JP6047105B2 - 除菌剤 - Google Patents
除菌剤 Download PDFInfo
- Publication number
- JP6047105B2 JP6047105B2 JP2013557541A JP2013557541A JP6047105B2 JP 6047105 B2 JP6047105 B2 JP 6047105B2 JP 2013557541 A JP2013557541 A JP 2013557541A JP 2013557541 A JP2013557541 A JP 2013557541A JP 6047105 B2 JP6047105 B2 JP 6047105B2
- Authority
- JP
- Japan
- Prior art keywords
- honey
- porphyromonas gingivalis
- royal jelly
- disinfectant
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000645 desinfectant Substances 0.000 title description 16
- 235000012907 honey Nutrition 0.000 claims description 29
- 241000605862 Porphyromonas gingivalis Species 0.000 claims description 24
- 229940109850 royal jelly Drugs 0.000 claims description 24
- 239000002324 mouth wash Substances 0.000 claims description 6
- 229940051866 mouthwash Drugs 0.000 claims description 4
- -1 oral ointment Substances 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 239000007937 lozenge Substances 0.000 claims 1
- 229940042125 oral ointment Drugs 0.000 claims 1
- 229940034610 toothpaste Drugs 0.000 claims 1
- 239000000606 toothpaste Substances 0.000 claims 1
- 208000028169 periodontal disease Diseases 0.000 description 11
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 210000000214 mouth Anatomy 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 230000003449 preventive effect Effects 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- DYKFCLLONBREIL-KVUCHLLUSA-N minocycline Chemical compound C([C@H]1C2)C3=C(N(C)C)C=CC(O)=C3C(=O)C1=C(O)[C@@]1(O)[C@@H]2[C@H](N(C)C)C(O)=C(C(N)=O)C1=O DYKFCLLONBREIL-KVUCHLLUSA-N 0.000 description 2
- 229960004023 minocycline Drugs 0.000 description 2
- 239000002674 ointment Substances 0.000 description 2
- 230000003239 periodontal effect Effects 0.000 description 2
- 201000001245 periodontitis Diseases 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000248349 Citrus limon Species 0.000 description 1
- 241000104430 Elsholtzia rugulosa Species 0.000 description 1
- 208000005888 Periodontal Pocket Diseases 0.000 description 1
- 241000605894 Porphyromonas Species 0.000 description 1
- 208000014151 Stomatognathic disease Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002079 cooperative effect Effects 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 239000000551 dentifrice Substances 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000008029 eradication Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000020185 raw untreated milk Nutrition 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
- A61K35/644—Beeswax; Propolis; Royal jelly; Honey
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
- A61K8/988—Honey; Royal jelly, Propolis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Insects & Arthropods (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Jellies, Jams, And Syrups (AREA)
- Cosmetics (AREA)
Description
1.ヤハシハチミツ及び/又はローヤルゼリーを含む、ポルフィロモナス・ジンジバリス除菌剤、
2.ヤハシハチミツ及び/又はローヤルゼリーを含む、歯周疾患の予防又は改善剤、
に関する。
1)ローヤルゼリー
RJ原乳20050407はジャパンローヤルゼリー株式会社(東京)から供与された。
2)皇蜜
ヤハシ純粋蜂蜜(4 582260 890998)はジャパンローヤルゼリー株式会社(東京)から供与された。
1) 皇蜜およびローヤルゼリー溶液の調整
皇蜜溶液は20%w/v(200mg/ml)となるようにリン酸緩衝生理食塩液(PBS)、またはポルフィロモナス・ジンジバリス用ブレインハートインフージョン(BHI)培地に溶解した後、0.22μmフィルターにて濾過滅菌し調製した。ローヤルゼリー溶液は50%w/vになるようにPBS中に懸濁し、4℃、12,000rpmで10分間遠心分離し、上清を0.22μmフィルターにて濾過滅菌し調製した。
2) ポルフィロモナス・ジンジバリスに対する皇蜜およびローヤルゼリーの最少発育阻止濃度(minimum inhibitory concentration;MIC)測定
ポルフィロモナス・ジンジバリスに対する皇蜜およびローヤルゼリーのMICを96ウェルマイクロプレートを用い、連続希釈法により測定した。
3)、皇蜜とローヤルゼリー(RJ)の協力作用
ローヤルゼリ(RJ)0、1、2、3、および5%と皇蜜0、2.5、5、10、20%を含有するポルフィロモナス・ジンジバリス用ブレインハートインフージョンを作成し、96ウェルマイクロプレートを用い測定した。
連続希釈法にてポルフィロモナス・ジンジバリスに対するMICを測定した。その結果陽性対照のABPCのMICは0.25μg/ml、皇蜜のMICは50mg/mlであった(図1)。 なお、この皇蜜溶液のpHは6.74であった。
次に、皇蜜とローヤルゼリー(RJ)の協力作用を検討した。その結果ローヤルゼリーが1%以上存在することにより皇蜜5%で完全にポルフィロモナス・ジンジバリスを死滅させることができた。ローヤルゼリーが5%存在する場合には、皇蜜2.5%で完全にポルフィロモナス・ジンジバリスを死滅させることができた。なお、ローヤルゼリーが存在しない場合には、皇蜜10%で完全にポルフィロモナス・ジンジバリスを死滅させることができた。
Claims (2)
- ヤハシハチミツ、又はヤハシハチミツ及びローヤルゼリーを含む、ポルフィロモナス・ジンジバリスを死滅させるための食品組成物。
- ヤハシハチミツ、又はヤハシハチミツ及びローヤルゼリーを含む、ポルフィロモナス・ジンジバリスを死滅させるための歯磨剤、口腔軟膏剤、洗口剤、含嗽剤、トローチ剤、又は口中錠。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012027328 | 2012-02-10 | ||
JP2012027328 | 2012-02-10 | ||
PCT/JP2013/052701 WO2013118759A1 (ja) | 2012-02-10 | 2013-02-06 | 除菌剤 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2013118759A1 JPWO2013118759A1 (ja) | 2015-05-11 |
JP6047105B2 true JP6047105B2 (ja) | 2016-12-21 |
Family
ID=48947520
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2013557541A Active JP6047105B2 (ja) | 2012-02-10 | 2013-02-06 | 除菌剤 |
Country Status (2)
Country | Link |
---|---|
JP (1) | JP6047105B2 (ja) |
WO (1) | WO2013118759A1 (ja) |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4546139B2 (ja) * | 2004-04-28 | 2010-09-15 | 株式会社サンギ | 口腔用組成物 |
JP2007277210A (ja) * | 2006-04-04 | 2007-10-25 | Masami Kanda | マヌカハニーを主成分とする歯周炎治療用剤およびサプリメント |
ES2618839T3 (es) * | 2007-04-16 | 2017-06-22 | Kikuji Yamaguchi | Agente descolonizante de Helicobacter pylori |
-
2013
- 2013-02-06 WO PCT/JP2013/052701 patent/WO2013118759A1/ja active Application Filing
- 2013-02-06 JP JP2013557541A patent/JP6047105B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
WO2013118759A1 (ja) | 2013-08-15 |
JPWO2013118759A1 (ja) | 2015-05-11 |
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