JP6695319B2 - マルチプレックスpcrチップ及びそれを含むマルチプレックスpcr装置 - Google Patents
マルチプレックスpcrチップ及びそれを含むマルチプレックスpcr装置 Download PDFInfo
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Description
220 第2板
222 流入部
224 反応領域
226 流出部
230 第3板
240 プローブ
310 親水性物質
410 領域
510 プローブ固定部
512 中心部
514 周辺部
610 気泡除去部
710 気泡除去部
Claims (14)
- 相異なる複数の核酸分子を同時に検出するために、前記核酸分子の相異なる増幅された配列と特異的に混成化される多数の混成化反応用プローブ(probe)を含み、前記多数のプローブは前記核酸分子の相異なる増幅された配列と特異的に混成化された後に同一の蛍光染料によって標識されるものであり、前記多数のプローブは、お互いに離隔して固着され、前記多数のプローブ間の離隔位置に基づいて前記多数のプローブそれぞれと混成化する核酸分子の配列を識別できることを特徴とするマルチプレックスPCR(Multiplex Polymerase Chain Reaction)チップであって、
前記多数のプローブが、前記マルチプレックスPCRチップのPCR反応領域の上部内面に下方に向けて突設される突設部の下面に配置されて、下方へ垂れた形態であり、また、前記多数のプローブが、多孔構造を形成する接着物質を介して、前記突設部の下面に結合され、さらに、前記突設部は前記反応領域内の気泡が前記プローブ上に位置することを防止するためのものであることを特徴とするマルチプレックスPCRチップ。 - 前記多孔構造は、ハイドロゲル(hydrogel)、アガロース(agarose)及びパラフィン(paraffin)のうちから選ばれる少なくとも1種からなることを特徴とする請求項1に記載のマルチプレックスPCRチップ。
- 前記マルチプレックスPCRチップは、平板状の第1板と;前記第1板上に配置され、流入部、反応領域及び流出部を有する第2板と;前記第2板上に配置されて前記反応領域をカバーし、下部の表面に前記プローブが離隔配置される第3板と;を含むことを特徴とする請求項1に記載のマルチプレックスPCRチップ。
- 前記マルチプレックスPCRチップは、前記プローブが配置される中心部と、前記中心部を囲むように前記中心部に隣接して突設される周辺部と、からなるプローブ固定部をさらに含むことを特徴とする請求項3に記載のマルチプレックスPCRチップ。
- 前記マルチプレックスPCRチップは、前記反応領域内の気泡が前記プローブ上に位置することを防止するために、前記第3板の下部内面から下方に向けて突設される光透過性材質の気泡除去部を含むことを特徴とする請求項3に記載のマルチプレックスPCRチップ。
- 前記第1板は、ポリメチルメタクリレート(polymethylmethacrylate、PMMA)、ポリカーボネート(polycarbonate、PC)、シクロオレフィンコポリマー(cycloolefin c opolymer、COC)、ポリアミド(polyamide、PA)、ポリエチレン(polyethylene、
PE)、ポリプロピレン(polypropylene、PP)、ポリフェニレンエーテル(polypheny lene ether、PPE)、ポリスチレン(polystyrene、PS)、ポリオキシメチレン(pol yoxymethylene、POM)、ポリエーテルエーテルケトン(polyetheretherketone、PEEK)、ポリテトラフルオロエチレン(polytetrafluoroethylene、PTFE)、ポリ塩化ビニル(polyvinylchloride、PVC)、ポリフッ化ビニリデン(polyvinylidene fluo ride、PVDF)、ポリブチレンテレフタレート(polybutyleneterephthalate、PBT)、フッ素化エチレンプロピレン(fluorinated ethylenepropylene、FEP)、パーフルオロアルコキシアルカン(perfluoralkoxyalkane、PFA)、及びその組み合わせからなる群から選択される熱可塑性樹脂又は熱硬化性樹脂材質であり、
前記第2板及び第3板のそれぞれは、ポリジメチルシロキサン(polydimethylsiloxane 、PDMS)、シクロオレフィンコポリマー(cycle olefin copolymer、COC)、ポリメチルメタクリレート(polymethylmetharcylate、PMMA) 、ポリカーボネート(polycarbonate、PC)、ポリプロピレンカーボネート(polypropylene carbonate、PPC)、ポリエーテルスルホン(polyether sulfone、PES)、ポリエチレンテレフタレート(polyethylene terephthalate、PET)、及びその組み合わせからなる群から選択される材質からなることを特徴とする請求項3に記載のマルチプレックスPCRチップ。 - 前記第2板の前記反応領域に対応するように前記第3板の下面の少なくとも一部領域が突設されることを特徴とする請求項3に記載のマルチプレックスPCRチップ。
- 前記流入部及び前記流出部のそれぞれは、流体が流入される開口部と、前記開口部に隣接して突設される突出部と、を含み、前記突出部は、熱を印加されて融解されることにより前記開口部を密封することを特徴とする請求項3に記載のマルチプレックスPCRチップ。
- 請求項1に係るマルチプレックスPCRチップと;
前記マルチプレックスPCRチップ内の前記プローブに向かって励起光線(excitation light)を照射する光提供部と;
前記励起光線によって多数のプローブから発生する蛍光(emission light)を検出する光検出部と;を含み、
前記光提供部及び前記光検出部による検出は、単一波長の光を用いて行われることを特徴とするマルチプレックスPCR装置。 - 請求項1に係るマルチプレックスPCRチップと;
前記マルチプレックスPCRチップに接触し、前記マルチプレックスPCRチップにマルチプレックスPCRのための熱を伝達する少なくとも1つの熱ブロックと;を含むことを特徴とするマルチプレックスPCR装置。 - 前記マルチプレックスPCRチップが装着されるチップホルダーと;前記チップホルダーを熱ブロック上で(above)/上へ(to)左右及び/又は上下に移動させる駆動部と;をさらに含み、
前記熱ブロックは、第1熱ブロック及び第2熱ブロックを含むことを特徴とする請求項10に記載のマルチプレックスPCR装置。 - 前記第1熱ブロック及び前記第2熱ブロックのうちいずれか一方の熱ブロックは、PCR反応の変性ステップの温度を維持するように具現され、他方の熱ブロックは、PCR反応のアニーリング及び伸長(または増幅)ステップの温度を維持するように具現されたこ とを特徴とする請求項11に記載のマルチプレックスPCR装置。
- 前記第1熱ブロックと前記第2熱ブロックは、相互に熱交換が起こらないように離隔配置されることを特徴とする請求項11に記載のマルチプレックスPCR装置。
- 前記駆動部は、左右方向に延設されたレールと;レールに沿って左右方向にスライド移動可能に配置され、上下方向にスライド移動可能な接続部材と;を含み、接続部材の一端にはチップホルダーが配置されることを特徴とする請求項11に記載のマルチプレックスPCR装置。
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KR1020140093312A KR101696259B1 (ko) | 2014-07-23 | 2014-07-23 | 멀티플렉스 pcr 칩 및 이를 포함하는 멀티플렉스 pcr 장치 |
KR10-2014-0093312 | 2014-07-23 | ||
PCT/KR2015/006322 WO2016013770A1 (ko) | 2014-07-23 | 2015-06-22 | 멀티플렉스 pcr 칩 및 이를 포함하는 멀티플렉스 pcr 장치 |
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KR101972579B1 (ko) * | 2016-05-25 | 2019-08-23 | (주)옵토레인 | 피씨알모듈 및 이를 이용한 검사방법 |
KR101885087B1 (ko) * | 2017-06-12 | 2018-08-03 | 티엔에스(주) | 랩온어칩 기반 분자진단칩 |
CN107058063B (zh) * | 2017-06-12 | 2019-08-20 | 博奥生物集团有限公司 | 一种基于微流控芯片的用于多重核酸扩增产物荧光检测的方法 |
GB201716961D0 (en) * | 2017-10-16 | 2017-11-29 | Quantumdx Group Ltd | Microfluidic devices with bubble diversion |
KR102246609B1 (ko) * | 2018-08-01 | 2021-04-30 | 주식회사 미코바이오메드 | 복수의 열 블록을 구비한 핵산 증폭 장치 |
KR102272251B1 (ko) | 2019-05-02 | 2021-07-02 | 재단법인 오송첨단의료산업진흥재단 | 다중 분석을 위한 분기형 바이오 칩 |
TWI715997B (zh) * | 2019-06-13 | 2021-01-11 | 新加坡商克雷多生物醫學私人有限公司 | 一種可即時偵測一種以上螢光訊號之聚合酶鏈式反應裝置 |
FR3098827A1 (fr) | 2019-07-17 | 2021-01-22 | Commissariat à l'Energie Atomique et aux Energies Alternatives | Dispositif micro-fluidique incluant de réaction d'amplification |
KR102478830B1 (ko) * | 2020-07-14 | 2022-12-20 | 주식회사 미루시스템즈 | 서로 다른 온도범위로 구획화된 복수의 챔버를 포함하는 pcr 장치 |
KR102365010B1 (ko) * | 2020-07-30 | 2022-02-21 | 주식회사 진시스템 | 멀티플렉스 pcr 칩 및 이를 이용한 멀티플렉스 pcr 방법 |
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EP3173469A4 (en) | 2018-01-24 |
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