JP6334841B2 - ソルガム発酵及び培養方法、ソルガム発酵エキスの製造方法並びにソルガム発酵エキス配合物の製造方法 - Google Patents
ソルガム発酵及び培養方法、ソルガム発酵エキスの製造方法並びにソルガム発酵エキス配合物の製造方法 Download PDFInfo
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- JP6334841B2 JP6334841B2 JP2012134318A JP2012134318A JP6334841B2 JP 6334841 B2 JP6334841 B2 JP 6334841B2 JP 2012134318 A JP2012134318 A JP 2012134318A JP 2012134318 A JP2012134318 A JP 2012134318A JP 6334841 B2 JP6334841 B2 JP 6334841B2
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- 235000011684 Sorghum saccharatum Nutrition 0.000 title claims description 29
- 239000000284 extract Substances 0.000 title claims description 12
- 238000004519 manufacturing process Methods 0.000 title claims description 12
- 239000000203 mixture Substances 0.000 title claims description 7
- 238000000855 fermentation Methods 0.000 title claims description 6
- 230000004151 fermentation Effects 0.000 title claims description 6
- 238000012136 culture method Methods 0.000 title claims 4
- 241000209072 Sorghum Species 0.000 title description 6
- 240000006394 Sorghum bicolor Species 0.000 claims description 23
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 9
- 241000588912 Pantoea agglomerans Species 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
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- 241000237502 Ostreidae Species 0.000 claims 1
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- 239000000463 material Substances 0.000 description 11
- 241000209140 Triticum Species 0.000 description 10
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- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 7
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- 235000013339 cereals Nutrition 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
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- 206010020751 Hypersensitivity Diseases 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
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- 229940041514 candida albicans extract Drugs 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
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- 241000588724 Escherichia coli Species 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 208000004262 Food Hypersensitivity Diseases 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
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- 239000007836 KH2PO4 Substances 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
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- 241000520272 Pantoea Species 0.000 description 1
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- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
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- 235000021329 brown rice Nutrition 0.000 description 1
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- 238000000605 extraction Methods 0.000 description 1
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- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
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- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
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Images
Landscapes
- Fodder In General (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
食塩、ポリペプトン、イーストエクストラクト(酵母抽出物)、グリセリン、塩化アンモニウム、リン酸第一カリウム、硫酸第一鉄、硫酸マグネシウム、硫酸アンモニウム、リン酸第二カリウム、塩化カルシウム、ホワイトソルガム、アミラーゼ、ヌクレイシン、エマゾールO-30V、KM-72、水酸化ナトリウム、硫酸、リン酸、チャコール(活性炭)、セルピュアS65(珪藻土濾過助剤)、酢酸、マックス1000
パントエア・アグロメランス(Pantoea agglomerans)
3L容量のジャーファーメンターに2Lの培地を入れて滅菌し、パントエア・アグロメランスの前培養液を5%加えて、30℃で、50時間培養した。培地組成は下記のとおりである。ホワイトソルガムは事前にアミラーゼで酵素処理を行って使用した。培養中、pHは6.7〜7に調整し、発泡する場合には、アンチフォームを添加した。
White Sorghum 0.5%
α-amylase 0.009%
Nucleicin 0.004%
Glycerol 6%
NH4Cl 1%
K2HPO4 0.1%
KH2PO4 0.03%
FeSO4・7H2O 0.005%
CaCl2・2H2O 0.01%
KM72 0.015%
MgSO4・7H2O 0.05%
上記培養終了後、90℃20分加熱して滅菌し、同時に糖脂質を抽出した。その後、雑菌の繁殖を防ぐため酢酸にてpHを酸性にし、遠心して菌体成分を除去した。
上記遠心後の上澄に活性炭を加え微細残渣を吸着させて遠心除去した。遠心後の上澄はさらにセライトを加えてろ過することで、清澄にした。清澄となった溶液は、UF膜を通して濃縮した。化粧品用とする場合には、UF膜濃縮溶液にNaClとEtOHを加えて糖脂質を一旦沈殿させ、沈殿物を滅菌蒸留水に再溶解して糖脂質濃度が0.01%になるように再調整し、「ホワイトソルガム発酵エキス」(Somacy-WS001)とした。
・ホワイトソルガム発酵エキス(Somacy-WS001)の1%水溶液
・ネガティブコントロールとして蒸留水を使用
・エントリーした人数 53人
・試験を終了した人数 51人
・年齢 22−64歳
・性別 男性 10人
女性 43人
・人種 白人 40人
スペイン人 10人
アジア人 3人
・パッチの種類:Parke-Davis Hypoallergenic Readi Bandagesまたは同等品
・1ml容量のシリンジ(針なし)
被験者は、試験施設にて背中にパッチ貼付けを行い、その後帰宅して24時間後に自分でパッチをはがす。およそ24時間後に試験施設に行き、皮膚の状態の観察を行うとともに、問題がなければ繰り返しのパッチ貼付けを行う。この手順を3週間にわたり、月、水、金曜日に行い、9回まで繰り返した(一次刺激試験)。パッチ貼付けは、0.2mlの試験物質とネガティブコントロールとしての蒸留水を閉塞用低アレルギー性のパッチにつけて、被験者の背中の肩甲骨下部分で、背中より左または右の皮膚に直接貼り付けた。9回の閉塞パッチテストの終了後、10日から14日たった時に、再度24時間の閉塞パッチを1回のみ行い、パッチをはがしてから24時間後と48時間後に皮膚の状態を観察した(感作性試験)。尚、パッチ試験中に、パッチを水に塗らしたり、直接日にさらした場合、あるいは指定日に試験施設に来なかった場合、その被験者の結果はデータから除外した。最終判定は皮膚科医が行った。
51人の被験者について、試験期間中、いずれの種類の有害事象も見られなかった。すなわち試験物質は、皮膚の閉塞パッチテストを行った結果、一次刺激はなく、また感作性もないことが示された。
Claims (5)
- ソルガム(和名:タカキビ)、グリセリン及び硫酸第一鉄を含む培地をパントエア・アグロメランスによって発酵させて同時に該パントエア・アグロメランスを培養することを特徴とする発酵及び培養方法。
- 前記ソルガムがソルガム、ホワイトソルガム又はこれらの混合物であることを特徴とする請求項1記載の発酵及び培養方法。
- 請求項1又は2記載の発酵及び培養方法でソルガムを発酵及び培養する工程を含むことを特徴とするソルガム発酵エキスの製造方法。
- 請求項3記載の方法で製造したソルガム発酵エキスを配合する工程を含むことを特徴とするソルガム発酵エキス配合物の製造方法。
- 前記ソルガム発酵エキス配合物が医薬品、動物用医薬品、医薬部外品、化粧品、食品、機能性食品、飼料、又は浴用剤であることを特徴とする請求項4記載のソルガム発酵エキス配合物の製造方法。
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