JP5706880B2 - 気体を含まない流体チャンバ - Google Patents
気体を含まない流体チャンバ Download PDFInfo
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- JP5706880B2 JP5706880B2 JP2012505265A JP2012505265A JP5706880B2 JP 5706880 B2 JP5706880 B2 JP 5706880B2 JP 2012505265 A JP2012505265 A JP 2012505265A JP 2012505265 A JP2012505265 A JP 2012505265A JP 5706880 B2 JP5706880 B2 JP 5706880B2
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0642—Filling fluids into wells by specific techniques
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
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- Chemical Kinetics & Catalysis (AREA)
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- Analytical Chemistry (AREA)
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Description
a.上述した流体チャンバを準備するステップと、
b.上述した流体チャンバの第1のチャネル(2)に液体を導入するステップと、
c.液体が、上述した流体チャンバの第2のチャネル(2)を通ってその充填された流体チャンバを出ていくように、流体チャンバを満たすステップと、
を含む方法に関する。
Claims (15)
- 流体チャンバに入る流体の通路として機能するのに適した第1のチャネル、及び
前記流体チャンバから出る流体の通路として機能するのに適した第2のチャネル、
と連絡し、
前記第1のチャネルと前記第2のチャネルとが互いに隣り合って位置付けられ、
1つの突出部が、前記流体チャンバ内に突き出し、
前記突出部が、前記第1のチャネルと前記第2のチャネルとの間に位置付けられている、流体チャンバ。 - 前記流体チャンバ内部の前記突出部の表面が滑らかである、請求項1に記載の流体チャンバ。
- 前記突出部が、円形状又は楕円形状である、請求項2に記載の流体チャンバ。
- 前記流体チャンバが、上から見ると円形又は楕円形の断面形状を有する円筒形であり、
前記第1のチャネル及び前記第2のチャネルが、円筒形の前記流体チャンバの側壁に接続されている、請求項1乃至3のいずれか1項に記載の流体チャンバ。 - 前記流体チャンバの直径が、約100μm乃至約10cmのレンジにあり、前記流体チャンバの高さが、約100μm乃至約1cmのレンジにある、請求項1乃至4のいずれか1項に記載の流体チャンバ。
- 円形状又は楕円形状の前記突出部の直径が、前記流体チャンバの直径の10倍小さく又は少なくとも約10倍小さい、請求項1乃至5のいずれか1項に記載の流体チャンバ。
- 円形状又は楕円形状の前記突出部の直径が、約10μm乃至約1cmのレンジにある、請求項1乃至6のいずれか1項に記載の流体チャンバ。
- 前記流体チャンバが、前記流体チャンバ内でポリメラーゼ連鎖反応を実施するのに適しているように構成される、請求項1乃至7のいずれか1項に記載の流体チャンバ。
- 前記流体チャンバ内の温度を制御する手段が、前記流体チャンバと連絡する、請求項1乃至8のいずれか1項に記載の流体チャンバ。
- 前記流体チャンバが、少なくとも1つの透明なセクションを有する、請求項1乃至9のいずれか1項に記載の流体チャンバ。
- 前記流体チャンバがポリプロピレンから作られている、請求項1乃至10のいずれか1項に記載の流体チャンバ。
- 液体による、気体を含まない充填のための請求項1乃至11のいずれか1項に記載の流体チャンバの使用。
- 液体により流体チャンバを完全に充填する方法であって、
a.請求項1乃至11のいずれか1項に記載の流体チャンバを準備するステップと、
b.前記流体チャンバの前記第1のチャネルに液体を導入するステップと、
c.前記液体が、前記液体チャンバの前記第2のチャネルを通って前記充填された流体チャンバを出て行くように、前記流体チャンバを充填するステップと、
を含む方法。 - 請求項1乃至11のいずれか1項に記載の流体チャンバを有する装置。
- 前記装置がカートリッジである、請求項14に記載の装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09157958.1 | 2009-04-15 | ||
EP09157958 | 2009-04-15 | ||
PCT/IB2010/051524 WO2010119377A1 (en) | 2009-04-15 | 2010-04-08 | A gas-free fluid chamber |
Publications (2)
Publication Number | Publication Date |
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JP2012523829A JP2012523829A (ja) | 2012-10-11 |
JP5706880B2 true JP5706880B2 (ja) | 2015-04-22 |
Family
ID=42334982
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012505265A Active JP5706880B2 (ja) | 2009-04-15 | 2010-04-08 | 気体を含まない流体チャンバ |
Country Status (10)
Country | Link |
---|---|
US (1) | US20120040445A1 (ja) |
EP (1) | EP2419218B1 (ja) |
JP (1) | JP5706880B2 (ja) |
KR (1) | KR101701715B1 (ja) |
CN (1) | CN102395431A (ja) |
AU (1) | AU2010238201B2 (ja) |
BR (1) | BRPI1006683A2 (ja) |
CA (1) | CA2758739C (ja) |
RU (1) | RU2525425C2 (ja) |
WO (1) | WO2010119377A1 (ja) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6637962B2 (ja) | 2014-04-24 | 2020-01-29 | ルシラ ヘルス インコーポレイテッド | 核酸増幅の比色検出方法 |
WO2016143377A1 (ja) * | 2015-03-09 | 2016-09-15 | ソニー株式会社 | マイクロチップ、マイクロチップのウェル、マイクロチップを用いた分析装置及びマイクロチップを用いた分析方法 |
US20160310948A1 (en) | 2015-04-24 | 2016-10-27 | Mesa Biotech, Inc. | Fluidic Test Cassette |
DK3430378T3 (da) | 2016-03-14 | 2022-10-24 | Lucira Health Inc | Indretninger og fremgangsmåder til modifikation af optiske egenskaber |
AU2017232344B2 (en) | 2016-03-14 | 2022-08-04 | Pfizer Inc. | Selectively vented biological assay devices and associated methods |
US11080848B2 (en) | 2017-04-06 | 2021-08-03 | Lucira Health, Inc. | Image-based disease diagnostics using a mobile device |
EP3612306A4 (en) * | 2017-04-21 | 2021-01-13 | Mesa Biotech, Inc. | FLUID TEST CASSETTE |
US10549275B2 (en) | 2017-09-14 | 2020-02-04 | Lucira Health, Inc. | Multiplexed biological assay device with electronic readout |
USD907232S1 (en) | 2018-12-21 | 2021-01-05 | Lucira Health, Inc. | Medical testing device |
US20220134327A1 (en) * | 2019-03-05 | 2022-05-05 | Lucira Health, Inc. | Bubble-free liquid filling of fluidic chambers |
USD953561S1 (en) | 2020-05-05 | 2022-05-31 | Lucira Health, Inc. | Diagnostic device with LED display |
USD962470S1 (en) | 2020-06-03 | 2022-08-30 | Lucira Health, Inc. | Assay device with LCD display |
Family Cites Families (12)
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ZA948564B (en) * | 1993-11-19 | 1995-07-26 | Bristol Myers Squibb Co | Liquid separation apparatus and method |
US6637463B1 (en) | 1998-10-13 | 2003-10-28 | Biomicro Systems, Inc. | Multi-channel microfluidic system design with balanced fluid flow distribution |
EP1080785A1 (en) * | 1999-09-04 | 2001-03-07 | F. Hoffmann-La Roche Ag | System for thermocycling of fluids in cartridges |
SK286037B6 (sk) * | 2001-05-09 | 2008-01-07 | Axis-Shield Asa | Testovací prístroj, testovacia náplň, testovacie zariadenie, jeho použitie a spôsob testovania |
US6843281B1 (en) | 2003-07-30 | 2005-01-18 | Agilent Techinologies, Inc. | Methods and apparatus for introducing liquids into microfluidic chambers |
DE10360220A1 (de) * | 2003-12-20 | 2005-07-21 | Steag Microparts Gmbh | Mikrostrukturierte Anordnung zur blasenfreien Befüllung zumindest eines Systems zur Ableitung von Flüssigkeiten, Vorrichtung mit einer solchen Anordnung und Befüllungsverfahren |
CN101039751B (zh) * | 2004-10-15 | 2010-05-05 | 西门子公司 | 用于在一次性卡盒中进行集成式自动dna或蛋白质分析的布置、所述卡盒的制备方法和使用所述卡盒进行dna或蛋白质分析的操作方法 |
CN101184983A (zh) * | 2005-03-16 | 2008-05-21 | 雅拓晶科生物***(私人)有限公司 | 用于传输、封闭和分析流体样品的方法和装置 |
US20070280856A1 (en) | 2006-06-02 | 2007-12-06 | Applera Corporation | Devices and Methods for Controlling Bubble Formation in Microfluidic Devices |
EP2111551A1 (en) * | 2006-12-20 | 2009-10-28 | Applied Biosystems, LLC | Devices and methods for flow control in microfluidic structures |
WO2008083687A1 (en) * | 2007-01-10 | 2008-07-17 | Scandinavian Micro Biodevices Aps | A microfluidic device and a microfluidic system and a method of performing a test |
JP2009250684A (ja) * | 2008-04-02 | 2009-10-29 | Rohm Co Ltd | マイクロチップ |
-
2010
- 2010-04-08 BR BRPI1006683A patent/BRPI1006683A2/pt not_active Application Discontinuation
- 2010-04-08 CA CA2758739A patent/CA2758739C/en active Active
- 2010-04-08 KR KR1020117026868A patent/KR101701715B1/ko active IP Right Grant
- 2010-04-08 US US13/264,231 patent/US20120040445A1/en not_active Abandoned
- 2010-04-08 RU RU2011146136/05A patent/RU2525425C2/ru active
- 2010-04-08 WO PCT/IB2010/051524 patent/WO2010119377A1/en active Application Filing
- 2010-04-08 JP JP2012505265A patent/JP5706880B2/ja active Active
- 2010-04-08 CN CN2010800167457A patent/CN102395431A/zh active Pending
- 2010-04-08 EP EP10717249.6A patent/EP2419218B1/en active Active
- 2010-04-08 AU AU2010238201A patent/AU2010238201B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JP2012523829A (ja) | 2012-10-11 |
KR101701715B1 (ko) | 2017-02-03 |
EP2419218B1 (en) | 2017-08-23 |
WO2010119377A1 (en) | 2010-10-21 |
AU2010238201B2 (en) | 2014-11-06 |
CN102395431A (zh) | 2012-03-28 |
CA2758739C (en) | 2016-11-08 |
KR20120017037A (ko) | 2012-02-27 |
RU2525425C2 (ru) | 2014-08-10 |
CA2758739A1 (en) | 2010-10-21 |
US20120040445A1 (en) | 2012-02-16 |
RU2011146136A (ru) | 2013-05-20 |
BRPI1006683A2 (pt) | 2016-04-12 |
AU2010238201A1 (en) | 2011-12-08 |
EP2419218A1 (en) | 2012-02-22 |
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