JP6568362B2 - 物体長を判定するための光の空間的変調 - Google Patents
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- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
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- G01P—MEASURING LINEAR OR ANGULAR SPEED, ACCELERATION, DECELERATION, OR SHOCK; INDICATING PRESENCE, ABSENCE, OR DIRECTION, OF MOVEMENT
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Description
Claims (8)
- 複数のマスク特徴を有する空間フィルタと、
流れ方向に沿って流路内を移動している少なくとも1つの物体から放射された光を検知するように配置された少なくとも1つの検出器であり、検知された光の強度がマスク特徴に応じて時間変調され、検知された光に応答してパルスのシーケンスを含む時間的に変化する電気信号を生成するように構成される検出器と、
パルスの振幅極値の割合でパルスの少なくとも一部のパルス幅を測定し、測定されたパルス幅に基づいて、流れ方向に沿った物体の長さを判定するように構成されている分析器とを備え、
分析器が、
各点がp i =(x i ,y i )によって与えられるマスク特徴測定点{p i }のセットをフィッティングする関数f({p i },x)を判定するように構成されており、各x i はi番目のマスク特徴の長さと関連しており、各y i はi番目のマスク特徴と関連しているi番目の測定されたパルス幅と関連しており、関数がマスク特徴の測定点{p i }のセットに基づいて、長さxの仮定されたマスク特徴の期待パルス幅測定値を予測する、システム。 - マスク特徴が第1の特徴及び第2の特徴を含み、第1の特徴が実質的に透明であり、第2の特徴が実質的に不透明である、請求項1に記載のシステム。
- マスク特徴が、第1の線形チャープ又は対数チャープパターンに配置された第1の部分と、第2の線形又は対数チャープパターンアルゴリズムに配置された第2の部分とを含み、第1のパターン及び第2のパターンが、空間フィルタを横方向に横切って延在する中心線まわりに対称であり、
第1のマスク特徴が実質的に透明であり、第2のマスク特徴が実質的に不透明であり、透過性特徴が中心線に近接した第1の特定長さを有し、
第1のパターンの透明特徴が、流れ方向に沿った第2の特定値の長さの線形減少を有し、第2のパターンの透明特徴が、流れ方向に沿った第2の特定値の長さの線形増加を有する、請求項1に記載のシステム。 - マスク特徴が第1の特徴及び第2の特徴を含み、第1の特徴の長さが空間フィルタの流れ方向に沿って一定であり、第2の特徴の長さが空間フィルタの流れ方向に沿って一定であり、
分析器が、
正方向パルスのパルス幅を測定し、
負方向パルスのパルス幅を測定し、
パルス対の幅の平均値に基づいて物体の長さを判定するように構成されており、
各パルス対が正方向パルス及び隣接する負方向パルスを含む、請求項1に記載のシステム。 - 分析器が、さらに、パルス幅対の平均の傾きに基づいて、物体の速度が増加又は減少しているかどうかを判定するように構成されている、請求項4に記載のシステム。
- マスク特徴が第1の特徴及び第2の特徴を含み、
第1の特徴の長さが空間フィルタの流れ方向に沿って一定であり、
第2の特徴の長さが空間フィルタの流れ方向に沿って一定であり、
少なくとも1つの物体が複数の物体を含み、
検出器が、流れ方向に沿って流路内を移動している複数の物体から放射された光を検知するように配置されており、
分析器が、
正方向パルスのパルス幅を測定し、
負方向パルスのパルス幅を測定し、
正方向パルス及び負方向パルスのパルス幅に基づいて流路に沿ってともに移動している複数の物体の数を識別するように構成されている、請求項1に記載のシステム。 - 空間フィルタの流れ方向に沿って流路内を移動している少なくとも1つの物体から放射された光を検知することであり、空間フィルタが、流れ方向に沿って第2の特徴と交互になっている第1の特徴を含む複数のマスク特徴を有し、第1の特徴が第1の光透過特性を有し、第2の特徴が第1の光透過特性とは異なる第2の光透過特性を有し、検知された光の強度が、マスク特徴に応じて時間変調されることと、
検知された光に応答して複数の時間変調されたパルスを含む時間的に変化する電気信号を生成することと、
パルスの最大極値の割合でパルスのパルス幅を測定することと、
測定されたパルス幅に基づいて、流れ方向に沿った物体の長さを判定することとを備え、
物体の長さを判定することが、
各点がp i =(x i ,y i )によって与えられるマスク特徴測定点{p i }のセットをフィッティングする関数f({p i },x)を判定することであり、各x i はi番目のマスク特徴の長さと関連しており、各y i はi番目のマスク特徴と関連しているi番目の測定されたパルス幅と関連しており、関数がマスク特徴の測定点{p i }に基づいて、長さxの仮定されたマスク特徴の期待パルス幅測定値を予測することと、
関数を使用して物体の長さを外挿することとを備える、方法。 - 複数のマスク特徴を有する空間フィルタと、
流れ方向に沿って流路内を移動している少なくとも1つの物体から放射された光を検知するように配置された少なくとも1つの検出器であり、検知された光の強度がマスク特徴に応じて時間変調され、検知された光に応答してパルスのシーケンスを含む時間的に変化する電気信号を生成するように構成される検出器と、
パルスの振幅極値の10%から40%又は60%から90%の範囲でパルスの少なくとも一部のパルス幅を測定し、パルス幅に基づいて、物体の流れ方向に沿った長さを判定するように構成されている分析器とを備え、
分析器が、
各点がp i =(x i ,y i )によって与えられるマスク特徴測定点{p i }のセットをフィッティングする関数f({p i },x)を判定するように構成されており、各x i はi番目のマスク特徴の長さと関連しており、各y i はi番目のマスク特徴と関連しているi番目の測定されたパルス幅と関連しており、関数がマスク特徴の測定点{p i }のセットに基づいて、長さxの仮定されたマスク特徴の期待パルス幅測定値を予測する、システム。
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US14/181,530 US9952033B2 (en) | 2014-02-14 | 2014-02-14 | Spatial modulation of light to determine object length |
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