JP5955499B2 - 皮膚コラーゲン産生促進剤 - Google Patents
皮膚コラーゲン産生促進剤 Download PDFInfo
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- JP5955499B2 JP5955499B2 JP2010222101A JP2010222101A JP5955499B2 JP 5955499 B2 JP5955499 B2 JP 5955499B2 JP 2010222101 A JP2010222101 A JP 2010222101A JP 2010222101 A JP2010222101 A JP 2010222101A JP 5955499 B2 JP5955499 B2 JP 5955499B2
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- Prior art keywords
- cystatin
- collagen production
- skin
- skin collagen
- degradation product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Description
(1)シスタチン及び/又はシスタチン分解物を有効成分とする皮膚コラーゲン産生促進剤。
(2)前記シスタチン分解物が、シスタチンをタンパク質分解酵素で分解して得られたものであることを特徴とする(1)記載の皮膚コラーゲン産生促進剤。
(3)前記タンパク質分解酵素が、トリプシン、パンクレアチン、キモトリプシン、ペプシン、パパイン、カリクレイン、カテプシン、サーモライシン、V8プロテアーゼから選択されるいずれか1種以上であることを特徴とする(2)記載の皮膚コラーゲン産生促進剤。
(4)前記シスタチン分解物が、分子量500以上、8000以下であることを特徴とする(1)〜(3)のいずれかに記載の皮膚コラーゲン産生促進剤。
(5)(1)〜(4)のいずれかに記載のシスタチン及び/またはシスタチン分解物を配合した皮膚コラーゲン産生促進用飲食品。
(6)(1)〜(4)のいずれかに記載のシスタチン及び/またはシスタチン分解物を配合した皮膚コラーゲン産生促進用化粧料。
(7)シスタチン及び/又はシスタチン分解物を経口摂取又は塗布することによる肌質の改善方法。
(8)シスタチン及び/又はシスタチン分解物を1日あたり10μg以上経口摂取するか、又は0.001〜2重量%になるよう塗布することによる肌質の改善方法。
実施例1で得られた画分A及び実施例3で得られた画分Cについて、ラットを用いた動物実験によりコラーゲン産生促進作用を調べた。7週齢のWistar系雄ラットを、生理食塩水投与群(A群)、実施例1で得られた画分Aをラット体重1kg当たり10μg投与する群(B群)、実施例1で得られた画分Aをラット体重1kg当たり100μg投与する群(C群)、実施例3で得られた画分Cをラット体重1kg当たり10μg投与する群(D群)、実施例3で得られた画分Cをラット体重1kg当たり100μg投与する群(E群)の5試験群(n=6)に分け、それぞれを毎日1回ゾンデで投与して10週間飼育した。皮膚のコラーゲン量については、ラットの真皮をNimniらの方法(Arch. Biochem. Biophys., 292頁, 1967年 参照)に準じて処理した後、可溶性画分に含まれるヒドロキシプロリン量を測定した。ヒドロキシプロリンはコラーゲンのみに含まれる特殊なアミノ酸で、コラーゲンを構成する全アミノ酸の約10%を占めることからコラーゲン量の推定ができる(浅野隆司ら,Bio Industory,12頁, 2001年 参照)。その結果を表1に示す。
実施例2で得られた画分B及び実施例3で得られた画分Dについて、正常ヒト線維芽細胞株〔白人女性の皮膚より採取されたCCD45SK(ATCCRL 1506)〕を用いた実験により皮膚コラーゲン産生促進作用を調べた。10容量%ウシ胎児血清(以下FBSと略記)含有変法イーグル培地(MEM、10‐101、大日本製薬社製)を用いて、正常ヒト線維芽細胞株を4×104個/ウエル/0.4mlとなるように24ウエルプレートに播種して、5%炭酸ガス、飽和水蒸気下、37℃で24時間培養した後、0.6容量%FBS含有MEM培地に置換した。そして、実施例2で得られた画分B及び実施例3で得られた画分Dを、各ウエルに0.1容量%となるように添加(n=6)して、24時間培養した後、β−アミノプロピオニトリルを50μg/ml、トリチウム−L−プロリンを1μCi/mlとなるように添加して、さらに24時間培養して培養液を得た。このようにして得られた培養液より、Websterらの方法(Analytical Biochemistry,220頁,1979年 参照)に従いコラーゲン画分を分画し、コラーゲン画分に取り込まれた放射能を測定した。なお、対照として、シスタチン及びシスタチン分解物を添加しないで同様の試験を行った。その結果を表2に示す。
実施例7で得られた化粧水及び実施例8で得られたクリームを用いて、実使用テストを行った。比較品としては、シスタチンを除いた以外は実施例7及び8と同じ配合のものを用いた。顔面のたるみや小ジワが認められる乾燥肌を有する成人女子20人を、それぞれ10人ずつ無作為に2群(A、B群)に、また、手に肌荒れが認められる女子20人を、それぞれ10人ずつ無作為に2群(C、D群)に分け、A群の顔面には本発明品の化粧水2gを、B群の顔面には比較品の化粧水2gを、C群の手指には本発明品のクリーム2gを、D群の手指には比較品のクリーム2gを、それぞれ1日2回通常の使用状態と同様に10日間塗布した。結果を表8に示す。
Claims (5)
- シスタチン及び/又はシスタチン分解物を有効成分とする真皮層の皮膚コラーゲン産生促進剤(飲食品の態様を除く)。
- 前記シスタチン分解物が、シスタチンをタンパク質分解酵素で分解して得られたものであることを特徴とする請求項1記載の皮膚コラーゲン産生促進剤。
- 前記タンパク質分解酵素が、トリプシン、パンクレアチン、キモトリプシン、ペプシン、パパイン、カリクレイン、カテプシン、サーモライシン、V8プロテアーゼから選択されるいずれか1種以上であることを特徴とする請求項2記載の皮膚コラーゲン産生促進剤。
- 前記シスタチン分解物が、分子量500以上、8000以下であることを特徴とする請求項1〜3のいずれかに記載の皮膚コラーゲン産生促進剤。
- 請求項1〜4のいずれかに記載のシスタチン及び/又はシスタチン分解物を配合した皮膚コラーゲン産生促進用化粧料。
Priority Applications (7)
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JP2010222101A JP5955499B2 (ja) | 2010-09-30 | 2010-09-30 | 皮膚コラーゲン産生促進剤 |
KR1020137009607A KR20130114141A (ko) | 2010-09-30 | 2011-09-29 | 피부 콜라겐 생성 촉진제 |
CA2810641A CA2810641A1 (en) | 2010-09-30 | 2011-09-29 | Skin collagen production-promoting agent |
US13/876,884 US20130225501A1 (en) | 2010-09-30 | 2011-09-29 | Skin collagen production-promoting agent |
PCT/JP2011/072363 WO2012043715A1 (ja) | 2010-09-30 | 2011-09-29 | 皮膚コラーゲン産生促進剤 |
CN2011800475151A CN103269707A (zh) | 2010-09-30 | 2011-09-29 | 皮肤胶原产生促进剂 |
MYPI2013000821A MY161768A (en) | 2010-09-30 | 2011-09-29 | Skin collagen production-promoting agent |
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JP (1) | JP5955499B2 (ja) |
KR (1) | KR20130114141A (ja) |
CN (1) | CN103269707A (ja) |
CA (1) | CA2810641A1 (ja) |
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CA2823505A1 (en) | 2011-01-26 | 2012-08-02 | Megmilk Snow Brand Co., Ltd. | Agent for improving peripheral sensitivity of skin |
JP5890100B2 (ja) | 2011-02-09 | 2016-03-22 | 雪印メグミルク株式会社 | 皮膚コラーゲン産生促進剤 |
JP2012188384A (ja) | 2011-03-10 | 2012-10-04 | Snow Brand Milk Products Co Ltd | 美肌剤 |
JP2013079216A (ja) | 2011-10-04 | 2013-05-02 | Snow Brand Milk Products Co Ltd | 感覚改善剤 |
CN114272361A (zh) * | 2021-12-17 | 2022-04-05 | 锐腾(苏州)生物科技有限公司 | 一种胶原蛋白分子单体皮肤涂敷液的组成和应用方法 |
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JP4607366B2 (ja) * | 2001-04-09 | 2011-01-05 | 花王株式会社 | 外用剤組成物 |
JP4291967B2 (ja) * | 2001-11-08 | 2009-07-08 | 雪印乳業株式会社 | 皮膚コラーゲン産生促進剤 |
DE60335665D1 (de) * | 2003-05-07 | 2011-02-17 | Snow Brand Milk Products Co Ltd | Mittel zur förderung der hautkollagenproduktion |
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WO2012043715A1 (ja) | 2012-04-05 |
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JP2012077019A (ja) | 2012-04-19 |
CA2810641A1 (en) | 2012-04-05 |
CN103269707A (zh) | 2013-08-28 |
KR20130114141A (ko) | 2013-10-16 |
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