JP5467570B2 - Skin discrimination method - Google Patents

Skin discrimination method Download PDF

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JP5467570B2
JP5467570B2 JP2009263961A JP2009263961A JP5467570B2 JP 5467570 B2 JP5467570 B2 JP 5467570B2 JP 2009263961 A JP2009263961 A JP 2009263961A JP 2009263961 A JP2009263961 A JP 2009263961A JP 5467570 B2 JP5467570 B2 JP 5467570B2
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skin
depth
feeling
regularity
stratum corneum
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JP2011107027A5 (en
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昌枝 飯田
栄治 高橋
千明 浅海
正一郎 西川
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Pola Chemical Industries Inc
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Description

本発明は、肌を鑑別する技術に関し、更に詳しくは、角層細胞の積層規則性等を指標に肌の奥行き感を鑑別する技術に関する。   The present invention relates to a technique for discriminating skin, and more particularly, to a technique for discriminating a feeling of skin depth using, for example, the regularity of layered cells of horny layer cells as an index.

第三者によって、美しい肌であると認識されることは、女性のみならず多くの人の大きな願いの一つである。このため、美しい肌に見せるための化粧料や美容法の研究開発が盛んに行われている。しかしながら、肌の状態や特性は個人によって大きく異なり、さらに加齢や生活環境によっても変化するものであるため、化粧料の種類や化粧の方法、肌の手入れ法などを適切に選択するためには、対象となる肌が第三者にどのような状態でそのように見えるのかを高精度且つ客観的に判断することが必要である。例えば、デパートの化粧品売り場や、薬局、化粧品店の店頭においては、被験者の肌の状態や美しさの程度を評価する簡便な方法が求められている。このような肌の美しさは、シミ、シワやニキビなどで表現される、ネガティブな要因である「肌トラブルがない」と、キメの細かさやキメの方向の均一性、なめらかさ、しっとり感等で表現される、ポジティブな要因である、所謂、総合的な「肌の美しさ」が重要な要素であることが各種の調査結果より明らかにされている。   Recognizing that the skin is beautiful by a third party is one of the great wishes of many people as well as women. For this reason, research and development of cosmetics and beauty methods for showing beautiful skin are being actively conducted. However, since the skin condition and characteristics vary greatly depending on the individual, and also change depending on the aging and living environment, in order to properly select the type of cosmetics, makeup method, skin care method, etc. It is necessary to accurately and objectively determine in what state the target skin looks like to a third party. For example, in a department store cosmetics store, a pharmacy, or a cosmetic store, a simple method for evaluating the skin condition and the degree of beauty of a subject is required. Such skin beauty is expressed by spots, wrinkles, acne, etc. The negative factor is “there is no skin trouble”, the fineness of the texture, the uniformity of the texture direction, smoothness, moist feeling, etc. From various survey results, it is clarified that the so-called “skin beauty”, which is a positive factor expressed in the above, is an important factor.

かような「肌の美しさ」というものは、数々の要素が複合的に関連し合って形成れるものであり、肌の評価の専門家が、専門的知識に基づいて測定結果を分析したり、視覚的官能評価を行うことが必要であった。その場合には、複数の肌評価の専門家、その育成・訓練やさらに収集したデータ分析も必要であり、視覚的な「肌の美しさ」を的確、且つ簡便に評価することは困難であった。このため、「肌の美しさ」の客観的評価技術として、皮膚表面性状に着目した測定・評価法や装置が存する。例えば、複数の光の反射率を解析して皮膚の質感・つやを評価する方法・装置(例えば、特許文献1参照)、自然光の特性であるS偏光とP偏光を利用した皮膚の質感の観察方法・装置(例えば、特許文献2参照)、皮膚のステレオ画像から算出された凹凸量を指標に皮膚の質感評価するシステム(例えば、特許文献3参照)、皮膚表面反射光の年齢による成分強度の差を指標とした老化度合いの質感の簡便な測定法(例えば、特許文献4参照)、皮膚の起伏値や表色系画像信号に内在するフラクタル特性を用いた肌の美しさの推定方法(例えば、特許文献5参照)等が開示されている。かような方法は科学性を有する反面、測定装置高価であるという難点が存した。 Such “beauty of skin” is formed by a number of elements that are related to each other in a complex manner. Skin evaluation specialists analyze measurement results based on their specialized knowledge. Or visual sensory evaluation was necessary. In that case, it is also necessary to have multiple skin assessment specialists, their training and training, and further analysis of the collected data, making it difficult to accurately and easily assess visual “skin beauty”. It was. For this reason, as an objective evaluation technique for “skin beauty”, there are measurement / evaluation methods and apparatuses that focus on the skin surface properties. For example, a method / apparatus for evaluating the texture / gloss of skin by analyzing the reflectance of a plurality of light (see, for example, Patent Document 1), and observing the texture of skin using S-polarized light and P-polarized light, which are natural light characteristics Method / apparatus (for example, see Patent Document 2), system for evaluating the texture of the skin using the unevenness amount calculated from the stereo image of the skin as an index (for example, see Patent Document 3), component intensity of skin surface reflected light according to age A simple method of measuring the texture of the degree of aging using the difference in the index as an index (see, for example, Patent Document 4), and a method for estimating the beauty of the skin using the undulation value of the skin and the fractal characteristics inherent in the color system image signal ( For example, see Patent Document 5). While such a method has scientific properties, there is a problem that the measuring device is expensive.

一方、皮膚の構造や角層細胞に着目したアプローチも存する。例えば、干渉光現象を利用した低干渉光計測法によって皮膚の層構造である皮膚厚を計測しようとする装置・方法(例えば、特許文献6、7参照)が開示されている。また、ミクロな点に着目し、皮膚表面を構成している角層や角層細胞に着目し、顔の頬等の部位より粘着テープ等を用いてストリッピングにより角層細胞を採取し、染色によってその形状を明確にした後、角層細胞の面積、体積、厚さ、配列規則性、或いは角層細胞の剥がれ具合等を指標値にして、バリアー機能、肌質、肌性等の肌状態を鑑別しようとする注目すべき技術も多数存している。更には、角層細胞の膜厚と干渉光の波長(色味の変化、即ち虹色の輝き)の関係に注目し、この薄膜干渉によるミクロな虹色の輝きとマクロな「肌の美しさ」自身との相関関係を利用した肌の美しさの鑑別技術(例えば、特許文献8参照)も開示されている。   On the other hand, there are approaches that focus on the skin structure and stratum corneum cells. For example, an apparatus and a method (for example, see Patent Documents 6 and 7) that attempt to measure a skin thickness that is a layer structure of skin by a low interference light measurement method using an interference light phenomenon are disclosed. Focusing on microscopic points, focusing on stratum corneum and stratum corneum constituting the skin surface, collecting stratum corneum cells by stripping using adhesive tape etc. from the cheeks of the face, and staining After clarifying the shape of the skin, the area, volume, thickness, arrangement regularity of the stratum corneum cells, or the degree of peeling of the stratum corneum cells are used as index values, and the skin condition such as the barrier function, skin quality, skin property, etc. There are many notable technologies that try to distinguish between these. Furthermore, paying attention to the relationship between the thickness of stratum corneum cells and the wavelength of interference light (change in color, i.e., iridescence), the micro iridescence and macro "beauty of skin" by this thin film interference. "Skin beauty discrimination technology using a correlation with itself" (for example, see Patent Document 8) is also disclosed.

かような状況下において、本願発明者らは自然界の多層膜構造を有する生体、例えば、ホタテ貝、真珠や玉虫に注目した(図1参照)。図1に示すように、その断面は規則正しく並ぶ構造である、「積層規則性」を有している。かような「積層規則性」は、屈折率が異なる2つの層が交互に積層することにより、複雑な多重屈折光による多層膜干渉を生じさせ、例えば、真珠であれば奥行きのある輝きを、玉虫であれば美しい構造色を呈している。即ち、皮膚の角層構造に存するかような「積層規則性」を指標にすることにより、「肌の美しさ」、より正確には「肌の奥行き感」をより簡便且つ高精度に評価できる可能性に気がついた。かような角層細胞の「積層規則性」と「肌の奥行き感」との間には極めて高い相関関係を有することは全く知られていなかった。   Under such circumstances, the present inventors paid attention to a living body having a multilayered structure in nature, such as scallops, pearls, and iridescents (see FIG. 1). As shown in FIG. 1, the cross section has “stacking regularity”, which is a regularly arranged structure. Such “lamination regularity” causes two-layers having different refractive indexes to be alternately laminated, thereby causing multi-layer interference due to complicated multi-refracted light. If it is a beetle, it has a beautiful structural color. In other words, by using “stacking regularity” as if it exists in the stratum corneum of the skin as an index, it is possible to evaluate “skin beauty”, more precisely “skin depth” more simply and with high accuracy. I realized the possibility. It has not been known at all that there is an extremely high correlation between the “stacking regularity” and “skin depth” of such horny layer cells.

特開平07−071945号公報Japanese Patent Application Laid-Open No. 07-071945 特開平07−075629号公報Japanese Patent Application Laid-Open No. 07-075629 特開2000−088558号公報JP 2000-088558 A 特開2002−017689号公報JP 2002-017689 A 特開2007−252891号公報JP 2007-252891 A 特開平10−153550号公報Japanese Patent Laid-Open No. 10-153550 特開2006−000385号公報JP 2006-000385 A 特開2008−046061号公報JP 2008-046061 A 特開2006−095223号公報、JP 2006-095223 A, 特開2007−309805号公報JP 2007-309805 A N.Kashibuchi,J.Soc.Cosmet.Chem.Japan.23(2),143-154(1989)N. Kashibuchi, J. Soc. Cosmet. Chem. Japan. 23 (2), 143-154 (1989)

本発明はかような状況下で為されたものであり、肌状態の鑑別、特にこれまで明らか
にされていなかった「肌の美しさ」の大きな要因である「肌の奥行き感」を明らかにし
この「肌の奥行き感」を客観的な数値に置換する、簡便且つ高精度に評価できる技術を
提供することを課題とする。
The present invention has been made under such circumstances, and clarifies the “skin depth feeling” which is a major factor in the differentiation of skin conditions, in particular, “skin beauty” that has not been clarified so far. It is an object of the present invention to provide a technique that can replace the “feeling of skin depth” with an objective numerical value and that can be evaluated easily and with high accuracy.

この様な状況を鑑みて、本発明者らは、「肌の奥行き感」を明らかにし、この「肌の奥行き感」を客観的な数値に置換する、簡便且つ高精度に評価する技術を求めて鋭意研究努力を重ねた結果、角層細胞の「積層規則性」或いは配列規則性が「肌の美しさ」、より正確には「肌の奥行き感」と密接に関係すること、即ち、角層細胞の配列規則性が良いほど、内部反射光量が大きく、「奥行き感」を持った「肌の美しさ」を有することを見い出し、発明を完成させるに至った。即ち、本発明は、以下に示す技術に関する。 In view of such a situation, the present inventors have sought a technique for clarifying the “feeling of the skin depth” and replacing the “feeling of the skin depth” with an objective numerical value for simple and highly accurate evaluation. As a result of intensive research efforts, the “layering regularity” or arrangement regularity of stratum corneum cells is closely related to “beauty of skin”, more precisely “feeling of skin depth”. It has been found that the better the regularity of the layer cell arrangement, the greater the amount of internally reflected light and the “beauty of the skin” with a “depth”, and the invention has been completed. That is, this invention relates to the technique shown below.

(1)膚より採取した角層細胞の性状値である配列規則性及び/又は剥がれ具合を指標として、肌の奥行き感を鑑別することを特徴とする、肌の奥行き感の鑑別法。
(2)さらに、内部反射光量を指標として用いることを特徴とする、(1)に記載の肌の奥行き感の鑑別法。
(3)肌の奥行き感と前記角層細胞の性状値、或いは、肌の奥行き感と前記角層細胞の性状値及び内部反射光量との多変量解析の結果を指標として用いることを特徴とする、(1)又は(2)に記載の肌の奥行き感の鑑別法。
(4)(1)〜(3)何れか1つに記載の肌の奥行き感の鑑別法を用いることを特徴とする、メークの仕上がり感の鑑別法。
(1) as an index sequence regularity and / or peeling degree is property values of the stratum corneum cells collected from skin, characterized in that to differentiate the depth feeling of the skin, differentiation method of the depth feeling of the skin.
(2) The method for distinguishing a sense of skin depth according to (1), further comprising using the amount of internal reflection as an index.
(3) It is characterized by using as an index the skin depth sensation and the property value of the stratum corneum cells, or the result of multivariate analysis of the skin depth sensation and the property value of the stratum corneum cell and the amount of internal reflection. (1) Or the discrimination method of the feeling of depth of the skin as described in (2).
(4) A method for distinguishing a finished feeling of makeup, wherein the method for distinguishing a feeling of skin depth according to any one of (1) to (3) is used.

本発明によれば、簡便且つ高精度に、肌の美しさの大きな要因である「肌の奥行き感」を客観的な数値として評価できる。また、メークの仕上がり感についても同様に評価でき、メーク素材の仕上がり効果も評価できる According to the present invention, “feeling of skin depth”, which is a major factor of skin beauty, can be evaluated as an objective numerical value easily and with high accuracy. Moreover, the finish feeling of the makeup can be similarly evaluated, and the finish effect of the makeup material can also be evaluated .

本発明は皮膚より採取した角層細胞の性状値、例えば、角層細胞の配列規則性及び/又は剥がれ具合、更には内部反射光量とを指標に、該指標と「肌の奥行き感」との多変量解析の結果に基づく相関関係を利用して、「肌の奥行き感」を簡便且つ高精度に数値として推定することを特徴とする。以下に更に詳細に説明を加える。   The present invention uses a property value of a stratum corneum cell collected from the skin, for example, the regularity of the stratum corneum cell and / or the degree of peeling, and further the amount of internal reflection as an index, Using the correlation based on the result of multivariate analysis, the “feeling of skin depth” is estimated as a numerical value simply and with high accuracy. A more detailed description will be given below.

本発明の「肌の奥行き感」とは、肌のトラブル要因である、シミ、シワ、ニキビや赤み等が同じようなレベルであっても、「肌の美しさ」は全く異なる事象に基づいて形成された評価概念である(図2参照)。即ち、図2に示すように「肌の美しさ」に大差を生じさせる大きな要因は、後述する「積層規則性」の良否によって「内部反射光量」が影響された結果、肌の内部より感じる奥行き(立体感)のある輝きの程度が増減することに起因している。従って、ここでいう「肌の奥行き感」とは、肌の透明感と肌の内からの輝き感の両要素を持つ状態の程度と定義でき、この程度を数値化することで評価しうる。具体的には、予め訓練された複数の専門評価者によって繰り返し評価を行って、3〜10ランクの「肌の奥行き感」の基準画像表(図3参照)を作製しておけば、該基準画像表を用いて容易且つ精度良く評価される“Skin depth” of the present invention is based on a completely different phenomenon, even if skin trouble factors such as spots, wrinkles, acne and redness are at the same level. This is a formed evaluation concept (see FIG. 2). That is, as shown in FIG. 2, the major factor causing a great difference in “beauty of skin” is the depth felt from the inside of the skin as a result of “internal reflection light quantity” being influenced by the quality of “lamination regularity” described later. This is due to the increase or decrease in the degree of brightness with (three-dimensional effect). Therefore, the “feeling of the depth of the skin” here can be defined as the degree of the state having both the transparency of the skin and the feeling of brightness from within the skin, and this degree can be evaluated by quantifying. Specifically, after repeated evaluated by pre-trained plurality of expert evaluators, if prepared reference image table "sense of depth skin" of 3 to 10 ranks (see Fig. 3), the reference It is easily and accurately assessed using an image table.

前記角層細胞の採取方法としては、定法により皮膚よりテープストリッピング法によって採取した後、ゲンチアナバイレットやブリリアントグリーン等によって染色したものを用いることもできるし、また、染色せずに非染色のまま用いることもできる。角層細胞の性状値としては、角層細胞の配列規則性、面積、体積、厚さ或いは剥がれ具合等が例示でき、これらの内で、配列規則性及び/又は剥がれ具合が指標として特に好ましい。 As how to collect the stratum corneum cells was collected by the tape stripping method from the skin by a conventional method, it is possible to use those stained by Genchianabai O cmdlet and brilliant green, etc., also, non-staining without staining It can also be used as it is. Examples of the property value of the stratum corneum include the regularity of the stratum corneum cells, the area, the volume, the thickness, the degree of peeling, and the like. Among these, the ordering regularity and / or the degree of peeling is particularly preferable as an index.

前記配列規則性とは、1)角層細胞の大きさが揃っておりそのバラツキが少ないこと、2)細胞個々の形状が形の崩れていない六角形構造をしっかり維持し、角層細胞同士の配列が規則的であること、の2点をどの程度満たしているかということである。このような評価は、配列規則性のレベルを設定し、各種レベルの標準画像を複数揃えた標準セットを作製し、これらとの比較によって、例えば、配列規則性が良い〜配列規則性が悪いのように4〜10にランクを付けることができる(図4参照)。該配列規則性は、前記「積層規則性」の代替指標として用いることができことが既に知られている(非特許文献1参照)。また、前記剥がれ具合とは、テープストリッピングの際に、一層で剥離してくるか、数層が重なったまま剥離してくるかという程度であり、配列規則性と同様に4〜10にランクを付けることができる。   The arrangement regularity is as follows: 1) the size of the stratum corneum cells is uniform and there is little variation, and 2) the hexagonal structure in which the shape of each cell is not collapsed is firmly maintained, The degree to which the two points of the arrangement is regular is satisfied. In such an evaluation, the level of arrangement regularity is set, and a standard set in which a plurality of standard images of various levels are arranged is prepared. By comparison with these, for example, the arrangement regularity is good to the arrangement regularity is bad. 4-10 can be ranked as shown (see FIG. 4). It is already known that the arrangement regularity can be used as an alternative index of the “lamination regularity” (see Non-Patent Document 1). In addition, the degree of peeling refers to whether it peels in one layer or strips with several layers overlapping when tape stripping, and ranks 4 to 10 like the arrangement regularity. Can be attached.

かように標準画像を用いて、訓練された専門の評価者によってランクを付けることも可能であるが、角層細胞の配列規則性や剥がれ具合を自動的に評価できる方法・装置(例えば、特許文献9、特許文献10参照)を用いる方が高速且つ高精度であることからより好ましい。 Such a using a standard image, but it is also possible to kick with the rank by professional assessors trained, automatically method and equipment capable of evaluating the sequence regularity and peeling degree of corneocytes (e.g., It is more preferable to use Patent Literature 9 and Patent Literature 10) because of high speed and high accuracy.

角層の「積層規則性」を示す断面(図5参照)では、入射光に対し表面では表面反射光を、二層や三層などでは内部反射光を存し、多重屈折現象が生じる。「積層規則性」が良好である角層細胞、言い換えれば、配列規則性が良好であるほど、内部反射光量が多く、そのとき、肌の内部より感じる奥行き感(立体感)のある輝きの程度がより大きいことを本願発明者らは見出した(図6参照)。即ち、「肌の奥行き感」と角層細胞の性状値である配列規則性、剥がれ具合及び内部反射光量等の指標とは、良好な相関関係を有することが明らかになった。さらに、「肌の奥行き感」と前述の肌の指標とを多変量解析、例えば、重回帰分析、判別分析或いは数量化理論一類等を用いて予測式を予め作製しておけば、後述する実施例に示すように、前述の肌の指標より、簡便且つ高精度に、「肌の奥行き感」を客観的な数値として推定できる。 In cross-section showing a "stacked regularity" of the stratum corneum (see FIG. 5), the table Menhan Shako the surface with respect to the incident light, in such two-layer or three-layer resides an internal reflection light, the multi-refraction phenomenon occurs. The stratum corneum cells with good “regularity of layering”, in other words, the better the arrangement regularity, the more the amount of internal reflection, and the degree of brightness with a sense of depth (three-dimensional effect) that can be felt from the inside of the skin. The inventors of the present application have found that is larger (see FIG. 6). That is, it has been clarified that there is a good correlation between the “feeling of skin depth” and indices such as the arrangement regularity that is the property value of the stratum corneum, the degree of peeling, and the amount of internal reflection. Furthermore, if a prediction formula is prepared in advance using multivariate analysis such as “multiple regression analysis, discriminant analysis, or quantification theory” with respect to the “feeling of skin depth” and the above-described skin index, implementation described later As shown in the example, the “skin depth feeling” can be estimated as an objective numerical value easily and with high accuracy from the aforementioned skin index.

前記内部反射光量は、フォトニックラティス社製の偏光イメージングカメラ PI-100やBossa Nova Tech 社製の偏光イメージングカメラ SALSA等を利用して計測することができる。前記計測において、厳密な意味では、内部反射光量ではなく「皮膚反射光中の拡散光成分の比率」であるが、内部反射光量の代用として用いることができる。また、前記多変量解析は、SPSS社やSAS社等の市販ソフトウェア、或いはフリーソフトを用いることができる。   The amount of internal reflection can be measured using a polarization imaging camera PI-100 manufactured by Photonic Lattice, a polarization imaging camera SALSA manufactured by Bossa Nova Tech, or the like. In the measurement, in a strict sense, it is not the amount of internally reflected light but the “ratio of diffuse light component in skin reflected light”, but can be used as a substitute for the internally reflected light amount. The multivariate analysis can use commercial software such as SPSS or SAS, or free software.

メークやメーク素材の仕上がり感についても同様に評価できる。即ち、「肌の奥行き感」を上昇させるようなメーク素材、具体的なイメージを示すならば、積層規則性を有する構造粉体を作製して頬部の片方に塗布した後、頬部一対比較して「肌の奥行き感」を評価すればよい。「肌の奥行き感」で評価値がアップすれば、角層細胞の性状値が同じであることから、評価値のアップはメーク素材の効果であることが明らかである。さらに、後述する実施例で示すように、「肌の奥行き感」の予測式に当てはめれば、評価値のアップ分は角層細胞の性状値の向上分に相当する。言い換えれば、メーク素材による角層細胞の配列規則性の改善と言うこともでき、このようにして、メーク素材の機能評価やスクリーニングにも利用できる。かような鑑別や評価も本願発明の範疇に属するものである。 The finish of the makeup and makeup material can be similarly evaluated. That is, a makeup material that increases the “feel of skin depth”, and a specific image, a structured powder having lamination regularity is prepared and applied to one side of the cheek, and then a pair of cheeks are paired. The “skin depth” may be evaluated by comparison. If the evaluation value increases with “feeling of skin depth”, the property value of the stratum corneum cells is the same, so it is clear that the increase in the evaluation value is an effect of the makeup material. Furthermore, as shown in the examples described later, if applied to the prediction formula of “skin depth feeling”, the increase in the evaluation value corresponds to the improvement in the property value of the stratum corneum cells. In other words, it can be said that the makeup regularity of the stratum corneum cells is improved by the make material, and thus can be used for the function evaluation and screening of the make material. Such discrimination and evaluation also belong to the category of the present invention.

以下に実施例を挙げて、本発明について更に詳細に説明を加えるが、本発明がこれら実施例にのみ限定を受けないことは言うまでもない。   Hereinafter, the present invention will be described in more detail with reference to examples, but it is needless to say that the present invention is not limited to these examples.

<「肌の奥行き感」、角層細胞の性状値及び内部反射光量との関係>
年齢22〜50歳の10名の女性の頬部を用い、「肌の奥行き感」、角層細胞の性状値及び内部反射光量との関係について検討した。即ち、前記10名の女性の頬部中央(目尻と小鼻との交差点)について、洗顔後30分置いて、下記の方法と条件にて頬部中央(目尻と小鼻との交差点)の「肌の奥行き感」を評価した。さらに、内部反射光量を測定し、定法により採取された角層細胞の配列規則性と剥がれ具合の評価値を得た。これらのデータについて、Spearman順位相関分析を行い相互相関関係を検討した(表1参照)。表1の相関係数行列より、配列規則性が良好であるほど、「肌の奥行き感」が良好であることが分かる。
<Relationship between “feeling of skin depth”, property value of stratum corneum cells and amount of internal reflection>
Using the cheeks of 10 women aged 22 to 50 years old, the relationship between “feeling of skin depth”, the property value of stratum corneum cells and the amount of internal reflection was examined. That is, about the center of the cheek of the 10 women (intersection of the corner of the eye and the nose), 30 minutes after washing the face, the center of the cheek (intersection of the corner of the eye and the nose) of “skin "Depth" was evaluated. Furthermore, the amount of internal reflection was measured, and evaluation values of the arrangement regularity and the degree of peeling of the horny layer cells collected by a conventional method were obtained. About these data, Spearman rank correlation analysis was performed and cross-correlation was examined (refer Table 1). From the correlation coefficient matrix of Table 1, it can be seen that the better the arrangement regularity, the better the “skin depth”.

<計測方法と条件>
・「肌の奥行き感」:図3を利用して、3名の専門評価者による5ランク評価(5:奥行き感が非常にある〜1:奥行き感が全くない)の平均値
・角層細胞の配列規則性=4ランク評価(4:良い〜1:悪い)
・角層細胞の剥がれ具合=5ランク評価(5:剥離量が少ない〜1:剥離量が多い)
・内部反射光量(%):フォトニックラティス社製の偏光イメージングカメラPI-100
・解析方法:SPSS社製のSPSSシリーズの多変量解析ソフトウェア
<Measurement method and conditions>
・ "Skin depth": Using FIG. 3, the average value of 5-rank evaluation (5: Very deep feeling to 1: No sense of depth) by three professional evaluators. Arrangement regularity = 4 rank evaluation (4: good to 1: bad)
-Degree of detachment of stratum corneum = 5 rank evaluation (5: Less peel amount-1: More peel amount)
-Internal reflection amount (%): Polarized imaging camera PI-100 made by Photonic Lattice
-Analysis method: SPSS series multivariate analysis software manufactured by SPSS

<結果>

Figure 0005467570
<Result>
Figure 0005467570

<「肌の奥行き感」の推定方法>
実施例1に於いて、「肌の奥行き感」を目的変数に、角層細胞の性状値及び/又は内部反射光量を説明変数として、さらに重回帰分析を行った結果、以下のように良好な重相関係数、重回帰式を得た(図7参照)。これより、これらの回帰式を利用して、「肌の奥行き感」を簡便且つ精度良く推定できることが分かる。
・「肌の奥行き感」=0.605*「配列規則性」+1.177・・・(1)
r=0.842(p<0.01)
・「肌の奥行き感」=0.538*「配列規則性」+0.207*「剥がれ具合」+0.627・・・(2) r=0.854(p<0.01)
・「肌の奥行き感」=0.350*「配列規則性」+0.325*「剥がれ具合」+0.155*「内部反射光量」−12.775・・・(3)
r=0.891(p<0.01)
<Method for estimating “feeling of skin depth”>
In Example 1, the result of multiple regression analysis using “feeling of skin depth” as an objective variable and the property value of the stratum corneum cell and / or the amount of internal reflection as an explanatory variable was as follows. Multiple correlation coefficients and multiple regression equations were obtained (see FIG. 7). Thus, it can be understood that the “skin depth feeling” can be easily and accurately estimated using these regression equations.
・ "Skin depth" = 0.605 * "Sequence regularity" +1.177 (1)
r = 0.842 (p <0.01)
"Skin depth" = 0.538 * "regularity of arrangement" + 0.207 * "exfoliation" + 0.627 (2) r = 0.854 (p <0.01)
"Skin depth" = 0.350 * "arrangement regularity" + 0.325 * "peeling degree" + 0.155 * "internal reflection light quantity" -12.775 (3)
r = 0.891 (p <0.01)

<試験例>
30代女性2名を対象に、実施例1と同様に角層細胞の性状値(配列規則性と剥がれ具合)と「肌の奥行き感」の評価値を得た後、実施例2で得られた重回帰式(2)に代入して「肌の奥行き感」の推定値を求めた。別途、一般女性3名を対象に、「肌の奥行き感」の5ランクの基準写真を見せて同様に評価させた結果を、表2に併せて示す。これより、本願発明の推定式が非常に精度が良いことが分かる。
<Test example>
For two females in their 30s, the horny layer cell property values (alignment regularity and degree of peeling) and the evaluation value of “skin depth” were obtained in the same manner as in Example 1, and then obtained in Example 2. Substituting into the multiple regression equation (2), an estimated value of “feeling of skin depth” was obtained. Separately, Table 2 shows the results of the same evaluation by showing five ranks of “skin depth feeling” for three general women. From this, it can be seen that the estimation formula of the present invention is very accurate.

Figure 0005467570
Figure 0005467570

顧客と直接接する場所、例えば、お店やデパートの店頭において、顧客の角層情報より、簡便且つ客観的に肌の奥行き感やメークの仕上がり感を鑑別でき、肌や美容のカウンセリングに有用な情報を提供できる。   Information that is useful for skin and beauty counseling at a place that is in direct contact with the customer, for example, at a store or a department store, can easily and objectively distinguish the skin depth and makeup finish from the customer's stratum corneum information. Can provide.

ホタテ貝における積層規則性を示す図である(図面代用写真)。It is a figure which shows the lamination regularity in a scallop (drawing substitute photograph). 角層配列規則性が異なる頬部を示す図である(図面代用写真)。It is a figure which shows the cheek part from which stratum corneum arrangement regularity differs (drawing substitute photograph). 「肌の奥行き感」の10ランクの基準写真を示す図である(図面代用写真)。It is a figure which shows the reference | standard photograph of 10 ranks of "feeling of the depth of skin" (drawing substitute photograph). 配列規則性の4ランクの基準写真を示す図である(図面代用写真)。It is a figure which shows the reference | standard photograph of 4 ranks of arrangement regularity (drawing substitute photograph). 積層規則性と内部反射光との関係を示す図である。It is a figure which shows the relationship between lamination regularity and internal reflected light. 配列規則性内部反射光量との関係を示す図である(図面代用写真)。It is a figure which shows the relationship between arrangement regularity and the amount of internal reflections (drawing substitute photograph). 実施例2の結果で、肌の奥行き感(実測値)と角層の配列規則性、剥がれ具合及び内部反射光量による予測値との相関関係(検量線)を示す図である。It is a result which shows the correlation (calibration curve) with the result of Example 2 and the estimated value by skin depth feeling (actually measured value), the arrangement regularity of the stratum corneum, the degree of peeling, and the amount of internal reflection.

Claims (4)

皮膚より採取した角層細胞の性状値である配列規則性及び/又は剥がれ具合、
並びに肌の内部反射光量又はその代用値である皮膚反射光中の拡散光成分の比率
を指標として、肌の奥行き感を鑑別することを特徴とする、肌の奥行き感の鑑別法。
The regularity of the stratum corneum cells collected from the skin and / or the degree of peeling,
A skin depth sensation distinguishing method characterized by distinguishing the skin depth sensation by using as an index the ratio of diffuse light components in the skin reflected light that is the amount of internal reflection of the skin or a substitute value thereof .
肌の奥行き感と前記角層細胞の性状値、並びに内部反射光量又はその代用値である皮膚反射光中の拡散光成分の比率との多変量解析の結果を指標として用いることを特徴とする、請求項1に記載の肌の奥行き感の鑑別法。 And depth feeling skin, characterized by the use attribute value of the angle layer cells, as well as the results of the multivariate analysis of the ratio of the internal reflection light amount or diffused light component of the skin in the reflected light which is a substitute value as an index The method for distinguishing a feeling of skin depth according to claim 1 . 前記多変量解析の結果が、肌の奥行き感を目的変数とし、前記角層細胞の性状値、並びに肌の内部反射光量又はその代用値である皮膚反射光中の拡散光成分の比率を説明変数として重回帰分析し、得られた重回帰式であることを特徴とする、請求項2に記載の肌の奥行き感の鑑別法。The result of the multivariate analysis is that the skin depth is an objective variable, and the property value of the stratum corneum cell and the ratio of the diffuse light component in the skin reflected light that is the amount of internal reflection of the skin or a substitute value thereof are explanatory variables 3. The method of distinguishing a feeling of depth of skin according to claim 2, wherein the multiple regression equation is obtained by performing multiple regression analysis as follows. 請求項1〜3の何れか1項に記載の肌の奥行き感の鑑別法を用いることを特徴とする、メークの仕上がり感の鑑別法。   A method for distinguishing a finished feeling of a makeup, wherein the method for distinguishing a feeling of depth of skin according to any one of claims 1 to 3 is used.
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