JP6533104B2 - 細胞工学用支持体の製造方法 - Google Patents
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- 239000011148 porous material Substances 0.000 claims description 63
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- 239000000843 powder Substances 0.000 claims description 16
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- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 8
- 229940038773 trisodium citrate Drugs 0.000 description 8
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- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
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- 229960000448 lactic acid Drugs 0.000 description 4
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- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
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- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Natural products OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
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- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
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- 239000011780 sodium chloride Substances 0.000 description 2
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- 235000019263 trisodium citrate Nutrition 0.000 description 2
- 229920000954 Polyglycolide Polymers 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
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- 238000007561 laser diffraction method Methods 0.000 description 1
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- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L67/00—Compositions of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Compositions of derivatives of such polymers
- C08L67/04—Polyesters derived from hydroxycarboxylic acids, e.g. lactones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
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Description
前記骨格構造体の表面に形成された乳酸−グリコール酸共重合体を含むコート層と、を有する細胞工学用支持体の製造方法であって、
ポリ−D−乳酸、またはポリ−L−乳酸を含む顆粒状の多孔質体である第1の顆粒状多孔質物質と、乳酸−グリコール酸共重合体を含む顆粒状の多孔質体である第2の顆粒状多孔質物質とを混合して混合体を調製する混合工程と、
前記混合体を型に入れ加圧・加熱する加圧・加熱工程と、を有し、
前記加圧・加熱工程では、前記乳酸−グリコール酸共重合体の融点以上、前記第1の顆粒状多孔質物質に含まれるポリ−D−乳酸、またはポリ−L−乳酸の融点未満まで加熱する細胞工学用支持体の製造方法を提供する。
[第1の実施形態]
本実施形態では細胞工学用支持体の製造方法の一構成例について説明する。
(混合工程)
混合工程においては、ポリ−D−乳酸、またはポリ−L−乳酸を含み、顆粒状の多孔質体である第1の顆粒状多孔質物質と、乳酸−グリコール酸共重合体を含み、顆粒状の多孔質体である第2の顆粒状多孔質物質とを混合して混合体を調製できる。
(加圧・加熱工程)
次に、混合体を型に入れ加圧・加熱する加圧・加熱工程について説明する。
[第2の実施形態]
次に、本実施形態の細胞工学用支持体の製造方法の他の構成例について説明する。
(骨格構造体準備工程)
まず、骨格構造体準備工程について説明する。骨格構造体準備工程では、ポリ−D−乳酸、またはポリ−L−乳酸を含む骨格構造体を準備することができる。
60℃以上とすることで、ポリ−D−乳酸、またはポリ−L−乳酸同士の結合を十分に高めることができ、形状安定性を特に高めることができる。また、200℃以下とすることで、ポリ−D−乳酸、またはポリ−L−乳酸が変性することを確実に防ぐことができる。
(熱処理工程)
次に熱処理工程について説明する。熱処理工程では、骨格構造体の表面に乳酸−グリコ−ル酸共重合体の粉末を分散した後、熱処理を行うことができる。
[実施例1]
以下の手順により細胞工学用支持体を作製した。
(混合工程)
まず、以下の手順により、ポリ−L−乳酸からなり、顆粒状の多孔質体である第1の顆粒状多孔質物質を準備した。
(加圧・加熱工程)
得られた混合体を内径9mm×高さ10mmのガラス容器内に高さが約7mmとなるように入れた。そして、直径9mmのチタン棒で1500g/cm2で加圧した状態の体積を保って50℃で20分間加熱して、直径9mm×高さ約7mmの円柱形状をした細胞工学用支持体が得られた。
[実施例2]
第1の顆粒状多孔質物質として、ポリ−D−乳酸からなる顆粒状の多孔質体を用いた点以外は、実施例1と同様にして細胞工学用支持体を作製し、評価を行った。
[実施例3]
以下の手順により細胞工学用支持体を作製した。
(骨格構造体準備工程)
まず、以下の手順により、ポリ−L−乳酸を含有する骨格構造体を準備する骨格構造体準備工程を実施した。
(熱処理工程)
次に得られた骨格構造体を熱処理するための加熱炉内に配置し、該骨格構造体の表面に、平均粒径が200μmの乳酸−グリコ−ル酸共重合体粉末を分散した。なおこの際、骨格構造体と、分散する乳酸−グリコール酸共重合体粉末との質量の合計のうち、分散する乳酸−グリコール酸共重合体粉末の割合が20質量%となるように、乳酸−グリコール酸共重合体粉末を分散した。
[実施例4]
ポリ−D−乳酸を含有する骨格構造体を用いた点以外は実施例3と同様にして、細胞工学用支持体を作製し、評価を行った。
11 骨格構造体
12 コート層
Claims (2)
- ポリ−D−乳酸、またはポリ−L−乳酸を含む骨格構造体と、
前記骨格構造体の表面に形成された乳酸−グリコール酸共重合体を含むコート層と、を有する細胞工学用支持体の製造方法であって、
ポリ−D−乳酸、またはポリ−L−乳酸を含む、顆粒状の多孔質体である第1の顆粒状多孔質物質と、乳酸−グリコール酸共重合体を含む、顆粒状の多孔質体である第2の顆粒状多孔質物質とを混合して混合体を調製する混合工程と、
前記混合体を型に入れ加圧・加熱する加圧・加熱工程と、を有し、
前記加圧・加熱工程では、前記乳酸−グリコール酸共重合体の融点以上、前記第1の顆粒状多孔質物質に含まれるポリ−D−乳酸、またはポリ−L−乳酸の融点未満まで加熱する細胞工学用支持体の製造方法。 - ポリ−D−乳酸、またはポリ−L−乳酸を含む骨格構造体と、
前記骨格構造体の表面に形成された乳酸−グリコール酸共重合体を含むコート層と、を有する細胞工学用支持体の製造方法であって、
ポリ−D−乳酸、またはポリ−L−乳酸を含む、顆粒状の多孔質体を型に入れ、500g/cm 2 以上3000g/cm 2 以下、60℃以上200℃以下で、加圧・加熱し、ポリ−D−乳酸、またはポリ−L−乳酸を含む前記骨格構造体を準備する骨格構造体準備工程と、
前記骨格構造体の表面に乳酸−グリコ−ル酸共重合体の粉末を分散した後、熱処理を行う熱処理工程と、を有し、
前記熱処理工程では、前記乳酸−グリコール酸共重合体の融点以上、前記骨格構造体に含まれるポリ−D−乳酸、またはポリ−L−乳酸の融点未満まで加熱する細胞工学用支持体の製造方法。
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JP2015123277A JP6533104B2 (ja) | 2015-06-18 | 2015-06-18 | 細胞工学用支持体の製造方法 |
US15/168,360 US9795710B2 (en) | 2015-06-18 | 2016-05-31 | Method of fabricating scaffold for tissue engineering |
EP16001272.0A EP3106184B1 (en) | 2015-06-18 | 2016-06-06 | Method of fabricating scaffold for tissue engineering |
US15/708,535 US20180000988A1 (en) | 2015-06-18 | 2017-09-19 | Method of fabricating scaffold for tissue engineering |
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JPH01104635A (ja) * | 1987-10-16 | 1989-04-21 | Taki Chem Co Ltd | 多孔質体の製造方法 |
DE4003233A1 (de) * | 1990-02-03 | 1991-08-08 | Boehringer Ingelheim Kg | Verwendung von polymeren aus glykol- und milchsaeure mit mehrwertigen alkoholen als beschichtungsmaterial fuer chirurgisches nahtmaterial |
US20050271701A1 (en) | 2000-03-15 | 2005-12-08 | Orbus Medical Technologies, Inc. | Progenitor endothelial cell capturing with a drug eluting implantable medical device |
US20050031598A1 (en) * | 2002-12-10 | 2005-02-10 | Shulamit Levenberg | Engineering three-dimensional tissue structures using differentiating embryonic stem cells |
US20060083771A1 (en) | 2004-10-15 | 2006-04-20 | Gc Corporation | Block-shaped scaffold for tissue engineering and production method thereof |
JP4738800B2 (ja) * | 2004-10-15 | 2011-08-03 | 株式会社ジーシー | ブロック状細胞工学用支持体の製造方法 |
US8029575B2 (en) * | 2005-10-25 | 2011-10-04 | Globus Medical, Inc. | Porous and nonporous materials for tissue grafting and repair |
US9446172B2 (en) | 2011-05-10 | 2016-09-20 | Abbott Cardiovascular Systems Inc. | Modification of bioabsorbable stent to reduce thrombogenecity |
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2015
- 2015-06-18 JP JP2015123277A patent/JP6533104B2/ja active Active
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2016
- 2016-05-31 US US15/168,360 patent/US9795710B2/en active Active
- 2016-06-06 EP EP16001272.0A patent/EP3106184B1/en active Active
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2017
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Publication number | Publication date |
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EP3106184B1 (en) | 2018-09-12 |
JP2017006029A (ja) | 2017-01-12 |
US20180000988A1 (en) | 2018-01-04 |
US9795710B2 (en) | 2017-10-24 |
EP3106184A1 (en) | 2016-12-21 |
US20160367721A1 (en) | 2016-12-22 |
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