JP5958865B2 - 抗老化関連遺伝子発現促進剤 - Google Patents
抗老化関連遺伝子発現促進剤 Download PDFInfo
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- JP5958865B2 JP5958865B2 JP2014015776A JP2014015776A JP5958865B2 JP 5958865 B2 JP5958865 B2 JP 5958865B2 JP 2014015776 A JP2014015776 A JP 2014015776A JP 2014015776 A JP2014015776 A JP 2014015776A JP 5958865 B2 JP5958865 B2 JP 5958865B2
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Description
刈り取った大麦若葉10kgを圧搾し、得られた搾汁8kgをスプレードライにより乾燥、粉末化し、大麦若葉搾汁粉末0.5kgを得た。この粉末をM9緩衝液に懸濁し、0.45μmフィルターを通しろ過滅菌し、評価サンプルとした。なお、評価サンプル作製には、グリーンバイオアクティブ株式会社の製法(http://green-gbc.co.jp/technology/)及び特許第4600853号を参考にして行った。
1)M9緩衝液組成(1L当たり)
Na2HPO4 60g、KH2PO4 30g、NaCl 50g、1M MgSO4 1ml、2%ゼラチン 10mlをイオン交換水1Lに溶かし、121℃、20分間滅菌した。
2)S−Basal緩衝液組成(1L当たり)
NaCl 5.84g、KH2PO4 6.81gを、イオン交換水1Lに溶解し、121℃、20分間滅菌した。液温が60℃程度になるまで放冷し、コレステロール/エタノール(5mg/ml)を1ml加えた。
3)S−液体培地(1L当たり)
S−Basal緩衝液 1Lに、1M KH2PO4(pH6.0)10ml、1M CaCl2 3ml、1M MgSO4 3ml、Trace metal solution 10mlを加えた。(全て滅菌済みのものを使用)
4)Trace metal solution組成(1L当たり)
EDTA・2Na 1.86g、FeSO4・7H2O 0.69g、MnCl2・4H2O 0.20g、ZnSO4・7H2O 0.29g、CuSO4・5H2O 0.025gをイオン交換水1Lに溶かし、121℃、20分間滅菌した。
ヒト正常繊維芽細胞(Neonatal Normal Human Dermal Fibroblasts:NHDF)を用いて、SODの遺伝子発現解析を行った。ロンザジャパン株式会社より購入したNHDFを、牛胎児血清を10%含有するダルベッコ最小培地(DMEM)で2代継代し、ワーキングセルバンクを調製し、更に1代継代し、実験用として用いた。NHDFをセミコンフルエントまで培養し、1%の大麦若葉搾汁粉末を含む上記培地に交換した。実験コントロールには培地のみを用いた。その後37℃インキュベータ内で48時間培養を行った。
1%の大麦若葉搾汁粉末を懸濁したM9緩衝液を、0.45μmフィルターを用いてろ過滅菌し、評価サンプルとした。
Claims (1)
- 大麦若葉を有効成分として含有し、哺乳類体内の哺乳類SOD発現量を上昇させることを特徴とする哺乳類SOD(スーパ−オキサイドディスムターゼ)の遺伝子発現促進剤。
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JP2014015776A JP5958865B2 (ja) | 2014-01-30 | 2014-01-30 | 抗老化関連遺伝子発現促進剤 |
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JP2015140338A JP2015140338A (ja) | 2015-08-03 |
JP5958865B2 true JP5958865B2 (ja) | 2016-08-02 |
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Publication number | Priority date | Publication date | Assignee | Title |
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JP2004194616A (ja) * | 2002-12-20 | 2004-07-15 | Creatis Dam:Kk | 大麦若葉粉末エキスとしじみエキスを含有する健康食品。 |
JP3867680B2 (ja) * | 2003-04-01 | 2007-01-10 | ソニー株式会社 | 撮像装置および手振れ補正方法 |
JP2006321735A (ja) * | 2005-05-18 | 2006-11-30 | Creatis Dam:Kk | 大麦若葉エキス末に含まれるsodの活性酸素除外酵素による過酸化脂質の防止 |
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