JP6079018B2 - Biological sample transfer device and biological sample transfer method - Google Patents

Biological sample transfer device and biological sample transfer method Download PDF

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JP6079018B2
JP6079018B2 JP2012155598A JP2012155598A JP6079018B2 JP 6079018 B2 JP6079018 B2 JP 6079018B2 JP 2012155598 A JP2012155598 A JP 2012155598A JP 2012155598 A JP2012155598 A JP 2012155598A JP 6079018 B2 JP6079018 B2 JP 6079018B2
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小百合 北嶋
小百合 北嶋
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    • A—HUMAN NECESSITIES
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    • A61B5/150992—Blood sampling from a fluid line external to a patient, such as a catheter line, combined with an infusion line; blood sampling from indwelling needle sets, e.g. sealable ports, luer couplings, valves
    • A—HUMAN NECESSITIES
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    • A61B5/15—Devices for taking samples of blood
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    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A61B5/150366—Blood collection bags, e.g. connected to the patient by a catheter comprising means for removing a small sample of collected blood from the bag
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above

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Description

本発明は、幹細胞や臍帯血等の細胞や血清等の生体試料を生体試料保存容器に移送する生体試料移送装置、及び該生体試料移送装置を用いた生体試料移送方法に関する。   The present invention relates to a biological sample transfer device for transferring a biological sample such as a cell such as stem cells or umbilical cord blood or serum to a biological sample storage container, and a biological sample transfer method using the biological sample transfer device.

従来、幹細胞や臍帯血等の細胞や血液から調製された血清といった生体試料は、生体から採取されてから使用されるまでの間、立体成形により形成された生体試料収容室を有する生体試料保存容器に収容された状態で凍結保存される（例えば、特許文献１参照）。より詳細には、生体から採取された生体試料は、まず、血液バッグ等の生体試料貯留容器に貯留される。次いで、生体試料貯留容器に貯留された生体試料は、この生体試料貯留容器に接続チューブを介して接続された生体試料保存容器に移送される。   Conventionally, a biological sample such as a stem cell or umbilical cord blood or a serum prepared from blood is collected from the living body until it is used, and the biological sample storage container has a biological sample storage chamber formed by three-dimensional molding. (See, for example, Patent Document 1). More specifically, a biological sample collected from a living body is first stored in a biological sample storage container such as a blood bag. Next, the biological sample stored in the biological sample storage container is transferred to a biological sample storage container connected to the biological sample storage container via a connection tube.

特開平１１−３４２１７９号公報JP-A-11-342179

生体試料貯留容器から生体試料保存容器に生体試料を移送する場合には、生体試料貯留容器を生体試料保存容器よりも上方に位置させることで生体試料貯留容器と生体試料保存容器との間に落差圧を生じさせ、この落差圧により生体試料を生体試料保存容器に移送する。
しかしながら、生体試料保存容器の生体試料収容室には、空気が存在するため、生体試料を生体試料保存容器に移送する場合には、生体試料収容室に存在する空気を生体試料と置換する作業が必要となる。 When transferring a biological sample from a biological sample storage container to a biological sample storage container, the biological sample storage container is positioned above the biological sample storage container so as to drop a gap between the biological sample storage container and the biological sample storage container. Pressure is generated, and the biological sample is transferred to the biological sample storage container by this drop pressure.
However, since air exists in the biological sample storage chamber of the biological sample storage container, when the biological sample is transferred to the biological sample storage container, the work of replacing the air present in the biological sample storage chamber with the biological sample is performed. Necessary.

ここで、生体試料保存容器に生体試料を保存する場合、生体試料の無菌状態を維持するために、生体試料保存容器、接続チューブ及び生体試料貯留容器の内部空間に外部の空気が侵入することを防ぐことが求められる。そのため、生体試料保存容器に生体試料を移送する場合には、生体試料保存容器を押しつぶして生体試料収容室に存在する空気を接続チューブを介して生体試料貯留容器側に移動させ、その後、押しつぶされた生体試料収容室に生体試料を移送するという作業を複数回繰り返すこととなる。   Here, when storing a biological sample in a biological sample storage container, in order to maintain the aseptic state of the biological sample, it is necessary that external air enters the internal space of the biological sample storage container, the connection tube, and the biological sample storage container. It is required to prevent. Therefore, when transferring a biological sample to the biological sample storage container, the biological sample storage container is crushed to move the air present in the biological sample storage chamber to the biological sample storage container side through the connection tube, and then crushed. The operation of transferring the biological sample to the biological sample storage chamber is repeated a plurality of times.

このように、生体試料と空気とを置換する作業を複数回繰り返した場合、生体試料と空気とが混合されることで生体試料に泡立ちが生じ、生体試料に悪影響が与えられてしまう。また、生体試料に泡立ちが生じることで、生体試料の一部が生体試料貯留容器に残存してしまい、生体試料を十分に生体試料保存容器に移送できなくなってしまう。   As described above, when the operation of replacing the biological sample and air is repeated a plurality of times, the biological sample and air are mixed to cause foaming, which adversely affects the biological sample. In addition, foaming of the biological sample causes a part of the biological sample to remain in the biological sample storage container, and the biological sample cannot be sufficiently transferred to the biological sample storage container.

従って、本発明は、生体試料を生体試料貯留容器から生体試料保存容器に移送する場合に、生体試料に悪影響を与えにくい生体試料移送装置及び生体試料移送方法を提供することを目的とする。   Therefore, an object of the present invention is to provide a biological sample transfer device and a biological sample transfer method that hardly cause adverse effects on a biological sample when the biological sample is transferred from the biological sample storage container to the biological sample storage container.

本発明は、生体試料が貯留される生体試料貯留容器と、可撓性を有する部材により構成され、立体成形により形成された生体試料収容室を有する生体試料保存容器と、前記生体試料貯留容器と前記生体試料保存容器とを無菌的かつ気密に接続する接続チューブと、前記接続チューブに設けられ、前記生体試料貯留容器、前記生体試料保存容器及び前記接続チューブの気密状態を維持しつつ、該接続チューブの内部の気体を外部に導出可能なポート部と、前記ポート部よりも前記生体試料貯留容器側に設けられる上流側閉止部と、を備える生体試料移送装置に関する。   The present invention provides a biological sample storage container in which a biological sample is stored, a biological sample storage container having a biological sample storage chamber that is formed by a three-dimensional molding and is configured by a flexible member, and the biological sample storage container. A connection tube for aseptically and airtightly connecting the biological sample storage container, and the connection tube, the connection tube while maintaining the airtight state of the biological sample storage container, the biological sample storage container and the connection tube The present invention relates to a biological sample transfer device including a port portion that can lead gas inside the tube to the outside, and an upstream closing portion that is provided closer to the biological sample storage container than the port portion.

また、前記上流側閉止部は、前記接続チューブにおける前記ポート部よりも前記生体試料貯留容器側に設けられ該接続チューブにおける流体の流通を遮断することが好ましい。   Further, it is preferable that the upstream closing portion is provided closer to the biological sample storage container than the port portion in the connection tube, and blocks the flow of fluid in the connection tube.

また、生体試料移送装置は、前記ポート部よりも前記生体試料保存容器側に設けられ前記接続チューブにおける流体の流通を遮断する下流側閉止部を更に備えることが好ましい。   Moreover, it is preferable that a biological sample transfer apparatus is further equipped with the downstream closing part provided in the said biological sample storage container side rather than the said port part, and interrupt | blocks the distribution | circulation of the fluid in the said connection tube.

また、前記ポート部は、膜状又は板状に形成された弾性部材と、該弾性部材に形成されたスリット部と、を有するセプタムを備えることが好ましい。   Moreover, it is preferable that the said port part is provided with the septum which has an elastic member formed in the film | membrane shape or plate shape, and the slit part formed in this elastic member.

また、前記ポート部は、前記接続チューブの内部から外部への気体の流通を許容し、外部から内部への気体の流通を遮断する一方弁を備えることが好ましい。   Moreover, it is preferable that the said port part is equipped with the one valve which accept | permits the flow of the gas from the inside of the said connection tube to the exterior, and interrupts | blocks the flow of the gas from the outside to the inside.

また、前記接続チューブは、前記生体試料貯留容器側に配置される上流側チューブと、前記生体試料保存容器側に配置される下流側チューブと、基端部が前記上流側チューブと前記下流側チューブとの接続部に接続される分岐チューブと、を備え、前記ポート部は、前記分岐チューブの先端部に接続されることが好ましい。   The connection tube includes an upstream tube disposed on the biological sample storage container side, a downstream tube disposed on the biological sample storage container side, and a proximal end portion of the upstream tube and the downstream tube. It is preferable that the port portion is connected to a distal end portion of the branch tube.

また、前記上流側閉止部及び前記下流側閉止部は、三方活栓により構成されることが好ましい。   Moreover, it is preferable that the said upstream side closing part and the said downstream side closing part are comprised by a three-way cock.

また、本発明は、可撓性を有する部材により構成され、立体成形により形成された生体試料収容室を有する生体試料保存容器と、一端部が前記生体試料保存容器に接続される接続チューブと、前記接続チューブの他端部に設けられ、該接続チューブと生体試料が収容された生体試料貯留容器とを連結可能な連結部材と、前記接続チューブに設けられ、該接続チューブの内部の気体を導出可能なポート部と、前記ポート部よりも前記連結部材側に設けられ前記接続チューブにおける流体の流通を遮断する上流側閉止部と、を備える生体試料移送装置に関する。   Further, the present invention is a biological sample storage container having a biological sample storage chamber formed of a flexible member and formed by three-dimensional molding, a connection tube having one end connected to the biological sample storage container, A connecting member provided at the other end of the connection tube and capable of connecting the connection tube and a biological sample storage container in which a biological sample is stored, and provided in the connection tube to derive gas inside the connection tube The present invention relates to a biological sample transfer device including a possible port portion and an upstream closing portion that is provided closer to the connecting member than the port portion and blocks the flow of fluid in the connection tube.

また、本発明は、上述のいずれかに記載の生体試料移送装置を用いた生体試料移送方法であって、前記上流側閉止部を閉止する第１閉止工程と、前記ポート部に吸引具を接続する吸引具接続工程と、前記吸引具により前記生体試料保存容器の内部の気体を吸引する吸引工程と、前記吸引具を前記ポート部から取り外す吸引具取り外し工程と、前記上流側閉止部を開放して前記生体試料貯留容器に収容された生体試料を前記生体試料保存容器に移送する生体試料移送工程と、を備える生体試料移送方法に関する。   The present invention is also a biological sample transfer method using the biological sample transfer device according to any one of the above, wherein a first closing step of closing the upstream closing portion and an aspirator connected to the port portion A suction tool connecting step, a suction step of sucking the gas inside the biological sample storage container by the suction tool, a suction tool removing step of removing the suction tool from the port part, and opening the upstream closing part. And a biological sample transfer step of transferring the biological sample stored in the biological sample storage container to the biological sample storage container.

また、本発明は、上述のいずれかに記載の生体試料移送装置を用いた生体試料移送方法であって、前記上流側閉止部を閉止する第１閉止工程と、前記ポート部に吸引具を接続する吸引具接続工程と、前記吸引具により前記生体試料保存容器の内部の気体を吸引する吸引工程と、前記下流側閉止部を閉止する第２閉止工程と、前記上流側閉止部を開放する第１開放工程と、前記吸引具に吸引された気体を前記生体試料貯留容器側に供給する気体供給工程と、前記吸引具を前記ポート部から取り外す吸引具取り外し工程と、前記下流側閉止部を開放して前記生体試料貯留容器に収容された生体試料を前記生体試料保存容器に移送する生体試料移送工程と、を備える生体試料移送方法に関する。   The present invention is also a biological sample transfer method using the biological sample transfer device according to any one of the above, wherein a first closing step of closing the upstream closing portion and an aspirator connected to the port portion A suction tool connecting step, a suction step of sucking the gas inside the biological sample storage container by the suction tool, a second closing step of closing the downstream closing portion, and a first opening of the upstream closing portion. 1 opening step, gas supplying step for supplying the gas sucked by the suction tool to the biological sample storage container side, suction tool removing step for removing the suction tool from the port part, and opening the downstream closing part And a biological sample transfer step of transferring a biological sample stored in the biological sample storage container to the biological sample storage container.

本発明によれば、生体試料を生体試料貯留容器から生体試料保存容器に導入する場合に、生体試料に悪影響を与えにくい生体試料移送装置及び生体試料移送方法を提供できる。   ADVANTAGE OF THE INVENTION According to this invention, when introduce | transducing a biological sample from a biological sample storage container to a biological sample storage container, the biological sample transfer apparatus and biological sample transfer method which do not have a bad influence on a biological sample can be provided.

本発明の第１実施形態に係る生体試料移送装置を示す図である。It is a figure which shows the biological sample transfer apparatus which concerns on 1st Embodiment of this invention. 第１実施形態に係る生体試料移送装置の生体試料保存容器を示す斜視図である。It is a perspective view which shows the biological sample storage container of the biological sample transfer apparatus which concerns on 1st Embodiment. 図２に示す生体試料保存容器の平面図である。It is a top view of the biological sample storage container shown in FIG. 図３のＡ−Ａ線断面図である。FIG. 4 is a sectional view taken along line AA in FIG. 3. 第１実施形態に係る生体試料移送装置におけるポート部を示す平面図である。It is a top view which shows the port part in the biological sample transfer apparatus which concerns on 1st Embodiment. 図５のＢ−Ｂ線断面図である。FIG. 6 is a sectional view taken along line B-B in FIG. 5. 第１実施形態の生体試料移送装置を用いた生体試料移送方法における第１閉止工程及び吸引具接続工程を示す図である。It is a figure which shows the 1st closing process and suction tool connection process in the biological sample transfer method using the biological sample transfer apparatus of 1st Embodiment. 第１実施形態の生体試料移送装置を用いた生体試料移送方法における吸引工程を示す図である。It is a figure which shows the suction process in the biological sample transfer method using the biological sample transfer apparatus of 1st Embodiment. 第１実施形態の生体試料移送装置を用いた生体試料移送方法における第２閉止工程、第１開放工程及び気体供給工程を示す図である。It is a figure which shows the 2nd closing process in the biological sample transfer method using the biological sample transfer apparatus of 1st Embodiment, a 1st open process, and a gas supply process. 第１実施形態の生体試料移送装置を用いた生体試料移送方法における生体試料貯留工程を示す図である。It is a figure which shows the biological sample storage process in the biological sample transfer method using the biological sample transfer apparatus of 1st Embodiment. 第１実施形態の生体試料移送装置を用いた生体試料移送方法の生体試料移送工程において、生体試料貯留容器を生体試料保存容器よりも上方に位置させた状態を示した図である。It is the figure which showed the state which located the biological sample storage container above the biological sample storage container in the biological sample transfer process of the biological sample transfer method using the biological sample transfer apparatus of 1st Embodiment. 第１実施形態の生体試料移送装置を用いた生体試料移送方法の生体試料移送工程において、上流側閉止部及び下流側閉止部を開放した状態を示す図である。It is a figure which shows the state which open | released the upstream closing part and the downstream closing part in the biological sample transfer process of the biological sample transfer method using the biological sample transfer apparatus of 1st Embodiment. 第２実施形態に係る生体試料移送装置の全体構成を示す図である。It is a figure which shows the whole structure of the biological sample transfer apparatus which concerns on 2nd Embodiment. 第２実施形態に係る生体試料移送装置を用いた生体試料移送方法における吸引工程を示す図である。It is a figure which shows the suction process in the biological sample transfer method using the biological sample transfer apparatus which concerns on 2nd Embodiment. 第２実施形態に係る生体試料移送装置を用いた生体試料移送方法における細胞導入工程を示す図である。It is a figure which shows the cell introduction process in the biological sample transfer method using the biological sample transfer apparatus which concerns on 2nd Embodiment. 第３実施形態に係る生体試料移送装置の全体構成を示す図である。It is a figure which shows the whole structure of the biological sample transfer apparatus which concerns on 3rd Embodiment. 第４実施形態に係る生体試料移送装置の全体構成を示す図である。It is a figure which shows the whole structure of the biological sample transfer apparatus which concerns on 4th Embodiment. 第４実施形態に係る生体試料移送装置における一方弁の断面図である。It is sectional drawing of the one-way valve in the biological sample transfer apparatus which concerns on 4th Embodiment. 第５実施形態に係る生体試料移送装置の全体構成を示す図であり、生体試料貯留容器を連結部材から分離した状態を示す図である。It is a figure which shows the whole structure of the biological sample transfer apparatus which concerns on 5th Embodiment, and is a figure which shows the state which isolate | separated the biological sample storage container from the connection member. 第５実施形態に係る生体試料移送装置の全体構成を示す図であり、生体試料貯留容器を連結した状態を示す図である。It is a figure which shows the whole structure of the biological sample transfer apparatus which concerns on 5th Embodiment, and is a figure which shows the state which connected the biological sample storage container.

以下、本発明の生体試料移送装置の好ましい各実施形態について、図面を参照しながら説明する。本発明の生体試料移送装置は、幹細胞や臍帯血等の細胞や血液から調製された血清等の液性成分を主体とした生体試料を凍結保存する場合に、採取された生体試料を無菌的に生体試料保存容器に導入するために用いられる。
まず、第１実施形態の生体試料移送装置１について、図１〜図６を参照しながら説明する。
第１実施形態の生体試料移送装置１は、生体試料貯留容器２と、生体試料保存容器３と、生体試料貯留容器２と生体試料保存容器３とを接続する接続チューブ４と、この接続チューブ４に取り付けられるポート部５と、上流側閉止部６と、下流側閉止部７と、を備える。 Hereinafter, preferred embodiments of the biological sample transfer device of the present invention will be described with reference to the drawings. The biological sample transfer device of the present invention is a method for aseptically collecting a collected biological sample when cryopreserving a biological sample mainly composed of a liquid component such as serum prepared from cells or blood such as stem cells and umbilical cord blood. Used for introduction into a biological sample storage container.
First, the biological sample transfer apparatus 1 of 1st Embodiment is demonstrated, referring FIGS.
The biological sample transfer device 1 according to the first embodiment includes a biological sample storage container 2, a biological sample storage container 3, a connection tube 4 that connects the biological sample storage container 2 and the biological sample storage container 3, and the connection tube 4. A port portion 5 attached to the upstream side, an upstream side closing portion 6, and a downstream side closing portion 7.

生体試料貯留容器２は、ポリ塩化ビニル等の可撓性を有する熱可塑性樹脂からなる２枚のシート材の周縁部の大部分が接合され、これら２枚のシート材の間に生体試料貯留部２１が形成された、いわゆる血液バッグにより構成される。この生体試料貯留容器２は、上述の生体試料貯留部２１と、この生体試料貯留部２１に生体試料を導入する生体試料導入部２２と、生体試料貯留部２１に貯留された生体試料を導出する生体試料導出部２３と、を備える。   In the biological sample storage container 2, most of the peripheral portions of two sheet materials made of a flexible thermoplastic resin such as polyvinyl chloride are joined, and the biological sample storage portion is interposed between these two sheet materials. 21 is formed by a so-called blood bag. The biological sample storage container 2 derives the biological sample storage unit 21, the biological sample introduction unit 22 that introduces the biological sample into the biological sample storage unit 21, and the biological sample stored in the biological sample storage unit 21. A biological sample derivation unit 23.

生体試料貯留部２１には、細胞等の生体試料が貯留される。
生体試料導入部２２は、２枚のシート材の周縁部の一部を非接合とすることで形成された生体試料導入路２２１と、一端側が生体試料導入路２２１に接続された生体試料導入チューブ２２２と、この生体試料導入チューブ２２２の他端部に取り付けられた接続部材２２３と、生体試料導入チューブ２２２に取り付けられこの生体試料導入チューブ２２２における流体の流路を開閉させるクランプ２２４と、を備える。生体試料導入チューブ２２２の他端側には、接続部材２２３を介して、血液等の生体試料が充填された容器や、生体試料から所定の細胞を調製し抽出した容器（バッグ又はシリンジ、図示せず）が接続される。 The biological sample storage unit 21 stores biological samples such as cells.
The biological sample introduction unit 22 includes a biological sample introduction path 221 formed by non-joining part of the peripheral part of two sheets, and a biological sample introduction tube having one end connected to the biological sample introduction path 221. 222, a connection member 223 attached to the other end of the biological sample introduction tube 222, and a clamp 224 attached to the biological sample introduction tube 222 to open and close a fluid flow path in the biological sample introduction tube 222. . The other end of the biological sample introduction tube 222 is connected to a container filled with a biological sample such as blood via a connecting member 223, or a container (bag or syringe, illustrated) prepared and extracted from a biological sample. Connected).

生体試料導出部２３は、２枚のシート材の周縁部の一部を非接合とすることにより形成される。この生体試料導出部２３には、後述の接続チューブ４（上流側チューブ４１）が接続される。   The biological sample lead-out part 23 is formed by non-joining part of the peripheral part of the two sheet materials. A connecting tube 4 (upstream tube 41), which will be described later, is connected to the biological sample outlet 23.

第１実施形態では、図１に示すように、生体試料貯留容器２は、平面視において縦長形状に形成される。そして、生体試料導入部２２及び生体試料導出部２３は、生体試料貯留容器２の長手方向の一端部である上端部側に配置される。また、生体試料貯留部２１の上端縁は、生体試料導出部２３が配置された位置が頂点となるように傾斜した２つのテーパ辺により構成される。   In the first embodiment, as shown in FIG. 1, the biological sample storage container 2 is formed in a vertically long shape in plan view. The biological sample introduction unit 22 and the biological sample derivation unit 23 are arranged on the upper end side that is one end portion of the biological sample storage container 2 in the longitudinal direction. Further, the upper end edge of the biological sample storage unit 21 is configured by two tapered sides that are inclined so that the position where the biological sample deriving unit 23 is disposed is the apex.

図２は、生体試料保存容器３の斜視図であり、図３は平面図である。尚、図２及び図３においては、カバー部３１４を省略して示している。
生体試料保存容器３は、図１〜図３に示すように、保存容器本体３１と、筒状部材３２と、を備える。
保存容器本体３１は、ＥＶＡ樹脂（エチレン−酢酸ビニル共重合樹脂）等の可撓性を有する熱可塑性樹脂からなる２枚のシート状部材それぞれの周縁部の大部分及び所定の部位（後述の第１生体試料収容室と第２生体試料収容室との境界部分）が接合されて構成される。この保存容器本体３１には、生体試料収容室３１１と、生体試料導入口３１２と、生体試料導出口３１３と、カバー部３１４と、が形成される。 FIG. 2 is a perspective view of the biological sample storage container 3, and FIG. 3 is a plan view. 2 and 3, the cover portion 314 is omitted.
As illustrated in FIGS. 1 to 3, the biological sample storage container 3 includes a storage container main body 31 and a cylindrical member 32.
The storage container body 31 includes most of the peripheral portions of two sheet-like members made of a thermoplastic resin having flexibility such as EVA resin (ethylene-vinyl acetate copolymer resin) and a predetermined portion (described later). The boundary portion between the first biological sample storage chamber and the second biological sample storage chamber) is joined. In the storage container main body 31, a biological sample storage chamber 311, a biological sample introduction port 312, a biological sample outlet 313, and a cover part 314 are formed.

生体試料収容室３１１は、シート状部材が立体成形されて形成される。より具体的には、まず、２枚のシート状部材のうちの一方のシート状部材に真空成形等が施されて立体形状が形成される。次いで、この立体形状が形成された一方のシート状部材に、平面状の他方のシート状部材が接合される。これにより、立体形状を有する生体試料収容室３１１が形成される。
第１実施形態では、生体試料収容室３１１は、図２〜図４に示すように、比較的多くの量（例えば、１０ｍｌ〜３０ｍｌ）の生体試料を収容可能な第１生体試料収容室３１１ａと、この第１生体試料収容室３１１ａよりも少ない量（例えば、３ｍｌ〜１０ｍｌ）の生体試料を収容可能な第２生体試料収容室３１１ｂと、これら第１生体試料収容室３１１ａと第２生体試料収容室３１１ｂとを連通させる連通部３１１ｃと、を備える。 The biological sample storage chamber 311 is formed by three-dimensionally molding a sheet-like member. More specifically, first, one of the two sheet-like members is subjected to vacuum forming or the like to form a three-dimensional shape. Next, the other planar sheet-like member is joined to one sheet-like member formed with this three-dimensional shape. Thereby, the biological sample storage chamber 311 having a three-dimensional shape is formed.
In the first embodiment, the biological sample storage chamber 311 includes a first biological sample storage chamber 311a that can store a relatively large amount (for example, 10 ml to 30 ml) of a biological sample, as shown in FIGS. The second biological sample storage chamber 311b that can store a smaller amount (for example, 3 ml to 10 ml) of the biological sample than the first biological sample storage chamber 311a, and the first biological sample storage chamber 311a and the second biological sample storage A communication portion 311c for communicating with the chamber 311b.

第１実施形態では、第１生体試料収容室３１１ａ及び第２生体試料収容室３１１ｂは、図２及び図３に示すように、保存容器本体３１の幅方向ＷＤに隣り合って配置される。また、連通部３１１ｃは、保存容器本体３１の高さ方向ＨＤの一端側である上端側及び他端側である下端側の２箇所に設けられる。   In the first embodiment, the first biological sample storage chamber 311a and the second biological sample storage chamber 311b are arranged adjacent to each other in the width direction WD of the storage container main body 31, as shown in FIGS. Moreover, the communication part 311c is provided in two places, the upper end side which is the one end side of the height direction HD of the storage container main body 31, and the lower end side which is the other end side.

生体試料導入口３１２は、生体試料収容室３１１に生体試料を導入する入口となる。この生体試料導入口３１２は、保存容器本体３１を構成する２枚のシート状部材の周縁部の一部を非接合とすることにより構成される。第１実施形態では、生体試料導入口３１２は、図１及び図２に示すように、保存容器本体３１の幅方向ＷＤの一端側の上端部に設けられる。また、生体試料導入口３１２は、第１生体試料収容室３１１ａに設けられる。   The biological sample inlet 312 serves as an inlet for introducing the biological sample into the biological sample storage chamber 311. The biological sample introduction port 312 is configured by non-joining part of the peripheral portions of the two sheet-like members constituting the storage container main body 31. In the first embodiment, the biological sample inlet 312 is provided at the upper end of one end of the storage container body 31 in the width direction WD as shown in FIGS. 1 and 2. Further, the biological sample introduction port 312 is provided in the first biological sample storage chamber 311a.

生体試料導出口３１３は、生体試料収容室３１１に収容された生体試料を導出する出口となる。この生体試料導出口３１３は、生体試料導入口３１２と同様に、保存容器本体３１を構成する２枚のシート状部材の周縁部の一部を非接合とすることにより構成される。第１実施形態では、生体試料導出口３１３は、第１生体試料収容室３１１ａ及び第２生体試料収容室３１２ｂのそれぞれの上端部に設けられる。
カバー部３１４は、生体試料導出口３１３を覆う。このカバー部３１４は、生体試料導出口３１３から生体試料を取り出す場合に除去される。 The biological sample outlet 313 serves as an outlet for leading out the biological sample stored in the biological sample storage chamber 311. Similar to the biological sample introduction port 312, the biological sample outlet 313 is configured by non-joining part of the peripheral portions of the two sheet-like members that constitute the storage container body 31. In the first embodiment, the biological sample outlet 313 is provided at the upper end of each of the first biological sample storage chamber 311a and the second biological sample storage chamber 312b.
The cover part 314 covers the biological sample outlet 313. The cover part 314 is removed when the biological sample is taken out from the biological sample outlet 313.

筒状部材３２は、生体試料保存容器３の生体試料導出口３１３に配置される。この筒状部材３２は、筒本体３２１と、隔膜部３２２と、一対のガード部３２３と、を備える。   The cylindrical member 32 is disposed in the biological sample outlet 313 of the biological sample storage container 3. The tubular member 32 includes a tubular body 321, a diaphragm part 322, and a pair of guard parts 323.

筒本体３２１は、円筒形状に構成される。
隔膜部３２２は、筒本体３２１の内部に配置され、筒本体３２１を閉鎖する。この隔膜部３２２は、肉薄の円板状に形成され、外力の作用によって穿孔される程度の強度を備える。ガード部３２３は、筒本体３２１の生体試料収容室３１１側の端部から、筒本体３２１の軸方向に延びるように形成される。このガード部３２３は筒本体３２１と連続的に形成される。
以上の筒状部材３２は、ＥＶＡ樹脂等の熱可塑性樹脂により形成される。 The cylinder main body 321 is configured in a cylindrical shape.
The diaphragm part 322 is disposed inside the cylinder body 321 and closes the cylinder body 321. The diaphragm part 322 is formed in a thin disk shape and has a strength enough to be perforated by the action of an external force. The guard part 323 is formed so as to extend in the axial direction of the cylinder main body 321 from the end of the cylinder main body 321 on the biological sample storage chamber 311 side. The guard portion 323 is formed continuously with the cylinder main body 321.
The above cylindrical member 32 is formed of a thermoplastic resin such as EVA resin.

以上の生体試料保存容器３は、生体試料導入口３１２にのみ液体及び気体の流通が可能な通液部が形成されており、生体試料導出口３１３は、筒状部材３２（隔膜部３２２）により閉止されている。また、生体試料収容室３１１は、立体成形により形成されており、外力が加えられない状態では、立体形状を維持している。   In the biological sample storage container 3 described above, a liquid passing portion capable of flowing liquid and gas is formed only at the biological sample introduction port 312, and the biological sample outlet 313 is formed by the cylindrical member 32 (diaphragm portion 322). It is closed. In addition, the biological sample storage chamber 311 is formed by three-dimensional molding, and maintains a three-dimensional shape when no external force is applied.

接続チューブ４は、ＥＶＡ樹脂等の熱可塑性樹脂により構成され、生体試料貯留容器２と生体試料保存容器３とを無菌的かつ気密に接続する。この接続チューブ４は、上流側チューブ４１、及び下流側チューブ４２により構成される。上流側チューブ４１は、一端部が生体試料貯留容器２の生体試料導出部２３に接続され、他端部が後述のポート部５に接続される。
下流側チューブ４２は、一端部がポート部５に接続され、他端部が生体試料保存容器３の生体試料導入口３１２に接続される。 The connection tube 4 is made of a thermoplastic resin such as EVA resin, and connects the biological sample storage container 2 and the biological sample storage container 3 in an aseptic and airtight manner. The connection tube 4 includes an upstream tube 41 and a downstream tube 42. One end of the upstream tube 41 is connected to the biological sample outlet 23 of the biological sample storage container 2, and the other end is connected to a port unit 5 described later.
The downstream tube 42 has one end connected to the port unit 5 and the other end connected to the biological sample inlet 312 of the biological sample storage container 3.

ポート部５は、生体試料貯留容器２、生体試料保存容器３及び接続チューブ４の気密状態を維持しつつ、接続チューブ４の内部の気体を外部に導出可能に構成される。
第１実施形態では、ポート部５は、図１及び図５に示すように、上流側チューブ４１と下流側チューブ４２との間に設けられ、これら上流側チューブ４１と下流側チューブ４２とを接続する。このポート部５は、セプタム５１と、このセプタム５１に形成された第１接続口５２及び第２接続口５３と、を備える。 The port unit 5 is configured to be able to lead the gas inside the connection tube 4 to the outside while maintaining the airtight state of the biological sample storage container 2, the biological sample storage container 3, and the connection tube 4.
In the first embodiment, as shown in FIGS. 1 and 5, the port portion 5 is provided between the upstream tube 41 and the downstream tube 42, and connects the upstream tube 41 and the downstream tube 42. To do. The port portion 5 includes a septum 51 and a first connection port 52 and a second connection port 53 formed in the septum 51.

セプタム５１は、図５及び図６に示すように、円形の開口が形成された本体部５１１と、この本体部５１１の開口に配置される膜状又は板状に形成された弾性部材５１２と、この弾性部材５１２に形成されたスリット部５１３と、を備える。
第１接続口５２には、上流側チューブ４１の他端部が接続される。第２接続口５３には、下流側チューブ４２の一端部が接続される。 As shown in FIGS. 5 and 6, the septum 51 includes a main body portion 511 having a circular opening, an elastic member 512 formed in a film shape or a plate shape disposed in the opening of the main body portion 511, and A slit portion 513 formed in the elastic member 512.
The other end of the upstream tube 41 is connected to the first connection port 52. One end of the downstream tube 42 is connected to the second connection port 53.

以上のポート部５によれば、セプタム５１のスリット部５１３にシリンジ等の吸引具の先端部を挿入すると、弾性部材５１２が弾性変形することでスリット部５１３が開放される。これにより、吸引具により接続チューブ４の内部の気体を吸引して外部に導出可能となる。また、吸引具の先端部をスリット部５１３から抜き取ると、弾性部材５１２の弾性回復力によりスリット部５１３は閉鎖される。   According to the port portion 5 described above, when the distal end portion of a suction tool such as a syringe is inserted into the slit portion 513 of the septum 51, the elastic member 512 is elastically deformed to open the slit portion 513. Thereby, the gas inside the connection tube 4 can be sucked by the suction tool and led out to the outside. Further, when the distal end portion of the suction tool is extracted from the slit portion 513, the slit portion 513 is closed by the elastic recovery force of the elastic member 512.

上流側閉止部６は、ポート部５よりも生体試料貯留容器２側に設けられる。第１実施形態では、上流側閉止部６は、図１に示すように、合成樹脂製のクランプにより構成され、上流側チューブ４１に取り付けられる。
以上の上流側閉止部６によれば、クランプにより上流側チューブ４１を挟み込むことで、上流側チューブ４１における流体（液体及び気体）の流通を遮断できる。また、クランプによる上流側チューブ４１の挟み込みを解除することで、上流側チューブ４１における流体の流通を許容できる。
尚、「ポート部５よりも生体試料貯留容器２側に設けられる」とは、本実施形態のようにポート部５と生体試料貯留容器２との間に設けられる場合に限られず、生体試料貯留容器２における生体試料導入部２２に設けられる場合も含む。 The upstream closing portion 6 is provided closer to the biological sample storage container 2 than the port portion 5. In the first embodiment, as shown in FIG. 1, the upstream closing portion 6 is configured by a synthetic resin clamp and is attached to the upstream tube 41.
According to the upstream closing portion 6 described above, the flow of the fluid (liquid and gas) in the upstream tube 41 can be blocked by sandwiching the upstream tube 41 with the clamp. Further, the fluid flow in the upstream tube 41 can be allowed by releasing the clamping of the upstream tube 41 by the clamp.
The phrase “provided on the biological sample storage container 2 side with respect to the port unit 5” is not limited to the case of being provided between the port unit 5 and the biological sample storage container 2 as in the present embodiment. The case where it is provided in the biological sample introduction part 22 in the container 2 is included.

下流側閉止部７は、ポート部５よりも生体試料保存容器３側に設けられる。第１実施形態では、下流側閉止部７は、図１に示すように、合成樹脂製のクランプにより構成され、下流側チューブ４２に取り付けられる。
以上の上流側閉止部６によれば、クランプにより下流側チューブ４２を挟み込むことで、下流側チューブ４２における流体（液体及び気体）の流通を遮断できる。また、クランプによる下流側チューブ４２の挟み込みを解除することで、下流側チューブ４２における流体の流通を許容できる。 The downstream closing part 7 is provided closer to the biological sample storage container 3 than the port part 5. In the first embodiment, the downstream closing portion 7 is configured by a synthetic resin clamp and is attached to the downstream tube 42 as shown in FIG. 1.
According to the upstream closing portion 6 described above, the flow of the fluid (liquid and gas) in the downstream tube 42 can be blocked by sandwiching the downstream tube 42 with the clamp. Further, by releasing the clamping of the downstream side tube 42 by the clamp, the fluid flow in the downstream side tube 42 can be allowed.

次に、第１実施形態の生体試料移送装置１を用いた生体試料移送方法の一実施態様について、図７〜図１２を参照しながら説明する。
本実施態様の生体試料移送方法は、第１閉止工程Ｓ１と、吸引具接続工程Ｓ２と、吸引工程Ｓ３と、第２閉止工程Ｓ４と、第１開放工程Ｓ５と、気体供給工程Ｓ６と、吸引具取り外し工程Ｓ７と、生体試料貯留工程Ｓ８と、生体試料移送工程Ｓ９と、を備える。 Next, an embodiment of a biological sample transfer method using the biological sample transfer apparatus 1 of the first embodiment will be described with reference to FIGS.
The biological sample transfer method of this embodiment includes a first closing step S1, a suction tool connecting step S2, a suction step S3, a second closing step S4, a first opening step S5, a gas supply step S6, and a suction. A tool removal step S7, a biological sample storage step S8, and a biological sample transfer step S9 are provided.

第１閉止工程Ｓ１では、図７に示すように、上流側閉止部６が閉止される。これにより、上流側チューブ４１における流体の流通が遮断される。この第１閉止工程Ｓ１においては、下流側閉止部７は、開放されており、下流側チューブ４２における流体の流通は許容される。また、第１閉止工程Ｓ１では、生体試料導入部２２のクランプ２２４も閉止される。   In the first closing step S1, the upstream side closing portion 6 is closed as shown in FIG. Thereby, the flow of fluid in the upstream tube 41 is blocked. In the first closing step S <b> 1, the downstream side closing portion 7 is opened, and fluid flow in the downstream side tube 42 is allowed. In the first closing step S1, the clamp 224 of the biological sample introduction unit 22 is also closed.

吸引具接続工程Ｓ２では、図７に示すように、ポート部５に、吸引具としてのシリンジ１００が接続される。より具体的には、吸引具接続工程Ｓ２では、シリンダ部１１０にピストン１２０を押し込んだ状態のシリンジ１００の先端部を、セプタム５１のスリット部５１３に押し込んで、ポート部５にシリンジ１００を接続する。   In the suction tool connection step S <b> 2, as shown in FIG. 7, a syringe 100 as a suction tool is connected to the port portion 5. More specifically, in the suction tool connection step S <b> 2, the distal end portion of the syringe 100 with the piston 120 pushed into the cylinder portion 110 is pushed into the slit portion 513 of the septum 51, and the syringe 100 is connected to the port portion 5. .

吸引工程Ｓ３では、図８に示すように、シリンジ１００により生体試料保存容器３の内部の空気が吸引される。より具体的には、吸引工程Ｓ３では、シリンダ部１１０に押し込まれた状態のピストン１２０が引き出される。すると、上流側閉止部６により上流側チューブ４１は閉止されているので、シリンダ部１１０の内部には、下流側チューブ４２の内部に存在する空気、及び生体試料保存容器３の生体試料収容室３１１に存在する空気が吸引される。その結果、図８に示すように、立体形状を有する生体試料収容室３１１が潰れるように変形し、また、生体試料保存容器３の内部及び接続チューブ４の内部に陰圧が生じる。   In the suction step S3, the air inside the biological sample storage container 3 is sucked by the syringe 100 as shown in FIG. More specifically, in the suction step S3, the piston 120 that has been pushed into the cylinder part 110 is pulled out. Then, since the upstream side tube 41 is closed by the upstream side closing part 6, the air existing inside the downstream side tube 42 and the biological sample storage chamber 311 of the biological sample storage container 3 are inside the cylinder part 110. The air present in the air is aspirated. As a result, as shown in FIG. 8, the biological sample storage chamber 311 having a three-dimensional shape is deformed so as to be crushed, and negative pressure is generated inside the biological sample storage container 3 and inside the connection tube 4.

第２閉止工程Ｓ４では、図９に示すように、下流側閉止部７が閉止される。また、第１開放工程Ｓ５では、上流側閉止部６が開放される。これにより、下流側チューブ４２における流体の流通が遮断され、上流側チューブ４１における流体の流通が許容される。   In the second closing step S4, as shown in FIG. 9, the downstream side closing portion 7 is closed. In the first opening step S5, the upstream closing portion 6 is opened. Thereby, the flow of fluid in the downstream tube 42 is blocked, and the flow of fluid in the upstream tube 41 is allowed.

気体供給工程Ｓ６では、図９に示すように、シリンジ１００に吸引された空気が生体試料貯留容器２側に供給される。より具体的には、気体供給工程Ｓ６では、吸引工程Ｓ３において引き出されていたピストン１２０を押し込むことにより、シリンダ部１１０の内部に吸引されていた空気が生体試料貯留容器２側に供給される。   In the gas supply step S6, as shown in FIG. 9, the air sucked into the syringe 100 is supplied to the biological sample storage container 2 side. More specifically, in the gas supply step S6, the air that has been sucked into the cylinder portion 110 is supplied to the biological sample storage container 2 side by pushing in the piston 120 that has been pulled out in the suction step S3.

吸引具取り外し工程Ｓ７では、シリンジ１００がポート部５から取り外される。これにより、ポート部５は再び密閉される。   In the suction tool removal step S <b> 7, the syringe 100 is removed from the port unit 5. Thereby, the port part 5 is sealed again.

生体試料貯留工程Ｓ８では、図１０に示すように、クランプ２２４が開放され、生体試料貯留容器２に生体試料が貯留される。ここで、生体試料貯留容器２には、気体供給工程Ｓ６において、空気が供給されている。これにより、生体試料貯留容器２が血液バッグのような立体成形されていない容器により構成されていた場合には、無菌状態が保たれた空気を生体試料貯留容器２に供給することで、生体試料貯留容器２を若干ふくらませられる。よって、生体試料貯留容器２に貯留された生体試料に、例えば凍害保護剤のような添加剤が添加された場合に、生体試料と添加剤とを好適に混合できる。この場合、生体試料貯留容器２には、空気が導入された後に生体試料が導入されるので、生体試料貯留容器２に貯留された生体試料は泡立たない。また、生体試料貯留容器２に生体試料が貯留された後に空気が導入された場合であっても、空気は生体試料貯留容器２の上部に設けられた生体試料導出部２３から生体試料貯留部２１に導入されるので、生体試料貯留部２１への空気の導入により生体試料は泡立たない。
尚、この生体試料貯留工程Ｓ８においては、図１０に示すように、第１開放工程Ｓ５において開放した上流側閉止部６を再び閉止してもよい。 In the biological sample storage step S <b> 8, as shown in FIG. 10, the clamp 224 is opened, and the biological sample is stored in the biological sample storage container 2. Here, air is supplied to the biological sample storage container 2 in the gas supply step S6. Accordingly, when the biological sample storage container 2 is configured by a container that is not three-dimensionally shaped, such as a blood bag, the biological sample storage container 2 is supplied with air that is maintained in a sterile state. The storage container 2 can be slightly inflated. Therefore, when an additive such as a frost damage protective agent is added to the biological sample stored in the biological sample storage container 2, the biological sample and the additive can be suitably mixed. In this case, since the biological sample is introduced into the biological sample storage container 2 after air is introduced, the biological sample stored in the biological sample storage container 2 does not foam. Further, even when air is introduced after the biological sample is stored in the biological sample storage container 2, the air is supplied from the biological sample lead-out unit 23 provided at the upper part of the biological sample storage container 2 to the biological sample storage unit 21. Therefore, the biological sample does not foam due to the introduction of air into the biological sample reservoir 21.
In this biological sample storing step S8, as shown in FIG. 10, the upstream closing portion 6 opened in the first opening step S5 may be closed again.

生体試料移送工程Ｓ９では、図１１及び図１２に示すように、上流側閉止部６が開放されて生体試料貯留容器２に収容された細胞が生体試料保存容器３に導入される。より具体的には、生体試料移送工程Ｓ９では、まず、図１１に示すように、生体試料貯留容器２を、生体試料導出部２３が下方を向くようにした状態で、生体試料保存容器３よりも上方に位置させる。次いで、図１２に示すように、下流側閉止部７を開放する。また、生体試料貯留工程Ｓ８において上流側閉止部６を閉止した場合には、この生体試料移送工程Ｓ９において上流側閉止部６も開放する。すると、生体試料貯留容器２と生体試料保存容器３との間の落差圧により、生体試料貯留容器２に貯留された生体試料は、接続チューブ４を介して生体試料保存容器３に移送される。   In the biological sample transfer step S <b> 9, as shown in FIGS. 11 and 12, the cells stored in the biological sample storage container 2 with the upstream closing portion 6 opened are introduced into the biological sample storage container 3. More specifically, in the biological sample transfer step S9, first, as shown in FIG. 11, the biological sample storage container 2 is moved from the biological sample storage container 3 with the biological sample outlet 23 facing downward. Is also positioned above. Next, as shown in FIG. 12, the downstream closing portion 7 is opened. When the upstream closing unit 6 is closed in the biological sample storage step S8, the upstream closing unit 6 is also opened in the biological sample transfer step S9. Then, the biological sample stored in the biological sample storage container 2 is transferred to the biological sample storage container 3 through the connection tube 4 due to the drop pressure between the biological sample storage container 2 and the biological sample storage container 3.

ここで、本実施態様では、吸引工程Ｓ３において、生体試料収容室３１１の内部の空気が吸引されている。これにより、生体試料収容室３１１に存在する空気と生体試料とを置換する作業を行うことなく、生体試料貯留容器２から生体試料保存容器３に生体試料を移送できる。よって、生体試料貯留容器２から生体試料保存容器３に生体試料を移送する場合に、生体試料に泡立ちが生じることを防げるので、生体試料に悪影響を与えにくい。また、生体試料に泡立ちが生じないので、生体試料貯留容器に生体試料を残存させることなく生体試料保存容器に移送できる。
また、生体試料移送装置１の内部空間に陰圧が生じているため、生体試料貯留容器２から生体試料保存容器３への生体試料の移送をより効率良く行える。 Here, in this embodiment, the air inside the biological sample storage chamber 311 is sucked in the suction step S3. Accordingly, the biological sample can be transferred from the biological sample storage container 2 to the biological sample storage container 3 without performing an operation of replacing the air present in the biological sample storage chamber 311 with the biological sample. Therefore, when the biological sample is transferred from the biological sample storage container 2 to the biological sample storage container 3, it is possible to prevent foaming from occurring in the biological sample, and thus it is difficult to adversely affect the biological sample. In addition, since bubbling does not occur in the biological sample, it can be transferred to the biological sample storage container without leaving the biological sample in the biological sample storage container.
Further, since a negative pressure is generated in the internal space of the biological sample transfer device 1, the biological sample can be more efficiently transferred from the biological sample storage container 2 to the biological sample storage container 3.

尚、上述した実施態様では、生体試料移送方法を、気体供給工程Ｓ６を含んで構成したが、この気体供給工程Ｓ６を設けずに生体試料移送方法を行なってもよい。この場合、吸引工程Ｓ３の後に、吸引具取り外し工程Ｓ７、生体試料貯留工程Ｓ８及び生体試料移送工程Ｓ９を実施すればよい。   In the above-described embodiment, the biological sample transfer method includes the gas supply step S6. However, the biological sample transfer method may be performed without providing the gas supply step S6. In this case, the suction tool removal step S7, the biological sample storage step S8, and the biological sample transfer step S9 may be performed after the suction step S3.

以上説明した第１実施形態の生体試料移送装置１及びこの生体試料移送装置１を用いた生体試料移送方法によれば、以下のような効果を奏する。   According to the biological sample transfer device 1 of the first embodiment and the biological sample transfer method using the biological sample transfer device 1 described above, the following effects are obtained.

（１）生体試料移送装置１を、生体試料貯留容器２と生体試料保存容器３とを接続する接続チューブ４に設けられ、気密状態を維持しつつ接続チューブ４の内部の気体を外部に導出可能なポート部５と、このポート部５よりも生体試料貯留容器２側に設けられた上流側閉止部６と、を含んで構成した。これにより、上流側閉止部６を閉止した状態でポート部５から生体試料保存容器３の生体試料収容室３１１に存在する空気を吸引できるので、生体試料収容室３１１に存在する空気と生体試料とを置換する作業を行うことなく、生体試料貯留容器２から生体試料保存容器３に生体試料を導入できる。よって、生体試料貯留容器２から生体試料保存容器３に生体試料を導入する場合に、生体試料に泡立ちが生じることを防げるので、生体試料に悪影響を与えにくい。   (1) The biological sample transfer device 1 is provided in the connection tube 4 that connects the biological sample storage container 2 and the biological sample storage container 3, and the gas inside the connection tube 4 can be led out while maintaining an airtight state. The port portion 5 and the upstream closing portion 6 provided on the biological sample storage container 2 side with respect to the port portion 5 are configured. As a result, the air present in the biological sample storage chamber 311 of the biological sample storage container 3 can be aspirated from the port portion 5 with the upstream side closing portion 6 closed, so that the air present in the biological sample storage chamber 311 and the biological sample The biological sample can be introduced from the biological sample storage container 2 to the biological sample storage container 3 without performing the operation of replacing the. Therefore, when the biological sample is introduced from the biological sample storage container 2 to the biological sample storage container 3, it is possible to prevent the biological sample from being bubbled, so that the biological sample is hardly adversely affected.

（２）生体試料移送装置１を、ポート部５よりも生体試料保存容器３側に設けた下流側閉止部７を含んで構成した。これにより、吸引工程Ｓ３において生体試料収容室３１１の内部の空気を吸引した後、下流側閉止部７を閉止すると共に上流側閉止部６を開放し、その後、気体供給工程Ｓ６において、吸引した空気を生体試料貯留容器２側に供給できる。これにより、生体試料貯留容器２が血液バッグのような立体成形されていない容器により構成されていた場合には、無菌状態が保たれた空気を生体試料貯留容器２に供給することで、生体試料貯留容器２を若干ふくらませられる。よって、生体試料貯留容器２に貯留された生体試料に、例えば凍害保護剤のような添加剤が添加された場合に、生体試料と添加剤とを好適に混合できる。   (2) The biological sample transfer apparatus 1 is configured to include the downstream closing portion 7 provided on the biological sample storage container 3 side with respect to the port portion 5. Thus, after the air inside the biological sample storage chamber 311 is aspirated in the aspirating step S3, the downstream closing portion 7 is closed and the upstream closing portion 6 is opened, and then the aspirated air in the gas supply step S6. Can be supplied to the biological sample storage container 2 side. Accordingly, when the biological sample storage container 2 is configured by a container that is not three-dimensionally shaped, such as a blood bag, the biological sample storage container 2 is supplied with air that is maintained in a sterile state. The storage container 2 can be slightly inflated. Therefore, when an additive such as a frost damage protective agent is added to the biological sample stored in the biological sample storage container 2, the biological sample and the additive can be suitably mixed.

（３）ポート部５を、弾性部材５１２と、この弾性部材５１２に形成されたスリット部５１３と、を有するセプタム５１を含んで構成した。これにより、セプタム５１にシリンジ１００の先端部を挿入することでスリット部５１３を開放させられるので、シリンジ１００により接続チューブ４の内部の気体を吸引して外部に導出できる。また、シリンジ１００の先端部をスリット部５１３から抜き取ることで、スリット部５１３を閉鎖させて密閉できる。よって、生体試料貯留容器２、生体試料保存容器３及び接続チューブ４の気密状態を維持しつつ、接続チューブ４の内部の気体を外部に導出可能なポート部５を、簡易な構成で実現できる。   (3) The port portion 5 is configured to include a septum 51 having an elastic member 512 and a slit portion 513 formed in the elastic member 512. Thereby, since the slit part 513 is open | released by inserting the front-end | tip part of the syringe 100 in the septum 51, the gas inside the connection tube 4 can be attracted | sucked with the syringe 100 and it can guide | induced to the exterior. Moreover, the slit part 513 can be closed and sealed by extracting the tip part of the syringe 100 from the slit part 513. Therefore, it is possible to realize the port portion 5 that can lead out the gas inside the connection tube 4 to the outside while maintaining the airtight state of the biological sample storage container 2, the biological sample storage container 3, and the connection tube 4.

次に、本発明の第２実施形態に係る生体試料移送装置１Ａについて、図１３〜図１５を参照しながら説明する。尚、第２実施形態以降の説明にあたって、同一構成要件については同一符号を付し、その説明を省略もしくは簡略化する。   Next, a biological sample transfer apparatus 1A according to a second embodiment of the present invention will be described with reference to FIGS. In the description after the second embodiment, the same constituent elements are denoted by the same reference numerals, and the description thereof is omitted or simplified.

第２実施形態の生体試料移送装置１Ａは、上流側閉止部及び下流側閉止部が、三方活栓８により構成されている点で、第１実施形態の生体試料移送装置１と異なる。
三方活栓８は、第１接続口８１と、第２接続口８２と、第３接続口８３と、これらの３つの接続口のうちの２つの接続口を連通させ１つの接続口を閉鎖する切替部材８４と、を備える。 The biological sample transfer apparatus 1A according to the second embodiment is different from the biological sample transfer apparatus 1 according to the first embodiment in that the upstream side closing part and the downstream side closing part are constituted by three-way stopcocks 8.
The three-way stopcock 8 switches the first connection port 81, the second connection port 82, the third connection port 83, and two of these three connection ports to communicate with each other and closes one connection port. Member 84.

第２実施形態では、第１接続口８１は、上流側チューブ４１に接続され、第２接続口８２は、下流側チューブ４２に接続される。そして、第３接続口８３は、ポート部としてのセプタム５１Ａに接続される。
切替部材８４は、第２接続口８２（生体試料保存容器３）と第３接続口８３（セプタム５１Ａ）とを連通させ第１接続口８１を閉鎖する第１位置（図１４参照）、第１接続口８１（生体試料貯留容器２）と第２接続口８２（生体試料保存容器３）とを連通させ第３接続口８３を閉鎖する第２位置（図１５参照）、及び第３接続口８３（セプタム５１Ａ）と第１接続口８１（生体試料貯留容器２）とを連通させ第２接続口８２を閉鎖する第３位置（図示せず）を切替可能に構成される。 In the second embodiment, the first connection port 81 is connected to the upstream tube 41, and the second connection port 82 is connected to the downstream tube 42. And the 3rd connection port 83 is connected to the septum 51A as a port part.
The switching member 84 communicates the second connection port 82 (biological sample storage container 3) and the third connection port 83 (septum 51A) and closes the first connection port 81 (see FIG. 14), first. A second position (see FIG. 15) where the connection port 81 (biological sample storage container 2) and the second connection port 82 (biological sample storage container 3) communicate with each other and the third connection port 83 is closed, and the third connection port 83. A third position (not shown) in which the (septum 51A) and the first connection port 81 (biological sample storage container 2) communicate with each other and the second connection port 82 is closed can be switched.

第２実施形態の生体試料移送装置１Ａを用いた生体試料貯留容器２から生体試料保存容器３への生体試料の移送は、以下の手順で行われる。
まず、図１４に示すように、切替部材８４を第２位置に位置させて第１閉止工程Ｓ１を実施し、次いで、シリンジ１００をセプタム５１Ａに挿入して、生体試料保存容器３の生体試料収容室３１１の内部に存在する空気を吸引する（吸引具接続工程Ｓ２及び吸引工程Ｓ３）。次いで、シリンジ１００をセプタム５１Ａから取り外す（吸引具取り外し工程Ｓ７）。次いで、生体試料貯留容器２に生体試料を貯留する（生体試料貯留工程Ｓ８）。次いで、生体試料貯留容器２を生体試料保存容器３よりも上方に位置させた状態で、切替部材８４を第３位置に位置させ、生体試料貯留容器２に貯留された生体試料を生体試料保存容器３に導入する（生体試料移送工程Ｓ９）。 Transfer of the biological sample from the biological sample storage container 2 to the biological sample storage container 3 using the biological sample transfer apparatus 1A of the second embodiment is performed according to the following procedure.
First, as shown in FIG. 14, the switching member 84 is positioned at the second position to perform the first closing step S1, and then the syringe 100 is inserted into the septum 51A to store the biological sample in the biological sample storage container 3. Air present inside the chamber 311 is sucked (suction tool connection step S2 and suction step S3). Next, the syringe 100 is removed from the septum 51A (suction tool removing step S7). Next, the biological sample is stored in the biological sample storage container 2 (biological sample storage step S8). Next, in a state in which the biological sample storage container 2 is positioned above the biological sample storage container 3, the switching member 84 is positioned at the third position, and the biological sample stored in the biological sample storage container 2 is stored in the biological sample storage container. 3 (biological sample transfer step S9).

尚、第２実施形態の三方活栓８を用いた場合、切替部材８４を第１位置から第３位置に切り替える場合には、一旦、第２位置を経由する必要が生じる。そのため、第２実施形態の生体試料移送装置１Ａを用いた生体試料移送方法において、気体供給工程Ｓ６を実施する場合には、上流側チューブ４１に、クランプ等の上流側閉止部を別途設けることが好ましい。これにより、吸引工程Ｓ３の後、クランプ等により上流側チューブ４１を閉止して第２閉止工程Ｓ４を実施し、この状態で切替部材８４を第１位置から第２位置を経由して第３位置に切り替えた後にクランプを開放することで第１開放工程Ｓ５及び気体供給工程Ｓ６を実施できる。   In addition, when the three-way cock 8 of the second embodiment is used, when the switching member 84 is switched from the first position to the third position, it is necessary to temporarily go through the second position. Therefore, in the biological sample transfer method using the biological sample transfer device 1A of the second embodiment, when the gas supply step S6 is performed, the upstream tube 41 may be separately provided with an upstream closing portion such as a clamp. preferable. Thus, after the suction step S3, the upstream tube 41 is closed by a clamp or the like, and the second closing step S4 is performed. In this state, the switching member 84 is moved from the first position to the third position via the second position. The first opening step S5 and the gas supply step S6 can be performed by opening the clamp after switching to.

第２実施形態の生体試料移送装置１Ａによれば、上述した（１）〜（３）の効果を奏する他、以下のような効果を奏する。   According to the biological sample transfer apparatus 1A of the second embodiment, in addition to the effects (1) to (3) described above, the following effects can be obtained.

（４）上流側閉止部及び下流側閉止部を切替部材８４を備える三方活栓８により構成した。これにより、切替部材８４を切り替えることで、流路の切替を容易に行えるので、本発明の生体試料移送方法をより容易に行える。   (4) The upstream closing portion and the downstream closing portion are configured by the three-way cock 8 including the switching member 84. Thereby, since the switching of the flow path can be easily performed by switching the switching member 84, the biological sample transfer method of the present invention can be performed more easily.

次に、本発明の第３実施形態に係る生体試料移送装置１Ｂについて、図１６を参照しながら説明する。
第３実施形態の生体試料移送装置１Ｂは、接続チューブ４Ｂの構成において第１実施形態と異なる。第３実施形態では、接続チューブ４Ｂは、上流側チューブ４１Ｂと、下流側チューブ４２Ｂと、分岐チューブ４３Ｂと、により構成される。 Next, a biological sample transfer device 1B according to a third embodiment of the present invention will be described with reference to FIG.
A biological sample transfer apparatus 1B of the third embodiment is different from the first embodiment in the configuration of the connection tube 4B. In 3rd Embodiment, the connection tube 4B is comprised by the upstream tube 41B, the downstream tube 42B, and the branch tube 43B.

上流側チューブ４１Ｂは、生体試料貯留容器２側に配置され、基端部が生体試料貯留容器２の生体試料導出部２３に接続される。
下流側チューブ４２Ｂは、生体試料保存容器３側に配置され、先端部が生体試料保存容器３の生体試料導入口３１２に接続される。
分岐チューブ４３Ｂは、基端部が上流側チューブ４１Ｂの先端部と下流側チューブ４２Ｂの基端部との接続部分に接続される。そして、この分岐チューブ４３Ｂの先端部には、ポート部５Ｂが取り付けられる。
第３実施形態では、上流側チューブ４１Ｂ、下流側チューブ４２Ｂ及び分岐チューブ４３Ｂは、３つの接続口を有する連結部材４４Ｂにより接続される。 The upstream side tube 41 </ b> B is disposed on the biological sample storage container 2 side, and the proximal end portion is connected to the biological sample outlet 23 of the biological sample storage container 2.
The downstream tube 42 </ b> B is disposed on the biological sample storage container 3 side, and the tip is connected to the biological sample inlet 312 of the biological sample storage container 3.
The branch tube 43B has a proximal end connected to a connection portion between the distal end of the upstream tube 41B and the proximal end of the downstream tube 42B. And the port part 5B is attached to the front-end | tip part of this branch tube 43B.
In the third embodiment, the upstream tube 41B, the downstream tube 42B, and the branch tube 43B are connected by a connecting member 44B having three connection ports.

第３実施形態の生体試料移送装置１Ｂによれば、上述した（１）〜（３）の効果を奏する他、以下のような効果を奏する。   According to the biological sample transfer apparatus 1B of the third embodiment, in addition to the effects (1) to (3) described above, the following effects can be obtained.

（５）接続チューブ４Ｂを、上流側チューブ４１Ｂと、下流側チューブ４２Ｂと、分岐チューブ４３Ｂと、を含んで構成した。これにより、分岐チューブ４３Ｂの先端部にポート部５Ｂを取り付けられるので、生体試料移送装置１Ｂの操作性を向上させられる。   (5) The connection tube 4B includes an upstream tube 41B, a downstream tube 42B, and a branch tube 43B. Thereby, since the port part 5B is attached to the front-end | tip part of the branch tube 43B, the operativity of the biological sample transfer apparatus 1B can be improved.

次に、本発明の第４実施形態に係る生体試料移送装置１Ｃについて、図１７及び図１８を参照しながら説明する。
第４実施形態の生体試料移送装置１Ｃは、ポート部の構成において第３実施形態と異なる。第４実施形態では、ポート部は、接続チューブ４の内部から外部への気体の流通を許容し、外部から内部への気体の流通を遮断する一方弁５Ｃにより構成される。 Next, a biological sample transfer apparatus 1C according to a fourth embodiment of the present invention will be described with reference to FIGS.
A biological sample transfer apparatus 1C of the fourth embodiment differs from that of the third embodiment in the configuration of the port portion. In the fourth embodiment, the port portion is configured by a one-way valve 5 </ b> C that allows gas to flow from the inside to the outside of the connection tube 4 and blocks gas from the outside to the inside.

一方弁５Ｃは、図１８に示すように、分岐チューブ４３Ｃの先端部に接続される第１ケース５４Ｃと、第１ケース５４Ｃに連結される第２ケース５５Ｃと、第１ケース５４Ｃ及び第２ケース５５Ｃの内部に配置される弁体５６Ｃと、を備える。   On the other hand, as shown in FIG. 18, the valve 5C includes a first case 54C connected to the distal end portion of the branch tube 43C, a second case 55C connected to the first case 54C, and the first case 54C and the second case. And a valve body 56C disposed inside 55C.

第１ケース５４Ｃは、一端側が分岐チューブ４３Ｃの先端部に挿入される管状の挿入部５４１と、この挿入部５４１の他端側に設けられた第１嵌合部５４２と、を備える。第１嵌合部５４２は、挿入部５４１よりも太径の筒状に形成される。
第２ケース５５Ｃは、一端側が第１嵌合部５４２の内側に嵌合される筒状の第２嵌合部５５１と、この第２嵌合部５５１の他端側に設けられ、シリンジ等の吸引具が接続される吸引具接続部５５２と、を備える。 54 C of 1st cases are provided with the tubular insertion part 541 by which one end side is inserted in the front-end | tip part of the branch tube 43C, and the 1st fitting part 542 provided in the other end side of this insertion part 541. The first fitting portion 542 is formed in a cylindrical shape having a larger diameter than the insertion portion 541.
The second case 55C is provided on the other end side of the cylindrical second fitting portion 551 with one end side fitted inside the first fitting portion 542 and the second fitting portion 551, such as a syringe. A suction tool connecting portion 552 to which the suction tool is connected.

弁体５６Ｃは、ゴム等の弾性を有する部材により構成され、第２ケース５５Ｃの第２嵌合部５５１の内面側に配置される。この弁体５６Ｃは、一端側が第２嵌合部５５１の内周面に接着され、他端側が吸引具接続部５５２側に向けて突出している。弁体５６Ｃの突出端には、スリット（図示せず）が形成されている。   The valve body 56C is made of an elastic member such as rubber, and is disposed on the inner surface side of the second fitting portion 551 of the second case 55C. One end side of the valve body 56C is bonded to the inner peripheral surface of the second fitting portion 551, and the other end side protrudes toward the suction tool connecting portion 552. A slit (not shown) is formed at the protruding end of the valve body 56C.

以上の一方弁５Ｃによれば、吸引具接続部５５２側から空気を吸引した場合には、吸引具接続部５５２の内部において吸引具側に働く吸引力により弁体５６Ｃのスリットが開口する。これにより、接続チューブ４Ｃから外部に向けて空気が流通する。
一方、吸引具接続部５５２側からの空気の吸引を停止した場合には、弁体５６Ｃのスリットは閉じるため、吸引具接続部５５２側から接続チューブ４Ｃ側に空気は流通しない。 According to the one-way valve 5C described above, when air is sucked from the suction tool connecting portion 552, the slit of the valve body 56C is opened by the suction force acting on the suction tool side inside the suction tool connecting portion 552. Thereby, air distribute | circulates from the connection tube 4C toward the exterior.
On the other hand, when the suction of air from the suction tool connection portion 552 side is stopped, the slit of the valve body 56C is closed, so that no air flows from the suction tool connection portion 552 side to the connection tube 4C side.

第４実施形態の生体試料移送装置１Ｃによれば、上述した（１）、（２）及び（５）の効果を奏する他、以下のような効果を奏する。   According to the biological sample transfer apparatus 1C of the fourth embodiment, in addition to the effects (1), (2), and (5) described above, the following effects can be obtained.

（６）ポート部を一方弁５Ｃにより構成した。これにより、吸引具により生体試料保存容器３の生体試料収容室３１１の内部に存在する空気を吸引する場合に、外部の空気が生体試料移送装置１Ｃの内部に侵入することを防げるので、生体試料移送装置１Ｃの内部の無菌状態を好適に保てる。   (6) The port portion is constituted by the one-way valve 5C. Accordingly, when the air present inside the biological sample storage chamber 311 of the biological sample storage container 3 is sucked by the suction tool, it is possible to prevent external air from entering the biological sample transfer apparatus 1C. The aseptic condition inside the transfer device 1C can be suitably maintained.

次に、本発明の第５実施形態に係る生体試料移送装置１Ｄについて、図１９及び図２０を参照しながら説明する。
第５実施形態の生体試料移送装置１Ｄは、主として、生体試料貯留容器を備えない点で、第１実施形態と異なる。第５実施形態では、生体試料移送装置１Ｄは、上流側チューブ４１Ｄの基端部に配置された連結部材９を備える。連結部材９は、筒状部９１と、この筒状部９１の外周面に配置された雄ネジ部９２と、を備える。
以上の連結部材９によれば、雄ネジ部９２に螺合可能な雌ネジ部を有するロック部を備えたロックシリンジ（図示せず）等を生体試料貯留容器２Ｄとして連結できる。 Next, a biological sample transfer apparatus 1D according to a fifth embodiment of the present invention will be described with reference to FIGS.
The biological sample transfer apparatus 1D of the fifth embodiment is different from the first embodiment mainly in that it does not include a biological sample storage container. In the fifth embodiment, the biological sample transfer device 1D includes the connecting member 9 disposed at the proximal end portion of the upstream tube 41D. The connecting member 9 includes a cylindrical portion 91 and a male screw portion 92 disposed on the outer peripheral surface of the cylindrical portion 91.
According to the connecting member 9 described above, a lock syringe (not shown) including a lock portion having a female screw portion that can be screwed into the male screw portion 92 can be connected as the biological sample storage container 2D.

第５実施形態の生体試料移送装置１Ｄによれば、上述した（１）から（３）の効果を奏する。   According to the biological sample transfer device 1D of the fifth embodiment, the effects (1) to (3) described above are achieved.

以上、本発明の好ましい各実施形態について説明したが、本発明は、上述した各実施形態に制限されるものではなく、適宜変更が可能である。
例えば、上述した各実施形態では、生体試料収容室３１１を真空成形により立体成形したが、これに限らない。即ち、生体試料収容室を、射出成形又は押し出し成形により立体成形してもよい。 As mentioned above, although each preferred embodiment of the present invention was described, the present invention is not limited to each embodiment mentioned above, and can be changed suitably.
For example, in each of the above-described embodiments, the biological sample storage chamber 311 is three-dimensionally formed by vacuum forming, but is not limited thereto. That is, the biological sample storage chamber may be three-dimensionally molded by injection molding or extrusion molding.

また、第５実施形態では、連結部材９に、生体試料貯留容器２Ｄとしてのロックシリンジを連結したが、これに限らない。即ち、連結部材に、生体試料貯留容器としての血液バッグを接続してもよい。また、連結部材として、びん針等を用いてもよい。   Moreover, in 5th Embodiment, although the lock syringe as biological sample storage container 2D was connected with the connection member 9, it is not restricted to this. That is, you may connect the blood bag as a biological sample storage container to a connection member. A bottle needle or the like may be used as the connecting member.

また、上述した実施態様では、吸引具取り外し工程Ｓ７の後に生体試料貯留工程Ｓ８を実施したが、これに限らない。即ち、例えば、生体試料貯留工程Ｓ８を第１閉止工程Ｓ１の前に実施してもよい。   In the above-described embodiment, the biological sample storing step S8 is performed after the suction tool removing step S7. However, the present invention is not limited to this. That is, for example, the biological sample storage step S8 may be performed before the first closing step S1.

また、第１実施形態では、上流側チューブ４１に取り付けたクランプにより上流側閉止部６を構成したが、これに限らない。即ち、生体試料導入チューブ２２２に取り付けられたクランプ２２４により上流側閉止部を構成してもよい。   Moreover, in 1st Embodiment, although the upstream closing part 6 was comprised with the clamp attached to the upstream tube 41, it is not restricted to this. That is, the upstream closing portion may be configured by the clamp 224 attached to the biological sample introduction tube 222.

１、１Ａ、１Ｂ、１Ｃ、１Ｄ 生体試料移送装置
２、２Ｄ 生体試料貯留容器
３ 生体試料保存容器
４，４Ｂ，４Ｃ，４Ｄ 接続チューブ
５，５Ａ，５Ｂ，５Ｃ ポート部
６ 上流側閉止部
７ 下流側閉止部
８ 三方活栓
９ 連結部材
４１，４１Ｂ，４１Ｃ，４１Ｄ 上流側チューブ
４２，４２Ｂ，４２Ｃ，４２Ｄ 下流側チューブ
４３Ｂ，４３Ｃ 分岐チューブ
５１ セプタム
１００ シリンジ（吸引具）
３１１ 生体試料収容室
５１２ 弾性部材
５１３ スリット部
Ｓ１ 第１閉止工程
Ｓ２ 吸引具接続工程
Ｓ３ 吸引工程
Ｓ４ 第２閉止工程
Ｓ５ 第１開放工程
Ｓ６ 気体供給工程
Ｓ７ 吸引具取り外し工程
Ｓ９ 生体試料移送工程 DESCRIPTION OF SYMBOLS 1, 1A, 1B, 1C, 1D Biological sample transfer device 2, 2D Biological sample storage container 3 Biological sample storage container 4, 4B, 4C, 4D Connection tube 5, 5A, 5B, 5C Port part 6 Upstream side closing part 7 Downstream Side closing part 8 Three-way stopcock 9 Connecting member 41, 41B, 41C, 41D Upstream tube 42, 42B, 42C, 42D Downstream tube 43B, 43C Branch tube 51 Septum 100 Syringe (suction tool)
311 Biological Sample Storage Chamber 512 Elastic Member 513 Slit Part S1 First Closing Step S2 Suction Device Connection Step S3 Suction Step S4 Second Closing Step S5 First Opening Step S6 Gas Supply Step S7 Suction Tool Removal Step S9 Biological Sample Transfer Step

Claims (9)

生体試料が貯留される生体試料貯留容器と、
可撓性を有する部材により構成されると共に立体成形により形成され、外力が加えられない状態では内部に空気が存在する立体形状を維持している生体試料収容室を有する生体試料保存容器と、
前記生体試料貯留容器と前記生体試料保存容器とを無菌的かつ気密に接続する接続チューブと、
前記接続チューブに設けられ、前記生体試料貯留容器、前記生体試料保存容器及び前記接続チューブの気密状態を維持しつつ、該接続チューブの内部の気体及び前記生体試料保存容器の内部の気体を外部に導出可能なポート部と、
前記ポート部よりも前記生体試料貯留容器側に設けられる上流側閉止部と、を備える生体試料移送装置。 A biological sample storage container in which the biological sample is stored;
A biological sample storage container having a biological sample storage chamber that is configured by a member having flexibility and is formed by three-dimensional molding, and maintains a three-dimensional shape in which air is present when no external force is applied;
A connection tube for connecting the biological sample storage container and the biological sample storage container aseptically and airtightly;
The gas inside the connection tube and the gas inside the biological sample storage container are provided to the outside while maintaining the airtight state of the biological sample storage container, the biological sample storage container, and the connection tube. Derivable port part,
A biological sample transfer device comprising: an upstream closing portion provided closer to the biological sample storage container than the port portion. 前記上流側閉止部は、前記接続チューブにおける前記ポート部よりも前記生体試料貯留容器側に設けられ該接続チューブにおける流体の流通を遮断する請求項１に記載の生体試料移送装置。   2. The biological sample transfer device according to claim 1, wherein the upstream-side closing portion is provided closer to the biological sample storage container than the port portion of the connection tube and blocks fluid flow in the connection tube. 前記ポート部よりも前記生体試料保存容器側に設けられ前記接続チューブにおける流体の流通を遮断する下流側閉止部を更に備える請求項１又は２に記載の生体試料移送装置。   The biological sample transfer apparatus according to claim 1, further comprising a downstream closing portion that is provided closer to the biological sample storage container than the port portion and blocks the flow of fluid in the connection tube. 前記ポート部は、膜状又は板状に形成された弾性部材と、該弾性部材に形成されたスリット部と、を有するセプタムを備える請求項１〜３のいずれかに記載の生体試料移送装置。   The biological sample transfer device according to claim 1, wherein the port portion includes a septum having an elastic member formed in a film shape or a plate shape and a slit portion formed in the elastic member. 前記ポート部は、前記接続チューブの内部から外部への気体の流通を許容し、外部から内部への気体の流通を遮断する一方弁を備える請求項１〜３のいずれかに記載の生体試料移送装置。   The biological sample transfer according to any one of claims 1 to 3, wherein the port portion includes a one-way valve that allows a gas flow from the inside to the outside of the connection tube and blocks a gas flow from the outside to the inside. apparatus. 前記接続チューブは、
前記生体試料貯留容器側に配置される上流側チューブと、
前記生体試料保存容器側に配置される下流側チューブと、
基端部が前記上流側チューブと前記下流側チューブとの接続部に接続される分岐チューブと、を備え、
前記ポート部は、前記分岐チューブの先端部に接続される請求項１〜５のいずれかに記載の生体試料移送装置。 The connecting tube is
An upstream tube disposed on the biological sample storage container side;
A downstream tube disposed on the biological sample storage container side;
A branch tube connected to a connection portion between the upstream tube and the downstream tube at a base end portion, and
The biological sample transfer device according to claim 1, wherein the port portion is connected to a distal end portion of the branch tube. 前記上流側閉止部及び前記下流側閉止部は、三方活栓により構成される請求項３に記載の生体試料移送装置。   The biological sample transfer device according to claim 3, wherein the upstream closing portion and the downstream closing portion are configured by three-way cocks. 請求項１〜７のいずれかに記載の生体試料移送装置を用いた生体試料移送方法であって、
前記上流側閉止部を閉止する第１閉止工程と、
前記ポート部に吸引具を接続する吸引具接続工程と、
前記吸引具により前記生体試料収容室が潰れるように変形するまで前記生体試料保存容器の内部の気体を吸引する吸引工程と、
前記吸引具を前記ポート部から取り外す吸引具取り外し工程と、
前記上流側閉止部を開放して前記生体試料貯留容器に収容された生体試料を前記生体試料保存容器に移送する生体試料移送工程と、を備える生体試料移送方法。 A biological sample transfer method using the biological sample transfer device according to any one of claims 1 to 7 ,
A first closing step for closing the upstream closing portion;
A suction tool connecting step of connecting a suction tool to the port portion;
A suction step of sucking the gas inside the biological sample storage container until the biological sample storage chamber is deformed so as to be crushed by the suction tool;
A suction tool removing step of removing the suction tool from the port portion;
A biological sample transfer method comprising: a biological sample transfer step of opening the upstream closing portion and transferring the biological sample stored in the biological sample storage container to the biological sample storage container. 請求項３〜７のいずれかに記載の生体試料移送装置を用いた生体試料移送方法であって、
前記上流側閉止部を閉止する第１閉止工程と、
前記ポート部に吸引具を接続する吸引具接続工程と、
前記吸引具により前記生体試料収容室が潰れるように変形するまで前記生体試料保存容器の内部の気体を吸引する吸引工程と、
前記下流側閉止部を閉止する第２閉止工程と、
前記上流側閉止部を開放する第１開放工程と、
前記吸引具に吸引された気体を前記生体試料貯留容器側に供給する気体供給工程と、
前記吸引具を前記ポート部から取り外す吸引具取り外し工程と、
前記下流側閉止部を開放して前記生体試料貯留容器に収容された生体試料を前記生体試料保存容器に移送する生体試料移送工程と、を備える生体試料移送方法。
A biological sample transfer method using the biological sample transfer device according to claim 3,
A first closing step for closing the upstream closing portion;
A suction tool connecting step of connecting a suction tool to the port portion;
A suction step of sucking the gas inside the biological sample storage container until the biological sample storage chamber is deformed so as to be crushed by the suction tool;
A second closing step of closing the downstream closing portion;
A first opening step of opening the upstream closing portion;
A gas supply step of supplying the gas sucked into the suction tool to the biological sample storage container side;
A suction tool removing step of removing the suction tool from the port portion;
A biological sample transfer method comprising: a biological sample transfer step of opening the downstream closing portion and transferring the biological sample stored in the biological sample storage container to the biological sample storage container.
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