JP5711879B2 - Fibroblast growth factor-7 production promoter, scalp / hair cosmetic containing the same, and food and drink - Google Patents
Fibroblast growth factor-7 production promoter, scalp / hair cosmetic containing the same, and food and drink Download PDFInfo
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- JP5711879B2 JP5711879B2 JP2009238855A JP2009238855A JP5711879B2 JP 5711879 B2 JP5711879 B2 JP 5711879B2 JP 2009238855 A JP2009238855 A JP 2009238855A JP 2009238855 A JP2009238855 A JP 2009238855A JP 5711879 B2 JP5711879 B2 JP 5711879B2
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Description
本発明は、毛乳頭細胞における線維芽細胞増殖因子−7(以下、FGF−7という。)発現を亢進させるFGF−7産生促進剤、及び、該FGF−7産生促進剤を含有する育毛・養毛に有効な頭皮・頭髪用化粧料、並びに、飲食物に関する。 The present invention relates to an FGF-7 production promoter that enhances the expression of fibroblast growth factor-7 (hereinafter referred to as FGF-7) in hair papilla cells, and hair growth / feeding containing the FGF-7 production promoter. The present invention relates to scalp and hair cosmetics effective for hair, and food and drink.
不規則な生活やストレス等の社会環境、食生活の変化により、抜け毛や脱毛、細毛、薄毛の悩みは、壮年男性だけでなく、若年層や女性にも広がっていることから、養育毛剤及び養育毛用化粧料に対する社会的期待は高まっている。これを受けて、育毛促進、保湿、フケやかゆみの予防、抗炎症、脱毛予防等を目的とした多くの養育毛剤が上市されている。 Due to irregular lifestyles, changes in social environment such as stress, and changes in dietary habits, hair loss, hair loss, fine hair, and thinning hair are spreading not only to older men but also to young people and women. Social expectations for cosmetics for nurturing hair are growing. In response to this, many hair-growing agents for the purpose of promoting hair growth, moisturizing, preventing dandruff and itching, anti-inflammatory, and preventing hair loss have been put on the market.
細胞成長因子の一種であるFGF−7(別名KGF)は、ケラチノサイト増殖因子、角質細胞増殖因子、線維芽細胞増殖因子などと呼ばれ、頭髪の薄毛部位では減少していることが知られている。 FGF-7 (also known as KGF), which is a kind of cell growth factor, is called keratinocyte growth factor, keratinocyte growth factor, fibroblast growth factor, etc., and is known to decrease in the thin hair region of the hair. .
これに関連して、毛包の細胞におけるケラチノサイト増殖因子(FGF−7)の発現を亢進させることにより毛髪の太毛化を維持・促進する方法、並びにアデノシンまたはその誘導体を含有するFGF−7の発現を亢進させる組成物を開示する文献(特許文献1)、コロソリン酸の配合により、線維芽細胞増殖因子−7(FGF−7)等の産生促進効果を有する頭髪化粧料を開示する文献(特許文献2)、KGFによる化学療法誘発生脱毛症の治療方法を開示する文献(特許文献3)が知られている。 In this connection, a method for maintaining and promoting hair thickening by enhancing the expression of keratinocyte growth factor (FGF-7) in hair follicle cells, and FGF-7 containing adenosine or a derivative thereof. A document disclosing a composition that enhances expression (Patent Document 1), a document disclosing hair cosmetics that have a production promoting effect such as fibroblast growth factor-7 (FGF-7) by the combination of corosolic acid (patent) Document 2), a document (Patent Document 3) that discloses a method for treating chemotherapy-induced alopecia with KGF is known.
そして、FGF−7は、頭髪の細毛、薄毛改善の極めて有効な因子であり、FGF−7産生促進効果を高める様々な方法が提案されているが、これらの方法をもってしても細毛、薄毛改善効果は十分なものではなかった。 FGF-7 is an extremely effective factor for improving fine hair and thinning hair, and various methods for enhancing the FGF-7 production promoting effect have been proposed. Even with these methods, fine hair and thinning hair can be improved. The effect was not sufficient.
本発明は、上記課題を解決するためになされたものであり、FGF−7産生に優れた効果を有するFGF−7産生促進剤の提供、及びそれを用いた頭皮・頭髪用化粧料、並びに飲用物の提供を目的とする。 The present invention has been made to solve the above problems, and provides an FGF-7 production promoter having an excellent effect on FGF-7 production, and a scalp / hair cosmetic using the same, and drinking The purpose is to provide goods.
本発明者らは、上記課題を解決するために鋭意研究を重ねた結果、植物由来成分のフォルスコリンは、FGF−7産生を促進する効果を有するが、これに特定の植物抽出エキスを併用することにより、フォルスコリンのFGF−7産生能力が飛躍的に増大することを見出し本発明を完成させた。 As a result of intensive studies to solve the above problems, the present inventors have found that forskolin, a plant-derived component, has an effect of promoting FGF-7 production, and a specific plant extract is used in combination therewith. Thus, the present inventors have found that the ability of forskolin to produce FGF-7 dramatically increases.
すなわち、本発明は、(A)フォルスコリンと(B)オウギエキス及び/又はチンピエキスからなるFGF−7産生促進剤を提供し、さらに、該FGF−7産生促進剤を含有するFGF−7産生促進効果の顕著な頭皮・頭髪用化粧料、及び飲食物を提供するものである。 That is, the present invention provides an FGF-7 production promoter comprising (A) forskolin and (B) an ogi extract and / or a chimpi extract, and further an FGF-7 production promoting effect comprising the FGF-7 production promoter. The present invention provides cosmetics for scalp and hair, and food and drink.
本発明の、フォルスコリンとオウギエキス及び/又はチンピエキスとからなるFGF−7産生促進剤は、毛乳頭細胞のFGF−7産生能力を劇的に促進する効果を有するものであり、これを頭皮・頭髪用化粧料あるいは飲食物に用いることで、細毛、薄毛の予防、更には毛髪の再生を図ることができる。 The FGF-7 production promoter comprising forskolin and an extract of an ogi and / or chimpi of the present invention has an effect of dramatically promoting the FGF-7 production ability of hair papilla cells. By using it in cosmetics for foods or foods and drinks, it is possible to prevent fine hair and thin hair, and to regenerate hair.
本発明のFGF−7産生促進剤は、フォルスコリンと、オウギエキス及びチンピエキスから選ばれる少なくとも1種の植物抽出エキスからなるものである。これらはいずれも、医薬又は植物エキスあるいはそれらを精製した成分であり、医薬又は民間薬、食品、化粧品の成分として一般的に用いられているものである。 The FGF-7 production promoter of the present invention comprises forskolin and at least one plant extract selected from an ogi extract and a chimpan extract. All of these are pharmaceuticals or plant extracts or components obtained by purifying them, and are generally used as components of pharmaceuticals, folk medicines, foods, and cosmetics.
本発明で使用するフォルスコリンとは、コレウスフォルスコリ(Coleus forskohlii)等のシソ科植物の塊根の抽出物から分離、精製され、構造決定された物質である。フォルスコリンは、上記植物の根からの抽出物を用いることができ、さらに、生化学試薬として市販されているものを用いることもできる。 Forskolin used in the present invention is a substance whose structure has been separated, purified and determined from the extract of tuberous roots of Lamiaceae plants such as Coleus forskohlii. As forskolin, an extract from the root of the plant can be used, and further, a commercially available biochemical reagent can be used.
本発明で使用するオウギエキスは、キバナオウギ、ナイモウオウギ、またはその他のマメ科植物の乾燥した根から得られる抽出物である。 The barley extract used in the present invention is an extract obtained from the dry roots of yellow-bellied, nayomous or other legumes.
本発明で使用するチンピエキスは、ウンシュウミカンの成熟した果皮から得られる抽出物である。 The chimney extract used in the present invention is an extract obtained from the mature peel of Citrus unshiu.
本発明で用いるオウギエキス、チンピエキス、コレウスフォルスコリエキス等の植物抽出エキスは、上記の各植物の各種部位、あるいは、その破砕物から溶媒抽出して得ることができる。 Plant extract extracts such as ogi extract, chimpi extract, and Coleus forskori extract used in the present invention can be obtained by solvent extraction from various parts of the above-mentioned plants or crushed materials thereof.
抽出溶媒に特に制限はなく、水、メタノール、エタノール、プロパノール、ブタノール等の低級1価アルコール、プロピレングリコール、ジプロピレングリコール、ブチレングリコール、グリセリン、1,3−ブタンジオール等の多価アルコール類、酢酸メチル、酢酸エチル等のエステル類、アセトン、メチルエチルケトン等のケトン類、クロロホルム、四塩化炭素等のハロゲン化炭化水素類、ジエチルエーテル、テトラヒドロフラン等のエーテル類、ヘキサン、ヘプタン、シクロヘキサン等の炭化水素類等一般に用いられる有機溶媒を挙げることができ、これらの中から選ばれる1種、または2種以上を混合して用いることができ、水と有機溶媒の混合溶媒を用いることができる。 There are no particular limitations on the extraction solvent, water, lower monohydric alcohols such as methanol, ethanol, propanol, and butanol, polyhydric alcohols such as propylene glycol, dipropylene glycol, butylene glycol, glycerin, and 1,3-butanediol, acetic acid Esters such as methyl and ethyl acetate, ketones such as acetone and methyl ethyl ketone, halogenated hydrocarbons such as chloroform and carbon tetrachloride, ethers such as diethyl ether and tetrahydrofuran, hydrocarbons such as hexane, heptane and cyclohexane The organic solvent generally used can be mentioned, 1 type chosen from these, or 2 or more types can be mixed and used, and the mixed solvent of water and an organic solvent can be used.
水と併用する有機溶媒としては炭素数4以下の低級アルコールが好ましく、特に水とエタノールの混合溶媒が好ましい。水と有機溶媒の混合溶媒を用いる場合、その混合割合は質量基準で、水:有機溶媒=10:90〜80:20とすることが好ましい。抽出温度としては15〜100℃が好ましく、15〜50℃での抽出がより好ましい。抽出温度を50℃以下とすることにより、植物抽出エキスの分解や変質を抑えることができる。抽出時間に制限はないが、抽出温度が50℃を超える場合、30秒〜5時間が好ましく、50℃以下で抽出する場合には5分〜7日間が好ましい。 As the organic solvent used in combination with water, a lower alcohol having 4 or less carbon atoms is preferable, and a mixed solvent of water and ethanol is particularly preferable. When using the mixed solvent of water and an organic solvent, it is preferable that the mixing ratio shall be water: organic solvent = 10: 90-80: 20 on a mass basis. As extraction temperature, 15-100 degreeC is preferable and extraction at 15-50 degreeC is more preferable. By making extraction temperature 50 degrees C or less, decomposition | disassembly and alteration of a plant extract can be suppressed. Although there is no restriction | limiting in extraction time, when extraction temperature exceeds 50 degreeC, 30 second-5 hours are preferable, and when extracting at 50 degrees C or less, 5 minutes-7 days are preferable.
前記各植物抽出エキスは、溶剤を揮発させた植物抽出エキスの液状品、凍結乾燥した粉末品として用いることができ、さらに、溶剤に溶解した液状品として用いることもできる。溶剤に溶解した液状品として用いる場合、エタノール、1,3−ブチレングリコール等の皮膚刺激性の低い溶媒を抽出溶媒に用いることが好ましく、これらと水との混合溶媒を抽出溶媒として用いることもできる。 Each plant extract can be used as a liquid product of a plant extract in which a solvent has been volatilized, a lyophilized powder product, or a liquid product dissolved in a solvent. When used as a liquid product dissolved in a solvent, a solvent having low skin irritation such as ethanol or 1,3-butylene glycol is preferably used as the extraction solvent, and a mixed solvent of these and water can also be used as the extraction solvent. .
本発明のFGF−7産生促進剤は、前記フォルスコリンとオウギエキス及びチンピエキスから選ばれる少なくとも1種とからなる。フォルスコリンは単独でも毛乳頭細胞のFGF−7産生促進効果を有するが、水に難溶の物質であるため、頭皮・頭髪用化粧料に対する配分量が制限され、毛乳頭細胞のFGF−7産生促進効果をもそれにより制限される。しかし、ここにオウギエキス及び/又はチンピエキスを併用することにより毛乳頭細胞でのFGF−7産生を飛躍的に促進する効果を得ることができる。 The FGF-7 production promoter of the present invention comprises the forskolin and at least one selected from an ogi extract and a chimpi extract. Forskolin alone has the effect of promoting FGF-7 production of dermal papilla cells, but because it is a substance that is sparingly soluble in water, the amount allocated to scalp and hair cosmetics is limited, and FGF-7 production of dermal papilla cells is limited. The promotion effect is also limited thereby. However, the combined use of the ogi extract and / or the chimney extract can provide an effect of dramatically promoting FGF-7 production in hair papilla cells.
本発明のFGF−7産生促進剤は毛乳頭細胞を賦活し、毛髪の細毛、薄毛の予防、毛髪の再生に寄与するものであり、本発明のFGF−7産生促進剤をそのまま養育毛剤として、あるいは頭皮・頭髪用化粧料に配合して、直接頭皮の毛乳頭細胞を賦活することができる。本発明のFGF−7産生促進剤は、通常の頭皮・頭髪用外用剤で使用されている薬剤等と組み合わせて使用することもでき、併用薬剤により本発明の効果がより発現しやすくなる。 The FGF-7 production promoter of the present invention activates dermal papilla cells and contributes to hair thinning and thinning hair prevention and hair regeneration. The FGF-7 production promoter of the present invention is used as it is as a nourishing hair growth agent. Alternatively, it can be directly added to the scalp / hair cosmetic to directly activate the hair papilla cells of the scalp. The FGF-7 production promoter of the present invention can also be used in combination with a drug or the like used in a normal scalp / hair external preparation, and the effect of the present invention is more easily exhibited by the combined drug.
本発明のFGF−7産生促進剤を配合できる頭皮・頭髪用化粧料の剤型に制限はなく、例えば、シャンプー、コンディショナー、トニック、スタイリング剤、ペースト剤、クリーム、ジェル、軟膏、ローション、乳液、パック、パウダー、ハップ剤等として、頭皮・頭髪に用いることができる。 There is no limitation on the dosage form of the scalp / hair cosmetic that can contain the FGF-7 production promoter of the present invention. For example, shampoo, conditioner, tonic, styling agent, paste agent, cream, gel, ointment, lotion, emulsion, It can be used on the scalp and hair as packs, powders, and haptics.
本発明のFGF−7産生促進剤の頭皮・頭髪用化粧料への配合量に特に制限はなく、任意の濃度で用いることができるが、フォルスコリンの好ましい配合量は、剤型中に0.001〜2質量%であり、より好ましくは、0.1〜1質量%である。 There is no restriction | limiting in particular in the compounding quantity to the scalp and hair cosmetics of the FGF-7 production promoter of this invention, Although it can be used by arbitrary density | concentrations, the preferable compounding quantity of forskolin is 0. It is 001-2 mass%, More preferably, it is 0.1-1 mass%.
オウギエキス、チンピエキスの好ましい配合量は、共に0.001〜5質量%であり、より好ましくは、共に、0.5〜5質量%である。植物抽出エキスの配合量が前記下限未満では、FGF−7産生促進による育毛効果があまり期待できず、前記上限を超えて使用しても、配合量に見合う効果が得られないおそれがある。 The preferred compounding amounts of the ogi extract and the chimpi extract are both 0.001 to 5% by mass, and more preferably 0.5 to 5% by mass. If the blending amount of the plant extract is less than the lower limit, the hair-growth effect due to the promotion of FGF-7 production cannot be expected so much, and even if it is used in excess of the upper limit, the effect commensurate with the blending amount may not be obtained.
フォルスコリンに対して、オウギエキスとチンピエキスの両方を共に併用する場合の好ましいオウギエキスとチンピエキスの配合量は、剤型中に、それぞれ0.001〜5質量%であり、より好ましくは0.5〜5質量%である。 The preferred blending amount of the ogi extract and the chimney extract when both the ogi extract and the chimney extract are used in combination with forskolin is 0.001 to 5% by mass, more preferably 0.5 to 5%, respectively. % By mass.
オウギエキスとチンピエキスとを同時に用いる場合の、オウギエキスとチンピエキスの配合割合に特に制限はなく、任意の割合で配合することができる。 There is no restriction | limiting in particular in the mixing | blending ratio of an ogi extract and a chimney extract when using an ogi extract and a chimpi extract simultaneously, It can mix | blend in arbitrary ratios.
本発明のFGF−7産生促進剤を頭皮・頭髪用化粧料に配合する場合、必要に応じて有機溶剤に溶解して用いることができる。ここで用いる有機溶剤としては、皮膚刺激性の低い前記エタノール、1,3−ブチレングリコール等が好ましい。 When the FGF-7 production promoter of the present invention is blended in a scalp / hair cosmetic, it can be dissolved in an organic solvent as required. As the organic solvent used here, the ethanol, 1,3-butylene glycol and the like having low skin irritation are preferable.
本発明の頭皮・頭髪用化粧料には、本発明の効果を損なわない範囲で、FGF−7産生促進剤に加え、頭皮・頭髪用化粧料として従来から使用されている公知の機能成分、例えば、保湿剤、エモリエント剤、血行促進剤、細胞賦活化剤、抗酸化剤、抗炎症剤、抗菌剤、美白剤、過酸化物抑制剤等を配合することができる。 In the scalp / hair cosmetic of the present invention, in addition to the FGF-7 production promoter, known functional ingredients conventionally used as a scalp / hair cosmetic, for example, within a range not impairing the effects of the present invention, , Moisturizers, emollients, blood circulation promoters, cell activators, antioxidants, anti-inflammatory agents, antibacterial agents, whitening agents, peroxide inhibitors and the like.
公知の機能成分として、グリセリン、ブチレングリコール、尿素、アミノ酸類等の保湿剤;スクワラン、マカデミアナッツ油、オリーブ油、ホホバ油、シリコン油等のエモリエント剤;ビタミンE類、トウガラシチンキ等の血行促進剤;核酸等の細胞賦活化剤、ジブチルヒドロキシトルエン(BHT)、ジブチルヒドロキシアニソール(BHA)、酢酸トコフェロール等の抗酸化剤;グリチルリチン、アラントイン等の抗炎症剤;ヒノキチオール、塩化ベンザルコニウム、クロルヘキシジン塩、パラヒドロキシ安息香酸エステル等の抗菌剤;アスコルビン酸等の美白剤;スーパーオキシドジスムターゼ(SOD)等の過酸化物抑制剤等、種々の機能成分を配合することができ、オウゴンエキス、イチョウエキス、胎盤抽出物、乳酸菌培養抽出物等の植物・動物・微生物由来の各種抽出物等も制限なく添加して使用することができる。 Known functional ingredients include humectants such as glycerin, butylene glycol, urea and amino acids; emollients such as squalane, macadamia nut oil, olive oil, jojoba oil, and silicone oil; blood circulation promoters such as vitamin E and red pepper tincture; Cell activating agents such as dibutylhydroxytoluene (BHT), dibutylhydroxyanisole (BHA), tocopherol acetate, etc .; anti-inflammatory agents such as glycyrrhizin, allantoin; hinokitiol, benzalkonium chloride, chlorhexidine salt, parahydroxy Various functional components such as antibacterial agents such as benzoates, whitening agents such as ascorbic acid, peroxide inhibitors such as superoxide dismutase (SOD), etc. can be blended, ougon extract, ginkgo biloba extract, placenta extract , Lactic acid bacteria culture extraction Various extracts from plants, animals, and microorganisms like or the like can be used by adding without limitation.
本発明のFGF−7産生促進剤を含有する頭皮・頭髪用化粧料に、さらにミノキシジル等の既知の育毛成分を加えることもできる。これにより効果的な育毛効果が期待される。 A known hair-growth component such as minoxidil can also be added to the scalp / hair cosmetic containing the FGF-7 production promoter of the present invention. Thereby, an effective hair-growth effect is expected.
本発明のFGF−7産生促進剤は、飲食物に配合することもでき、前記FGF−7産生促進剤の他に、通常食品に使用されている様々な材料を併用することができる。頭皮・頭髪用化粧料として用いることに加え、飲食物として体内に取り込むことで、毛乳頭細胞をより活性化し、FGF−7の産生が促進される。飲食物への配合量に特に制限はなく、任意の濃度で用いることができるが、フォルスコリンの好ましい配合量は、剤型中に0.001〜2質量%であり、より好ましくは、0.1〜1質量%である。オウギエキス、チンピエキスの好ましい配合量は、共に0.001〜5質量%であり、より好ましくは、共に、0.5〜5質量%である。植物抽出エキスの配合量が前記下限未満では、FGF−7産生促進による養育毛効果があまり期待できず、前記上限値を超えて使用しても使用量に見合う効果が得られないおそれがある。 The FGF-7 production promoter of the present invention can also be blended in foods and drinks, and in addition to the FGF-7 production promoter, various materials usually used in foods can be used in combination. In addition to being used as a scalp / hair cosmetic, it is incorporated into the body as a food and drink to activate the hair papilla cells more and promote the production of FGF-7. There is no restriction | limiting in particular in the compounding quantity to food-drinks, Although it can use by arbitrary density | concentrations, the preferable compounding quantity of forskolin is 0.001-2 mass% in a dosage form, More preferably, it is 0.00. It is 1-1 mass%. The preferred compounding amounts of the ogi extract and the chimpi extract are both 0.001 to 5% by mass, and more preferably 0.5 to 5% by mass. If the blended amount of the plant extract is less than the lower limit, the hair-growing effect due to the promotion of FGF-7 production cannot be expected so much, and even if it is used in excess of the upper limit, an effect commensurate with the amount used may not be obtained.
フォルスコリンに対して、オウギエキスとチンピエキスの両方を併用する場合、好ましいオウギエキス及びチンピエキスの配合量は、剤型中にそれぞれ0.001〜5質量%であり、より好ましくはそれぞれ0.5〜5質量%である。オウギエキスとチンピエキスとを同時に用いる場合の、オウギエキスとチンピエキスの配合割合にも特に制限はなく、任意の割合で配合することができる。 When both an ogi extract and a chimpi extract are used in combination with forskolin, a preferable blending amount of the ogi extract and the chimpi extract is 0.001 to 5% by mass in the dosage form, and more preferably 0.5 to 5% by mass, respectively. %. There is no particular limitation on the mixing ratio of the ogi extract and the chimpi extract when the ogi extract and the chimpi extract are used at the same time, and they can be mixed at an arbitrary ratio.
本発明の飲食物の剤型に制限はなく、例えば、ビスケット、クッキー、キャンディー、ガム、粉末、錠剤、カプセル剤等の固形製剤、飲料等の液体製剤、ゼリー等の半固形製剤等を例示することができる。 There is no limitation on the dosage form of the food and drink of the present invention, and examples include solid preparations such as biscuits, cookies, candies, gums, powders, tablets, capsules, liquid preparations such as beverages, semi-solid preparations such as jelly, and the like. be able to.
また、本発明の頭皮・頭髪用化粧料及び飲食物には、その剤型化のために界面活性剤、油脂類等の基材成分や、必要に応じて増粘剤、防腐剤、等張化剤、酸化防止剤、紫外線吸収剤、キレート剤、香料、着色料等、頭皮・頭髪用化粧料または飲食物に従来用いられている種々の添加物を併用できる。 In addition, the scalp / hair cosmetics and foods and beverages of the present invention include base ingredients such as surfactants and fats and oils, and, if necessary, thickeners, preservatives, isotonic agents. Various additives conventionally used for scalp / hair cosmetics or foods and drinks, such as an agent, an antioxidant, an ultraviolet absorber, a chelating agent, a fragrance, and a coloring agent can be used in combination.
本発明で使用できる界面活性剤に特に制限はなく、一般的な非イオン界面活性剤、陰イオン界面活性剤、陽イオン界面活性剤、両性界面活性剤を用いることができる。 The surfactant that can be used in the present invention is not particularly limited, and general nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants can be used.
例えば、高級アルコールのアルキレンオキサイド付加物、高級アルキルアミンのアルキレンオキサイド付加物、高級脂肪酸のアルキレンオキサイド付加物、高級脂肪酸アミドのアルキレンオキサイド付加物、多価アルコールの脂肪酸エステル、硬化ひまし油のアルキレンオキサイド付加物、ポリエチレングリコールソルビタンアルキルエステル、ステロール等のアルキレンオキサイド付加物等の非イオン界面活性剤;アルキル硫酸ナトリウム、アルキロイルメチルタウリンナトリウム、α−オレフィンスルホン酸ナトリウム、ポリオキシアルキレンアルキルエーテル硫酸ナトリウム等の陰イオン界面活性剤;塩化アルキルピリジニウム、塩化ジステアリルジメリルアンモニウム等の陽イオン界面活性剤;アミノプロピオン酸ナトリウム、アルキルポリアミノエチルグリシン等の両性界面活性剤が挙げられる。そして、これらの界面活性剤は1種又は2種以上を選択して使用することができる。 For example, alkylene oxide adduct of higher alcohol, alkylene oxide adduct of higher alkylamine, alkylene oxide adduct of higher fatty acid, alkylene oxide adduct of higher fatty acid amide, fatty acid ester of polyhydric alcohol, alkylene oxide adduct of hardened castor oil , Nonionic surfactants such as polyethylene oxide sorbitan alkyl esters, alkylene oxide adducts such as sterols; anions such as sodium alkyl sulfate, sodium alkyloxymethyl taurate, sodium α-olefin sulfonate, sodium polyoxyalkylene alkyl ether sulfate Surfactants; Cationic surfactants such as alkylpyridinium chloride and distearyl dimerylammonium chloride; Sodium aminopropionate, Al Le poly aminoethyl amphoteric surface active agents such as glycine and the like. And these surfactant can select and use 1 type (s) or 2 or more types.
本発明において使用可能な基材成分に特に制限はなく、例えば、オリーブ油、ツバキ油、アボカド油、マカデミアナッツ油、杏仁油、ホホバ油、スクワラン、スクワレン、馬油等、一般的に知られている油脂類を用いることができる。 There are no particular restrictions on the base component that can be used in the present invention, and examples include generally known fats and oils such as olive oil, camellia oil, avocado oil, macadamia nut oil, apricot oil, jojoba oil, squalane, squalene, and horse oil. Can be used.
本発明において使用可能な増粘剤に特に制限はなく、例えば、ポリビニルアルコール、ポリビニルアクリルアミド、ポリエチレングリコール、及びこれらの各種誘導体;ヒドロキシアルキルセルロース等のセルロース類及びその誘導体;デキストラン、ゼラチン、アラビアガム、トラガントガム等のガム類;カルボキシビニルポリマー等の水溶性高分子を用いることができる。 The thickener that can be used in the present invention is not particularly limited, and examples thereof include polyvinyl alcohol, polyvinyl acrylamide, polyethylene glycol, and various derivatives thereof; celluloses such as hydroxyalkyl cellulose and derivatives thereof; dextran, gelatin, gum arabic, Gums such as tragacanth gum; water-soluble polymers such as carboxyvinyl polymer can be used.
本発明において使用可能な防腐剤に特に制限はなく、例えば、パラヒドロキシ安息香酸エステル、パラオキシ安息香酸塩とその誘導体、フェノキシエタノール、ヒノキチオール、塩化ベンザルコニウム、クロルヘキシジン塩等を用いることができる。 There is no restriction | limiting in particular in the preservative which can be used in this invention, For example, parahydroxybenzoic acid ester, paraoxybenzoic acid salt and its derivative (s), phenoxyethanol, hinokitiol, benzalkonium chloride, chlorhexidine salt etc. can be used.
本発明において使用可能な等張化剤として、特に制限はなく、例えば、塩化ナトリウム、塩化カリウム、塩化カルシウム等の無機塩類を用いることができる。 There is no restriction | limiting in particular as an isotonic agent which can be used in this invention, For example, inorganic salts, such as sodium chloride, potassium chloride, calcium chloride, can be used.
本発明において使用可能な紫外線吸収剤として、特に制限はなく、例えば、パラアミノ安息香酸、ベンゾフェノン系紫外線吸収剤、ベンゾトリアゾール系紫外線吸収剤等を用いることができる。 There is no restriction | limiting in particular as an ultraviolet absorber which can be used in this invention, For example, a paraamino benzoic acid, a benzophenone series ultraviolet absorber, a benzotriazole type ultraviolet absorber etc. can be used.
本発明において使用可能なキレート剤として、特に制限はなく、例えば、エチレンジアミン四酢酸、フィチン酸、クエン酸及びこれらの水溶性塩等を用いることができる。 There is no restriction | limiting in particular as a chelating agent which can be used in this invention, For example, ethylenediaminetetraacetic acid, phytic acid, a citric acid, these water-soluble salts, etc. can be used.
以下、本発明を更に詳しく説明するために実施例によって説明するが、本発明はこれらの実施例により何ら限定されるものではない。 Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples.
・オウギエキスの製造方法
キバナオウギの根を50g採取し、25℃の50質量%エタノール水溶液375mlで、24時間振とうした。これをろ過し、得られた抽出液から水、エタノールを減圧留去後、凍結乾燥してオウギエキスを得た。得られたオウギエキスを、以下に示す実施例に使用した。
・チンピエキスの製造方法
ウンシュウミカンの果皮を50g採取し、25℃の50質量%エタノール水溶液375mlで、24時間振とうした。これをろ過し、得られた抽出液から水、エタノールを減圧留去後、凍結乾燥してチンピエキスを得た。得られたチンピエキスを、以下に示す実施例に使用した。
・コレウスフォルスコリエキスの製造方法
コレウスフォルスコリの塊根を50g採取し、25℃の50質量%エタノール水溶液375mlで、24時間振とうした。これをろ過し、得られた抽出液から水、エタノールを減圧留去後、凍結乾燥してコレウスフォルスコリエキスを得た。得られたコレウスフォルスコリエキスを、以下に示す実施例に使用した。
・フォルスコリン(試薬)
シグマアルドリッチジャパン(株)製のフォルスコリンを用いた。
-Production method of ogi extract 50g of roots of Echinocera were collected and shaken with 375ml of 50 mass% ethanol aqueous solution at 25 ° C for 24 hours. This was filtered, and water and ethanol were distilled off from the obtained extract under reduced pressure, followed by freeze-drying to obtain an ogi extract. The obtained barley extract was used in the following examples.
-Method for producing chimpi extract 50 g of persimmon peel was collected and shaken with 375 ml of a 50 mass% ethanol aqueous solution at 25 ° C for 24 hours. This was filtered, water and ethanol were distilled off from the resulting extract under reduced pressure, and then lyophilized to obtain a chimney extract. The obtained chimp extract was used in the following examples.
-Method for Producing Coleus Forskori Extract 50 g of tuberous root of Coleus forskori was collected and shaken with 375 ml of a 50 mass% aqueous ethanol solution at 25 ° C for 24 hours. This was filtered, and water and ethanol were distilled off from the resulting extract under reduced pressure, followed by lyophilization to obtain a Coleus forskori extract. The obtained Coleus forskori extract was used in the following examples.
・ Forskolin (reagent)
Forskolin manufactured by Sigma Aldrich Japan Co., Ltd. was used.
(I)FGF−7産生促進剤
1.細胞の培養
ヒト頭髪毛乳頭細胞(HFDPC、500,000cells/ml/本、TOYOBO社製)250,000cellsを、10%牛胎児血清(FBS)を含むGIBCO社製ダルベッコ改変イーグル培地(D−MEM)でシャーレに播種し、37℃、5%CO2の条件下で6日間培養した(この間、1日おきに培地を交換した)。
(I) FGF-7 production promoter Cultivation of cells Human hair hair papilla cells (HFDPC, 500,000 cells / ml / product, manufactured by TOYOBO) 250,000 cells containing GIBCO Dulbecco's modified Eagle medium (D-MEM) containing 10% fetal bovine serum (FBS) And inoculated in a petri dish at 37 ° C. under 5% CO 2 for 6 days (medium was changed every other day).
サブコンフルエント状態の毛乳頭細胞をトリプシン処理で剥離し、ウシI型コラーゲン処理した24ウェルプレートの各ウェルに4,000cellsを播種して、6日間培養した(この間、1日おきに培地を交換した)。 Subconfluent dermal papilla cells were detached by trypsin treatment, seeded with 4,000 cells in each well of a 24-well plate treated with bovine type I collagen, and cultured for 6 days (during this period, the medium was changed every other day). ).
6日間培養した毛乳頭細胞に表1に記載のとおりに調整したフォルスコリン、コレウスフォルスコリエキス、オウギエキス、チンピエキスを適宜添加したD−MEM培地(FBS無添加)を加えて24時間培養した。 D-MEM medium (without FBS) appropriately added with forskolin, coleus forskori extract, ogi extract, and chimpi extract prepared as shown in Table 1 was added to the hair papilla cells cultured for 6 days and cultured for 24 hours.
なお、フォルスコリン(試薬)はジメチルスルホキシドに溶解し、ジメチルスルホキシドの最終濃度が0.1質量%となるように培地中に添加した。オウギエキス、チンピエキス、コレウスフォルスコリエキスは50質量%エタノール水溶液を用いて25質量%、12.5質量%、10質量%、2.5質量%の溶液を調製し、エタノールの最終濃度が0.2質量%となるように培地中に添加した。これらを単独あるいは複数添加した培地をクリーンベンチ内で0.22 μmメンブランフィルターを用いて滅菌濾過したものを使用した。 Forskolin (reagent) was dissolved in dimethyl sulfoxide and added to the medium so that the final concentration of dimethyl sulfoxide was 0.1% by mass. For the ogi extract, chimpi extract and coleus forskori extract, a solution of 25% by mass, 12.5% by mass, 10% by mass and 2.5% by mass was prepared using a 50% by mass ethanol aqueous solution, and the final concentration of ethanol was 0.2. It added in the culture medium so that it might become mass%. A medium supplemented with one or more of these was sterilized and filtered using a 0.22 μm membrane filter in a clean bench.
2.FGF−7量の測定
FGF−7の定量には「Quantikine(登録商標)Human KGF(FGF−7) IMMUNOASSAY KIT」(R&D System社製)を使用した。
2. Measurement of FGF-7 Amount “Quantikine (registered trademark) Human KGF (FGF-7) IMMUNOASSAY KIT” (manufactured by R & D System) was used for quantification of FGF-7.
FGF−7抗体をコーティングしたウェルに、前記24時間培養後の培地100μLを添加し、室温で静置した。抗原抗体結合反応が終了した3時間後、各ウェルをKIT付属の洗浄液で5回洗浄して、未反応物を除去後、KGF Conjugate反応液(KITに付属のFGF−7検出抗体)を200μL添加し、室温で105分間静置してウェルのFGF−7に検出抗体を結合させた。 To the well coated with the FGF-7 antibody, 100 μL of the medium after 24 hours of culture was added and allowed to stand at room temperature. Three hours after the completion of the antigen-antibody binding reaction, each well was washed 5 times with a washing solution attached to KIT to remove unreacted substances, and then 200 μL of KGF Conjugate reaction solution (FGF-7 detection antibody attached to KIT) was added. The detection antibody was allowed to bind to FGF-7 in the well by allowing to stand at room temperature for 105 minutes.
結合反応終了後、未結合のFGF−7検出抗体を洗浄除去の上、発光試薬(テトラメチルベンジジン)を200μL添加して発色させ、30分間室温で静置後、KIT付属の反応停止液を50μL添加して測定試料とした。マイクロプレートリーダー(Model 550,Bio−Rad Laboratories社製)を用いて、吸光波長450nm(対照波長540nm)における吸光度を測定し、検量線を基に測定試料中のFGF−7濃度を算出した。 After completion of the binding reaction, the unbound FGF-7 detection antibody is washed and removed, and 200 μL of a luminescent reagent (tetramethylbenzidine) is added to cause color development. After standing at room temperature for 30 minutes, 50 μL of the reaction stop solution supplied with KIT is added. It added and it was set as the measurement sample. Absorbance at an absorption wavelength of 450 nm (control wavelength: 540 nm) was measured using a microplate reader (Model 550, manufactured by Bio-Rad Laboratories), and the FGF-7 concentration in the measurement sample was calculated based on the calibration curve.
上記試験の結果を表1に示す。
表1に示すように、FGF−7産生量は、0.004質量%のフォルスコリンで1.5倍に、0.1質量%のフォルスコリンで3.2倍になった。 As shown in Table 1, the production amount of FGF-7 was 1.5 times with 0.004 mass% forskolin and 3.2 times with 0.1 mass% forskolin.
オウギエキスのFGF−7産生量は、0.1〜0.05質量%で1.1倍に、チンピエキスのFGF−7産生量は、0.1〜0.05質量%で1.2倍にしかならなかった。 The amount of FGF-7 produced by the ogi extract is 0.1 to 0.05% by mass, 1.1 times, and the amount of FGF-7 produced by the chimney extract is only 1.2 times by 0.1 to 0.05% by mass. did not become.
しかしながら、フォルスコリンにオウギエキス及び/又はチンピエキスを併用することにより、FGF−7産生量は増大し、それぞれを0.1質量%併用した実施例3、6、9、13でのFGF−7産生量は、6〜7倍にまで達した。 However, the amount of FGF-7 produced is increased by using an ogi extract and / or a chimpi extract in combination with forskolin, and the amounts of FGF-7 produced in Examples 3, 6, 9, and 13 in which 0.1% by mass of each is used in combination. Reached 6-7 times.
以上のとおり、フォルスコリンと、オウギエキス及び/またはチンピエキス併用により、ヒト頭髪毛乳頭細胞でのFGF−7産生促進の相乗効果が確認された。 As described above, a synergistic effect of promoting FGF-7 production in human hair dermal papilla cells was confirmed by using forskolin in combination with an ogi extract and / or a chimney extract.
(II)頭皮・頭髪用化粧料
本発明の植物抽出エキスからなるFGF−7産生促進剤を、頭皮・頭髪用化粧料に配合し、毛髪に対する効果を確認した。
FGF−7産生促進剤を配合した化粧料(シャンプー、コンディショナー、ヘアトニック)の組成を表2−1、表2−2、表2−3に示す。オウギ、チンピの各植物抽出エキスは凍結乾燥したものを用い、フォルスコリンは試薬を用いた。なお、配合量の単位はgで、表中の精製水の「残量」とは全量を100gとする量である。
(II) Scalp / Hair Cosmetic A FGF-7 production promoter comprising the plant extract of the present invention was blended in a scalp / hair cosmetic, and the effect on hair was confirmed.
The compositions of cosmetics (shampoos, conditioners, hair tonics) containing FGF-7 production promoters are shown in Table 2-1, Table 2-2, and Table 2-3. The plant extracts of ogi and chimpi were lyophilized, and reagents were used for forskolin. The unit of the blending amount is g, and the “remaining amount” of purified water in the table is an amount that makes the total amount 100 g.
1.シャンプー
表2−1のA成分を混合の上75℃に加温し、撹拌しながら35℃まで冷却した。その後、撹拌を続けながらB成分を加えて、pHを4.5〜6と、粘度を1,000〜2,000mPa・sに調整した。さらにC成分を加えて、調整を終了した。
1. Shampoo The component A in Table 2-1 was heated to 75 ° C. after mixing and cooled to 35 ° C. with stirring. Then, B component was added, continuing stirring, and pH was adjusted to 4.5-6, and the viscosity was adjusted to 1,000-2,000 mPa * s. Further, the C component was added to complete the adjustment.
2.コンディショナー
表2−2のD成分、E成分をそれぞれ75℃に加温し、D成分をパドルミキサーで撹拌しながら、E成分を少量ずつ加えた。その後、パドルミキサーで撹拌しながら冷却して、45℃以下でF成分を加えて調整を終了した。
2. Conditioner D component and E component of Table 2-2 were each heated to 75 degreeC, and E component was added little by little, stirring D component with a paddle mixer. Then, it cooled, stirring with a paddle mixer, and F component was added at 45 degrees C or less, and adjustment was complete | finished.
3.ヘアトニック
表2−3のG成分、H成分、I成分の全てを40℃に加温して混合した。
3. Hair tonic G component, H component, and I component of Table 2-3 were all heated to 40 ° C. and mixed.
評 価
前記実施例14〜43、比較例9〜11の頭皮・頭髪用化粧料について、それぞれ30〜50歳の細毛化を訴える男女5名ずつの(11群×3処方)計330名に対して、シャンプー及びコンディショナーについては毎日の入浴時に頭髪に適量使用し、ヘアトニックについては毎日の入浴後又は朝に頭皮又は頭髪に適量使用して、これを6ヶ月間続けるモニター試験を行い、下記の基準にて評価を行った。なお、この期間中、シャンプーのモニター試験対象者は、シャンプーのみ本発明のシャンプーを使し、これ以外の頭髪用化粧用については、通常使用しているものをそのまま使用した。
Evaluation Regarding the scalp and hair cosmetics of Examples 14 to 43 and Comparative Examples 9 to 11, 5 men and women (11 groups x 3 prescriptions) each complaining of 30-50 years of hair thinning, totaling 330 people For shampoos and conditioners, use the appropriate amount for the hair at the time of daily bathing, and for hair tonics, use the appropriate amount for the scalp or hair after daily bathing or in the morning. Evaluation was performed based on the criteria. During this period, the subject of the shampoo monitor test used the shampoo of the present invention only for the shampoo, and used the hair shampoo other than that used in the usual manner.
コンディショナー、トニックのモニター試験対象者も、それぞれ、コンディショナー、トニック以外は、通常使用している頭髪用化粧品を使用した。 The conditioner and tonic monitor test subjects also used the usual hair cosmetics except for the conditioner and tonic, respectively.
評価は、側頭部を被験部位とし、使用前後での50本の毛髪の直径をシックネスゲージ7301(ミツトヨ社製)にて測定し、その平均毛髪径を求めて、比較することで効果を検証した。 Evaluation is performed by measuring the diameter of 50 hairs before and after use with a thickness gauge 7301 (manufactured by Mitutoyo Co., Ltd.) and comparing the average hair diameter by using the temporal region as a test site. did.
なお、使用期間中に皮膚の異常を訴えたものはいなかった。 None of the patients complained of skin abnormalities during the period of use.
結果を表2−1、表2−2、表2−3に示す。 The results are shown in Table 2-1, Table 2-2, and Table 2-3.
有 効 :使用前に比べ、毛髪径の平均が10%以上の増加
やや有効:使用前に比べ、毛髪径の平均が5%以上から10%未満の増加
効果無し:使用前に比べ、毛髪径の平均の増加が5%未満
悪 化 :使用前に比べ、毛髪径の平均が5%以上減少
Effective: Increase in average hair diameter by 10% or more compared to before use Slightly effective: Increase in average hair diameter from 5% to less than 10% compared to before use No effect: Hair diameter compared to before use The average increase in hair is less than 5% Deterioration: The average hair diameter decreases by 5% or more compared to before use
本発明のFGF−7産生促進剤を配合した実施例14〜43の頭皮・頭髪用化粧料では、毛髪径が太くなるか、やや太くなり、毛髪の細毛化の改善が認められ、特に1質量%のフォルスコリン、5質量%のオウギエキスとチンピエキスとを配合した実施例23、33、43では、すべての被験者に効果が確認された。一方、比較例9〜11の頭皮・頭髪用化粧料では、ほとんど変化が見られなかった。 In the scalp / hair cosmetics of Examples 14 to 43 containing the FGF-7 production promoter of the present invention, the hair diameter becomes thicker or slightly thicker, and an improvement in hair thinning is recognized. In Examples 23, 33, and 43 in which 5 % forskolin and 5% by weight ogi extract and chimpi extract were blended, the effects were confirmed for all subjects. On the other hand, in the scalp / hair cosmetics of Comparative Examples 9 to 11, almost no change was observed.
(III)飲食物の処方例
表3−1及び表3−2に示す組成で、FGF−7産生促進剤を配合した、飲食物(ゼリー、サプリメント(錠剤))を調整した。調整法は下記のとおりである。
オウギ、チンピの各植物抽出エキスは凍結乾燥したものを用い、フォルスコリンは試薬を用いた。
なお、配合量の単位はgで、表中の精製水の「残量」とは全量を100gとする量である。
(III) Food / Beverage Formulation Examples Foods / drinks (jellys, supplements (tablets)) containing FGF-7 production promoters were prepared with the compositions shown in Tables 3-1 and 3-2. The adjustment method is as follows.
The plant extracts of ogi and chimpi were lyophilized, and reagents were used for forskolin.
The unit of the blending amount is g, and the “remaining amount” of purified water in the table is an amount that makes the total amount 100 g.
1.ゼリー
表3−1のJ成分を、L成分の80℃のお湯に溶かし、K成分を溶解した後、よく混ぜ合わせた。型に10gずつ流し入れた後、冷蔵庫で固めて調整を終了した。
1. Jelly The J component in Table 3-1 was dissolved in 80 ° C. hot water of the L component, the K component was dissolved, and then mixed well. After pouring 10 g into the mold, it was hardened in a refrigerator to complete the adjustment.
2.サプリメント(錠剤)
表3−2のM成分とN成分を良く混ぜ合わせたものを、打錠機を用いてタブレット状に押し固め、錠剤を形成した。
2. Supplements (tablets)
What mixed M component and N component of Table 3-2 well was pressed and hardened using the tableting machine, and the tablet was formed.
評 価
実施例44〜63、比較例12、13の飲食物について、毛髪の細毛化の悩みを有する30〜40代の女性を対象としたモニター試験を実施した。
For the foods and drinks of Evaluation Examples 44 to 63 and Comparative Examples 12 and 13, a monitor test was conducted on women in their 30s and 40s who have trouble with thinning hair.
飲食物のそれぞれについて、女性10名ずつの(11群×2処方)計220名に対して、ゼリーについては、毎日の夕食後に100g摂取すること、サプリメント(錠剤)については、毎日の夕食後に5錠(1.5g)摂取すること以外は、通常の生活をするモニター試験を6ヶ月間続けた。評価は、側頭部を被験部位とし、使用前後での50本の毛髪の直径を、シックネスゲージ7301(ミツトヨ社製)にて測定し、その平均毛髪径を求めて、比較することで効果を検証した。 For each food and drink, 10 women (11 groups x 2 prescriptions) for a total of 220 people, for jelly, take 100g after daily dinner, for supplements (tablets), after daily dinner, 5 Except for taking tablets (1.5 g), the monitor test for normal life was continued for 6 months. The evaluation is performed by measuring the diameter of 50 hairs before and after use with a thickness gauge 7301 (manufactured by Mitutoyo Corporation) using the temporal region as a test site, and obtaining and comparing the average hair diameter. Verified.
なお、使用期間中に体調不良、皮膚の異常を訴えたものはいなかった。 None of the patients complained of poor physical condition or abnormal skin during the period of use.
結果を表3−1及び表3−2に示す。 The results are shown in Table 3-1 and Table 3-2.
有 効 :使用前に比べ、毛髪径の平均が10%以上の増加
やや有効:使用前に比べ、毛髪径の平均が5%以上から10%未満の増加
効果無し:使用前に比べ、毛髪径の平均の増加が5%未満
悪 化 :使用前に比べ、毛髪径の平均が5%以上減少
Effective: Increase in average hair diameter by 10% or more compared to before use Slightly effective: Increase in average hair diameter from 5% to less than 10% compared to before use No effect: Hair diameter compared to before use The average increase in hair is less than 5% Deterioration: The average hair diameter decreases by 5% or more compared to before use
本発明のFGF−7産生促進剤を配合した実施例44〜63の飲食物では、毛髪径が太くなるかやや太くなり、毛髪の細毛化の改善が認められ、特に1質量%のフォルスコリン、5質量%のオウギエキスとチンピエキスとを配合した実施例53、63では、すべての被験者に効果が確認された。一方、比較例12、13の飲食物では、頭髪にほとんど変化が認められなかった。 In the foods and drinks of Examples 44 to 63 formulated with the FGF-7 production promoter of the present invention, the hair diameter becomes thicker or slightly thicker, and an improvement in hair thinning is observed. In Examples 53 and 63 in which 5% by mass of an ogi extract and a chimpi extract were blended, the effect was confirmed for all subjects. On the other hand, in the foods and drinks of Comparative Examples 12 and 13, almost no change was observed in the hair.
フォルスコリンとオウギエキス及び/又はチンピエキスとからなる植物抽出エキスは、FGF−7産生促進剤として有用である。本発明のフォルスコリンとオウギエキス及び/又はチンピエキスとからなるFGF−7産生促進剤は、毛乳頭細胞で産生されるFGF−7の量を増大させ、その結果、細毛、薄毛の予防、緩和、改善、及び毛髪の再生をなすものであり、各種頭皮・頭髪用化粧料及び飲食物への利用が可能となる。 A plant extract composed of forskolin and an extract of ogi and / or chimpi is useful as an FGF-7 production promoter. The FGF-7 production promoter comprising forskolin of the present invention and an extract of ogi and / or chimpi extract increases the amount of FGF-7 produced in hair papilla cells, and as a result, prevention, alleviation and improvement of fine hair and thin hair. And hair regeneration, and can be used for various scalp / hair cosmetics and foods.
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