JP5767716B2 - ルテイン含有シルクエキスを抽出する方法 - Google Patents
ルテイン含有シルクエキスを抽出する方法 Download PDFInfo
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- JP5767716B2 JP5767716B2 JP2013547406A JP2013547406A JP5767716B2 JP 5767716 B2 JP5767716 B2 JP 5767716B2 JP 2013547406 A JP2013547406 A JP 2013547406A JP 2013547406 A JP2013547406 A JP 2013547406A JP 5767716 B2 JP5767716 B2 JP 5767716B2
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- Prior art keywords
- lutein
- solution
- extraction solvent
- silk
- scouring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000004083 survival effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229910000406 trisodium phosphate Inorganic materials 0.000 description 1
- 235000019801 trisodium phosphate Nutrition 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
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- C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
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Description
ルテイン抽出方法100の第1の適用例では、シルクコクーンのサンプル(1±0.0002g)を、30mLの蒸留水によって、121℃で15分間精練した。その精練したコクーン(P1)を、0.1%のブチル化ヒドロキシトルエン(BHT)(w/v)を含む、30mLのヘキサン、エタノール、及び酢酸エチル(3:2:1、v/v/v)により、無色になるまで、4回抽出した。色素セリシン溶液(P2)のため、分割する前に、同じ抽出溶媒60mLを使用して、褐色ガラス分液漏斗で抽出を行ない、上部の相を得た。そして、上部の相を、真空下で乾燥するまで蒸発させ、乾固残渣が残った。乾固残渣は、HPLCグレードのn−ヘキサン及び酢酸エチル(3:1、v/v)に溶解し、0.45μmのフィルター膜でろ過される前に、5mLの最終量になるように調節した。そして、ろ過後の溶液は、後の分析のため、−20℃の窒素ガス内で保持された。精練したコクーンから抽出したカロテノイド、及び色素セリシン溶液は、HPLCにより同定された。色素エキス内のカロテノイド及びルテインの吸収スペクトラム及び濃度は、分光光度計を用い、エタノールにおいて測定された。
ルテイン抽出方法100の第2の最適例では、色素セリシン複合体は、5つの異なる処理により、黄色シルクコクーンから分離した。この5つの異なる処理とは、121℃下で、黄色シルクコクーンを15分間、30分間、60分間、90分間、120分間続いて精練することを含む。それぞれの処理において、黄色シルクコクーンは、暗所において、1:30の比例で、脱イオン水によって精練された。処理のためのそれぞれの持続時間の経過後、混合物を室温まで冷却し、精練溶液を分離した。脱イオン水を使用して、異なる処理から得た各々の色素セリシン溶液を、100mLの最終容積に調整した。すべての処理は3回行われた。色素セリシン溶液内の総タンパク質値は、ビシンコニン酸(BCA)タンパク質分析キットを用いて測定された。分析は、牛血清アルブミン(BSA)を標準品として、37℃で、30分間行われた。
ルテイン抽出方法100を用い、高温下、様々な時間分で、ルテインを抽出した。図9a〜9cは、(A)有機溶媒により抽出したルテインのクロマトグラム、(B)高速抽出器により100barの圧力下、100℃で、10分間抽出したルテインのクロマトグラム、(C)高速抽出器により100barの圧力下、100℃で、30分間抽出したルテインのクロマトグラム、(D)高速抽出器により100barの圧力下、150℃で、10分間抽出したルテインのクロマトグラムを示している。クロマトグラムの結果は、C18RP‐HPLCによって分析され、移動相としてアセトニトリル/メタノール(9:1,volume/volume)、及び酢酸エチルが用いられた。高温高圧におけるルテインの抽出(ルテイン抽出方法100の溶媒を用いた)は、高温高圧が、ルテインエキスを弱めない、または、損傷しないということが観察された。
ルテイン抽出方法から得られたルテインの効果は、ヒト網膜色素上皮(RPE)細胞培養で試験された。RPE細胞株、ARPE‐19(American Type Culture Collection、ATCC)は、DMEM/F‐12細胞培地にて、ウシ胎児血清(FBS)10%並びにペニシリン/ストレプトマイシンを用いて、二酸化炭素5%細胞培養器内で、37℃で培養された(トリプシン/エチレンジアミン四酢酸(EDTA)溶液による二次培養にて、3日〜4日毎に、細胞培地の変更あり)。結果は図10に示される。
マウスを使って、ルテイン抽出方法100を用いて黄色シルクコクーンから抽出したルテインと、他の原料からのルテインとの、比較効果を研究した。マウスは、一般的に、カロテノイド群の免疫調節効果の研究に用いられる実験動物である。この研究には、National Laboratory Animal Center of Mahidol University(タイ、バンコク)からの生後7週間のメスBALB/cマウスが使用され、Laboratory Animal Center of Faculty of Medical Science Naresuan University(タイ、ピトサヌロク(Phitsanulok))において、照明制御された部屋で、12時間:12時間の明暗サイクルで、25±1℃にて行われた。マウスは、この実験のため、滅菌蒸留水を与えた。
グループ1は、ルテインの溶媒のみをマウスに与え(PBS pH7.4の中に1% Tween80、溶剤対象群)、
グループ2は、黄色コクーンから抽出したルテインを、10mg/kg体重で、マウスに与え(sLT10)、
グループ3は、黄色コクーンから抽出したルテインを、20mg/kg体重で、マウスに与え(sLT20)、
グループ4は、マリーゴールドの花から抽出した市販のルテイン(キサントフィル≧95%、中国製)を、10mg/kg体重で、マウスに与え(cLT10)、
グループ5は、マリーゴールドの花から抽出した市販のルテイン(キサントフィル≧95%、中国製)を、20mg/kg体重で、マウスに与えた(cLT20)。
Claims (9)
- ルテインを抽出する方法であって、
精練溶液及び精練されたシルク材料を得るために、水相でシルク材料を精練するステップと、
前記精練溶液から精練された前記シルク材料を分離するステップと、
前記精練溶液及び精練されたシルク材料のそれぞれを抽出溶媒と接触させて前記精練溶液及び精練されたシルク材料からルテインを抽出するステップと、
接触された前記抽出溶媒を得るステップと、
得られた前記抽出溶媒を乾燥させてルテインを含む乾固残渣を得るステップと、を含み、
前記抽出溶媒は、ヘキサン、エチルアルコール及び酢酸エチルをそれぞれ3:2:1の重量比で含む混合液であり、
前記シルク材料は、黄色シルクコクーン、シルク糸及びシルク廃棄物のうちのいずれか1つ又はそれらの組み合わせである、方法。 - 請求項1に記載の方法において、
得られた前記乾固残渣を溶解して第2の溶液を得るステップと、
前記第2の溶液を濾過して該第2の溶液に溶解された純粋なルテインを得るステップと、
をさらに含む方法。 - 請求項1に記載の方法において、
前記抽出溶媒は、0.1%(w/v)のブチル化ヒドロキシトルエン及び/又はブチル化ヒドロキシアニソールをさらに含む方法。 - 請求項1に記載の方法において、
前記精練するステップは、前記シルク材料及び水相を加熱するステップ、
を含む方法。 - 請求項1に記載の方法において、
前記精練溶液から前記抽出溶媒を得るステップは、前記抽出溶媒と前記水相とを分割するステップ、
を含む方法。 - 請求項5に記載の方法において、
前記分割するステップは、
含水塩化ナトリウムを前記抽出溶媒に添加するステップ、
を含む方法。 - 請求項1に記載の方法において、
前記抽出溶媒を乾燥させるステップは、
無水硫酸ナトリウムを前記抽出溶媒に添加するステップ、
を含む方法。 - 請求項2に記載の方法において、
前記第2の溶液は、ヘキサン及び酢酸エチルを3:1の重量比で含む混合液である方法。 - 請求項1に記載の方法において、
シリンジフィルターによって第2の溶液をろ過する方法。
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US20090274660A1 (en) * | 1999-08-17 | 2009-11-05 | Immunopath Profile, Inc. | Pluripotent therapeutic compositions and uses thereof |
US6504067B1 (en) | 1999-11-24 | 2003-01-07 | Industrial Organica S.A. De C.V. | Process to obtain xanthophyll concentrates of high purity |
US20040267033A1 (en) | 2001-11-02 | 2004-12-30 | Rao Jonnalagadda Raghunath | Extraction of lutein from marigold meal |
US7173145B2 (en) | 2001-11-29 | 2007-02-06 | University Of Maryland, College Park | Process for extraction and purification of lutein, zeaxanthin and rare carotenoids from marigold flowers and plants |
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