duct 93...Clean air generating unit 96...Duct 100...Culturing room 200...Sperm processing room 300...Office 1000...Culturing facility E...Worker WS...Virtual work area

Claims (5)

人の受精卵を培養するための培養室であって、
天井と、
前記天井に設けられており、温度、湿度、および清浄度が調整された清浄空気を、前記培養室内に供給する複数の清浄空気供給口と、
前記天井に向き合った床と、
前記床に設けられており、前記培養室内の空気が吸い出される複数の第１の吸気口と、
前記床の上に、前記床との間に空間を設けて設置された培養器と、
を備え、
前記複数の第１の吸気口のうちの少なくとも１つが、前記培養器の下に配置され、
前記複数の清浄空気供給口は、前記培養器の直上を避けて配置されており、
前記複数の清浄空気供給口は、培養に関する作業を行う作業者が留まって作業するべき仮想作業領域の直上を避けて配置されている、
培養室。 A culture room for culturing human fertilized eggs,
With the ceiling
A plurality of clean air supply ports that are provided on the ceiling and that supply clean air whose temperature, humidity, and cleanliness are adjusted into the culture chamber,
A floor facing the ceiling,
A plurality of first intake ports provided on the floor for sucking out air in the culture chamber;
On the floor, an incubator installed with a space between the floor,
Equipped with
At least one of the plurality of first inlets is located below the incubator,
The plurality of clean air supply ports are arranged avoiding directly above the incubator ,
The plurality of clean air supply ports are arranged so as to avoid a position directly above a virtual work area in which an operator performing a culture-related work should stay and work,
Culture room. 請求項１に記載の培養室であって、
前記床に設けられており、前記培養室内の空気が吸い出される吸気口であって、前記仮想作業領域に配置されている第２の吸気口を備え、
前記複数の清浄空気供給口は、前記仮想作業領域の直上を避けて配置されている、培養室。 The culture chamber according to claim 1, wherein
Is provided on the floor, a inlet air of the culture chamber is sucked, a second air inlet is arranged in the virtual working area,
A culture chamber in which the plurality of clean air supply ports are arranged so as not to be directly above the virtual work area. 請求項２に記載の培養室であって、
内部に顕微鏡を備え、
前記複数の清浄空気供給口は、前記顕微鏡の直上を避けて配置されている、培養室。 The culture chamber according to claim 2,
Equipped with a microscope inside,
A culture chamber in which the plurality of clean air supply ports are arranged so as not to be directly above the microscope. 請求項３に記載の培養室であって、
前記培養に関する作業は、前記顕微鏡を用いた作業および培地の調製の少なくともいずれか一方である、培養室。 The culture chamber according to claim 3,
The culture room, wherein the work related to the culture is at least one of work using the microscope and preparation of a medium. 培養施設であって、
人の***を処理する***処理室と、受精卵の培養に関する事務を行うための事務室と、の少なくともいずれか一方と、
請求項１から４のいずれか一項に記載の培養室と、
を備える、培養施設。 A culture facility,
At least one of a sperm processing room that processes human sperm and an office for performing office work related to culture of fertilized eggs,
A culture chamber according to any one of claims 1 to 4,
A culture facility equipped with.
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