JP5732078B2 - 音響エネルギーを使用して標本を処理するための処理システム - Google Patents
音響エネルギーを使用して標本を処理するための処理システム Download PDFInfo
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Description
本出願は、2010年3月4日に出願した米国仮特許出願第61/310,653号の利益を米国特許法第119条(e)項に従って主張するものである。この仮出願はすべて参照により本明細書に組み込まれる。
他の脱水プロトコルでは、組織を段階的に変わるアルコール濃度に曝し、最初に70%のエタノール/水混合物でリン酸緩衝液を取り除き、その後、95体積%および100体積%のエタノールの追加ステップで、固定された組織をさらに脱水する。組織は、実質的な収縮作用を受けうる(例えば、その元の体積の10%を超える)。収縮の量は、TOF測定結果を使用して決定することができる。組織の収縮は、結果の組織試料の変化(例えば、組織の硬化、組織の収縮など)によるTOF監視によって検出されうる。
Claims (14)
- 組織学的プロセスを生体試料に実行するステップであって、前記生体試料が、前記生体試料の状態を変えるために被検体から取り出されたものである、ステップと、
前記組織学的プロセスを前記生体試料に実行している間に音波の伝搬時間に基づき前記生体試料の状態を監視するステップと、を有し、
前記監視するステップは、
前記組織学的プロセスを前記生体試料に実行している間に前記音波を前記生体試料の少なくとも一部分の中に伝達するステップと、
前記プロセスの少なくとも一部を実行した後に前記生体試料の前記一部分の中を進行する前記音波の少なくとも一部の速度の変化を評価するステップと、
を含む方法。 - 前記生体試料の前記状態に基づき染色プロトコルを生成するステップをさらに含む請求項1に記載の方法。
- 前記生体試料の前記状態に基づき組織学的プロセスを前記生体試料に実行するステップであって、前記状態は、密集状態および固定状態のうちの少なくとも一方である、ステップをさらに含む請求項1に記載の方法。
- 前記音波を前記生体試料の厚さを横切って伝達するステップをさらに含む請求項1に記載の方法。
- 前記生体試料から前記音波の少なくとも一部を反射するステップと、
前記反射された音波を受け取るステップと、
前記生体試料の中に入った前記音波および前記反射された音波を評価して前記音波の速度の変化を評価するステップとをさらに含む請求項1に記載の方法。 - 前記状態を監視するステップは、
前記伝達される音波を生成するための出力信号と前記生体試料の中を進行した前記音波の受信信号との間の位相シフトを決定するステップを含む請求項1に記載の方法。 - 固定プロセスである前記プロセスによって引き起こされる前記音波の伝搬時間の変化を判定するステップをさらに含む請求項1に記載の方法。
- 前記音波の速度の変化を評価するステップと、
前記音波の速度の変化に関する情報を格納するステップと、
前記格納されている情報に基づきプロセスを別の生体試料に実行するステップとをさらに含む請求項1に記載の方法。 - 情報を格納するステップと、
前記音波の速度の変化を評価するステップと、
前記音波の速度の変化を前記格納されている情報と比較するステップと、
前記音波の速度の前記変化と前記格納されている情報との間の前記比較結果に基づき組織学的プロセスを制御するステップとをさらに含む請求項1に記載の方法。 - 補償アルゴリズムおよび平滑化器アルゴリズムのうちの少なくとも一方を使用してデータを解析するステップをさらに含む請求項1に記載の方法。
- プロセスを複数の生体試料に実行するステップと、
前記生体試料のそれぞれについて少なくとも1つの音速特性を得るステップと、
前記音速特性を前記各生体試料に相関させるステップと、
前記相関している音速特性を格納するステップと、
前記格納されている音速特性のうちの少なくとも1つに基づき組織学的プロセスを生体標本に実行するステップとを含む方法。 - 被検体から採取した生体試料を処理するための方法であって、
固定プロセスを前記生体試料に実行して生体試料の少なくとも一部を固定するステップと、
前記固定プロセスの少なくとも一部を実行した後に前記生体試料の中を進行する音波を使用して前記生体試料の中を進行する音の速度の変化を評価するステップと、
音の前記速度の前記変化の前記評価結果に基づき前記固定プロセスを調整するステップとを含む方法。 - 生体試料を評価するためのシステムであって、
音響エネルギーを被検体から採取された生体試料に通して出力するように構成された送信機と、
前記生体試料の少なくとも1つの音響特性が変えられた後に前記生体試料の中を進行した前記音響エネルギーを検出するように構成された受信機と、
前記送信機と前記受信機とに通信可能に結合され、前記音響エネルギーの伝搬時間に基づき前記生体試料の中を進行する前記音響エネルギーの速度の変化を評価するように構成されたコンピューティングデバイスとを備えるシステム。 - 前記コンピューティングデバイスは、前記音響エネルギーの前記速度の変化を評価して前記生体試料の固定状態を判定するように構成されている請求項13に記載のシステム。
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