JP6623401B2 - 飼料原料及びその用途 - Google Patents
飼料原料及びその用途 Download PDFInfo
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- JP6623401B2 JP6623401B2 JP2015076671A JP2015076671A JP6623401B2 JP 6623401 B2 JP6623401 B2 JP 6623401B2 JP 2015076671 A JP2015076671 A JP 2015076671A JP 2015076671 A JP2015076671 A JP 2015076671A JP 6623401 B2 JP6623401 B2 JP 6623401B2
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- feed
- acid
- mass
- rapeseed
- rumen
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Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Description
〔実施例1〜2、比較例1〜2〕菜種由来リン脂質のin vitro試験(1)
in vitroのウシルーメン液を用いた発酵試験において、菜種リン脂質が添加されたときの、短鎖脂肪酸の生成に及ぼす影響を調べた。比較のため、菜種由来の脂質(菜種油)が添加されたときの発酵試験も行なった。
粗飼料としてイタリアンライグラス(乾草)と濃厚飼料として市販濃厚飼料(製品名:そよ風の薫り、日本配合飼料株式会社製)とを質量基準で1:1にて混合することにより飼料Aを得た。以下、粗飼料と濃厚飼料との比率(質量基準)を、粗濃比ということがある。市販濃厚飼料の表示を表1に示す。
菜種油製造時の脱ガム工程で副生したガム質を、減圧乾燥することにより、クルード菜種レシチンを得た。このクルード菜種レシチンは、アセトン可溶物35〜40%を含む粘稠なペースト状であった。このクルード菜種レシチンを5倍量のアセトンに溶解し、静置分離にて下層を回収し、再び5倍量のアセトンに溶解した。この操作を5回繰り返した。得られた下層部を減圧乾燥することにより、アセトン可溶物が1.0質量%になるまで脱油された菜種レシチンを得た。リン脂質含量は60.2質量%だった。また、この菜種レシチンは、1.0質量%の水分を有し、粉末状であった。脱油菜種レシチンの組成を、表2に示す。
ルーメンフィステルを装着したF1ウシ(月齢40ヶ月)をルーメン液発酵試験に用いた。このウシを、コンクリート敷の牛房へ個体ごとに収容した。ウシに飼料Aを、1日当たり8kg(濃厚飼料4kg/日)、3週間、給与した。なお、試験3週間前及び試験中は、ウシに抗菌剤、その他薬剤を投与しなかった。
飼料Aの3週間給与の翌朝(給餌前)に、ウシからルーメン液を約500mL採取した。採取したルーメン液500mLを4重ガーゼで濾過した。この濾液とMcDougall’s Bufferとを5:4の割合で混合することにより、ルーメン濾液を希釈した。得られたルーメン希釈液9mLを15mL容バイアル瓶に分注した。
〔GC条件〕
Injection temp.:250℃
Column:Nukol(Supelco Inc.),30m×0.25mm×0.25μm
Initial temp.:100℃
Program rate:15℃/min
Final temp.:185℃
Detector:FID(250℃)
Carrier flow rate:1.0mL/min
Carrier gas:He
発酵基質を繊維質からデンプンに変更した発酵試験を実施した。具体的には、実施例1、並びに比較例1及び2において、発酵基質として100mgのコーンスターチを用いた以外は、前記実施例等と同様の方法で、発酵試験を実施した。結果を表4に示す。
菜種レシチンを含む飼料を動物へ給餌したときの短鎖脂肪酸の産生に及ぼす影響を調べるため、in vivoでのウシルーメン液を用いる発酵試験を実施した。比較のため、菜種油が添加されたときの発酵試験も行なった。
上記飼料Aに、実施例1に記載の菜種レシチンを、リン脂質質量基準で0.271質量%添加することにより、飼料Bを得た。
上記粗飼料と上記濃厚飼料とを粗濃比1:4にて混合することにより、飼料Cを得た。
上記飼料Cに、実施例1に記載の菜種レシチンを、リン脂質質量基準で0.433質量%添加することにより、飼料Dを得た。
Claims (9)
- 菜種由来のリン脂質を30〜60.2質量%含む飼料原料をリン脂質の質量基準で0.271〜0.433質量%、そして粗飼料を20質量%以上かつ60質量%以下含む反芻動物用飼料。
- 反芻動物の消化管での短鎖脂肪酸産生を向上させることを特徴とする、請求項1に記載の反芻動物用飼料。
- 前記短鎖脂肪酸産生の向上は、酢酸、プロピオン酸、イソ酪酸、酪酸、イソ吉草酸及び吉草酸の総量の増大である、請求項2に記載の反芻動物用飼料。
- 前記飼料原料が、アセトン可溶物含量が4質量%以下の脱油菜種レシチンを含む、請求項1〜3のいずれかに記載の反芻動物用飼料。
- 菜種由来のリン脂質を30〜60.2質量%含む飼料原料をリン脂質の質量基準で0.271〜0.433質量%、そして粗飼料を20質量%以上かつ60質量%以下含む反芻動物用飼料を、リン脂質の質量基準で8〜400g/日、反芻動物に給与することを含む、反芻動物の飼育方法。
- 反芻動物の消化管での短鎖脂肪酸の産生を向上させることを特徴とする、請求項5に記載の反芻動物の飼育方法。
- 前記短鎖脂肪酸産生の向上は、酢酸、プロピオン酸、イソ酪酸、酪酸、イソ吉草酸及び吉草酸の総量の増大である、請求項6に記載の反芻動物の飼育方法。
- 前記飼料原料が、アセトン可溶物含量が4質量%以下の脱油菜種レシチンを含む、請求項5〜7のいずれかに記載の反芻動物の飼育方法。
- 菜種由来のリン脂質を30〜60.2質量%含む飼料原料をリン脂質の質量基準で0.271〜0.433質量%、そして粗飼料を20質量%以上かつ60質量%以下配合することを含む、反芻動物用飼料の製造方法。
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